Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli

Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli. Maric, S.; Thygesen, M. B.; Schiller, J.; Marek, M.; Moulin, M.; Haertlein, M.; Forsyth, V. T.; Bogdanov, M.; Dowhan, W.; Arleth, L.; and Pomorski, T. G. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(1, SI):241-254, JAN, 2015.
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Phosphatidylcholine (PC) is a major component of eukaryotic cell membranes and one of the most commonly used phospholipids for reconstitution of membrane proteins into carrier systems such as lipid vesicles, micelles and nanodiscs. Selectively deuterated versions of this lipid have many applications, especially in structural studies using techniques such as NMR, neutron reflectivity and small-angle neutron scattering. Here we present a comprehensive study of selective deuteration of phosphatidylcholine through biosynthesis in a genetically modified strain of Escherichia coli. By carefully tuning the deuteration level in E. coli growth media and varying the deuteration of supplemented carbon sources, we show that it is possible to achieve a controlled deuteration for three distinct parts of the PC lipid molecule, namely the (a) lipid head group, (b) glycerol backbone and (c) fatty acyl tail. This biosynthetic approach paves the way for the synthesis of specifically deuterated, physiologically relevant phospholipid species which remain difficult to obtain through standard chemical synthesis.

@article{ISI:000347685300023,
	Abstract = {{Phosphatidylcholine (PC) is a major component of eukaryotic cell
   membranes and one of the most commonly used phospholipids for
   reconstitution of membrane proteins into carrier systems such as lipid
   vesicles, micelles and nanodiscs. Selectively deuterated versions of
   this lipid have many applications, especially in structural studies
   using techniques such as NMR, neutron reflectivity and small-angle
   neutron scattering. Here we present a comprehensive study of selective
   deuteration of phosphatidylcholine through biosynthesis in a genetically
   modified strain of Escherichia coli. By carefully tuning the deuteration
   level in E. coli growth media and varying the deuteration of
   supplemented carbon sources, we show that it is possible to achieve a
   controlled deuteration for three distinct parts of the PC lipid
   molecule, namely the (a) lipid head group, (b) glycerol backbone and (c)
   fatty acyl tail. This biosynthetic approach paves the way for the
   synthesis of specifically deuterated, physiologically relevant
   phospholipid species which remain difficult to obtain through standard
   chemical synthesis.}},
	Author = {Maric, Selma and Thygesen, Mikkel B. and Schiller, Juergen and Marek, Magdalena and Moulin, Martine and Haertlein, Michael and Forsyth, V. Trevor and Bogdanov, Mikhail and Dowhan, William and Arleth, Lise and Pomorski, Thomas Guenther},
	Date-Added = {2017-10-03 14:11:19 +0000},
	Date-Modified = {2017-10-03 14:11:19 +0000},
	Doi = {{10.1007/s00253-014-6082-z}},
	Eissn = {{1432-0614}},
	Issn = {{0175-7598}},
	Journal = {{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}},
	Month = {{JAN}},
	Number = {{1, SI}},
	Orcid-Numbers = {{Forsyth, V. Trevor/0000-0003-0380-3477 Gunther Pomorski, Thomas/0000-0002-4889-0829 Thygesen, Mikkel/0000-0002-0158-2802 Arleth, Lise/0000-0002-4694-4299}},
	Pages = {{241-254}},
	Researcherid-Numbers = {{Forsyth, V. Trevor/A-9129-2010 Gunther Pomorski, Thomas/G-4804-2014 Thygesen, Mikkel/D-1098-2016 Bogdanov, Mikhail/J-3695-2017 }},
	Title = {{Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli}},
	Unique-Id = {{ISI:000347685300023}},
	Volume = {{99}},
	Year = {{2015}},
	Bdsk-Url-1 = {http://dx.doi.org/10.1007/s00253-014-6082-z%7D}}

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