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\n  \n 2023\n \n \n (1)\n \n \n
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\n \n\n \n \n \n \n \n Mass spectrometry DDA parameters and global coverage of the metabolome: Spectral molecular networks of Momordica cardiospermoides plants.\n \n \n \n\n\n \n Ramabulana, A.; Petras, D.; Madala, N. E.; and Tugizimana, F.\n\n\n \n\n\n\n Metabolomics, 19(3): 1–13. 2023.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
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@article{Ramabulana2023,\nauthor = {Ramabulana, Anza-Tshilidzi and Petras, Daniel and Madala, Ntakadzeni E. and Tugizimana, Fidele},\ndoi = {10.1007/s11306-023-01981-4},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ramabulana et al. - 2023 - Mass spectrometry DDA parameters and global coverage of the metabolome Spectral molecular networks of Momordi.pdf:pdf},\nissn = {15733890},\njournal = {Metabolomics},\nkeywords = {data-dependent acquisition,molecular networking,momordica cardiospermoides,ms,ms parameter optimisation,ms spectra,natural products},\nnumber = {3},\npages = {1--13},\npmid = {36920561},\ntitle = {{Mass spectrometry DDA parameters and global coverage of the metabolome: Spectral molecular networks of Momordica cardiospermoides plants}},\nvolume = {19},\nyear = {2023}\n}\n
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\n  \n 2022\n \n \n (2)\n \n \n
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\n \n\n \n \n \n \n \n \n Identification of putative acyltransferase genes responsible for the biosynthesis of homogenous and heterogenous hydroxycinnamoyl-tartaric acid esters from Bidens pilosa.\n \n \n \n \n\n\n \n Mathatha, K.; Khwathisi, A.; Ramabulana, A. T.; Mwaba, I.; Mathomu, L. M.; and Madala, N. E.\n\n\n \n\n\n\n South African Journal of Botany, 149: 389–396. 2022.\n \n\n\n\n
\n\n\n\n \n \n \"IdentificationPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n 
@article{Mathatha2022,\nabstract = {Bidens pilosa is an edible plant with highly sought-after nutraceutical properties. The purported bioactivities of this plant can be correlated to the high number of metabolites. Amongst these metabolites, different derivatives of hydroxy‑cinnamoyl esters have been shown to exist in high proportions. However, the enzymatic machinery, thus the biosynthetic pathways responsible for the accumulation of these compounds in the plant have not yet been identified. For the first time, we report the putative identification of two genes with sequence homology to hydroxycinnamoyl-CoA: tartaric acid hydroxycinnamoyl transferase (HTT) in B. pilosa. The full-length sequence of the two isoforms of the HTT gene was achieved using single-molecule real-time (SMRT) sequencing approach. Analyses of methanolic extracts of B. pilosa through Liquid-chromatography hyphenated with mass spectrometry (LC-MS) technique revealed the existence of heterogeneous hydroxycinnamoyl-tartaric acid esters consisting of different hydroxycinnamoyl derivatives. To the best of our knowledge, this is a first report on these molecules from B. pilosa. Taken altogether, this plant utilises hydroxycinnamoyl-CoA tartaric hydroxycinnamoyl transferase (HTT) genes to diversify its metabolite composition through esterification of tartaric acid acceptor molecule by acylating it with either homogeneous or heterogenous hydroxycinnamic acids (HCA) derivatives. Therefore, B. pilosa is a source of structurally diverse isomeric compounds with purported nutraceutical values. The enzyme products of the two identified HTT genes are therefore pointed out as possible catalysts which can be further exploited by incorporating them in other economically viable plants to enhance the nutraceutical values thereof.},\nauthor = {Mathatha, Khuliso and Khwathisi, Adivhaho and Ramabulana, Anza Tshilidzi and Mwaba, Imah and Mathomu, Lutendo Michael and Madala, Ntakadzeni Edwin},\ndoi = {10.1016/j.sajb.2022.06.008},\nfile = {:C\\:/Users/ramab/Downloads/1-s2.0-S0254629922002976-main.pdf:pdf},\nissn = {02546299},\njournal = {South African Journal of Botany},\nkeywords = {Bidens pilosa,Hydroxycinnamic acids,Hydroxycinnamoyl-CoA: tartaric acid hydroxycinnamoyl transferase gene,Liquid chromatography mass spectrometry,Single molecule real time sequencing},\npages = {389--396},\npublisher = {Elsevier B.V.},\ntitle = {{Identification of putative acyltransferase genes responsible for the biosynthesis of homogenous and heterogenous hydroxycinnamoyl-tartaric acid esters from Bidens pilosa}},\nurl = {https://doi.org/10.1016/j.sajb.2022.06.008},\nvolume = {149},\nyear = {2022}\n}\n
\n
\n\n\n
