var bibbase_data = {"data":"<img src=\"//bibbase.org/img/ajax-loader.gif\" id=\"spinner\"\n  style=\"display: none;\" alt=\"Loading..\" />\n\n<div id=\"bibbase\">\n\n  <script type=\"text/javascript\">\n    var bibbase = {\n      params: {\"bib\":\"https://api.zotero.org/users/3476755/collections/NJGLI74A/items?key=ckhubDoO51X1cQZZ35NkGdxk&format=bibtex&limit=100\",\"jsonp\":\"1\",\"authorFirst\":\"1\",\"hidemenu\":\"true\",\"commas\":\"true\",\"sort\":\"author_short\",\"groupby\":\"\",\"host\":\"bibbase.org\"},\n      data: [{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve\",\"volume\":\"147\",\"issn\":\"1470-1626, 1741-7899\",\"url\":\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\",\"doi\":\"10.1530/REP-13-0418\",\"abstract\":\"Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam ( Ruditapes philippinarum ), Pacific oyster ( Crassostrea gigas ), and Japanese scallop ( Patinopecten yessoensis ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K + /H + exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K + concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K + or ≥2.5 mM 4-aminopyridine, suggesting K + efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca 2 + is required for Ca 2 + /calmodulin-dependent flagellar beating. Ca 2 + influx occurs via Ca 2 + channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca 2 + channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca 2 + rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca 2 + channel blocker. Extracellular Na + is also essential for sperm motility initiation via regulation of Na + /Ca 2 + exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K + , Ca 2 + , and Na + .\",\"number\":\"3\",\"urldate\":\"2021-07-27\",\"journal\":\"REPRODUCTION\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Alavi\"],\"firstnames\":[\"Sayyed\",\"Mohammad\",\"Hadi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Matsumura\"],\"firstnames\":[\"Natsuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Itoh\"],\"firstnames\":[\"Naoki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Takahashi\"],\"firstnames\":[\"Keisuke\",\"G\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Osada\"],\"firstnames\":[\"Makoto\"],\"suffixes\":[]}],\"month\":\"March\",\"year\":\"2014\",\"pages\":\"331–345\",\"bibtex\":\"@article{alavi_roles_2014,\\n\\ttitle = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},\\n\\tvolume = {147},\\n\\tissn = {1470-1626, 1741-7899},\\n\\turl = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},\\n\\tdoi = {10.1530/REP-13-0418},\\n\\tabstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (\\n              Ruditapes\\n              philippinarum\\n              ), Pacific oyster (\\n              Crassostrea gigas\\n              ), and Japanese scallop (\\n              Patinopecten yessoensis\\n              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K\\n              +\\n              /H\\n              +\\n              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K\\n              +\\n              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K\\n              +\\n              or ≥2.5 mM 4-aminopyridine, suggesting K\\n              +\\n              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca\\n              2\\n              +\\n              is required for Ca\\n              2\\n              +\\n              /calmodulin-dependent flagellar beating. Ca\\n              2\\n              +\\n              influx occurs via Ca\\n              2\\n              +\\n              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca\\n              2\\n              +\\n              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca\\n              2\\n              +\\n              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca\\n              2\\n              +\\n              channel blocker. Extracellular Na\\n              +\\n              is also essential for sperm motility initiation via regulation of Na\\n              +\\n              /Ca\\n              2\\n              +\\n              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K\\n              +\\n              , Ca\\n              2\\n              +\\n              , and Na\\n              +\\n              .},\\n\\tnumber = {3},\\n\\turldate = {2021-07-27},\\n\\tjournal = {REPRODUCTION},\\n\\tauthor = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},\\n\\tmonth = mar,\\n\\tyear = {2014},\\n\\tpages = {331--345},\\n}\\n\\n\",\"author_short\":[\"Alavi, S. M. H.\",\"Matsumura, N.\",\"Shiba, K.\",\"Itoh, N.\",\"Takahashi, K. G\",\"Inaba, K.\",\"Osada, M.\"],\"key\":\"alavi_roles_2014\",\"id\":\"alavi_roles_2014\",\"bibbaseid\":\"alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity\",\"volume\":\"40\",\"issn\":\"0920-1742, 1573-5168\",\"url\":\"http://link.springer.com/10.1007/s10695-014-9933-8\",\"doi\":\"10.1007/s10695-014-9933-8\",\"language\":\"en\",\"number\":\"5\",\"urldate\":\"2021-07-27\",\"journal\":\"Fish Physiology and Biochemistry\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Alavi\"],\"firstnames\":[\"Sayyed\",\"Mohammad\",\"Hadi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Postlerová-Maňásková\"],\"firstnames\":[\"Pavla\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hatef\"],\"firstnames\":[\"Azadeh\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pšenička\"],\"firstnames\":[\"Martin\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pěknicová\"],\"firstnames\":[\"Jana\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ciereszko\"],\"firstnames\":[\"Andrzej\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Linhart\"],\"firstnames\":[\"Otomar\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2014\",\"pages\":\"1393–1398\",\"bibtex\":\"@article{alavi_protease_2014,\\n\\ttitle = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},\\n\\tvolume = {40},\\n\\tissn = {0920-1742, 1573-5168},\\n\\turl = {http://link.springer.com/10.1007/s10695-014-9933-8},\\n\\tdoi = {10.1007/s10695-014-9933-8},\\n\\tlanguage = {en},\\n\\tnumber = {5},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Fish Physiology and Biochemistry},\\n\\tauthor = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},\\n\\tmonth = oct,\\n\\tyear = {2014},\\n\\tpages = {1393--1398},\\n}\\n\\n\",\"author_short\":[\"Alavi, S. M. H.\",\"Postlerová-Maňásková, P.\",\"Hatef, A.\",\"Pšenička, M.\",\"Pěknicová, J.\",\"Inaba, K.\",\"Ciereszko, A.\",\"Linhart, O.\"],\"key\":\"alavi_protease_2014\",\"id\":\"alavi_protease_2014\",\"bibbaseid\":\"alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/s10695-014-9933-8\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Biotic Control of Skeletal Growth by Scleractinian Corals in Aragonite–Calcite Seas\",\"volume\":\"9\",\"issn\":\"1932-6203\",\"url\":\"https://dx.plos.org/10.1371/journal.pone.0091021\",\"doi\":\"10.1371/journal.pone.0091021\",\"language\":\"en\",\"number\":\"3\",\"urldate\":\"2021-07-27\",\"journal\":\"PLoS ONE\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Higuchi\"],\"firstnames\":[\"Tomihiko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Fujimura\"],\"firstnames\":[\"Hiroyuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yuyama\"],\"firstnames\":[\"Ikuko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Harii\"],\"firstnames\":[\"Saki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Agostini\"],\"firstnames\":[\"Sylvain\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Oomori\"],\"firstnames\":[\"Tamotsu\"],\"suffixes\":[]}],\"editor\":[{\"propositions\":[],\"lastnames\":[\"Roberts\"],\"firstnames\":[\"John\",\"Murray\"],\"suffixes\":[]}],\"month\":\"March\",\"year\":\"2014\",\"pages\":\"e91021\",\"bibtex\":\"@article{higuchi_biotic_2014,\\n\\ttitle = {Biotic {Control} of {Skeletal} {Growth} by {Scleractinian} {Corals} in {Aragonite}–{Calcite} {Seas}},\\n\\tvolume = {9},\\n\\tissn = {1932-6203},\\n\\turl = {https://dx.plos.org/10.1371/journal.pone.0091021},\\n\\tdoi = {10.1371/journal.pone.0091021},\\n\\tlanguage = {en},\\n\\tnumber = {3},\\n\\turldate = {2021-07-27},\\n\\tjournal = {PLoS ONE},\\n\\tauthor = {Higuchi, Tomihiko and Fujimura, Hiroyuki and Yuyama, Ikuko and Harii, Saki and Agostini, Sylvain and Oomori, Tamotsu},\\n\\teditor = {Roberts, John Murray},\\n\\tmonth = mar,\\n\\tyear = {2014},\\n\\tpages = {e91021},\\n}\\n\\n\",\"author_short\":[\"Higuchi, T.\",\"Fujimura, H.\",\"Yuyama, I.\",\"Harii, S.\",\"Agostini, S.\",\"Oomori, T.\"],\"editor_short\":[\"Roberts, J. M.\"],\"key\":\"higuchi_biotic_2014\",\"id\":\"higuchi_biotic_2014\",\"bibbaseid\":\"higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://dx.plos.org/10.1371/journal.pone.0091021\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":7,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (<i>Coturnix japonica</i>)\",\"volume\":\"147\",\"issn\":\"1470-1626, 1741-7899\",\"url\":\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\",\"doi\":\"10.1530/REP-13-0439\",\"abstract\":\"Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.\",\"number\":\"2\",\"urldate\":\"2021-07-27\",\"journal\":\"REPRODUCTION\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Hiyama\"],\"firstnames\":[\"Gen\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Matsuzaki\"],\"firstnames\":[\"Mei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mizushima\"],\"firstnames\":[\"Shusei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dohra\"],\"firstnames\":[\"Hideo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ikegami\"],\"firstnames\":[\"Keisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshimura\"],\"firstnames\":[\"Takashi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasanami\"],\"firstnames\":[\"Tomohiro\"],\"suffixes\":[]}],\"month\":\"February\",\"year\":\"2014\",\"pages\":\"167–178\",\"bibtex\":\"@article{hiyama_sperm_2014,\\n\\ttitle = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\\\\textit{{Coturnix} japonica})},\\n\\tvolume = {147},\\n\\tissn = {1470-1626, 1741-7899},\\n\\turl = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},\\n\\tdoi = {10.1530/REP-13-0439},\\n\\tabstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility\\n              in vitro\\n              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of\\n              HSP70\\n              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific\\n              in situ\\n              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization\\n              in vivo\\n              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},\\n\\tnumber = {2},\\n\\turldate = {2021-07-27},\\n\\tjournal = {REPRODUCTION},\\n\\tauthor = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},\\n\\tmonth = feb,\\n\\tyear = {2014},\\n\\tpages = {167--178},\\n}\\n\\n\",\"author_short\":[\"Hiyama, G.\",\"Matsuzaki, M.\",\"Mizushima, S.\",\"Dohra, H.\",\"Ikegami, K.\",\"Yoshimura, T.\",\"Shiba, K.\",\"Inaba, K.\",\"Sasanami, T.\"],\"key\":\"hiyama_sperm_2014\",\"id\":\"hiyama_sperm_2014\",\"bibbaseid\":\"hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"incollection\",\"type\":\"incollection\",\"address\":\"Tokyo\",\"title\":\"Structure, Function, and Phylogenetic Consideration of Calaxin\",\"isbn\":\"978-4-431-54588-0 978-4-431-54589-7\",\"url\":\"http://link.springer.com/10.1007/978-4-431-54589-7_5\",\"language\":\"en\",\"urldate\":\"2021-07-27\",\"booktitle\":\"Sexual Reproduction in Animals and Plants\",\"publisher\":\"Springer Japan\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mizuno\"],\"firstnames\":[\"Katsutoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]}],\"editor\":[{\"propositions\":[],\"lastnames\":[\"Sawada\"],\"firstnames\":[\"Hitoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inoue\"],\"firstnames\":[\"Naokazu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Iwano\"],\"firstnames\":[\"Megumi\"],\"suffixes\":[]}],\"year\":\"2014\",\"doi\":\"10.1007/978-4-431-54589-7_5\",\"pages\":\"49–57\",\"bibtex\":\"@incollection{sawada_structure_2014,\\n\\taddress = {Tokyo},\\n\\ttitle = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},\\n\\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\\n\\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_5},\\n\\tlanguage = {en},\\n\\turldate = {2021-07-27},\\n\\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\\n\\tpublisher = {Springer Japan},\\n\\tauthor = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},\\n\\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\\n\\tyear = {2014},\\n\\tdoi = {10.1007/978-4-431-54589-7_5},\\n\\tpages = {49--57},\\n}\\n\\n\",\"author_short\":[\"Inaba, K.\",\"Mizuno, K.\",\"Shiba, K.\"],\"editor_short\":[\"Sawada, H.\",\"Inoue, N.\",\"Iwano, M.\"],\"key\":\"sawada_structure_2014\",\"id\":\"sawada_structure_2014\",\"bibbaseid\":\"inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: FUNCTIONS OF GNRH IN ASCIDIANS\",\"volume\":\"243\",\"issn\":\"10588388\",\"shorttitle\":\"Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176\",\"doi\":\"10.1002/dvdy.24176\",\"language\":\"en\",\"number\":\"12\",\"urldate\":\"2021-07-27\",\"journal\":\"Developmental Dynamics\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kamiya\"],\"firstnames\":[\"Chisato\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ohta\"],\"firstnames\":[\"Naoyuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ogura\"],\"firstnames\":[\"Yosuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshida\"],\"firstnames\":[\"Keita\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Horie\"],\"firstnames\":[\"Takeo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kusakabe\"],\"firstnames\":[\"Takehiro\",\"G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Satake\"],\"firstnames\":[\"Honoo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasakura\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]}],\"month\":\"December\",\"year\":\"2014\",\"pages\":\"1524–1535\",\"bibtex\":\"@article{kamiya_nonreproductive_2014,\\n\\ttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: {FUNCTIONS} {OF} {GNRH} {IN} {ASCIDIANS}},\\n\\tvolume = {243},\\n\\tissn = {10588388},\\n\\tshorttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176},\\n\\tdoi = {10.1002/dvdy.24176},\\n\\tlanguage = {en},\\n\\tnumber = {12},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Developmental Dynamics},\\n\\tauthor = {Kamiya, Chisato and Ohta, Naoyuki and Ogura, Yosuke and Yoshida, Keita and Horie, Takeo and Kusakabe, Takehiro G. and Satake, Honoo and Sasakura, Yasunori},\\n\\tmonth = dec,\\n\\tyear = {2014},\\n\\tpages = {1524--1535},\\n}\\n\",\"author_short\":[\"Kamiya, C.\",\"Ohta, N.\",\"Ogura, Y.\",\"Yoshida, K.