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\n\n \n \n 2020\n \n \n (4)\n \n \n
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\n \n \n\n \n \n \n \n \n Rapid functional optoacoustic micro-angiography in a burst mode.\n \n \n \n\n\n \n Hofmann, U., A., T.; Rebling, J.; Estrada, H.; Subochev, P.; and Razansky, D.\n\n\n \n\n\n\n Optics Letters, 45(9): 2522. 2020.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
\n\n\n \n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Rapid functional optoacoustic micro-angiography in a burst mode},\n type = {article},\n year = {2020},\n pages = {2522},\n volume = {45},\n id = {9a33a53d-9e08-31d9-ae0a-ba72f92c86ac},\n created = {2020-07-11T11:23:47.492Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.492Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Optoacoustic microscopy (OAM) can image intrinsic optical absorption contrast at depths of several millimeters where state-of-the-art optical microscopy techniques fail due to intense light scattering in living tissues. Yet, wide adoption of OAM in biology and medicine is hindered by slow image acquisition speed, small field of view (FOV), and/or lack of spectral differentiation capacity of common system implementations. We report on a rapid acquisition functional optoacoustic micro-angiography approach that employs a burst-mode laser triggering scheme to simultaneously acquire multi-wavelength 3D images over an extended FOV covering $50\\;\\rm mm \\times 50\\;\\rm mm$50mm×50mm in a single mechanical overfly scan, attaining 28 µm and 14 µm resolution in lateral and axial dimensions, respectively. Owing to an ultrawideband low-noise design featuring a spherically focused polyvinylidene difluoride transducer, we demonstrate imaging of human skin and underlying vasculature at up to 3.8 mm depth when using per-pulse laser energies of only 25 µJ without employing signal averaging. Overall, the developed system greatly enhances performance and usability of OAM for dermatologic and micro-angiographic studies.},\n bibtype = {article},\n author = {Hofmann, Urs A. T. and Rebling, Johannes and Estrada, Héctor and Subochev, Pavel and Razansky, Daniel},\n doi = {10.1364/ol.387630},\n journal = {Optics Letters},\n number = {9}\n}\n\n Optoacoustic microscopy (OAM) can image intrinsic optical absorption contrast at depths of several millimeters where state-of-the-art optical microscopy techniques fail due to intense light scattering in living tissues. Yet, wide adoption of OAM in biology and medicine is hindered by slow image acquisition speed, small field of view (FOV), and/or lack of spectral differentiation capacity of common system implementations. We report on a rapid acquisition functional optoacoustic micro-angiography approach that employs a burst-mode laser triggering scheme to simultaneously acquire multi-wavelength 3D images over an extended FOV covering $50\\;\\rm mm \\times 50\\;\\rm mm$50mm×50mm in a single mechanical overfly scan, attaining 28 µm and 14 µm resolution in lateral and axial dimensions, respectively. Owing to an ultrawideband low-noise design featuring a spherically focused polyvinylidene difluoride transducer, we demonstrate imaging of human skin and underlying vasculature at up to 3.8 mm depth when using per-pulse laser energies of only 25 µJ without employing signal averaging. Overall, the developed system greatly enhances performance and usability of OAM for dermatologic and micro-angiographic studies.\n
\n\n\n\n \n\n \n \n \n \n \n \n Intravital optoacoustic and ultrasound bio-microscopy reveal radiation-inhibited skull angiogenesis.\n \n \n \n \n\n\n \n Estrada, H.; Rebling, J.; Sievert, W.; Hladik, D.; Hofmann, U.; Gottschalk, S.; Tapio, S.; Multhoff, G.; and Razansky, D.\n\n\n \n\n\n\n Bone, 133(October 2019). 2020.\n \n\n\n\n
\n\n\n\n \n \n![\"Intravital](\"//bibbase.org/img/filetypes/pdf.svg\"\n\t "\\"Paper\\"\n\t")
Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n\n
\n\n\n@article{\n title = {Intravital optoacoustic and ultrasound bio-microscopy reveal radiation-inhibited skull angiogenesis},\n type = {article},\n year = {2020},\n keywords = {Bone angiogenesis,Image segmentation,Optoacoustic microscopy,Quantitative vasculature analysis,Radiation,Skull vasculature,Ultrasound microscopy},\n volume = {133},\n id = {824603ce-60e6-334f-8cce-945bb339129a},\n created = {2020-07-11T11:23:47.526Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:26.755Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Angiogenesis is critical in bone development and growth. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and play an important role in bone repair. Yet, the intricate functional morphology of skull microvasculature remains poorly understood as it is difficult to visualize using existing intravital microscopy techniques. Here we introduced an intravital, fully-transcranial imaging approach based on hybrid optoacoustic and ultrasound bio-microscopy for large-scale observations and quantitative analysis of the vascular morphology, angiogenesis, vessel remodeling, and subsurface roughness in murine skulls. Our approach revealed radiation-inhibited angiogenesis in the skull bone. We also observed previously undocumented sinusoidal vascular networks spanning the entire skullcap, thus opening new vistas for studying the complex interactions between calvarial, pial, and cortical vascular systems.},\n bibtype = {article},\n author = {Estrada, Héctor and Rebling, Johannes and Sievert, Wolfgang and Hladik, Daniela and Hofmann, Urs and Gottschalk, Sven and Tapio, Soile and Multhoff, Gabriele and Razansky, Daniel},\n doi = {10.1016/j.bone.2020.115251},\n journal = {Bone},\n number = {October 2019}\n}\n\n Angiogenesis is critical in bone development and growth. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and play an important role in bone repair. Yet, the intricate functional morphology of skull microvasculature remains poorly understood as it is difficult to visualize using existing intravital microscopy techniques. Here we introduced an intravital, fully-transcranial imaging approach based on hybrid optoacoustic and ultrasound bio-microscopy for large-scale observations and quantitative analysis of the vascular morphology, angiogenesis, vessel remodeling, and subsurface roughness in murine skulls. Our approach revealed radiation-inhibited angiogenesis in the skull bone. We also observed previously undocumented sinusoidal vascular networks spanning the entire skullcap, thus opening new vistas for studying the complex interactions between calvarial, pial, and cortical vascular systems.\n
\n\n\n\n \n\n \n \n \n \n \n Publisher Correction: Rapid volumetric optoacoustic imaging of neural dynamics across the mouse brain (Nature Biomedical Engineering, (2019), 3, 5, (392-401), 10.1038/s41551-019-0372-9).\n \n \n \n\n\n \n Gottschalk, S.; Degtyaruk, O.; Mc Larney, B.; Rebling, J.; Hutter, M.; Deán-Ben, X.; Shoham, S.; and Razansky, D.\n\n\n \n\n\n\n Nature Biomedical Engineering, 4(11). 2020.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Publisher Correction: Rapid volumetric optoacoustic imaging of neural dynamics across the mouse brain (Nature Biomedical Engineering, (2019), 3, 5, (392-401), 10.1038/s41551-019-0372-9)},\n type = {article},\n year = {2020},\n volume = {4},\n id = {8f7daac1-dc1d-3e6f-b1a6-75e0b8daa846},\n created = {2020-11-08T23:59:00.000Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-11-12T13:55:09.539Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n private_publication = {false},\n abstract = {© 2020, The Author(s), under exclusive licence to Springer Nature Limited. An amendment to this paper has been published and can be accessed via a link at the top of the paper.},\n bibtype = {article},\n author = {Gottschalk, S. and Degtyaruk, O. and Mc Larney, B. and Rebling, J. and Hutter, M.A. and Deán-Ben, X.L. and Shoham, S. and Razansky, D.},\n doi = {10.1038/s41551-020-00653-1},\n journal = {Nature Biomedical Engineering},\n number = {11}\n}\n\n © 2020, The Author(s), under exclusive licence to Springer Nature Limited. An amendment to this paper has been published and can be accessed via a link at the top of the paper.\n
\n\n\n\n \n\n \n \n \n \n \n High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy.\n \n \n \n\n\n \n Chen, Z.; Mc Larney, B.; Rebling, J.; Deán-Ben, X.; Zhou, Q.; Gottschalk, S.; and Razansky, D.\n\n\n \n\n\n\n Laser and Photonics Reviews, 14(2). 2020.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n\n\n\n\n\n \n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@article{\n title = {High-Speed Large-Field Multifocal Illumination Fluorescence Microscopy},\n type = {article},\n year = {2020},\n keywords = {diffraction gratings,fast scanning microscopy,fluorescence imaging,multifocal illumination},\n volume = {14},\n id = {f8144dcd-8c1f-3d3f-b089-be96b312f61e},\n created = {2019-12-30T23:59:00.000Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2021-03-02T07:44:13.323Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n private_publication = {true},\n abstract = {© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Scanning optical microscopy techniques are commonly restricted to a sub-millimeter field-of-view (FOV) or otherwise employ slow mechanical translation, limiting their applicability for imaging fast biological dynamics occurring over large areas. A rapid scanning large-field multifocal illumination (LMI) fluorescence microscopy technique is devised based on a beam-splitting grating and an acousto-optic deflector synchronized with a high-speed camera to attain real-time fluorescence microscopy over a centimeter-scale FOV. Owing to its large depth of focus, the approach allows noninvasive visualization of perfusion across the entire mouse cerebral cortex, not achievable with conventional wide-field fluorescence microscopy methods. The new concept can readily be incorporated into conventional wide-field microscopes to mitigate image blur due to tissue scattering and attain optimal trade-off between spatial resolution and FOV. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus it is anticipated to find broad applicability in functional neuroimaging, in vivo cell tracking, and other applications looking at large-scale fluorescent-based biodynamics.},\n bibtype = {article},\n author = {Chen, Z. and Mc Larney, B. and Rebling, J. and Deán-Ben, X.L. and Zhou, Q. and Gottschalk, S. and Razansky, D.},\n doi = {10.1002/lpor.201900070},\n journal = {Laser and Photonics Reviews},\n number = {2}\n}\n\n © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Scanning optical microscopy techniques are commonly restricted to a sub-millimeter field-of-view (FOV) or otherwise employ slow mechanical translation, limiting their applicability for imaging fast biological dynamics occurring over large areas. A rapid scanning large-field multifocal illumination (LMI) fluorescence microscopy technique is devised based on a beam-splitting grating and an acousto-optic deflector synchronized with a high-speed camera to attain real-time fluorescence microscopy over a centimeter-scale FOV. Owing to its large depth of focus, the approach allows noninvasive visualization of perfusion across the entire mouse cerebral cortex, not achievable with conventional wide-field fluorescence microscopy methods. The new concept can readily be incorporated into conventional wide-field microscopes to mitigate image blur due to tissue scattering and attain optimal trade-off between spatial resolution and FOV. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus it is anticipated to find broad applicability in functional neuroimaging, in vivo cell tracking, and other applications looking at large-scale fluorescent-based biodynamics.\n
\n\n\n\n
\n\n \n \n 2019\n \n \n (5)\n \n \n
\n \n \n \n
\n \n \n\n \n \n \n \n \n Uniform light delivery in volumetric optoacoustic tomography.\n \n \n \n\n\n \n Mc Larney, B.; Rebling, J.; Chen, Z.; Deán-Ben, X., L.; Gottschalk, S.; and Razansky, D.\n\n\n \n\n\n\n Journal of Biophotonics, 12(6): e201800387. 2019.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n\n\n \n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n
\n\n\n@article{\n title = {Uniform light delivery in volumetric optoacoustic tomography},\n type = {article},\n year = {2019},\n keywords = {3d-printing,fibers,illumination,imaging,optoacoustics,organs},\n pages = {e201800387},\n volume = {12},\n id = {a8a6e4c1-6efc-37f3-9174-462939c952dd},\n created = {2020-07-11T11:23:47.020Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.020Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non-uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimized illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom-made optical fiber illumination. The custom-designed sample chamber ensures convenient access to the imaged object along with accurate positioning of the sample and a matrix array ultrasound transducer used for collection of the volumetric image data. Ray tracing is employed to optimize the positioning of the individual fibers in the chamber. Homogeneity of the generated light excitation field was confirmed in tissue-mimicking agar spheres. Applicability of the system to image entire mouse organs ex vivo has been showcased. The new approach showed a clear advantage over conventional, single-sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality.},\n bibtype = {article},\n author = {Mc Larney, Benedict and Rebling, Johannes and Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel},\n doi = {10.1002/jbio.201800387},\n journal = {Journal of Biophotonics},\n number = {6}\n}\n\n Accurate image reconstruction in volumetric optoacoustic tomography implies the efficient generation and collection of ultrasound signals around the imaged object. Non-uniform delivery of the excitation light is a common problem in optoacoustic imaging often leading to a diminished field of view, limited dynamic range and penetration, as well as impaired quantification abilities. Presented here is an optimized illumination concept for volumetric tomography that utilizes additive manufacturing via 3D printing in combination with custom-made optical fiber illumination. The custom-designed sample chamber ensures convenient access to the imaged object along with accurate positioning of the sample and a matrix array ultrasound transducer used for collection of the volumetric image data. Ray tracing is employed to optimize the positioning of the individual fibers in the chamber. Homogeneity of the generated light excitation field was confirmed in tissue-mimicking agar spheres. Applicability of the system to image entire mouse organs ex vivo has been showcased. The new approach showed a clear advantage over conventional, single-sided illumination strategies by eliminating the need to correct for illumination variances and resulting in enhancement of the effective field of view, greater penetration depth and significant improvements in the overall image quality.\n
