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\n\n \n \n Asai, M., Miyazawa, H., Yanase, R., Inaba, K., & Nakano, H.\n\n\n \n \n \n \n \n A new species of Acoela possessing a middorsal appendage with a possible sensory function.\n \n \n \n \n\n\n \n\n\n\n
Zoological Science, 39(1): 147–156. January 2022.\n
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@article{asai_new_2022,\n\ttitle = {A new species of {Acoela} possessing a middorsal appendage with a possible sensory function},\n\tvolume = {39},\n\tissn = {0289-0003},\n\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210058/A-New-Species-of-Acoela-Possessing-a-Middorsal-Appendage-with/10.2108/zs210058.full},\n\tdoi = {10.2108/zs210058},\n\tnumber = {1},\n\turldate = {2022-01-25},\n\tjournal = {Zoological Science},\n\tauthor = {Asai, Masashi and Miyazawa, Hideyuki and Yanase, Ryuji and Inaba, Kazuo and Nakano, Hiroaki},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {147--156},\n}\n\n\n
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\n\n \n \n Chacha, P. P., Horie, R., Kusakabe, T. G., Sasakura, Y., Singh, M., Horie, T., & Levine, M.\n\n\n \n \n \n \n \n Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate.\n \n \n \n \n\n\n \n\n\n\n
Proceedings of the National Academy of Sciences, 119(4): e2118817119. January 2022.\n
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@article{chacha_neuronal_2022,\n\ttitle = {Neuronal identities derived by misexpression of the {POU} {IV} sensory determinant in a protovertebrate},\n\tvolume = {119},\n\turl = {http://www.pnas.org/content/119/4/e2118817119.abstract},\n\tdoi = {10.1073/pnas.2118817119},\n\tabstract = {The protovertebrate Ciona intestinalis is an ideal system to investigate both gene regulatory networks that underlie cell-type specification and how cell types have evolved. In this study, we use single-cell technology, experimental manipulations, and computational analyses to understand the role of the regulatory determinant POU IV—a homolog of Brn3 in vertebrates—in specifying various sensory cell types in Ciona. Surprisingly, the misexpression of POU IV throughout the epidermis led to the formation of hybrid sensory cell types, including those exhibiting properties of both palp sensory cells and bipolar tail neurons. These results demonstrate the interconnectedness of diverse sensory specification networks and give insights into the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.The protovertebrate Ciona intestinalis type A (sometimes called Ciona robusta) contains a series of sensory cell types distributed across the head–tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant POU IV/Brn3 is known to work in concert with regional determinants, such as Foxg and Neurogenin, to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of POU IV results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of Foxg and Neurogenin that is triggered by an unexpected POU IV feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.All study data are included in the article and/or SI Appendix. All code, analyses, and files can be accessed from the GitHub repository https://github.com/Singh-Lab/Pou4\\_Misexpression. In addition, the data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus (Chacha et al., 2021) and are accessible through GEO Series accession number GSE192645 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE192645).},\n\tnumber = {4},\n\tjournal = {Proceedings of the National Academy of Sciences},\n\tauthor = {Chacha, Prakriti Paul and Horie, Ryoko and Kusakabe, Takehiro G. and Sasakura, Yasunori and Singh, Mona and Horie, Takeo and Levine, Michael},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {e2118817119},\n}\n\n\n
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\n The protovertebrate Ciona intestinalis is an ideal system to investigate both gene regulatory networks that underlie cell-type specification and how cell types have evolved. In this study, we use single-cell technology, experimental manipulations, and computational analyses to understand the role of the regulatory determinant POU IV—a homolog of Brn3 in vertebrates—in specifying various sensory cell types in Ciona. Surprisingly, the misexpression of POU IV throughout the epidermis led to the formation of hybrid sensory cell types, including those exhibiting properties of both palp sensory cells and bipolar tail neurons. These results demonstrate the interconnectedness of diverse sensory specification networks and give insights into the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.The protovertebrate Ciona intestinalis type A (sometimes called Ciona robusta) contains a series of sensory cell types distributed across the head–tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant POU IV/Brn3 is known to work in concert with regional determinants, such as Foxg and Neurogenin, to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of POU IV results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of Foxg and Neurogenin that is triggered by an unexpected POU IV feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.All study data are included in the article and/or SI Appendix. All code, analyses, and files can be accessed from the GitHub repository https://github.com/Singh-Lab/Pou4_Misexpression. In addition, the data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus (Chacha et al., 2021) and are accessible through GEO Series accession number GSE192645 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE192645).\n
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\n\n \n \n Chiba, M., Hirano, T., Yamazaki, D., Ye, B., Ito, S., Kagawa, O., Endo, K., Nishida, S., Hara, S., Aratake, K., & Chiba, S.\n\n\n \n \n \n \n \n The mutual history of Schlegel’s Japanese gecko (Reptilia: Squamata: Gekkonidae) and humans inscribed in genes and ancient literature.\n \n \n \n \n\n\n \n\n\n\n
PNAS Nexus, 1(5): pgac245. November 2022.\n
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@article{chiba_mutual_2022,\n\ttitle = {The mutual history of {Schlegel}’s {Japanese} gecko ({Reptilia}: {Squamata}: {Gekkonidae}) and humans inscribed in genes and ancient literature},\n\tvolume = {1},\n\tissn = {2752-6542},\n\tshorttitle = {The mutual history of {Schlegel}’s {Japanese} gecko ({Reptilia}},\n\turl = {https://academic.oup.com/pnasnexus/article/doi/10.1093/pnasnexus/pgac245/6847526},\n\tdoi = {10.1093/pnasnexus/pgac245},\n\tabstract = {Abstract\n Knowing how the present distribution of organisms was formed is an essential issue in evolutionary ecology. Recently, the distribution of organisms on Earth has been significantly changed by human-mediated dispersal due to globalization. Therefore, significant attention has been paid to such processes. However, although humankind has taken considerable time to achieve modernization, the impact of ancient human activity on ecosystems has not yet been thoroughly studied. We hypothesized that ancient urban development and transitions had a non-negligible effect on species distribution. Inferring the impact of past human activity on ecosystems from ancient literature and verifying that impact by genetic analysis and human history is an effective means of tackling this problem. As geckos, a popular neighbor of human dwellings, are good material for this model, we performed this combination approach using Schlegel’s Japanese gecko, Gekko japonicus. We show that G. japonicus migrated from China to the western Japanese archipelago before Christ. The gecko species dispersed itself from western to eastern the archipelago on a time scale of thousands of years. There are many synchronizations between the dispersal history of G. japonicus and the historical development of human society. It is suggested by such synchronizations that humans have influenced the distribution of G. japonicus many times throughout its dispersal history.},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2023-07-07},\n\tjournal = {PNAS Nexus},\n\tauthor = {Chiba, Minoru and Hirano, Takahiro and Yamazaki, Daishi and Ye, Bin and Ito, Shun and Kagawa, Osamu and Endo, Komei and Nishida, Shu and Hara, Seiji and Aratake, Kenichiro and Chiba, Satoshi},\n\teditor = {Ma, Li-Jun},\n\tmonth = nov,\n\tyear = {2022},\n\tkeywords = {InabaKen},\n\tpages = {pgac245},\n}\n\n\n
