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@article{domenech_studies_2018, title = {Studies in an {Early} {Development} {Window} {Unveils} a {Severe} {HSC} {Defect} in both {Murine} and {Human} {Fanconi} {Anemia}}, volume = {11}, issn = {2213-6711}, doi = {10.1016/j.stemcr.2018.10.001}, abstract = {Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the highest level of hematopoietic stem cells [HSC] amplification takes place) and E14.5. This study reveals a deep HSC defect with exhaustion of proliferative and self-renewal capacities very early during development, together with severe FA clinical and biological manifestations, which are mitigated at E14.5 due to compensatory mechanisms that help to ensure survival of Fancg-/- embryos. It also reports that a deep HSC defect is also observed during human FA development, and that human FA fetal liver (FL) HSCs present a transcriptome profile similar to that of mouse E12.5 Fancg-/- FL HSCs. Altogether, our results highlight that early mouse FL could represent a good alternative model for studying Fanconi pathology.}, language = {eng}, number = {5}, journal = {Stem Cell Reports}, author = {Domenech, Carine and Maillard, Loïc and Rousseau, Alix and Guidez, Fabien and Petit, Laurence and Pla, Marika and Clay, Denis and Guimiot, Fabien and Sanfilippo, Sandra and Jacques, Sebastien and de la Grange, Pierre and Robil, Noémie and Soulier, Jean and Souyri, Michèle}, month = nov, year = {2018}, pmid = {30449320}, pmcid = {PMC6234961}, keywords = {Fanconi anemia, HSC, fetal liver, human embryonic development, mouse embryonic development, placenta, transcriptome}, pages = {1075--1091}, }
@article{desterke_bioinformatics_2018, title = {A bioinformatics transcriptome meta-analysis highlights the importance of trophoblast differentiation in the pathology of hydatidiform moles}, volume = {65}, issn = {1532-3102}, doi = {10.1016/j.placenta.2018.04.002}, abstract = {INTRODUCTION: Hydatidiform mole (HM) is an aberrant human pregnancy with abnormal trophoblastic development, migration/invasion of the extravillous trophoblast in the decidua. These abnormalities are established in a hypoxic environment during the first trimester of gestation. METHODS: Using text mining, we identified 72 unique genes that are linked to HM (HM-linked genes) that we studied by bioinformatic analysis in publicly available transcriptomes of primary chorionic villous cells (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast, and arterial and venous endothelial) isolated from normal placentas or established trophoblastic cell lines cultured under different oxygen concentrations. RESULTS: We show that the majority of HM-linked genes (75\%) are involved in normal trophoblastic differentiation, arranged in clusters, and some of them are implicated in chorionic villous invasion or regulated by oxygen concentrations. DISCUSSION: Our analysis integrates the various aspects of the pathophysiology of HM and highlights the importance of trophoblastic differentiation in this pathology.}, language = {eng}, journal = {Placenta}, author = {Desterke, Christophe and Slim, Rima and Candelier, Jean-Jacques}, month = may, year = {2018}, pmid = {29908639}, pages = {29--36}, }
@article{gentil_aryl_2018, title = {Aryl hydrocarbon receptor ({AHR}) is a novel druggable pathway controlling malignant progenitor proliferation in chronic myeloid leukemia ({CML})}, volume = {13}, issn = {1932-6203}, doi = {10.1371/journal.pone.0200923}, abstract = {Aryl Hydrocarbon Receptor (AHR) is an ubiquitous basic helix-loop-helix transcription factor, which is ligand-activated and involved in numerous biological processes including cell division, cell quiescence and inflammation. It has been shown that AHR is involved in normal hematopoietic progenitor proliferation in human cells. In addition, loss of AHR in knockout mice is accompanied by a myeloproliferative