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\n \n 2020\n \n \n (4)\n \n \n

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\n \n\n \n \n \n \n \n \n Profiling the transcriptome response of Atlantic salmon head kidney to formalin-killed Renibacterium salmoninarum.\n \n \n \n \n\n\n \n Eslamloo, K.; Kumar, S.; Caballero-Solares, A.; Gnanagobal, H.; Santander, J.; and Rise, M., L.\n\n\n \n\n\n\n Fish & Shellfish Immunology, 98: 937-949. 3 2020.\n \n\n\n\n
\n\n\n\n \n \n $\"Profiling$ Paper\n \n \n \n $\"Profiling$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@article{\n title = {Profiling the transcriptome response of Atlantic salmon head kidney to formalin-killed Renibacterium salmoninarum},\n type = {article},\n year = {2020},\n pages = {937-949},\n volume = {98},\n websites = {https://www.sciencedirect.com/science/article/pii/S1050464819311003},\n month = {3},\n publisher = {Academic Press},\n day = {1},\n id = {f13083cd-61be-33ee-8f8b-bafd7fe246a1},\n created = {2020-11-18T19:53:58.790Z},\n accessed = {2020-07-14},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:05.459Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {false},\n hidden = {false},\n private_publication = {false},\n abstract = {Renibacterium salmoninarum is a Gram-positive, intracellular bacterial pathogen that causes Bacterial Kidney Disease (BKD) in Atlantic salmon (Salmo salar). The host transcriptomic response to this immune-suppressive pathogen remains poorly understood. To identify R. salmoninarum-responsive genes, Atlantic salmon were intraperitoneally injected with a low (5 × 105 cells/kg, Low-Rs) or high (5 × 107 cells/kg; High-Rs) dose of formalin-killed R. salmoninarum bacterin or phosphate-buffered saline (PBS control); head kidney samples were collected before and 24 h after injection. Using 44K microarray analysis, we identified 107 and 345 differentially expressed probes in response to R. salmoninarum bacterin (i.e. High-Rs vs. PBS control) by Significance Analysis of Microarrays (SAM) and Rank Products (RP), respectively. Twenty-two microarray-identified genes were subjected to qPCR assays, and 17 genes were confirmed as being significantly responsive to the bacterin. There was an up-regulation in expression of genes playing putative roles as immune receptors and antimicrobial effectors. Genes with putative roles as pathogen recognition (e.g. clec12b and tlr5) or immunoregulatory (e.g. tnfrsf6b and tnfrsf11b) receptors were up-regulated in response to R. salmoninarum bacterin. Also, chemokines and a chemokine receptor showed opposite regulation [up-regulation of effectors (i.e. ccl13 and ccl) and down-regulation of cxcr1] in response to the bacterin. The present study identified and validated novel biomarker genes (e.g. ctsl1, lipe, cldn4, ccny) that can be used to assess Atlantic salmon response to R. salmoninarum, and will be valuable in the development of tools to combat BKD.},\n bibtype = {article},\n author = {Eslamloo, Khalil and Kumar, Surendra and Caballero-Solares, Albert and Gnanagobal, Hajarooba and Santander, Javier and Rise, Matthew L.},\n doi = {10.1016/J.FSI.2019.11.057},\n journal = {Fish & Shellfish Immunology}\n}

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\n \n\n \n \n \n \n \n \n Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria).\n \n \n \n \n\n\n \n Vasquez, I.; Cao, T.; Hossain, A.; Valderrama, K.; Gnanagobal, H.; Dang, M.; Leeuwis, R., H.; Ness, M.; Campbell, B.; Gendron, R.; Kao, K.; Westcott, J.; Gamperl, A., K.; and Santander, J.\n\n\n \n\n\n\n Fish & Shellfish Immunology, 104: 557-566. 9 2020.\n \n\n\n\n
\n\n\n\n \n \n $\"Aeromonas$ Paper\n \n \n \n $\"Aeromonas$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@article{\n title = {Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria)},\n type = {article},\n year = {2020},\n pages = {557-566},\n volume = {104},\n websites = {https://www.sciencedirect.com/science/article/pii/S1050464820304204,https://linkinghub.elsevier.com/retrieve/pii/S1050464820304204},\n month = {9},\n publisher = {Academic Press},\n day = {1},\n id = {30cdd9ef-dca2-3071-992b-669fc6aaf59a},\n created = {2020-11-18T19:53:58.796Z},\n accessed = {2020-07-14},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:09.937Z},\n read = {true},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Effective vaccine programs against Aeromonas salmonicida have been identified as a high priority area for the sablefish (Anoplopoma fimbria) aquaculture. In this study, we established an A. salmonicida infection model in sablefish to evaluate the efficacy of commercial vaccines and an autogenous vaccine preparation. Groups of 40 fish were intraperitoneally (ip) injected with different doses of A. salmonicida J410 isolated from infected sablefish to calculate the median lethal dose (LD50). Samples of blood, head kidney, spleen, brain, and liver were also collected at different time points to determine the infection kinetics. The LD50 was estimated as ~3 × 105 CFU/dose. To evaluate the immune protection provided by an autogenous vaccine and two commercial vaccines in a common garden experimental design, 140 fish were PIT-tagged, vaccinated and distributed equally into 4 tanks (35 fish for each group, including a control group). Blood samples were taken every 2 weeks to evaluate IgM titers. At 10 weeks post-immunization, all groups were ip challenged with 100 times the calculated LD50 for A. salmonicida J410. A. salmonicida was detected after 5 days post-infection (dpi) in all collected tissues. At 30 days post-challenge the relative percentage survival (RPS) with respect to the control group was calculated for each vaccine. The RPS for the bacterin mix was 65.22%, for Forte Micro 4® vaccine was 56.52% and for Alpha Ject Micro 4® was 30.43%, and these RPS values were reflected by A. salmonicida tissue colonization levels at 10 days post-challenge. Total IgM titers peaked at 6–8 weeks post-immunization, where the autogenous vaccine group showed the highest IgM titers and these values were consistent with the RPS data. Also, we determined that the A. salmonicida A-layer binds to immunoglobulins F(ab)' in a non-specific fashion, interfering with immune assays and potentially vaccine efficacy. Our results indicate that vaccine design influences sablefish immunity and provide a guide for future sablefish vaccine programs.},\n bibtype = {article},\n author = {Vasquez, Ignacio and Cao, Trung and Hossain, Ahmed and Valderrama, Katherinne and Gnanagobal, Hajarooba and Dang, My and Leeuwis, Robine H.J. and Ness, Michael and Campbell, Briony and Gendron, Robert and Kao, Kenneth and Westcott, Jillian and Gamperl, A. Kurt and Santander, Javier},\n doi = {10.1016/j.fsi.2020.06.005},\n journal = {Fish & Shellfish Immunology}\n}

