Improved methodology for monitoring poly(amidoamine) dendrimers surface transformations and product quality by ultra performance liquid chromatography. a. Cason, C., a. Oehrle, S., Fabre, A, T., Girten, C. D., a. Walters, K., a. Tomalia, D., Haik, K. L., a. Bullen, H., & a. Fabré, T. Journal of Nanomaterials, 2008:No pp. given, Hindawi Publishing Corp., 2008.
Improved methodology for monitoring poly(amidoamine) dendrimers surface transformations and product quality by ultra performance liquid chromatography. [link]Paper  doi  abstract   bibtex   
Ultra performance liq. chromatog. (UPLC) anal. was utilized for the first time as a methodol. for monitoring poly(amidoamine) (PAMAM) dendrimer surface transformations and product quality. Results were compared to high-performance liq. chromatog. (HPLC) and were found to provide a vastly improved anal. method for the characterization of dendrimer polydispersity and variance in a typical surface modification. The application of UPLC increased the av. no. of theor. plates by a factor of 7 and reduced retention times of analytes by 36%, while improving the resoln. capability to discriminate surface variances in dendrimers. The new UPLC procedures were used to monitor surface modification of [core: ethylenediamine]; (G = 4); dendri-poly(amidoamine)-(NH2)64 (i.e., [EDA]; (G4); dendri-PAMAM-(NH2)64) to produce biotinylated dendrimer conjugates. The enhanced sensitivity and efficiency of the UPLC analyses allowed resoln. of biotin substituent levels and a better characterization of the targeted dendrimer conjugates compared to traditional HPLC methodol. [on SciFinder(R)]
@article{ Cason2008,
  abstract = {Ultra performance liq. chromatog. (UPLC) anal. was utilized for the first time as a methodol. for monitoring poly(amidoamine) (PAMAM) dendrimer surface transformations and product quality.  Results were compared to high-performance liq. chromatog. (HPLC) and were found to provide a vastly improved anal. method for the characterization of dendrimer polydispersity and variance in a typical surface modification.  The application of UPLC increased the av. no. of theor. plates by a factor of 7 and reduced retention times of analytes by 36%, while improving the resoln. capability to discriminate surface variances in dendrimers.  The new UPLC procedures were used to monitor surface modification of [core: ethylenediamine]; (G = 4); dendri-poly(amidoamine)-(NH2)64 (i.e., [EDA]; (G4); dendri-PAMAM-(NH2)64) to produce biotinylated dendrimer conjugates.  The enhanced sensitivity and efficiency of the UPLC analyses allowed resoln. of biotin substituent levels and a better characterization of the targeted dendrimer conjugates compared to traditional HPLC methodol. [on SciFinder(R)]},
  annote = {From Duplicate 2 ( },
  author = {Cason, Chevelle a. and Oehrle, Stuart a. and Fabre, Thomas A and Girten, Craig D. and Walters, Keith a. and Tomalia, Donald a. and Haik, Kristi L. and Bullen, Heather a. and Fabré, Thomas a.},
  doi = {10.1155/2008/456082},
  file = {:C$\backslash$:/Users/walterske/SkyDrive/References/Journal of Nanomaterials/2008 - Cason et al. - Improved methodology for monitoring poly(amidoamine) dendrimers surface transformations and product quality by ult.pdf:pdf},
  issn = {1687-4129},
  journal = {Journal of Nanomaterials},
  keywords = {poly amidoamine dendrimer surface transformation p,ultra performance liq chromatog},
  pages = {No pp. given},
  publisher = {Hindawi Publishing Corp.},
  title = {{Improved methodology for monitoring poly(amidoamine) dendrimers surface transformations and product quality by ultra performance liquid chromatography.}},
  url = {http://www.hindawi.com/journals/jnm/2008/456082/ http://www.hindawi.com/GetArticle.aspx?doi=10.1155/2008/456082},
  volume = {2008},
  year = {2008}
}

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