syndrome-like disease, suggesting a role of AHR in hematopoietic stem cell (HSC) maintenance. To study the potential role of AHR pathway in CML progenitors and stem cells, we have first evaluated the expression of AHR in UT-7 cell line expressing BCR-ABL. AHR expression was highly reduced in UT-7 cell expressing BCR-ABL as compared to controls. AHR transcript levels, quantified in primary peripheral blood CML cells at diagnosis (n = 31 patients) were found to be significantly reduced compared to healthy controls (n = 15). The use of StemRegenin (SR1), an AHR antagonist, induced a marked expansion of total leukemic cells and leukemic CD34+ cells by about 4- and 10-fold respectively. SR1-treated CML CD34+ cells generated more colony-forming cells and long-term culture initiating cell (LTC-IC)-derived progenitors as compared to non-SR1-treated counterparts. Conversely, treatment of CML CD34+ cells with FICZ, a natural agonist of AHR, induced a 3-fold decrease in the number of CD34+ cells in culture after 7 days. Moreover, a 4-day FICZ treatment was sufficient to significantly reduce the clonogenic potential of CML CD34+ cells and this effect was synergized by Imatinib and Dasatinib treatments. Similarly, a 3-day FICZ treatment contributed to hinder significantly the number of LTC-IC-derived progenitors without synergistic effect with Imatinib. The analysis of molecular circuitry of AHR signaling in CML showed a transcriptional signature in CML derived CD34+ CD38- primitive cells with either low or high levels of AHR, with an upregulation of myeloid genes involved in differentiation in the "AHR low" fraction and an upregulation of genes involved in stem cell maintenance in the "AHR high" fraction. In conclusion, these findings demonstrate for the first time that down-regulation of AHR expression, a major cell cycle regulator, is involved in the myeloproliferative phenotype associated with CML. AHR agonists inhibit clonogenic and LTC-IC-derived progenitor growth and they could be used in leukemic stem cell targeting in CML.}, language = {eng}, number = {8}, journal = {PloS One}, author = {Gentil, Melanie and Hugues, Patricia and Desterke, Christophe and Telliam, Gladys and Sloma, Ivan and Souza, Lucas E. B. and Baykal, Seda and Artus, Jerome and Griscelli, Frank and Guerci, Agnes and Johnson-Ansah, Hyacinthe and Foudi, Adlen and Bennaceur-Griscelli, Annelise and Turhan, Ali G.}, year = {2018}, pmid = {30091999}, pmcid = {PMC6084853}, pages = {e0200923}, }
@article{lopez_glycocalyx_2018, title = {Glycocalyx {Preservation} and {NO} {Production} in {Fatty} {Livers}-{The} {Protective} {Role} of {High} {Molecular} {Polyethylene} {Glycol} in {Cold} {Ischemia} {Injury}}, volume = {19}, issn = {1422-0067}, doi = {10.3390/ijms19082375}, abstract = {Improving the protection of marginal liver grafts during static cold storage is a major hurdle to increase the donor pool of organs. The endothelium glycocalyx quality of preservation influences future inflammatory and oxidative responses. One cellular pathway responsible for the formation of nitric oxide by endothelial cells is dependent on the stimulation of proteoglycans present in the glycocalyx. We investigated the impact of the glycocalyx preservation in static cold storage of fatty liver preserved in different preservation solutions on the endothelium-mediated production of NO. Zucker fatty rat livers were preserved 24 h in static cold storage in either Institut Georges Lopez-1 (IGL-1) (n = 10), IGL-0 (i.e., without PEG35) (n = 5) or Histidine-Tryptophan-Ketoglutarate (HTK) (n = 10) preservation solutions before being processed for analysis. For Sham group (n = 5), the fatty livers were immediately analyzed after procurement. The level of transaminases and nitrites/nitrates were measured in the washing perfusate. Glycocalyx proteins expressions, Syndecan-1, glypican-1 and heparan sulfate (HS), were