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\n Effective vaccine programs against Aeromonas salmonicida have been identified as a high priority area for the sablefish (Anoplopoma fimbria) aquaculture. In this study, we established an A. salmonicida infection model in sablefish to evaluate the efficacy of commercial vaccines and an autogenous vaccine preparation. Groups of 40 fish were intraperitoneally (ip) injected with different doses of A. salmonicida J410 isolated from infected sablefish to calculate the median lethal dose (LD50). Samples of blood, head kidney, spleen, brain, and liver were also collected at different time points to determine the infection kinetics. The LD50 was estimated as ~3 × 105 CFU/dose. To evaluate the immune protection provided by an autogenous vaccine and two commercial vaccines in a common garden experimental design, 140 fish were PIT-tagged, vaccinated and distributed equally into 4 tanks (35 fish for each group, including a control group). Blood samples were taken every 2 weeks to evaluate IgM titers. At 10 weeks post-immunization, all groups were ip challenged with 100 times the calculated LD50 for A. salmonicida J410. A. salmonicida was detected after 5 days post-infection (dpi) in all collected tissues. At 30 days post-challenge the relative percentage survival (RPS) with respect to the control group was calculated for each vaccine. The RPS for the bacterin mix was 65.22%, for Forte Micro 4® vaccine was 56.52% and for Alpha Ject Micro 4® was 30.43%, and these RPS values were reflected by A. salmonicida tissue colonization levels at 10 days post-challenge. Total IgM titers peaked at 6–8 weeks post-immunization, where the autogenous vaccine group showed the highest IgM titers and these values were consistent with the RPS data. Also, we determined that the A. salmonicida A-layer binds to immunoglobulins F(ab)' in a non-specific fashion, interfering with immune assays and potentially vaccine efficacy. Our results indicate that vaccine design influences sablefish immunity and provide a guide for future sablefish vaccine programs.\n

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\n \n\n \n \n \n \n \n \n Effects of Vitamin D2 (Ergocalciferol) and D3 (Cholecalciferol) on Atlantic Salmon (Salmo salar) Primary Macrophage Immune Response to Aeromonas salmonicida subsp. salmonicida Infection.\n \n \n \n \n\n\n \n Soto-Dávila, M.; Valderrama, K.; Inkpen, S., M.; Hall, J., R.; Rise, M., L.; and Santander, J.\n\n\n \n\n\n\n Frontiers in Immunology, 10(January): 1-14. 2020.\n \n\n\n\n
\n\n\n\n \n \n $\"Effects$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Effects of Vitamin D2 (Ergocalciferol) and D3 (Cholecalciferol) on Atlantic Salmon (Salmo salar) Primary Macrophage Immune Response to Aeromonas salmonicida subsp. salmonicida Infection},\n type = {article},\n year = {2020},\n keywords = {Aeromonas salmonicida,Atlantic salmon,Gram-negative,innate immunity,primary macrophages,vitamin D2,vitamin D3},\n pages = {1-14},\n volume = {10},\n id = {859df34b-8d2f-3c7e-ac12-81d56ca5b5d3},\n created = {2020-11-18T19:53:58.977Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:12.953Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) are fat-soluble secosteroid hormones obtained from plant and animal sources, respectively. Fish incorporates vitamin D2 and D3 through the diet. In mammals, vitamin D forms are involved in mineral metabolism, cell growth, tissue differentiation, and antibacterial immune response. Vitamin D is an essential nutrient in aquafeeds for finfish. However, the influence of vitamin D on fish cell immunity has not yet been explored. Here, we examined the effects of vitamin D2 and vitamin D3 on Salmo salar primary macrophage immune response to A. salmonicida subspecies salmonicida infection under in vitro conditions. We determined that high concentrations of vitamin D2 (100,000 ng/ml) and D3 (10,000 ng/ml) affect the growth of A. salmonicida and decrease the viability of S. salar primary macrophages. In addition, we determined that primary macrophages pre-treated with a biologically relevant concentration of vitamin D3 for 24 h showed a decrease of A. salmonicida infection. In contrast, vitamin D2 did not influence the antibacterial activity of the S. salar macrophages infected with A. salmonicida. Vitamin D2 and D3 did not influence the expression of canonical genes related to innate immune response. On the other hand, we found that A. salmonicida up-regulated the expression of several canonical genes and suppressed the expression of leukocyte-derived chemotaxin 2 (lect-2) gene, involved in neutrophil recruitment. Primary macrophages pre-treated for 24 h with vitamin D3 counteracted this immune suppression and up-regulated the transcription of lect-2. Our results suggest that vitamin D3 affects A. salmonicida attachment to the S. salar primary macrophages, and as a consequence, the A. salmonicida invasion decreased. Moreover, our study shows that the positive effects of vitamin D3 on fish cell immunity seem to be related to the lect-2 innate immunity mechanisms. We did not identify positive effects of vitamin D2 on fish cell immunity. In conclusion, we determined that the inactive form of vitamin D3, cholecalciferol, induced anti-bacterial innate immunity pathways in Atlantic salmon primary macrophages, suggesting that its utilization as a component of a healthy aquafeed diet in Atlantic salmon could enhance the immune response against A. salmonicida.},\n bibtype = {article},\n author = {Soto-Dávila, Manuel and Valderrama, Katherinne and Inkpen, Sabrina M. and Hall, Jennifer R. and Rise, Matthew L. and Santander, Javier},\n doi = {10.3389/fimmu.2019.03011},\n journal = {Frontiers in Immunology},\n number = {January}\n}