\n Bidens pilosa is an edible plant with highly sought-after nutraceutical properties. The purported bioactivities of this plant can be correlated to the high number of metabolites. Amongst these metabolites, different derivatives of hydroxy‑cinnamoyl esters have been shown to exist in high proportions. However, the enzymatic machinery, thus the biosynthetic pathways responsible for the accumulation of these compounds in the plant have not yet been identified. For the first time, we report the putative identification of two genes with sequence homology to hydroxycinnamoyl-CoA: tartaric acid hydroxycinnamoyl transferase (HTT) in B. pilosa. The full-length sequence of the two isoforms of the HTT gene was achieved using single-molecule real-time (SMRT) sequencing approach. Analyses of methanolic extracts of B. pilosa through Liquid-chromatography hyphenated with mass spectrometry (LC-MS) technique revealed the existence of heterogeneous hydroxycinnamoyl-tartaric acid esters consisting of different hydroxycinnamoyl derivatives. To the best of our knowledge, this is a first report on these molecules from B. pilosa. Taken altogether, this plant utilises hydroxycinnamoyl-CoA tartaric hydroxycinnamoyl transferase (HTT) genes to diversify its metabolite composition through esterification of tartaric acid acceptor molecule by acylating it with either homogeneous or heterogenous hydroxycinnamic acids (HCA) derivatives. Therefore, B. pilosa is a source of structurally diverse isomeric compounds with purported nutraceutical values. The enzyme products of the two identified HTT genes are therefore pointed out as possible catalysts which can be further exploited by incorporating them in other economically viable plants to enhance the nutraceutical values thereof.\n
\n\n\n
\n\n\n
\n \n\n \n \n \n \n \n \n Effect of UV-induced geometrical isomerization of hydroxyl-cinnamic acid-containing molecules of bush tea (Athrixia phylicoides DC.) using UHPLC-QTOF-MS.\n \n \n \n \n\n\n \n Ramphinwa, M. L.; Madala, N. E.; Mchau, G. R.; Ramabulana, A. T.; and Mudau, F. N.\n\n\n \n\n\n\n Scientia Horticulturae, 301(April): 111124. 2022.\n \n\n\n\n
\n\n\n\n \n \n \"EffectPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n  \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n 
@article{Ramphinwa2022,\nabstract = {Bush tea (Athrixia phyllicoides DC.) is a herbal tea which contains bioactive compounds. Naturally, these metabolites aid plants to defend themselves against a wide spectrum of biotic and abiotic stresses. The objective of the study was to identify hydroxyl-cinnamic acids affected by UV (Ultraviolet) light exposure of bush tea through ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). A randomized complete block experimental design was used consisting of control and 80% white shade net replicated three times and samples were analyzed in triplicates. The methanolic leaf extracts of bush tea were exposed to UV light at 254 nm for 24 h and thereafter metabolites were measured and annotated through UHPLC-QTOF-MS. Hydroxyl-cinnamic acids (HCAs) have been shown to undergo photo-isomerization during post UV light exposure, evidenced by the emergence of photo-isomers. The findings showed that hydroxyl-cinnamic acids containing molecules to undergo photo-chemical isomerization, characterized by formation of isomeric molecules with cis geometry. Metabolites which underwent photo isomerization were found to be structurally related as they formed a very tight molecular family when molecular networking algorithm was used.},\nauthor = {Ramphinwa, Maanea L. and Madala, Ntakadzeni E. and Mchau, Godwin R.A. and Ramabulana, Anza T. and Mudau, Fhatuwani N.},\ndoi = {10.1016/j.scienta.2022.111124},\nfile = {:C\\:/Users/ramab/Downloads/1-s2.0-S030442382200245X-main.pdf:pdf},\nissn = {03044238},\njournal = {Scientia Horticulturae},\nkeywords = {Athrixia phyllicoides,Hydroxyl-cinnamic acids,Isomerization,Molecular network,Ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry},\nnumber = {April},\npages = {111124},\npublisher = {Elsevier B.V.},\ntitle = {{Effect of UV-induced geometrical isomerization of hydroxyl-cinnamic acid-containing molecules of bush tea (Athrixia phylicoides DC.) using UHPLC-QTOF-MS}},\nurl = {https://doi.org/10.1016/j.scienta.2022.111124},\nvolume = {301},\nyear = {2022}\n}\n
\n
\n\n\n
