Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis mediator mutants. Blomberg, J., Tasselius, V., Vergara, A., Karamat, F., Imran, Q. M., Strand, Å., Rosvall, M., & Björklund, S. Scientific Reports, 14(1):6771, March, 2024. Publisher: Nature Publishing Group![Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis mediator mutants [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediator mutants, med16, med18, med25 and cdk8, we studied how differences in regulation of their transcript and metabolite levels correlate to their responses to Pseudomonas syringae infection. We found that med16 and cdk8 were susceptible, while med25 showed increased resistance. Glucosinolate, phytoalexin and carbohydrate levels were reduced already before infection in med16 and cdk8, but increased in med25, which also displayed increased benzenoids levels. Early after infection, wild type plants showed reduced glucosinolate and nucleoside levels, but increases in amino acids, benzenoids, oxylipins and the phytoalexin camalexin. The Mediator mutants showed altered levels of these metabolites and in regulation of genes encoding key enzymes for their metabolism. At later stage, mutants displayed defective levels of specific amino acids, carbohydrates, lipids and jasmonates which correlated to their infection response phenotypes. Our results reveal that MED16, MED25 and CDK8 are required for a proper, coordinated transcriptional response of genes which encode enzymes involved in important metabolic pathways for Arabidopsis responses to Pseudomonas syringae infections.
@article{blomberg_pseudomonas_2024,
title = {Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of {Arabidopsis} mediator mutants},
volume = {14},
copyright = {2024 The Author(s)},
issn = {2045-2322},
url = {https://www.nature.com/articles/s41598-024-57192-x},
doi = {10.1038/s41598-024-57192-x},
abstract = {Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediator mutants, med16, med18, med25 and cdk8, we studied how differences in regulation of their transcript and metabolite levels correlate to their responses to Pseudomonas syringae infection. We found that med16 and cdk8 were susceptible, while med25 showed increased resistance. Glucosinolate, phytoalexin and carbohydrate levels were reduced already before infection in med16 and cdk8, but increased in med25, which also displayed increased benzenoids levels. Early after infection, wild type plants showed reduced glucosinolate and nucleoside levels, but increases in amino acids, benzenoids, oxylipins and the phytoalexin camalexin. The Mediator mutants showed altered levels of these metabolites and in regulation of genes encoding key enzymes for their metabolism. At later stage, mutants displayed defective levels of specific amino acids, carbohydrates, lipids and jasmonates which correlated to their infection response phenotypes. Our results reveal that MED16, MED25 and CDK8 are required for a proper, coordinated transcriptional response of genes which encode enzymes involved in important metabolic pathways for Arabidopsis responses to Pseudomonas syringae infections.},
language = {en},
number = {1},
urldate = {2024-04-02},
journal = {Scientific Reports},
author = {Blomberg, Jeanette and Tasselius, Viktor and Vergara, Alexander and Karamat, Fazeelat and Imran, Qari Muhammad and Strand, Åsa and Rosvall, Martin and Björklund, Stefan},
month = mar,
year = {2024},
note = {Publisher: Nature Publishing Group},
keywords = {Plant cell biology, Plant immunity, Plant molecular biology, Plant physiology, Plant sciences, Plant stress responses, Secondary metabolism, Transcriptional regulatory elements, Transcriptomics},
pages = {6771},
}
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Using four Arabidopsis Mediator mutants, med16, med18, med25 and cdk8, we studied how differences in regulation of their transcript and metabolite levels correlate to their responses to Pseudomonas syringae infection. We found that med16 and cdk8 were susceptible, while med25 showed increased resistance. Glucosinolate, phytoalexin and carbohydrate levels were reduced already before infection in med16 and cdk8, but increased in med25, which also displayed increased benzenoids levels. Early after infection, wild type plants showed reduced glucosinolate and nucleoside levels, but increases in amino acids, benzenoids, oxylipins and the phytoalexin camalexin. The Mediator mutants showed altered levels of these metabolites and in regulation of genes encoding key enzymes for their metabolism. At later stage, mutants displayed defective levels of specific amino acids, carbohydrates, lipids and jasmonates which correlated to their infection response phenotypes. 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