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\n Vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol) are fat-soluble secosteroid hormones obtained from plant and animal sources, respectively. Fish incorporates vitamin D2 and D3 through the diet. In mammals, vitamin D forms are involved in mineral metabolism, cell growth, tissue differentiation, and antibacterial immune response. Vitamin D is an essential nutrient in aquafeeds for finfish. However, the influence of vitamin D on fish cell immunity has not yet been explored. Here, we examined the effects of vitamin D2 and vitamin D3 on Salmo salar primary macrophage immune response to A. salmonicida subspecies salmonicida infection under in vitro conditions. We determined that high concentrations of vitamin D2 (100,000 ng/ml) and D3 (10,000 ng/ml) affect the growth of A. salmonicida and decrease the viability of S. salar primary macrophages. In addition, we determined that primary macrophages pre-treated with a biologically relevant concentration of vitamin D3 for 24 h showed a decrease of A. salmonicida infection. In contrast, vitamin D2 did not influence the antibacterial activity of the S. salar macrophages infected with A. salmonicida. Vitamin D2 and D3 did not influence the expression of canonical genes related to innate immune response. On the other hand, we found that A. salmonicida up-regulated the expression of several canonical genes and suppressed the expression of leukocyte-derived chemotaxin 2 (lect-2) gene, involved in neutrophil recruitment. Primary macrophages pre-treated for 24 h with vitamin D3 counteracted this immune suppression and up-regulated the transcription of lect-2. Our results suggest that vitamin D3 affects A. salmonicida attachment to the S. salar primary macrophages, and as a consequence, the A. salmonicida invasion decreased. Moreover, our study shows that the positive effects of vitamin D3 on fish cell immunity seem to be related to the lect-2 innate immunity mechanisms. We did not identify positive effects of vitamin D2 on fish cell immunity. In conclusion, we determined that the inactive form of vitamin D3, cholecalciferol, induced anti-bacterial innate immunity pathways in Atlantic salmon primary macrophages, suggesting that its utilization as a component of a healthy aquafeed diet in Atlantic salmon could enhance the immune response against A. salmonicida.\n

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\n \n\n \n \n \n \n \n \n Transcriptomic profiling in fins of atlantic salmon parasitized with sea lice: evidence for an early imbalance between chalimus-induced immunomodulation and the host’s defense response.\n \n \n \n \n\n\n \n Umasuthan, N.; Xue, X.; Caballero-Solares, A.; Kumar, S.; Westcott, J., D.; Chen, Z.; Fast, M., D.; Skugor, S.; Nowak, B., F.; Taylor, R., G.; and Rise, M., L.\n\n\n \n\n\n\n International Journal of Molecular Sciences, 21(7). 2020.\n \n\n\n\n
\n\n\n\n \n \n $\"Transcriptomic$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Transcriptomic profiling in fins of atlantic salmon parasitized with sea lice: evidence for an early imbalance between chalimus-induced immunomodulation and the host’s defense response},\n type = {article},\n year = {2020},\n keywords = {44K microarray,Anti-sea lice response,Chalimus,Fin transcriptome,Immunogenomics,Immunomodulation,Lepeophtheirus salmonis,Salmo salar},\n volume = {21},\n id = {2cb59951-1003-38a2-a632-c86c97f55eaf},\n created = {2020-11-18T19:53:59.191Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:23.384Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Parasitic sea lice (e.g., Lepeophtheirus salmonis) cause costly outbreaks in salmon farming. Molecular insights into parasite-induced host responses will provide the basis for improved management strategies. We investigated the early transcriptomic responses in pelvic fins of Atlantic salmon parasitized with chalimus I stage sea lice. Fin samples collected from non-infected (i.e. pre-infected) control (PRE) and at chalimus-attachment sites (ATT) and adjacent to chalimus-attachment sites (ADJ) from infected fish were used in profiling global gene expression using 44 K microarrays. We identified 6568 differentially expressed probes (DEPs, FDR < 5%) that included 1928 shared DEPs between ATT and ADJ compared to PRE. The ATT versus ADJ comparison revealed 90 DEPs, all of which were upregulated in ATT samples. Gene ontology/pathway term network analyses revealed profound changes in physiological processes, including extracellular matrix (ECM) degradation, tissue repair/remodeling and wound healing, immunity and defense, chemotaxis and signaling, antiviral response, and redox homeostasis in infected fins. The QPCR analysis of 37 microarray-identified transcripts representing these functional themes served to confirm the microarray results with a significant positive correlation (p < 0.0001). Most immune/defense-relevant transcripts were downregulated in both ATT and ADJ sites compared to PRE, suggesting that chalimus exerts immunosuppressive effects in the salmon’s fins. The comparison between ATT and ADJ sites demonstrated the upregulation of a suite of immune-relevant transcripts, evidencing the salmon’s attempt to mount an anti-lice response. We hypothesize that an imbalance between immunomodulation caused by chalimus during the early phase of infection and weak defense response manifested by Atlantic salmon makes it a susceptible host for L. salmonis.},\n bibtype = {article},\n author = {Umasuthan, Navaneethaiyer and Xue, Xi and Caballero-Solares, Albert and Kumar, Surendra and Westcott, Jillian D. and Chen, Zhiyu and Fast, Mark D. and Skugor, Stanko and Nowak, Barbara F. and Taylor, Richard G. and Rise, Matthew L.},\n doi = {10.3390/ijms21072417},\n journal = {International Journal of Molecular Sciences},\n number = {7}\n}

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\n \n 2019\n \n \n (6)\n \n \n

