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\n \n 2019\n \n \n (3)\n \n \n

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\n \n\n \n \n \n \n \n \n Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia.\n \n \n \n \n\n\n \n Sazanova, A.; Safronova, V.; Kuznetsova, I.; Karlov, D.; Belimov, A.; Andronov, E.; Chirak, E.; Popova, J.; Verkhozina, A.; Willems, A.; and Tikhonovich, I.\n\n\n \n\n\n\n International journal of systematic and evolutionary microbiology, 69(9): 2687-2695. 2019.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Bosea$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 2 downloads\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Sazanova20192687,\r\nauthor={Sazanova, A.L. and Safronova, V.I. and Kuznetsova, I.G. and Karlov, D.S. and Belimov, A.A. and Andronov, E.E. and Chirak, E.R. and Popova, J.P. and Verkhozina, A.V. and Willems, A. and Tikhonovich, I.A.},\r\ntitle={Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia},\r\njournal={International journal of systematic and evolutionary microbiology},\r\nyear={2019},\r\nvolume={69},\r\nnumber={9},\r\npages={2687-2695},\r\ndoi={10.1099/ijsem.0.003509},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071784234&doi=10.1099%2fijsem.0.003509&partnerID=40&md5=acecc7276b18c2e0f20cedba9d1a0329},\r\naffiliation={All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Sh. Podbelskogo 3, St. Petersburg, 196608, Russian Federation; Kazan Institute of Biochemistry and Biophysics of the Russian Academy of Sciences (KIBB of RAS), Lobachevsky Str. 2/31, Kazan, 420111, Russian Federation; Siberian Institute of Plant Physiology and Biochemistry (SIPPB), Lermontova Str. 132, Irkutsk, 664033, Russian Federation; Ghent University, Department of Biochemistry and Microbiology, Faculty of Sciences, K.L. Ledeganckstraat 35, Ghent, 9000, Belgium; Saint Petersburg State University, Department of Genetics and Biotechnology, Universitetskaya Emb. 7/9, St. Petersburg, 199034, Russian Federation},\r\nabstract={Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379T, Bosea vaviloviae LMG 28367T, Bosea massiliensis LMG 26221T and Bosea lupini LMG 26383T (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680T showed the highest sequence similarity to the type strain B. lupini LMG 26383T (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379T (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367T (86.8 % for ITS, 90.4 % for the other genes). The most abundant fatty acid was C18 : 1ω7c (40.8 %). The whole genomes of strains RCAM04680T and RCAM04685 were identical (100 % average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680T and B. vaviloviae LMG 28367T. The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Boseacaraganae sp. nov. (type strain RCAM04680T (=LMG 31125T), is proposed.},\r\nauthor_keywords={Bosea caraganae sp. nov.;  relict legumes;  root nodule bacteria},\r\npublisher={NLM (Medline)},\r\nissn={14665034},\r\npubmed_id={31166161},\r\nlanguage={English},\r\nabbrev_source_title={Int. J. Syst. Evol. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Viability of the soddy–podzolic soil microbial community after 148–1250 kGy gamma irradiation.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Planetary and Space Science, 172: 8-13. 2019.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Viability$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n The genetic diversity of microsymbionts from thermopsis lanceolata growing in Mongolia.\n \n \n \n \n\n\n \n Karlov, D.; Sazanova, A.; Kuznetsova, I.; Safronova, V.; Tikhomirova, N.; Popova, Z.; Osledkin, Y.; Verkhozina, A.; and Belimov, A.\n\n\n \n\n\n\n Ecological Genetics, 17(1): 43-51. 2019.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Karlov201943,\r\nauthor={Karlov, D.S. and Sazanova, A.L. and Kuznetsova, I.G. and Safronova, V.I. and Tikhomirova, N.Y. and Popova, Zh.P. and Osledkin, Yu.S. and Verkhozina, A.V. and Belimov, A.A.},\r\ntitle={The genetic diversity of microsymbionts from thermopsis lanceolata growing in Mongolia},\r\njournal={Ecological Genetics},\r\nyear={2019},\r\nvolume={17},\r\nnumber={1},\r\npages={43-51},\r\ndoi={10.17816/ecogen17143-51},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064383008&doi=10.17816%2fecogen17143-51&partnerID=40&md5=3e69baf036f00e09c1ff61d36c22d23b},\r\naffiliation={All-Russia Research Institute for Agricultural Microbiology, Saint Petersburg, Russian Federation; Siberian Institute of Plant Physiology and Biochemistry (SIPPB), Irkutsk, Russian Federation},\r\nabstract={For the first time, bacteria were isolated and identified from the root nodules of a wild-growing medicinal legume plant Thermopsis lanceolata, originated from Mongolia. The taxonomic position of 14 isolates obtained was determined using of sequencing of the 16S rRNA (rrs) and atpD genes. It was shown a significant biodiversity of the isolates from T. lanceolata, which belonged to three genera of the order Rhizobiales: Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae) and Bosea (family Bradyrhizobiaceae). Six isolates belonged to the species Phyllobacterium zundukense and Phyllobacterium trifolii (100 и 99,9% rrs similarity with the type strains P. zundukense Tri-48 T and P. trifolii PETP02 T , respectivelly), three isolates were identified as Rhizobium anhuiense (99,8% rrs similarity with the type strain R. anhuiense CCBAU 23252 T ). Two slow-growing isolates of the genus Bosea Tla-534 and Tla-545 may potentially belong to new species, since their rrs-similarity to the closest type strains B. massiliensis LMG 26221 T , B. lathyri LMG 26379 T and B. vaviloviae Vaf18T was 98,5-99,0%. Non-rhizobial strains were not isolated. The isolation and future investigation of the rhizobial microsymbionts of the valuable medicinal legume Thermopsis lanceolata is one of the necessary prerequisites for its industrial cultivation. © 2019 Eco-Vector LLC. All rights reserved.},\r\nauthor_keywords={16S rRNA;  AtpD genes;  Medicinal legume plants;  Root nodule bacteria;  Thermopsis lanceolata},\r\npublisher={Eco-Vector LLC},\r\nissn={18110932},\r\nlanguage={Russian},\r\nabbrev_source_title={Ecol. Genet.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2018\n \n \n (6)\n \n \n

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\n \n\n \n \n \n \n \n \n Unknown Widespread Iron- and Sulfur-Oxidizing Bacteria beneath the East Antarctic Ice Sheet.\n \n \n \n \n\n\n \n Bulat, S.; Doronin, M.; Pavlov, G.; Karlov, D.; Marie, D.; and Petit, J.\n\n\n \n\n\n\n Paleontological Journal, 52(10): 1196-1203. 2018.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Unknown$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 3 downloads\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat20181196,\r\nauthor={Bulat, S.A. and Doronin, M.V. and Pavlov, G.P. and Karlov, D.S. and Marie, D. and Petit, J.-R.