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\n Abstract Knowing how the present distribution of organisms was formed is an essential issue in evolutionary ecology. Recently, the distribution of organisms on Earth has been significantly changed by human-mediated dispersal due to globalization. Therefore, significant attention has been paid to such processes. However, although humankind has taken considerable time to achieve modernization, the impact of ancient human activity on ecosystems has not yet been thoroughly studied. We hypothesized that ancient urban development and transitions had a non-negligible effect on species distribution. Inferring the impact of past human activity on ecosystems from ancient literature and verifying that impact by genetic analysis and human history is an effective means of tackling this problem. As geckos, a popular neighbor of human dwellings, are good material for this model, we performed this combination approach using Schlegel’s Japanese gecko, Gekko japonicus. We show that G. japonicus migrated from China to the western Japanese archipelago before Christ. The gecko species dispersed itself from western to eastern the archipelago on a time scale of thousands of years. There are many synchronizations between the dispersal history of G. japonicus and the historical development of human society. It is suggested by such synchronizations that humans have influenced the distribution of G. japonicus many times throughout its dispersal history.\n
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\n\n \n \n Hall-Spencer, J. M., Belfiore, G., Tomatsuri, M., Porzio, L., Harvey, B. P., Agostini, S., & Kon, K.\n\n\n \n \n \n \n \n Decreased diversity and abundance of marine invertebrates at CO$_{\\textrm{2}}$ seeps in warm-temperate Japan.\n \n \n \n \n\n\n \n\n\n\n
Zoological Science, 39(1): 41–51. January 2022.\n
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@article{hall-spencer_decreased_2022,\n\ttitle = {Decreased diversity and abundance of marine invertebrates at {CO}$_{\\textrm{2}}$ seeps in warm-temperate {Japan}},\n\tvolume = {39},\n\tissn = {0289-0003},\n\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210061/Decreased-Diversity-and-Abundance-of-Marine-Invertebrates-at-CO2-Seeps/10.2108/zs210061.full},\n\tdoi = {10.2108/zs210061},\n\tnumber = {1},\n\turldate = {2022-02-02},\n\tjournal = {Zoological Science},\n\tauthor = {Hall-Spencer, Jason M. and Belfiore, Giuseppe and Tomatsuri, Morihiko and Porzio, Lucia and Harvey, Ben P. and Agostini, Sylvain and Kon, Koetsu},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {41--51},\n}\n\n\n
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\n\n \n \n Harvey, B. P., Marshall, K. E., Harley, C. D., & Russell, B. D.\n\n\n \n \n \n \n \n Predicting responses to marine heatwaves using functional traits.\n \n \n \n \n\n\n \n\n\n\n
Trends in Ecology & Evolution, 37(1): 20–29. January 2022.\n
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@article{harvey_predicting_2022,\n\ttitle = {Predicting responses to marine heatwaves using functional traits},\n\tvolume = {37},\n\tissn = {01695347},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0169534721002500},\n\tdoi = {10.1016/j.tree.2021.09.003},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-12-21},\n\tjournal = {Trends in Ecology \\& Evolution},\n\tauthor = {Harvey, Ben P. and Marshall, Katie E. and Harley, Christopher D.G. and Russell, Bayden D.},\n\tmonth = jan,\n\tyear = {2022},\n\tkeywords = {climate change, marine heatwaves, trait-based ecology},\n\tpages = {20--29},\n}\n\n
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\n\n \n \n Hazraty-Kari, S., Tavakoli-Kolour, P., Kitanobo, S., Nakamura, T., & Morita, M.\n\n\n \n \n \n \n \n Adaptations by the coral Acropora tenuis confer resilience to future thermal stress.\n \n \n \n \n\n\n \n\n\n\n
Communications Biology, 5(1): 1371. December 2022.\n
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@article{hazraty-kari_adaptations_2022,\n\ttitle = {Adaptations by the coral {Acropora} tenuis confer resilience to future thermal stress},\n\tvolume = {5},\n\tissn = {2399-3642},\n\turl = {https://www.nature.com/articles/s42003-022-04309-5},\n\tdoi = {10.1038/s42003-022-04309-5},\n\tabstract = {Abstract\n \n Elevated temperatures cause coral bleaching and reef degradation. However, coral may have strategies to survive by reproducing more heat-tolerable larvae. We examine the direct and carryover effects of thermal stress on fecundity and fitness in the reef-building coral\n Acropora tenuis\n . Fragments from the same colony are subjected to control temperature ({\\textasciitilde}27.5 °C) or heat stress ({\\textasciitilde}31 °C) for ten days. We then examine the fecundity of adults (egg number and size) and the thermal tolerance of larvae and recruits (survival rates, growth, and size). The stressed fragments show a trade-off in egg production, an increase in egg number but a decrease in size. In addition, larvae and recruits from the stressed colony show marginally higher survival rates in the higher water temperature but do not differ in the control condition. Therefore, corals produce more heat-resistant larvae and recruits after they experience heat stress, which may improve coral reef resilience.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2023-07-07},\n\tjournal = {Communications Biology},\n\tauthor = {Hazraty-Kari, Sanaz and Tavakoli-Kolour, Parviz and Kitanobo, Seiya and Nakamura, Takashi and Morita, Masaya},\n\tmonth = dec,\n\tyear = {2022},\n\tkeywords = {InabaKen},\n\tpages = {1371},\n}\n\n\n
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\n Abstract Elevated temperatures cause coral bleaching and reef degradation. However, coral may have strategies to survive by reproducing more heat-tolerable larvae. We examine the direct and carryover effects of thermal stress on fecundity and fitness in the reef-building coral Acropora tenuis . Fragments from the same colony are subjected to control temperature (~27.5 °C) or heat stress (~31 °C) for ten days. We then examine the fecundity of adults (egg number and size) and the thermal tolerance of larvae and recruits (survival rates, growth, and size). The stressed fragments show a trade-off in egg production, an increase in egg number but a decrease in size. In addition, larvae and recruits from the stressed colony show marginally higher survival rates in the higher water temperature but do not differ in the control condition. Therefore, corals produce more heat-resistant larvae and recruits after they experience heat stress, which may improve coral reef resilience.\n