\",\"Horie, T.\",\"Kusakabe, T. G.\",\"Satake, H.\",\"Sasakura, Y.\"],\"key\":\"kamiya_nonreproductive_2014\",\"id\":\"kamiya_nonreproductive_2014\",\"bibbaseid\":\"kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"incollection\",\"type\":\"incollection\",\"address\":\"Totowa, NJ\",\"title\":\"Imaging Neural Development in Embryonic and Larval Sea Urchins\",\"volume\":\"1128\",\"isbn\":\"978-1-62703-973-4 978-1-62703-974-1\",\"url\":\"http://link.springer.com/10.1007/978-1-62703-974-1_9\",\"urldate\":\"2021-07-27\",\"booktitle\":\"Developmental Biology of the Sea Urchin and Other Marine Invertebrates\",\"publisher\":\"Humana Press\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Krupke\"],\"firstnames\":[\"Oliver\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yaguchi\"],\"firstnames\":[\"Shunsuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yaguchi\"],\"firstnames\":[\"Junko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burke\"],\"firstnames\":[\"Robert\",\"D.\"],\"suffixes\":[]}],\"editor\":[{\"propositions\":[],\"lastnames\":[\"Carroll\"],\"firstnames\":[\"David\",\"J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stricker\"],\"firstnames\":[\"Stephen\",\"A.\"],\"suffixes\":[]}],\"year\":\"2014\",\"doi\":\"10.1007/978-1-62703-974-1_9\",\"note\":\"Series Title: Methods in Molecular Biology\",\"pages\":\"147–160\",\"bibtex\":\"@incollection{carroll_imaging_2014,\\n\\taddress = {Totowa, NJ},\\n\\ttitle = {Imaging {Neural} {Development} in {Embryonic} and {Larval} {Sea} {Urchins}},\\n\\tvolume = {1128},\\n\\tisbn = {978-1-62703-973-4 978-1-62703-974-1},\\n\\turl = {http://link.springer.com/10.1007/978-1-62703-974-1_9},\\n\\turldate = {2021-07-27},\\n\\tbooktitle = {Developmental {Biology} of the {Sea} {Urchin} and {Other} {Marine} {Invertebrates}},\\n\\tpublisher = {Humana Press},\\n\\tauthor = {Krupke, Oliver and Yaguchi, Shunsuke and Yaguchi, Junko and Burke, Robert D.},\\n\\teditor = {Carroll, David J. and Stricker, Stephen A.},\\n\\tyear = {2014},\\n\\tdoi = {10.1007/978-1-62703-974-1_9},\\n\\tnote = {Series Title: Methods in Molecular Biology},\\n\\tpages = {147--160},\\n}\\n\\n\",\"author_short\":[\"Krupke, O.\",\"Yaguchi, S.\",\"Yaguchi, J.\",\"Burke, R. D.\"],\"editor_short\":[\"Carroll, D. J.\",\"Stricker, S. A.\"],\"key\":\"carroll_imaging_2014\",\"id\":\"carroll_imaging_2014\",\"bibbaseid\":\"krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/978-1-62703-974-1_9\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"The birth of quail chicks after intracytoplasmic sperm injection\",\"volume\":\"141\",\"issn\":\"1477-9129, 0950-1991\",\"url\":\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\",\"doi\":\"10.1242/dev.111765\",\"abstract\":\"Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.\",\"language\":\"en\",\"number\":\"19\",\"urldate\":\"2021-07-27\",\"journal\":\"Development\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Mizushima\"],\"firstnames\":[\"Shusei\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hiyama\"],\"firstnames\":[\"Gen\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dohra\"],\"firstnames\":[\"Hideo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ono\"],\"firstnames\":[\"Tamao\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shimada\"],\"firstnames\":[\"Kiyoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasanami\"],\"firstnames\":[\"Tomohiro\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2014\",\"pages\":\"3799–3806\",\"bibtex\":\"@article{mizushima_birth_2014,\\n\\ttitle = {The birth of quail chicks after intracytoplasmic sperm injection},\\n\\tvolume = {141},\\n\\tissn = {1477-9129, 0950-1991},\\n\\turl = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},\\n\\tdoi = {10.1242/dev.111765},\\n\\tabstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},\\n\\tlanguage = {en},\\n\\tnumber = {19},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Development},\\n\\tauthor = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},\\n\\tmonth = oct,\\n\\tyear = {2014},\\n\\tpages = {3799--3806},\\n}\\n\\n\",\"author_short\":[\"Mizushima, S.\",\"Hiyama, G.\",\"Shiba, K.\",\"Inaba, K.\",\"Dohra, H.\",\"Ono, T.\",\"Shimada, K.\",\"Sasanami, T.\"],\"key\":\"mizushima_birth_2014\",\"id\":\"mizushima_birth_2014\",\"bibbaseid\":\"mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":4,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Survey of the Japanese Coast Reveals Abundant Placozoan Populations in the Northern Pacific Ocean\",\"volume\":\"4\",\"issn\":\"2045-2322\",\"url\":\"http://www.nature.com/articles/srep05356\",\"doi\":\"10.1038/srep05356\",\"language\":\"en\",\"number\":\"1\",\"urldate\":\"2021-07-27\",\"journal\":\"Scientific Reports\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Nakano\"],\"firstnames\":[\"Hiroaki\"],\"suffixes\":[]}],\"year\":\"2014\",\"pages\":\"5356\",\"bibtex\":\"@article{nakano_survey_2014,\\n\\ttitle = {Survey of the {Japanese} {Coast} {Reveals} {Abundant} {Placozoan} {Populations} in the {Northern} {Pacific} {Ocean}},\\n\\tvolume = {4},\\n\\tissn = {2045-2322},\\n\\turl = {http://www.nature.com/articles/srep05356},\\n\\tdoi = {10.1038/srep05356},\\n\\tlanguage = {en},\\n\\tnumber = {1},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Scientific Reports},\\n\\tauthor = {Nakano, Hiroaki},\\n\\tyear = {2014},\\n\\tpages = {5356},\\n}\\n\\n\",\"author_short\":[\"Nakano, H.\"],\"key\":\"nakano_survey_2014\",\"id\":\"nakano_survey_2014\",\"bibbaseid\":\"nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://www.nature.com/articles/srep05356\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"CRISPR/Cas9-mediated gene knockout in the ascidian <i>Ciona intestinalis</i>\",\"volume\":\"56\",\"issn\":\"0012-1592, 1440-169X\",\"shorttitle\":\"\\\\textlessspan style=\\\"font-variant\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149\",\"doi\":\"10.1111/dgd.12149\",\"language\":\"en\",\"number\":\"7\",\"urldate\":\"2021-07-27\",\"journal\":\"Development, Growth & Differentiation\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sasaki\"],\"firstnames\":[\"Haruka\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshida\"],\"firstnames\":[\"Keita\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hozumi\"],\"firstnames\":[\"Akiko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasakura\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]}],\"month\":\"September\",\"year\":\"2014\",\"pages\":\"499–510\",\"bibtex\":\"@article{sasaki_crisprcas9-mediated_2014,\\n\\ttitle = {{CRISPR}/{Cas9}-mediated gene knockout in the ascidian \\\\textit{{Ciona} intestinalis}},\\n\\tvolume = {56},\\n\\tissn = {0012-1592, 1440-169X},\\n\\tshorttitle = {{\\\\textless}span style=\\\"font-variant},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149},\\n\\tdoi = {10.1111/dgd.12149},\\n\\tlanguage = {en},\\n\\tnumber = {7},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Development, Growth \\\\& Differentiation},\\n\\tauthor = {Sasaki, Haruka and Yoshida, Keita and Hozumi, Akiko and Sasakura, Yasunori},\\n\\tmonth = sep,\\n\\tyear = {2014},\\n\\tpages = {499--510},\\n}\\n\\n\",\"author_short\":[\"Sasaki, H.\",\"Yoshida, K.\",\"Hozumi, A.\",\"Sasakura, Y.\"],\"key\":\"sasaki_crisprcas9-mediated_2014\",\"id\":\"sasaki_crisprcas9-mediated_2014\",\"bibbaseid\":\"sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"incollection\",\"type\":\"incollection\",\"address\":\"Tokyo\",\"title\":\"Germline Transformation in the Ascidian <i>Ciona intestinalis</i>\",\"isbn\":\"978-4-431-54588-0 978-4-431-54589-7\",\"url\":\"http://link.springer.com/10.1007/978-4-431-54589-7_38\",\"language\":\"en\",\"urldate\":\"2021-07-27\",\"booktitle\":\"Sexual Reproduction in Animals and Plants\",\"publisher\":\"Springer Japan\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sasakura\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]}],\"editor\":[{\"propositions\":[],\"lastnames\":[\"Sawada\"],\"firstnames\":[\"Hitoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inoue\"],\"firstnames\":[\"Naokazu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Iwano\"],\"firstnames\":[\"Megumi\"],\"suffixes\":[]}],\"year\":\"2014\",\"doi\":\"10.1007/978-4-431-54589-7_38\",\"pages\":\"465–473\",\"bibtex\":\"@incollection{sawada_germline_2014,\\n\\taddress = {Tokyo},\\n\\ttitle = {Germline {Transformation} in the {Ascidian} \\\\textit{{Ciona} intestinalis}},\\n\\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\\n\\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_38},\\n\\tlanguage = {en},\\n\\turldate = {2021-07-27},\\n\\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\\n\\tpublisher = {Springer Japan},\\n\\tauthor = {Sasakura, Yasunori},\\n\\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\\n\\tyear = {2014},\\n\\tdoi = {10.1007/978-4-431-54589-7_38},\\n\\tpages = {465--473},\\n}\\n\\n\",\"author_short\":[\"Sasakura, Y.\"],\"editor_short\":[\"Sawada, H.\",\"Inoue, N.\",\"Iwano, M.\"],\"key\":\"sawada_germline_2014\",\"id\":\"sawada_germline_2014\",\"bibbaseid\":\"sasakura-germlinetransformationintheascidianicionaintestinalisi-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://link.springer.com/10.1007/978-4-431-54589-7_38\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in <i>Ciona</i> Sperm\",\"volume\":\"15\",\"issn\":\"1422-0067\",\"url\":\"http://www.mdpi.com/1422-0067/15/8/13192\",\"doi\":\"10.3390/ijms150813192\",\"language\":\"en\",\"number\":\"8\",\"urldate\":\"2021-07-27\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"July\",\"year\":\"2014\",\"pages\":\"13192–13208\",\"bibtex\":\"@article{shiba_distinct_2014,\\n\\ttitle = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \\\\textit{{Ciona}} {Sperm}},\\n\\tvolume = {15},\\n\\tissn = {1422-0067},\\n\\turl = {http://www.mdpi.com/1422-0067/15/8/13192},\\n\\tdoi = {10.3390/ijms150813192},\\n\\tlanguage = {en},\\n\\tnumber = {8},\\n\\turldate = {2021-07-27},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = jul,\\n\\tyear = {2014},\\n\\tpages = {13192--13208},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"shiba_distinct_2014\",\"id\":\"shiba_distinct_2014\",\"bibbaseid\":\"shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://www.mdpi.com/1422-0067/15/8/13192\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\\\textrm{2}}$\",\"volume\":\"137\",\"issn\":\"09247963\",\"url\":\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\",\"doi\":\"10.1016/j.jmarsys.2014.04.013\",\"language\":\"en\",\"urldate\":\"2021-07-27\",\"journal\":\"Journal of Marine Systems\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sung\"],\"firstnames\":[\"Chan-Gyung\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kim\"],\"firstnames\":[\"Tae\",\"Won\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Park\"],\"firstnames\":[\"Young-Gyu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kang\"],\"firstnames\":[\"Seong-Gil\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Choi\"],\"firstnames\":[\"Tae\",\"Seob\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Moon\"],\"firstnames\":[\"Seong-Dae\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Litvin\"],\"firstnames\":[\"Steve\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lee\"],\"firstnames\":[\"Kyu-Tae\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lee\"],\"firstnames\":[\"Jung-Suk\"],\"suffixes\":[]}],\"month\":\"September\",\"year\":\"2014\",\"pages\":\"67–73\",\"bibtex\":\"@article{sung_species_2014,\\n\\ttitle = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\\\\textrm{2}}$},\\n\\tvolume = {137},\\n\\tissn = {09247963},\\n\\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},\\n\\tdoi = {10.1016/j.jmarsys.2014.04.013},\\n\\tlanguage = {en},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Journal of Marine Systems},\\n\\tauthor = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},\\n\\tmonth = sep,\\n\\tyear = {2014},\\n\\tpages = {67--73},\\n}\\n\\n\",\"author_short\":[\"Sung, C.\",\"Kim, T. W.\",\"Park, Y.\",\"Kang, S.\",\"Inaba, K.\",\"Shiba, K.\",\"Choi, T. S.\",\"Moon, S.\",\"Litvin, S.\",\"Lee, K.\",\"Lee, J.\"],\"key\":\"sung_species_2014\",\"id\":\"sung_species_2014\",\"bibbaseid\":\"sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in <i>Ciona</i>\",\"volume\":\"141\",\"issn\":\"1477-9129, 0950-1991\",\"url\":\"https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by\",\"doi\":\"10.1242/dev.099572\",\"abstract\":\"Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.\",\"language\":\"en\",\"number\":\"2\",\"urldate\":\"2021-07-27\",\"journal\":\"Development\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Treen\"],\"firstnames\":[\"Nicholas\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshida\"],\"firstnames\":[\"Keita\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sakuma\"],\"firstnames\":[\"Tetsushi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasaki\"],\"firstnames\":[\"Haruka\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kawai\"],\"firstnames\":[\"Narudo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yamamoto\"],\"firstnames\":[\"Takashi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasakura\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]}],\"month\":\"January\",\"year\":\"2014\",\"pages\":\"481–487\",\"bibtex\":\"@article{treen_tissue-specific_2014,\\n\\ttitle = {Tissue-specific and ubiquitous gene knockouts by {TALEN} electroporation provide new approaches to investigating gene function in \\\\textit{{Ciona}}},\\n\\tvolume = {141},\\n\\tissn = {1477-9129, 0950-1991},\\n\\turl = {https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by},\\n\\tdoi = {10.1242/dev.099572},\\n\\tabstract = {Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.},\\n\\tlanguage = {en},\\n\\tnumber = {2},\\n\\turldate = {2021-07-27},\\n\\tjournal = {Development},\\n\\tauthor = {Treen, Nicholas and Yoshida, Keita and Sakuma, Tetsushi and Sasaki, Haruka and Kawai, Narudo and Yamamoto, Takashi and Sasakura, Yasunori},\\n\\tmonth = jan,\\n\\tyear = {2014},\\n\\tpages = {481--487},\\n}\\n\\n\",\"author_short\":[\"Treen, N.\",\"Yoshida, K.\",\"Sakuma, T.\",\"Sasaki, H.\",\"Kawai, N.\",\"Yamamoto, T.\",\"Sasakura, Y.