},\r\ntitle={Unknown Widespread Iron- and Sulfur-Oxidizing Bacteria beneath the East Antarctic Ice Sheet},\r\njournal={Paleontological Journal},\r\nyear={2018},\r\nvolume={52},\r\nnumber={10},\r\npages={1196-1203},\r\ndoi={10.1134/S0031030118100076},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059868598&doi=10.1134%2fS0031030118100076&partnerID=40&md5=d1b81ec6d75f08a111fa9667d78b64dc},\r\naffiliation={Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC “Kurchatov Institute”, Gatchina, Leningrad oblast  188300, Russian Federation; Institute of Physics and Technology, Ural Federal University, Yekaterinburg, 620002, Russian Federation; Biological Station CNRS, Roscoff, France; IGE, “CNRS-UGA”, Grenoble, France},\r\nabstract={Abstract: Comparative analysis of the Vostok ice core (Central East Antarctica; one horizon, three boreholes) and D10 ice core (shoreline nearby the French Dumont d’Urville station) has reliably revealed three phylotypes (species) of aerobic iron-oxidizing betaproteobacteria of the family Gallionellaceae (closely related at the genus level to Sideroxydans lithotrophicus and Ferriphaselus amnicola), one of which has been detected from both the Vostok (borehole 5G-3) and D10 cores. In addition, the phylotype related to sulfur-oxidizing bacteria Tumebacillus sp. has been detected from both the Vostok (borehole 5G-2) and D10 cores. The both ice cores are almost equal in age, about 20 000 years; however, they differ in origin: the ice from Dumont d’Urville is atmospheric, while that from Vostok is a lake ice. The ice samples greatly vary in the storage time before treatment in the laboratory (from 0.5 to 40 years) and in intervals between treatments (from 1 to 5 years). The drilling sites are more than 1000 km apart. No evident hydrological links (the transfer of water beneath the ice sheet) between the Lake Vostok and Dumont D’Urville station have been found. This coincidence can be explained by the fact that minerals from the bedrock under the glacier, containing ferrous iron and reduced sulfur compounds, as well as physical and chemical conditions in both sites, liquid fresh water at a temperature near the freezing point, are similar. These and other assumptions are considered in the present article. © 2018, Pleiades Publishing, Ltd.},\r\nauthor_keywords={Antarctica;  bedrock;  D10 ice core;  Ferriphaselus sp;  iron-oxidizing bacteria;  mineral inclusions;  shared findings;  Sideroxydans sp;  sulfur-oxidizing bacteria;  Tumebacillus sp;  Vostok ice core},\r\n}

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\n \n\n \n \n \n \n \n \n Survivability of soil and permafrost microbial communities after irradiation with accelerated electrons under simulated martian and open space conditions.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Geosciences (Switzerland), 8(8). 2018.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"Survivability$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n One of the prior current astrobiological tasks is revealing the limits of microbial resistance to extraterrestrial conditions. Much attention is paid to ionizing radiation, since it can prevent the preservation and spread of life outside the Earth. The aim of this research was to study the impact of accelerated electrons ( 1 MeV) as component of space radiation on microbial communities in their natural habitat—the arid soil and ancient permafrost, and also on the pure bacterial cultures that were isolated from these ecotopes. The irradiation was carried out at low pressure ( 0.01 Torr) and low temperature (–130°C) to simulate the conditions of Mars or outer space. High doses of 10 kGy and 100 kGy were used to assess the effect of dose accumulation in inactive and hypometabolic cells, depending on environmental conditions under long-term irradiation estimated on a geological time scale. It was shown that irradiation with accelerated electrons in the applied doses did not sterilize native samples from Earth extreme habitats. The data obtained suggests that viable Earth-like microorganisms can be preserved in the anabiotic state for at least 1.3 and 20 million years in the regolith of modern Mars in the shallow subsurface layer and at a 5 m depth, respectively. In addition, the results of the study indicate the possibility of maintaining terrestrial like life in the ice of Europa at a 10 cm depth for at least 170 years or for at least 400 thousand years in open space within meteorites. It is established that bacteria in natural habitat has a much higher resistance to in situ irradiation with accelerated electrons when compared to their stability in pure isolated cultures. Thanks to the protective properties of the heterophase environment and the interaction between microbial populations even radiosensitive microorganisms as members of the native microbial communities are able to withstand very high doses of ionizing radiation. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.\n

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\n \n\n \n \n \n \n \n \n Helium and Neon in the Accreted Ice of the Subglacial Antarctic Lake Vostok.\n \n \n \n \n\n\n \n Jean-Baptiste, P.; Fourré, E.; Petit, J.; Lipenkov, V.; Bulat, S.; Chetverikov, Y.; and Raynaud, D.\n\n\n \n\n\n\n Geophysical Research Letters, 45(10): 4927-4932. 2018.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Helium$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Jean-Baptiste20184927,\r\nauthor={Jean-Baptiste, P. and Fourré, E. and Petit, J.R. and Lipenkov, V. and Bulat, S. and Chetverikov, Y. and Raynaud, D.},\r\ntitle={Helium and Neon in the Accreted Ice of the Subglacial Antarctic Lake Vostok},\r\njournal={Geophysical Research Letters},\r\nyear={2018},\r\nvolume={45},\r\nnumber={10},\r\npages={4927-4932},\r\ndoi={10.1029/2018GL078068},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047741270&doi=10.1029%2f2018GL078068&partnerID=40&md5=d5af60010167f09a67b09dd7c900150d},\r\naffiliation={Laboratoire des Sciences du Climat et de l'Environnement, CEA-CNRS-UVSQ, Gif-sur-Yvette, France; Institut des Sciences de l'Environnement IGE, Université Grenoble Alpes, CNRS, IRD, Grenoble, France; Arctic and Antarctic Research Institute, St. Petersburg, Russian Federation; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Russian Federation; Institute of Physics and Technology, Ural Federal University, Ekaterinburg, Russian Federation},\r\nabstract={We analyzed helium and neon in 24 samples from between 3,607 and 3,767 m (i.e., down to 2 m above the lake-ice interface) of the accreted ice frozen to the ceiling of Lake Vostok. Within uncertainties, the neon budget of the lake is balanced, the neon supplied to the lake by the melting of glacier ice being compensated by the neon exported by lake ice. The helium concentration in the lake is about 12 times more than in the glacier ice, with a measured 3He/4He ratio of 0.12 ± 0.01 Ra. This shows that Lake Vostok's waters are enriched by a terrigenic helium source. The 3He/4He isotope ratio of this helium source was determined. Its radiogenic value (0.057 × Ra) is typical of an old continental province, ruling out any magmatic activity associated with the tectonic structure of the lake. It corresponds to a low geothermal heat flow estimated at 51 mW/m2. ©2018. American Geophysical Union. All Rights Reserved.},\r\n}

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\n \n\n \n \n \n \n \n \n Content and Isotope Ratios of Noble Gases in Congelation Ice of Lake Vostok.\n \n \n \n \n\n\n \n Chetverikov, Y.; Aruev, N.; Bulat, S.; Gruzdov, K.; Ezhov, V.; Jean-Baptiste, P.; Kamenskii, I.; Lipenkov, V.; Prasolov, E.; Solovei, V.; Tyukal’tsev, R.; and Fedichkin, I.\n\n\n \n\n\n\n Technical Physics, 63(5): 738-746. 2018.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Content$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Chetverikov2018738,\r\nauthor={Chetverikov, Y.O. and Aruev, N.N. and Bulat, S.A. and Gruzdov, K.A. and Ezhov, V.F. and Jean-Baptiste, P. and Kamenskii, I.L. and Lipenkov, V.Y. and Prasolov, E.M. and Solovei, V.A. and Tyukal’tsev, R.V. and Fedichkin, I.L.