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\n\n \n \n Ito, S., Yamazaki, D., Kameda, Y., Kagawa, O., Ye, B., Saito, T., Kimura, K., Do, V. T., Chiba, S., & Hirano, T.\n\n\n \n \n \n \n \n Taxonomic insights and evolutionary history in East Asian terrestrial slugs of the genus Meghimatium.\n \n \n \n \n\n\n \n\n\n\n
Molecular Phylogenetics and Evolution, 182: 107730. May 2023.\n
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@article{ito_taxonomic_2023,\n\ttitle = {Taxonomic insights and evolutionary history in {East} {Asian} terrestrial slugs of the genus \\textit{{Meghimatium}}},\n\tvolume = {182},\n\tissn = {10557903},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S1055790323000301},\n\tdoi = {10.1016/j.ympev.2023.107730},\n\tlanguage = {en},\n\turldate = {2023-07-07},\n\tjournal = {Molecular Phylogenetics and Evolution},\n\tauthor = {Ito, Shun and Yamazaki, Daishi and Kameda, Yuichi and Kagawa, Osamu and Ye, Bin and Saito, Takumi and Kimura, Kazuki and Do, Van Tu and Chiba, Satoshi and Hirano, Takahiro},\n\tmonth = may,\n\tyear = {2023},\n\tkeywords = {InabaKen},\n\tpages = {107730},\n}\n\n\n
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\n\n \n \n Ito, T., Morita, M., Okuno, S., Inaba, K., Shiba, K., Munehara, H., Koya, Y., Homma, M., & Awata, S.\n\n\n \n \n \n \n \n Fertilization modes and the evolution of sperm characteristics in marine fishes: Paired comparisons of externally and internally fertilizing species.\n \n \n \n \n\n\n \n\n\n\n
Ecology and Evolution, 12(12). December 2022.\n
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@article{ito_fertilization_2022,\n\ttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes: {Paired} comparisons of externally and internally fertilizing species},\n\tvolume = {12},\n\tissn = {2045-7758, 2045-7758},\n\tshorttitle = {Fertilization modes and the evolution of sperm characteristics in marine fishes},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/ece3.9562},\n\tdoi = {10.1002/ece3.9562},\n\tlanguage = {en},\n\tnumber = {12},\n\turldate = {2022-12-07},\n\tjournal = {Ecology and Evolution},\n\tauthor = {Ito, Takeshi and Morita, Masaya and Okuno, Seiya and Inaba, Kazuo and Shiba, Kogiku and Munehara, Hiroyuki and Koya, Yasunori and Homma, Mitsuo and Awata, Satoshi},\n\tmonth = dec,\n\tyear = {2022},\n}\n\n\n
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\n\n \n \n Jokura, K., Sato, Y., Shiba, K., & Inaba, K.\n\n\n \n \n \n \n \n Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia.\n \n \n \n \n\n\n \n\n\n\n
Current Biology,S0960982222016037. October 2022.\n
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@article{jokura_two_2022,\n\ttitle = {Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia},\n\tissn = {09609822},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0960982222016037},\n\tdoi = {10.1016/j.cub.2022.09.061},\n\tlanguage = {en},\n\turldate = {2022-10-25},\n\tjournal = {Current Biology},\n\tauthor = {Jokura, Kei and Sato, Yu and Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = oct,\n\tyear = {2022},\n\tpages = {S0960982222016037},\n}\n\n\n
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\n\n \n \n Kerfahi, D., Harvey, B. P., Kim, H., Yang, Y., Adams, J. M., & Hall-Spencer, J. M.\n\n\n \n \n \n \n \n Whole community and functional gene changes of biofilms on marine plastic debris in response to ocean acidification.\n \n \n \n \n\n\n \n\n\n\n
Microbial Ecology, 85(4): 1202–1214. April 2022.\n
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@article{kerfahi_whole_2022,\n\ttitle = {Whole community and functional gene changes of biofilms on marine plastic debris in response to ocean acidification},\n\tvolume = {85},\n\tissn = {0095-3628, 1432-184X},\n\turl = {https://link.springer.com/10.1007/s00248-022-01987-w},\n\tdoi = {10.1007/s00248-022-01987-w},\n\tlanguage = {en},\n\tnumber = {4},\n\turldate = {2023-03-09},\n\tjournal = {Microbial Ecology},\n\tauthor = {Kerfahi, Dorsaf and Harvey, Ben P. and Kim, Hyoki and Yang, Ying and Adams, Jonathan M. and Hall-Spencer, Jason M.},\n\tmonth = apr,\n\tyear = {2022},\n\tpages = {1202--1214},\n}\n\n\n
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\n\n \n \n Khalturin, K., Shunatova, N., Shchenkov, S., Sasakura, Y., Kawamitsu, M., & Satoh, N.\n\n\n \n \n \n \n \n Polyzoa is back: The effect of complete gene sets on the placement of Ectoprocta and Entoprocta.\n \n \n \n \n\n\n \n\n\n\n
Science Advances, 8(26): eabo4400. July 2022.\n
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@article{khalturin_polyzoa_2022,\n\ttitle = {Polyzoa is back: {The} effect of complete gene sets on the placement of {Ectoprocta} and {Entoprocta}},\n\tvolume = {8},\n\tissn = {2375-2548},\n\tshorttitle = {Polyzoa is back},\n\turl = {https://www.science.org/doi/10.1126/sciadv.abo4400},\n\tdoi = {10.1126/sciadv.abo4400},\n\tabstract = {The phylogenomic approach has largely resolved metazoan phylogeny and improved our knowledge of animal evolution based on morphology, paleontology, and embryology. Nevertheless, the placement of two major lophotrochozoan phyla, Entoprocta (Kamptozoa) and Ectoprocta (Bryozoa), remains highly controversial: Originally considered as a single group named Polyzoa (Bryozoa), they were separated on the basis of morphology. So far, each new study of lophotrochozoan evolution has still consistently proposed different phylogenetic positions for these groups. Here, we reinvestigated the placement of Entoprocta and Ectoprocta using highly complete datasets with rigorous contamination removal. Our results from maximum likelihood, Bayesian, and coalescent analyses strongly support the topology in which Entoprocta and Bryozoa form a distinct clade, placed as a sister group to all other lophotrochozoan clades: Annelida, Mollusca, Brachiopoda, Phoronida, and Nemertea. Our study favors the evolutionary scenario where Entoprocta, Cycliophora, and Bryozoa constitute one of the earliest branches among Lophotrochozoa and thus supports the Polyzoa hypothesis.\n , \n The earliest lineage among the Lophotrochozoa is identified.},\n\tlanguage = {en},\n\tnumber = {26},\n\turldate = {2022-11-01},\n\tjournal = {Science Advances},\n\tauthor = {Khalturin, Konstantin and Shunatova, Natalia and Shchenkov, Sergei and Sasakura, Yasunori and Kawamitsu, Mayumi and Satoh, Noriyuki},\n\tmonth = jul,\n\tyear = {2022},\n\tpages = {eabo4400},\n}\n\n\n
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\n The phylogenomic approach has largely resolved metazoan phylogeny and improved our knowledge of animal evolution based on morphology, paleontology, and embryology. Nevertheless, the placement of two major lophotrochozoan phyla, Entoprocta (Kamptozoa) and Ectoprocta (Bryozoa), remains highly controversial: Originally considered as a single group named Polyzoa (Bryozoa), they were separated on the basis of morphology. So far, each new study of lophotrochozoan evolution has still consistently proposed different phylogenetic positions for these groups. Here, we reinvestigated the placement of Entoprocta and Ectoprocta using highly complete datasets with rigorous contamination removal. Our results from maximum likelihood, Bayesian, and coalescent analyses strongly support the topology in which Entoprocta and Bryozoa form a distinct clade, placed as a sister group to all other lophotrochozoan clades: Annelida, Mollusca, Brachiopoda, Phoronida, and Nemertea. Our study favors the evolutionary scenario where Entoprocta, Cycliophora, and Bryozoa constitute one of the earliest branches among Lophotrochozoa and thus supports the Polyzoa hypothesis. , The earliest lineage among the Lophotrochozoa is identified.\n
