Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases. Vellaichamy, A., Sreekumar, A., Strahler, J. R., Rajendiran, T., Yu, J., Varambally, S., Li, Y., Omenn, G. S., Chinnaiyan, A. M., & Nesvizhskii, A. I. PLOS ONE, SEP 18, 2009.
doi  abstract   bibtex   
Prostate cancer remains the most common malignancy among men in United States, and there is no remedy currently available for the advanced stage hormone-refractory cancer. This is partly due to the incomplete understanding of androgen-regulated proteins and their encoded functions. Whole-cell proteomes of androgen-starved and androgen-treated LNCaP cells were analyzed by semi-quantitative MudPIT ESI-ion trap MS/MS and quantitative iTRAQ MALDI-TOF MS/MS platforms, with identification of more than 1300 high-confidence proteins. An enrichment-based pathway mapping of the androgen-regulated proteomic data sets revealed a significant dysregulation of aminoacyl tRNA synthetases, indicating an increase in protein biosynthesis-a hallmark during prostate cancer progression. This observation is supported by immunoblot and transcript data from LNCaP cells, and prostate cancer tissue. Thus, data derived from multiple proteomics platforms and transcript data coupled with informatics analysis provides a deeper insight into the functional consequences of androgen action in prostate cancer.
@article{ ISI:000269970400011,
Author = {Vellaichamy, Adaikkalam and Sreekumar, Arun and Strahler, John R. and
   Rajendiran, Theckelnaycke and Yu, Jindan and Varambally, Sooryanarayana
   and Li, Yong and Omenn, Gilbert S. and Chinnaiyan, Arul M. and
   Nesvizhskii, Alexey I.},
Title = {{Proteomic Interrogation of Androgen Action in Prostate Cancer Cells
   Reveals Roles of Aminoacyl tRNA Synthetases}},
Journal = {{PLOS ONE}},
Year = {{2009}},
Volume = {{4}},
Number = {{9}},
Month = {{SEP 18}},
Abstract = {{Prostate cancer remains the most common malignancy among men in United
   States, and there is no remedy currently available for the advanced
   stage hormone-refractory cancer. This is partly due to the incomplete
   understanding of androgen-regulated proteins and their encoded
   functions. Whole-cell proteomes of androgen-starved and androgen-treated
   LNCaP cells were analyzed by semi-quantitative MudPIT ESI-ion trap MS/MS
   and quantitative iTRAQ MALDI-TOF MS/MS platforms, with identification of
   more than 1300 high-confidence proteins. An enrichment-based pathway
   mapping of the androgen-regulated proteomic data sets revealed a
   significant dysregulation of aminoacyl tRNA synthetases, indicating an
   increase in protein biosynthesis-a hallmark during prostate cancer
   progression. This observation is supported by immunoblot and transcript
   data from LNCaP cells, and prostate cancer tissue. Thus, data derived
   from multiple proteomics platforms and transcript data coupled with
   informatics analysis provides a deeper insight into the functional
   consequences of androgen action in prostate cancer.}},
DOI = {{10.1371/journal.pone.0007075}},
Article-Number = {{e7075}},
ISSN = {{1932-6203}},
ResearcherID-Numbers = {{Nesvizhskii, Alexey/A-5410-2012}},
Unique-ID = {{ISI:000269970400011}},
}

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