},\r\ntitle={Content and Isotope Ratios of Noble Gases in Congelation Ice of Lake Vostok},\r\njournal={Technical Physics},\r\nyear={2018},\r\nvolume={63},\r\nnumber={5},\r\npages={738-746},\r\ndoi={10.1134/S1063784218050055},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048258544&doi=10.1134%2fS1063784218050055&partnerID=40&md5=1e811f9b44532b27806f94c7c295f009},\r\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute, Gatchina, 188300, Russian Federation; Laboratoire des Sciences du Climat et l’Environnement (LSCE), CNRS Orme des Merisiers, Gif-sur-Yvette, 91191, France; Federal Service for Hydrometeorology and Environmental Monitoring, Arctic and Antarctic Research Institute, St. Petersburg, 199397, Russian Federation; Karpinskii Russian Geological Research Institute, St. Petersburg, 199106, Russian Federation; Ioffe Institute, Russian Academy of Sciences, St. Petersburg, 194021, Russian Federation; Kola Science Centre, Russian Academy of Sciences, Apatity, 184209, Russian Federation},\r\nabstract={Isotope ratios of noble gases (He, Ne, Ar) were studied in samples collected by degassing of cores of water frozen over a glacier of Lake Vostok. The gases were collected into glass retorts during three days of degassing of cores, which have just been extracted from the borehole. Within the error, the isotope 3He/4He ratios of 0.28 ± 0.08 RA (RA = 1.38 × 10–6 is the ratio for air) correspond to those from [1]. The 4He/20Ne and 40Ar/36Ar ratios (12.4 ± 4.6 RA and 1.0074 ± 0.0023 RA, respectively) exceed their contents in air (4He/20NeA = 0.29; 40Ar/36ArA = 298.6) and may indicate some contribution of terrigenous gas to the gaseous balance of the lake, as well as the high content of ancient ground waters in the lake. The 3He/4He ratio of 0.28 RA means low mantle 3He flux typical of continental platforms far from active rift zones. © 2018, Pleiades Publishing, Ltd.},\r\ncorrespondence_address1={Chetverikov, Y.O.; Konstantinov Petersburg Nuclear Physics Institute, National Research Center Kurchatov InstituteRussian Federation; email: yurka@lns.pnpi.spb.ru},\r\npublisher={Pleiades Publishing},\r\nissn={10637842},\r\nlanguage={English},\r\nabbrev_source_title={Tech. Phys.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Isolation and identification of root nodule bacteria from guar cyamopsis tetragonoloba (L.) Taub.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Sel'skokhozyaistvennaya Biologiya, 53(6): 1285-1293. 2018.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Isolation$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n Cyamopsis tetragonoloba (guar) belongs to the family Fabaceae and is one of the promising crops for cultivation in Russia. Beans contain a large number of protein and fatty oil content, green beans can serve as a valuable source of food and feeds (as seed flour and not ground granulated feeds), but the plant is more in demand as a source of guar gum, which is a polysaccharide formed by galactose and mannose (galactomannan) and is contained in the endosperm of the seeds of this plant. Guar gum is widely used in various industries: food, textile, cosmetic, oil and other. Guar comes from India, where approximately 80 % of the world's production of guar gum is obtained. However, due to high demand, the plant is cultivated throughout the world in areas with a suitable climate (the USA, Sudan, Kenya, Pakistan, Australia), including in the south of the Russian Federation. It is known that the productivity of leguminous crops depends not only on climatic conditions, but also on the effectiveness of symbiosis of plants with nodule bacteria (rhizobia), which is determined by the nitrogen-fixing activity and competitiveness of strains, as well as their complementarity to a particular variety. The use of rhizobia for inoculation of plants is especially important when they are introduced to new habitats, so knowledge of its microsymbionts is necessary for successful cultivation of guar in Russia. This paper is the first to report on isolation of the nodule bacteria of the species Bradyrhizobium elkanii from root nodules of the guar plants grown in a pot experiment with the use of soil samples from India. We determined the taxonomic position and genetic heterogeneity of the isolated strains. The 16S rRNA gene (rrs), ITS-region between the 16S and 23S rDNA and three “housekeeping” genes atpD, dnaK and recA of 10 isolates of nodule bacteria were sequenced. According to the results of the rrs sequence analysis, all isolates are assigned to the species Bradyrhizobium elkanii (family Bradyrhizobiaceae), whose representatives are microsymbionts of a wide range of leguminous plants, including the tribe Indigofereae, to which the guar belongs. However, the representatives of the species were not previously described as a microsymbiont of Сyamopsis tetragonoloba. Sequencing of the ITS-region and the “housekeeping” genes confirmed the species identity of the isolates and demonstrated their genetic heterogeneity. Thus, the study of nodule bacteria from guar has expanded our knowledge of the phylogeny of its microsymbionts and will allow us in the future to select the most effective strains that improve nitrogen nutrition and plant growth. Knowledge of the rhizobial microsymbionts of guar will help maximize the symbiotic potential of this agronomically valuable culture for its stable and highly productive cultivation. © 2018 Russian Academy of Agricultural Sciences. All rights reserved.\n

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\n \n\n \n \n \n \n \n \n The search for Life on Mars and in the Solar System - Strategies, Logistics and Infrastructures.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n 2018.\n cited By 0; Conference of 69th International Astronautical Congress: #InvolvingEveryone, IAC 2018 ; Conference Date: 1 October 2018 Through 5 October 2018; Conference Code:147415\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n 100 kGy gamma-affected microbial communities within the ancient Arctic permafrost under simulated Martian conditions.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Extremophiles, 21(6): 1057-1067. 2017.\n cited By 10\n\n\n\n
\n\n\n\n \n \n $\"100 kGy$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n Microbial communities within the water column of freshwater Lake Radok, East Antarctica: predominant 16S rDNA phylotypes and bacterial cultures.\n \n \n \n \n\n\n \n Karlov, D.; Marie, D.; Sumbatyan, D.; Chuvochina, M.; Kulichevskaya, I.; Alekhina, I.; and Bulat, S.\n\n\n \n\n\n\n Polar Biology, 40(4): 823-836. 2017.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"Microbial$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Karlov2017823,\r\nauthor={Karlov, D.S. and Marie, D. and Sumbatyan, D.A. and Chuvochina, M.S. and Kulichevskaya, I.S. and Alekhina, I.A. and Bulat, S.A.},\r\ntitle={Microbial communities within the water column of freshwater Lake Radok, East Antarctica: predominant 16S rDNA phylotypes and bacterial cultures},\r\njournal={Polar Biology},\r\nyear={2017},\r\nvolume={40},\r\nnumber={4},\r\npages={823-836},\r\ndoi={10.1007/s00300-016-2008-9},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84980028615&doi=10.1007%2fs00300-016-2008-9&partnerID=40&md5=31303d36eee297d065681cc97f6d7565},\r\naffiliation={National Research Centre “Kurchatov Institute” B.P. Konstantinov, Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; Station Biologique de Roscoff, Place Georges Teissier, Roscoff, France; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia; S.N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russian Federation; Arctic and Antarctic Research Institute, St. Petersburg, Russian Federation; Institute of Physics and Technology, Ural Federal University, Ekaterinburg, Russian Federation},\r\nabstract={Antarctic lake ecosystems provide a rare opportunity to study the evolution and adaptation of microorganisms to extreme conditions, as well as to discover new species useful for biotechnological applications. Four water samples were collected from various layers of the water column of freshwater Lake Radok in East Antarctica. Two regions (v3-v5 and v4-v8) of the 16S rRNA gene were amplified by PCR and sequenced. Twenty dominant phylotypes were detected representing five bacterial phyla (Actinobacteria, α, β and δ Proteobacteria, Bacteroidetes, Planctomycetes, OD1) and two eukaryotic divisions (stramenopiles and green algae). Of these, 16 phylotypes demonstrated ≤98 % identity to the nearest taxa in GenBank and were therefore classified as new unknown species. Seven phylotypes demonstrated ≤90 % identity and thus remained unidentified. Actinobacteria was the most abundant phylum with 157 clones (41 % of the total number) representing 5 phylotypes. A species complex (3 clades from acI-A subgroup) of Candidatus Planktophila limnetica was prevalent in all layers. Representatives of the OD1 phylum and δ-proteobacteria were discovered by sequencing of the v3-v5 region of 16S rRNA, while Planctomycetes, β-proteobacteria and mtDNA of stramenopiles were discovered by sequencing of the v4-v8 region. This highlights the necessity of sequencing at least two 16S rRNA gene regions to gain more data on microbial community characterization. In general, despite the uniformity in the physical and chemical properties in the water column, a prominent stratification of microbial groups was observed, at the levels of both divisions and phylotypes. © 2016, Springer-Verlag Berlin Heidelberg.