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\n \n\n \n \n \n \n \n \n Characterization and transcript expression analyses of Atlantic cod viperin.\n \n \n \n \n\n\n \n Eslamloo, K.; Ghorbani, A.; Xue, X.; Inkpen, S., M.; Larijani, M.; and Rise, M., L.\n\n\n \n\n\n\n Frontiers in Immunology, 10(MAR). 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"Characterization$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Characterization and transcript expression analyses of Atlantic cod viperin},\n type = {article},\n year = {2019},\n keywords = {DsRNA,Gadus morhua,Inhibition of antiviral responses,QPCR,Rsad2,Teleost ISGs},\n volume = {10},\n id = {b27823e0-0795-3fe8-a609-6e14e47e9657},\n created = {2020-11-18T19:53:58.378Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:03.866Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Viperin is a key antiviral effector in immune responses of vertebrates including the Atlantic cod (Gadus morhua). Using cloning, sequencing and gene expression analyses, we characterized the Atlantic cod viperin at the nucleotide and hypothetical amino acid levels, and its regulating factors were investigated. Atlantic cod viperin cDNA is 1,342 bp long, and its predicted protein contains 347 amino acids. Using in silico analyses, we showed that Atlantic cod viperin is composed of 5 exons, as in other vertebrate orthologs. In addition, the radical SAM domain and C-terminal sequences of the predicted Viperin protein are highly conserved among various species. As expected, Atlantic cod Viperin was most closely related to other teleost orthologs. Using computational modeling, we show that the Atlantic cod Viperin forms similar overall protein architecture compared to mammalian Viperins. qPCR revealed that viperin is a weakly expressed transcript during embryonic development of Atlantic cod. In adults, the highest constitutive expression of viperin transcript was found in blood compared with 18 other tissues. Using isolated macrophages and synthetic dsRNA (pIC) stimulation, we tested various immune inhibitors to determine the possible regulating pathways of Atlantic cod viperin. Atlantic cod viperin showed a comparable pIC induction to other well-known antiviral genes (e.g., interferon gamma and interferon-stimulated gene 15-1) in response to various immune inhibitors. The pIC induction of Atlantic cod viperin was significantly inhibited with 2-Aminopurine, Chloroquine, SB202190, and Ruxolitinib. Therefore, endosomal-TLR-mediated pIC recognition and signal transducers (i.e., PKR and p38 MAPK) downstream of the TLR-dependent pathway may activate the gene expression response of Atlantic cod viperin. Also, these results suggest that antiviral responses of Atlantic cod viperin may be transcriptionally regulated through the interferon-activated pathway.},\n bibtype = {article},\n author = {Eslamloo, Khalil and Ghorbani, Atefeh and Xue, Xi and Inkpen, Sabrina M. and Larijani, Mani and Rise, Matthew L.},\n doi = {10.3389/fimmu.2019.00311},\n journal = {Frontiers in Immunology},\n number = {MAR}\n}

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\n \n\n \n \n \n \n \n \n A Novel 3-Dimensional Co-culture Method Reveals a Partial Mesenchymal to Epithelial Transition in Breast Cancer Cells Induced by Adipocytes.\n \n \n \n \n\n\n \n Pallegar, N., K.; Garland, C., J.; Mahendralingam, M.; Viloria-Petit, A., M.; and Christian, S., L.\n\n\n \n\n\n\n Journal of Mammary Gland Biology and Neoplasia, 24(1): 85-97. 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"A$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {A Novel 3-Dimensional Co-culture Method Reveals a Partial Mesenchymal to Epithelial Transition in Breast Cancer Cells Induced by Adipocytes},\n type = {article},\n year = {2019},\n keywords = {3-dimensional culture,Adipocytes,Co-culture system,ECM,Mesenchymal to epithelial transition,Metastasis,Triple negative breast cancer},\n pages = {85-97},\n volume = {24},\n publisher = {Journal of Mammary Gland Biology and Neoplasia},\n id = {6a86ad65-56a2-3295-941b-5b2b60b6f473},\n created = {2020-11-18T19:53:58.394Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:17.378Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Cancer metastases are accountable for almost 90% of all human cancer related deaths including from breast cancer (BC). Adipocytes can alter the tumor microenvironment, which can promote metastasis by inducing an epithelial-to-mesenchymal transition (EMT) in BC cells. However, the role of adipocytes during the mesenchymal-to-epithelial transition (MET), that can be important in metastasis, is not clear. To understand the effect of adipocytes on the BC progression, there is a requirement for a better in vitro 3-dimensional (3D) co-culture system that mimics the breast tissue and allows for more accurate analysis of EMT and MET. We developed a co-culture system to analyze the relationship of BC cells grown in a 3D culture with adipocytes. We found that adipocytes and adipocyte-derived conditioned media, but not pre-adipocytes, caused the mesenchymal MDA-MB-231 and Hs578t cells to form significantly more epithelial-like structures when compared to the typical stellate colonies formed in control 3D cultures. SUM159 cells and MCF7 cells had a less dramatic shift as they normally have more epithelial-like structure in 3D culture. Biomarker expression analysis revealed that adipocytes only induced a partial MET with proliferation unaffected. In addition, adipocytes had reduced lipid droplet size when co-cultured with BC cells. Thus, we found that physical interaction with adipocytes and ECM changes the mesenchymal phenotype of BC cells in a manner that could promote secondary tumor formation.},\n bibtype = {article},\n author = {Pallegar, Nikitha K. and Garland, Chantae J. and Mahendralingam, Mathepan and Viloria-Petit, Alicia M. and Christian, Sherri L.},\n doi = {10.1007/s10911-018-9420-4},\n journal = {Journal of Mammary Gland Biology and Neoplasia},\n number = {1}\n}