\"],\"key\":\"treen_tissue-specific_2014\",\"id\":\"treen_tissue-specific_2014\",\"bibbaseid\":\"treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, <i>Cynops pyrrhogaster</i>\",\"volume\":\"15\",\"issn\":\"1422-0067\",\"url\":\"http://www.mdpi.com/1422-0067/15/9/15210\",\"doi\":\"10.3390/ijms150915210\",\"language\":\"en\",\"number\":\"9\",\"urldate\":\"2021-07-27\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Yokoe\"],\"firstnames\":[\"Misato\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sano\"],\"firstnames\":[\"Makoto\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shibata\"],\"firstnames\":[\"Honami\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shibata\"],\"firstnames\":[\"Daisuke\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Takayama-Watanabe\"],\"firstnames\":[\"Eriko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Watanabe\"],\"firstnames\":[\"Akihiko\"],\"suffixes\":[]}],\"month\":\"August\",\"year\":\"2014\",\"pages\":\"15210–15224\",\"bibtex\":\"@article{yokoe_sperm_2014,\\n\\ttitle = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \\\\textit{{Cynops} pyrrhogaster}},\\n\\tvolume = {15},\\n\\tissn = {1422-0067},\\n\\turl = {http://www.mdpi.com/1422-0067/15/9/15210},\\n\\tdoi = {10.3390/ijms150915210},\\n\\tlanguage = {en},\\n\\tnumber = {9},\\n\\turldate = {2021-07-27},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},\\n\\tmonth = aug,\\n\\tyear = {2014},\\n\\tpages = {15210--15224},\\n}\\n\\n\",\"author_short\":[\"Yokoe, M.\",\"Sano, M.\",\"Shibata, H.\",\"Shibata, D.\",\"Takayama-Watanabe, E.\",\"Inaba, K.\",\"Watanabe, A.\"],\"key\":\"yokoe_sperm_2014\",\"id\":\"yokoe_sperm_2014\",\"bibbaseid\":\"yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"http://www.mdpi.com/1422-0067/15/9/15210\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Germ cell mutations of the ascidian <i>Ciona intestinalis</i> with TALE nucleases: germ cell mutagenesis in <i>ciona</i>\",\"volume\":\"52\",\"issn\":\"1526954X\",\"shorttitle\":\"Germ cell mutations of the ascidian <i>Ciona intestinalis</i> with TALE nucleases\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770\",\"doi\":\"10.1002/dvg.22770\",\"language\":\"en\",\"number\":\"5\",\"urldate\":\"2021-07-27\",\"journal\":\"genesis\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Yoshida\"],\"firstnames\":[\"Keita\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Treen\"],\"firstnames\":[\"Nicholas\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hozumi\"],\"firstnames\":[\"Akiko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sakuma\"],\"firstnames\":[\"Tetsushi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yamamoto\"],\"firstnames\":[\"Takashi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sasakura\"],\"firstnames\":[\"Yasunori\"],\"suffixes\":[]}],\"month\":\"May\",\"year\":\"2014\",\"pages\":\"431–439\",\"bibtex\":\"@article{yoshida_germ_2014,\\n\\ttitle = {Germ cell mutations of the ascidian \\\\textit{{Ciona} intestinalis} with {TALE} nucleases: germ cell mutagenesis in \\\\textit{ciona}},\\n\\tvolume = {52},\\n\\tissn = {1526954X},\\n\\tshorttitle = {Germ cell mutations of the ascidian \\\\textit{{Ciona} intestinalis} with {TALE} nucleases},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770},\\n\\tdoi = {10.1002/dvg.22770},\\n\\tlanguage = {en},\\n\\tnumber = {5},\\n\\turldate = {2021-07-27},\\n\\tjournal = {genesis},\\n\\tauthor = {Yoshida, Keita and Treen, Nicholas and Hozumi, Akiko and Sakuma, Tetsushi and Yamamoto, Takashi and Sasakura, Yasunori},\\n\\tmonth = may,\\n\\tyear = {2014},\\n\\tpages = {431--439},\\n}\\n\\n\",\"author_short\":[\"Yoshida, K.\",\"Treen, N.\",\"Hozumi, A.\",\"Sakuma, T.\",\"Yamamoto, T.\",\"Sasakura, Y.\"],\"key\":\"yoshida_germ_2014\",\"id\":\"yoshida_germ_2014\",\"bibbaseid\":\"yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階\",\"volume\":\"80\",\"issn\":\"0021-5392\",\"url\":\"https://ci.nii.ac.jp/naid/130004694420\",\"abstract\":\"1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 今 孝悦 , KHRUENIAM UDOM , 有元 貴文 , 吉川 尚 , 岡本 侑樹 , 石川 智士 日本水産学会誌 80(5), 837-837, 2014\",\"language\":\"en\",\"number\":\"5\",\"urldate\":\"2021-07-27\",\"journal\":\"日本水産学会誌\",\"author\":[{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"今孝悦\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Khrueniam\"],\"firstnames\":[\"Udom\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"有元貴文\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"吉川尚\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"岡本侑樹\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"石川智士\"],\"suffixes\":[]}],\"year\":\"2014\",\"note\":\"Publisher: 公益社団法人 日本水産学会\",\"pages\":\"837–837\",\"bibtex\":\"@article{_1_2014,\\n\\ttitle = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階},\\n\\tvolume = {80},\\n\\tissn = {0021-5392},\\n\\turl = {https://ci.nii.ac.jp/naid/130004694420},\\n\\tabstract = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 今 孝悦 , KHRUENIAM UDOM , 有元 貴文 , 吉川 尚 , 岡本 侑樹 , 石川 智士 日本水産学会誌 80(5), 837-837, 2014},\\n\\tlanguage = {en},\\n\\tnumber = {5},\\n\\turldate = {2021-07-27},\\n\\tjournal = {日本水産学会誌},\\n\\tauthor = {{今孝悦} and Khrueniam, Udom and {有元貴文} and {吉川尚} and {岡本侑樹} and {石川智士}},\\n\\tyear = {2014},\\n\\tnote = {Publisher: 公益社団法人 日本水産学会},\\n\\tpages = {837--837},\\n}\\n\\n\",\"author_short\":[\"今孝悦\",\"Khrueniam, U.\",\"有元貴文\",\"吉川尚\",\"岡本侑樹\",\"石川智士\"],\"key\":\"_1_2014\",\"id\":\"_1_2014\",\"bibbaseid\":\"-khrueniam-----1-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://ci.nii.ac.jp/naid/130004694420\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"book\",\"type\":\"book\",\"address\":\"東京\",\"title\":\"特集受精メカニズム新論争-ドグマの再構築\",\"isbn\":\"978-4-7809-0153-5\",\"language\":\"Japanese\",\"publisher\":\"学研メディカル秀潤社\",\"author\":[{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"伊川正人\"],\"suffixes\":[]}],\"year\":\"2014\",\"note\":\"OCLC: 874519589\",\"bibtex\":\"@book{_-_2014,\\n\\taddress = {東京},\\n\\ttitle = {特集受精メカニズム新論争-ドグマの再構築},\\n\\tisbn = {978-4-7809-0153-5},\\n\\tlanguage = {Japanese},\\n\\tpublisher = {学研メディカル秀潤社},\\n\\tauthor = {{伊川正人}},\\n\\tyear = {2014},\\n\\tnote = {OCLC: 874519589},\\n}\\n\\n\",\"author_short\":[\"伊川正人\"],\"key\":\"_-_2014\",\"id\":\"_-_2014\",\"bibbaseid\":\"--2014\",\"role\":\"author\",\"urls\":{},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"book\",\"type\":\"book\",\"address\":\"東京\",\"title\":\"今すぐ始めるゲノム編集: TALEN&CRISPR/Cas9の必須知識と実験プロトコール\",\"isbn\":\"978-4-7581-0190-5\",\"shorttitle\":\"今すぐ始めるゲノム編集\",\"language\":\"Japanese\",\"publisher\":\"羊土社\",\"author\":[{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"山本卓\"],\"suffixes\":[]}],\"year\":\"2014\",\"note\":\"OCLC: 875128657\",\"bibtex\":\"@book{__2014,\\n\\taddress = {東京},\\n\\ttitle = {今すぐ始めるゲノム編集: {TALEN}\\\\&{CRISPR}/{Cas9の必須知識と実験プロトコール}},\\n\\tisbn = {978-4-7581-0190-5},\\n\\tshorttitle = {今すぐ始めるゲノム編集},\\n\\tlanguage = {Japanese},\\n\\tpublisher = {羊土社},\\n\\tauthor = {{山本卓}},\\n\\tyear = {2014},\\n\\tnote = {OCLC: 875128657},\\n}\\n\\n\",\"author_short\":[\"山本卓\"],\"key\":\"__2014\",\"id\":\"__2014\",\"bibbaseid\":\"-talencrisprcas9-2014\",\"role\":\"author\",\"urls\":{},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"2. タイ南部バンドン湾の貝類養殖域における食物網構造\",\"volume\":\"80\",\"issn\":\"0021-5392\",\"url\":\"https://ci.nii.ac.jp/naid/130004694421\",\"abstract\":\"2. タイ南部バンドン湾の貝類養殖域における食物網構造 岡本 侑樹 , 石川 智士 , 今 孝悦 , 渡邉 一哉 , 吉川 尚 , SALAENOI JINTANA 日本水産学会誌 80(5), 838-838, 2014\",\"language\":\"en\",\"number\":\"5\",\"urldate\":\"2021-07-27\",\"journal\":\"日本水産学会誌\",\"author\":[{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"岡本侑樹\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"石川智士\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"今孝悦\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"渡邉一哉\"],\"suffixes\":[]},{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"吉川尚\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Salaenoi\"],\"firstnames\":[\"Jintana\"],\"suffixes\":[]}],\"year\":\"2014\",\"note\":\"Publisher: 公益社団法人 日本水産学会\",\"pages\":\"838–838\",\"bibtex\":\"@article{_2_2014,\\n\\ttitle = {2. タイ南部バンドン湾の貝類養殖域における食物網構造},\\n\\tvolume = {80},\\n\\tissn = {0021-5392},\\n\\turl = {https://ci.nii.ac.jp/naid/130004694421},\\n\\tabstract = {2. タイ南部バンドン湾の貝類養殖域における食物網構造 岡本 侑樹 , 石川 智士 , 今 孝悦 , 渡邉 一哉 , 吉川 尚 , SALAENOI JINTANA 日本水産学会誌 80(5), 838-838, 2014},\\n\\tlanguage = {en},\\n\\tnumber = {5},\\n\\turldate = {2021-07-27},\\n\\tjournal = {日本水産学会誌},\\n\\tauthor = {{岡本侑樹} and {石川智士} and {今孝悦} and {渡邉一哉} and {吉川尚} and Salaenoi, Jintana},\\n\\tyear = {2014},\\n\\tnote = {Publisher: 公益社団法人 日本水産学会},\\n\\tpages = {838--838},\\n}\\n\\n\",\"author_short\":[\"岡本侑樹\",\"石川智士\",\"今孝悦\",\"渡邉一哉\",\"吉川尚\",\"Salaenoi, J.\"],\"key\":\"_2_2014\",\"id\":\"_2_2014\",\"bibbaseid\":\"-----salaenoi-2-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://ci.nii.ac.jp/naid/130004694421\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"book\",\"type\":\"book\",\"address\":\"京都\",\"title\":\"動植物の受精学: 共通機構と多様性\",\"isbn\":\"978-4-7598-1514-6\",\"shorttitle\":\"動植物の受精学\",\"language\":\"Japanese\",\"publisher\":\"化学同人\",\"author\":[{\"firstnames\":[],\"propositions\":[],\"lastnames\":[\"沢田 均\"],\"suffixes\":[]}],\"year\":\"2014\",\"note\":\"OCLC: 882595519\",\"bibtex\":\"@book{___2014,\\n\\taddress = {京都},\\n\\ttitle = {動植物の受精学: 共通機構と多様性},\\n\\tisbn = {978-4-7598-1514-6},\\n\\tshorttitle = {動植物の受精学},\\n\\tlanguage = {Japanese},\\n\\tpublisher = {化学同人},\\n\\tauthor = {{沢田 均}},\\n\\tyear = {2014},\\n\\tnote = {OCLC: 882595519},\\n}\\n\\n\",\"author_short\":[\"沢田 均\"],\"key\":\"___2014\",\"id\":\"___2014\",\"bibbaseid\":\"--2014\",\"role\":\"author\",\"urls\":{},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"}],\n      metadata: {\"authorlinks\":{}},\n      ownerID: undefined\n    };\n  </script>\n\n  <script type=\"text/javascript\">\n  var site$;\n  if (typeof $ != 'undefined') {\n    console.log('existing jquery: storing and then restoring');\n    site$ = $;\n    $ = null;\n  }\n  </script>\n\n  <script src=\"https://ajax.googleapis.com/ajax/libs/jquery/2.2.4/jquery.min.js\">\n  </script>\n  <script type=\"text/javascript\">\n  if (site$) {\n    console.log('existing jquery: avoiding conflict and restoring');\n    bb$ = $.noConflict(true);\n    $ = site$;\n  } else {\n    bb$ = $;\n  }\n  </script>\n\n  <script src=\"//bibbase.org/js/bibbase.min.js\"\n          type=\"text/javascript\">\n  </script>\n\n  <script type=\"text/javascript\"\n          src=\"https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML\">\n  </script>\n  <script type=\"text/x-mathjax-config\">\n    MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\\\(','\\\\)']]},\n                        skipTags: [\"body\"],\n                        processClass: \"bibbase_paper\"});\n  </script>\n\n  <style>\n  @media print {\n    .dontprint {\n        display: none !important;\n    }\n\n  .bibbase_group {\n    background-color: #E0E0E0;\n    font-style: italic;\n    font-size: larger;\n    text-shadow: 0px 0px 3px #FFFFFF;\n    border-radius: 4px 4px 4px 4px;\n    -moz-border-radius: 4px 4px 4px 4px;\n    -webkit-border-radius: 4px;\n    padding: 5px 40px 5px;\n    margin-top: 10px;\n    margin-bottom: 5px;\n    cursor: pointer;\n  }\n\n  .bibbase_paper {\n    margin-bottom: 5px;\n  }\n\n  }\n  </style>\n\n  \n  <div style=\"float: right; margin-right: 10px; margin-top: 10px; text-align: right; font-size: 12px\">\n    generated by\n    <a href=\"//bibbase.org\"\n       onclick=\"trackOutboundLink('bibbase', 'logo clicked', window.location.href, '//bibbase.org'); return false;\">\n      <img src=\"//bibbase.org/img/logo.svg\"\n           style=\"vertical-align: middle; margin-left: 3px; height: 16px;\"/\n           alt=\"bibbase.org\"\n           title=\"BibBase – The easiest way to maintain your publications page.\"\n        ></a>\n    \n  </div>\n  \n\n  <div id=\"bibbase_header\" class=\"dontprint\" style=\"display: none\">\n\n    <ul class=\"nav nav-pills\" style=\"margin: 0px;\">\n\n      <li class=\"dropdown\" id=\"groupby_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\">\n          Group by\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li class=\"groupby year\"><a onclick=\"groupby('year')\">\n            Year</a>\n        </li>\n        <li class=\"groupby author\"><a onclick=\"groupby('author_short')\">\n            Author</a>\n        </li>\n        <li class=\"groupby type\"><a onclick=\"groupby('type')\">\n            Type</a>\n        </li>\n        <li class=\"groupby keyword\"><a onclick=\"groupby('keyword')\">\n            Keyword</a>\n        </li>\n        <li class=\"groupby downloads\"><a onclick=\"groupby('downloads')\">\n            Downloads</a>\n        </li>\n      </ul>\n      </li>\n\n\n      <li class=\"dropdown\" id=\"menu_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\" alt=\"controls\">\n          <i class=\"fa fa-reorder\" alt=\"controls\"></i>\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li>\n          <a onclick=\"toggleAll()\">\n            <i class=\"fa fa-chevron-down fa-fw\"></i> Expand/Collapse All\n          </a>\n        </li>\n        <li>\n          <a download=\"items\" href=\"data:text/bibtex;base64,@article{alavi_roles_2014,
	title = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},
	volume = {147},
	issn = {1470-1626, 1741-7899},
	url = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},
	doi = {10.1530/REP-13-0418},
	abstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (
              Ruditapes
              philippinarum
              ), Pacific oyster (
              Crassostrea gigas
              ), and Japanese scallop (
              Patinopecten yessoensis
              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K
              +
              /H
              +
              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K
              +
              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K
              +
              or ≥2.5 mM 4-aminopyridine, suggesting K
              +
              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca
              2
              +
              is required for Ca
              2
              +
              /calmodulin-dependent flagellar beating. Ca
              2
              +
              influx occurs via Ca
              2
              +
              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca
              2
              +
              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca
              2
              +
              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca
              2
              +
              channel blocker. Extracellular Na
              +
              is also essential for sperm motility initiation via regulation of Na
              +
              /Ca
              2
              +
              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K
              +
              , Ca
              2
              +
              , and Na
              +
              .},
	number = {3},
	urldate = {2021-07-27},
	journal = {REPRODUCTION},
	author = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},
	month = mar,
	year = {2014},
	pages = {331--345},
}