},\r\nauthor_keywords={16S rRNA;  Antarctica;  Freshwater lakes;  Lake Radok;  Microbial community},\r\ncorrespondence_address1={Karlov, D.S.; National Research Centre “Kurchatov Institute” B.P. Konstantinov, Petersburg Nuclear Physics InstituteRussian Federation; email: makondo07@gmail.com},\r\npublisher={Springer Verlag},\r\nissn={07224060},\r\ncoden={POBID},\r\nlanguage={English},\r\nabbrev_source_title={Polar Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2016\n \n \n (2)\n \n \n

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\n \n\n \n \n \n \n \n \n Technology of nondestructive light gas extraction from ice tested on samples from a bore hole above Vostok Lake.\n \n \n \n \n\n\n \n Chetverikov, Y.; Aruev, N.; Bulat, S.; Ezhov, V.; Lipenkov, V.; Solovei, V.; Tyukal’tsev, R.; and Fedichkin, I.\n\n\n \n\n\n\n Technical Physics, 61(7): 1091-1096. 2016.\n cited By 1\n\n\n\n
\n\n\n\n \n \n $\"Technology$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Chetverikov20161091,\r\nauthor={Chetverikov, Y.O. and Aruev, N.N. and Bulat, S.A. and Ezhov, V.F. and Lipenkov, V.Y. and Solovei, V.A. and Tyukal’tsev, R.V. and Fedichkin, I.L.},\r\ntitle={Technology of nondestructive light gas extraction from ice tested on samples from a bore hole above Vostok Lake},\r\njournal={Technical Physics},\r\nyear={2016},\r\nvolume={61},\r\nnumber={7},\r\npages={1091-1096},\r\ndoi={10.1134/S1063784216070082},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979708485&doi=10.1134%2fS1063784216070082&partnerID=40&md5=0140a0f6edb47ff8c736a282c4b22def},\r\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation; Ioffe Physical-Technical Institute, Russian Academy of Sciences, Politekhnicheskaya ul. 26, St. Petersburg, 194021, Russian Federation; Arctic and Antarctic Research Institute, ul. Beringa 38, St. Petersburg, 199397, Russian Federation; St. Petersburg State University, Universitetskaya nab. 7-9, St. Petersburg, 199034, Russian Federation},\r\nabstract={Nondestructive technology has been developed for the extraction of light gases dissolved in ice. The technology has been tested on samples of atmospheric and congealed ice of the 5-G3 bore hole of the Vostok station (East Antarctica) extracted from depths of 3457–3698 m. Down to 3539 m, ice is of an atmospheric origin, while ice deposited deeper is formed by natural water of Vostok Lake frozen on the glacier. Light gases were extracted into samplers (glass flasks) in the course of the 3-day degassing of samples freshly elevated from a bore hole. The samples were analyzed on the FT-1 time-of-flight mass spectrometer 6 months after sampling. Measurements reveal the presence of amounts of helium as well as molecular hydrogen considerably exceeding the atmospheric values. Measured values of gas ratio H2/4He = 5.4 ± 1.9 in the samples from depths of 3596–3698 m exceed the atmospheric values by more than an order of magnitude. © 2016, Pleiades Publishing, Ltd.},\r\ncorrespondence_address1={Chetverikov, Y.O.; Petersburg Nuclear Physics InstituteRussian Federation; email: yurka@lns.pnpi.spb.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={10637842},\r\nlanguage={English},\r\nabbrev_source_title={Tech. Phys.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Microbiology of the subglacial Lake Vostok: First results of borehole-frozen lake water analysis and prospects for searching for lake inhabitants.\n \n \n \n \n\n\n \n Bulat, S.\n\n\n \n\n\n\n Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 374(2059). 2016.\n cited By 13\n\n\n\n
\n\n\n\n \n \n $\"Microbiology$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat2016,\r\nauthor={Bulat, S.A.},\r\ntitle={Microbiology of the subglacial Lake Vostok: First results of borehole-frozen lake water analysis and prospects for searching for lake inhabitants},\r\njournal={Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences},\r\nyear={2016},\r\nvolume={374},\r\nnumber={2059},\r\ndoi={10.1098/rsta.2014.0292},\r\nart_number={20140292},\r\nnote={cited By 13},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956653436&doi=10.1098%2frsta.2014.0292&partnerID=40&md5=2be7c72a47afe40a6e440944c7257a3f},\r\naffiliation={Division of Molecular and Radiation Biophysics, B.P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre 'Kurchatov Institute', Gatchina, 188300, Russian Federation; Department of Physical Methods and Devices for Quality Control, Institute of Physics and Technology, Ural Federal University, Ekaterinburg, 620002, Russian Federation},\r\nabstract={This article examines the question of the possible existence of microbial life inhabiting the subglacial Lake Vostok buried beneath a 4 km thick Antarctic ice sheet. It represents the results of analysis of the only available frozen lake water samples obtained upon the first lake entry and subsequent re-coring the water frozen within the borehole. For comparison, results obtained by earlier molecular microbiological studies of accretion ice are included in this study, with the focus on thermophiles and an unknown bacterial phylotype. A description of two Lake Vostok penetrations is presented for the first time from the point of view of possible clean water sampling. Finally, the results of current studies of Lake Vostok frozen water samples are presented, with the focus on the discovery of another unknown bacterial phylotype w123-10 distantly related to the above-mentioned unknown phylotype AF532061 detected in Vostok accretion ice, both successfully passing all possible controls for contamination. The use of clean-room facilities and the establishment of a contaminant library are considered to be prerequisites for research on microorganisms from Lake Vostok. It seems that not yet recorded microbial life could exist within the Lake Vostok water body. In conclusion, the prospects for searching for lake inhabitants are expressed with the intention to sample the lake water as cleanly as possible in order to make sure that further results will be robust. © 2015 The Author(s) Published by the Royal Society. All rights reserved.},\r\nauthor_keywords={Antarctica;  Borehole-frozen water;  Contamination;  Extremophiles;  Subglacial Lake Vostok;  Unknown bacteria},\r\ncorrespondence_address1={Bulat, S.A.; Division of Molecular and Radiation Biophysics, B.P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre 'Kurchatov Institute'Russian Federation; email: bulat@omrb.pnpi.spb.ru},\r\npublisher={Royal Society of London},\r\nissn={1364503X},\r\npubmed_id={26667905},\r\nlanguage={English},\r\nabbrev_source_title={Philos. Trans. R. Soc. A Math. Phys. Eng. Sci.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2014\n \n \n (3)\n \n \n

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\n \n\n \n \n \n \n \n \n Diffuse scattering in Ih ice.\n \n \n \n \n\n\n \n Wehinger, B.; Chernyshov, D.; Krisch, M.; Bulat, S.; Ezhov, V.; and Bosak, A.\n\n\n \n\n\n\n Journal of Physics Condensed Matter, 26(26). 2014.\n cited By 13\n\n\n\n
