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\n\n \n \n \n \n \n \n Screening of a Chemical Library by HT-G4-FID for Discovery of Selective G-quadruplex Binders.\n \n \n \n \n\n\n \n Largy, E.; Saettel, N.; Hamon, F.; Dubruille, S.; and Teulade-Fichou, M.\n\n\n \n\n\n\n
Current Pharmaceutical Design, 18(14): 1992–2001. March 2012.\n
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@article{largy_screening_2012,\n\ttitle = {Screening of a {Chemical} {Library} by {HT}-{G4}-{FID} for {Discovery} of {Selective} {G}-quadruplex {Binders}},\n\tvolume = {18},\n\tcopyright = {All rights reserved},\n\tissn = {13816128},\n\turl = {http://www.eurekaselect.com/openurl/content.php?genre=article&issn=1381-6128&volume=18&issue=14&spage=1992},\n\tdoi = {10.2174/138161212799958350},\n\tlanguage = {en},\n\tnumber = {14},\n\turldate = {2024-01-05},\n\tjournal = {Current Pharmaceutical Design},\n\tauthor = {Largy, Eric and Saettel, Nicolas and Hamon, Florian and Dubruille, Sylvie and Teulade-Fichou, Marie-Paule},\n\tmonth = mar,\n\tyear = {2012},\n\tpages = {1992--2001},\n}\n\n
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\n\n \n \n \n \n \n \n Visualizing the Quadruplex: From Fluorescent Ligands to Light-Up Probes.\n \n \n \n \n\n\n \n Largy, E.; Granzhan, A.; Hamon, F.; Verga, D.; and Teulade-Fichou, M.\n\n\n \n\n\n\n In Chaires, J. B.; and Graves, D., editor(s),
Quadruplex Nucleic Acids, volume 330, pages 111–177. Springer Berlin Heidelberg, Berlin, Heidelberg, 2012.\n
Series Title: Topics in Current Chemistry\n\n
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@incollection{chaires_visualizing_2012,\n\taddress = {Berlin, Heidelberg},\n\ttitle = {Visualizing the {Quadruplex}: {From} {Fluorescent} {Ligands} to {Light}-{Up} {Probes}},\n\tvolume = {330},\n\tcopyright = {All rights reserved},\n\tisbn = {978-3-642-34742-9 978-3-642-34743-6},\n\tshorttitle = {Visualizing the {Quadruplex}},\n\turl = {https://link.springer.com/10.1007/128_2012_346},\n\tlanguage = {en},\n\turldate = {2024-01-05},\n\tbooktitle = {Quadruplex {Nucleic} {Acids}},\n\tpublisher = {Springer Berlin Heidelberg},\n\tauthor = {Largy, Eric and Granzhan, Anton and Hamon, Florian and Verga, Daniela and Teulade-Fichou, Marie-Paule},\n\teditor = {Chaires, Jonathan B. and Graves, David},\n\tyear = {2012},\n\tdoi = {10.1007/128_2012_346},\n\tnote = {Series Title: Topics in Current Chemistry},\n\tpages = {111--177},\n}\n\n
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\n\n \n \n \n \n \n \n Cationic pentaheteroaryls as selective G-quadruplex ligands by solvent-free microwave-assisted synthesis.\n \n \n \n \n\n\n \n Petenzi, M.; Verga, D.; Largy, E.; Hamon, F.; Doria, F.; Teulade-Fichou, M.; Guédin, A.; Mergny, J.; Mella, M.; and Freccero, M.\n\n\n \n\n\n\n
Chemistry - A European Journal, 18(45): 14487–96. November 2012.\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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@article{Petenzi2012,\n\ttitle = {Cationic pentaheteroaryls as selective {G}-quadruplex ligands by solvent-free microwave-assisted synthesis.},\n\tvolume = {18},\n\tcopyright = {All rights reserved},\n\tissn = {1521-3765},\n\turl = {http://doi.wiley.com/10.1002/chem.201202097 http://www.ncbi.nlm.nih.gov/pubmed/22996572},\n\tdoi = {10.1002/chem.201202097},\n\tabstract = {We report herein a solvent-free and microwaved-assisted synthesis of several water soluble acyclic pentaheteroaryls containing 1,2,4-oxadiazole moieties (1-7). Their binding interactions with DNA quadruplex structures were thoroughly investigated by FRET melting, fluorescent intercalator displacement assay (G4-FID) and CD spectroscopy. Among the G-quadruplexes considered, attention was focused on telomeric repeats together with the proto-oncogenic c-kit sequences and the c-myc oncogene promoter. Compound 1, and to a lesser extent 2 and 5, preferentially stabilise an antiparallel structure of the telomeric DNA motif, and exhibit an opposite binding behaviour to structurally related polyoxazole (TOxaPy), and do not bind duplex DNA. The efficiency and selectivity of the binding process was remarkably controlled by the structure of the solubilising moieties.