@article{alavi_protease_2014,
	title = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},
	volume = {40},
	issn = {0920-1742, 1573-5168},
	url = {http://link.springer.com/10.1007/s10695-014-9933-8},
	doi = {10.1007/s10695-014-9933-8},
	language = {en},
	number = {5},
	urldate = {2021-07-27},
	journal = {Fish Physiology and Biochemistry},
	author = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},
	month = oct,
	year = {2014},
	pages = {1393--1398},
}



@article{higuchi_biotic_2014,
	title = {Biotic {Control} of {Skeletal} {Growth} by {Scleractinian} {Corals} in {Aragonite}–{Calcite} {Seas}},
	volume = {9},
	issn = {1932-6203},
	url = {https://dx.plos.org/10.1371/journal.pone.0091021},
	doi = {10.1371/journal.pone.0091021},
	language = {en},
	number = {3},
	urldate = {2021-07-27},
	journal = {PLoS ONE},
	author = {Higuchi, Tomihiko and Fujimura, Hiroyuki and Yuyama, Ikuko and Harii, Saki and Agostini, Sylvain and Oomori, Tamotsu},
	editor = {Roberts, John Murray},
	month = mar,
	year = {2014},
	pages = {e91021},
}



@article{hiyama_sperm_2014,
	title = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\textit{{Coturnix} japonica})},
	volume = {147},
	issn = {1470-1626, 1741-7899},
	url = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},
	doi = {10.1530/REP-13-0439},
	abstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility
              in vitro
              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of
              HSP70
              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific
              in situ
              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization
              in vivo
              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},
	number = {2},
	urldate = {2021-07-27},
	journal = {REPRODUCTION},
	author = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},
	month = feb,
	year = {2014},
	pages = {167--178},
}



@incollection{sawada_structure_2014,
	address = {Tokyo},
	title = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},
	isbn = {978-4-431-54588-0 978-4-431-54589-7},
	url = {http://link.springer.com/10.1007/978-4-431-54589-7_5},
	language = {en},
	urldate = {2021-07-27},
	booktitle = {Sexual {Reproduction} in {Animals} and {Plants}},
	publisher = {Springer Japan},
	author = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},
	editor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},
	year = {2014},
	doi = {10.1007/978-4-431-54589-7_5},
	pages = {49--57},
}



@article{kamiya_nonreproductive_2014,
	title = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: {FUNCTIONS} {OF} {GNRH} {IN} {ASCIDIANS}},
	volume = {243},
	issn = {10588388},
	shorttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176},
	doi = {10.1002/dvdy.24176},
	language = {en},
	number = {12},
	urldate = {2021-07-27},
	journal = {Developmental Dynamics},
	author = {Kamiya, Chisato and Ohta, Naoyuki and Ogura, Yosuke and Yoshida, Keita and Horie, Takeo and Kusakabe, Takehiro G. and Satake, Honoo and Sasakura, Yasunori},
	month = dec,
	year = {2014},
	pages = {1524--1535},
}