\n Bush tea (Athrixia phyllicoides DC.) is a herbal tea which contains bioactive compounds. Naturally, these metabolites aid plants to defend themselves against a wide spectrum of biotic and abiotic stresses. The objective of the study was to identify hydroxyl-cinnamic acids affected by UV (Ultraviolet) light exposure of bush tea through ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). A randomized complete block experimental design was used consisting of control and 80% white shade net replicated three times and samples were analyzed in triplicates. The methanolic leaf extracts of bush tea were exposed to UV light at 254 nm for 24 h and thereafter metabolites were measured and annotated through UHPLC-QTOF-MS. Hydroxyl-cinnamic acids (HCAs) have been shown to undergo photo-isomerization during post UV light exposure, evidenced by the emergence of photo-isomers. The findings showed that hydroxyl-cinnamic acids containing molecules to undergo photo-chemical isomerization, characterized by formation of isomeric molecules with cis geometry. Metabolites which underwent photo isomerization were found to be structurally related as they formed a very tight molecular family when molecular networking algorithm was used.\n
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\n
\n  \n 2021\n \n \n (3)\n \n \n
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\n \n\n \n \n \n \n \n A metabolic choreography of maize plants treated with a humic substance-based biostimulant under normal and starved conditions.\n \n \n \n\n\n \n Othibeng, K.; Nephali, L.; Ramabulana, A. T.; Steenkamp, P.; Petras, D.; Kang, K. B.; Opperman, H.; Huyser, J.; and Tugizimana, F.\n\n\n \n\n\n\n Metabolites, 11(6). 2021.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n 
@article{Othibeng2021,\nabstract = {Humic substance (HS)-based biostimulants show potentials as sustainable strategies for improved crop development and stress resilience. However, cellular and molecular mechanisms governing the agronomically observed effects of HS on plants remain enigmatic. Here, we report a global metabolic reprogramming of maize leaves induced by a humic biostimulant under normal and nutrient starvation conditions. This reconfiguration of the maize metabolism spanned chemical constellations, as revealed by molecular networking approaches. Plant growth and development under normal conditions were characterized by key differential metabolic changes such as increased levels of amino acids, oxylipins and the tricarboxylic acid (TCA) intermediate, isocitric acid. Furthermore, under starvation, the humic biostimulant significantly impacted pathways that are involved in stress-alleviating mechanisms such as redox homeostasis, strengthening of the plant cell wall, osmoregulation, energy production and membrane remodelling. Thus, this study reveals that the humic biostimulant induces a remodelling of inter-compartmental metabolic networks in maize, subsequently readjusting the plant physiology towards growth promotion and stress alleviation. Such insights contribute to ongoing efforts in elucidating modes of action of biostimulants, generating fundamental scientific knowledge that is necessary for development of the biostimulant industry, for sustainable food security.},\nauthor = {Othibeng, Kgalaletso and Nephali, Lerato and Ramabulana, Anza Tshilidzi and Steenkamp, Paul and Petras, Daniel and Kang, Kyo Bin and Opperman, Hugo and Huyser, Johan and Tugizimana, Fidele},\ndoi = {10.3390/metabo11060403},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Othibeng et al. - 2021 - A metabolic choreography of maize plants treated with a humic substance-based biostimulant under normal and sta.pdf:pdf},\nissn = {22181989},\njournal = {Metabolites},\nkeywords = {Abiotic stresses,Biostimulants,Humic substances,Metabolomics,Molecular networking},\nnumber = {6},\ntitle = {{A metabolic choreography of maize plants treated with a humic substance-based biostimulant under normal and starved conditions}},\nvolume = {11},\nyear = {2021}\n}\n
\n
\n\n\n
\n Humic substance (HS)-based biostimulants show potentials as sustainable strategies for improved crop development and stress resilience. However, cellular and molecular mechanisms governing the agronomically observed effects of HS on plants remain enigmatic. Here, we report a global metabolic reprogramming of maize leaves induced by a humic biostimulant under normal and nutrient starvation conditions. This reconfiguration of the maize metabolism spanned chemical constellations, as revealed by molecular networking approaches. Plant growth and development under normal conditions were characterized by key differential metabolic changes such as increased levels of amino acids, oxylipins and the tricarboxylic acid (TCA) intermediate, isocitric acid. Furthermore, under starvation, the humic biostimulant significantly impacted pathways that are involved in stress-alleviating mechanisms such as redox homeostasis, strengthening of the plant cell wall, osmoregulation, energy production and membrane remodelling. Thus, this study reveals that the humic biostimulant induces a remodelling of inter-compartmental metabolic networks in maize, subsequently readjusting the plant physiology towards growth promotion and stress alleviation. Such insights contribute to ongoing efforts in elucidating modes of action of biostimulants, generating fundamental scientific knowledge that is necessary for development of the biostimulant industry, for sustainable food security.\n
