Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit. Casanova-Sáez, R., Mateo-Bonmatí, E., Šimura, J., Pěnčík, A., Novák, O., Staswick, P., & Ljung, K. New Phytologist, March, 2022.
Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit [link]Paper  doi  abstract   bibtex   
Indole-3-acetic acid (IAA) controls a plethora of developmental processes. Thus, regulation of their levels is of great relevance for plant performance. Cellular IAA concentration is a result of its transport, biosynthesis and various pathways for IAA inactivation, including oxidation and conjugation. Group II members of the GRETCHEN HAGEN 3 (GH3) gene family code for acyl acid amido synthetases catalysing the conjugation of IAA to amino acids. However, the high level of functional redundancy among them has hampered thorough analysis of their roles in plant development. In this work, we generated an Arabidopsis gh3.1,2,3,4,5,6,9,17 (gh3oct) mutant to knock out the group II GH3 pathway. The gh3oct plants had an elaborated root architecture, were more tolerant to osmotic stresses due to locally increased IAA levels and showed increased tolerance to water deficit. IAA metabolite quantification in gh3oct plants suggested the existence of additional GH3-like enzymes in IAA metabolism. Moreover, our data suggested that oxIAA production depends, at least partly, on the GH3 pathway. Targeted stress-hormone analysis further suggested involvement of ABA in the differential response to salinity of gh3oct plants. Taken together, our data provide new insights into the roles of group II GH3s in IAA metabolism and hormone-regulated plant development.
@article{casanova-saez_inactivation_2022,
	title = {Inactivation of the entire {Arabidopsis} group {II} {GH3s} confers tolerance to salinity and water deficit},
	issn = {1469-8137},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/nph.18114},
	doi = {10.1111/nph.18114},
	abstract = {Indole-3-acetic acid (IAA) controls a plethora of developmental processes. Thus, regulation of their levels is of great relevance for plant performance. Cellular IAA concentration is a result of its transport, biosynthesis and various pathways for IAA inactivation, including oxidation and conjugation. Group II members of the GRETCHEN HAGEN 3 (GH3) gene family code for acyl acid amido synthetases catalysing the conjugation of IAA to amino acids. However, the high level of functional redundancy among them has hampered thorough analysis of their roles in plant development. In this work, we generated an Arabidopsis gh3.1,2,3,4,5,6,9,17 (gh3oct) mutant to knock out the group II GH3 pathway. The gh3oct plants had an elaborated root architecture, were more tolerant to osmotic stresses due to locally increased IAA levels and showed increased tolerance to water deficit. IAA metabolite quantification in gh3oct plants suggested the existence of additional GH3-like enzymes in IAA metabolism. Moreover, our data suggested that oxIAA production depends, at least partly, on the GH3 pathway. Targeted stress-hormone analysis further suggested involvement of ABA in the differential response to salinity of gh3oct plants. Taken together, our data provide new insights into the roles of group II GH3s in IAA metabolism and hormone-regulated plant development.},
	language = {en},
	urldate = {2022-03-24},
	journal = {New Phytologist},
	author = {Casanova-Sáez, Rubén and Mateo-Bonmatí, Eduardo and Šimura, Jan and Pěnčík, Aleš and Novák, Ondřej and Staswick, Paul and Ljung, Karin},
	month = mar,
	year = {2022},
	keywords = {Arabidopsis, GH3, auxin, drought, salinity, stress tolerance},
}

Downloads: 0