@incollection{carroll_imaging_2014,
	address = {Totowa, NJ},
	title = {Imaging {Neural} {Development} in {Embryonic} and {Larval} {Sea} {Urchins}},
	volume = {1128},
	isbn = {978-1-62703-973-4 978-1-62703-974-1},
	url = {http://link.springer.com/10.1007/978-1-62703-974-1_9},
	urldate = {2021-07-27},
	booktitle = {Developmental {Biology} of the {Sea} {Urchin} and {Other} {Marine} {Invertebrates}},
	publisher = {Humana Press},
	author = {Krupke, Oliver and Yaguchi, Shunsuke and Yaguchi, Junko and Burke, Robert D.},
	editor = {Carroll, David J. and Stricker, Stephen A.},
	year = {2014},
	doi = {10.1007/978-1-62703-974-1_9},
	note = {Series Title: Methods in Molecular Biology},
	pages = {147--160},
}



@article{mizushima_birth_2014,
	title = {The birth of quail chicks after intracytoplasmic sperm injection},
	volume = {141},
	issn = {1477-9129, 0950-1991},
	url = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},
	doi = {10.1242/dev.111765},
	abstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},
	language = {en},
	number = {19},
	urldate = {2021-07-27},
	journal = {Development},
	author = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},
	month = oct,
	year = {2014},
	pages = {3799--3806},
}



@article{nakano_survey_2014,
	title = {Survey of the {Japanese} {Coast} {Reveals} {Abundant} {Placozoan} {Populations} in the {Northern} {Pacific} {Ocean}},
	volume = {4},
	issn = {2045-2322},
	url = {http://www.nature.com/articles/srep05356},
	doi = {10.1038/srep05356},
	language = {en},
	number = {1},
	urldate = {2021-07-27},
	journal = {Scientific Reports},
	author = {Nakano, Hiroaki},
	year = {2014},
	pages = {5356},
}



@article{sasaki_crisprcas9-mediated_2014,
	title = {{CRISPR}/{Cas9}-mediated gene knockout in the ascidian \textit{{Ciona} intestinalis}},
	volume = {56},
	issn = {0012-1592, 1440-169X},
	shorttitle = {{\textless}span style="font-variant},
	url = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149},
	doi = {10.1111/dgd.12149},
	language = {en},
	number = {7},
	urldate = {2021-07-27},
	journal = {Development, Growth \& Differentiation},
	author = {Sasaki, Haruka and Yoshida, Keita and Hozumi, Akiko and Sasakura, Yasunori},
	month = sep,
	year = {2014},
	pages = {499--510},
}



@incollection{sawada_germline_2014,
	address = {Tokyo},
	title = {Germline {Transformation} in the {Ascidian} \textit{{Ciona} intestinalis}},
	isbn = {978-4-431-54588-0 978-4-431-54589-7},
	url = {http://link.springer.com/10.1007/978-4-431-54589-7_38},
	language = {en},
	urldate = {2021-07-27},
	booktitle = {Sexual {Reproduction} in {Animals} and {Plants}},
	publisher = {Springer Japan},
	author = {Sasakura, Yasunori},
	editor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},
	year = {2014},
	doi = {10.1007/978-4-431-54589-7_38},
	pages = {465--473},
}



@article{shiba_distinct_2014,
	title = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \textit{{Ciona}} {Sperm}},
	volume = {15},
	issn = {1422-0067},
	url = {http://www.mdpi.com/1422-0067/15/8/13192},
	doi = {10.3390/ijms150813192},
	language = {en},
	number = {8},
	urldate = {2021-07-27},
	journal = {International Journal of Molecular Sciences},
	author = {Shiba, Kogiku and Inaba, Kazuo},
	month = jul,
	year = {2014},
	pages = {13192--13208},
}



@article{sung_species_2014,
	title = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\textrm{2}}$},
	volume = {137},
	issn = {09247963},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},
	doi = {10.1016/j.jmarsys.2014.04.013},
	language = {en},
	urldate = {2021-07-27},
	journal = {Journal of Marine Systems},
	author = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},
	month = sep,
	year = {2014},
	pages = {67--73},
}



@article{treen_tissue-specific_2014,
	title = {Tissue-specific and ubiquitous gene knockouts by {TALEN} electroporation provide new approaches to investigating gene function in \textit{{Ciona}}},
	volume = {141},
	issn = {1477-9129, 0950-1991},
	url = {https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by},
	doi = {10.1242/dev.099572},
	abstract = {Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.},
	language = {en},
	number = {2},
	urldate = {2021-07-27},
	journal = {Development},
	author = {Treen, Nicholas and Yoshida, Keita and Sakuma, Tetsushi and Sasaki, Haruka and Kawai, Narudo and Yamamoto, Takashi and Sasakura, Yasunori},
	month = jan,
	year = {2014},
	pages = {481--487},
}



@article{yokoe_sperm_2014,
	title = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \textit{{Cynops} pyrrhogaster}},
	volume = {15},
	issn = {1422-0067},
	url = {http://www.mdpi.com/1422-0067/15/9/15210},
	doi = {10.3390/ijms150915210},
	language = {en},
	number = {9},
	urldate = {2021-07-27},
	journal = {International Journal of Molecular Sciences},
	author = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},
	month = aug,
	year = {2014},
	pages = {15210--15224},
}



@article{yoshida_germ_2014,
	title = {Germ cell mutations of the ascidian \textit{{Ciona} intestinalis} with {TALE} nucleases: germ cell mutagenesis in \textit{ciona}},
	volume = {52},
	issn = {1526954X},
	shorttitle = {Germ cell mutations of the ascidian \textit{{Ciona} intestinalis} with {TALE} nucleases},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770},
	doi = {10.1002/dvg.22770},
	language = {en},
	number = {5},
	urldate = {2021-07-27},
	journal = {genesis},
	author = {Yoshida, Keita and Treen, Nicholas and Hozumi, Akiko and Sakuma, Tetsushi and Yamamoto, Takashi and Sasakura, Yasunori},
	month = may,
	year = {2014},
	pages = {431--439},
}



@article{_1_2014,
	title = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階},
	volume = {80},
	issn = {0021-5392},
	url = {https://ci.nii.ac.jp/naid/130004694420},
	abstract = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 今 孝悦 , KHRUENIAM UDOM , 有元 貴文 , 吉川 尚 , 岡本 侑樹 , 石川 智士 日本水産学会誌 80(5), 837-837, 2014},
	language = {en},
	number = {5},
	urldate = {2021-07-27},
	journal = {日本水産学会誌},
	author = {{今孝悦} and Khrueniam, Udom and {有元貴文} and {吉川尚} and {岡本侑樹} and {石川智士}},
	year = {2014},
	note = {Publisher: 公益社団法人 日本水産学会},
	pages = {837--837},
}



@book{_-_2014,
	address = {東京},
	title = {特集受精メカニズム新論争-ドグマの再構築},
	isbn = {978-4-7809-0153-5},
	language = {Japanese},
	publisher = {学研メディカル秀潤社},
	author = {{伊川正人}},
	year = {2014},
	note = {OCLC: 874519589},
}



@book{__2014,
	address = {東京},
	title = {今すぐ始めるゲノム編集: {TALEN}\&{CRISPR}/{Cas9の必須知識と実験プロトコール}},
	isbn = {978-4-7581-0190-5},
	shorttitle = {今すぐ始めるゲノム編集},
	language = {Japanese},
	publisher = {羊土社},
	author = {{山本卓}},
	year = {2014},
	note = {OCLC: 875128657},
}



@article{_2_2014,
	title = {2. タイ南部バンドン湾の貝類養殖域における食物網構造},
	volume = {80},
	issn = {0021-5392},
	url = {https://ci.nii.ac.jp/naid/130004694421},
	abstract = {2. タイ南部バンドン湾の貝類養殖域における食物網構造 岡本 侑樹 , 石川 智士 , 今 孝悦 , 渡邉 一哉 , 吉川 尚 , SALAENOI JINTANA 日本水産学会誌 80(5), 838-838, 2014},
	language = {en},
	number = {5},
	urldate = {2021-07-27},
	journal = {日本水産学会誌},
	author = {{岡本侑樹} and {石川智士} and {今孝悦} and {渡邉一哉} and {吉川尚} and Salaenoi, Jintana},
	year = {2014},
	note = {Publisher: 公益社団法人 日本水産学会},
	pages = {838--838},
}



@book{___2014,
	address = {京都},
	title = {動植物の受精学: 共通機構と多様性},
	isbn = {978-4-7598-1514-6},
	shorttitle = {動植物の受精学},
	language = {Japanese},
	publisher = {化学同人},
	author = {{沢田 均}},
	year = {2014},
	note = {OCLC: 882595519},
}