determined in the tissue (ELISA). Steatotic livers preserved 24 h in IGL-1 preservation solution have a significant lower level of transaminases (aspartate aminotransferase (AST), alanine aminotransferase (ALT)) and less histological damages than steatotic livers preserved 24 h with HTK (p = 0.0152). The syndecan-1 is significantly better preserved in IGL-1 group compared to HTK (p {\textless} 0.0001) and we observed the same tendency compared to IGL-0. No significant differences were observed with glypican-1. HS expression in HTK group was significantly higher compared to the three other groups. HS level in IGL-1 was even lower than IGL-0 (p = 0.0005) which was similar to Sham group. The better protection of the glycocalyx proteins in IGL-1 group was correlated with a higher production of NO than HTK (p = 0.0055) or IGL-0 (p = 0.0433). IGL-1 protective mechanisms through the formation of NO could be due to its better protective effects on the glycocalyx during SCS compared to other preservation solutions. This beneficial effect could involve the preservation state of syndecan-1 and the internalization of HS.}, language = {eng}, number = {8}, journal = {International Journal of Molecular Sciences}, author = {Lopez, Alexandre and Panisello-Rosello, Arnau and Castro-Benitez, Carlos and Adam, René}, month = aug, year = {2018}, pmid = {30103565}, pmcid = {PMC6121886}, keywords = {cold storage, glycocalyx, ischemia, liver, polyethylene glycol, steatosis}, }
@article{khosravi_differentiation_2018, title = {Differentiation of umbilical cord derived mesenchymal stem cells to hepatocyte cells by transfection of {miR}-106a, {miR}-574-3p, and {miR}-451}, volume = {667}, issn = {1879-0038}, doi = {10.1016/j.gene.2018.05.028}, abstract = {Studying the profile of micro RNAs (miRs) elucidated the highest expressed miRs in hepatic differentiation. In this study, we investigated to clarify the role of three embryonic overexpressed miRs (miR-106a, miR-574-3p and miR-451) during hepatic differentiation of human umbilical cord derived mesenchymal stem cells (UC-MSCs). We furthermore, aimed to explore whether overexpression of any of these miRs alone is sufficient to induce the differentiation of the UC-MSCs into hepatocyte-like cells. UC-MSCs were transfected either alone or together with miR-106a, miR-574-3p and miR-451 and their potential hepatic differentiation and alteration in gene expression profile, morphological changes and albumin secretion ability were investigated. We found that up-regulation of any of these three miRs alone cannot induce expression of all hepatic specific genes. Transfection of each miR alone, led to Sox17, FoxA2 expression that are related to initiation step of hepatic differentiation. However, concurrent ectopic overexpression of three miRs together can induce UC-MSCs differentiation into functionally mature hepatocytes. These results show that miRs have the capability to directly convert UC-MSCs to a hepatocyte phenotype in vitro.}, language = {eng}, journal = {Gene}, author = {Khosravi, Maryam and Azarpira, Negar and Shamdani, Sara and Hojjat-Assari, Suzzan and Naserian, Sina and Karimi, Mohammad Hossein}, month = aug, year = {2018}, pmid = {29763649}, keywords = {Cell Differentiation, Cells, Cultured, Gene Expression Profiling, Gene Regulatory Networks, Hepatocyte Nuclear Factor 3-beta, Hepatocyte differentiation, Hepatocytes, Humans, Mesenchymal Stromal Cells, Micro RNA, MicroRNAs, SOXF Transcription Factors, Tissue engineering, Transfection, Umbilical Cord, Umbilical cord derived mesenchymal stem cells}, pages = {1--9}, }
