Cytokinin response factors regulate PIN-FORMED auxin transporters. Simaskova, M., O'Brien, J. A., Khan, M., Van Noorden, G., Otvos, K., Vieten, A., De Clercq, I., Van Haperen, J. M. A., Cuesta, C., Hoyerova, K., Vanneste, S., Marhavý, P., Wabnik, K., Van Breusegem, F., Nowack, M., Murphy, A., Friml, J., Weijers, D., Beeckman, T., & Benkova, E. Nat Commun, 6(1):8717, November, 2015. Edition: 2015/11/07![Cytokinin response factors regulate PIN-FORMED auxin transporters [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.
@article{simaskova_cytokinin_2015,
title = {Cytokinin response factors regulate {PIN}-{FORMED} auxin transporters},
volume = {6},
issn = {2041-1723 (Electronic) 2041-1723 (Linking)},
url = {https://www.ncbi.nlm.nih.gov/pubmed/26541513},
doi = {10.1038/ncomms9717},
abstract = {Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.},
language = {en},
number = {1},
urldate = {2021-06-07},
journal = {Nat Commun},
author = {Simaskova, M. and O'Brien, J. A. and Khan, M. and Van Noorden, G. and Otvos, K. and Vieten, A. and De Clercq, I. and Van Haperen, J. M. A. and Cuesta, C. and Hoyerova, K. and Vanneste, S. and Marhavý, P. and Wabnik, K. and Van Breusegem, F. and Nowack, M. and Murphy, A. and Friml, J. and Weijers, D. and Beeckman, T. and Benkova, E.},
month = nov,
year = {2015},
note = {Edition: 2015/11/07},
keywords = {Arabidopsis, Arabidopsis Proteins/*genetics/metabolism, Chromatin Immunoprecipitation, Cytokinins/*metabolism, Gene Expression Regulation, Plant, Green Fluorescent Proteins, Indoleacetic Acids/*metabolism, Membrane Transport Proteins/*genetics/metabolism, Microscopy, Confocal, Plant Roots/metabolism, Plants, Genetically Modified, Real-Time Polymerase Chain Reaction, Response Elements, Signal Transduction, Transcription Factors/*genetics/metabolism},
pages = {8717},
}
Downloads: 0
{"_id":"9aSqSqKN3hFtLLbo5","bibbaseid":"simaskova-obrien-khan-vannoorden-otvos-vieten-declercq-vanhaperen-etal-cytokininresponsefactorsregulatepinformedauxintransporters-2015","author_short":["Simaskova, M.","O'Brien, J. A.","Khan, M.","Van Noorden, G.","Otvos, K.","Vieten, A.","De Clercq, I.","Van Haperen, J. M. A.","Cuesta, C.","Hoyerova, K.","Vanneste, S.","Marhavý, P.","Wabnik, K.","Van Breusegem, F.","Nowack, M.","Murphy, A.","Friml, J.","Weijers, D.","Beeckman, T.","Benkova, E."],"bibdata":{"bibtype":"article","type":"article","title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","volume":"6","issn":"2041-1723 (Electronic) 2041-1723 (Linking)","url":"https://www.ncbi.nlm.nih.gov/pubmed/26541513","doi":"10.1038/ncomms9717","abstract":"Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.","language":"en","number":"1","urldate":"2021-06-07","journal":"Nat Commun","author":[{"propositions":[],"lastnames":["Simaskova"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["O'Brien"],"firstnames":["J.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Khan"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Van","Noorden"],"firstnames":["G."],"suffixes":[]},{"propositions":[],"lastnames":["Otvos"],"firstnames":["K."],"suffixes":[]},{"propositions":[],"lastnames":["Vieten"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["De","Clercq"],"firstnames":["I."],"suffixes":[]},{"propositions":[],"lastnames":["Van","Haperen"],"firstnames":["J.","M.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Cuesta"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Hoyerova"],"firstnames":["K."],"suffixes":[]},{"propositions":[],"lastnames":["Vanneste"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Marhavý"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Wabnik"],"firstnames":["K."],"suffixes":[]},{"propositions":[],"lastnames":["Van","Breusegem"],"firstnames":["F."],"suffixes":[]},{"propositions":[],"lastnames":["Nowack"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Murphy"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Friml"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Weijers"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Beeckman"],"firstnames":["T."],"suffixes":[]},{"propositions":[],"lastnames":["Benkova"],"firstnames":["E."],"suffixes":[]}],"month":"November","year":"2015","note":"Edition: 