\">\n            <i class=\"fa fa-download fa-fw\"></i> Download BibTeX\n          </a>\n        </li>\n        <li>\n          <a href=\"//bibbase.org/rss?bib=https://api.zotero.org/users/3476755/collections/NJGLI74A/items?key=ckhubDoO51X1cQZZ35NkGdxk&amp;format=bibtex&amp;limit=100\">\n            <i class=\"fa fa-rss fa-fw\"></i> RSS Feed\n          </a>\n          </li>\n      </ul>\n      </li>\n\n      \n\n\n      \n    </ul>\n\n\n    <div class=\"alert alert-success bibbase_msg\" id=\"bibbase_msg_embed\"\n         style=\"display: none;\">\n\n      Excellent! Next you can\n      <a href=\"/network/register?next=/network/newSiteFromTemplate%3Fbiburl=https://api.zotero.org/users/3476755/collections/NJGLI74A/items?key=ckhubDoO51X1cQZZ35NkGdxk&amp;format=bibtex&amp;limit=100\"\n      >create a new website with this list</a>, or\n      embed it in an existing web page by copying &amp; pasting\n      any of the following snippets.\n\n      <div style=\"text-align: left; margin-top: 1em;\">\n        <strong>JavaScript</strong>\n        <span class=\"comment recommended\">(easiest)</span>\n        <div class=\"well well-small\">\n          <code>\n            &lt;script src=\"https://bibbase.org/show?bib=https%3A%2F%2Fapi.zotero.org%2Fusers%2F3476755%2Fcollections%2FNJGLI74A%2Fitems%3Fkey%3DckhubDoO51X1cQZZ35NkGdxk%26format%3Dbibtex%26limit%3D100&amp;jsonp=1&amp;authorFirst=1&amp;hidemenu=true&amp;commas=true&amp;sort=author_short&amp;groupby=&amp;jsonp=1\"&gt;&lt;/script&gt;\n          </code>\n        </div>\n\n        <strong>PHP</strong>\n        <div class=\"well well-small\">\n          <code>\n            &lt;?php\n            $contents = file_get_contents(\"https://bibbase.org/show?bib=https%3A%2F%2Fapi.zotero.org%2Fusers%2F3476755%2Fcollections%2FNJGLI74A%2Fitems%3Fkey%3DckhubDoO51X1cQZZ35NkGdxk%26format%3Dbibtex%26limit%3D100&amp;jsonp=1&amp;authorFirst=1&amp;hidemenu=true&amp;commas=true&amp;sort=author_short&amp;groupby=\");\n            print_r($contents);\n            ?&gt;\n          </code>\n        </div>\n\n        <strong>iFrame</strong>\n        <span class=\"comment\">(not recommended)</span>\n        <div class=\"well well-small\">\n          <code>\n            &lt;iframe src=\"https://bibbase.org/show?bib=https%3A%2F%2Fapi.zotero.org%2Fusers%2F3476755%2Fcollections%2FNJGLI74A%2Fitems%3Fkey%3DckhubDoO51X1cQZZ35NkGdxk%26format%3Dbibtex%26limit%3D100&amp;jsonp=1&amp;authorFirst=1&amp;hidemenu=true&amp;commas=true&amp;sort=author_short&amp;groupby=\"&gt;&lt;/iframe&gt;\n          </code>\n        </div>\n\n        <p>\n          For more details see the <a href=\"//bibbase.org/help\">documention</a>.\n        </p>\n      </div>\n    </div>\n\n    <div class=\"alert alert-success bibbase_msg\" id=\"bibbase_msg_preview\"\n         style=\"display: none;\">\n\n      This is a preview! To use this list on your own web site\n      or create a new web site from it,\n      <a href=\"/network/register?next=/network/newAccountWithFile%3FfileId=\"\n      >create a free account</a>. The file will be added\n      and you will be able to edit it in the File Manager.\n      We will show you instructions once you've created your account.\n    </div>\n\n    <div class=\"alert alert-warning bibbase_msg\" id=\"bibbase_msg_mendeley1\"\n         style=\"display: none;\">\n\n      <p>To the site owner:</p>\n\n      <p><strong>Action required!</strong> Mendeley is changing its\n        API. In order to keep using Mendeley with BibBase past April\n        14th, you need to:\n        <ol>\n          <li>renew the authorization for BibBase on Mendeley, and</li>\n          <li>update the BibBase URL\n            in your page the same way you did when you initially set up\n            this page.\n          </li>\n        </ol>\n      </p>\n\n      <p>\n        <a href=\"http://bibbase.org/cgi-bin/mendeley2/get.py\">\n          <i class=\"fa fa-angle-double-right\"></i>\n          Fix it now</a>\n      </p>\n    </div>\n\n  </div>\n\n\n  <div id=\"bibbase_body\">\n    \n  \n  <div class=\"bibbase_paper\" id=\"alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Alavi, S. M. H., Matsumura, N., Shiba, K., Itoh, N., Takahashi, K. G, Inaba, K.,  &amp; Osada, M.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml', event);\">\n    Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>REPRODUCTION</i>, 147(3): 331–345. March 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml\"\n     onclick=\"log_download('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Roles of extracellular ions and pH in 5-HT-induced sperm motility in marine bivalve [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1530/REP-13-0418\"\n     onclick=\"log_download('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014', 'http://doi.org/10.1530/REP-13-0418', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('alavi-matsumura-shiba-itoh-takahashi-inaba-osada-rolesofextracellularionsandphin5htinducedspermmotilityinmarinebivalve-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{alavi_roles_2014,\n\ttitle = {Roles of extracellular ions and {pH} in 5-{HT}-induced sperm motility in marine bivalve},\n\tvolume = {147},\n\tissn = {1470-1626, 1741-7899},\n\turl = {https://rep.bioscientifica.com/view/journals/rep/147/3/331.xml},\n\tdoi = {10.1530/REP-13-0418},\n\tabstract = {Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam (\n              Ruditapes\n              philippinarum\n              ), Pacific oyster (\n              Crassostrea gigas\n              ), and Japanese scallop (\n              Patinopecten yessoensis\n              ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K\n              +\n              /H\n              +\n              exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K\n              +\n              concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K\n              +\n              or ≥2.5 mM 4-aminopyridine, suggesting K\n              +\n              efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca\n              2\n              +\n              is required for Ca\n              2\n              +\n              /calmodulin-dependent flagellar beating. Ca\n              2\n              +\n              influx occurs via Ca\n              2\n              +\n              channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca\n              2\n              +\n              channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca\n              2\n              +\n              rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca\n              2\n              +\n              channel blocker. Extracellular Na\n              +\n              is also essential for sperm motility initiation via regulation of Na\n              +\n              /Ca\n              2\n              +\n              exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K\n              +\n              , Ca\n              2\n              +\n              , and Na\n              +\n              .},\n\tnumber = {3},\n\turldate = {2021-07-27},\n\tjournal = {REPRODUCTION},\n\tauthor = {Alavi, Sayyed Mohammad Hadi and Matsumura, Natsuki and Shiba, Kogiku and Itoh, Naoki and Takahashi, Keisuke G and Inaba, Kazuo and Osada, Makoto},\n\tmonth = mar,\n\tyear = {2014},\n\tpages = {331--345},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Factors that inhibit and stimulate the initiation of sperm motility were determined for Manila clam ( Ruditapes philippinarum ), Pacific oyster ( Crassostrea gigas ), and Japanese scallop ( Patinopecten yessoensis ). Compared with artificial seawater (ASW), serotonin (5-hydroxytryptamine creatinine sulfate, 5-HT) could fully trigger sperm motility and increase sperm velocity and motility duration. Sperm motility was decreased in ASW at pH 6.5–7.0 and suppressed at pH 4.0. In Manila clam and Pacific oyster, 5-HT could overcome the inhibitory effects of acidic pH on sperm motility. In the presence of nigericin (a K + /H + exchanger), sperm motility was only triggered at pH 8.3. Testicular fluid K + concentrations were two- to fourfold higher than that in ASW. Sperm motility and velocity were decreased in ASW or 5-HT containing ≥40 mM K + or ≥2.5 mM 4-aminopyridine, suggesting K + efflux requirement to initiate motility. Sperm motility and velocity were reduced in ASW or 5-HT containing EGTA or W-7, suggesting that extracellular Ca 2 + is required for Ca 2 + /calmodulin-dependent flagellar beating. Ca 2 + influx occurs via Ca 2 + channels because sperm motility and velocity were decreased in both ASW and 5-HT containing T-type and L-type Ca 2 + channel blockers. 5-HT-dependent initiation of sperm motility was associated with intracellular Ca 2 + rise, which was comparable to that seen in ASW but was not observed in the presence of EGTA or a Ca 2 + channel blocker. Extracellular Na + is also essential for sperm motility initiation via regulation of Na + /Ca 2 + exchange. Overall, 5-HT-dependent initiation of sperm motility in marine bivalve mollusks is an osmolality-independent mechanism and regulated by extracellular pH, K + , Ca 2 + , and Na + .\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Alavi, S. M. H., Postlerová-Maňásková, P., Hatef, A., Pšenička, M., Pěknicová, J., Inaba, K., Ciereszko, A.,  &amp; Linhart, O.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/s10695-014-9933-8\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://link.springer.com/10.1007/s10695-014-9933-8', event);\">\n    Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Fish Physiology and Biochemistry</i>, 40(5): 1393–1398. October 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/s10695-014-9933-8\"\n     onclick=\"log_download('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://link.springer.com/10.1007/s10695-014-9933-8', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/s10695-014-9933-8\"\n     onclick=\"log_download('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014', 'http://doi.org/10.1007/s10695-014-9933-8', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('alavi-postlerovmaskov-hatef-penika-pknicov-inaba-ciereszko-linhart-proteaseinsturgeonspermandtheeffectsofproteaseinhibitorsonspermmotilityandvelocity-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{alavi_protease_2014,\n\ttitle = {Protease in sturgeon sperm and the effects of protease inhibitors on sperm motility and velocity},\n\tvolume = {40},\n\tissn = {0920-1742, 1573-5168},\n\turl = {http://link.springer.com/10.1007/s10695-014-9933-8},\n\tdoi = {10.1007/s10695-014-9933-8},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {Fish Physiology and Biochemistry},\n\tauthor = {Alavi, Sayyed Mohammad Hadi and Postlerová-Maňásková, Pavla and Hatef, Azadeh and Pšenička, Martin and Pěknicová, Jana and Inaba, Kazuo and Ciereszko, Andrzej and Linhart, Otomar},\n\tmonth = oct,\n\tyear = {2014},\n\tpages = {1393--1398},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Higuchi, T., Fujimura, H., Yuyama, I., Harii, S., Agostini, S.,  &amp; Oomori, T.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://dx.plos.org/10.1371/journal.pone.0091021\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014', 'https://dx.plos.org/10.1371/journal.pone.0091021', event);\">\n    Biotic Control of Skeletal Growth by Scleractinian Corals in Aragonite–Calcite Seas.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>PLoS ONE</i>, 9(3): e91021. March 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://dx.plos.org/10.1371/journal.pone.0091021\"\n     onclick=\"log_download('higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014', 'https://dx.plos.org/10.1371/journal.pone.0091021', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Biotic Control of Skeletal Growth by Scleractinian Corals in Aragonite–Calcite Seas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1371/journal.pone.0091021\"\n     onclick=\"log_download('higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014', 'http://doi.org/10.1371/journal.pone.0091021', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>7 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('higuchi-fujimura-yuyama-harii-agostini-oomori-bioticcontrolofskeletalgrowthbyscleractiniancoralsinaragonitecalciteseas-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{higuchi_biotic_2014,\n\ttitle = {Biotic {Control} of {Skeletal} {Growth} by {Scleractinian} {Corals} in {Aragonite}–{Calcite} {Seas}},\n\tvolume = {9},\n\tissn = {1932-6203},\n\turl = {https://dx.plos.org/10.1371/journal.pone.0091021},\n\tdoi = {10.1371/journal.pone.0091021},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2021-07-27},\n\tjournal = {PLoS ONE},\n\tauthor = {Higuchi, Tomihiko and Fujimura, Hiroyuki and Yuyama, Ikuko and Harii, Saki and Agostini, Sylvain and Oomori, Tamotsu},\n\teditor = {Roberts, John Murray},\n\tmonth = mar,\n\tyear = {2014},\n\tpages = {e91021},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Hiyama, G., Matsuzaki, M., Mizushima, S., Dohra, H., Ikegami, K., Yoshimura, T., Shiba, K., Inaba, K.,  &amp; Sasanami, T.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml', event);\">\n    Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (<i>Coturnix japonica</i>).\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>REPRODUCTION</i>, 147(2): 167–178. February 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml\"\n     onclick=\"log_download('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (&lt;i&gt;Coturnix japonica&lt;/i&gt;) [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1530/REP-13-0439\"\n     onclick=\"log_download('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014', 'http://doi.org/10.1530/REP-13-0439', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('hiyama-matsuzaki-mizushima-dohra-ikegami-yoshimura-shiba-inaba-etal-spermactivationbyheatshockprotein70supportsthemigrationofspermreleasedfromspermstoragetubulesinjapanesequailicoturnixjaponicai-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{hiyama_sperm_2014,\n\ttitle = {Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in {Japanese} quail (\\textit{{Coturnix} japonica})},\n\tvolume = {147},\n\tissn = {1470-1626, 1741-7899},\n\turl = {https://rep.bioscientifica.com/view/journals/rep/147/2/167.xml},\n\tdoi = {10.1530/REP-13-0439},\n\tabstract = {Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility\n              in vitro\n              and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of\n              HSP70\n              mRNA in the UVJ increases before ovulation/oviposition. Gene-specific\n              in situ\n              hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization\n              in vivo\n              . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.