@article{magne_mesenchymal_2018, title = {Mesenchymal {Stromal} {Cell} {Preconditioning}: {The} {Next} {Step} {Toward} a {Customized} {Treatment} {For} {Severe} {Burn}}, issn = {1557-8534}, shorttitle = {Mesenchymal {Stromal} {Cell} {Preconditioning}}, doi = {10.1089/scd.2018.0094}, abstract = {Over the last century, the clinical management of severe skin burns significantly progressed with the development of burn care units, topical antimicrobials, resuscitation methods, early eschar excision surgeries, and skin grafts. Despite these considerable advances, the present treatment of severe burns remains burdensome, and patients are highly susceptible to skin engraftment failure, infections, organ dysfunction, and hypertrophic scarring. Recent researches have focused on mesenchymal stromal cell (MSC) therapy and hold great promises for tissue repair, as reported in several animal studies and clinical cases. In the present review, we will provide an up-to-date outlook of the pathophysiology of severe skin burns, clinical treatment modalities and current limitations. We will then focus on MSCs and their potential in the burn wound healing both in in vitro and in vivo studies. A specific attention will be paid to the cell preconditioning approach, as a means of improving the MSC efficacy in the treatment of major skin burns. In particular, we will debate how several preconditioning cues would modulate the MSC properties to better match up with the burn pathophysiology in the course of the cell therapy. Finally, we will discuss the clinical interest and feasibility of a MSC-based therapy in comparison to their paracrine derivatives, including microvesicles and conditioned media for the treatment of major skin burn injuries.}, language = {eng}, journal = {Stem Cells and Development}, author = {Magne, Brice and Lataillade, Jean-Jacques and Trouillas, Marina}, month = jul, year = {2018}, pmid = {30039742}, keywords = {cell preconditioning, clinical use, major skin burn injuries and pathophysiology, mesenchymal stromal cells}, }
@article{khosravi_induction_2018, title = {Induction of {CD4}+{CD25}+{Foxp3}+ regulatory {T} cells by mesenchymal stem cells is associated with {RUNX} complex factors}, volume = {66}, issn = {1559-0755}, doi = {10.1007/s12026-017-8973-4}, abstract = {Among the particular immunomodulation properties of mesenchymal stem cells (MSCs), one relies on their capacity to regulatory T cell (Treg) induction from effector T cells. Stable expression of Foxp3 has a dominant role in suppressive phenotype and stability of induced regulatory T cells (iTregs). How MSCs induce stable Foxp3 expression in iTregs remains unknown. We previously showed MSCs could enhance demethylation of Treg-specific demethylated region (TSDR) in iTregs in cell-cell contact manner (unpublished data). Here, we evaluated the possible effect of MSCs on the mRNA expression of Runx complex genes (Runx1, Runx3, and CBFB) that perch on TSDR in iTregs and play the main role in suppressive properties of Tregs, a regulatory pathway that has not yet been explored by MSCs. Also, we investigated the mRNA expression of MBD2 that promotes TSDR demethylation in Tregs. We first showed that in vitro MSC-iTreg induction was associated with strong mRNA modifications of genes involved in Runx complex. We next injected high doses of MSCs in a murine model of C57BL/6 into Balb/C allogeneic skin transplantation to prolong allograft survival. When splenocytes of grafted mice were analyzed, we realized that the Foxp3 expression was increased at day 5 and 10 post-graft merely in MSC-treated mice. Furthermore, Foxp3 mRNA expression was associated with modified Runx complex mRNA expression comparable to what was shown in in vitro studies. Hence, our data identify a possible mechanism in which MSCs convert conventional T cells to iTreg through strong modifications of mRNA of genes that are involved in Runx complex of Foxp3.}, language = {eng}, number = {1}, journal = {Immunologic Research}, author = {Khosravi, Maryam and Bidmeshkipour, Ali and Moravej, Ali and Hojjat-Assari, Suzzan and Naserian, Sina and Karimi, Mohammad Hossein}, month = feb, year = {2018}, pmid = {29143918}, keywords = {Demethylation, Mesenchymal stem cells, Regulatory T cells, Runx complex}, pages = {207--218}, }
