Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach. Sickel, W., Ankenbrand, M. J., Grimmer, G., Holzschuh, A., Härtel, S., Lanzen, J., Steffan-Dewenter, I., & Keller, A. BMC Ecology, 15:20, 2015.
Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach [link]Paper  doi  abstract   bibtex   
Meta-barcoding of mixed pollen samples constitutes a suitable alternative to conventional pollen identification via light microscopy. Current approaches however have limitations in practicability due to low sample throughput and/or inefficient processing methods, e.g. separate steps for amplification and sample indexing.
@article{sickel_increased_2015,
	title = {Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach},
	volume = {15},
	issn = {1472-6785},
	url = {http://dx.doi.org/10.1186/s12898-015-0051-y},
	doi = {10.1186/s12898-015-0051-y},
	abstract = {Meta-barcoding of mixed pollen samples constitutes a suitable alternative to conventional pollen identification via light microscopy. Current approaches however have limitations in practicability due to low sample throughput and/or inefficient processing methods, e.g. separate steps for amplification and sample indexing.},
	urldate = {2017-06-19},
	journal = {BMC Ecology},
	author = {Sickel, Wiebke and Ankenbrand, Markus J. and Grimmer, Gudrun and Holzschuh, Andrea and Härtel, Stephan and Lanzen, Jonathan and Steffan-Dewenter, Ingolf and Keller, Alexander},
	year = {2015},
	keywords = {DNA barcoding, High throughput sequencing, ITS2, Illumina MiSeq platform, NGS, Next generation sequencing, Osmia, Palynology, Pollination ecology},
	pages = {20},
}

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