\n\n\n
\n\n\n
\n \n\n \n \n \n \n \n Application of plant growth regulators modulates the profile of chlorogenic acids in cultured bidens pilosa cells.\n \n \n \n\n\n \n Ramabulana, A.; Steenkamp, P.; Madala, N.; and Dubery, I.\n\n\n \n\n\n\n Plants, 10(3): 1–13. 2021.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
\n 
@article{Ramabulana2021,\nabstract = {Plant cell culture offers an alternative to whole plants for the production of biologically important specialised metabolites. In cultured plant cells, manipulation by auxin and cytokinin plant growth regulators (PGRs) may lead to in vitro organogenesis and metabolome changes. In this study, six different combination ratios of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) were investigated with the aim to induce indirect organogenesis from Bidens pilosa callus and to investigate the associated induced changes in the metabolomes of these calli. Phenotypic appearance of the calli and total phenolic contents of hydromethanolic extracts indicated underlying biochemical differences that were investigated using untargeted metabolomics, based on ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC– qTOF–MS), combined with multivariate data analysis. The concentration and combination ratios of PGRs were shown to induce differential metabolic responses and, thus, distinct metabolomic profiles, dominated by chlorogenic acids consisting of caffeoyl-and feruloyl-derivatives of quinic acid. Although organogenesis was not achieved, the results demonstrate that exogenous application PGRs can be used to manipulate the metabolome of B. pilosa for in vitro production of specialised metabolites with purported pharmacological properties.},\nauthor = {Ramabulana, A.-T. and Steenkamp, P.A. and Madala, N.E. and Dubery, I.A.},\ndoi = {10.3390/plants10030437},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ramabulana et al. - 2021 - Application of plant growth regulators modulates the profile of chlorogenic acids in cultured bidens pilosa c.pdf:pdf},\njournal = {Plants},\nnumber = {3},\npages = {1--13},\ntitle = {{Application of plant growth regulators modulates the profile of chlorogenic acids in cultured bidens pilosa cells}},\nvolume = {10},\nyear = {2021}\n}\n
\n
\n\n\n
\n Plant cell culture offers an alternative to whole plants for the production of biologically important specialised metabolites. In cultured plant cells, manipulation by auxin and cytokinin plant growth regulators (PGRs) may lead to in vitro organogenesis and metabolome changes. In this study, six different combination ratios of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) were investigated with the aim to induce indirect organogenesis from Bidens pilosa callus and to investigate the associated induced changes in the metabolomes of these calli. Phenotypic appearance of the calli and total phenolic contents of hydromethanolic extracts indicated underlying biochemical differences that were investigated using untargeted metabolomics, based on ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC– qTOF–MS), combined with multivariate data analysis. The concentration and combination ratios of PGRs were shown to induce differential metabolic responses and, thus, distinct metabolomic profiles, dominated by chlorogenic acids consisting of caffeoyl-and feruloyl-derivatives of quinic acid. Although organogenesis was not achieved, the results demonstrate that exogenous application PGRs can be used to manipulate the metabolome of B. pilosa for in vitro production of specialised metabolites with purported pharmacological properties.\n
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\n \n\n \n \n \n \n \n Metabolomics and molecular networking to characterize the chemical space of four momordica plant species.\n \n \n \n\n\n \n Ramabulana, A. T.; Petras, D.; Madala, N. E; and Tugizimana, F.\n\n\n \n\n\n\n Metabolites, 11(11): 11–14. 2021.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n 