\n\n\n\n \n\n \n \n \n \n \n \n Multifocal structured illumination optoacoustic microscopy.\n \n \n \n \n\n\n \n Chen, Z.; Deán-Ben, X., L.; Özbek, A.; Rebling, J.; and Razansky, D.\n\n\n \n\n\n\n Photons Plus Ultrasound: Imaging and Sensing 2019, 1087816(February): 40. 2019.\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n\n
\n\n\n\n@article{\n title = {Multifocal structured illumination optoacoustic microscopy},\n type = {article},\n year = {2019},\n pages = {40},\n volume = {1087816},\n id = {354de2b2-bfd4-3d3d-8a3e-bedcb4e1b191},\n created = {2020-07-11T11:23:47.204Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-18T08:43:23.845Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n bibtype = {article},\n author = {Chen, Zhenyue and Deán-Ben, Xosé Luis and Özbek, Ali and Rebling, Johannes and Razansky, Daniel},\n doi = {10.1117/12.2508834},\n journal = {Photons Plus Ultrasound: Imaging and Sensing 2019},\n number = {February}\n}\n\n \n\n \n \n \n \n \n \n Rapid volumetric optoacoustic imaging of neural dynamics across the mouse brain.\n \n \n \n \n\n\n \n Gottschalk, S.; Degtyaruk, O.; Mc Larney, B.; Rebling, J.; Hutter, M., A.; Deán-Ben, X., L.; Shoham, S.; and Razansky, D.\n\n\n \n\n\n\n Nature Biomedical Engineering, 3(5): 392-401. 3 2019.\n \n\n\n\n
\n\n\n\n \n \n![\"Rapid](\"//bibbase.org/img/filetypes/link.svg\"\n\t "\\"Website\\"\n\t")
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\n\n\n@article{\n title = {Rapid volumetric optoacoustic imaging of neural dynamics across the mouse brain},\n type = {article},\n year = {2019},\n keywords = {Imaging,Imaging and sensing,Neuroscience,Sensors and probes},\n pages = {392-401},\n volume = {3},\n websites = {http://www.nature.com/articles/s41551-019-0372-9,http://dx.doi.org/10.1038/s41551-019-0372-9},\n month = {3},\n publisher = {Springer US},\n day = {25},\n id = {3344c174-5e52-3572-83ba-f6673b41080e},\n created = {2020-07-11T11:23:47.395Z},\n accessed = {2019-04-02},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.395Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Efforts to scale neuroimaging towards the direct visualization of mammalian brain-wide neuronal activity have faced major challenges. Although high-resolution optical imaging of the whole brain in small animals has been achieved ex vivo, the real-time and direct monitoring of large-scale neuronal activity remains difficult, owing to the performance gap between localized, largely invasive, optical microscopy of rapid, cellular-resolved neuronal activity and whole-brain macroscopy of slow haemodynamics and metabolism. Here, we demonstrate both ex vivo and non-invasive in vivo functional optoacoustic (OA) neuroimaging of mice expressing the genetically encoded calcium indicator GCaMP6f. The approach offers rapid, high-resolution three-dimensional snapshots of whole-brain neuronal activity maps using single OA excitations, and of stimulus-evoked slow haemodynamics and fast calcium activity in the presence of strong haemoglobin background absorption. By providing direct neuroimaging at depths and spatiotemporal resolutions superior to optical fluorescence imaging, functional OA neuroimaging bridges the gap between functional microscopy and whole-brain macroscopy.},\n bibtype = {article},\n author = {Gottschalk, Sven and Degtyaruk, Oleksiy and Mc Larney, Benedict and Rebling, Johannes and Hutter, Magdalena Anastasia and Deán-Ben, Xosé Luís and Shoham, Shy and Razansky, Daniel},\n doi = {10.1038/s41551-019-0372-9},\n journal = {Nature Biomedical Engineering},\n number = {5}\n}\n\n Efforts to scale neuroimaging towards the direct visualization of mammalian brain-wide neuronal activity have faced major challenges. Although high-resolution optical imaging of the whole brain in small animals has been achieved ex vivo, the real-time and direct monitoring of large-scale neuronal activity remains difficult, owing to the performance gap between localized, largely invasive, optical microscopy of rapid, cellular-resolved neuronal activity and whole-brain macroscopy of slow haemodynamics and metabolism. Here, we demonstrate both ex vivo and non-invasive in vivo functional optoacoustic (OA) neuroimaging of mice expressing the genetically encoded calcium indicator GCaMP6f. The approach offers rapid, high-resolution three-dimensional snapshots of whole-brain neuronal activity maps using single OA excitations, and of stimulus-evoked slow haemodynamics and fast calcium activity in the presence of strong haemoglobin background absorption. By providing direct neuroimaging at depths and spatiotemporal resolutions superior to optical fluorescence imaging, functional OA neuroimaging bridges the gap between functional microscopy and whole-brain macroscopy.\n
\n\n\n\n \n\n \n \n \n \n \n Isolated murine brain model for large-scale optoacoustic calcium imaging.\n \n \n \n\n\n \n Gottschalk, S.; Degtyaruk, O.; McLarney, B.; Rebling, J.; Deán-Ben, X.; Shoham, S.; and Razansky, D.\n\n\n \n\n\n\n Frontiers in Neuroscience, 13(APR). 2019.\n \n\n\n\n
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\n\n\n@article{\n title = {Isolated murine brain model for large-scale optoacoustic calcium imaging},\n type = {article},\n year = {2019},\n keywords = {Calcium dynamics,Functional neuroimaging,GCaMP6f,Isolated brain,Optoacoustic neuroimaging},\n volume = {13},\n id = {1b2767a4-48bd-3eaf-9a3c-9957701bd970},\n created = {2019-06-30T23:59:00.000Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-12-26T18:32:33.880Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n private_publication = {false},\n abstract = {Copyright © 2019 Gottschalk, Degtyaruk, Mc Larney, Rebling, Deán-Ben, Shoham and Razansky. Real-time visualization of large-scale neural dynamics in whole mammalian brains is hindered with existing neuroimaging methods having limited capacity when it comes to imaging large tissue volumes at high speeds. Optoacoustic imaging has been shown to be capable of real-time three-dimensional imaging of multiple cerebral hemodynamic parameters in rodents. However, optoacoustic imaging of calcium activity deep within the mammalian brain is hampered by strong blood absorption in the visible light spectrum as well as a lack of activity labels excitable in the near-infrared window. We have developed and validated an isolated whole mouse brain preparation labeled with genetically encoded calcium indicator GCaMP6f, which can closely resemble in vivo conditions. An optoacoustic imaging system coupled to a superfusion system was further designed and used for rapid volumetric monitoring of stimulus-evoked calcium dynamics in the brain. These new imaging setup and isolated preparation's protocols and characteristics are described here in detail. Our new technique captures calcium fluxes as true three-dimensional information across the entire brain with temporal resolution of 10 ms and spatial resolution of 150 μm, thus enabling large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with any existing neuroimaging techniques.},\n bibtype = {article},\n author = {Gottschalk, S. and Degtyaruk, O. and McLarney, B. and Rebling, J. and Deán-Ben, X.L. and Shoham, S. and Razansky, D.},\n doi = {10.3389/fnins.2019.00290},\n journal = {Frontiers in Neuroscience},\n number = {APR}\n}\n\n Copyright © 2019 Gottschalk, Degtyaruk, Mc Larney, Rebling, Deán-Ben, Shoham and Razansky. Real-time visualization of large-scale neural dynamics in whole mammalian brains is hindered with existing neuroimaging methods having limited capacity when it comes to imaging large tissue volumes at high speeds. Optoacoustic imaging has been shown to be capable of real-time three-dimensional imaging of multiple cerebral hemodynamic parameters in rodents. However, optoacoustic imaging of calcium activity deep within the mammalian brain is hampered by strong blood absorption in the visible light spectrum as well as a lack of activity labels excitable in the near-infrared window. We have developed and validated an isolated whole mouse brain preparation labeled with genetically encoded calcium indicator GCaMP6f, which can closely resemble in vivo conditions. An optoacoustic imaging system coupled to a superfusion system was further designed and used for rapid volumetric monitoring of stimulus-evoked calcium dynamics in the brain. These new imaging setup and isolated preparation's protocols and characteristics are described here in detail. Our new technique captures calcium fluxes as true three-dimensional information across the entire brain with temporal resolution of 10 ms and spatial resolution of 150 μm, thus enabling large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with any existing neuroimaging techniques.\n
\n\n\n\n \n\n \n \n \n \n \n High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators.\n \n \n \n\n\n \n Hofmann, U.; Fabritius, A.; Rebling, J.; Estrada, H.; Deán-Ben, X.; Griesbeck, O.; and Razansky, D.\n\n\n \n\n\n\n iScience, 22. 2019.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@article{\n title = {High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators},\n type = {article},\n year = {2019},\n keywords = {Analytical Chemistry,Bioengineering,Biomaterials},\n volume = {22},\n id = {75cb0cee-c3d1-34a5-bbc5-bc6a03c7fb7f},\n created = {2019-12-12T23:59:00.000Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2021-02-26T07:46:52.283Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n private_publication = {true},\n abstract = {© 2019 The Authors Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical microscopic techniques. However, currently available GECIs optimized for fluorescence (FL) imaging lack sufficient contrast for OA imaging and respond at wavelengths having limited penetration into the mammalian brain. Here we present an imaging platform capable of rapid assessment and cross-validation between OA and FL responses of sensor proteins expressed in Escherichia coli colonies. The screening system features optimized pulsed light excitation combined with ultrasensitive ultrasound detection to mitigate photobleaching while further allowing the dynamic characterization of calcium ion responses with millisecond precision. Targeted probing of up to six individual colonies per second in both calcium-loaded and calcium-unloaded states was possible with the system. The new platform greatly facilitates optimization of absorption-based labels, thus setting the stage for directed evolution of OA GECIs.},\n bibtype = {article},\n author = {Hofmann, U.A.T. and Fabritius, A. and Rebling, J. and Estrada, H. and Deán-Ben, X.L. and Griesbeck, O. and Razansky, D.},\n doi = {10.1016/j.isci.2019.11.034},\n journal = {iScience}\n}\n\n © 2019 The Authors Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical microscopic techniques. However, currently available GECIs optimized for fluorescence (FL) imaging lack sufficient contrast for OA imaging and respond at wavelengths having limited penetration into the mammalian brain. Here we present an imaging platform capable of rapid assessment and cross-validation between OA and FL responses of sensor proteins expressed in Escherichia coli colonies. The screening system features optimized pulsed light excitation combined with ultrasensitive ultrasound detection to mitigate photobleaching while further allowing the dynamic characterization of calcium ion responses with millisecond precision. Targeted probing of up to six individual colonies per second in both calcium-loaded and calcium-unloaded states was possible with the system. The new platform greatly facilitates optimization of absorption-based labels, thus setting the stage for directed evolution of OA GECIs.\n