2015/11/07","keywords":"Arabidopsis, Arabidopsis Proteins/*genetics/metabolism, Chromatin Immunoprecipitation, Cytokinins/*metabolism, Gene Expression Regulation, Plant, Green Fluorescent Proteins, Indoleacetic Acids/*metabolism, Membrane Transport Proteins/*genetics/metabolism, Microscopy, Confocal, Plant Roots/metabolism, Plants, Genetically Modified, Real-Time Polymerase Chain Reaction, Response Elements, Signal Transduction, Transcription Factors/*genetics/metabolism","pages":"8717","bibtex":"@article{simaskova_cytokinin_2015,\n\ttitle = {Cytokinin response factors regulate {PIN}-{FORMED} auxin transporters},\n\tvolume = {6},\n\tissn = {2041-1723 (Electronic) 2041-1723 (Linking)},\n\turl = {https://www.ncbi.nlm.nih.gov/pubmed/26541513},\n\tdoi = {10.1038/ncomms9717},\n\tabstract = {Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-06-07},\n\tjournal = {Nat Commun},\n\tauthor = {Simaskova, M. and O'Brien, J. A. and Khan, M. and Van Noorden, G. and Otvos, K. and Vieten, A. and De Clercq, I. and Van Haperen, J. M. A. and Cuesta, C. and Hoyerova, K. and Vanneste, S. and Marhavý, P. and Wabnik, K. and Van Breusegem, F. and Nowack, M. and Murphy, A. and Friml, J. and Weijers, D. and Beeckman, T. and Benkova, E.},\n\tmonth = nov,\n\tyear = {2015},\n\tnote = {Edition: 2015/11/07},\n\tkeywords = {Arabidopsis, Arabidopsis Proteins/*genetics/metabolism, Chromatin Immunoprecipitation, Cytokinins/*metabolism, Gene Expression Regulation, Plant, Green Fluorescent Proteins, Indoleacetic Acids/*metabolism, Membrane Transport Proteins/*genetics/metabolism, Microscopy, Confocal, Plant Roots/metabolism, Plants, Genetically Modified, Real-Time Polymerase Chain Reaction, Response Elements, Signal Transduction, Transcription Factors/*genetics/metabolism},\n\tpages = {8717},\n}\n\n","author_short":["Simaskova, M.","O'Brien, J. A.","Khan, M.","Van Noorden, G.","Otvos, K.","Vieten, A.","De Clercq, I.","Van Haperen, J. M. A.","Cuesta, C.","Hoyerova, K.","Vanneste, S.","Marhavý, P.","Wabnik, K.","Van Breusegem, F.","Nowack, M.","Murphy, A.","Friml, J.","Weijers, D.","Beeckman, T.","Benkova, E."],"key":"simaskova_cytokinin_2015","id":"simaskova_cytokinin_2015","bibbaseid":"simaskova-obrien-khan-vannoorden-otvos-vieten-declercq-vanhaperen-etal-cytokininresponsefactorsregulatepinformedauxintransporters-2015","role":"author","urls":{"Paper":"https://www.ncbi.nlm.nih.gov/pubmed/26541513"},"keyword":["Arabidopsis","Arabidopsis Proteins/*genetics/metabolism","Chromatin Immunoprecipitation","Cytokinins/*metabolism","Gene Expression Regulation","Plant","Green Fluorescent Proteins","Indoleacetic Acids/*metabolism","Membrane Transport Proteins/*genetics/metabolism","Microscopy","Confocal","Plant Roots/metabolism","Plants","Genetically Modified","Real-Time Polymerase Chain Reaction","Response Elements","Signal Transduction","Transcription Factors/*genetics/metabolism"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs","fvfkWcShg3Mybjoog","Tu3jPdZyJF3j547xT"],"keywords":["arabidopsis","arabidopsis proteins/*genetics/metabolism","chromatin immunoprecipitation","cytokinins/*metabolism","gene expression regulation","plant","green fluorescent proteins","indoleacetic acids/*metabolism","membrane transport proteins/*genetics/metabolism","microscopy","confocal","plant roots/metabolism","plants","genetically modified","real-time polymerase chain reaction","response elements","signal transduction","transcription factors/*genetics/metabolism"],"search_terms":["cytokinin","response","factors","regulate","pin","formed","auxin","transporters","simaskova","o'brien","khan","van noorden","otvos","vieten","de clercq","van haperen","cuesta","hoyerova","vanneste","marhavý","wabnik","van breusegem","nowack","murphy","friml","weijers","beeckman","benkova"],"title":"Cytokinin response factors regulate PIN-FORMED auxin transporters","year":2015}