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\n\n \n \n Kinjo, S., Kiyomoto, M., Suzuki, H., Yamamoto, T., Ikeo, K., & Yaguchi, S.\n\n\n \n \n \n \n \n TrBase: A genome and transcriptome database of Temnopleurus reevesii.\n \n \n \n \n\n\n \n\n\n\n
Development, Growth & Differentiation, 64(4): 210–218. May 2022.\n
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@article{kinjo_trbase_2022,\n\ttitle = {{TrBase}: {A} genome and transcriptome database of \\textit{{Temnopleurus} reevesii}},\n\tvolume = {64},\n\tissn = {0012-1592, 1440-169X},\n\tshorttitle = {{\\textless}span style="font-variant},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12780},\n\tdoi = {10.1111/dgd.12780},\n\tlanguage = {en},\n\tnumber = {4},\n\turldate = {2023-07-07},\n\tjournal = {Development, Growth \\& Differentiation},\n\tauthor = {Kinjo, Sonoko and Kiyomoto, Masato and Suzuki, Haruka and Yamamoto, Takashi and Ikeo, Kazuho and Yaguchi, Shunsuke},\n\tmonth = may,\n\tyear = {2022},\n\tpages = {210--218},\n}\n\n\n
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\n\n \n \n Kudo, K., Kagawa, O., Ito, S., Wada, S., Nishi, H., Shariar, S., Yamazaki, D., Hirano, T., & Chiba, S.\n\n\n \n \n \n \n \n Species identification and invasion pathways of an introduced snail Macrochlamys sp. in Japan.\n \n \n \n \n\n\n \n\n\n\n
BioInvasions Records, 11(4): 839–854. 2022.\n
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@article{kudo_species_2022,\n\ttitle = {Species identification and invasion pathways of an introduced snail \\textit{{Macrochlamys}} sp. in {Japan}},\n\tvolume = {11},\n\tissn = {22421300},\n\turl = {https://www.reabic.net/journals/bir/2022/Issue4.aspx},\n\tdoi = {10.3391/bir.2022.11.4.03},\n\tnumber = {4},\n\turldate = {2023-07-07},\n\tjournal = {BioInvasions Records},\n\tauthor = {Kudo, Kodai and Kagawa, Osamu and Ito, Shun and Wada, Shinichiro and Nishi, Hirotaka and Shariar, Shovon and Yamazaki, Daishi and Hirano, Takahiro and Chiba, Satoshi},\n\tyear = {2022},\n\tkeywords = {InabaKen, wrongWada},\n\tpages = {839--854},\n}\n\n\n
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\n\n \n \n Kushida, Y., Imahara, Y., Wee, H. B., Fernandez-Silva, I., Fromont, J., Gomez, O., Wilson, N., Kimura, T., Tsuchida, S., Fujiwara, Y., Higashiji, T., Nakano, H., Kohtsuka, H., Iguchi, A., & Reimer, J. D.\n\n\n \n \n \n \n \n Exploring the trends of adaptation and evolution of sclerites with regards to habitat depth in sea pens.\n \n \n \n \n\n\n \n\n\n\n
PeerJ, 10: e13929. September 2022.\n
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\n\n \n \n Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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@article{kushida_exploring_2022,\n\ttitle = {Exploring the trends of adaptation and evolution of sclerites with regards to habitat depth in sea pens},\n\tvolume = {10},\n\tissn = {2167-8359},\n\turl = {https://peerj.com/articles/13929},\n\tdoi = {10.7717/peerj.13929},\n\tabstract = {Octocorals possess sclerites, small elements comprised of calcium carbonate (CaCO\n 3\n ) that are important diagnostic characters in octocoral taxonomy. Among octocorals, sea pens comprise a unique order (Pennatulacea) that live in a wide range of depths. Habitat depth is considered to be important in the diversification of octocoral species, but a lack of information on sea pens has limited studies on their adaptation and evolution across depth. Here, we aimed to reveal trends of adaptation and evolution of sclerite shapes in sea pens with regards to habitat depth\n via\n phylogenetic analyses and ancestral reconstruction analyses. Colony form of sea pens is suggested to have undergone convergent evolution and the loss of axis has occurred independently across the evolution of sea pens. Divergences of sea pen taxa and of sclerite forms are suggested to depend on habitat depths. In addition, their sclerite forms may be related to evolutionary history of the sclerite and the surrounding chemical environment as well as water temperature. Three-flanged sclerites may possess the tolerance towards the environment of the deep sea, while plate sclerites are suggested to be adapted towards shallower waters, and have evolved independently multiple times. The common ancestor form of sea pens was predicted to be deep-sea and similar to family Pseudumbellulidae in form, possessing sclerites intermediate in form to those of alcyonaceans and modern sea pens such as spindles, rods with spines, and three-flanged sclerites with serrated edges sclerites, as well as having an axis and bilateral traits.},\n\tlanguage = {en},\n\turldate = {2022-10-31},\n\tjournal = {PeerJ},\n\tauthor = {Kushida, Yuka and Imahara, Yukimitsu and Wee, Hin Boo and Fernandez-Silva, Iria and Fromont, Jane and Gomez, Oliver and Wilson, Nerida and Kimura, Taeko and Tsuchida, Shinji and Fujiwara, Yoshihiro and Higashiji, Takuo and Nakano, Hiroaki and Kohtsuka, Hisanori and Iguchi, Akira and Reimer, James Davis},\n\tmonth = sep,\n\tyear = {2022},\n\tpages = {e13929},\n}\n\n\n
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\n Octocorals possess sclerites, small elements comprised of calcium carbonate (CaCO 3 ) that are important diagnostic characters in octocoral taxonomy. Among octocorals, sea pens comprise a unique order (Pennatulacea) that live in a wide range of depths. Habitat depth is considered to be important in the diversification of octocoral species, but a lack of information on sea pens has limited studies on their adaptation and evolution across depth. Here, we aimed to reveal trends of adaptation and evolution of sclerite shapes in sea pens with regards to habitat depth via phylogenetic analyses and ancestral reconstruction analyses. Colony form of sea pens is suggested to have undergone convergent evolution and the loss of axis has occurred independently across the evolution of sea pens. Divergences of sea pen taxa and of sclerite forms are suggested to depend on habitat depths. In addition, their sclerite forms may be related to evolutionary history of the sclerite and the surrounding chemical environment as well as water temperature. Three-flanged sclerites may possess the tolerance towards the environment of the deep sea, while plate sclerites are suggested to be adapted towards shallower waters, and have evolved independently multiple times. The common ancestor form of sea pens was predicted to be deep-sea and similar to family Pseudumbellulidae in form, possessing sclerites intermediate in form to those of alcyonaceans and modern sea pens such as spindles, rods with spines, and three-flanged sclerites with serrated edges sclerites, as well as having an axis and bilateral traits.\n
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\n\n \n \n Mutalipassi, M., Mazzella, V., Schott, M., Fink, P., Glaviano, F., Porzio, L., Lorenti, M., Buia, M. C., Von Elert, E., & Zupo, V.\n\n\n \n \n \n \n \n Ocean Acidification Affects Volatile Infochemicals Production and Perception in Fauna and Flora Associated With Posidonia oceanica (L.) Delile.\n \n \n \n \n\n\n \n\n\n\n
