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\n\n \n \n \n \n \n \n Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy.\n \n \n \n \n\n\n \n Omar, M.; Rebling, J.; Wicker, K.; Schmitt-Manderbach, T.; Schwarz, M.; Gateau, J.; López-Schier, H.; Mappes, T.; and Ntziachristos, V.\n\n\n \n\n\n\n
Light: Science and Applications, 6(1): e16186-e16186. 1 2017.\n
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@article{\n title = {Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy},\n type = {article},\n year = {2017},\n keywords = {deconvolution,development,mesoscopy,multiview,optoacoustics,photoacoustics},\n pages = {e16186-e16186},\n volume = {6},\n websites = {http://www.nature.com/articles/lsa2016186},\n month = {1},\n day = {11},\n id = {16ecc034-a2f0-3fff-9656-556cefcfe4ee},\n created = {2020-07-11T11:23:46.025Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n group_id = {9be6478b-54cd-3713-9237-f1e3cf6da833},\n last_modified = {2020-07-18T08:43:14.270Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n citation_key = {Omar2017},\n private_publication = {false},\n abstract = {Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.},\n bibtype = {article},\n author = {Omar, Murad and Rebling, Johannes and Wicker, Kai and Schmitt-Manderbach, Tobias and Schwarz, Mathias and Gateau, Jérôme and López-Schier, Hérnan and Mappes, Timo and Ntziachristos, Vasilis},\n doi = {10.1038/lsa.2016.186},\n journal = {Light: Science and Applications},\n number = {1}\n}
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\n Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.\n
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