\n\n\n\n \n \n $\"Diffuse$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Wehinger2014,\r\nauthor={Wehinger, B. and Chernyshov, D. and Krisch, M. and Bulat, S. and Ezhov, V. and Bosak, A.},\r\ntitle={Diffuse scattering in Ih ice},\r\njournal={Journal of Physics Condensed Matter},\r\nyear={2014},\r\nvolume={26},\r\nnumber={26},\r\ndoi={10.1088/0953-8984/26/26/265401},\r\nart_number={265401},\r\nnote={cited By 13},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84902279872&doi=10.1088%2f0953-8984%2f26%2f26%2f265401&partnerID=40&md5=2b47f9445de8583edb0c110f42da245e},\r\naffiliation={ESRF - the European Synchrotron, CS40220, 38043 Grenoble Cedex 9, France; Swiss-Norwegian Beamlines, European Synchrotron, Grenoble, France; Petersburg Nuclear Physics Institute, NRC Kurchatov Institute, Gatchina-188300-Saint-Petersburg, Russian Federation},\r\nabstract={Single crystals of ice Ih, extracted from the subglacial Lake Vostok accretion ice layer (3621 m depth) were investigated by means of diffuse x-ray scattering and inelastic x-ray scattering. The diffuse scattering was identified as mainly inelastic and rationalized in the frame of ab initio calculations for the ordered ice XI approximant. Together with Monte-Carlo modelling, our data allowed reconsidering previously available neutron diffuse scattering data of heavy ice as the sum of thermal diffuse scattering and static disorder contribution. © 2014 IOP Publishing Ltd.},\r\nauthor_keywords={ab initio calculation;  diffuse x-ray scattering;  ice Ih;  inelastic x-ray scattering;  lattice dynamics;  Monte-Carlo modelling;  static disorder},\r\npublisher={Institute of Physics Publishing},\r\nissn={09538984},\r\ncoden={JCOME},\r\nlanguage={English},\r\nabbrev_source_title={J Phys Condens Matter},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Archaeal diversity in permafrost deposits of Bunger Hills Oasis and King George Island (Antarctica) according to the 16S rRNA gene sequencing.\n \n \n \n \n\n\n \n Karaevskaia, E.; Demchenko, L.; Demidov, N.; Rivkina, E.; Bulat, S.; and Gilichinskiĭ, D.\n\n\n \n\n\n\n Mikrobiologiia, 83(4): 475-483. 2014.\n cited By 4\n\n\n\n
\n\n\n\n \n \n $\"Archaeal$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Karaevskaia2014475,\r\nauthor={Karaevskaia, E.S. and Demchenko, L.S. and Demidov, N.É and Rivkina, E.M. and Bulat, S.A. and Gilichinskiĭ, D.A.},\r\ntitle={Archaeal diversity in permafrost deposits of Bunger Hills Oasis and King George Island (Antarctica) according to the 16S rRNA gene sequencing},\r\njournal={Mikrobiologiia},\r\nyear={2014},\r\nvolume={83},\r\nnumber={4},\r\npages={475-483},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927785504&partnerID=40&md5=7759e7ec10814e38de4f08da4db9cae5},\r\nabstract={Archaeal communities of permafrost deposits of King George Island and Bunger Hills Oasis (Antarctica) differing in the content of biogenic methane were analyzed using clone libraries of two 16S rRNA gene regions. Phylotypes belonging to methanogenic archaea were identified in all horizons.},\r\nissn={00263656},\r\npubmed_id={25844459},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikrobiologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Archaeal diversity in permafrost deposits of Bunger Hills Oasis and King George Island (Antarctica) according to the 16S rRNA gene sequencing.\n \n \n \n \n\n\n \n Karaevskaya, E.; Demchenko, L.; Demidov, N.; Rivkina, E.; Bulat, S.; and Gilichinsky, D.\n\n\n \n\n\n\n Microbiology (Russian Federation), 83(4): 398-406. 2014.\n cited By 1\n\n\n\n
\n\n\n\n \n \n $\"Archaeal$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Karaevskaya2014398,\r\nauthor={Karaevskaya, E.S. and Demchenko, L.S. and Demidov, N.E. and Rivkina, E.M. and Bulat, S.A. and Gilichinsky, D.A.},\r\ntitle={Archaeal diversity in permafrost deposits of Bunger Hills Oasis and King George Island (Antarctica) according to the 16S rRNA gene sequencing},\r\njournal={Microbiology (Russian Federation)},\r\nyear={2014},\r\nvolume={83},\r\nnumber={4},\r\npages={398-406},\r\ndoi={10.1134/S0026261714040092},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927610605&doi=10.1134%2fS0026261714040092&partnerID=40&md5=0c001f53732bde73d2b3ab65e796e96e},\r\naffiliation={Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences, Pushchino, Russian Federation; Konstantinov Petersburg Nuclear Physics Institute, NRC “Kurchatov Institute”, Gatchina, Russian Federation},\r\nabstract={Archaeal communities of permafrost deposits of King George Island and Bunger Hills Oasis (Antarctica) differing in the content of biogenic methane were analyzed using the clone libraries of two 16S rRNA gene regions. Phylotypes belonging to methanogenic archaea were identified in all horizons. © 2014, Pleiades Publishing, Ltd.},\r\nauthor_keywords={Antarctica;  archaea;  biogenic methane;  permafrost},\r\ncorrespondence_address1={Karaevskaya, E.S.; Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of SciencesRussian Federation},\r\npublisher={Maik Nauka Publishing / Springer SBM},\r\nissn={00262617},\r\nlanguage={English},\r\nabbrev_source_title={Microbiology},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Vostok Subglacial Lake: Details of Russian Plans/Activities for Drilling and Sampling.\n \n \n \n \n\n\n \n Lukin, V.; and Bulat, S.\n\n\n \n\n\n\n Wiley Blackwell, 2013.\n cited By 7\n\n\n\n
\n\n\n\n \n \n $\"Vostok$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@BOOK{Lukin2013187,\r\nauthor={Lukin, V. and Bulat, S.},\r\ntitle={Vostok Subglacial Lake: Details of Russian Plans/Activities for Drilling and Sampling},\r\njournal={Antarctic Subglacial Aquatic Environments},\r\nyear={2013},\r\npages={187-197},\r\ndoi={10.1002/9781118670354.ch11},\r\nnote={cited By 7},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84951267754&doi=10.1002%2f9781118670354.ch11&partnerID=40&md5=2f237b74107d9e85b9aa3621b46bd3ce},\r\naffiliation={Russian Antarctic Expedition, Arctic and Antarctic Research Institute, 38 Bering str., St. Petersburg, 199397, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Leningrad region, Gatchina, 188300, Russian Federation},\r\nabstract={The Russian Federation has developed a national project involving the drilling and sampling of Vostok Subglacial Lake, East Antarctica. The objective is to explore this extreme icy environment, using a variety of techniques to identify the forms and levels of life that exist there. The project is funded by the Russian Federal Service ROSHYDROMET. In the 2009/2010 season, drilling operations were restarted at a depth of 3559 m via new borehole 5G-2, successfully reaching a new depth of approximately 3650 m. New accretion ice, including the inclusion-rich "thermophile-containing" horizon (around 3608 m) was again recovered and will be studied to assess the previous scenario and findings. In 2010/2011, the drill will carefully continue to deepen the borehole leaving a 10- to 15-m ice cork and will in season 2011/2012 enter the lake, allowing water to rise up dozens of meters within borehole 5G-2 and subsequently freeze. During the same or following season (2012/2013), borehole 5G-2 will be redrilled to acquire rapidly frozen lake water for complex investigations. In the following season, 2013/2014, a special set of strictly decontaminated biophysical instruments, developed at the Petersburg Nuclear Physics Institute, will be lowered into the water body, with a battery of ocean observatory sensors, cameras, fluorimeters-spectrometers, and special water samplers on board several submersible titan modules. Such activities are in line with environmental stewardship in the exploration of unique aquatic environments under the Antarctic ice sheet. © 2011 by the American Geophysical Union. All rights reserved.},\r\nauthor_keywords={Antarctica;  Aquatic ecology-Antarctica;  Biogeochemistry;  Deepicedrilling;  Extremophiles;  Life;  Subglacial lakes-Antarctica-Discovery and exploration;  Subglacial lakes-Antarctica-History;  Subglacial lakes-Polar regions-Discovery and exploration;  Subglacial lakes-Polar regions-History;  VostokSubglacialLake},\r\ncorrespondence_address1={Lukin, V.; Russian Antarctic Expedition, Arctic and Antarctic Research Institute, 38 Bering str., Russian Federation; email: lukin@aari.nw.ru},\r\npublisher={Wiley Blackwell},\r\nisbn={9781118670354; 9780875904825},\r\nlanguage={English},\r\nabbrev_source_title={Antarct. Subglacial Aquat. Environ.},\r\ndocument_type={Book Chapter},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2011\n \n \n (8)\n \n \n