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\n \n \n\n \n \n \n \n \n Looking at the Skull in a New Light: Rayleigh-Lamb Waves in Cranial Bone.\n \n \n \n\n\n \n Estrada, H.; Gottschalk, S.; Reiss, M.; Neuschmelting, V.; Rebling, J.; Goldbrunner, R.; and Razansky, D.\n\n\n \n\n\n\n In IEEE International Ultrasonics Symposium, IUS, volume 2018-Octob, 2018. \n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Looking at the Skull in a New Light: Rayleigh-Lamb Waves in Cranial Bone},\n type = {inproceedings},\n year = {2018},\n keywords = {Lamb Waves,Laser ultrasonics,Near Field,Plate waves,Rayleigh Waves,Skull Bone,Surface Acoustic Waves},\n volume = {2018-Octob},\n id = {c11a3e31-3d79-3f3f-b4cb-a5084c3f2803},\n created = {2019-03-13T14:00:29.648Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.867Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Current knowledge on the ultrasound wave propagation in the cranial bone is restricted to far-field observations. In order to extend our understanding on how ultrasound waves propagate in the skull, we use short laser pulses to excite ultrasound waves in water-immersed ex vivo mouse and human skulls and explored their near-field. The laser pulses (10 ns duration) of 532 nm are absorbed by a small layer of black burnish deposited on the skull's inner surface and generate ultrasound waves due to the thermoelastic effect. The acoustic near-field is mapped using a needle hydrophone close to the skull surface, following a three-dimensional scanning path derived from a previous pulse-echo scan of the skull with a spherically focused ultrasound transducer. The results for mouse and human skulls show different wave propagation regimes according to their differences in size, thickness, and internal structure. Leaky and non-leaky waves have been observed for both skull samples. Zero order Lamb modes were observed in the mouse skull, whereas Rayleigh-Lamb higher order modes can be observed in the human skull sample, presumably propagating in the outer cortical bone layer. Good agreement is found between the experiments and the multilayered flat plate model.},\n bibtype = {inproceedings},\n author = {Estrada, Hector and Gottschalk, Sven and Reiss, Michael and Neuschmelting, Volker and Rebling, Johannes and Goldbrunner, Roland and Razansky, Daniel},\n doi = {10.1109/ULTSYM.2018.8580113},\n booktitle = {IEEE International Ultrasonics Symposium, IUS}\n}\n\n Current knowledge on the ultrasound wave propagation in the cranial bone is restricted to far-field observations. In order to extend our understanding on how ultrasound waves propagate in the skull, we use short laser pulses to excite ultrasound waves in water-immersed ex vivo mouse and human skulls and explored their near-field. The laser pulses (10 ns duration) of 532 nm are absorbed by a small layer of black burnish deposited on the skull's inner surface and generate ultrasound waves due to the thermoelastic effect. The acoustic near-field is mapped using a needle hydrophone close to the skull surface, following a three-dimensional scanning path derived from a previous pulse-echo scan of the skull with a spherically focused ultrasound transducer. The results for mouse and human skulls show different wave propagation regimes according to their differences in size, thickness, and internal structure. Leaky and non-leaky waves have been observed for both skull samples. Zero order Lamb modes were observed in the mouse skull, whereas Rayleigh-Lamb higher order modes can be observed in the human skull sample, presumably propagating in the outer cortical bone layer. Good agreement is found between the experiments and the multilayered flat plate model.\n
\n\n\n\n \n\n \n \n \n \n \n In-vivo assessment of radiation-induced cerebrovascular damage in mice by hybrid optoacoustic-ultrasound bio-microscopy (Conference Presentation).\n \n \n \n\n\n \n Rebling, J.; Estrada, H.; Sievert, W.; Hladik, D.; Tapio, S.; and Razansky, D.\n\n\n \n\n\n\n In Photons Plus Ultrasound: Imaging and Sensing 2018, volume 10494, pages 17, 2018. International Society for Optics and Photonics\n \n\n\n\n
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\n\n\n@inproceedings{\n title = {In-vivo assessment of radiation-induced cerebrovascular damage in mice by hybrid optoacoustic-ultrasound bio-microscopy (Conference Presentation)},\n type = {inproceedings},\n year = {2018},\n pages = {17},\n volume = {10494},\n publisher = {International Society for Optics and Photonics},\n id = {b67db354-6e3c-3e28-a4ca-f5d3f6441159},\n created = {2020-07-11T11:23:46.624Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:46.624Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {Radiation-induced brain damage could arise as a side effect in radiotherapy of brain tumors. We present evidence of radiation-induced damage to the skull and brain vasculature of mice as revealed by transcranial optoacoustic and ultrasound bio-microscopy in-vivo. The three-dimensional nature of the acquired optoacoustic images combined with a clear anatomical reference of the pulse-echo ultrasound data allowed clear differentiation of the skull vasculature from the superficial brain vasculature. The irradiation was selectively applied to one brain hemisphere and the effects of the ionizing radiation were evident without introduction of extrinsic labeling, owing to the strong haemoglobin contrast of optoacoustics.},\n bibtype = {inproceedings},\n author = {Rebling, Johannes and Estrada, Héctor and Sievert, Wolfgang and Hladik, Daniela and Tapio, Soile and Razansky, Daniel},\n doi = {10.1117/12.2290493},\n booktitle = {Photons Plus Ultrasound: Imaging and Sensing 2018}\n}\n\n Radiation-induced brain damage could arise as a side effect in radiotherapy of brain tumors. We present evidence of radiation-induced damage to the skull and brain vasculature of mice as revealed by transcranial optoacoustic and ultrasound bio-microscopy in-vivo. The three-dimensional nature of the acquired optoacoustic images combined with a clear anatomical reference of the pulse-echo ultrasound data allowed clear differentiation of the skull vasculature from the superficial brain vasculature. The irradiation was selectively applied to one brain hemisphere and the effects of the ionizing radiation were evident without introduction of extrinsic labeling, owing to the strong haemoglobin contrast of optoacoustics.\n
\n\n\n\n \n\n \n \n \n \n \n \n Virtual craniotomy for high-resolution optoacoustic brain microscopy.\n \n \n \n \n\n\n \n Estrada, H.; Huang, X.; Rebling, J.; Zwack, M.; Gottschalk, S.; and Razansky, D.\n\n\n \n\n\n\n Scientific Reports, 8(1): 1459. 12 2018.\n \n\n\n\n
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\n\n\n@article{\n title = {Virtual craniotomy for high-resolution optoacoustic brain microscopy},\n type = {article},\n year = {2018},\n pages = {1459},\n volume = {8},\n websites = {http://www.nature.com/articles/s41598-017-18857-y},\n month = {12},\n day = {23},\n id = {980c52b5-94cb-3c18-b2f1-4889b1aaf0ce},\n created = {2020-07-11T11:23:46.624Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:31.051Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {Ultrasound-mediated transcranial images of the brain often suffer from acoustic distortions produced by the skull bone. In high-resolution optoacoustic microscopy, the skull-induced acoustic aberrations are known to impair image resolution and contrast, further skewing the location and intensity of the different absorbing structures. We present a virtual craniotomy deconvolution algorithm based on an ultrasound wave propagation model that corrects for the skull-induced distortions in optically-resolved optoacoustic transcranial microscopy data. The method takes advantage of the geometrical and spectral information of a pulse-echo ultrasound image of the skull simultaneously acquired by our multimodal imaging system. Transcranial mouse brain imaging experiments confirmed the ability to accurately account for the signal amplitude decay, temporal delay and pulse broadening introduced by the rodent's skull. Our study is the first to demonstrate skull-corrected transcranial optoacoustic imaging in vivo.},\n bibtype = {article},\n author = {Estrada, Héctor and Huang, Xiao and Rebling, Johannes and Zwack, Michael and Gottschalk, Sven and Razansky, Daniel},\n doi = {10.1038/s41598-017-18857-y},\n journal = {Scientific Reports},\n number = {1}\n}\n\n Ultrasound-mediated transcranial images of the brain often suffer from acoustic distortions produced by the skull bone. In high-resolution optoacoustic microscopy, the skull-induced acoustic aberrations are known to impair image resolution and contrast, further skewing the location and intensity of the different absorbing structures. We present a virtual craniotomy deconvolution algorithm based on an ultrasound wave propagation model that corrects for the skull-induced distortions in optically-resolved optoacoustic transcranial microscopy data. The method takes advantage of the geometrical and spectral information of a pulse-echo ultrasound image of the skull simultaneously acquired by our multimodal imaging system. Transcranial mouse brain imaging experiments confirmed the ability to accurately account for the signal amplitude decay, temporal delay and pulse broadening introduced by the rodent's skull. Our study is the first to demonstrate skull-corrected transcranial optoacoustic imaging in vivo.\n
\n\n\n\n \n\n \n \n \n \n \n \n Dual-wavelength hybrid optoacoustic-ultrasound biomicroscopy for functional imaging of large-scale cerebral vascular networks.\n \n \n \n \n\n\n \n Rebling, J.; Estrada, H.; Gottschalk, S.; Sela, G.; Zwack, M.; Wissmeyer, G.; Ntziachristos, V.; and Razansky, D.\n\n\n \n\n\n\n Journal of Biophotonics, 11(9): e201800057. 2 2018.\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n \n Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Dual-wavelength hybrid optoacoustic-ultrasound biomicroscopy for functional imaging of large-scale cerebral vascular networks},\n type = {article},\n year = {2018},\n keywords = {blood vessels,brain,cerebral,microscopy,photoacoustics,ultrasound},\n pages = {e201800057},\n volume = {11},\n websites = {http://doi.wiley.com/10.1002/jbio.201800057},\n month = {2},\n id = {230bb056-7f8d-3036-82b4-b3e6e9863164},\n created = {2020-07-11T11:23:46.635Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:28.559Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Article},\n private_publication = {false},\n abstract = {A critical link exists between pathological changes of cerebral vasculature and diseases affecting brain function. Microscopic techniques have played an indispensable role in the study of neurovascular anatomy and functions. Yet, investigations are often hindered by suboptimal trade-offs between the spatiotemporal resolution, field-of-view (FOV) and type of contrast offered by the existing optical microscopy techniques. We present a hybrid dual-wavelength optoacoustic (OA) biomicroscope capable of rapid transcranial visualization of large-scale cerebral vascular networks. The system offers 3-dimensional views of the morphology and oxygenation status of the cerebral vasculature with single capillary resolution and a FOV exceeding 6 × 8 mm2, thus covering the entire cortical vasculature in mice. The large-scale OA imaging capacity is complemented by simultaneously acquired pulse-echo ultrasound (US) biomicroscopy scans of the mouse skull. The new approach holds great potential to provide better insights into cerebrovascular function and facilitate efficient studies into neurological and vascular abnormalities of the brain.},\n bibtype = {article},\n author = {Rebling, Johannes and Estrada, Héctor and Gottschalk, Sven and Sela, Gali and Zwack, Michael and Wissmeyer, Georg and Ntziachristos, Vasilis and Razansky, Daniel},\n doi = {10.1002/jbio.201800057},\n journal = {Journal of Biophotonics},\n number = {9}\n}\n\n A critical link exists between pathological changes of cerebral vasculature and diseases affecting brain function. Microscopic techniques have played an indispensable role in the study of neurovascular anatomy and functions. Yet, investigations are often hindered by suboptimal trade-offs between the spatiotemporal resolution, field-of-view (FOV) and type of contrast offered by the existing optical microscopy techniques. We present a hybrid dual-wavelength optoacoustic (OA) biomicroscope capable of rapid transcranial visualization of large-scale cerebral vascular networks. The system offers 3-dimensional views of the morphology and oxygenation status of the cerebral vasculature with single capillary resolution and a FOV exceeding 6 × 8 mm2, thus covering the entire cortical vasculature in mice. The large-scale OA imaging capacity is complemented by simultaneously acquired pulse-echo ultrasound (US) biomicroscopy scans of the mouse skull. The new approach holds great potential to provide better insights into cerebrovascular function and facilitate efficient studies into neurological and vascular abnormalities of the brain.\n
\n\n\n\n \n\n \n \n \n \n \n Multifocal structured illumination fluorescence microscopy with large field-of-view and high spatio-temporal resolution.\n \n \n \n\n\n \n Chen, Z.; McLarney, B.; Rebling, J.; Deán-Ben, X., L.; Razansky, D.; and Gottschalk, S.\n\n\n \n\n\n\n In Proceedings of SPIE - The International Society for Optical Engineering, volume 10816, pages 5, 2018. International Society for Optics and Photonics\n \n\n\n\n