@article{girerd_superoxide_2018, title = {Superoxide dismutase 2 ({SOD2}) contributes to genetic stability of native and {T315I}-mutated {BCR}-{ABL} expressing leukemic cells}, volume = {498}, issn = {1090-2104}, doi = {10.1016/j.bbrc.2018.03.023}, abstract = {Manganese Superoxide dismutase 2 (SOD2) plays a crucial role in antioxidant defense but there are no data suggesting its role in genetic instability in CML. We evaluated the effects of SOD2 silencing in human UT7 cell line expressing either non-mutated or T315I-mutated BCR-ABL. Array-CGH experiments detected in BCR-ABL-expressing cells silenced for SOD2 a major genetic instability within several chromosomal loci, especially in regions carrying the glypican family (duplicated) and β-defensin genes (deleted). In a large cohort of patients with chronic myeloid leukemia (CML), a significant decrease of SOD2 mRNA was observed. This reduction appeared inversely correlated with leukocytosis and Sokal score, high-risk patients showing lower SOD2 levels. The analysis of anti-oxidant gene expression analysis revealed a specific down-regulation of the expression of PRDX2 in UT7-BCR-ABL and UT7-T315I cells silenced for SOD2 expression. Gene set enrichment analysis performed between the two SOD2-dependent classes of CML patients revealed a significant enrichment of Reactive Oxygen Species (ROS) Pathway. Our data provide the first evidence for a link between SOD2 expression and genetic instability in CML. Consequently, SOD2 mRNA levels should be analyzed in prospective studies as patients with low SOD2 expression could be more prone to develop a mutator phenotype under TKI therapies.}, language = {eng}, number = {4}, journal = {Biochemical and Biophysical Research Communications}, author = {Girerd, Sandrine and Tosca, Lucie and Herault, Olivier and Vignon, Christine and Biard, Denis and Aggoune, Djamel and Dkhissi, Fatima and Bonnet, Marie Laure and Sorel, Nathalie and Desterke, Christophe and Bennaceur-Griscelli, Annelise and Tachdjian, Gerard and Guilhot, François and Guilhot, Joelle and Chomel, Jean-Claude and Turhan, Ali G.}, year = {2018}, pmid = {29550484}, keywords = {CML, Cell Line, Tumor, Cohort Studies, Fusion Proteins, bcr-abl, Gene Expression Regulation, Leukemic, Gene Silencing, Genetic instability, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Mutation, Peroxiredoxins, Point Mutation, SOD2, Silencing, Superoxide Dismutase}, pages = {715--722}, }
@article{desterke_experimental_2018, title = {Experimental and integrative analyses identify an {ETS1} network downstream of {BCR}-{ABL} in chronic myeloid leukemia ({CML})}, issn = {1873-2399}, doi = {10.1016/j.exphem.2018.04.007}, abstract = {The BCR-ABL oncogene, the hallmark of chronic myeloid leukemia (CML), has been shown to activate several signaling pathways in leukemic cells. The natural history of this disease has been radically modified by tyrosine kinase inhibitors (TKIs). However, resistance to several lines of TKI therapies and progression to blast crisis (BC) remain significant concerns. To identify novel signaling pathways induced by BCR-ABL, we performed a transcriptome analysis in a BCR-ABL-expressing UT-7 cell line. More than 2000 genes differentially expressed between BCR-ABL-expressing and parental UT-7 cells were identified and ETS1 was found to be the most upregulated. ETS1 protein expression was also shown to be highly increased in UT-7 cells expressing BCR-ABL either constitutively or under the control of TET-inducible promoters. ETS1 expression is tyrosine-kinase dependent because it was reduced by TKIs. A significant increase of ETS1 messenger RNA (mRNA) expression was observed in blood cells from CML patients at diagnosis compared with healthy controls. Integration of publicly available chromatin immunoprecipitation sequencing and transcriptomic data with our results allowed us to identify potential ETS1 targets, some of which are involved in the progression of CML. The messenger RNA expression of two of these genes (DNM3 and LIMS1) was found to be associated with the absence of major cytogenetic response after 1 year of imatinib therapy. The present work demonstrates for the first time the involvement of the ETS1 transcriptional program in the experimental UT-7 model and a large cohort of CML patients.