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\n \n\n \n \n \n \n \n \n Community variability of bacteria in alpine snow (Mont Blanc) containing Saharan dust deposition and their snow colonisation potential.\n \n \n \n \n\n\n \n Chuvochina, M.; Marie, D.; Chevaillier, S.; Petit, J.; Normand, P.; Alekhina, I.; and Bulat, S.\n\n\n \n\n\n\n Microbes and Environments, 26(3): 237-247. 2011.\n cited By 26\n\n\n\n
\n\n\n\n \n \n $\"Community$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Chuvochina2011237,\r\nauthor={Chuvochina, M.S. and Marie, D. and Chevaillier, S. and Petit, J.-R. and Normand, P. and Alekhina, I.A. and Bulat, S.A.},\r\ntitle={Community variability of bacteria in alpine snow (Mont Blanc) containing Saharan dust deposition and their snow colonisation potential},\r\njournal={Microbes and Environments},\r\nyear={2011},\r\nvolume={26},\r\nnumber={3},\r\npages={237-247},\r\ndoi={10.1264/jsme2.ME11116},\r\nnote={cited By 26},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052460023&doi=10.1264%2fjsme2.ME11116&partnerID=40&md5=f07404d66556d07e59d29f1c2c84d0b7},\r\naffiliation={Laboratoire de Glaciologie et de Geophysique de l'Environnement, LGGE CNRS, Universite Joseph Fourier, BP 96, 38402 Saint Martin d'Hères cedex, France; Eukaryote's Genetics Laboratory, Petersburg Nuclear Physics Institute Russian Academy of Sciences, Orlova Rozcha 1, Leningrad Region, Gatchina, 188300, Russian Federation; Equipe procaryotes photosynthetiques marins, UMR CNRS 7144, Station Biologique de Roscoff, 29682 Roscoff, France; LISA, UMR CNRS 7583, Universite Paris Est Creteil et Universite Paris Diderot, Institut Pierre Simon Laplace, Creteil, France; Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, Universite Lyon 1, Universite Lyon, 69622 Villeurbanne, France},\r\nabstract={Microorganisms uplifted during dust storms survive long-range transport in the atmosphere and could colonize high-altitude snow. Bacterial communities in alpine snow on a Mont Blanc glacier, associated with four depositions of Saharan dust during the period 2006-2009, were studied using 16S rRNA gene sequencing and flow cytometry. Also, sand from the Tunisian Sahara, Saharan dust collected in Grenoble and Mont Blanc snow containing no Saharan dust (one sample of each) were analyzed. The bacterial community composition varied significantly in snow containing four dust depositions over a 3-year period. Out of 61 phylotypes recovered from dusty snow, only three phylotypes were detected in more than one sample. Overall, 15 phylotypes were recognized as potential snow colonizers. For snow samples, these phylotypes belonged to Actinobacteria, Proteobacteria and Cyanobacteria, while for Saharan sand/dust samples they belonged to Actinobacteria, Bacteroidetes, Deinococcus-Thermus and Proteobacteria. Thus, regardless of the time-scale, Saharan dust events can bring different microbiota with no common species set to alpine glaciers. This seems to be defined more by event peculiarities and aeolian transport conditions than by the bacterial load from the original dust source.},\r\nauthor_keywords={16S rRNA genes;  Bacterial community composition;  Mont blanc glacier;  Saharan dust;  Snow},\r\ncorrespondence_address1={Chuvochina, M. S.; Laboratoire de Glaciologie et de Geophysique de l'Environnement, LGGE CNRS, Universite Joseph Fourier, BP 96, 38402 Saint Martin d'Hères cedex, France; email: chuvochina@omrb.pnpi.spb.ru},\r\nissn={13426311},\r\npubmed_id={21666389},\r\nlanguage={English},\r\nabbrev_source_title={Microbes Environ.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Searching for life in extreme environments relevant to Jovian's Europa: Lessons from subglacial ice studies at Lake Vostok (East Antarctica).\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Advances in Space Research, 48(4): 697-701. 2011.\n cited By 9\n\n\n\n
\n\n\n\n \n \n $\"Searching$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n The objective was to estimate the genuine microbial content of ice samples from refrozen water (accretion ice) from the subglacial Lake Vostok (Antarctica) buried beneath the 4-km thick East Antarctic ice sheet. The samples were extracted by heavy deep ice drilling from 3659 m below the surface. High pressure, a low carbon and chemical content, isolation, complete darkness and the probable excess of oxygen in water for millions of years characterize this extreme environment. A decontamination protocol was first applied to samples selected for the absence of cracks to remove the outer part contaminated by handling and drilling fluid. Preliminary indications showed the accretion ice samples to be almost gas free with a low impurity content. Flow cytometry showed the very low unevenly distributed biomass while repeated microscopic observations were unsuccessful. We used strategies of Ancient DNA research that include establishing contaminant databases and criteria to validate the amplification results. To date, positive results that passed the artifacts and contaminant databases have been obtained for a pair of bacterial phylotypes only in accretion ice samples featured by some bedrock sediments. The phylotypes included the chemolithoautotrophic thermophile Hydrogenophilus thermoluteolus and one unclassified phylotype. Combined with geochemical and geophysical considerations, our results suggest the presence of a deep biosphere, possibly thriving within some active faults of the bedrock encircling the subglacial lake, where the temperature is as high as 50 °C and in situ hydrogen is probably present. Our approach indicates that the search for life in the subglacial Lake Vostok is constrained by a high probability of forward-contamination. Our strategy includes strict decontamination procedures, thorough tracking of contaminants at each step of the analysis and validation of the results along with geophysical and ecological considerations for the lake setting. This may serve to establish a guideline protocol for studying extraterrestrial ice samples. © 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.\n

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\n \n\n \n \n \n \n \n \n Analog environments for a Europa lander mission.\n \n \n \n \n\n\n \n Lorenz, R.; Gleeson, D.; Prieto-Ballesteros, O.; Gomez, F.; Hand, K.; and Bulat, S.\n\n\n \n\n\n\n Advances in Space Research, 48(4): 689-696. 2011.\n cited By 11\n\n\n\n