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\n\n\n \n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Multifocal structured illumination fluorescence microscopy with large field-of-view and high spatio-temporal resolution},\n type = {inproceedings},\n year = {2018},\n keywords = {fluorescence imaging,high speed,large field of view,multifocal structured illumination},\n pages = {5},\n volume = {10816},\n publisher = {International Society for Optics and Photonics},\n id = {f8c97086-ddda-34b0-ab1c-7225963aa6b1},\n created = {2020-07-11T11:23:47.327Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.327Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© 2018 Copyright SPIE. Fluorescence imaging is widely employed in biological discovery due to its excellent molecular sensitivity and contrast. However, due to light scattering wide-field fluorescence images are blurred resulting in very low spatial resolution and low image contrast. The existing scanning optical microscopy techniques are commonly restricted to sub-millimeter field-of-view or otherwise slow imaging speeds, limiting their applicability for imaging of fast biological dynamics occurring on larger spatial scales. Herein, we developed a rapid scanning wide-field multifocal structured illumination microscopy method based on a beam-splitting grating and an acousto-optic deflector synchronized with a high speed camera. The multi-beam pattern is focused by a condensing lens and a macroscopic objective to generate multifocal structured illumination profile on the imaged sample that is rapidly scanned at kHz rates. Experimental results show that the proposed method can achieve real-time fluorescence microscopy over a centimeter-scale field of view. Owing to the low numerical aperture of the diffracted beams, the illumination has a large depth of focus and hence is generally not affected by the sample's curvature, which allowed here imaging of perfusion in the entire mouse cerebral cortex noninvasively. The new approach can be readily incorporated into traditional wide-field microscopes to attain optimal tradeoff between spatial resolution and field of view. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus anticipated to find broad applicability in a variety of applications looking at large-scale fluorescent-based biodynamics.},\n bibtype = {inproceedings},\n author = {Chen, Zhenyue and McLarney, Ben and Rebling, Johannes and Deán-Ben, Xosé Luis and Razansky, Daniel and Gottschalk, Sven},\n doi = {10.1117/12.2500907},\n booktitle = {Proceedings of SPIE - The International Society for Optical Engineering}\n}\n\n © 2018 Copyright SPIE. Fluorescence imaging is widely employed in biological discovery due to its excellent molecular sensitivity and contrast. However, due to light scattering wide-field fluorescence images are blurred resulting in very low spatial resolution and low image contrast. The existing scanning optical microscopy techniques are commonly restricted to sub-millimeter field-of-view or otherwise slow imaging speeds, limiting their applicability for imaging of fast biological dynamics occurring on larger spatial scales. Herein, we developed a rapid scanning wide-field multifocal structured illumination microscopy method based on a beam-splitting grating and an acousto-optic deflector synchronized with a high speed camera. The multi-beam pattern is focused by a condensing lens and a macroscopic objective to generate multifocal structured illumination profile on the imaged sample that is rapidly scanned at kHz rates. Experimental results show that the proposed method can achieve real-time fluorescence microscopy over a centimeter-scale field of view. Owing to the low numerical aperture of the diffracted beams, the illumination has a large depth of focus and hence is generally not affected by the sample's curvature, which allowed here imaging of perfusion in the entire mouse cerebral cortex noninvasively. The new approach can be readily incorporated into traditional wide-field microscopes to attain optimal tradeoff between spatial resolution and field of view. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus anticipated to find broad applicability in a variety of applications looking at large-scale fluorescent-based biodynamics.\n
\n\n\n\n \n\n \n \n \n \n \n A new catheter design for combined radiofrequency ablation and optoacoustic treatment monitoring using copper-coated light-guides.\n \n \n \n\n\n \n Rebling, J.; Oyaga Landa, F., J.; Deán-Ben, X., L.; and Razansky, D.\n\n\n \n\n\n\n In Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII, volume 10488, pages 4, 2018. International Society for Optics and Photonics\n \n\n\n\n
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\n\n\n@inproceedings{\n title = {A new catheter design for combined radiofrequency ablation and optoacoustic treatment monitoring using copper-coated light-guides},\n type = {inproceedings},\n year = {2018},\n keywords = {ablation catheter,cardiac,photoacoustic,tomography},\n pages = {4},\n volume = {10488},\n publisher = {International Society for Optics and Photonics},\n id = {0d3739cd-05f7-341d-945f-49cd97f959b0},\n created = {2020-07-11T11:23:47.331Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.331Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {Copyright © 2018 SPIE. Electrosurgery, i.e. the application of radiofrequency current for tissue ablation, is a frequently used treatment for many cardiac arrhythmias. Electrophysiological and anatomic mapping, as well as careful radiofrequency power control typically guide the radiofrequency ablation procedure. Despite its widespread application, accurate monitoring of the lesion formation with sufficient spatio-temporal resolution remains challenging with the existing imaging techniques. We present a novel integrated catheter for simultaneous radiofrequency ablation and optoacoustic monitoring of the lesion formation in real time and 3D. The design combines the delivery of both electric current and optoacoustic excitation beam in a single catheter consisting of copper-coated multimode light-guides and its manufacturing is described in detail. The electrical current causes coagulation and desiccation while the excitation light is locally absorbed, generating OA responses from the entire treated volume. The combined ablation-monitoring capabilities were verified using ex-vivo bovine tissue. The formed ablation lesions showed a homogenous coagulation while the ablation was monitored in realtime with a volumetric frame rate of 10 Hz over 150 seconds.},\n bibtype = {inproceedings},\n author = {Rebling, Johannes and Oyaga Landa, Francisco J. and Deán-Ben, Xosé L. and Razansky, Daniel},\n doi = {10.1117/12.2287390},\n booktitle = {Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII}\n}\n\n Copyright © 2018 SPIE. Electrosurgery, i.e. the application of radiofrequency current for tissue ablation, is a frequently used treatment for many cardiac arrhythmias. Electrophysiological and anatomic mapping, as well as careful radiofrequency power control typically guide the radiofrequency ablation procedure. Despite its widespread application, accurate monitoring of the lesion formation with sufficient spatio-temporal resolution remains challenging with the existing imaging techniques. We present a novel integrated catheter for simultaneous radiofrequency ablation and optoacoustic monitoring of the lesion formation in real time and 3D. The design combines the delivery of both electric current and optoacoustic excitation beam in a single catheter consisting of copper-coated multimode light-guides and its manufacturing is described in detail. The electrical current causes coagulation and desiccation while the excitation light is locally absorbed, generating OA responses from the entire treated volume. The combined ablation-monitoring capabilities were verified using ex-vivo bovine tissue. The formed ablation lesions showed a homogenous coagulation while the ablation was monitored in realtime with a volumetric frame rate of 10 Hz over 150 seconds.\n
\n\n\n\n \n\n \n \n \n \n \n \n Intravital optoacoustic ultrasound bio-microscopy reveals radiation-induced skull vasculopathy.\n \n \n \n \n\n\n \n Estrada, H.; Rebling, J.; Sievert, W.; Hladik, D.; Hofmann, U.; Gottschalk, S.; Tapio, S.; Multhoff, G.; and Razansky, D.\n\n\n \n\n\n\n bioRxiv,500017. 2018.\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n \n Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Intravital optoacoustic ultrasound bio-microscopy reveals radiation-induced skull vasculopathy},\n type = {article},\n year = {2018},\n pages = {500017},\n websites = {https://www.biorxiv.org/content/early/2018/12/19/500017.abstract},\n publisher = {Cold Spring Harbor Laboratory},\n id = {ce8854a3-7c04-3ca5-a3cc-b6965df53c07},\n created = {2020-07-11T11:23:47.477Z},\n accessed = {2019-11-12},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:20.627Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Mounting experimental evidence reveals severe long-term alterations of bone vasculature following ionizing irradiation procedures. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and are essential for their development, hematopoiesis, and maintenance of the bone marrow. Microvasculature inside an intact cranial bone is difficult to visualize using existing intravital microscopy techniques, therefore its functional morphology remained poorly understood. Here, a novel transcranial imaging approach is introduced, based on hybrid optoacoustic and ultrasound bio-microscopy, allowing for large-scale observations and quantitative analysis of the subsurface roughness, vascular morphology, angiogenesis, and vessel remodeling in murine skulls. Our approach enables, for the first time, high-throughput physiological studies to understand radiation-induced vasculopathy in the skull bone. We also observe previously undocumented sinusoidal vascular networks spanning the entire skullcap and report on an extensive reduction of calvarian vasculature following radiation treatments.},\n bibtype = {article},\n author = {Estrada, Héctor and Rebling, Johannes and Sievert, Wolfgang and Hladik, Daniela and Hofmann, Urs and Gottschalk, Sven and Tapio, Soile and Multhoff, Gabriele and Razansky, Daniel},\n doi = {10.1101/500017},\n journal = {bioRxiv}\n}\n\n Mounting experimental evidence reveals severe long-term alterations of bone vasculature following ionizing irradiation procedures. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and are essential for their development, hematopoiesis, and maintenance of the bone marrow. Microvasculature inside an intact cranial bone is difficult to visualize using existing intravital microscopy techniques, therefore its functional morphology remained poorly understood. Here, a novel transcranial imaging approach is introduced, based on hybrid optoacoustic and ultrasound bio-microscopy, allowing for large-scale observations and quantitative analysis of the subsurface roughness, vascular morphology, angiogenesis, and vessel remodeling in murine skulls. Our approach enables, for the first time, high-throughput physiological studies to understand radiation-induced vasculopathy in the skull bone. We also observe previously undocumented sinusoidal vascular networks spanning the entire skullcap and report on an extensive reduction of calvarian vasculature following radiation treatments.\n
\n\n\n\n \n\n \n \n \n \n \n \n Integrated catheter for simultaneous radio frequency ablation and optoacoustic monitoring of lesion progression.\n \n \n \n \n\n\n \n Rebling, J.; Oyaga Landa, F., J.; Deán-Ben, X., L.; Douplik, A.; and Razansky, D.\n\n\n \n\n\n\n Optics Letters, 43(8): 1886. 2018.\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Integrated catheter for simultaneous radio frequency ablation and optoacoustic monitoring of lesion progression},\n type = {article},\n year = {2018},\n pages = {1886},\n volume = {43},\n id = {38213764-8835-34d0-9e0c-c0cbca270caf},\n created = {2020-07-11T11:23:47.493Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:17.106Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {© 2018 Optical Society of America. Radio frequency (RF) catheter ablation is commonly used to eliminate dysfunctional cardiac tissue by heating via an alternating current. Clinical outcomes are highly dependent on careful anatomical guidance, electrophysiological mapping, and careful RF power titration during the procedure. Yet, current treatments rely mainly on the expertise of the surgeon to assess lesion formation, causing large variabilities in the success rate. We present an integrated catheter design suitable for simultaneous RF ablation and real-time optoacoustic monitoring of the forming lesion. The catheter design utilizes copper-coated multimode light guides capable of delivering both ablation current and near-infrared pulsed-laser illumination to the target tissue. The generated optoacoustic responses were used to visualize the ablation lesion formation in an ex-vivo bovine heart specimen in 3D. The presented catheter design enables the monitoring of ablation lesions with high spatiotemporal resolution while the overall therapy-monitoring approach remains compatible with commercially available catheter designs.},\n bibtype = {article},\n author = {Rebling, Johannes and Oyaga Landa, Francisco Javier and Deán-Ben, Xosé Luís and Douplik, Alexandre and Razansky, Daniel},\n doi = {10.1364/ol.43.001886},\n journal = {Optics Letters},\n number = {8}\n}\n\n © 2018 Optical Society of America. Radio frequency (RF) catheter ablation is commonly used to eliminate dysfunctional cardiac tissue by heating via an alternating current. Clinical outcomes are highly dependent on careful anatomical guidance, electrophysiological mapping, and careful RF power titration during the procedure. Yet, current treatments rely mainly on the expertise of the surgeon to assess lesion formation, causing large variabilities in the success rate. We present an integrated catheter design suitable for simultaneous RF ablation and real-time optoacoustic monitoring of the forming lesion. The catheter design utilizes copper-coated multimode light guides capable of delivering both ablation current and near-infrared pulsed-laser illumination to the target tissue. The generated optoacoustic responses were used to visualize the ablation lesion formation in an ex-vivo bovine heart specimen in 3D. The presented catheter design enables the monitoring of ablation lesions with high spatiotemporal resolution while the overall therapy-monitoring approach remains compatible with commercially available catheter designs.\n