Frontiers in Marine Science, 9: 809702. March 2022.\n
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@article{mutalipassi_ocean_2022,\n\ttitle = {Ocean {Acidification} {Affects} {Volatile} {Infochemicals} {Production} and {Perception} in {Fauna} and {Flora} {Associated} {With} {Posidonia} oceanica ({L}.) {Delile}},\n\tvolume = {9},\n\tissn = {2296-7745},\n\turl = {https://www.frontiersin.org/articles/10.3389/fmars.2022.809702/full},\n\tdoi = {10.3389/fmars.2022.809702},\n\tabstract = {Communication among marine organisms are generally based on production, transmission, and interpretation of chemical cues. Volatile organic compounds (VOCs) can act as infochemicals, and ocean acidification can alter their production in the source organisms as well as the interpretation of the information they drive to target organisms. Two diatoms (\n Cocconeis scutellum\n var.\n parva\n and\n Diploneis\n sp.) and a macroalga (\n Ulva prolifera\n ), all common epiphytes of\n Posidonia oceanica\n leaves, were isolated and cultured at two pH conditions (8.2 and 7.7). Their biomass was collected, and the VOCs produced upon wounding were extracted and analyzed using gas chromatography. Chemotactic reactions of invertebrates triggered by VOCs were tested using a static choice experimental arena and a flow-through flume system. Odor choice experiments were performed on several invertebrates associated with\n P. oceanica\n meadows to investigate the modification of behavioral responses due to the growth of algae in acidified environments. Complex patterns of behavioral responses were recorded after exposure to algal VOCs. This study demonstrated that a) ocean acidification alters the bouquet of VOCs released by diatoms and macroalgae and b) these compounds act as infochemicals and trigger peculiar behavioral responses in benthic invertebrates. In addition, behavioral responses are species-specific, dose-dependent, and are modified by environmental constraints. In fact, the static diffusion in choice arenas produced different responses as compared to flow-through flume systems. In conclusion, we demonstrate that in future marine environments higher CO\n 2\n concentrations (leading to a pH 7.7 by the end of this century) will modify the production of VOCs by micro- and macroalgae as well as the recognition of these infochemicals by marine invertebrates.},\n\turldate = {2023-06-13},\n\tjournal = {Frontiers in Marine Science},\n\tauthor = {Mutalipassi, Mirko and Mazzella, Valerio and Schott, Matthias and Fink, Patrick and Glaviano, Francesca and Porzio, Lucia and Lorenti, Maurizio and Buia, Maria Cristina and Von Elert, Eric and Zupo, Valerio},\n\tmonth = mar,\n\tyear = {2022},\n\tpages = {809702},\n}\n\n\n
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\n Communication among marine organisms are generally based on production, transmission, and interpretation of chemical cues. Volatile organic compounds (VOCs) can act as infochemicals, and ocean acidification can alter their production in the source organisms as well as the interpretation of the information they drive to target organisms. Two diatoms ( Cocconeis scutellum var. parva and Diploneis sp.) and a macroalga ( Ulva prolifera ), all common epiphytes of Posidonia oceanica leaves, were isolated and cultured at two pH conditions (8.2 and 7.7). Their biomass was collected, and the VOCs produced upon wounding were extracted and analyzed using gas chromatography. Chemotactic reactions of invertebrates triggered by VOCs were tested using a static choice experimental arena and a flow-through flume system. Odor choice experiments were performed on several invertebrates associated with P. oceanica meadows to investigate the modification of behavioral responses due to the growth of algae in acidified environments. Complex patterns of behavioral responses were recorded after exposure to algal VOCs. This study demonstrated that a) ocean acidification alters the bouquet of VOCs released by diatoms and macroalgae and b) these compounds act as infochemicals and trigger peculiar behavioral responses in benthic invertebrates. In addition, behavioral responses are species-specific, dose-dependent, and are modified by environmental constraints. In fact, the static diffusion in choice arenas produced different responses as compared to flow-through flume systems. In conclusion, we demonstrate that in future marine environments higher CO 2 concentrations (leading to a pH 7.7 by the end of this century) will modify the production of VOCs by micro- and macroalgae as well as the recognition of these infochemicals by marine invertebrates.\n
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\n\n \n \n Nakano, H.\n\n\n \n \n \n \n Dawn of Placozoan Phylogenetic Taxonomy.\n \n \n \n\n\n \n\n\n\n In 100th Anniversary Publication Committee, Seto Marine Biological Laboratory, & Kyoto University, editor(s),
Marine Invertebrate Biodiversity, pages 28–38. Kyoto University Academic Press, Kyoto, November 2022.\n
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@incollection{nakano_dawn_2022,\n\taddress = {Kyoto},\n\ttitle = {Dawn of {Placozoan} {Phylogenetic} {Taxonomy}},\n\tisbn = {978-4-8140-0449-2},\n\tlanguage = {Japanese},\n\tbooktitle = {Marine {Invertebrate} {Biodiversity}},\n\tpublisher = {Kyoto University Academic Press},\n\tauthor = {Nakano, Hiroaki},\n\teditor = {{100th Anniversary Publication Committee} and {Seto Marine Biological Laboratory} and {Kyoto University}},\n\tmonth = nov,\n\tyear = {2022},\n\tpages = {28--38},\n}\n\n\n
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\n\n \n \n Nakano, H., Isowa, Y., & Inaba, K.\n\n\n \n \n \n \n \n JAMBIO and its coastal organism joint surveys: Network of marine stations explores Japanese coastal biota.\n \n \n \n \n\n\n \n\n\n\n
Zoological Science, 39(1): 1–6. February 2022.\n
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@article{nakano_jambio_2022,\n\ttitle = {{JAMBIO} and its coastal organism joint surveys: {Network} of marine stations explores {Japanese} coastal biota},\n\tvolume = {39},\n\tissn = {0289-0003},\n\tshorttitle = {{JAMBIO} and {Its} {Coastal} {Organism} {Joint} {Surveys}},\n\turl = {https://bioone.org/journals/zoological-science/volume-39/issue-1/zs210069/JAMBIO-and-Its-Coastal-Organism-Joint-Surveys--Network-of/10.2108/zs210069.full},\n\tdoi = {10.2108/zs210069},\n\tnumber = {1},\n\turldate = {2022-02-04},\n\tjournal = {Zoological Science},\n\tauthor = {Nakano, Hiroaki and Isowa, Yukinobu and Inaba, Kazuo},\n\tmonth = feb,\n\tyear = {2022},\n\tpages = {1--6},\n}\n\n\n
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\n\n \n \n Oda, A., Watanabe, H. K., Ohtsuka, S., Wada, S., Kondo, Y., & Miyake, H.\n\n\n \n \n \n \n \n Does the Kuroshio Current transport planktonic larvae of the hydrothermal-vent crab Xenograpsus Takeda & Kurata, 1977 (Decapoda: Brachyura: Grapsoidea)?.\n \n \n \n \n\n\n \n\n\n\n
Journal of Crustacean Biology, 42(1): ruac016. March 2022.\n