\n\n\n\n \n \n $\"Analog$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Lorenz2011689,\r\nauthor={Lorenz, R.D. and Gleeson, D. and Prieto-Ballesteros, O. and Gomez, F. and Hand, K. and Bulat, S.},\r\ntitle={Analog environments for a Europa lander mission},\r\njournal={Advances in Space Research},\r\nyear={2011},\r\nvolume={48},\r\nnumber={4},\r\npages={689-696},\r\ndoi={10.1016/j.asr.2010.05.006},\r\nnote={cited By 11},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960239971&doi=10.1016%2fj.asr.2010.05.006&partnerID=40&md5=c9a0225848e1cfae565f740cb6b09097},\r\naffiliation={JHU Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20723, United States; Department of Geological Sciences, University of Colorado, Boulder, CO 80309, United States; Centro de Astrobiologia (INTA-CSIC), Torrejon de Ardoz, 28850, Madrid, Spain; Jet Propulsion Laboratory, 4800 Oak Grove Drive, Pasadena, CA 91109, United States; Petersburg Nuclear Physics Institute, 188300 St. Petersburg-Gatchina, Russian Federation},\r\nabstract={This paper reviews the utility of analog environments in preparations for a Europa lander mission. Such analogs are useful in the demonstration and rehearsal of engineering functions such as sample acquisition from an icy surface, as well as in the exercise of the scientific protocols needed to identify organic, inorganic and possible biological impurities in ice. Particular attention is drawn to Antarctic and Arctic analog sites where progress in these latter areas has been significant in recent years. © 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.},\r\nauthor_keywords={Analog field studies;  Antarctica;  Astrobiology;  Europa;  Ice},\r\ncorrespondence_address1={Lorenz, R.D.; JHU Applied Physics Laboratory, 11100 Johns Hopkins Road, Laurel, MD 20723, United States; email: Ralph.lorenz@jhuapl.edu},\r\npublisher={Elsevier Ltd},\r\nissn={02731177},\r\ncoden={ASRSD},\r\nlanguage={English},\r\nabbrev_source_title={Adv. Space Res.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Microbial communities of water column of Lake Radok, East Antarctica, dominated by abundant actinobacterium \"Candidatus Planktophila limnetica\".\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Microbiology, 80(4): 576-579. 2011.\n cited By 7\n\n\n\n
\n\n\n\n \n \n $\"Microbial$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n [Microbial communities of water column of Lake Radok, East Antarctica, dominated by abundant actinobacterium \" Candidatus Planktophila limnetica\" ].\n \n \n \n \n\n\n \n Karlov, D.; Marie, D.; Chuvochina, M.; Alekhina, I.; and Bulat, S.\n\n\n \n\n\n\n Mikrobiologiia, 80(4): 571-574. 2011.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"[Microbial$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Karlov2011571,\r\nauthor={Karlov, D.S. and Marie, D. and Chuvochina, M.S. and Alekhina, I.A. and Bulat, S.A.},\r\ntitle={[Microbial communities of water column of Lake Radok, East Antarctica, dominated by abundant actinobacterium " Candidatus Planktophila limnetica" ].},\r\njournal={Mikrobiologiia},\r\nyear={2011},\r\nvolume={80},\r\nnumber={4},\r\npages={571-574},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856366821&partnerID=40&md5=c0b69309b12c12fba93311a4830dec15},\r\ncorrespondence_address1={Karlov, D.S.},\r\nissn={00263656},\r\npubmed_id={22073560},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikrobiologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Three events of Saharan dust deposition on the Mont Blanc glacier associated with different snow-colonizing bacterial phylotypes.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Microbiology, 80(1): 125-131. 2011.\n cited By 17\n\n\n\n
\n\n\n\n \n \n $\"Three$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n Quantification of dissolved organic carbon at very low levels in natural ice samples by a uv-induced oxidation method.\n \n \n \n \n\n\n \n Preunkert, S.; Legrand, M.; Stricker, P.; Bulat, S.; Alekhina, I.; Petit, J.; Hoffmann, H.; May, B.; and Jourdain, B.\n\n\n \n\n\n\n Environmental Science and Technology, 45(2): 673-678. 2011.\n cited By 17\n\n\n\n
\n\n\n\n \n \n $\"Quantification$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Preunkert2011673,\r\nauthor={Preunkert, S. and Legrand, M. and Stricker, P. and Bulat, S. and Alekhina, I. and Petit, J.R. and Hoffmann, H. and May, B. and Jourdain, B.},\r\ntitle={Quantification of dissolved organic carbon at very low levels in natural ice samples by a uv-induced oxidation method},\r\njournal={Environmental Science and Technology},\r\nyear={2011},\r\nvolume={45},\r\nnumber={2},\r\npages={673-678},\r\ndoi={10.1021/es1023256},\r\nnote={cited By 17},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-78651385545&doi=10.1021%2fes1023256&partnerID=40&md5=e2cda55a922b663229e0d33a6e935994},\r\naffiliation={Laboratoire de Glaciologie et Géophysique de LEnvironnement, Centre National de la Recherche Scientifique, St Martin dHéres, France; Institut fur Umweltphysik, Ruprecht Karls-Universitat Heidelberg, Heidelberg, Germany; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Leningrad Region, Gatchina, Russian Federation},\r\nabstract={The study of chemical impurities trapped in solid precipitation and accumulated in polar ice sheets and high-elevation, midlatitude cold glaciers over the last several hundreds of years provides a unique way to reconstruct our changing atmosphere from the preindustrial era to the present day. Numerous ice core studies of inorganic species have already evaluated the effects of growing anthropogenic emissions of SO2 or NOx on the chemical composition of the atmosphere in various regions of the world. While it was recently shown that organic species dominate the atmospheric aerosol mass, the contribution of anthropogenic emissions to their budget remains poorly understood. The study of organics in ice is at the infancy stage, and it still is difficult to draw a consistent picture of the organic content of polar ice from sparse available data. A UV oxidation method and IR quantification of CO2 was optimized to obtain measurements of dissolved organic carbon content as low as a few ppbC. Stringent working conditions were defined to prevent contamination during the cleaning of ice. Measurements in various ice cores corresponding to preindustrial times revealed dissolved organic carbon content of less than 10 ppbC in Antarctica and up to 75 ppbC in alpine ice. © 2011 American Chemical Society.},\r\ncorrespondence_address1={Preunkert, S.; Laboratoire de Glaciologie et Géophysique de LEnvironnement, Centre National de la Recherche Scientifique, St Martin dHéres, France; email: preunkert@lgge.obs.ujf-grenoble.fr},\r\nissn={0013936X},\r\ncoden={ESTHA},\r\npubmed_id={21142062},\r\nlanguage={English},\r\nabbrev_source_title={Environ. Sci. Technol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Vostok Subglacial Lake: Details of Russian plans/activities for drilling and sampling.\n \n \n \n \n\n\n \n Lukin, V.; and Bulat, S.\n\n\n \n\n\n\n Geophysical Monograph Series, 192: 187-197. 2011.\n cited By 13\n\n\n\n
\n\n\n\n \n \n $\"Vostok$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n \n \n 1 download\n \n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Lukin2011187,\r\nauthor={Lukin, V. and Bulat, S.},\r\ntitle={Vostok Subglacial Lake: Details of Russian plans/activities for drilling and sampling},\r\njournal={Geophysical Monograph Series},\r\nyear={2011},\r\nvolume={192},\r\npages={187-197},\r\ndoi={10.1029/2010GM000951},\r\nnote={cited By 13},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857098263&doi=10.1029%2f2010GM000951&partnerID=40&md5=a501fc0f5b4105be4c613fe8b66cb1f1},\r\naffiliation={Russian Antarctic Expedition, Arctic and Antarctic Research Institute, 38 Bering str., St.Petersburg 199397, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Leningrad region, Gatchina 188300, Russian Federation},\r\nabstract={The Russian Federation has developed a national project involving the drilling and sampling of Vostok Subglacial Lake, East Antarctica. The objective is to explore this extreme icy environment, using a variety of techniques to identify the forms and levels of life that exist there. The project is funded by the Russian Federal Service ROSHYDROMET. In the 2009/2010 season, drilling operations were restarted at a depth of 3559 m via new borehole 5G-2, successfully reaching a new depth of approximately 3650 m. New accretion ice, including the inclusion-rich "thermophile-containing" horizon (around 3608 m) was again recovered and will be studied to assess the previous scenario and findings. In 2010/2011, the drill will carefully continue to deepen the borehole leaving a 10- to 15-m ice cork and will in season 2011/2012 enter the lake, allowing water to rise up dozens of meters within borehole 5G-2 and subsequently freeze. During the same or following season (2012/2013), borehole 5G-2 will be redrilled to acquire rapidly frozen lake water for complex investigations. In the following season, 2013/2014, a special set of strictly decontaminated biophysical instruments, developed at the Petersburg Nuclear Physics Institute, will be lowered into the water body, with a battery of ocean observatory sensors, cameras, fluorimeters-spectrometers, and special water samplers on board several submersible titan modules. Such activities are in line with environmental stewardship in the exploration of unique aquatic environments under the Antarctic ice sheet. Copyright © 2010 by the American Geophysical Union.},\r\npublisher={American Geophysical Union},\r\nisbn={9780875904825},\r\nlanguage={English},\r\nabbrev_source_title={Geophys. Monogr. Ser.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2009\n \n \n (2)\n \n \n