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\n \n \n\n \n \n \n \n \n Imaging of post-embryonic stage model organisms at high resolution using multi-orientation optoacoustic mesoscopy.\n \n \n \n\n\n \n Omar, M.; Rebling, J.; Wicker, K.; Schmitt-Manderbach, T.; Schwarz, M.; Gateau, J.; Lopez-Schier, H.; Mappes, T.; and Ntziachristos, V.\n\n\n \n\n\n\n In Photons Plus Ultrasound: Imaging and Sensing 2017, volume 10064, pages 100640Z, 2017. \n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Imaging of post-embryonic stage model organisms at high resolution using multi-orientation optoacoustic mesoscopy},\n type = {inproceedings},\n year = {2017},\n keywords = {Beam-forming,Mesoscopy,Microscopy,Multiview,Optoacoustics},\n pages = {100640Z},\n volume = {10064},\n id = {d149ac8f-a7f5-3758-8400-cc7111281cde},\n created = {2019-03-13T14:00:29.784Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2019-05-29T12:43:02.701Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {© 2017 SPIE. Model organisms such as zebrafish play an important role for developmental biologists and experimental geneticists. Still, as they grow into their post-embryonic stage of development it becomes more and more difficult to image them because of high light scattering inside biological tissue. Optoacoustic mesoscopy based on spherically focused, high frequency, ultrasound detectors offers an alternative, where it relies on the focusing capabilities of the ultrasound detectors in generating the image rather than on the focusing of light. Nonetheless, because of the limited numerical aperture the resolution is not isotropic, and many structures, especially elongated ones, such as blood vessels and other organs, are either invisible, or not clearly identifiable on the final image. Herein, based on high frequency ultrasound detectors at 100 MHz and 50 MHz we introduce multi orientation (view) optoacoustic mesoscopy. We collect a rich amount of signals from multiple directions and combine them using a weighted sum in the Fourier domain and a Wiener deconvolution into a single high resolution three-dimensional image. The new system achieves isotropic resolutions on the order of 10 μm in-plane, 40 μm axially, and SNR enhancement of 15 dB compared to the single orientation case. To showcase the system we imaged a juvenile zebrafish ex vivo, which is too large to image using optical microscopic techniques, the reconstructed images show unprecedented performance in terms of SNR, resolution, and clarity of the observed structures. Using the system we see the inner organs of the zebrafish, the pigmentation, and the vessels with unprecedented clarity.},\n bibtype = {inproceedings},\n author = {Omar, Murad and Rebling, Johannes and Wicker, Kai and Schmitt-Manderbach, Tobias and Schwarz, Mathias and Gateau, Jerome and Lopez-Schier, Hernan and Mappes, Timo and Ntziachristos, Vasilis},\n doi = {10.1117/12.2251454},\n booktitle = {Photons Plus Ultrasound: Imaging and Sensing 2017}\n}\n\n © 2017 SPIE. Model organisms such as zebrafish play an important role for developmental biologists and experimental geneticists. Still, as they grow into their post-embryonic stage of development it becomes more and more difficult to image them because of high light scattering inside biological tissue. Optoacoustic mesoscopy based on spherically focused, high frequency, ultrasound detectors offers an alternative, where it relies on the focusing capabilities of the ultrasound detectors in generating the image rather than on the focusing of light. Nonetheless, because of the limited numerical aperture the resolution is not isotropic, and many structures, especially elongated ones, such as blood vessels and other organs, are either invisible, or not clearly identifiable on the final image. Herein, based on high frequency ultrasound detectors at 100 MHz and 50 MHz we introduce multi orientation (view) optoacoustic mesoscopy. We collect a rich amount of signals from multiple directions and combine them using a weighted sum in the Fourier domain and a Wiener deconvolution into a single high resolution three-dimensional image. The new system achieves isotropic resolutions on the order of 10 μm in-plane, 40 μm axially, and SNR enhancement of 15 dB compared to the single orientation case. To showcase the system we imaged a juvenile zebrafish ex vivo, which is too large to image using optical microscopic techniques, the reconstructed images show unprecedented performance in terms of SNR, resolution, and clarity of the observed structures. Using the system we see the inner organs of the zebrafish, the pigmentation, and the vessels with unprecedented clarity.\n
\n\n\n\n \n\n \n \n \n \n \n \n Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy.\n \n \n \n \n\n\n \n Omar, M.; Rebling, J.; Wicker, K.; Schmitt-Manderbach, T.; Schwarz, M.; Gateau, J.; López-Schier, H.; Mappes, T.; and Ntziachristos, V.\n\n\n \n\n\n\n Light: Science and Applications, 6(1): e16186-e16186. 1 2017.\n \n\n\n\n
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\n\n\n@article{\n title = {Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy},\n type = {article},\n year = {2017},\n keywords = {deconvolution,development,mesoscopy,multiview,optoacoustics,photoacoustics},\n pages = {e16186-e16186},\n volume = {6},\n websites = {http://www.nature.com/articles/lsa2016186},\n month = {1},\n day = {11},\n id = {6642caf7-190d-37a2-ba94-01483dea8fa5},\n created = {2019-03-13T14:00:29.839Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:16:06.756Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.},\n bibtype = {article},\n author = {Omar, Murad and Rebling, Johannes and Wicker, Kai and Schmitt-Manderbach, Tobias and Schwarz, Mathias and Gateau, Jérôme and López-Schier, Hérnan and Mappes, Timo and Ntziachristos, Vasilis},\n doi = {10.1038/lsa.2016.186},\n journal = {Light: Science and Applications},\n number = {1}\n}\n\n Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise, as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However, optoacoustic mesoscopic techniques only offer partial visibility of oriented structures, such as blood vessels, due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9-17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms, with possible applications in the developmental biology of adulthood and aging.\n
\n\n\n\n \n\n \n \n \n \n \n Observation of skull-guided acoustic waves in a water-immersed murine skull using optoacoustic excitation.\n \n \n \n\n\n \n Estrada, H.; Rebling, J.; and Razansky, D.\n\n\n \n\n\n\n In Optical Elastography and Tissue Biomechanics IV, volume 10067, pages 1006710, 2017. International Society for Optics and Photonics\n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Observation of skull-guided acoustic waves in a water-immersed murine skull using optoacoustic excitation},\n type = {inproceedings},\n year = {2017},\n keywords = {Acoustic guided waves,Lamb waves,Laser ultrasonics,Mouse skull,Neuroimaging,Optoacoustic,Photoacoustic},\n pages = {1006710},\n volume = {10067},\n publisher = {International Society for Optics and Photonics},\n id = {2fb477fe-655f-309a-a784-d0e8a665f849},\n created = {2020-07-11T11:23:46.625Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:46.625Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© 2017 SPIE. The skull bone, a curved solid multilayered plate protecting the brain, constitutes a big challenge for the use of ultrasound-mediated techniques in neuroscience. Ultrasound waves incident from water or soft biological tissue are mostly reflected when impinging on the skull. To this end, skull properties have been characterized for both high-intensity focused ultrasound (HIFU) operating in the narrowband far-field regime and optoacoustic imaging applications. Yet, no study has been conducted to characterize the near-field of water immersed skulls. We used the thermoelastic effect with a 532 nm pulsed laser to trigger a wide range of broad-band ultrasound modes in a mouse skull. In order to capture the waves propagating in the near-field, a thin hydrophone was scanned in close proximity to the skull's surface. While Leaky pseudo-Lamb waves and grazing-angle bulk water waves are clearly visible in the spatio-temporal data, we were only able to identify skull-guided acoustic waves after dispersion analysis in the wavenumber-frequency space. The experimental data was found to be in a reasonable agreement with a flat multilayered plate model.},\n bibtype = {inproceedings},\n author = {Estrada, Héctor and Rebling, Johannes and Razansky, Daniel},\n doi = {10.1117/12.2252089},\n booktitle = {Optical Elastography and Tissue Biomechanics IV}\n}\n\n © 2017 SPIE. The skull bone, a curved solid multilayered plate protecting the brain, constitutes a big challenge for the use of ultrasound-mediated techniques in neuroscience. Ultrasound waves incident from water or soft biological tissue are mostly reflected when impinging on the skull. To this end, skull properties have been characterized for both high-intensity focused ultrasound (HIFU) operating in the narrowband far-field regime and optoacoustic imaging applications. Yet, no study has been conducted to characterize the near-field of water immersed skulls. We used the thermoelastic effect with a 532 nm pulsed laser to trigger a wide range of broad-band ultrasound modes in a mouse skull. In order to capture the waves propagating in the near-field, a thin hydrophone was scanned in close proximity to the skull's surface. While Leaky pseudo-Lamb waves and grazing-angle bulk water waves are clearly visible in the spatio-temporal data, we were only able to identify skull-guided acoustic waves after dispersion analysis in the wavenumber-frequency space. The experimental data was found to be in a reasonable agreement with a flat multilayered plate model.\n
\n\n\n\n \n\n \n \n \n \n \n Structural and functional small animal imaging using hybrid-focus optoacoustic biomicroscopy.\n \n \n \n\n\n \n Rebling, J.; Estrada, H.; Gottschalk, S.; and Razansky, D.\n\n\n \n\n\n\n In Optics InfoBase Conference Papers, volume Part F61-E, pages 3, 2017. Optical Society of America\n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Structural and functional small animal imaging using hybrid-focus optoacoustic biomicroscopy},\n type = {inproceedings},\n year = {2017},\n pages = {3},\n volume = {Part F61-E},\n publisher = {Optical Society of America},\n id = {3ae2cfa0-9472-3871-85a0-dcbd7dab060b},\n created = {2020-07-11T11:23:46.631Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:46.631Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {We present an optoacoustic microscope, combining structural and functional opticalresolution optoacoustic and ultrasound pulse-echo imaging. The system was applied to image Zebrafish larvae and complex vascular networks in the murine brain and ear.},\n bibtype = {inproceedings},\n author = {Rebling, Johannes and Estrada, Héctor and Gottschalk, Sven and Razansky, Daniel},\n doi = {10.1117/12.2284656},\n booktitle = {Optics InfoBase Conference Papers}\n}\n\n We present an optoacoustic microscope, combining structural and functional opticalresolution optoacoustic and ultrasound pulse-echo imaging. The system was applied to image Zebrafish larvae and complex vascular networks in the murine brain and ear.\n
\n\n\n\n \n\n \n \n \n \n \n \n Hybrid ultrasound and dual-wavelength optoacoustic biomicroscopy for functional neuroimaging.\n \n \n \n \n\n\n \n Rebling, J.; Estrada, H.; Zwack, M.; Sela, G.; Gottschalk, S.; and Razansky, D.\n\n\n \n\n\n\n In Oraevsky, A., A.; and Wang, L., V., editor(s), Photons Plus Ultrasound: Imaging and Sensing 2017, volume 10064, pages 100644T, 3 2017. International Society for Optics and Photonics\n \n\n\n\n
\n\n\n\n \n \nWebsite\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Hybrid ultrasound and dual-wavelength optoacoustic biomicroscopy for functional neuroimaging},\n type = {inproceedings},\n year = {2017},\n keywords = {Brain imaging,Cerebral vasculature,Multiwavelength,Neuroimaging,Neurovasculature,Photoacoustic},\n pages = {100644T},\n volume = {10064},\n websites = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?doi=10.1117/12.2250635},\n month = {3},\n publisher = {International Society for Optics and Photonics},\n day = {3},\n id = {40c8d52b-0034-3692-b0c3-18e27750a6db},\n created = {2020-07-11T11:23:47.143Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.143Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© 2017 SPIE. Many neurological disorders are linked to abnormal activation or pathological alterations of the vasculature in the affected brain region. Obtaining simultaneous morphological and physiological information of neurovasculature is very challenging due to the acoustic distortions and intense light scattering by the skull and brain. In addition, the size of cerebral vasculature in murine brains spans an extended range from just a few microns up to about a millimeter, all to be recorded in 3D and over an area of several dozens of mm2. Numerous imaging techniques exist that excel at characterizing certain aspects of this complex network but are only capable of providing information on a limited spatiotemporal scale. We present a hybrid ultrasound and dual-wavelength optoacoustic microscope, capable of rapid imaging of murine neurovasculature in-vivo, with high spatial resolution down to 12 μm over a large field of view exceeding 50mm2. The dual wavelength imaging capability allows for the visualization of functional blood parameters through an intact skull while pulse-echo ultrasound biomicroscopy images are captured simultaneously by the same scan head. The flexible hybrid design in combination with fast high-resolution imaging in 3D holds promise for generating better insights into the architecture and function of the neurovascular system.},\n bibtype = {inproceedings},\n author = {Rebling, Johannes and Estrada, Hector and Zwack, Michael and Sela, Gali and Gottschalk, Sven and Razansky, Daniel},\n editor = {Oraevsky, Alexander A. and Wang, Lihong V.},\n doi = {10.1117/12.2250635},\n booktitle = {Photons Plus Ultrasound: Imaging and Sensing 2017}\n}\n\n © 2017 SPIE. Many neurological disorders are linked to abnormal activation or pathological alterations of the vasculature in the affected brain region. Obtaining simultaneous morphological and physiological information of neurovasculature is very challenging due to the acoustic distortions and intense light scattering by the skull and brain. In addition, the size of cerebral vasculature in murine brains spans an extended range from just a few microns up to about a millimeter, all to be recorded in 3D and over an area of several dozens of mm2. Numerous imaging techniques exist that excel at characterizing certain aspects of this complex network but are only capable of providing information on a limited spatiotemporal scale. We present a hybrid ultrasound and dual-wavelength optoacoustic microscope, capable of rapid imaging of murine neurovasculature in-vivo, with high spatial resolution down to 12 μm over a large field of view exceeding 50mm2. The dual wavelength imaging capability allows for the visualization of functional blood parameters through an intact skull while pulse-echo ultrasound biomicroscopy images are captured simultaneously by the same scan head. The flexible hybrid design in combination with fast high-resolution imaging in 3D holds promise for generating better insights into the architecture and function of the neurovascular system.\n