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@article{oda_does_2022,\n\ttitle = {Does the {Kuroshio} {Current} transport planktonic larvae of the hydrothermal-vent crab \\textit{{Xenograpsus}} {Takeda} \\& {Kurata}, 1977 ({Decapoda}: {Brachyura}: {Grapsoidea})?},\n\tvolume = {42},\n\tissn = {0278-0372, 1937-240X},\n\tshorttitle = {Does the {Kuroshio} {Current} transport planktonic larvae of the hydrothermal-vent crab \\textit{{Xenograpsus}} {Takeda} \\& {Kurata}, 1977 ({Decapoda}},\n\turl = {https://academic.oup.com/jcb/article/doi/10.1093/jcbiol/ruac016/6546672},\n\tdoi = {10.1093/jcbiol/ruac016},\n\tabstract = {Abstract\n The grapsoid crab XenograpsusTakeda \\& Kurata, 1977 inhabiting shallow-water hydrothermal vents shows a limited distribution in the western Pacific, thus providing a good opportunity to observe how a species expands its distribution and establishes and maintains a metapopulation. We analyzed four Japanese populations of Xenograpsus (Kueishan-dao, Showa Iwo-jima, Shikine-jima, and Omuro-dashi) distributed along the Kuroshio Current to assess whether population connectivity is affected by the current. The reconstructed phylogenetic tree indicated that the specimens analyzed belong to a single clade, including X. testudinatusNg, Huang \\& Ho, 2000 in Kueishan-dao. We could not include genetic data of X. novaeinsularisTakeda \\& Kurata, 1977 and tentatively refer to the specimens analyzed as X. testudinatus. All four populations shared the two major haplotypes. Statistically significant population differentiation was nevertheless recognized between Showa Iwo-jima and Omuro-dashi, about 1,000 km apart. The number of individuals analyzed in Shikine-jima (N = 11) and Kueishan-dao (N = 5) might not be enough to detect differences. The relative age of population expansion seemed to correlate with the direction of the Kuroshio Countercurrent. Together with knowledge on the distribution of the planktonic larvae, larvae of X. testudinatus stay and mostly contribute to its source population. The Kuroshio Current system occasionally transports the larvae and juveniles, or even adult individuals on floating pumice and other materials, to eventually settle into newly formed habitats after volcanic eruptions, thus expanding the distributional range of the species.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2022-09-01},\n\tjournal = {Journal of Crustacean Biology},\n\tauthor = {Oda, Ayako and Watanabe, Hiromi Kayama and Ohtsuka, Susumu and Wada, Shigeki and Kondo, Yusuke and Miyake, Hiroshi},\n\tmonth = mar,\n\tyear = {2022},\n\tpages = {ruac016},\n}\n\n\n
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\n Abstract The grapsoid crab XenograpsusTakeda & Kurata, 1977 inhabiting shallow-water hydrothermal vents shows a limited distribution in the western Pacific, thus providing a good opportunity to observe how a species expands its distribution and establishes and maintains a metapopulation. We analyzed four Japanese populations of Xenograpsus (Kueishan-dao, Showa Iwo-jima, Shikine-jima, and Omuro-dashi) distributed along the Kuroshio Current to assess whether population connectivity is affected by the current. The reconstructed phylogenetic tree indicated that the specimens analyzed belong to a single clade, including X. testudinatusNg, Huang & Ho, 2000 in Kueishan-dao. We could not include genetic data of X. novaeinsularisTakeda & Kurata, 1977 and tentatively refer to the specimens analyzed as X. testudinatus. All four populations shared the two major haplotypes. Statistically significant population differentiation was nevertheless recognized between Showa Iwo-jima and Omuro-dashi, about 1,000 km apart. The number of individuals analyzed in Shikine-jima (N = 11) and Kueishan-dao (N = 5) might not be enough to detect differences. The relative age of population expansion seemed to correlate with the direction of the Kuroshio Countercurrent. Together with knowledge on the distribution of the planktonic larvae, larvae of X. testudinatus stay and mostly contribute to its source population. The Kuroshio Current system occasionally transports the larvae and juveniles, or even adult individuals on floating pumice and other materials, to eventually settle into newly formed habitats after volcanic eruptions, thus expanding the distributional range of the species.\n
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\n\n \n \n Shiba, K., & Inaba, K.\n\n\n \n \n \n \n \n The roles of two CNG channels in the regulation of ascidian sperm chemotaxis.\n \n \n \n \n\n\n \n\n\n\n
International Journal of Molecular Sciences, 23(3): 1648. January 2022.\n
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@article{shiba_roles_2022,\n\ttitle = {The roles of two {CNG} channels in the regulation of ascidian sperm chemotaxis},\n\tvolume = {23},\n\tissn = {1422-0067},\n\turl = {https://www.mdpi.com/1422-0067/23/3/1648},\n\tdoi = {10.3390/ijms23031648},\n\tabstract = {Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2022-02-07},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {1648},\n}\n\n\n
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\n Spermatozoa sense and respond to their environmental signals to ensure fertilization success. Reception and transduction of signals are reflected rapidly in sperm flagellar waveforms and swimming behavior. In the ascidian Ciona intestinalis (type A; also called C. robusta), an egg-derived sulfated steroid called SAAF (sperm activating and attracting factor), induces both sperm motility activation and chemotaxis. Two types of CNG (cyclic nucleotide-gated) channels, Ci-tetra KCNG (tetrameric, cyclic nucleotide-gated, K+-selective) and Ci-HCN (hyperpolarization-activated and cyclic nucleotide-gated), are highly expressed in Ciona testis from the comprehensive gene expression analysis. To elucidate the sperm signaling pathway to regulate flagellar motility, we focus on the role of CNG channels. In this study, the immunochemical analysis revealed that both CNG channels are expressed in Ciona sperm and localized to sperm flagella. Sperm motility analysis and Ca2+ imaging during chemotaxis showed that CNG channel inhibition affected the changes in flagellar waveforms and Ca2+ efflux needed for the chemotactic turn. These results suggest that CNG channels in Ciona sperm play a vital role in regulating sperm motility and intracellular Ca2+ regulation during chemotaxis.\n
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\n\n \n \n Shibata, D., Morita, M., Sato, Y., Shiba, K., Kitanobo, S., Yokoya, R., & Inaba, K.\n\n\n \n \n \n \n \n Axonemal growth and alignment during paraspermatogenesis in the marine gastropod Strombus luhuanus.\n \n \n \n \n\n\n \n\n\n\n
Frontiers in Cell and Developmental Biology, 10: 905748. June 2022.\n
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@article{shibata_axonemal_2022,\n\ttitle = {Axonemal growth and alignment during paraspermatogenesis in the marine gastropod \\textit{{Strombus} luhuanus}},\n\tvolume = {10},\n\tissn = {2296-634X},\n\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2022.905748/full},\n\tdoi = {10.3389/fcell.2022.905748},\n\tabstract = {Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail\n S. luhuanus\n . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.},\n\turldate = {2022-07-12},\n\tjournal = {Frontiers in Cell and Developmental Biology},\n\tauthor = {Shibata, Daisuke and Morita, Masaya and Sato, Yu and Shiba, Kogiku and Kitanobo, Seiya and Yokoya, Ryo and Inaba, Kazuo},\n\tmonth = jun,\n\tyear = {2022},\n\tpages = {905748},\n}\n\n\n