}, language = {eng}, journal = {Experimental Hematology}, author = {Desterke, Christophe and Voldoire, Maud and Bonnet, Marie-Laure and Sorel, Nathalie and Pagliaro, Sarah and Rahban, Hind and Bennaceur-Griscelli, Annelise and Cayssials, Emilie and Chomel, Jean-Claude and Turhan, Ali G.}, month = may, year = {2018}, pmid = {29733872}, }
@article{darnaud_enteric_2018, title = {Enteric {Delivery} of {Regenerating} {Family} {Member} 3 alpha {Alters} the {Intestinal} {Microbiota} and {Controls} {Inflammation} in {Mice} {With} {Colitis}}, volume = {154}, issn = {1528-0012}, doi = {10.1053/j.gastro.2017.11.003}, abstract = {BACKGROUND \& AIMS: Paneth cell dysfunction causes deficiencies in intestinal C-type lectins and antimicrobial peptides, which leads to dysbiosis of the intestinal microbiota, alters the mucosal barrier, and promotes development of inflammatory bowel diseases. We investigated whether transgenic (TG) expression of the human regenerating family member 3 alpha gene (REG3A) alters the fecal microbiota and affects development of colitis in mice. METHODS: We performed studies with C57BL/6 mice that express human regenerating family member 3 alpha (hREG3A) in hepatocytes, via the albumin gene promoter. In these mice, hREG3A travels via the bile to the intestinal lumen. Some mice were given dextran sodium sulfate (DSS) to induce colitis. Feces were collected from mice and the composition of the microbiota was analyzed by 16S ribosomal RNA sequencing. The fecal microbiome was also analyzed from mice that express only 1 copy of human REG3A transgene but were fed feces from control mice (not expressing hREG3A) as newborns. Mice expressing hREG3A were monitored for DSS-induced colitis after cohousing or feeding feces from control mice. Colitis was induced in another set of control and hREG3A-TG mice by administration of trinitrobenzene sulfonic acid; some mice were given intrarectal injections of the hREG3A protein. Colon tissues were collected from mice and analyzed by histology and immunohistochemistry to detect mucin 2, as well as by 16S ribosomal RNA fluorescence in situ hybridization, transcriptional analyses, and quantitative polymerase chain reaction. We measured levels of reactive oxygen species (ROS) in bacterial cultures and fecal microbiota using 2',7'-dichlorofluorescein diacetate and flow cytometry. RESULTS: The fecal microbiota of mice that express hREG3A had a significant shift in composition, compared with control mice, with enrichment of Clostridiales (Ruminococcaceae, Lachnospiraceae) and depletion of Bacteroidetes (Prevotellaceae); the TG mice developed less-severe colitis following administration of DSS than control mice, associated with preserved gut barrier integrity and reduced bacterial translocation, epithelial inflammation, and oxidative damage. A similar shift in the composition of the fecal microbiota occurred after a few months in TG mice heterozygous for REG3A that harbored a wild-type maternal microbiota at birth; these mice developed less-severe forms of colitis following DSS administration. Cohoused and germ-free mice fed feces from REG3A-TG mice and given DSS developed less-severe forms of colitis and had reduced lipopolysaccharide activation of the toll-like receptor 4 and increased survival times compared with mice not fed feces from REG3A-TG mice. REG3A TG mice developed only mild colonic inflammation after