Transcriptome for Photobiological Hydrogen Production Induced by Sulfur Deprivation in the Green Alga <i>Chlamydomonas reinhardtii</i>. Nguyen, A. V., Thomas-Hall, S. R., Malnoë, A., Timmins, M., Mussgnug, J. H., Rupprecht, J., Kruse, O., Hankamer, B., & Schenk, P. M. Eukaryotic Cell, 7(11):1965–1979, November, 2008.
Transcriptome for Photobiological Hydrogen Production Induced by Sulfur Deprivation in the Green Alga <i>Chlamydomonas reinhardtii</i> [link]Paper  doi  abstract   bibtex   
ABSTRACT Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of sulfur-depleted hydrogen production. These studies were followed by real-time quantitative reverse transcription-PCR and protein analyses. The present work provides new insights into photosynthesis, sulfur acquisition strategies, and carbon metabolism-related gene expression during sulfur-induced hydrogen production. A general trend toward repression of transcripts encoding photosynthetic genes was observed. In contrast to all other LHCBM genes, the abundance of the LHCBM9 transcript (encoding a major light-harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodeling of the photosystem II light-harvesting complex as well as an important function of LHCBM9 under sulfur starvation and photobiological hydrogen production. This paper presents the first global transcriptional analysis of C. reinhardtii before, during, and after photobiological hydrogen production under sulfur deprivation.
@article{nguyen_transcriptome_2008,
	title = {Transcriptome for {Photobiological} {Hydrogen} {Production} {Induced} by {Sulfur} {Deprivation} in the {Green} {Alga} \textit{{Chlamydomonas} reinhardtii}},
	volume = {7},
	issn = {1535-9778, 1535-9786},
	url = {https://journals.asm.org/doi/10.1128/EC.00418-07},
	doi = {10/bv32ft},
	abstract = {ABSTRACT
            
              Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga
              Chlamydomonas reinhardtii
              at different time points before the onset and during the course of sulfur-depleted hydrogen production. These studies were followed by real-time quantitative reverse transcription-PCR and protein analyses. The present work provides new insights into photosynthesis, sulfur acquisition strategies, and carbon metabolism-related gene expression during sulfur-induced hydrogen production. A general trend toward repression of transcripts encoding photosynthetic genes was observed. In contrast to all other
              LHCBM
              genes, the abundance of the
              LHCBM9
              transcript (encoding a major light-harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodeling of the photosystem II light-harvesting complex as well as an important function of
              LHCBM9
              under sulfur starvation and photobiological hydrogen production. This paper presents the first global transcriptional analysis of
              C. reinhardtii
              before, during, and after photobiological hydrogen production under sulfur deprivation.},
	language = {en},
	number = {11},
	urldate = {2021-06-10},
	journal = {Eukaryotic Cell},
	author = {Nguyen, Anh Vu and Thomas-Hall, Skye R. and Malnoë, Alizée and Timmins, Matthew and Mussgnug, Jan H. and Rupprecht, Jens and Kruse, Olaf and Hankamer, Ben and Schenk, Peer M.},
	month = nov,
	year = {2008},
	pages = {1965--1979},
}

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