},\n\tnumber = {45},\n\tjournal = {Chemistry - A European Journal},\n\tauthor = {Petenzi, Michele and Verga, Daniela and Largy, Eric and Hamon, Florian and Doria, Filippo and Teulade-Fichou, Marie-Paule and Guédin, Aurore and Mergny, Jean-Louis and Mella, Mariella and Freccero, Mauro},\n\tmonth = nov,\n\tyear = {2012},\n\tpmid = {22996572},\n\tkeywords = {1, 2, 4-oxadiazoles, Cations, Cations: chemistry, Circular Dichroism, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Fluorescent Dyes: chemistry, G-Quadruplexes, Ligands, Microwaves, Oxadiazoles, Oxadiazoles: chemistry, Water, Water: chemistry},\n\tpages = {14487--96},\n}\n\n
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\n We report herein a solvent-free and microwaved-assisted synthesis of several water soluble acyclic pentaheteroaryls containing 1,2,4-oxadiazole moieties (1-7). Their binding interactions with DNA quadruplex structures were thoroughly investigated by FRET melting, fluorescent intercalator displacement assay (G4-FID) and CD spectroscopy. Among the G-quadruplexes considered, attention was focused on telomeric repeats together with the proto-oncogenic c-kit sequences and the c-myc oncogene promoter. Compound 1, and to a lesser extent 2 and 5, preferentially stabilise an antiparallel structure of the telomeric DNA motif, and exhibit an opposite binding behaviour to structurally related polyoxazole (TOxaPy), and do not bind duplex DNA. The efficiency and selectivity of the binding process was remarkably controlled by the structure of the solubilising moieties.\n
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\n\n \n \n \n \n \n \n Screening for Quadruplex Binding Ligands: A Game of Chance?.\n \n \n \n \n\n\n \n Largy, E.; and Teulade-Fichou, M.\n\n\n \n\n\n\n In Spindler, L.; and Fritzsche, W., editor(s),
Guanine Quartets, pages 248–262. Royal Society of Chemistry, Cambridge, 2012.\n
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@incollection{spindler_screening_2012,\n\taddress = {Cambridge},\n\ttitle = {Screening for {Quadruplex} {Binding} {Ligands}: {A} {Game} of {Chance}?},\n\tcopyright = {All rights reserved},\n\tisbn = {978-1-84973-460-8},\n\tshorttitle = {Screening for {Quadruplex} {Binding} {Ligands}},\n\turl = {http://ebook.rsc.org/?DOI=10.1039/9781849736954-00248},\n\tlanguage = {en},\n\turldate = {2020-11-08},\n\tbooktitle = {Guanine {Quartets}},\n\tpublisher = {Royal Society of Chemistry},\n\tauthor = {Largy, E. and Teulade-Fichou, M.-P.},\n\teditor = {Spindler, Lea and Fritzsche, Wolfgang},\n\tyear = {2012},\n\tdoi = {10.1039/9781849736954-00248},\n\tpages = {248--262},\n}\n\n
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\n\n \n \n \n \n \n \n A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes.\n \n \n \n \n\n\n \n Largy, E.; Hamon, F.; and Teulade-Fichou, M.\n\n\n \n\n\n\n
Methods, 57(1): 129–137. May 2012.\n
publisher: Elsevier Inc.\n\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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@article{largy_streptavidin_2012,\n\ttitle = {A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes},\n\tvolume = {57},\n\tcopyright = {All rights reserved},\n\tissn = {10462023},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S1046202312000321},\n\tdoi = {10.1016/j.ymeth.2012.02.008},\n\tabstract = {Although quadruplex nucleic acids are thought to be involved in many biological processes, they are massively overwhelmed by duplex DNA in the cell. Small molecules, able to probe quadruplex nucleic acids with high optical selectivity, could possibly achieve the visualization of these processes. The aim of the method described herein is to evaluate quickly the optical selectivity of quadruplex nucleic acid probes, in isothermal conditions, using