exposure to 2,4,6-trinitrobenzene sulfonic acid, compared with control mice. Control mice given intrarectal hREG3A and exposed to 2,4,6-trinitrobenzene sulfonic acid showed less colon damage and inflammation than mice not given intrarectal hREG3A. Fecal samples from REG3A-TG mice had lower levels of ROS than feces from control mice during DSS administration. Addition of hREG3A to bacterial cultures reduced levels of ROS and increased survival of oxygen-sensitive commensal bacteria (Faecalibacterium prausnitzii and Roseburia intestinalis). CONCLUSIONS: Mice with hepatocytes that express hREG3A, which travels to the intestinal lumen, are less sensitive to colitis than control mice. We found hREG3A to alter the colonic microbiota by decreasing levels of ROS. Fecal microbiota from REG3A-TG mice protect non-TG mice from induction of colitis. These findings indicate a role for reduction of oxidative stress in preserving the gut microbiota and its ability to prevent inflammation.}, language = {eng}, number = {4}, journal = {Gastroenterology}, author = {Darnaud, Marion and Dos Santos, Alexandre and Gonzalez, Patrick and Augui, Sandrine and Lacoste, Claire and Desterke, Christophe and De Hertogh, Gert and Valentino, Emma and Braun, Emilie and Zheng, Jinzi and Boisgard, Raphael and Neut, Christel and Dubuquoy, Laurent and Chiappini, Franck and Samuel, Didier and Lepage, Patricia and Guerrieri, Francesca and Doré, Joel and Bréchot, Christian and Moniaux, Nicolas and Faivre, Jamila}, year = {2018}, pmid = {29133078}, keywords = {Animals, Bacteria, Colitis, Colon, Dextran Sulfate, Disease Models, Animal, Fecal Microbiota Transplantation, Gastrointestinal Microbiome, Hepatocytes, Humans, IBD, LPS, Mice, Inbred C57BL, Mice, Transgenic, Microbial Viability, Mouse Model, Oxidative Stress, Pancreatitis-Associated Proteins, Reactive Oxygen Species, Time Factors, Trinitrobenzenesulfonic Acid}, pages = {1009--1023.e14}, }
@article{lucchese_neoadjuvant_2018, title = {Neoadjuvant chemotherapy response influences outcomes in non-colorectal, non-neuroendocrine liver metastases}, issn = {1365-2168}, doi = {10.1002/bjs.10884}, abstract = {BACKGROUND: Indications for surgical resection of non-colorectal, non-neuroendocrine (NCNNE) liver metastases are unclear. This study analysed the influence of response to neoadjuvant chemotherapy and the presence of extrahepatic disease (EHD) on outcomes. METHODS: Patients who underwent hepatic resection for NCNNE liver metastases and who received neoadjuvant chemotherapy at a single centre between 1982 and 2016 were analysed retrospectively. Patients were classified as having no EHD, controlled EHD or non-controlled EHD. RESULTS: Hepatic resection was performed in 199 patients (81·2 per cent) after partial or complete response to chemotherapy or disease stabilization, and 46 patients (18·8 per cent) after tumour progression. Patients with progressive disease after chemotherapy had worse overall survival than those without (23 versus 50·4 per cent at 5 years; P = 0·004). Median survival was 63·6 (range 31·1-94·8) months for patients without EHD, 34·8 (19·2-49·2) months for those with controlled EHD and 7·2 (1·2-13·2) months for patients with non-controlled EHD (P = 0·004). In multivariable analysis, EHD (P = 0·004), response to chemotherapy (P = 0·004) and resection margins (P = 0·002) were all independent predictors of overall survival, regardless of primary tumour site. CONCLUSION: The prognosis of patients with NCNNE liver metastases is influenced by preoperative chemotherapy and resectability.}, language = {eng}, journal = {The British Journal of Surgery}, author = {Lucchese, A. M. and Kalil, A. N. and Ruiz, A. and Karam, V. and Ciacio, O. and Pittau, G. and Castaing, D. and Cherqui, D. and Sa Cunha, A. and Vibert, E. and Adam, R.}, month = jun, year = {2018}, pmid = {29893476}, }
