{"_id":"CEtxEx8RiQbciiXo9","bibbaseid":"anonymous-cdc2phosphorylationofnucleolindemarcatesmitoticstagesandalzheimersdiseasepathology-2001","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Dranovsky"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Vincent"],"firstnames":["I."],"suffixes":[]},{"propositions":[],"lastnames":["Gregori"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Schwarzman"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Colflesh"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Enghild"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Strittmatter"],"firstnames":["W."],"suffixes":[]},{"propositions":[],"lastnames":["Davies"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Goldgaber"],"firstnames":["D."],"suffixes":[]}],"title":"Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology","journal":"Neurobiology of Aging","year":"2001","volume":"22","number":"4","pages":"517-528","doi":"10.1016/S0197-4580(00)00248-7","note":"cited By 71","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034972923&doi=10.1016%2fS0197-4580%2800%2900248-7&partnerID=40&md5=e29e13de255db974d4682dc68620aca9","affiliation":"Medical Scientist Training Program, State University of New York, Stony Brook, NY 11794, United States; Department of Psychiatry and Behavioral Science, State University of New York, Stony Brook, NY 11794, United States; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, United States; University Microscopy Imaging Center, State University of New York, Stony Brook, NY 11794, United States; Department of Neurology, Duke University, Durham, NC 27710, United States","abstract":"Nucleolin is a major multifunctional nuclear phosphoprotein that is phosphorylated by Cdc2 kinase in mitosis and that participates in a number of cellular processes. The monoclonal antibody TG-3 generated against neurofibrillary tangles (NFT) found in Alzheimer's disease (AD) is highly specific for mitotic cells in culture. We here demonstrate that phosphorylation of nucleolin by Cdc2 kinase generates the TG-3 epitope. The unique pool of TG-3 immunoreactive nucleolin appears abruptly during the prophase. It is associated with chromosomes through the metaphase and it gradually disappears during separation of chromosomes and exit from mitosis. In the brain, nucleolin was localized not only to nuclei but also to neuronal cytoplasm, and it is a marker for early NFT. In patients with AD, Cdc2 phosphorylated nucleolin was present in NFT. These findings suggest that phosphorylation of nucleolin by Cdc2 kinase is a critical event and the point of convergence of two distinct pathways, mitosis and neurodegeneration. © 2001 Elsevier Science Inc.","author_keywords":"Alzheimer's disease; Mitosis; Neurodegeneration; Nucleolin","funding_details":"AG/OD 12721","key":"Dranovsky2001517","id":"Dranovsky2001517","bibbaseid":"anonymous-cdc2phosphorylationofnucleolindemarcatesmitoticstagesandalzheimersdiseasepathology-2001","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034972923&doi=10.1016%2fS0197-4580%2800%2900248-7&partnerID=40&md5=e29e13de255db974d4682dc68620aca9"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lmgch_2019_10.txt","dataSources":["87fXC3NAxFNMB5YTH"],"keywords":[],"search_terms":["cdc2","phosphorylation","nucleolin","demarcates","mitotic","stages","alzheimer","disease","pathology"],"title":"Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology","year":2001}