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\n Parasperm are non-fertilizing sperm that are produced simultaneously with fertile eusperm. They occur in several animal species and show considerable morphological diversity. We investigated the dynamics of axonemes during paraspermatogenesis in the marine snail S. luhuanus . Mature parasperm were characterized by two lateral undulating membranes for motility and many globular vesicles. Axonemes were first observed as brush-like structures that extruded from the anterior region. Multiple axonemes longer than the brush then started to extend inside the cytoplasm towards the posterior region. The mass of the axonemes separated into two lateral rows and formed an undulating membrane that drives bidirectional swimming in the mature parasperm. The central pair of axonemes was aligned in the undulating membrane, resulting in cooperative bend propagation. During paraspermatogenesis, centrioles were largely diminished and localized to the anterior region. CEP290, a major component of the transition zone, showed a broad distribution in the anterior area. Axonemes in the posterior region showed a 9 + 0 structure with both outer and inner arm dyneins. These observations provide a structural basis for understanding the physiological functions of parasperm in marine reproductive strategies.\n
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\n\n \n \n Sugiura, K., Shiba, K., Inaba, K., & Matsumoto, M.\n\n\n \n \n \n \n \n Morphological differences in tardigrade spermatozoa induce variation in gamete motility.\n \n \n \n \n\n\n \n\n\n\n
BMC Zoology, 7(1): 8. December 2022.\n
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@article{sugiura_morphological_2022,\n\ttitle = {Morphological differences in tardigrade spermatozoa induce variation in gamete motility},\n\tvolume = {7},\n\tissn = {2056-3132},\n\turl = {https:///bmczool.biomedcentral.com/articles/10.1186/s40850-022-00109-w},\n\tdoi = {10.1186/s40850-022-00109-w},\n\tabstract = {Abstract\n \n Background\n \n Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades,\n Paramacrobiotus\n sp. and\n Macrobiotus shonaicus,\n which have longer and shorter spermatozoa, respectively.\n \n \n \n Results\n \n The movement of spermatozoa was faster in\n Paramacrobiotus\n sp. than in\n M. shonaicus\n , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of\n Paramacrobiotus\n sp. spermatozoa swung harder during turning; in contrast, the tail of\n M. shonaicus\n moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species.\n \n \n \n Conclusions\n \n This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of\n Paramacrobiotus\n sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2022-02-07},\n\tjournal = {BMC Zoology},\n\tauthor = {Sugiura, Kenta and Shiba, Kogiku and Inaba, Kazuo and Matsumoto, Midori},\n\tmonth = dec,\n\tyear = {2022},\n\tpages = {8},\n}\n\n\n
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\n Abstract Background Fertilization is an event at the beginning of ontogeny. Successful fertilization depends on strategies for uniting female and male gametes that developed throughout evolutionary history. In some species of tardigrades, investigations of reproduction have revealed that released spermatozoa swim in the water to reach a female, after which the gametes are stored in her body. The morphology of the spermatozoa includes a coiled nucleus and a species-specific-length acrosome. Although the mating behaviour and morphology of tardigrades have been reported, the motility of male gametes remains unknown. Here, using a high-speed camera, we recorded the spermatozoon motilities of two tardigrades, Paramacrobiotus sp. and Macrobiotus shonaicus, which have longer and shorter spermatozoa, respectively. Results The movement of spermatozoa was faster in Paramacrobiotus sp. than in M. shonaicus , but the beat frequencies of the tails were equal, suggesting that the long tail improved acceleration. In both species, the head part consisting of a coiled nucleus and an acrosome did not swing, in contrast to the tail. The head part of Paramacrobiotus sp. spermatozoa swung harder during turning; in contrast, the tail of M. shonaicus moved more widely than the head. Finally, after mating, the spermatozoa that reached the female aggregated around the cloaca while waiting to enter her body in both tested species. Conclusions This study provides results for the first observations and analyses of individual spermatozoon motility in tardigrades. A comparison of the spermatozoon movements of the two tardigrades suggested that the motilities of the male gametes were affected by morphological differences, where the longer spermatozoa swam faster and the shorter ones showed more stable swimming. Swimming was mainly induced by tail movement, but the long head of Paramacrobiotus sp. spermatozoa might be especially important for turning. In addition, observations of mated female cloacae suggested that the head parts of the spermatozoa were required for aggregation around the cloaca of a mated female.\n
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\n\n \n \n Suzuki, H., & Yaguchi, S.\n\n\n \n \n \n \n \n Direct TGF ‐ß signaling via alk4/5/7 pathway is involved in gut bending in sea urchin embryos.\n \n \n \n \n\n\n \n\n\n\n
Developmental Dynamics, 251(1): 226–234. January 2022.\n
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@article{suzuki_direct_2022,\n\ttitle = {Direct {TGF} ‐ß signaling via alk4/5/7 pathway is involved in gut bending in sea urchin embryos},\n\tvolume = {251},\n\tissn = {1058-8388, 1097-0177},\n\tshorttitle = {Direct {\\textless}span style="font-variant},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvdy.442},\n\tdoi = {10.1002/dvdy.442},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2022-01-25},\n\tjournal = {Developmental Dynamics},\n\tauthor = {Suzuki, Haruka and Yaguchi, Shunsuke},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {226--234},\n}\n\n\n
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\n\n \n \n Wada, S., Satoh, Y., & Hama, T.\n\n\n \n \n \n \n \n Massive loss and microbial decomposition in reproductive biomass of Zostera marina.\n \n \n \n \n\n\n \n\n\n\n
Estuarine, Coastal and Shelf Science, 275: 107986. September 2022.\n
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@article{wada_massive_2022,\n\ttitle = {Massive loss and microbial decomposition in reproductive biomass of {Zostera} marina},\n\tvolume = {275},\n\tissn = {02727714},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S027277142200244X},\n\tdoi = {10.1016/j.ecss.2022.107986},\n\tlanguage = {en},\n\turldate = {2022-09-01},\n\tjournal = {Estuarine, Coastal and Shelf Science},\n\tauthor = {Wada, Shigeki and Satoh, Yuhi and Hama, Takeo},\n\tmonth = sep,\n\tyear = {2022},\n\tpages = {107986},\n}\n\n\n
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\n\n \n \n Yaguchi, S., Taniguchi, Y., Suzuki, H., Kamata, M., & Yaguchi, J.\n\n\n \n \n \n \n \n Planktonic sea urchin larvae change their swimming direction in response to strong photoirradiation.\n \n \n \n \n\n\n \n\n\n\n
PLOS Genetics, 18(2): e1010033. February 2022.\n