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\n \n\n \n \n \n \n \n \n Vibrogen-2 vaccine trial in lumpfish (Cyclopterus lumpus) against Vibrio anguillarum.\n \n \n \n \n\n\n \n Chakraborty, S.; Cao, T.; Hossain, A.; Gnanagobal, H.; Vasquez, I.; Boyce, D.; and Santander, J.\n\n\n \n\n\n\n Journal of Fish Diseases, 42(7): 1057-1064. 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"Vibrogen-2$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Vibrogen-2 vaccine trial in lumpfish (Cyclopterus lumpus) against Vibrio anguillarum},\n type = {article},\n year = {2019},\n keywords = {Vibrio anguillarum,Vibrogen-2,cleaner fish,lumpfish,vaccine},\n pages = {1057-1064},\n volume = {42},\n id = {43ec255b-b14b-3d58-84ad-1995cf38dd11},\n created = {2020-11-18T19:53:58.633Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:01.832Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to biocontrol sea lice infestations in Atlantic salmon aquaculture. However, bacterial infections are affecting cleaner fish performance. Vibrio anguillarum, the aetiological agent of vibriosis, is one of the most frequent bacterial infections in lumpfish, and effective vaccine programmes against this pathogen have been identified as a high priority for lumpfish. Vibrogen-2 is a commercial polyvalent bath vaccine that contains formalin-inactivated cultures of V. anguillarum serotypes O1 and O2, and Vibrio ordalii. In this study, we evaluated Vibrogen-2 efficacy in lumpfish against a local isolated V. anguillarum strain. Two groups of 125 lumpfish were bath-immunized, bath-boost-immunized at four weeks post-primary immunization, and intraperitoneally (i.p.) boost-immunized at eight weeks post-primary immunization. The control groups were i.p. mock-immunized with PBS. Twenty-seven weeks post-primary immunization, the fish were i.p. challenged with 10 or 100 times the V. anguillarum J360 LD50 dose. After the challenge, survival was monitored daily, and samples of tissues were collected at ten days post-challenge. Commercial vaccine Vibrogen-2 reduced V. anguillarum tissue colonization and delayed mortality but did not confer immune protection to C. lumpus against the V. anguillarum i.p. challenge.},\n bibtype = {article},\n author = {Chakraborty, Setu and Cao, Trung and Hossain, Ahmed and Gnanagobal, Hajarooba and Vasquez, Ignacio and Boyce, Danny and Santander, Javier},\n doi = {10.1111/jfd.13010},\n journal = {Journal of Fish Diseases},\n number = {7}\n}

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\n \n\n \n \n \n \n \n \n Aeromonas salmonicida infects Atlantic salmon (Salmo salar) erythrocytes.\n \n \n \n \n\n\n \n Valderrama, K.; Soto-Dávila, M.; Segovia, C.; Vásquez, I.; Dang, M.; and Santander, J.\n\n\n \n\n\n\n Journal of Fish Diseases, 42(11): 1601-1608. 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"Aeromonas$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

\n

@article{\n title = {Aeromonas salmonicida infects Atlantic salmon (Salmo salar) erythrocytes},\n type = {article},\n year = {2019},\n keywords = {Aeromonas salmonicida,Salmo salar,erythrocytes,furunculosis},\n pages = {1601-1608},\n volume = {42},\n id = {44b53b05-0bb8-378c-bd78-211556c61d13},\n created = {2020-11-18T19:53:58.803Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:09.003Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Aeromonas salmonicida subsp. salmonicida (hereafter A. salmonicida) is the aetiological agent of furunculosis in marine and freshwater fish. Once A. salmonicida invade the fish host through skin, gut or gills, it spreads and colonizes the head kidney, liver, spleen and brain. A. salmonicida infects leucocytes and exhibits an extracellular phase in the blood of the host; however, it is unknown whether A. salmonicida have an intraerythrocytic phase. Here, we evaluate whether A. salmonicida infects Atlantic salmon (Salmo salar) erythrocytes in vitro and in vivo. A. salmonicida did not kill primary S. salar erythrocytes, even in the presence of high bacterial loads, but A. salmonicida invaded the S. salar erythrocytes in the absence of evident haemolysis. Naïve Atlantic salmon smolts intraperitoneally infected with A. salmonicida showed bacteraemia 5 days post-infection and the presence of intraerythrocytic A. salmonicida. Our results reveal a novel intraerythrocytic phase during A. salmonicida infection.},\n bibtype = {article},\n author = {Valderrama, Katherinne and Soto-Dávila, Manuel and Segovia, Cristopher and Vásquez, Ignacio and Dang, My and Santander, Javier},\n doi = {10.1111/jfd.13077},\n journal = {Journal of Fish Diseases},\n number = {11}\n}

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\n \n\n \n \n \n \n \n \n Aeromonas salmonicidasubsp. SalmonicidaEarly infection and immune response of atlantic cod (Gadus morhuaL.) primary macrophages.\n \n \n \n \n\n\n \n Soto-Dávila, M.; Hossain, A.; Chakraborty, S.; Rise, M., L.; and Santander, J.\n\n\n \n\n\n\n Frontiers in Immunology, 10(JUN): 1-14. 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"Aeromonas$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

\n

@article{\n title = {Aeromonas salmonicidasubsp. SalmonicidaEarly infection and immune response of atlantic cod (Gadus morhuaL.) primary macrophages},\n type = {article},\n year = {2019},\n keywords = {Aeromonas salmonicida,Atlantic cod,Gadiform,Gram-negative,Innate immunity,Primary macrophages},\n pages = {1-14},\n volume = {10},\n id = {3b6bb2cf-146a-3572-96ac-fddcb584e136},\n created = {2020-11-18T19:53:58.819Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:11.519Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {In contrast to other teleosts, Atlantic cod (Gadus morhua) has an expanded repertoire of MHC-I and TLR components, but lacks the MHC-II, the invariant chain/CD74, and CD4+ T cell response, essential for production of antibodies and prevention of bacterial infectious diseases. The mechanisms by which G. morhua fight bacterial infections are not well understood. Aeromonas salmonicida subsp. salmonicida is a recurrent pathogen in cultured and wild fish, and has been reported in Atlantic cod. Macrophages are some of the first responders to bacterial infection and the link between innate and adaptive immune response. Here, we evaluated the viability, reactive oxygen species (ROS) production, cell morphology, and gene expression of cod primary macrophages in response to A. salmonicida infection. We found that A. salmonicida infects cod primary macrophages without killing the cod cells. Likewise, infected Atlantic cod macrophages up-regulated key genes involved in the inflammatory response (e.g., IL-1β and IL-8) and bacterial recognition (e.g., BPI/LBP). Nevertheless, our results showed a down-regulation of genes related to antimicrobial peptide and ROS production, suggesting that A. salmonicida utilizes its virulence mechanisms to control and prevent macrophage anti-bacterial activity. Our results also indicate that Atlantic cod has a basal ROS production in non-infected cells, and this was not increased after contact with A. salmonicida. Transmission electron microscopy results showed that A. salmonicida was able to infect the macrophages in a high number, and release outer membrane vesicles (OMV) during intracellular infection. These results suggest that Atlantic cod macrophage innate immunity is able to detect A. salmonicida and trigger an anti-inflammatory response, however A. salmonicida controls the cell immune response to prevent bacterial clearance, during early infection.},\n bibtype = {article},\n author = {Soto-Dávila, Manuel and Hossain, Ahmed and Chakraborty, Setu and Rise, Matthew L. and Santander, Javier},\n doi = {10.3389/fimmu.2019.01237},\n journal = {Frontiers in Immunology},\n number = {JUN}\n}

