Zipping up transcription factors: Rational design of anti-Jun and anti-Fos peptides. Bains, N., P., S., Wilce, J., A., Heuer, K., H., Tunstall, M., Mackey, J., P., Bennett, M., R., Weiss, A., S., & King, G., F. Letters in Peptide Science, 4(2):67-77, 4, 1997.
Zipping up transcription factors: Rational design of anti-Jun and anti-Fos peptides [link]Website  abstract   bibtex   
Various members of the bZip and bHLH-Zip families of eukaryotic transcription factors, including Jun, Fos, and Myc, have been identified as oncoproteins; mutation or deregulated expression of these proteins leads to certain types of cancer. These proteins can only bind to their cognate DNA enhancer sites following homodimerization, or heterodimerization with another family member, via their leucine zipper domain. Thus, a novel anticancer strategy would be to inhibit dimerization of these proteins, thereby blocking their DNA binding and transactivation functions. In this paper we show that it is possible to rationally design leucine zipper peptides that bind with high affinity to the leucine zipper dimerization domains of c-Jun and c-Fos, thus preventing the formation of functional c-Jun homodimers and c-Jun:c-Fos heterodimers; we refer to such peptides as superzippers (SZs). In vivo, c-Jun:SZ and c-Fos:SZ heterodimers should be nonfunctional as they lack one of the two basic domains that are essential for DNA binding. While the transport of a peptidic agent into cells often poses a severe obstacle to its therapeutic use, we show that a 46-residue leucine zipper peptide can be transported into HeLa cells by coupling it to a 17-residue carrier peptide from the Antennapedia homeodomain, thus paving the way for detailed studies of the therapeutic potential of superzipper peptides. © 1997 ESCOM Science Publishers B.V.
@article{
 title = {Zipping up transcription factors: Rational design of anti-Jun and anti-Fos peptides},
 type = {article},
 year = {1997},
 identifiers = {[object Object]},
 keywords = {Anticancer drugs,Fos,Jun,Leucine zipper,Peptide delivery,Peptide drug,Transcription factors},
 pages = {67-77},
 volume = {4},
 websites = {http://link.springer.com/10.1007/BF02443517},
 month = {4},
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 abstract = {Various members of the bZip and bHLH-Zip families of eukaryotic transcription factors, including Jun, Fos, and Myc, have been identified as oncoproteins; mutation or deregulated expression of these proteins leads to certain types of cancer. These proteins can only bind to their cognate DNA enhancer sites following homodimerization, or heterodimerization with another family member, via their leucine zipper domain. Thus, a novel anticancer strategy would be to inhibit dimerization of these proteins, thereby blocking their DNA binding and transactivation functions. In this paper we show that it is possible to rationally design leucine zipper peptides that bind with high affinity to the leucine zipper dimerization domains of c-Jun and c-Fos, thus preventing the formation of functional c-Jun homodimers and c-Jun:c-Fos heterodimers; we refer to such peptides as superzippers (SZs). In vivo, c-Jun:SZ and c-Fos:SZ heterodimers should be nonfunctional as they lack one of the two basic domains that are essential for DNA binding. While the transport of a peptidic agent into cells often poses a severe obstacle to its therapeutic use, we show that a 46-residue leucine zipper peptide can be transported into HeLa cells by coupling it to a 17-residue carrier peptide from the Antennapedia homeodomain, thus paving the way for detailed studies of the therapeutic potential of superzipper peptides. © 1997 ESCOM Science Publishers B.V.},
 bibtype = {article},
 author = {Bains, Naresh P. S. and Wilce, Jackie A. and Heuer, Katja H. and Tunstall, Mark and Mackey, Joel P. and Bennett, Max R. and Weiss, Anthony S. and King, Glenn F.},
 journal = {Letters in Peptide Science},
 number = {2}
}

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