widely available materials, small quantities of oligonucleotides and virtually any kind and quantity of biological competitor. The assay relies on the use of streptavidin-coated paramagnetic particles and biotinylated quadruplex forming oligonucleotides, allowing a quick and easy separation of the quadruplex target from the competitor. In the present study, two quadruplex nucleic acids (the DNA and RNA human telomeric repeats) have been used as targets while a duplex DNA oligonucleotide, total DNA, total RNA, another quadruplex nucleic acid and a protein have been used as competitors. The optical selectivity of various probes, displaying different photophysical properties and binding selectivities, has been successfully examined, allowing the identification of a best candidate for further cell microscopy experiments. This assay allows a quick and reliable assessment of the labeling properties of a quadruplex binder in cellular environment conditions. It is an interesting alternative to gel electrophoresis experiments since it is performed in solution, has a well-resolved separation system and allows easy quantifications.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2020-11-09},\n\tjournal = {Methods},\n\tauthor = {Largy, Eric and Hamon, Florian and Teulade-Fichou, Marie-Paule},\n\tmonth = may,\n\tyear = {2012},\n\tpmid = {22406492},\n\tnote = {publisher: Elsevier Inc.},\n\tkeywords = {DNA, DNA: chemistry, Fluorescent Dyes, Fluorescent Dyes: chemistry, Fluorescent probe, G-Quadruplexes, G-quadruplex, Humans, Nucleic acids, Oligonucleotides, Oligonucleotides: chemistry, Param, RNA, RNA: chemistry, Streptavidin, Streptavidin: chemistry},\n\tpages = {129--137},\n}\n\n
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\n Although quadruplex nucleic acids are thought to be involved in many biological processes, they are massively overwhelmed by duplex DNA in the cell. Small molecules, able to probe quadruplex nucleic acids with high optical selectivity, could possibly achieve the visualization of these processes. The aim of the method described herein is to evaluate quickly the optical selectivity of quadruplex nucleic acid probes, in isothermal conditions, using widely available materials, small quantities of oligonucleotides and virtually any kind and quantity of biological competitor. The assay relies on the use of streptavidin-coated paramagnetic particles and biotinylated quadruplex forming oligonucleotides, allowing a quick and easy separation of the quadruplex target from the competitor. In the present study, two quadruplex nucleic acids (the DNA and RNA human telomeric repeats) have been used as targets while a duplex DNA oligonucleotide, total DNA, total RNA, another quadruplex nucleic acid and a protein have been used as competitors. The optical selectivity of various probes, displaying different photophysical properties and binding selectivities, has been successfully examined, allowing the identification of a best candidate for further cell microscopy experiments. This assay allows a quick and reliable assessment of the labeling properties of a quadruplex binder in cellular environment conditions. It is an interesting alternative to gel electrophoresis experiments since it is performed in solution, has a well-resolved separation system and allows easy quantifications.\n
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\n\n \n \n \n \n \n \n Effects of a halogenated G-quadruplex ligand from the pyridine dicarboxamide series on the terminal sequence of XpYp telomere in HT1080 cells.\n \n \n \n \n\n\n \n Sidibe, A.; Hamon, F.; Largy, E.; Gomez, D.; Teulade-Fichou, M.; Trentesaux, C.; and Riou, J.\n\n\n \n\n\n\n
Biochimie, 94(12): 1–10. July 2012.\n
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Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n