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\n \n\n \n \n \n \n \n \n TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific.\n \n \n \n \n\n\n \n Pitts, M., S.; McShane, J., N.; Hoener, M., C.; Christian, S., L.; and Berry, M., D.\n\n\n \n\n\n\n Histochemistry and Cell Biology, 152(2): 155-166. 2019.\n \n\n\n\n
\n\n\n\n \n \n $\"TAAR1$ Paper\n \n \n \n $\"TAAR1$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

\n

@article{\n title = {TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific},\n type = {article},\n year = {2019},\n keywords = {Bioinformatics,Breast cancer,Confocal microscopy,Flow cytometry,Trace amine-associated receptor 1},\n pages = {155-166},\n volume = {152},\n websites = {https://doi.org/10.1007/s00418-019-01791-7},\n publisher = {Springer Berlin Heidelberg},\n id = {33b91a23-89f8-33f8-84db-b30261addbad},\n created = {2020-11-18T19:53:59.018Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:13.679Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Trace amine-associated receptors are G protein-coupled receptors of which TAAR1 is the most well-studied. Recently, Vattai et al. (J Cancer Res Clin Oncol 143:1637–1647 https://doi.org/10.1007/s00432-017-2420-8, 2017) reported that expression of TAAR1 may be a marker of breast cancer (BC) survival, with a positive correlation also suggested between TAAR1 expression and HER2 positivity. Neither a role for TAAR1 in breast tissue, nor in cancer, had previously been suspected. We, therefore, sought to provide independent validation and to further examine these putative relationships. First, a bioinformatic analysis on 58 total samples including normal breast tissue, BC-related cell lines, and tumour samples representing different BC sub-types found no clear correlation between TAAR1 mRNA levels and any BC subtype, including HER2 +. We next confirmed the bioinformatics data correlated to protein expression using a well validated anti-human TAAR1 antibody. TAAR1 mRNA levels correlated with the relative intensity of immunofluorescence staining in six BC cell lines (MCF-7, T47D, MDA-MB-231, SKBR3, MDA-MB-468, BT-474), but not in the MCF-10A immortalized mammary gland line, which had high mRNA but low protein levels. As expected, TAAR1 protein was intracellular in all cell lines. Surprisingly MCF-7, SKBR3, and MDA-MB-468 showed pronounced nuclear localization. The relative protein expression in MCF-7, MDA-MB-231, and MCF-10A lines was further confirmed by semi-quantitative flow cytometry. Finally, we demonstrate that the commercially available anti-TAAR1 antibody has poor selectivity, which likely explains the lack of correlation with the previous study. Therefore, while we clearly demonstrate variable expression and sub-cellular localization of TAAR1 across BC cell lines, we find no evidence for association with BC subtype.},\n bibtype = {article},\n author = {Pitts, Mallory S. and McShane, Josh N. and Hoener, Marius C. and Christian, Sherri L. and Berry, Mark D.},\n doi = {10.1007/s00418-019-01791-7},\n journal = {Histochemistry and Cell Biology},\n number = {2}\n}

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\n \n 2018\n \n \n (1)\n \n \n

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\n \n \n

\n \n\n \n \n \n \n \n \n Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages.\n \n \n \n \n\n\n \n Eslamloo, K.; Inkpen, S., M.; Rise, M., L.; and Andreassen, R.\n\n\n \n\n\n\n Molecular Immunology, 93(November 2017): 152-161. 2018.\n \n\n\n\n
\n\n\n\n \n \n $\"Discovery$ Paper\n \n \n \n $\"Discovery$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages},\n type = {article},\n year = {2018},\n keywords = {Gadus morhua,Gene expression regulation,Poly(I:C),Small RNA sequencing,Teleost fish,miRNA},\n pages = {152-161},\n volume = {93},\n websites = {https://doi.org/10.1016/j.molimm.2017.11.015},\n publisher = {Elsevier},\n id = {23f67b1c-ac47-3692-9fbd-34771dfc4810},\n created = {2020-11-18T19:53:58.987Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:12.161Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {MicroRNAs (miRNAs) are known to play important immunoregulatory roles in teleosts, although miRNAs involved in the antiviral immune response of Atlantic cod (Gadus morhua) were previously uncharacterised. Using deep sequencing and qPCR, the present study was conducted to identify miRNAs responsive to the viral mimic, polyriboinosinic polyribocytidylic acid (pIC) in Atlantic cod macrophages. Macrophage samples isolated from Atlantic cod (n = 3) and treated with pIC or phosphate buffered saline (PBS control) for 24 and 72 h were used for miRNA profiling. Following deep sequencing, DESeq2 analyses identified four (miR-731-3p, miR-125b-3-3p, miR-150-3p and miR-462-3p) and two (miR-2188-3p and miR-462-3p) significantly differentially expressed miRNAs at 24 and 72 h post-stimulation (HPS), respectively. Sequencing-identified miRNAs were subjected to qPCR validation using a larger number of biological replicates (n = 6) exposed to pIC or PBS over time (i.e. 12, 24, 48 and 72 HPS). As in sequencing, miR-731-3p, miR-462-3p and miR-2188-3p showed significant up-regulation by pIC. The sequencing results were not qPCR-validated for miR-125b-3-3p and miR-150-3p as up- and down-regulated miRNAs at 24 HPS, respectively; however, qPCR results showed significant up-regulation in response to pIC stimulation at later time points (i.e. 48 and/or 72 HPS). We also used qPCR to assess the expression of other miRNAs that were previously shown as immune responsive in other vertebrates. qPCR results at 48 and/or 72 HPS revealed that miR-128-3-5p, miR-214-1-5p and miR-451-3p were induced by pIC, whereas miR-30b-3p and miR-199-1-3p expression were repressed in response to pIC. The present study identified ten pIC-stimulated miRNAs, suggesting them as important in antiviral immune responses of Atlantic cod macrophages. Some pIC-responsive miRNAs identified in this study were predicted to target putative immune-related genes of Atlantic cod (e.g. miR-30b-3p targeting herc4), although the regulatory functions of these miRNAs need to be validated by future studies.},\n bibtype = {article},\n author = {Eslamloo, Khalil and Inkpen, Sabrina M. and Rise, Matthew L. and Andreassen, Rune},\n doi = {10.1016/j.molimm.2017.11.015},\n journal = {Molecular Immunology},\n number = {November 2017}\n}