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@article{yaguchi_planktonic_2022,\n\ttitle = {Planktonic sea urchin larvae change their swimming direction in response to strong photoirradiation},\n\tvolume = {18},\n\tissn = {1553-7404},\n\turl = {https://dx.plos.org/10.1371/journal.pgen.1010033},\n\tdoi = {10.1371/journal.pgen.1010033},\n\tabstract = {To survive, organisms need to precisely respond to various environmental factors, such as light and gravity. Among these, light is so important for most life on Earth that light-response systems have become extraordinarily developed during evolution, especially in multicellular animals. A combination of photoreceptors, nervous system components, and effectors allows these animals to respond to light stimuli. In most macroscopic animals, muscles function as effectors responding to light, and in some microscopic aquatic animals, cilia play a role. It is likely that the cilia-based response was the first to develop and that it has been substituted by the muscle-based response along with increases in body size. However, although the function of muscle appears prominent, it is poorly understood whether ciliary responses to light are present and/or functional, especially in deuterostomes, because it is possible that these responses are too subtle to be observed, unlike muscle responses. Here, we show that planktonic sea urchin larvae reverse their swimming direction due to the inhibitory effect of light on the cholinergic neuron signaling{\\textgreater}forward swimming pathway. We found that strong photoirradiation of larvae that stay on the surface of seawater immediately drives the larvae away from the surface due to backward swimming. When Opsin2, which is expressed in mesenchymal cells in larval arms, is knocked down, the larvae do not show backward swimming under photoirradiation. Although Opsin2-expressing cells are not neuronal cells, immunohistochemical analysis revealed that they directly attach to cholinergic neurons, which are thought to regulate forward swimming. These data indicate that light, through Opsin2, inhibits the activity of cholinergic signaling, which normally promotes larval forward swimming, and that the light-dependent ciliary response is present in deuterostomes. These findings shed light on how light-responsive tissues/organelles have been conserved and diversified during evolution.},\n\tlanguage = {en},\n\tnumber = {2},\n\turldate = {2024-04-04},\n\tjournal = {PLOS Genetics},\n\tauthor = {Yaguchi, Shunsuke and Taniguchi, Yuri and Suzuki, Haruka and Kamata, Mai and Yaguchi, Junko},\n\teditor = {Desplan, Claude},\n\tmonth = feb,\n\tyear = {2022},\n\tpages = {e1010033},\n}\n\n\n
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\n To survive, organisms need to precisely respond to various environmental factors, such as light and gravity. Among these, light is so important for most life on Earth that light-response systems have become extraordinarily developed during evolution, especially in multicellular animals. A combination of photoreceptors, nervous system components, and effectors allows these animals to respond to light stimuli. In most macroscopic animals, muscles function as effectors responding to light, and in some microscopic aquatic animals, cilia play a role. It is likely that the cilia-based response was the first to develop and that it has been substituted by the muscle-based response along with increases in body size. However, although the function of muscle appears prominent, it is poorly understood whether ciliary responses to light are present and/or functional, especially in deuterostomes, because it is possible that these responses are too subtle to be observed, unlike muscle responses. Here, we show that planktonic sea urchin larvae reverse their swimming direction due to the inhibitory effect of light on the cholinergic neuron signaling\\textgreaterforward swimming pathway. We found that strong photoirradiation of larvae that stay on the surface of seawater immediately drives the larvae away from the surface due to backward swimming. When Opsin2, which is expressed in mesenchymal cells in larval arms, is knocked down, the larvae do not show backward swimming under photoirradiation. Although Opsin2-expressing cells are not neuronal cells, immunohistochemical analysis revealed that they directly attach to cholinergic neurons, which are thought to regulate forward swimming. These data indicate that light, through Opsin2, inhibits the activity of cholinergic signaling, which normally promotes larval forward swimming, and that the light-dependent ciliary response is present in deuterostomes. These findings shed light on how light-responsive tissues/organelles have been conserved and diversified during evolution.\n
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\n\n \n \n Yaguchi, S., & Wada, H.\n\n\n \n \n \n \n \n Marine genomics, transcriptomics, and beyond in developmental, cell, and evolutionary biology.\n \n \n \n \n\n\n \n\n\n\n
Development, Growth & Differentiation, 64(4): 196–197. May 2022.\n
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@article{yaguchi_marine_2022,\n\ttitle = {Marine genomics, transcriptomics, and beyond in developmental, cell, and evolutionary biology},\n\tvolume = {64},\n\tissn = {0012-1592, 1440-169X},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12792},\n\tdoi = {10.1111/dgd.12792},\n\tlanguage = {en},\n\tnumber = {4},\n\turldate = {2024-04-04},\n\tjournal = {Development, Growth \\& Differentiation},\n\tauthor = {Yaguchi, Shunsuke and Wada, Hiroshi},\n\tmonth = may,\n\tyear = {2022},\n\tpages = {196--197},\n}\n\n\n
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\n\n \n \n Yaguchi, S., & Yaguchi, J.\n\n\n \n \n \n \n \n Temnopleurus reevesii as a new sea urchin model in genetics.\n \n \n \n \n\n\n \n\n\n\n
Development, Growth & Differentiation,dgd.12768. January 2022.\n
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@article{yaguchi_temnopleurus_2022,\n\ttitle = {\\textit{{Temnopleurus} reevesii} as a new sea urchin model in genetics},\n\tissn = {0012-1592, 1440-169X},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12768},\n\tdoi = {10.1111/dgd.12768},\n\tlanguage = {en},\n\turldate = {2022-01-25},\n\tjournal = {Development, Growth \\& Differentiation},\n\tauthor = {Yaguchi, Shunsuke and Yaguchi, Junko},\n\tmonth = jan,\n\tyear = {2022},\n\tpages = {dgd.12768},\n}\n\n\n
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\n\n \n \n Yamagishi, M., Huang, T., Hozumi, A., Onuma, T. A., Sasakura, Y., & Ogasawara, M.\n\n\n \n \n \n \n \n Differentiation of endostyle cells by Nkx2-1 and FoxE in the ascidian Ciona intestinalis type A: insights into shared gene regulation in glandular- and thyroid-equivalent elements of the chordate endostyle.\n \n \n \n \n\n\n \n\n\n\n
Cell and Tissue Research. September 2022.\n
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@article{yamagishi_differentiation_2022,\n\ttitle = {Differentiation of endostyle cells by {Nkx2}-1 and {FoxE} in the ascidian \\textit{{Ciona} intestinalis} type {A}: insights into shared gene regulation in glandular- and thyroid-equivalent elements of the chordate endostyle},\n\tissn = {0302-766X, 1432-0878},\n\tshorttitle = {Differentiation of endostyle cells by {Nkx2}-1 and {FoxE} in the ascidian {Ciona} intestinalis type {A}},\n\turl = {https://link.springer.com/10.1007/s00441-022-03679-w},\n\tdoi = {10.1007/s00441-022-03679-w},\n\tlanguage = {en},\n\turldate = {2022-11-01},\n\tjournal = {Cell and Tissue Research},\n\tauthor = {Yamagishi, Masayuki and Huang, Taoruo and Hozumi, Akiko and Onuma, Takeshi A. and Sasakura, Yasunori and Ogasawara, Michio},\n\tmonth = sep,\n\tyear = {2022},\n}\n\n\n
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