@article{yew_cuticular_2008,
	title = {Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry},
	volume = {105},
	url = {internal-pdf://PNAS_2008_105_7135-7140-2041145631/PNAS_2008_105_7135-7140.pdf},
	doi = {10.1073/pnas.0802692105},
	abstract = {In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In , pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study cuticular hydrocarbons. Although GC/MS is highly reproducible and sensitive, it requires that the fly be placed in a lethal solution of organic solvent, thereby impeding further behavioral studies. We present a technique for the analysis of hydrocarbons and other surface molecules from live animals by using direct analysis in real-time (DART) MS. Cuticular hydrocarbons were sampled from the surface of a restrained, awake behaving fly by using several brief, carefully controlled depressions of the abdomen with a small steel probe. DART mass spectral analysis of the probe detected ions with mass-to-charge ratio (/) of the protonated molecule corresponding to many of the previously identified unsaturated hydrocarbons. Six additional cuticular hydrocarbons also were identified. Consistent with previous GC/MS studies, male and female differences in chemical composition were evident. Spatial differences in the expression profile also were observed on males. Sampling from an individual female first as a virgin and then 45 and 90 min after successful copulation showed that mass signals likely to correspond to -vaccenyl acetate, tricosene, and pentacosene increased in relative intensity after courtship. This method provides near-instantaneous analysis of an individual animal's chemical profile in parallel with behavioral studies and could be extended to other models of pheromone-mediated behavior.},
	number = {20},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Yew, Joanne Y. and Cody, Robert B. and Kravitz, Edward A.},
	year = {2008},
	keywords = {AccuTOF},
	pages = {7135--7140},
}

{"_id":"tdqG4RYNLXZzQepsF","authorIDs":[],"author_short":["Yew, J. Y.","Cody, R. B.","Kravitz, E. A."],"bibbaseid":"yew-cody-kravitz-cuticularhydrocarbonanalysisofanawakebehavingflyusingdirectanalysisinrealtimetimeofflightmassspectrometry-2008","bibdata":{"bibtype":"article","type":"article","title":"Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry","volume":"105","url":"internal-pdf://PNAS_2008_105_7135-7140-2041145631/PNAS_2008_105_7135-7140.pdf","doi":"10.1073/pnas.0802692105","abstract":"In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In , pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study cuticular hydrocarbons. Although GC/MS is highly reproducible and sensitive, it requires that the fly be placed in a lethal solution of organic solvent, thereby impeding further behavioral studies. We present a technique for the analysis of hydrocarbons and other surface molecules from live animals by using direct analysis in real-time (DART) MS. Cuticular hydrocarbons were sampled from the surface of a restrained, awake behaving fly by using several brief, carefully controlled depressions of the abdomen with a small steel probe. DART mass spectral analysis of the probe detected ions with mass-to-charge ratio (/) of the protonated molecule corresponding to many of the previously identified unsaturated hydrocarbons. Six additional cuticular hydrocarbons also were identified. Consistent with previous GC/MS studies, male and female differences in chemical composition were evident. Spatial differences in the expression profile also were observed on males. Sampling from an individual female first as a virgin and then 45 and 90 min after successful copulation showed that mass signals likely to correspond to -vaccenyl acetate, tricosene, and pentacosene increased in relative intensity after courtship. This method provides near-instantaneous analysis of an individual animal's chemical profile in parallel with behavioral studies and could be extended to other models of pheromone-mediated behavior.","number":"20","journal":"Proceedings of the National Academy of Sciences","author":[{"propositions":[],"lastnames":["Yew"],"firstnames":["Joanne","Y."],"suffixes":[]},{"propositions":[],"lastnames":["Cody"],"firstnames":["Robert","B."],"suffixes":[]},{"propositions":[],"lastnames":["Kravitz"],"firstnames":["Edward","A."],"suffixes":[]}],"year":"2008","keywords":"AccuTOF","pages":"7135–7140","bibtex":"@article{yew_cuticular_2008,\n\ttitle = {Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry},\n\tvolume = {105},\n\turl = {internal-pdf://PNAS_2008_105_7135-7140-2041145631/PNAS_2008_105_7135-7140.pdf},\n\tdoi = {10.1073/pnas.0802692105},\n\tabstract = {In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In , pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study cuticular hydrocarbons. Although GC/MS is highly reproducible and sensitive, it requires that the fly be placed in a lethal solution of organic solvent, thereby impeding further behavioral studies. We present a technique for the analysis of hydrocarbons and other surface molecules from live animals by using direct analysis in real-time (DART) MS. Cuticular hydrocarbons were sampled from the surface of a restrained, awake behaving fly by using several brief, carefully controlled depressions of the abdomen with a small steel probe. DART mass spectral analysis of the probe detected ions with mass-to-charge ratio (/) of the protonated molecule corresponding to many of the previously identified unsaturated hydrocarbons. Six additional cuticular hydrocarbons also were identified. Consistent with previous GC/MS studies, male and female differences in chemical composition were evident. Spatial differences in the expression profile also were observed on males. Sampling from an individual female first as a virgin and then 45 and 90 min after successful copulation showed that mass signals likely to correspond to -vaccenyl acetate, tricosene, and pentacosene increased in relative intensity after courtship. This method provides near-instantaneous analysis of an individual animal's chemical profile in parallel with behavioral studies and could be extended to other models of pheromone-mediated behavior.},\n\tnumber = {20},\n\tjournal = {Proceedings of the National Academy of Sciences},\n\tauthor = {Yew, Joanne Y. and Cody, Robert B. and Kravitz, Edward A.},\n\tyear = {2008},\n\tkeywords = {AccuTOF},\n\tpages = {7135--7140},\n}\n\n","author_short":["Yew, J. Y.","Cody, R. B.","Kravitz, E. 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