@article{machover_enhancement_2018, title = {Enhancement of 5-{Fluorouracil} cytotoxicity by {Pyridoxal} 5'-phosphate and {Folinic} acid in tandem}, issn = {1521-0103}, doi = {10.1124/jpet.118.249367}, abstract = {The present study originates from the assumption that, in tumors, levels of naturally occurring pyridoxal 5'-phosphate (PLP) are too small to allow conversion of tetra hydro pteroylglutamate (H4PteGlu) into methylene tetra hydro pteroylglutamate (CH2-H4PteGlu) in amounts required to improve inhibition of thymidylate synthase by 5-fluorouracil (FUra) through ternary complex stabilization. The hypothesis relates to the low affinity for cofactor of the PLP-dependent serine hydroxymethyl transferase (SHMT), the enzyme that catalyses formation CH2-H4PteGlu by transfer of the Cβ of serine to H4PteGlu. Intracellular concentrations of PLP are smaller than the dissociation constant (KD) of SHMT for cofactor, which suggests that enzyme activity should be sensitive to PLP level changes. Three cancer cell lines were supplemented with PLP to investigate the influence of this cofactor on FUra cytotoxicity. Cells were exposed to FUra, FUra and folinic acid (FA), FUra and PLP, and FUra combined with both FA and PLP. The Median effect principle for concentration-effect analysis and combination indices were used to determine interactions on cytotoxicity. FUra cytotoxicity in vitro was enhanced by FA and PLP in tandem. Synergistic cytotoxic interaction of FUra with FA and PLP was demonstrated in HT29, and L1210 cells. Summation was found in HCT116 cells. Parenteral pyridoxamine was administered in mice to explore erythrocyte production of PLP in vivo. Cofactor attained levels in the range of the KD for binding to SHMT and it was rapidly cleared from cells. Pharmacokinetics of pyridoxamine suggests that modulation of FUra by vitamin B6 could be achieved in vivo.}, language = {eng}, journal = {The Journal of Pharmacology and Experimental Therapeutics}, author = {Machover, David and Goldschmidt, Emma L. and Mollicone, Rosella and Haghighi-Rad, Farhad and Desterke, Christophe and Gaston-Mathe, Yann and Saffroy, Raphael and Boucheix, Claude and Dairou, Julien}, month = jun, year = {2018}, pmid = {29858389}, keywords = {anticancer agents, cancer chemotherapy, chemotherapy, drug development, drug efficacy, drug interactions, vitamins}, }
@article{li_identification_2018, title = {Identification of novel {HLA}-{A11}-restricted {T}-cell epitopes in the {Ebola} virus nucleoprotein}, issn = {1769-714X}, doi = {10.1016/j.micinf.2018.04.005}, abstract = {The Ebola virus (EBOV) is a very contagious virus that is highly fatal in humans and animals. The largest epidemic was in West Africa in 2014, in which over 11,000 people died. However, to date, there are no licensed vaccines against it. Studies show that CD4+ and CD8+ T-cell responses, especially cytotoxic T-lymphocyte (CTL) responses, play key roles in protecting individuals from EBOV infection. Since HLA-restricted epitope vaccines are likely to be effective and safe immunization strategies for infectious diseases, the present study screened for CTL epitopes in the EBOV-nucleoprotein that are restricted by HLA-A11 (a common allele in Chinese people). Predictive computer analysis of the amino-acid sequence of EBOV-nucleoprotein identified ten putative HLA-A11-restricted epitopes. ELISPOT assay of immunized HLA-A11/DR1 transgenic mice showed that five (GR-9, VR-9, EK-9, PK-9, and RK-9) induced effective CTL responses. Additional epitope analyses will aid the design of epitope vaccines against EBOV.}, language = {eng}, journal = {Microbes and Infection}, author = {Li, Dan and Li, Pei and Song, Nianping and Jiang, Yuting and Zeng, Yang and Zhao, Guangyu and Fa, Yunzhi and Ye, Huahu and Lone, Yuchun and Zhou, Yusen and Sun, Shihui and Zeng, Lin}, month = may, year = {2018}, pmid = {29775667}, keywords = {Ebola virus, HLA-A11-restricted epitope, Vaccine}, }