\n\n\n\n \n\n \n \n \n \n \n \n Broadband optoacoustic characterization of cMUT and PZT transducer directivity in receive mode.\n \n \n \n \n\n\n \n Rebling, J.; Warshavski, O.; Meynier, C.; and Razansky, D.\n\n\n \n\n\n\n In Medical Imaging 2017: Ultrasonic Imaging and Tomography, volume 10139, pages 101391K, 2017. International Society for Optics and Photonics\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n\n
\n\n\n@inproceedings{\n title = {Broadband optoacoustic characterization of cMUT and PZT transducer directivity in receive mode},\n type = {inproceedings},\n year = {2017},\n keywords = {angle of incidence,bandwidth,directivity,photoacoustic,spectrum,ultrasound},\n pages = {101391K},\n volume = {10139},\n publisher = {International Society for Optics and Photonics},\n id = {99e9cd76-a9b3-3a24-9f27-9cafdc826479},\n created = {2020-07-11T11:23:47.180Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:32.875Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Optoacoustic imaging is a rapidly developing area of biomedical imaging due its combination of rich optical contrast and ultrasound depth penetration. Just like conventional pulse-echo ultrasound imaging, optoacoustic tomography relies on the use of ultrasound detector arrays with a large number of elements. The precise knowledge of the transducer's sensitivity is crucial for the prediction of its performance for a given imaging task. Sensitivity characteristics such as the central frequency and bandwidth are routinely characterized. However, this characterization is typically performed solely under normal incidence since the measurement of the angle and frequency depended sensitivity (directivity) is difficult and time consuming with existing ultrasound characterization methods. We present a simple and fast characterization method for broadband directivity measurements of the angular transducer sensitivity based on the optoacoustic effect. The method utilizes a thin absorbing suture in order to generate omnidirectional and broadband optoacoustic signals, which are calibrated using a needle hydrophone. We applied this method to characterize and compare the directivity of a conventional piezoelectric (PZT) transducer to the directivity of a capacitive micromachined ultrasonic (cMUT) transducer. Both technologies showed a similar broadband response at normal incidence and the PZT transducer displayed a more than two times larger signal to noise ratio at normal incidence. However, the cMUT transducer's sensitivity was significantly less angle-depended and outperformed the PZT's sensitivity for angles larger than 20°.},\n bibtype = {inproceedings},\n author = {Rebling, Johannes and Warshavski, Omri and Meynier, Cyril and Razansky, Daniel},\n doi = {10.1117/12.2253850},\n booktitle = {Medical Imaging 2017: Ultrasonic Imaging and Tomography}\n}\n\n © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Optoacoustic imaging is a rapidly developing area of biomedical imaging due its combination of rich optical contrast and ultrasound depth penetration. Just like conventional pulse-echo ultrasound imaging, optoacoustic tomography relies on the use of ultrasound detector arrays with a large number of elements. The precise knowledge of the transducer's sensitivity is crucial for the prediction of its performance for a given imaging task. Sensitivity characteristics such as the central frequency and bandwidth are routinely characterized. However, this characterization is typically performed solely under normal incidence since the measurement of the angle and frequency depended sensitivity (directivity) is difficult and time consuming with existing ultrasound characterization methods. We present a simple and fast characterization method for broadband directivity measurements of the angular transducer sensitivity based on the optoacoustic effect. The method utilizes a thin absorbing suture in order to generate omnidirectional and broadband optoacoustic signals, which are calibrated using a needle hydrophone. We applied this method to characterize and compare the directivity of a conventional piezoelectric (PZT) transducer to the directivity of a capacitive micromachined ultrasonic (cMUT) transducer. Both technologies showed a similar broadband response at normal incidence and the PZT transducer displayed a more than two times larger signal to noise ratio at normal incidence. However, the cMUT transducer's sensitivity was significantly less angle-depended and outperformed the PZT's sensitivity for angles larger than 20°.\n
\n\n\n\n \n\n \n \n \n \n \n \n Prediction and near-field observation of skull-guided acoustic waves.\n \n \n \n \n\n\n \n Estrada, H.; Rebling, J.; and Razansky, D.\n\n\n \n\n\n\n Physics in Medicine and Biology, 62(12): 4728-4740. 2017.\n \n\n\n\n
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\n\n\n@article{\n title = {Prediction and near-field observation of skull-guided acoustic waves},\n type = {article},\n year = {2017},\n keywords = {Guided acoustic waves,murine skull,near feld,optoacoustic,photoacoustic,ultrasound},\n pages = {4728-4740},\n volume = {62},\n id = {e915cdc7-a68c-3633-8df2-c97eccc8f0e7},\n created = {2020-07-11T11:23:47.489Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:18.628Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {Ultrasound waves propagating in water or soft biological tissue are strongly reflected when encountering the skull, which limits the use of ultrasound-based techniques in transcranial imaging and therapeutic applications. Current knowledge on the acoustic properties of the cranial bone is restricted to far-field observations, leaving its near-field unexplored. We report on the existence of skull-guided acoustic waves, which was herein confirmed by near-field measurements of optoacoustically-induced responses in ex-vivo murine skulls immersed in water. Dispersion of the guided waves was found to reasonably agree with the prediction of a multilayered flat plate model. We observed a skull-guided wave propagation over a lateral distance of at least 3 mm, with a half-decay length in the direction perpendicular to the skull ranging from 35 to 300 μm at 6 and 0.5 MHz, respectively. Propagation losses are mostly attributed to the heterogenous acoustic properties of the skull. It is generally anticipated that our findings may facilitate and broaden the application of ultrasound-mediated techniques in brain diagnostics and therapy.},\n bibtype = {article},\n author = {Estrada, Héctor and Rebling, Johannes and Razansky, Daniel},\n doi = {10.1088/1361-6560/aa63e3},\n journal = {Physics in Medicine and Biology},\n number = {12}\n}\n\n Ultrasound waves propagating in water or soft biological tissue are strongly reflected when encountering the skull, which limits the use of ultrasound-based techniques in transcranial imaging and therapeutic applications. Current knowledge on the acoustic properties of the cranial bone is restricted to far-field observations, leaving its near-field unexplored. We report on the existence of skull-guided acoustic waves, which was herein confirmed by near-field measurements of optoacoustically-induced responses in ex-vivo murine skulls immersed in water. Dispersion of the guided waves was found to reasonably agree with the prediction of a multilayered flat plate model. We observed a skull-guided wave propagation over a lateral distance of at least 3 mm, with a half-decay length in the direction perpendicular to the skull ranging from 35 to 300 μm at 6 and 0.5 MHz, respectively. Propagation losses are mostly attributed to the heterogenous acoustic properties of the skull. It is generally anticipated that our findings may facilitate and broaden the application of ultrasound-mediated techniques in brain diagnostics and therapy.\n
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\n \n \n\n \n \n \n \n \n Optoacoustic characterization of broadband directivity patterns of capacitive micromachined ultrasonic transducers.\n \n \n \n\n\n \n Rebling, J.; Warshavski, O.; Meynier, C.; and Razansky, D.\n\n\n \n\n\n\n Journal of Biomedical Optics, 22(4): 041005. 2016.\n \n\n\n\n
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\n\n\n@article{\n title = {Optoacoustic characterization of broadband directivity patterns of capacitive micromachined ultrasonic transducers},\n type = {article},\n year = {2016},\n pages = {041005},\n volume = {22},\n id = {900a5190-b1f8-3857-9714-d14e155357d1},\n created = {2020-07-11T11:23:46.818Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:46.818Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {Abstract. \\rFrequency characteristics of ultrasound detectors used in optoacoustic tomography have a major impact on imaging performance. It is common practice to select transducers based on their sensitivity at the central frequency and under normal incidence. However, the bandwidth and angular sensitivity play an equally important role in establishing the quality and accuracy of the reconstructed images. Here, we developed a calibrated optoacoustic characterization method specifically tailored for broadband measurements of the angular transducer sensitivity (directivity). Ultrawideband omnidirectional optoacoustic responses were generated by uniformly illuminating thin absorbing sutures with nanosecond laser pulses and characterized with a needle hydrophone. This calibrated optoacoustic source was used to characterize the frequency dependence of the angular response by a conventional piezoelectric transducer (PZT) and a capacitive micromachined ultrasonic transducer (cMUT) with similar size and central frequency. Furthermore, both transducers had no preamplification electronics directly attached to the detection elements. While the PZT presented a 7.8 dB sensitivity advantage at normal incidence, it was able to provide detectable signal-to-noise levels only at incidence angles of up to 20 deg whereas the cMUT maintained reasonable sensitivity levels and broadband response at incidence angles of 40 deg and beyond. We further experimentally showcase a reduction in the limited-view image artifacts resulting from the broader acceptance angle of the cMUT.},\n bibtype = {article},\n author = {Rebling, Johannes and Warshavski, Omri and Meynier, Cyril and Razansky, Daniel},\n doi = {10.1117/1.jbo.22.4.041005},\n journal = {Journal of Biomedical Optics},\n number = {4}\n}\n\n Abstract. \\rFrequency characteristics of ultrasound detectors used in optoacoustic tomography have a major impact on imaging performance. It is common practice to select transducers based on their sensitivity at the central frequency and under normal incidence. However, the bandwidth and angular sensitivity play an equally important role in establishing the quality and accuracy of the reconstructed images. Here, we developed a calibrated optoacoustic characterization method specifically tailored for broadband measurements of the angular transducer sensitivity (directivity). Ultrawideband omnidirectional optoacoustic responses were generated by uniformly illuminating thin absorbing sutures with nanosecond laser pulses and characterized with a needle hydrophone. This calibrated optoacoustic source was used to characterize the frequency dependence of the angular response by a conventional piezoelectric transducer (PZT) and a capacitive micromachined ultrasonic transducer (cMUT) with similar size and central frequency. Furthermore, both transducers had no preamplification electronics directly attached to the detection elements. While the PZT presented a 7.8 dB sensitivity advantage at normal incidence, it was able to provide detectable signal-to-noise levels only at incidence angles of up to 20 deg whereas the cMUT maintained reasonable sensitivity levels and broadband response at incidence angles of 40 deg and beyond. We further experimentally showcase a reduction in the limited-view image artifacts resulting from the broader acceptance angle of the cMUT.\n
\n\n\n\n \n\n \n \n \n \n \n \n Effects of the murine skull in optoacoustic brain microscopy.\n \n \n \n \n\n\n \n Kneipp, M.; Turner, J.; Estrada, H.; Rebling, J.; Shoham, S.; and Razansky, D.\n\n\n \n\n\n\n Journal of Biophotonics, 9(1-2): 117-123. 1 2016.\n \n\n\n\n