},\n\tnumber = {2},\n\turldate = {2021-07-27},\n\tjournal = {REPRODUCTION},\n\tauthor = {Hiyama, Gen and Matsuzaki, Mei and Mizushima, Shusei and Dohra, Hideo and Ikegami, Keisuke and Yoshimura, Takashi and Shiba, Kogiku and Inaba, Kazuo and Sasanami, Tomohiro},\n\tmonth = feb,\n\tyear = {2014},\n\tpages = {167--178},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs. Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo . In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Inaba, K., Mizuno, K.,  &amp; Shiba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_5', event);\">\n    Structure, Function, and Phylogenetic Consideration of Calaxin.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  In Sawada, H., Inoue, N.,  &amp; Iwano, M., editor(s), <i>Sexual Reproduction in Animals and Plants</i>, pages 49–57. Springer Japan, Tokyo,  2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_5\"\n     onclick=\"log_download('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Structure, Function, and Phylogenetic Consideration of Calaxin [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/978-4-431-54589-7_5\"\n     onclick=\"log_download('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014', 'http://doi.org/10.1007/978-4-431-54589-7_5', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('inaba-mizuno-shiba-structurefunctionandphylogeneticconsiderationofcalaxin-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@incollection{sawada_structure_2014,\n\taddress = {Tokyo},\n\ttitle = {Structure, {Function}, and {Phylogenetic} {Consideration} of {Calaxin}},\n\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\n\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_5},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\n\tpublisher = {Springer Japan},\n\tauthor = {Inaba, Kazuo and Mizuno, Katsutoshi and Shiba, Kogiku},\n\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\n\tyear = {2014},\n\tdoi = {10.1007/978-4-431-54589-7_5},\n\tpages = {49--57},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Kamiya, C., Ohta, N., Ogura, Y., Yoshida, K., Horie, T., Kusakabe, T. G., Satake, H.,  &amp; Sasakura, Y.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014', 'https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176', event);\">\n    Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: FUNCTIONS OF GNRH IN ASCIDIANS.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Developmental Dynamics</i>, 243(12): 1524–1535. December 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176\"\n     onclick=\"log_download('kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014', 'https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: FUNCTIONS OF GNRH IN ASCIDIANS [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/dvdy.24176\"\n     onclick=\"log_download('kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014', 'http://doi.org/10.1002/dvdy.24176', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kamiya-ohta-ogura-yoshida-horie-kusakabe-satake-sasakura-nonreproductiveroleofgonadotropinreleasinghormoneinthecontrolofascidianmetamorphosisfunctionsofgnrhinascidians-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{kamiya_nonreproductive_2014,\n\ttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis: {FUNCTIONS} {OF} {GNRH} {IN} {ASCIDIANS}},\n\tvolume = {243},\n\tissn = {10588388},\n\tshorttitle = {Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvdy.24176},\n\tdoi = {10.1002/dvdy.24176},\n\tlanguage = {en},\n\tnumber = {12},\n\turldate = {2021-07-27},\n\tjournal = {Developmental Dynamics},\n\tauthor = {Kamiya, Chisato and Ohta, Naoyuki and Ogura, Yosuke and Yoshida, Keita and Horie, Takeo and Kusakabe, Takehiro G. and Satake, Honoo and Sasakura, Yasunori},\n\tmonth = dec,\n\tyear = {2014},\n\tpages = {1524--1535},\n}\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Krupke, O., Yaguchi, S., Yaguchi, J.,  &amp; Burke, R. D.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/978-1-62703-974-1_9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014', 'http://link.springer.com/10.1007/978-1-62703-974-1_9', event);\">\n    Imaging Neural Development in Embryonic and Larval Sea Urchins.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  In Carroll, D. J.,  &amp; Stricker, S. A., editor(s), <i>Developmental Biology of the Sea Urchin and Other Marine Invertebrates</i>, volume 1128, pages 147–160. Humana Press, Totowa, NJ,  2014.\n  <span class=\"note\">Series Title: Methods in Molecular Biology</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/978-1-62703-974-1_9\"\n     onclick=\"log_download('krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014', 'http://link.springer.com/10.1007/978-1-62703-974-1_9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Imaging Neural Development in Embryonic and Larval Sea Urchins [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/978-1-62703-974-1_9\"\n     onclick=\"log_download('krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014', 'http://doi.org/10.1007/978-1-62703-974-1_9', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('krupke-yaguchi-yaguchi-burke-imagingneuraldevelopmentinembryonicandlarvalseaurchins-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@incollection{carroll_imaging_2014,\n\taddress = {Totowa, NJ},\n\ttitle = {Imaging {Neural} {Development} in {Embryonic} and {Larval} {Sea} {Urchins}},\n\tvolume = {1128},\n\tisbn = {978-1-62703-973-4 978-1-62703-974-1},\n\turl = {http://link.springer.com/10.1007/978-1-62703-974-1_9},\n\turldate = {2021-07-27},\n\tbooktitle = {Developmental {Biology} of the {Sea} {Urchin} and {Other} {Marine} {Invertebrates}},\n\tpublisher = {Humana Press},\n\tauthor = {Krupke, Oliver and Yaguchi, Shunsuke and Yaguchi, Junko and Burke, Robert D.},\n\teditor = {Carroll, David J. and Stricker, Stephen A.},\n\tyear = {2014},\n\tdoi = {10.1007/978-1-62703-974-1_9},\n\tnote = {Series Title: Methods in Molecular Biology},\n\tpages = {147--160},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Mizushima, S., Hiyama, G., Shiba, K., Inaba, K., Dohra, H., Ono, T., Shimada, K.,  &amp; Sasanami, T.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic', event);\">\n    The birth of quail chicks after intracytoplasmic sperm injection.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Development</i>, 141(19): 3799–3806. October 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic\"\n     onclick=\"log_download('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The birth of quail chicks after intracytoplasmic sperm injection [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1242/dev.111765\"\n     onclick=\"log_download('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014', 'http://doi.org/10.1242/dev.111765', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>4 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('mizushima-hiyama-shiba-inaba-dohra-ono-shimada-sasanami-thebirthofquailchicksafterintracytoplasmicsperminjection-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{mizushima_birth_2014,\n\ttitle = {The birth of quail chicks after intracytoplasmic sperm injection},\n\tvolume = {141},\n\tissn = {1477-9129, 0950-1991},\n\turl = {https://journals.biologists.com/dev/article/141/19/3799/46472/The-birth-of-quail-chicks-after-intracytoplasmic},\n\tdoi = {10.1242/dev.111765},\n\tabstract = {Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.},\n\tlanguage = {en},\n\tnumber = {19},\n\turldate = {2021-07-27},\n\tjournal = {Development},\n\tauthor = {Mizushima, Shusei and Hiyama, Gen and Shiba, Kogiku and Inaba, Kazuo and Dohra, Hideo and Ono, Tamao and Shimada, Kiyoshi and Sasanami, Tomohiro},\n\tmonth = oct,\n\tyear = {2014},\n\tpages = {3799--3806},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors – phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) – all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Nakano, H.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://www.nature.com/articles/srep05356\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014', 'http://www.nature.com/articles/srep05356', event);\">\n    Survey of the Japanese Coast Reveals Abundant Placozoan Populations in the Northern Pacific Ocean.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Scientific Reports</i>, 4(1): 5356.  2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://www.nature.com/articles/srep05356\"\n     onclick=\"log_download('nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014', 'http://www.nature.com/articles/srep05356', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Survey of the Japanese Coast Reveals Abundant Placozoan Populations in the Northern Pacific Ocean [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1038/srep05356\"\n     onclick=\"log_download('nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014', 'http://doi.org/10.1038/srep05356', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('nakano-surveyofthejapanesecoastrevealsabundantplacozoanpopulationsinthenorthernpacificocean-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{nakano_survey_2014,\n\ttitle = {Survey of the {Japanese} {Coast} {Reveals} {Abundant} {Placozoan} {Populations} in the {Northern} {Pacific} {Ocean}},\n\tvolume = {4},\n\tissn = {2045-2322},\n\turl = {http://www.nature.com/articles/srep05356},\n\tdoi = {10.1038/srep05356},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-07-27},\n\tjournal = {Scientific Reports},\n\tauthor = {Nakano, Hiroaki},\n\tyear = {2014},\n\tpages = {5356},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sasaki, H., Yoshida, K., Hozumi, A.,  &amp; Sasakura, Y.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014', 'https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149', event);\">\n    CRISPR/Cas9-mediated gene knockout in the ascidian <i>Ciona intestinalis</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Development, Growth & Differentiation</i>, 56(7): 499–510. September 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149\"\n     onclick=\"log_download('sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014', 'https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"CRISPR/Cas9-mediated gene knockout in the ascidian &lt;i&gt;Ciona intestinalis&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1111/dgd.12149\"\n     onclick=\"log_download('sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014', 'http://doi.org/10.1111/dgd.12149', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sasaki-yoshida-hozumi-sasakura-crisprcas9mediatedgeneknockoutintheascidianicionaintestinalisi-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{sasaki_crisprcas9-mediated_2014,\n\ttitle = {{CRISPR}/{Cas9}-mediated gene knockout in the ascidian \\textit{{Ciona} intestinalis}},\n\tvolume = {56},\n\tissn = {0012-1592, 1440-169X},\n\tshorttitle = {{\\textless}span style=&quot;font-variant},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/dgd.12149},\n\tdoi = {10.1111/dgd.12149},\n\tlanguage = {en},\n\tnumber = {7},\n\turldate = {2021-07-27},\n\tjournal = {Development, Growth \\&amp; Differentiation},\n\tauthor = {Sasaki, Haruka and Yoshida, Keita and Hozumi, Akiko and Sasakura, Yasunori},\n\tmonth = sep,\n\tyear = {2014},\n\tpages = {499--510},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sasakura-germlinetransformationintheascidianicionaintestinalisi-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sasakura, Y.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_38\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sasakura-germlinetransformationintheascidianicionaintestinalisi-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_38', event);\">\n    Germline Transformation in the Ascidian <i>Ciona intestinalis</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  In Sawada, H., Inoue, N.,  &amp; Iwano, M., editor(s), <i>Sexual Reproduction in Animals and Plants</i>, pages 465–473. Springer Japan, Tokyo,  2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://link.springer.com/10.1007/978-4-431-54589-7_38\"\n     onclick=\"log_download('sasakura-germlinetransformationintheascidianicionaintestinalisi-2014', 'http://link.springer.com/10.1007/978-4-431-54589-7_38', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Germline Transformation in the Ascidian &lt;i&gt;Ciona intestinalis&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/978-4-431-54589-7_38\"\n     onclick=\"log_download('sasakura-germlinetransformationintheascidianicionaintestinalisi-2014', 'http://doi.org/10.1007/978-4-431-54589-7_38', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sasakura-germlinetransformationintheascidianicionaintestinalisi-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sasakura-germlinetransformationintheascidianicionaintestinalisi-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@incollection{sawada_germline_2014,\n\taddress = {Tokyo},\n\ttitle = {Germline {Transformation} in the {Ascidian} \\textit{{Ciona} intestinalis}},\n\tisbn = {978-4-431-54588-0 978-4-431-54589-7},\n\turl = {http://link.springer.com/10.1007/978-4-431-54589-7_38},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tbooktitle = {Sexual {Reproduction} in {Animals} and {Plants}},\n\tpublisher = {Springer Japan},\n\tauthor = {Sasakura, Yasunori},\n\teditor = {Sawada, Hitoshi and Inoue, Naokazu and Iwano, Megumi},\n\tyear = {2014},\n\tdoi = {10.1007/978-4-431-54589-7_38},\n\tpages = {465--473},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K.,  &amp; Inaba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/8/13192\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://www.mdpi.com/1422-0067/15/8/13192', event);\">\n    Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in <i>Ciona</i> Sperm.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 15(8): 13192–13208. July 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/8/13192\"\n     onclick=\"log_download('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://www.mdpi.com/1422-0067/15/8/13192', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in &lt;i&gt;Ciona&lt;/i&gt; Sperm [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms150813192\"\n     onclick=\"log_download('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014', 'http://doi.org/10.3390/ijms150813192', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-inaba-distinctrolesofsolubleandtransmembraneadenylylcyclasesintheregulationofflagellarmotilityinicionaisperm-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_distinct_2014,\n\ttitle = {Distinct {Roles} of {Soluble} and {Transmembrane} {Adenylyl} {Cyclases} in the {Regulation} of {Flagellar} {Motility} in \\textit{{Ciona}} {Sperm}},\n\tvolume = {15},\n\tissn = {1422-0067},\n\turl = {http://www.mdpi.com/1422-0067/15/8/13192},\n\tdoi = {10.3390/ijms150813192},\n\tlanguage = {en},\n\tnumber = {8},\n\turldate = {2021-07-27},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = jul,\n\tyear = {2014},\n\tpages = {13192--13208},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sung, C., Kim, T. W., Park, Y., Kang, S., Inaba, K., Shiba, K., Choi, T. S., Moon, S., Litvin, S., Lee, K.,  &amp; Lee, J.