, 21(8): 2222–2234. nov 2012.\n
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@article{Bouetard2012,\nabstract = {Due to their ability to explore whole genome response to drugs and stressors, omics-based approaches are widely used in toxicology and ecotoxicology, and identified as powerful tools for future ecological risk assessment and environmental monitoring programs. Understanding the long-term effects of contaminants may indeed benefit from the coupling of genomics and eco-evolutionary hypotheses. Next-generation sequencing provides a new way to investigate pollutants impact, by targeting early responses, screening chemicals, and directly quantifying gene expression, even in organisms without reference genome. Lymnaea stagnalis is a freshwater mollusk in which access to genomic resources is critical for many scientific issues, especially in ecotoxicology. We used 454-pyrosequencing to obtain new transcriptomic resources in L. stagnalis and to preliminarily explore gene expression response to a redox-cycling pesticide, diquat. We obtained 151,967 and 128,945 high-quality reads from control and diquat-exposed individuals, respectively. Sequence assembly provided 141,999 contigs, of which 124,387 were singletons. BlastX search revealed significant match for 34.6 {\\%}of the contigs (21.2 {\\%}protein hits).KEGG annotation showed a predominance of hits with genes involved in energy metabolism and circulatory system, and revealed more than 400 putative genes involved in oxidative stress, cellular/molecular stress and signaling pathways, apoptosis, and metabolism of xenobiotics. Results also suggest that diquat may have a great diversity of molecular effects. Moreover, new genetic markers (putative SNPs) were discovered. We also created a Ensembl-like web-tool for data-mining (http://genotoul-contigbrowser.toulouse. inra.fr:9095/Lymnaea stagnalis/index.html). This resource is expected to be relevant for any genomic approach aimed at understanding the molecular basis of physiological and evolutionary responses to environmental stress in L. stagnalis. {\\textcopyright} Springer Science+Business Media, LLC 2012.},\nauthor = {Bou{\\'{e}}tard, Anthony and Noirot, C{\\'{e}}line and Besnard, Anne Laure and Bouchez, Olivier and Choisne, Damien and Robe, Eugenie and Klopp, Christophe and Lagadic, Laurent and Coutellec, Marie Agnes},\ndoi = {10.1007/s10646-012-0977-1},\nissn = {09639292},\njournal = {Ecotoxicology},\nkeywords = {Ecotoxicology,Lymnaea stagnalis,Oxidative stress,Pesticides,Pyrosequencing,Transcriptomics},\nmonth = {nov},\nnumber = {8},\npages = {2222--2234},\npmid = {22814884},\ntitle = {{Pyrosequencing-based transcriptomic resources in the pond snail Lymnaea stagnalis, with a focus on genes involved in molecular response to diquat-induced stress}},\nurl = {http://link.springer.com/10.1007/s10646-012-0977-1},\nvolume = {21},\nyear = {2012}\n}\n
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\n Due to their ability to explore whole genome response to drugs and stressors, omics-based approaches are widely used in toxicology and ecotoxicology, and identified as powerful tools for future ecological risk assessment and environmental monitoring programs. Understanding the long-term effects of contaminants may indeed benefit from the coupling of genomics and eco-evolutionary hypotheses. Next-generation sequencing provides a new way to investigate pollutants impact, by targeting early responses, screening chemicals, and directly quantifying gene expression, even in organisms without reference genome. Lymnaea stagnalis is a freshwater mollusk in which access to genomic resources is critical for many scientific issues, especially in ecotoxicology. We used 454-pyrosequencing to obtain new transcriptomic resources in L. stagnalis and to preliminarily explore gene expression response to a redox-cycling pesticide, diquat. We obtained 151,967 and 128,945 high-quality reads from control and diquat-exposed individuals, respectively. Sequence assembly provided 141,999 contigs, of which 124,387 were singletons. BlastX search revealed significant match for 34.6 %of the contigs (21.2 %protein hits).KEGG annotation showed a predominance of hits with genes involved in energy metabolism and circulatory system, and revealed more than 400 putative genes involved in oxidative stress, cellular/molecular stress and signaling pathways, apoptosis, and metabolism of xenobiotics. Results also suggest that diquat may have a great diversity of molecular effects. Moreover, new genetic markers (putative SNPs) were discovered. We also created a Ensembl-like web-tool for data-mining (http://genotoul-contigbrowser.toulouse. inra.fr:9095/Lymnaea stagnalis/index.html). This resource is expected to be relevant for any genomic approach aimed at understanding the molecular basis of physiological and evolutionary responses to environmental stress in L. stagnalis. © Springer Science+Business Media, LLC 2012.\n