@article{Ramabulana2021a,\nabstract = {Momordica plant species (Cucurbitaceae), have been used for centuries in traditional medicine and for nutritional purposes. Plants from this family are thus claimed to be phytochemically rich, representing an inexhaustible source of natural products. However, the chemical space of these Momordica species has not yet been fully decoded, and due to the inherent complexity of plant metabolomes, the characterization of the Momordica phytochemistry remains challenging. Thus, in this study we propose the use of molecular networking to unravel the molecular families within the metabolomes of four Momordica species (M. cardiospermoides, M. balsamina, M. charantia and M. foetida) and highlight the relevance of molecular networking in exploring the chemotaxonomy of these plants. In silico annotation tools (Network Annotation Propagation and DEREPLICATOR) and an unsupervised substructure identification tool (MS2LDA) were also explored to complement the classical molecular networking output and integration using MolNetEnhancer within GNPS. This allowed for the visualisation of chemical classes and the variety of substructures within the molecular families. The use of computational tools in this study highlighted various classes of metabolites, such as a wide range of flavonoids, terpenoids and lipids. Herein, these species are revealed to be phytochemically rich plants consisting of many biologically active metabolites differentially distributed within the different species, with the metabolome of M. cardiospermoides dereplicated in this paper for the first time.},\nauthor = {Ramabulana, Anza Tshilidzi and Petras, Daniel and Madala, Ntakadzeni E and Tugizimana, Fidele},\ndoi = {10.3390/metabo11110763},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ramabulana et al. - 2021 - Metabolomics and molecular networking to characterize the chemical space of four momordica plant species(2).pdf:pdf},\nissn = {22181989},\njournal = {Metabolites},\nkeywords = {GNPS,LC-MS,Metabolomics,Molecular networking,Momordica,Natural products},\nnumber = {11},\npages = {11--14},\ntitle = {{Metabolomics and molecular networking to characterize the chemical space of four momordica plant species}},\nvolume = {11},\nyear = {2021}\n}\n
\n
\n\n\n
\n Momordica plant species (Cucurbitaceae), have been used for centuries in traditional medicine and for nutritional purposes. Plants from this family are thus claimed to be phytochemically rich, representing an inexhaustible source of natural products. However, the chemical space of these Momordica species has not yet been fully decoded, and due to the inherent complexity of plant metabolomes, the characterization of the Momordica phytochemistry remains challenging. Thus, in this study we propose the use of molecular networking to unravel the molecular families within the metabolomes of four Momordica species (M. cardiospermoides, M. balsamina, M. charantia and M. foetida) and highlight the relevance of molecular networking in exploring the chemotaxonomy of these plants. In silico annotation tools (Network Annotation Propagation and DEREPLICATOR) and an unsupervised substructure identification tool (MS2LDA) were also explored to complement the classical molecular networking output and integration using MolNetEnhancer within GNPS. This allowed for the visualisation of chemical classes and the variety of substructures within the molecular families. The use of computational tools in this study highlighted various classes of metabolites, such as a wide range of flavonoids, terpenoids and lipids. Herein, these species are revealed to be phytochemically rich plants consisting of many biologically active metabolites differentially distributed within the different species, with the metabolome of M. cardiospermoides dereplicated in this paper for the first time.\n
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\n  \n 2020\n \n \n (2)\n \n \n
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\n \n\n \n \n \n \n \n Profiling of altered metabolomic states in bidens pilosa leaves in response to treatment by methyl jasmonate and methyl salicylate.\n \n \n \n\n\n \n Ramabulana, A. T.; Steenkamp, P. A.; Madala, N. E.; and Dubery, I. A.\n\n\n \n\n\n\n Plants, 9(10): 1–15. 2020.\n \n\n\n\n
\n\n\n\n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\n\n\n
\n 
@article{Ramabulana2020,\nabstract = {Bidens pilosa (Asteraceae) is an edible medicinal plant with many bioactivities reported to have a health-beneficial role in controling various diseases. Though B. pilosa contain a diverse array of natural products, these are produced in relatively low concentrations. A possible way to enhance secondary metabolite production can be through the use of elicitors. Here, the effects of exogenous treatments with two signal molecules—methyl jasmonate (MeJA) and methyl salicylate (MeSA)—on the metabolomic profiles of B. pilosa leaves were investigated. Plants were treated with 0.5 mM of MeJA or MeSA and harvested at 12 h and 24 h. Metabolites were extracted with methanol and separated on an ultra-high performance liquid