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@article{Sidibe2012,\n\ttitle = {Effects of a halogenated {G}-quadruplex ligand from the pyridine dicarboxamide series on the terminal sequence of {XpYp} telomere in {HT1080} cells.},\n\tvolume = {94},\n\tcopyright = {All rights reserved},\n\tissn = {1638-6183},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/22796264},\n\tdoi = {10.1016/j.biochi.2012.07.003},\n\tabstract = {Non-canonical four-stranded structures called G-quadruplexes can form among telomere repeats during its replication. Small molecule ligands able to interact and to stabilize G-quadruplexes were shown to disrupt the binding of essential telomeric components, such as POT1 and to trigger a telomeric dysfunction associated with a delayed growth arrest in tumor cells. We describe here the chemical synthesis and the G-quadruplex binding properties of three halogenated analogs of the 360A ligand that belongs to the 2,6 pyridine dicarboxamide series. 360A is now commonly used as a benchmark both for biophysical and cellular assays as this compound was shown to display a potent affinity and selectivity for telomeric G-quadruplex DNA over duplex DNA and to induce delayed growth inhibition in HT1080 tumor cell line. Two biophysical assays indicate that, in most cases, the presence of the halogen atom seems to slightly improve the interaction with the telomeric quadruplex. For stability reasons, the bromo derivative (360A-Br) was selected for the cellular assays. Since POT1 participates to the fine tuning of the C-strand end resection during telomere replication, we investigated the effect of 360A-Br to alter the terminal nucleotide composition of XpYp telomere in HT1080 cells using C-STELA. HT1080 cells treated for up to 24 days with 360A-Br presented some minor but significant variations of C-strand terminal nucleotide composition, also observed with a partial siRNA depletion of POT1. The relevance of these minor modifications of the telomeric C-strand resection induced by 360A-Br in HT1080 cells are discussed.},\n\tnumber = {12},\n\tjournal = {Biochimie},\n\tauthor = {Sidibe, Assitan and Hamon, Florian and Largy, Eric and Gomez, Dennis and Teulade-Fichou, Marie-Paule and Trentesaux, Chantal and Riou, Jean-François},\n\tmonth = jul,\n\tyear = {2012},\n\tpmid = {22796264},\n\tpages = {1--10},\n}\n\n
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\n Non-canonical four-stranded structures called G-quadruplexes can form among telomere repeats during its replication. Small molecule ligands able to interact and to stabilize G-quadruplexes were shown to disrupt the binding of essential telomeric components, such as POT1 and to trigger a telomeric dysfunction associated with a delayed growth arrest in tumor cells. We describe here the chemical synthesis and the G-quadruplex binding properties of three halogenated analogs of the 360A ligand that belongs to the 2,6 pyridine dicarboxamide series. 360A is now commonly used as a benchmark both for biophysical and cellular assays as this compound was shown to display a potent affinity and selectivity for telomeric G-quadruplex DNA over duplex DNA and to induce delayed growth inhibition in HT1080 tumor cell line. Two biophysical assays indicate that, in most cases, the presence of the halogen atom seems to slightly improve the interaction with the telomeric quadruplex. For stability reasons, the bromo derivative (360A-Br) was selected for the cellular assays. Since POT1 participates to the fine tuning of the C-strand end resection during telomere replication, we investigated the effect of 360A-Br to alter the terminal nucleotide composition of XpYp telomere in HT1080 cells using C-STELA. HT1080 cells treated for up to 24 days with 360A-Br presented some minor but significant variations of C-strand terminal nucleotide composition, also observed with a partial siRNA depletion of POT1. The relevance of these minor modifications of the telomeric C-strand resection induced by 360A-Br in HT1080 cells are discussed.\n
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