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\n\n\n@article{\n title = {Effects of the murine skull in optoacoustic brain microscopy},\n type = {article},\n year = {2016},\n keywords = {Microscopy,Neuroimaging,Optoacoustic technologies,Skull},\n pages = {117-123},\n volume = {9},\n websites = {http://doi.wiley.com/10.1002/jbio.201400152,http://dx.doi.org/10.1002/jbio.201400152},\n month = {1},\n publisher = {WILEY-VCH Verlag},\n id = {6b7c5a43-d58d-3a3c-b44b-6ce2faf8a9cd},\n created = {2020-07-11T11:23:47.185Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:32.022Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Article},\n private_publication = {false},\n abstract = {Despite the great promise behind the recent introduction of optoacoustic technology into the arsenal of small-animal neuroimaging methods, a variety of acoustic and light-related effects introduced by adult murine skull severely compromise the performance of optoacoustics in transcranial imaging. As a result, high-resolution noninvasive optoacoustic microscopy studies are still limited to a thin layer of pial microvasculature, which can be effectively resolved by tight focusing of the excitation light. We examined a range of distortions introduced by an adult murine skull in transcranial optoacoustic imaging under both acoustically- and optically-determined resolution scenarios. It is shown that strong low-pass filtering characteristics of the skull may significantly deteriorate the achievable spatial resolution in deep brain imaging where no light focusing is possible. While only brain vasculature with a diameter larger than 60 μm was effectively resolved via transcranial measurements with acoustic resolution, significant improvements are seen through cranial windows and thinned skull experiments. (a) Experimental setup for hybrid acoustic and optical resolution optoacoustic microscopy. (b) Transcranial scan of an adult mouse brain using the optical resolution mode. Scale bar is 375 μm. A range of distortions introduced by an adult murine skull in transcranial optoacoustic microscopy studies under both acoustically- and optically-determined resolution scenarios is examined. It is shown that strong low-pass filtering characteristics of the skull may significantly deteriorate the achievable spatial resolution in deep brain imaging where no light focusing is possible. While only brain vasculature with a diameter larger than 60 μm was effectively resolved via transcranial measurements with acoustic resolution, significant improvements are seen through cranial windows and thinned skull experiments.},\n bibtype = {article},\n author = {Kneipp, Moritz and Turner, Jake and Estrada, Héctor and Rebling, Johannes and Shoham, Shy and Razansky, Daniel},\n doi = {10.1002/jbio.201400152},\n journal = {Journal of Biophotonics},\n number = {1-2}\n}\n\n Despite the great promise behind the recent introduction of optoacoustic technology into the arsenal of small-animal neuroimaging methods, a variety of acoustic and light-related effects introduced by adult murine skull severely compromise the performance of optoacoustics in transcranial imaging. As a result, high-resolution noninvasive optoacoustic microscopy studies are still limited to a thin layer of pial microvasculature, which can be effectively resolved by tight focusing of the excitation light. We examined a range of distortions introduced by an adult murine skull in transcranial optoacoustic imaging under both acoustically- and optically-determined resolution scenarios. It is shown that strong low-pass filtering characteristics of the skull may significantly deteriorate the achievable spatial resolution in deep brain imaging where no light focusing is possible. While only brain vasculature with a diameter larger than 60 μm was effectively resolved via transcranial measurements with acoustic resolution, significant improvements are seen through cranial windows and thinned skull experiments. (a) Experimental setup for hybrid acoustic and optical resolution optoacoustic microscopy. (b) Transcranial scan of an adult mouse brain using the optical resolution mode. Scale bar is 375 μm. A range of distortions introduced by an adult murine skull in transcranial optoacoustic microscopy studies under both acoustically- and optically-determined resolution scenarios is examined. It is shown that strong low-pass filtering characteristics of the skull may significantly deteriorate the achievable spatial resolution in deep brain imaging where no light focusing is possible. While only brain vasculature with a diameter larger than 60 μm was effectively resolved via transcranial measurements with acoustic resolution, significant improvements are seen through cranial windows and thinned skull experiments.\n
\n\n\n\n \n\n \n \n \n \n \n Experimental evaluation of cMUT and PZT transducers in receive only mode for photoacoustic imaging.\n \n \n \n\n\n \n Warshavski, O.; Meynier, C.; Sénégond, N.; Chatain, P.; Rebling, J.; Razansky, D.; Felix, N.; and Nguyen-Dinh, A.\n\n\n \n\n\n\n In Photons Plus Ultrasound: Imaging and Sensing 2016, volume 9708, pages 970830, 2016. International Society for Optics and Photonics\n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Experimental evaluation of cMUT and PZT transducers in receive only mode for photoacoustic imaging},\n type = {inproceedings},\n year = {2016},\n keywords = {Array,CMUT,NEP,OA Imaging,Optoacoustic,PA Imaging,PZT,Photoacoustics,Transducers},\n pages = {970830},\n volume = {9708},\n publisher = {International Society for Optics and Photonics},\n id = {758a138a-536e-3b7c-b394-ec3fd08e3527},\n created = {2020-07-11T11:23:47.262Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.262Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© 2016 SPIE. In photoacoustic imaging, the angular reception performance of ultrasonic transducers is a critical parameter to be considered for system designers. The quantitative comparison between cMUT and PZT emphasizes the difference between the transducer requirements and specifications between conventional ultrasound and photoacoustic imaging. In this present work, we show significant benefits of cMUT based array transducers over conventional PZT arrays for the improvement of quality in photoacoustic imaging systems.},\n bibtype = {inproceedings},\n author = {Warshavski, O. and Meynier, C. and Sénégond, N. and Chatain, P. and Rebling, Johannes and Razansky, Daniel and Felix, N. and Nguyen-Dinh, A.},\n doi = {10.1117/12.2211799},\n booktitle = {Photons Plus Ultrasound: Imaging and Sensing 2016}\n}\n\n © 2016 SPIE. In photoacoustic imaging, the angular reception performance of ultrasonic transducers is a critical parameter to be considered for system designers. The quantitative comparison between cMUT and PZT emphasizes the difference between the transducer requirements and specifications between conventional ultrasound and photoacoustic imaging. In this present work, we show significant benefits of cMUT based array transducers over conventional PZT arrays for the improvement of quality in photoacoustic imaging systems.\n
\n\n\n\n \n\n \n \n \n \n \n Estimation of the skull insertion loss using an optoacoustic point source.\n \n \n \n\n\n \n Estrada, H.; Rebling, J.; Turner, J.; Kneipp, M.; Shoham, S.; and Razansky, D.\n\n\n \n\n\n\n In Photons Plus Ultrasound: Imaging and Sensing 2016, volume 9708, pages 97080M, 2016. International Society for Optics and Photonics\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n\n\n \n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@inproceedings{\n title = {Estimation of the skull insertion loss using an optoacoustic point source},\n type = {inproceedings},\n year = {2016},\n keywords = {Optoacoustic microscopy,mouse skull insertion loss,mouse skull modeling,neuroimaging,photoacoustic microscopy,transcranial brain imaging},\n pages = {97080M},\n volume = {9708},\n publisher = {International Society for Optics and Photonics},\n id = {016cc269-4dc8-399e-a282-d32c85a61353},\n created = {2020-07-11T11:23:47.327Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.327Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Conference Proceedings},\n private_publication = {false},\n abstract = {© 2016 SPIE. The acoustically-mismatched skull bone poses significant challenges for the application of ultrasonic and optical techniques in neuroimaging, still typically requiring invasive approaches using craniotomy or skull thinning. Optoacoustic imaging partially circumvents the acoustic distortions due to the skull because the induced wave is transmitted only once as opposed to the round trip in pulse-echo ultrasonography. To this end, the mouse brain has been successfully imaged transcranially by optoacoustic scanning microscopy. Yet, the skull may adversely affect the lateral and axial resolution of transcranial brain images. In order to accurately characterize the complex behavior of the optoacoustic signal as it traverses through the skull, one needs to consider the ultrawideband nature of the optoacoustic signals. Here the insertion loss of murine skull has been measured by means of a hybrid optoacoustic-ultrasound scanning microscope having a spherically focused PVDF transducer and pulsed laser excitation at 532 nm of a 20 μm diameter absorbing microsphere acting as an optoacoustic point source. Accurate modeling of the acoustic transmission through the skull is further performed using a Fourier-domain expansion of a solid-plate model, based on the simultaneously acquired pulse-echo ultrasound image providing precise information about the skull's position and its orientation relative to the optoacoustic source. Good qualitative agreement has been found between the a solid-plate model and experimental measurements. The presented strategy might pave the way for modeling skull effects and deriving efficient correction schemes to account for acoustic distortions introduced by an adult murine skull, thus improving the spatial resolution, effective penetration depth and overall image quality of transcranial optoacoustic brain microscopy.},\n bibtype = {inproceedings},\n author = {Estrada, Héctor and Rebling, Johannes and Turner, Jake and Kneipp, Moritz and Shoham, Shy and Razansky, Daniel},\n doi = {10.1117/12.2211183},\n booktitle = {Photons Plus Ultrasound: Imaging and Sensing 2016}\n}\n\n © 2016 SPIE. The acoustically-mismatched skull bone poses significant challenges for the application of ultrasonic and optical techniques in neuroimaging, still typically requiring invasive approaches using craniotomy or skull thinning. Optoacoustic imaging partially circumvents the acoustic distortions due to the skull because the induced wave is transmitted only once as opposed to the round trip in pulse-echo ultrasonography. To this end, the mouse brain has been successfully imaged transcranially by optoacoustic scanning microscopy. Yet, the skull may adversely affect the lateral and axial resolution of transcranial brain images. In order to accurately characterize the complex behavior of the optoacoustic signal as it traverses through the skull, one needs to consider the ultrawideband nature of the optoacoustic signals. Here the insertion loss of murine skull has been measured by means of a hybrid optoacoustic-ultrasound scanning microscope having a spherically focused PVDF transducer and pulsed laser excitation at 532 nm of a 20 μm diameter absorbing microsphere acting as an optoacoustic point source. Accurate modeling of the acoustic transmission through the skull is further performed using a Fourier-domain expansion of a solid-plate model, based on the simultaneously acquired pulse-echo ultrasound image providing precise information about the skull's position and its orientation relative to the optoacoustic source. Good qualitative agreement has been found between the a solid-plate model and experimental measurements. The presented strategy might pave the way for modeling skull effects and deriving efficient correction schemes to account for acoustic distortions introduced by an adult murine skull, thus improving the spatial resolution, effective penetration depth and overall image quality of transcranial optoacoustic brain microscopy.\n