chromatography system hyphenated to quadrupole time-of-flight mass spectrometry detection. Data was subjected to multivariate statistical analysis and modeling for annotation of metabolites. Hydroxycinnamic acid (HCA) derivatives, such as caffeoylquinic acids (CQAs), tartaric acid esters (chicoric acid and caftaric acid), chalcones, and flavonoids were identified as differentially regulated. The altered metabolomes in response to MeSA and MeJA overlapped to a certain extent, suggestive of a cross-talk between signaling and metabolic pathway activation. Moreover, the perturbation of isomeric molecules, especially the cis geometrical isomers of HCA derivatives by both treatments, further point to the biological significance of these molecules during physiological responses to stress. The results highlight the possibility of using phytohormones to enhance the accumulation of bioactive secondary metabolites in this plant.},\nauthor = {Ramabulana, Anza Tshilidzi and Steenkamp, Paul A. and Madala, Ntakadzeni E. and Dubery, Ian A.},\ndoi = {10.3390/plants9101275},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ramabulana et al. - 2020 - Profiling of altered metabolomic states in bidens pilosa leaves in response to treatment by methyl jasmonate.pdf:pdf},\nissn = {22237747},\njournal = {Plants},\nkeywords = {Bidens pilosa,Caffeoylquinic acid,Caftaric acid,Chicoric acid,Chlorogenic acid,Flavonoids,Metabolomic profiling,Methyl jasmonate,Methyl salicylate,Phytohormones},\nnumber = {10},\npages = {1--15},\ntitle = {{Profiling of altered metabolomic states in bidens pilosa leaves in response to treatment by methyl jasmonate and methyl salicylate}},\nvolume = {9},\nyear = {2020}\n}\n
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\n Bidens pilosa (Asteraceae) is an edible medicinal plant with many bioactivities reported to have a health-beneficial role in controling various diseases. Though B. pilosa contain a diverse array of natural products, these are produced in relatively low concentrations. A possible way to enhance secondary metabolite production can be through the use of elicitors. Here, the effects of exogenous treatments with two signal molecules—methyl jasmonate (MeJA) and methyl salicylate (MeSA)—on the metabolomic profiles of B. pilosa leaves were investigated. Plants were treated with 0.5 mM of MeJA or MeSA and harvested at 12 h and 24 h. Metabolites were extracted with methanol and separated on an ultra-high performance liquid chromatography system hyphenated to quadrupole time-of-flight mass spectrometry detection. Data was subjected to multivariate statistical analysis and modeling for annotation of metabolites. Hydroxycinnamic acid (HCA) derivatives, such as caffeoylquinic acids (CQAs), tartaric acid esters (chicoric acid and caftaric acid), chalcones, and flavonoids were identified as differentially regulated. The altered metabolomes in response to MeSA and MeJA overlapped to a certain extent, suggestive of a cross-talk between signaling and metabolic pathway activation. Moreover, the perturbation of isomeric molecules, especially the cis geometrical isomers of HCA derivatives by both treatments, further point to the biological significance of these molecules during physiological responses to stress. The results highlight the possibility of using phytohormones to enhance the accumulation of bioactive secondary metabolites in this plant.\n
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\n \n\n \n \n \n \n \n Profiling of Chlorogenic Acids from Bidens pilosa and Differentiation of Closely Related Positional Isomers with the Aid of UHPLC-QTOF-MS / MS- Based In-Source Collision-Induced Dissociation.\n \n \n \n\n\n \n Ramabulana, A.; Steenkamp, P.; Madala, N.; and Dubery, I. A\n\n\n \n\n\n\n Metabolites, 10(5): e178. 2020.\n \n\n\n\n
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@article{Ramabulana2020a,\nauthor = {Ramabulana, Anza-Tshilidzi and Steenkamp, Paul and Madala, Ntakadzeni and Dubery, Ian A},\ndoi = {10.3390/metabo10050178},\nfile = {:C\\:/Users/ramab/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ramabulana et al. - 2020 - Profiling of Chlorogenic Acids from Bidens pilosa and Differentiation of Closely Related Positional Isomer(2).pdf:pdf},\njournal = {Metabolites},\nkeywords = {bidens pilosa,cell culture,chlorogenic acids,hydroxycinnamic acids,iscid,metabolomics,phytochemicals},\nnumber = {5},\npages = {e178},\ntitle = {{Profiling of Chlorogenic Acids from Bidens pilosa and Differentiation of Closely Related Positional Isomers with the Aid of UHPLC-QTOF-MS / MS- Based In-Source Collision-Induced Dissociation}},\nvolume = {10},\nyear = {2020}\n}\n
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