\n

\n\n\n

\n MicroRNAs (miRNAs) are known to play important immunoregulatory roles in teleosts, although miRNAs involved in the antiviral immune response of Atlantic cod (Gadus morhua) were previously uncharacterised. Using deep sequencing and qPCR, the present study was conducted to identify miRNAs responsive to the viral mimic, polyriboinosinic polyribocytidylic acid (pIC) in Atlantic cod macrophages. Macrophage samples isolated from Atlantic cod (n = 3) and treated with pIC or phosphate buffered saline (PBS control) for 24 and 72 h were used for miRNA profiling. Following deep sequencing, DESeq2 analyses identified four (miR-731-3p, miR-125b-3-3p, miR-150-3p and miR-462-3p) and two (miR-2188-3p and miR-462-3p) significantly differentially expressed miRNAs at 24 and 72 h post-stimulation (HPS), respectively. Sequencing-identified miRNAs were subjected to qPCR validation using a larger number of biological replicates (n = 6) exposed to pIC or PBS over time (i.e. 12, 24, 48 and 72 HPS). As in sequencing, miR-731-3p, miR-462-3p and miR-2188-3p showed significant up-regulation by pIC. The sequencing results were not qPCR-validated for miR-125b-3-3p and miR-150-3p as up- and down-regulated miRNAs at 24 HPS, respectively; however, qPCR results showed significant up-regulation in response to pIC stimulation at later time points (i.e. 48 and/or 72 HPS). We also used qPCR to assess the expression of other miRNAs that were previously shown as immune responsive in other vertebrates. qPCR results at 48 and/or 72 HPS revealed that miR-128-3-5p, miR-214-1-5p and miR-451-3p were induced by pIC, whereas miR-30b-3p and miR-199-1-3p expression were repressed in response to pIC. The present study identified ten pIC-stimulated miRNAs, suggesting them as important in antiviral immune responses of Atlantic cod macrophages. Some pIC-responsive miRNAs identified in this study were predicted to target putative immune-related genes of Atlantic cod (e.g. miR-30b-3p targeting herc4), although the regulatory functions of these miRNAs need to be validated by future studies.\n

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\n \n 2017\n \n \n (1)\n \n \n

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\n \n\n \n \n \n \n \n \n CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo.\n \n \n \n \n\n\n \n Ayre, D., C.; Chute, I., C.; Joy, A., P.; Barnett, D., A.; Hogan, A., M.; Grüll, M., P.; Peña-Castillo, L.; Lang, A., S.; Lewis, S., M.; and Christian, S., L.\n\n\n \n\n\n\n Scientific Reports, 7(1): 1-16. 2017.\n \n\n\n\n
\n\n\n\n \n \n $\"CD24$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@article{\n title = {CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo},\n type = {article},\n year = {2017},\n pages = {1-16},\n volume = {7},\n id = {83dae094-1da3-3ee5-90a3-38577969c78d},\n created = {2020-11-18T19:53:59.004Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:14.537Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma cells in response to CD24 stimulation. We found that B cells constitutively release MVs of approximately 120 nm, and that CD24 induces an increase in phosphatidylserine-positive MV release. RNA cargo is predominantly comprised of 5S rRNA, regardless of stimulation; however, CD24 causes a decrease in the incorporation of protein coding transcripts. The MV proteome is enriched with mitochondrial and metabolism-related proteins after CD24 stimulation; however, these changes were variable and could not be fully validated by Western blotting. CD24-bearing MVs carry Siglec-2, CD63, IgM, and, unexpectedly, Ter119, but not Siglec-G or MHC-II despite their presence on the cell surface. CD24 stimulation also induces changes in CD63 and IgM expression on MVs that is not mirrored by the changes in cell surface expression. Overall, the composition of these MVs suggests that they may be involved in releasing mitochondrial components in response to pro-apoptotic stress with changes to the surface receptors potentially altering the cell type(s) that interact with the MVs.},\n bibtype = {article},\n author = {Ayre, D. Craig and Chute, Ian C. and Joy, Andrew P. and Barnett, David A. and Hogan, Andrew M. and Grüll, Marc P. and Peña-Castillo, Lourdes and Lang, Andrew S. and Lewis, Stephen M. and Christian, Sherri L.},\n doi = {10.1038/s41598-017-08094-8},\n journal = {Scientific Reports},\n number = {1}\n}

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\n \n 2016\n \n \n (3)\n \n \n

