Neuropeptides myomodulin, small cardioactive peptide, and buccalin in the central nervous system ofLymnaea stagnalis: Purification, immunoreactivity, and artifacts. Santama, N.; Wheeler, C. H.; Burke, J. F.; and Benjamin, P. R. The Journal of Comparative Neurology, 342(3):335–351, Wiley Online Library, apr, 1994.
Neuropeptides myomodulin, small cardioactive peptide, and buccalin in the central nervous system ofLymnaea stagnalis: Purification, immunoreactivity, and artifacts [link]Paper  doi  abstract   bibtex   
The neuropeptides myomodulin, small cardioactive peptide (SCP), and buccalin are widely distributed in the phylum Mollusca and have important physiological functions. Here, we describe the detailed distribution of each class of peptide in the central nervous system (CNS) of the snail Lymnaea stagnalis by the use of immunocytochemical techniques combined with dye‐marking of electrophysiologically identified neurons. We report the isolation and structural characterization of a Lymnaea myomodulin, PMSMLRLamide, identical to myomodulin A of Aplysia californica. Myomodulin immunoreactivity was localized in all 11 ganglia, in their connectives, and in peripheral nerves. In many cases, myomodulin immunoreactivity appeared localized in neuronal clusters expressing FMRFamide‐like peptides, but also in a large number of additional neurons. Double‐labelling experiments demonstrated myomodulin immunoreactivity in the visceral white interneuron, involved in regulation of cardiorespiration. SCP‐like immunoreactivity also appeared in all ganglia, and double‐labelling experiments revealed that in many locations it was specifically associated with clusters expressing distinct exons of the FMRFamide gene that are differentially expressed in the CNS. Characterization of the two types of SCP‐antisera used in this study, however, suggested that they cross‐reacted with both FMRFamide and N‐terminally extended FMRFamide‐like peptides. Selective preadsorption with these cross‐reacting peptides resulted in elimination of the widespread staining and retention of bona fide SCP immunoreactivity in the buccal and pedal ganglia only. Buccalin immunoreactivity was limited to the buccal and pedal ganglia. It did not coincide with the distribution of either myomodulin or SCP. Most immunoreactive clusters were found in the pedal ganglia. \textcopyright 1994 Wiley‐Liss, Inc. Copyright \textcopyright 1994 Wiley‐Liss, Inc.
@article{pop00117,
abstract = {The neuropeptides myomodulin, small cardioactive peptide (SCP), and buccalin are widely distributed in the phylum Mollusca and have important physiological functions. Here, we describe the detailed distribution of each class of peptide in the central nervous system (CNS) of the snail Lymnaea stagnalis by the use of immunocytochemical techniques combined with dye‐marking of electrophysiologically identified neurons. We report the isolation and structural characterization of a Lymnaea myomodulin, PMSMLRLamide, identical to myomodulin A of Aplysia californica. Myomodulin immunoreactivity was localized in all 11 ganglia, in their connectives, and in peripheral nerves. In many cases, myomodulin immunoreactivity appeared localized in neuronal clusters expressing FMRFamide‐like peptides, but also in a large number of additional neurons. Double‐labelling experiments demonstrated myomodulin immunoreactivity in the visceral white interneuron, involved in regulation of cardiorespiration. SCP‐like immunoreactivity also appeared in all ganglia, and double‐labelling experiments revealed that in many locations it was specifically associated with clusters expressing distinct exons of the FMRFamide gene that are differentially expressed in the CNS. Characterization of the two types of SCP‐antisera used in this study, however, suggested that they cross‐reacted with both FMRFamide and N‐terminally extended FMRFamide‐like peptides. Selective preadsorption with these cross‐reacting peptides resulted in elimination of the widespread staining and retention of bona fide SCP immunoreactivity in the buccal and pedal ganglia only. Buccalin immunoreactivity was limited to the buccal and pedal ganglia. It did not coincide with the distribution of either myomodulin or SCP. Most immunoreactive clusters were found in the pedal ganglia. {\textcopyright} 1994 Wiley‐Liss, Inc. Copyright {\textcopyright} 1994 Wiley‐Liss, Inc.},
annote = {Query date: 2020-06-29 13:05:30},
author = {Santama, Niovi and Wheeler, Colin H. and Burke, Julian F. and Benjamin, Paul R.},
doi = {10.1002/cne.903420303},
issn = {0021-9967},
journal = {The Journal of Comparative Neurology},
keywords = {FMRFamide,colocalization,immunocytochemistry,molluscs,peptidergic neurons},
month = {apr},
number = {3},
pages = {335--351},
publisher = {Wiley Online Library},
title = {{Neuropeptides myomodulin, small cardioactive peptide, and buccalin in the central nervous system ofLymnaea stagnalis: Purification, immunoreactivity, and artifacts}},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.903420303 http://doi.wiley.com/10.1002/cne.903420303},
volume = {342},
year = {1994}
}
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