\n\n\n\n \n\n \n \n \n \n \n Broadband acoustic properties of a murine skull.\n \n \n \n\n\n \n Estrada, H.; Rebling, J.; Turner, J.; and Razansky, D.\n\n\n \n\n\n\n Physics in Medicine and Biology, 61(5): 1932-1946. 2016.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Broadband acoustic properties of a murine skull},\n type = {article},\n year = {2016},\n keywords = {acoustic wave propagation in solids,neuroimaging,optoacoustic imaging,photoacoustics,skull characterization,ultrasound imaging},\n pages = {1932-1946},\n volume = {61},\n id = {43cfd997-7e76-3edb-95d8-a4a7982ea31f},\n created = {2020-07-11T11:23:47.556Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:23:47.556Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {It has been well recognized that the presence of a skull imposes harsh restrictions on the use of ultrasound and optoacoustic techniques in the study, treatment and modulation of the brain function. We propose a rigorous modeling and experimental methodology for estimating the insertion loss and the elastic constants of the skull over a wide range of frequencies and incidence angles. A point-source-like excitation of ultrawideband acoustic radiation was induced via the absorption of nanosecond duration laser pulses by a 20 μm diameter microsphere. The acoustic waves transmitted through the skull are recorded by a broadband, spherically focused ultrasound transducer. A coregistered pulse-echo ultrasound scan is subsequently performed to provide accurate skull geometry to be fed into an acoustic transmission model represented in an angular spectrum domain. The modeling predictions were validated by measurements taken from a glass cover-slip and ex vivo adult mouse skulls. The flexible semi-analytical formulation of the model allows for seamless extension to other transducer geometries and diverse experimental scenarios involving broadband acoustic transmission through locally flat solid structures. It is anticipated that accurate quantification and modeling of the skull transmission effects would ultimately allow for skull aberration correction in a broad variety of applications employing transcranial detection or transmission of high frequency ultrasound.},\n bibtype = {article},\n author = {Estrada, Héctor and Rebling, Johannes and Turner, Jake and Razansky, Daniel},\n doi = {10.1088/0031-9155/61/5/1932},\n journal = {Physics in Medicine and Biology},\n number = {5}\n}\n\n It has been well recognized that the presence of a skull imposes harsh restrictions on the use of ultrasound and optoacoustic techniques in the study, treatment and modulation of the brain function. We propose a rigorous modeling and experimental methodology for estimating the insertion loss and the elastic constants of the skull over a wide range of frequencies and incidence angles. A point-source-like excitation of ultrawideband acoustic radiation was induced via the absorption of nanosecond duration laser pulses by a 20 μm diameter microsphere. The acoustic waves transmitted through the skull are recorded by a broadband, spherically focused ultrasound transducer. A coregistered pulse-echo ultrasound scan is subsequently performed to provide accurate skull geometry to be fed into an acoustic transmission model represented in an angular spectrum domain. The modeling predictions were validated by measurements taken from a glass cover-slip and ex vivo adult mouse skulls. The flexible semi-analytical formulation of the model allows for seamless extension to other transducer geometries and diverse experimental scenarios involving broadband acoustic transmission through locally flat solid structures. It is anticipated that accurate quantification and modeling of the skull transmission effects would ultimately allow for skull aberration correction in a broad variety of applications employing transcranial detection or transmission of high frequency ultrasound.\n
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\n\n \n \n 2015\n \n \n (1)\n \n \n
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\n \n \n\n \n \n \n \n \n \n Short and long-term phototoxicity in cells expressing genetic reporters under nanosecond laser exposure.\n \n \n \n \n\n\n \n Gottschalk, S.; Estrada, H.; Degtyaruk, O.; Rebling, J.; Klymenko, O.; Rosemann, M.; and Razansky, D.\n\n\n \n\n\n\n Biomaterials, 69: 38-44. 2015.\n \n\n\n\n
\n\n\n\n \n \nPaper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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\n\n\n@article{\n title = {Short and long-term phototoxicity in cells expressing genetic reporters under nanosecond laser exposure},\n type = {article},\n year = {2015},\n keywords = {Fluorescent protein,Nanosecond laser pulses,Optoacoustic imaging,Photobleaching,Phototoxicity},\n pages = {38-44},\n volume = {69},\n id = {e1d3bcdc-b9e5-3de9-911c-474a1619c7c8},\n created = {2020-07-11T11:23:46.664Z},\n file_attached = {true},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:24:22.330Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n private_publication = {false},\n abstract = {Nanosecond-duration laser pulses are exploited in a plethora of therapeutic and diagnostic applications, such as optoacoustic imaging. However, phototoxicity effects of pulsed radiation in living cells, in particular those expressing genetic reporters, are not well understood. We established a three-dimensional fluorescent protein expressing cellular model in order to reliably investigate the extent and major exposure parameters responsible for both photobleaching and phototoxicity under pulsed laser exposure, unveiling a variety of possible effects on living cells, from reversible photobleaching to cytotoxicity and cell death. Significant losses of fluorescence levels were identified when exposing the cells to illumination conditions considered safe under common standards for skin exposure in diagnostic imaging applications. Thus, the use of photolabile fluorescent proteins and their in vivo exposure parameters have to be designed carefully for all applications using pulsed nanosecond radiation. In particular, loss of signal due to bleaching may significantly alter signals in longitudinal measurements, making data quantification challenging.},\n bibtype = {article},\n author = {Gottschalk, Sven and Estrada, Héctor and Degtyaruk, Oleksiy and Rebling, Johannes and Klymenko, Olena and Rosemann, Michael and Razansky, Daniel},\n doi = {10.1016/j.biomaterials.2015.07.051},\n journal = {Biomaterials}\n}\n\n Nanosecond-duration laser pulses are exploited in a plethora of therapeutic and diagnostic applications, such as optoacoustic imaging. However, phototoxicity effects of pulsed radiation in living cells, in particular those expressing genetic reporters, are not well understood. We established a three-dimensional fluorescent protein expressing cellular model in order to reliably investigate the extent and major exposure parameters responsible for both photobleaching and phototoxicity under pulsed laser exposure, unveiling a variety of possible effects on living cells, from reversible photobleaching to cytotoxicity and cell death. Significant losses of fluorescence levels were identified when exposing the cells to illumination conditions considered safe under common standards for skin exposure in diagnostic imaging applications. Thus, the use of photolabile fluorescent proteins and their in vivo exposure parameters have to be designed carefully for all applications using pulsed nanosecond radiation. In particular, loss of signal due to bleaching may significantly alter signals in longitudinal measurements, making data quantification challenging.\n
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\n\n \n \n 2013\n \n \n (1)\n \n \n
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\n \n \n\n \n \n \n \n \n \n Chirped-pulse and cavity-based fourier transform microwave spectroscopy of a chiral epoxy ester: Methyl glycidate.\n \n \n \n \n\n\n \n Thomas, J.; Yiu, J.; Rebling, J.; Jäger, W.; and Xu, Y.\n\n\n \n\n\n\n Journal of Physical Chemistry A, 117(50): 13249-13254. 12 2013.\n \n\n\n\n
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\n\n\n@article{\n title = {Chirped-pulse and cavity-based fourier transform microwave spectroscopy of a chiral epoxy ester: Methyl glycidate},\n type = {article},\n year = {2013},\n pages = {13249-13254},\n volume = {117},\n websites = {http://pubs.acs.org/doi/10.1021/jp402552t},\n month = {12},\n day = {19},\n id = {6916eed1-0609-3d06-a0cd-19df96bc3fdb},\n created = {2019-03-13T14:00:29.470Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:16:06.780Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Rotational spectra of a chiral epoxy ester, methyl glycidate, were measured using a chirped-pulse and a cavity-based Fourier transform microwave spectrometer. The two lowest energy conformers where the epoxy oxygen and the ester oxygen atoms are in the syn and anti relative orientation with respect to each other were identified experimentally. Spectra of four 13C isotopologues of the lowest energy conformer of methyl glycidate were also measured and assigned. All of the observed rotational transitions are split into doublets due to the presence of the ester methyl internal rotor. The rotational constants and the internal rotation barrier height for the ester methyl group were determined for both conformers of methyl glycidate and for the four 13C isotopologues of the most stable conformer. A value for the interconversion barrier between the two most stable conformers was estimated. Furthermore, comparison to strawberry aldehyde, a larger derivative of methyl glycidate, shows how the syn-anti conformational equilibrium shifts as a result of the additional bulky substituents at the epoxy ring and at the ester oxygen atom. © 2013 American Chemical Society.},\n bibtype = {article},\n author = {Thomas, Javix and Yiu, Jensen and Rebling, Johannes and Jäger, Wolfgang and Xu, Yunjie},\n doi = {10.1021/jp402552t},\n journal = {Journal of Physical Chemistry A},\n number = {50}\n}\n\n Rotational spectra of a chiral epoxy ester, methyl glycidate, were measured using a chirped-pulse and a cavity-based Fourier transform microwave spectrometer. The two lowest energy conformers where the epoxy oxygen and the ester oxygen atoms are in the syn and anti relative orientation with respect to each other were identified experimentally. Spectra of four 13C isotopologues of the lowest energy conformer of methyl glycidate were also measured and assigned. All of the observed rotational transitions are split into doublets due to the presence of the ester methyl internal rotor. The rotational constants and the internal rotation barrier height for the ester methyl group were determined for both conformers of methyl glycidate and for the four 13C isotopologues of the most stable conformer. A value for the interconversion barrier between the two most stable conformers was estimated. Furthermore, comparison to strawberry aldehyde, a larger derivative of methyl glycidate, shows how the syn-anti conformational equilibrium shifts as a result of the additional bulky substituents at the epoxy ring and at the ester oxygen atom. © 2013 American Chemical Society.\n
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\n\n\n\n\n \n \n 2012\n \n \n (2)\n \n \n
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\n \n \n\n \n \n \n \n \n \n Observation of vector solitons with hidden vorticity.\n \n \n \n \n\n\n \n Izdebskaya, Y., V.; Rebling, J.; Desyatnikov, A., S.; and Kivshar, Y., S.\n\n\n \n\n\n\n Optics Letters, 37(5): 767. 3 2012.\n \n\n\n\n
\n\n\n\n \n \nWebsite\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n\n
\n\n\n@article{\n title = {Observation of vector solitons with hidden vorticity},\n type = {article},\n year = {2012},\n pages = {767},\n volume = {37},\n websites = {https://www.osapublishing.org/abstract.cfm?URI=ol-37-5-767},\n month = {3},\n day = {1},\n id = {a5737023-9689-3849-851f-c23fbfdaec7a},\n created = {2019-03-13T14:00:29.368Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:16:06.911Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {This letter reports the first experimental observation, to our knowledge, of optical vector solitons composed of two incoherently coupled vortex components. We employ nematic liquid crystal to generate stable vector solitons with counterrotating vortices and hidden vorticity. In contrast, the solitons with explicit vorticity and corotating vortex components show azimuthal splitting.},\n bibtype = {article},\n author = {Izdebskaya, Yana V. and Rebling, Johannes and Desyatnikov, Anton S. and Kivshar, Yuri S.},\n doi = {10.1364/ol.37.000767},\n journal = {Optics Letters},\n number = {5}\n}\n\n This letter reports the first experimental observation, to our knowledge, of optical vector solitons composed of two incoherently coupled vortex components. We employ nematic liquid crystal to generate stable vector solitons with counterrotating vortices and hidden vorticity. In contrast, the solitons with explicit vorticity and corotating vortex components show azimuthal splitting.\n
\n\n\n\n \n\n \n \n \n \n \n \n All-optical switching of a signal by a pair of interacting nematicons.\n \n \n \n \n\n\n \n Izdebskaya, Y., V.; Rebling, J.; Desyatnikov, A., S.; Assanto, G.; and Kivshar, Y., S.\n\n\n \n\n\n\n Optics Express, 20(22): 24701. 10 2012.\n \n\n\n\n
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\n\n\n@article{\n title = {All-optical switching of a signal by a pair of interacting nematicons},\n type = {article},\n year = {2012},\n pages = {24701},\n volume = {20},\n websites = {https://www.osapublishing.org/oe/abstract.cfm?uri=oe-20-22-24701},\n month = {10},\n day = {22},\n id = {b6344fe0-3275-3c75-8c7e-b2308fcf440b},\n created = {2019-03-13T14:00:29.472Z},\n file_attached = {false},\n profile_id = {324e2dd8-ad33-3b22-a1f1-eb499a0e8419},\n last_modified = {2020-07-11T11:16:06.878Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {We investigate a power tunable junction formed by two interacting spatial solitons self-trapped in nematic liquid crystals. By launching a counter-propagating copolarized probe we assess the guided-wave behavior induced by the solitons and demonstrate a novel all-optical switch. Varying soliton power the probe gets trapped into one or two or three guided-waves by the soliton-induced index perturbation, an effect supported by the nonlocal nonlinearity. © 2012 Optical Society of America.},\n bibtype = {article},\n author = {Izdebskaya, Ya. V. and Rebling, J. and Desyatnikov, A. S. and Assanto, G. and Kivshar, Yu. S.},\n doi = {10.1364/oe.20.024701},\n journal = {Optics Express},\n number = {22}\n}\n\n We investigate a power tunable junction formed by two interacting spatial solitons self-trapped in nematic liquid crystals. By launching a counter-propagating copolarized probe we assess the guided-wave behavior induced by the solitons and demonstrate a novel all-optical switch. Varying soliton power the probe gets trapped into one or two or three guided-waves by the soliton-induced index perturbation, an effect supported by the nonlocal nonlinearity. © 2012 Optical Society of America.\n
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