Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro. Syed, N. I. & Spencer, G. E. Netherlands Journal of Zoology, 44(3-4):327–338, brill.com, 1993.
Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro [link]Paper  doi  abstract   bibtex   
Our research is aimed at determining both cellular and molecular mechanisms that underlie target cell selection and specific synapse formation in the nervous system. We have developed an in vitro experimental system which utilizes identified pre and postsynaptic neurons from the mollusc Iymnaea stagnalis. A giant presynaptic neuron, the right pedal dorsal 1 (RPeDl), re-establishes its specific synapses in culture with only postsynaptic target cells (such as visceral J, I), but not with non target cells (VF, RPB). In an attempt to begin to elucidate mechanisms that determine the specificity of synap- togenesis between Lymnaea neurons in vitro, we studied the behaviour of both target and non-target cell growth cones as they encountered RPcDl's growth cones for the first time. Utilizing time-lapse video imaging techniques, we demonstrate that RPeDl growth cones attract and repel target and non-target cell growth concs respectively. We suggest that the specificity of synaptogenesis between RPeDl and both target and nontarget cells is determined at the growth conc level and may involve release of a diffusible substance from the growth concs of RPeDl. \textcopyright 1993, BRILL
@article{pop00495,
abstract = {Our research is aimed at determining both cellular and molecular mechanisms that underlie target cell selection and specific synapse formation in the nervous system. We have developed an in vitro experimental system which utilizes identified pre and postsynaptic neurons from the mollusc Iymnaea stagnalis. A giant presynaptic neuron, the right pedal dorsal 1 (RPeDl), re-establishes its specific synapses in culture with only postsynaptic target cells (such as visceral J, I), but not with non target cells (VF, RPB). In an attempt to begin to elucidate mechanisms that determine the specificity of synap- togenesis between Lymnaea neurons in vitro, we studied the behaviour of both target and non-target cell growth cones as they encountered RPcDl's growth cones for the first time. Utilizing time-lapse video imaging techniques, we demonstrate that RPeDl growth cones attract and repel target and non-target cell growth concs respectively. We suggest that the specificity of synaptogenesis between RPeDl and both target and nontarget cells is determined at the growth conc level and may involve release of a diffusible substance from the growth concs of RPeDl. {\textcopyright} 1993, BRILL},
annote = {Query date: 2020-06-29 13:05:30},
author = {Syed, Naweed I. and Spencer, Gaynor E.},
doi = {10.1163/156854293X00430},
issn = {00282960},
journal = {Netherlands Journal of Zoology},
keywords = {Lymnaea,dopamine,growth concs,identified neurons,in vitro,neurite outgrowth,synapse formation,target selection},
number = {3-4},
pages = {327--338},
publisher = {brill.com},
title = {{Target Cell Selection and Specific Synapse Formation By Identified Ly'Mnaea Neurons in Vitro}},
url = {https://brill.com/view/journals/njz/44/3-4/article-p327{\_}14.xml},
volume = {44},
year = {1993}
}

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