, 8(1): 26-38. 2017.\n
\n
@article{\n title = {Data Analysis of Multiplex Sequencing at SOLiD Platform: A Probabilistic Approach to Characterization and Reliability Increase},\n type = {article},\n year = {2017},\n keywords = {Health Informatics,Probabilistic Modeling,SOLiD},\n pages = {26-38},\n volume = {8},\n websites = {http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajmb.2018.81003},\n publisher = {Scientific Research Publishing},\n id = {e31cb219-c970-3315-a319-efd04ce31b2b},\n created = {2020-04-15T19:49:22.640Z},\n file_attached = {false},\n profile_id = {bd1f7f2e-7152-3c06-841d-eee1e29b03d2},\n group_id = {0ea50baf-be5a-3ec5-9548-2232bf737587},\n last_modified = {2023-04-14T18:04:12.574Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {false},\n hidden = {false},\n citation_key = {Lobato2017},\n source_type = {article},\n private_publication = {false},\n abstract = {New sequencing technologies such as Illumina/Solexa, SOLiD/ABI, and 454/Roche, revolutionized the biological researches. In this context, the SOLiD platform has a particular sequencing type, known as multiplex run, which enables the sequencing of several samples in a single run. It implies in cost reduction and simplifies the analysis of related samples. Meanwhile, this sequencing type requires an additional filtering step to ensure the reliability of the results. Thus, we propose in this paper a probabilistic model which considers the intrinsic characteristics of each sequencing to characterize multiplex runs and filter low-quality data, increasing the data analysis reliability of multiplex sequencing performed on SOLiD. The results show that the proposed model proves to be satisfactory due to: 1) identification of faults in the sequencing process; 2) adaptation and development of new protocols for sample preparation; 3) the assignment of a degree of confidence to the data generated; and 4) guiding a filtering process, without discarding useful sequences in an arbitrary manner.},\n bibtype = {article},\n author = {Lobato, Fábio Manoel França and Damasceno, Carlos Diego and Leite, Daniela Soares and Ribeiro-dos-Santos, Ândrea Kelly and Darnet, Sylvain and Francês, Carlos Renato and Vijaykumar, Nandamudi Lankalapalli and de Santana, Ádamo Lima and França Lobato, Fábio Manoel and Damasceno, Carlos Diego and Soares Leite, Daniela and Ribeiro-dos-Santos, Ândrea Kelly and Darnet, Sylvain and Francês, Carlos Renato and Lankalapalli Vijaykumar, Nandamudi and de Santana, Ádamo Lima},\n doi = {10.4236/ajmb.2018.81003},\n journal = {American Journal of Molecular Biology},\n number = {1}\n}
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\n New sequencing technologies such as Illumina/Solexa, SOLiD/ABI, and 454/Roche, revolutionized the biological researches. In this context, the SOLiD platform has a particular sequencing type, known as multiplex run, which enables the sequencing of several samples in a single run. It implies in cost reduction and simplifies the analysis of related samples. Meanwhile, this sequencing type requires an additional filtering step to ensure the reliability of the results. Thus, we propose in this paper a probabilistic model which considers the intrinsic characteristics of each sequencing to characterize multiplex runs and filter low-quality data, increasing the data analysis reliability of multiplex sequencing performed on SOLiD. The results show that the proposed model proves to be satisfactory due to: 1) identification of faults in the sequencing process; 2) adaptation and development of new protocols for sample preparation; 3) the assignment of a degree of confidence to the data generated; and 4) guiding a filtering process, without discarding useful sequences in an arbitrary manner.\n