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\n \n\n \n \n \n \n \n \n Transcriptome profiling of the antiviral immune response in Atlantic cod macrophages.\n \n \n \n \n\n\n \n Eslamloo, K.; Xue, X.; Booman, M.; Smith, N., C.; and Rise, M., L.\n\n\n \n\n\n\n Developmental & Comparative Immunology. 2016.\n \n\n\n\n
\n\n\n\n \n \n $\"Transcriptome$ Paper\n \n \n \n $\"Transcriptome$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@article{\n title = {Transcriptome profiling of the antiviral immune response in Atlantic cod macrophages},\n type = {article},\n year = {2016},\n websites = {http://linkinghub.elsevier.com/retrieve/pii/S0145305X16301744},\n publisher = {Elsevier Ltd},\n id = {8756849d-10a6-3873-b405-babeb09a16b5},\n created = {2020-11-18T19:53:58.809Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:08.275Z},\n read = {true},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n bibtype = {article},\n author = {Eslamloo, Khalil and Xue, Xi and Booman, Marije and Smith, Nicole C. and Rise, Matthew L.},\n doi = {10.1016/j.dci.2016.05.021},\n journal = {Developmental & Comparative Immunology}\n}

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\n \n\n \n \n \n \n \n \n The biodegradation of crude oil in the deep ocean.\n \n \n \n \n\n\n \n Prince, R., C.; Nash, G., W.; and Hill, S., J.\n\n\n \n\n\n\n Marine Pollution Bulletin. 7 2016.\n \n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n \n $\"The$ Website\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@article{\n title = {The biodegradation of crude oil in the deep ocean},\n type = {article},\n year = {2016},\n websites = {http://linkinghub.elsevier.com/retrieve/pii/S0025326X1630491X},\n month = {7},\n id = {861ff6e8-84c6-3477-aa06-e4016ef5459f},\n created = {2020-11-18T19:53:58.834Z},\n accessed = {2016-07-13},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:10.540Z},\n read = {true},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Oil biodegradation at a simulated depth of 1500m was studied in a high-pressure apparatus at 5°C, using natural seawater with its indigenous microbes, and 3ppm of an oil with dispersant added at a dispersant:oil ratio of 1:15. Biodegradation of the detectable hydrocarbons was prompt and extensive (>70% in 35days), although slower by about a third than under otherwise identical conditions equivalent to the surface. The apparent half-life of biodegradation of the total detectable hydrocarbons at 15MPa was 16days (compared to 13days at atmospheric pressure), although some compounds, such as the four-ring aromatic chrysene, were degraded rather more slowly.},\n bibtype = {article},\n author = {Prince, Roger C. and Nash, Gordon W. and Hill, Stephen J.},\n doi = {10.1016/j.marpolbul.2016.06.087},\n journal = {Marine Pollution Bulletin}\n}

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\n \n\n \n \n \n \n \n \n The external morphology of adult female Egrasilus labracis as shown using hexamethyldisilazane treated, uncoated specimens for scanning electron microscopy.\n \n \n \n \n\n\n \n Murray, H., M.; Hill, S., J.; and Ang, K., P.\n\n\n \n\n\n\n Microscopy Research and Technique, 00: 1-7. 2016.\n \n\n\n\n
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@article{\n title = {The external morphology of adult female <i>Egrasilus labracis</i> as shown using hexamethyldisilazane treated, uncoated specimens for scanning electron microscopy},\n type = {article},\n year = {2016},\n keywords = {antennae,hmds,mandible,parasitic copepod,surface striation,swimming legs},\n pages = {1-7},\n volume = {00},\n websites = {http://doi.wiley.com/10.1002/jemt.22680},\n id = {16996ce1-1c7d-344f-aa0f-cd7d4d1ebbea},\n created = {2020-11-18T19:53:59.137Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:16.021Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n bibtype = {article},\n author = {Murray, Harry M. and Hill, Stephen J. and Ang, Keng P.},\n doi = {10.1002/jemt.22680},\n journal = {Microscopy Research and Technique}\n}

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\n \n\n \n \n \n \n \n \n Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.\n \n \n \n \n\n\n \n Ayre, D., C.; Elstner, M.; Smith, N., C.; Moores, E., S.; Hogan, A., M.; and Christian, S., L.\n\n\n \n\n\n\n Immunology, 146(2): 217-233. 2015.\n \n\n\n\n
\n\n\n\n \n \n $\"Dynamic$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n

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@article{\n title = {Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement},\n type = {article},\n year = {2015},\n keywords = {Apoptosis,B lymphocyte,Bioinformatics,CD24,Microvesicle},\n pages = {217-233},\n volume = {146},\n id = {71b71550-8d61-398c-9ac0-728bef102824},\n created = {2020-11-18T19:53:59.051Z},\n file_attached = {true},\n profile_id = {a61953dd-7169-39f2-8a6e-a6909b400142},\n group_id = {966e73af-1c90-35b5-82ca-7b5301de37a2},\n last_modified = {2020-11-18T19:54:15.390Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {The glycophosphatidylinositol-anchored cell surface receptor CD24 (also called heat-stable antigen) promotes the apoptosis of progenitor and precursor B-lymphocytes. However, the immediate proximal events that occur after engagement of CD24 in B cells are not precisely understood. Using a bioinformatics analysis of mouse (Mus musculus) gene expression data from the Immunological Genome Project, we found that known vesicle trafficking and cellular organization genes have similar expression patterns to CD24 during B-cell development in the bone marrow. We therefore hypothesized that CD24 regulates vesicle trafficking. We first validated that antibody-mediated engagement of CD24 induces apoptosis in the mouse WEHI-231 cell line and mouse primary bone marrow-derived B cells. We next found that CD24 surface protein expression is rapidly and dynamically regulated in both WEHI-231 cells and primary immature B cells in response to engagement of CD24. The change in surface expression was not mediated by classical endocytosis or exocytosis. However, we found that CD24-bearing plasma membrane-derived extracellular microvesicles were released in response to CD24 engagement. Furthermore, in response to CD24 engagement we observed a clear exchange of CD24 between different populations of B cells. Hence, we show that engagement of CD24 in immature B cells results in a dynamic regulation of surface CD24 protein and a redistribution of CD24 within the population.},\n bibtype = {article},\n author = {Ayre, D. Craig and Elstner, Marcus and Smith, Nicole C. and Moores, Emily S. and Hogan, Andrew M. and Christian, Sherri L.},\n doi = {10.1111/imm.12493},\n journal = {Immunology},\n number = {2}\n}

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