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992', event);\">\n    Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\textrm{2}}$.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Journal of Marine Systems</i>, 137: 67–73. September 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992\"\n     onclick=\"log_download('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Species and gamete-specific fertilization success of two sea urchins under near future levels of pCO$_{\\textrm{2}}$ [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.jmarsys.2014.04.013\"\n     onclick=\"log_download('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014', 'http://doi.org/10.1016/j.jmarsys.2014.04.013', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sung-kim-park-kang-inaba-shiba-choi-moon-etal-speciesandgametespecificfertilizationsuccessoftwoseaurchinsundernearfuturelevelsofpcotextrm2-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{sung_species_2014,\n\ttitle = {Species and gamete-specific fertilization success of two sea urchins under near future levels of {pCO}$_{\\textrm{2}}$},\n\tvolume = {137},\n\tissn = {09247963},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0924796314000992},\n\tdoi = {10.1016/j.jmarsys.2014.04.013},\n\tlanguage = {en},\n\turldate = {2021-07-27},\n\tjournal = {Journal of Marine Systems},\n\tauthor = {Sung, Chan-Gyung and Kim, Tae Won and Park, Young-Gyu and Kang, Seong-Gil and Inaba, Kazuo and Shiba, Kogiku and Choi, Tae Seob and Moon, Seong-Dae and Litvin, Steve and Lee, Kyu-Tae and Lee, Jung-Suk},\n\tmonth = sep,\n\tyear = {2014},\n\tpages = {67--73},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T.,  &amp; Sasakura, Y.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014', 'https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by', event);\">\n    Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in <i>Ciona</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Development</i>, 141(2): 481–487. January 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by\"\n     onclick=\"log_download('treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014', 'https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in &lt;i&gt;Ciona&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1242/dev.099572\"\n     onclick=\"log_download('treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014', 'http://doi.org/10.1242/dev.099572', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('treen-yoshida-sakuma-sasaki-kawai-yamamoto-sasakura-tissuespecificandubiquitousgeneknockoutsbytalenelectroporationprovidenewapproachestoinvestigatinggenefunctioninicionai-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{treen_tissue-specific_2014,\n\ttitle = {Tissue-specific and ubiquitous gene knockouts by {TALEN} electroporation provide new approaches to investigating gene function in \\textit{{Ciona}}},\n\tvolume = {141},\n\tissn = {1477-9129, 0950-1991},\n\turl = {https://journals.biologists.com/dev/article/141/2/481/46452/Tissue-specific-and-ubiquitous-gene-knockouts-by},\n\tdoi = {10.1242/dev.099572},\n\tabstract = {Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.},\n\tlanguage = {en},\n\tnumber = {2},\n\turldate = {2021-07-27},\n\tjournal = {Development},\n\tauthor = {Treen, Nicholas and Yoshida, Keita and Sakuma, Tetsushi and Sasaki, Haruka and Kawai, Narudo and Yamamoto, Takashi and Sasakura, Yasunori},\n\tmonth = jan,\n\tyear = {2014},\n\tpages = {481--487},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Custom designed nucleases can simplify gene targeting experiments and have the potential to allow these techniques to be performed in a wide range of organisms. Transcriptional activator-like effector nucleases (TALENs) are starting to fulfill this potential with the advantages of low cost and fast construction times. Here, we report that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis. In Ciona there are well-established methods to introduce exogenous DNA by electroporation, and we show that this method can be used to introduce constructs that can express TALENs ubiquitously or in specific tissues. Our current protocols enable the rapid analysis of hundreds of TALEN-induced mutants. TALEN electroporations result in a high rate of mutations. These mutations can result in gene knockouts that recapitulate previously described functions of Fgf3 and Hox12. We show that TALENs can work efficiently to cause tissue-specific knockouts and demonstrate this by knocking out Hox12 in the epidermis and Fgf3 in neural tissues. We also use tissue-specific knockouts to reveal a new function of Fgf3 during ascidian larval metamorphosis.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Yokoe, M., Sano, M., Shibata, H., Shibata, D., Takayama-Watanabe, E., Inaba, K.,  &amp; Watanabe, A.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/9/15210\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://www.mdpi.com/1422-0067/15/9/15210', event);\">\n    Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, <i>Cynops pyrrhogaster</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 15(9): 15210–15224. August 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"http://www.mdpi.com/1422-0067/15/9/15210\"\n     onclick=\"log_download('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://www.mdpi.com/1422-0067/15/9/15210', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Sperm Proteases that May Be Involved in the Initiation of Sperm Motility in the Newt, &lt;i&gt;Cynops pyrrhogaster&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms150915210\"\n     onclick=\"log_download('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014', 'http://doi.org/10.3390/ijms150915210', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('yokoe-sano-shibata-shibata-takayamawatanabe-inaba-watanabe-spermproteasesthatmaybeinvolvedintheinitiationofspermmotilityinthenewticynopspyrrhogasteri-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{yokoe_sperm_2014,\n\ttitle = {Sperm {Proteases} that {May} {Be} {Involved} in the {Initiation} of {Sperm} {Motility} in the {Newt}, \\textit{{Cynops} pyrrhogaster}},\n\tvolume = {15},\n\tissn = {1422-0067},\n\turl = {http://www.mdpi.com/1422-0067/15/9/15210},\n\tdoi = {10.3390/ijms150915210},\n\tlanguage = {en},\n\tnumber = {9},\n\turldate = {2021-07-27},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Yokoe, Misato and Sano, Makoto and Shibata, Honami and Shibata, Daisuke and Takayama-Watanabe, Eriko and Inaba, Kazuo and Watanabe, Akihiko},\n\tmonth = aug,\n\tyear = {2014},\n\tpages = {15210--15224},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Yoshida, K., Treen, N., Hozumi, A., Sakuma, T., Yamamoto, T.,  &amp; Sasakura, Y.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014', 'https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770', event);\">\n    Germ cell mutations of the ascidian <i>Ciona intestinalis</i> with TALE nucleases: germ cell mutagenesis in <i>ciona</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>genesis</i>, 52(5): 431–439. May 2014.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770\"\n     onclick=\"log_download('yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014', 'https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Germ cell mutations of the ascidian &lt;i&gt;Ciona intestinalis&lt;/i&gt; with TALE nucleases: germ cell mutagenesis in &lt;i&gt;ciona&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/dvg.22770\"\n     onclick=\"log_download('yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014', 'http://doi.org/10.1002/dvg.22770', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('yoshida-treen-hozumi-sakuma-yamamoto-sasakura-germcellmutationsoftheascidianicionaintestinalisiwithtalenucleasesgermcellmutagenesisinicionai-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{yoshida_germ_2014,\n\ttitle = {Germ cell mutations of the ascidian \\textit{{Ciona} intestinalis} with {TALE} nucleases: germ cell mutagenesis in \\textit{ciona}},\n\tvolume = {52},\n\tissn = {1526954X},\n\tshorttitle = {Germ cell mutations of the ascidian \\textit{{Ciona} intestinalis} with {TALE} nucleases},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.22770},\n\tdoi = {10.1002/dvg.22770},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {genesis},\n\tauthor = {Yoshida, Keita and Treen, Nicholas and Hozumi, Akiko and Sakuma, Tetsushi and Yamamoto, Takashi and Sasakura, Yasunori},\n\tmonth = may,\n\tyear = {2014},\n\tpages = {431--439},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"-khrueniam-----1-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  今孝悦, Khrueniam, U., 有元貴文, 吉川尚, 岡本侑樹,  &amp; 石川智士\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://ci.nii.ac.jp/naid/130004694420\"\n     onclick=\"return trackOutboundLink('publications', 'click', '-khrueniam-----1-2014', 'https://ci.nii.ac.jp/naid/130004694420', event);\">\n    1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>日本水産学会誌</i>, 80(5): 837–837.  2014.\n  <span class=\"note\">Publisher: 公益社団法人 日本水産学会</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://ci.nii.ac.jp/naid/130004694420\"\n     onclick=\"log_download('-khrueniam-----1-2014', 'https://ci.nii.ac.jp/naid/130004694420', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/-khrueniam-----1-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('-khrueniam-----1-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{_1_2014,\n\ttitle = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階},\n\tvolume = {80},\n\tissn = {0021-5392},\n\turl = {https://ci.nii.ac.jp/naid/130004694420},\n\tabstract = {1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 今 孝悦 , KHRUENIAM UDOM , 有元 貴文 , 吉川 尚 , 岡本 侑樹 , 石川 智士 日本水産学会誌 80(5), 837-837, 2014},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {日本水産学会誌},\n\tauthor = {{今孝悦} and Khrueniam, Udom and {有元貴文} and {吉川尚} and {岡本侑樹} and {石川智士}},\n\tyear = {2014},\n\tnote = {Publisher: 公益社団法人 日本水産学会},\n\tpages = {837--837},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  1. タイ・ラヨーン沿岸における定置網漁獲物の栄養段階 今 孝悦 , KHRUENIAM UDOM , 有元 貴文 , 吉川 尚 , 岡本 侑樹 , 石川 智士 日本水産学会誌 80(5), 837-837, 2014\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"--2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  伊川正人\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  特集受精メカニズム新論争-ドグマの再構築.\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  学研メディカル秀潤社, 東京,  2014.\n  <span class=\"note\">OCLC: 874519589</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/--2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('--2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@book{_-_2014,\n\taddress = {東京},\n\ttitle = {特集受精メカニズム新論争-ドグマの再構築},\n\tisbn = {978-4-7809-0153-5},\n\tlanguage = {Japanese},\n\tpublisher = {学研メディカル秀潤社},\n\tauthor = {{伊川正人}},\n\tyear = {2014},\n\tnote = {OCLC: 874519589},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"-talencrisprcas9-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  山本卓\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  今すぐ始めるゲノム編集: TALEN&CRISPR/Cas9の必須知識と実験プロトコール.\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  羊土社, 東京,  2014.\n  <span class=\"note\">OCLC: 875128657</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/-talencrisprcas9-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('-talencrisprcas9-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@book{__2014,\n\taddress = {東京},\n\ttitle = {今すぐ始めるゲノム編集: {TALEN}\\&amp;{CRISPR}/{Cas9の必須知識と実験プロトコール}},\n\tisbn = {978-4-7581-0190-5},\n\tshorttitle = {今すぐ始めるゲノム編集},\n\tlanguage = {Japanese},\n\tpublisher = {羊土社},\n\tauthor = {{山本卓}},\n\tyear = {2014},\n\tnote = {OCLC: 875128657},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"-----salaenoi-2-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  岡本侑樹, 石川智士, 今孝悦, 渡邉一哉, 吉川尚,  &amp; Salaenoi, J.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://ci.nii.ac.jp/naid/130004694421\"\n     onclick=\"return trackOutboundLink('publications', 'click', '-----salaenoi-2-2014', 'https://ci.nii.ac.jp/naid/130004694421', event);\">\n    2. タイ南部バンドン湾の貝類養殖域における食物網構造.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>日本水産学会誌</i>, 80(5): 838–838.  2014.\n  <span class=\"note\">Publisher: 公益社団法人 日本水産学会</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://ci.nii.ac.jp/naid/130004694421\"\n     onclick=\"log_download('-----salaenoi-2-2014', 'https://ci.nii.ac.jp/naid/130004694421', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"2. タイ南部バンドン湾の貝類養殖域における食物網構造 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/-----salaenoi-2-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('-----salaenoi-2-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{_2_2014,\n\ttitle = {2. タイ南部バンドン湾の貝類養殖域における食物網構造},\n\tvolume = {80},\n\tissn = {0021-5392},\n\turl = {https://ci.nii.ac.jp/naid/130004694421},\n\tabstract = {2. タイ南部バンドン湾の貝類養殖域における食物網構造 岡本 侑樹 , 石川 智士 , 今 孝悦 , 渡邉 一哉 , 吉川 尚 , SALAENOI JINTANA 日本水産学会誌 80(5), 838-838, 2014},\n\tlanguage = {en},\n\tnumber = {5},\n\turldate = {2021-07-27},\n\tjournal = {日本水産学会誌},\n\tauthor = {{岡本侑樹} and {石川智士} and {今孝悦} and {渡邉一哉} and {吉川尚} and Salaenoi, Jintana},\n\tyear = {2014},\n\tnote = {Publisher: 公益社団法人 日本水産学会},\n\tpages = {838--838},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  2. タイ南部バンドン湾の貝類養殖域における食物網構造 岡本 侑樹 , 石川 智士 , 今 孝悦 , 渡邉 一哉 , 吉川 尚 , SALAENOI JINTANA 日本水産学会誌 80(5), 838-838, 2014\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"--2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  沢田 均\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  動植物の受精学: 共通機構と多様性.\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  化学同人, 京都,  2014.\n  <span class=\"note\">OCLC: 882595519</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/--2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('--2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@book{___2014,\n\taddress = {京都},\n\ttitle = {動植物の受精学: 共通機構と多様性},\n\tisbn = {978-4-7598-1514-6},\n\tshorttitle = {動植物の受精学},\n\tlanguage = {Japanese},\n\tpublisher = {化学同人},\n\tauthor = {{沢田 均}},\n\tyear = {2014},\n\tnote = {OCLC: 882595519},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n  </div>\n\n\n  <!-- Modal -->\n\n  <!-- <iframe id=\"bibbase_network_frame\" -->\n  <!--         src=\"//bibbase.org/network/control\" -->\n  <!--         style=\"display: none;\"> -->\n  <!-- </iframe> -->\n\n</div>\n"}; document.write(bibbase_data.data);