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\n \n\n \n \n \n \n \n \n Cell concentrations of microorganisms in glacial and lake ice of the Vostok ice core, East Antarctica.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Microbiology, 78(6): 808-810. 2009.\n cited By 26\n\n\n\n
\n\n\n\n \n \n $\"Cell$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n Ultra-low rare earth element content in accreted ice from sub-glacial Lake Vostok, Antarctica.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Geochimica et Cosmochimica Acta, 73(20): 5959-5974. 2009.\n cited By 9\n\n\n\n
\n\n\n\n \n \n $\"Ultra-low$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n 2007\n \n \n (1)\n \n \n

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\n \n\n \n \n \n \n \n \n Molecular analysis of bacterial diversity in kerosene-based drilling fluid from the deep ice borehole at Vostok, East Antarctica.\n \n \n \n \n\n\n \n Alekhina, I.; Marie, D.; Petit, J.; Lukin, V.; Zubkov, V.; and Bulat, S.\n\n\n \n\n\n\n FEMS Microbiology Ecology, 59(2): 289-299. 2007.\n cited By 27\n\n\n\n
\n\n\n\n \n \n $\"Molecular$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Alekhina2007289,\r\nauthor={Alekhina, I.A. and Marie, D. and Petit, J.R. and Lukin, V.V. and Zubkov, V.M. and Bulat, S.A.},\r\ntitle={Molecular analysis of bacterial diversity in kerosene-based drilling fluid from the deep ice borehole at Vostok, East Antarctica},\r\njournal={FEMS Microbiology Ecology},\r\nyear={2007},\r\nvolume={59},\r\nnumber={2},\r\npages={289-299},\r\ndoi={10.1111/j.1574-6941.2006.00271.x},\r\nnote={cited By 27},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846265657&doi=10.1111%2fj.1574-6941.2006.00271.x&partnerID=40&md5=bcd7537b6d04b8f79e89f1dbaac98728},\r\naffiliation={Petersburg Nuclear Physics Institute RAS, St Petesrburg-Gatchina, Russian Federation; Laboratory of Glaciology and Geophysics of Environment CNRS, Grenoble, France; Station Biologique de Roscoff, France; Arctic and Antarctic Research Institute, St Petersburg, Russian Federation; St. Petersburg State Mining Institute, St Petersburg, Russian Federation; Petersburg Nuclear Physics Institute RAS, St Petersburg-Gatchina, 188300, Russian Federation},\r\nabstract={Decontamination of ice cores is a critical issue in phylogenetic studies of glacial ice and subglacial lakes. At the Vostok drill site, a total of 3650 m of ice core have now been obtained from the East Antarctic ice sheet. The ice core surface is coated with a hard-to-remove film of impure drilling fluid comprising a mixture of aliphatic and aromatic hydrocarbons and foranes. In the present study we used 16S rRNA gene sequencing to analyze the bacterial content of the Vostok drilling fluid sampled from four depths in the borehole. Six phylotypes were identified in three of four samples studied. The two dominant phylotypes recovered from the deepest (3400 and 3600 m) and comparatively warm (-10°C and -6°C, respectively) borehole horizons were from within the genus Sphingomonas, a well-known degrader of polyaromatic hydrocarbons. The remaining phylotypes encountered in all samples proved to be human- or soil-associated bacteria and were presumed to be drilling fluid contaminants of rare occurrence. The results obtained indicate the persistence of bacteria in extremely cold, hydrocarbon-rich environments. They show the potential for contamination of ice and subglacial water samples during lake exploration, and the need to develop a microbiological database of drilling fluid findings. © 2007 Federation of European Microbiological Societies.},\r\nauthor_keywords={Antarctica;  Contamination;  Drilling fluid;  Extremophiles;  Lake Vostok;  Sphingomonas},\r\ncorrespondence_address1={Alekhina, I.A.; Petersburg Nuclear Physics Institute RAS, St Petersburg-Gatchina, 188300, Russian Federation; email: alekhina@omrb.pnpi.spb.ru},\r\nissn={01686496},\r\ncoden={FMECE},\r\npubmed_id={17313578},\r\nlanguage={English},\r\nabbrev_source_title={FEMS Microbiol. Ecol.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox.\n \n \n \n \n\n\n \n Lavire, C.; Normand, P.; Alekhina, I.; Bulat, S.; Prieur, D.; Birrien, J.; Fournier, P.; Hänni, C.; and Petit, J.\n\n\n \n\n\n\n Environmental Microbiology, 8(12): 2106-2114. 2006.\n cited By 34\n\n\n\n
\n\n\n\n \n \n $\"Presence$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Lavire20062106,\r\nauthor={Lavire, C. and Normand, P. and Alekhina, I. and Bulat, S. and Prieur, D. and Birrien, J.-L. and Fournier, P. and Hänni, C. and Petit, J.-R.},\r\ntitle={Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox},\r\njournal={Environmental Microbiology},\r\nyear={2006},\r\nvolume={8},\r\nnumber={12},\r\npages={2106-2114},\r\ndoi={10.1111/j.1462-2920.2006.01087.x},\r\nnote={cited By 34},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750900944&doi=10.1111%2fj.1462-2920.2006.01087.x&partnerID=40&md5=ff94d1caad681a50c8eea0d74f0e26a8},\r\naffiliation={UMR-CNRS 5557 Ecologie Microbienne, IFR41 Bio-Environnement et Santé, Université Lyon 1, 43 bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France; St. Petersburg Nuclear Physics Institute RAS, St. Petersburg-Gatchina 188300, Russian Federation; LGGE CNRS, 54, rue Molière, Saint Martin d'Hères 38402, France; UMR6197 Lab. de Microbiologie des Environnements Extremes (LM2E) Universite de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Technopôle Brest Iroise, Place Nicolas Copernic, 29280 Plouzané, France; Centre de Génétique Moléculaire et Cellulaire/UMR-CNRS 5534, Université Lyon I, 43 bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France},\r\nabstract={The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular-phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene (rrs) targeted primers. As a result, rrs-targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus (< 1%). In addition, RubisCO (cbbL or rbcL) and NiFe-Hydrogenase (hoxV or hupL) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus. All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells.},\r\ncorrespondence_address1={Normand, P.; UMR-CNRS 5557 Ecologie Microbienne, IFR41 Bio-Environnement et Santé, Université Lyon 1, 43 bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France; email: normand@biomserv.univ-lyon1.fr},\r\npublisher={Blackwell Publishing Ltd},\r\nissn={14622912},\r\ncoden={ENMIF},\r\npubmed_id={17107552},\r\nlanguage={English},\r\nabbrev_source_title={Environ. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Exploring subglacial antarctic lake environments.\n \n \n \n \n\n\n \n Priscu, J.; Kennicutt II, M.; Bell, R.; Bulat, S.; Ellis-Evans, J.; Lukin, V.; Petit, J.; Powell, R.; Siegert, M.; and Tabacco, I.\n\n\n \n\n\n\n Eos, 86(20): 193+197. 2005.\n cited By 22\n\n\n\n
\n\n\n\n \n \n $\"Exploring$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Priscu2005,\r\nauthor={Priscu, J.C. and Kennicutt II, M.C. and Bell, R.E. and Bulat, S.A. and Ellis-Evans, J.C. and Lukin, V.V. and Petit, J.-R. and Powell, R.D. and Siegert, M.J. and Tabacco, I.},\r\ntitle={Exploring subglacial antarctic lake environments},\r\njournal={Eos},\r\nyear={2005},\r\nvolume={86},\r\nnumber={20},\r\npages={193+197},\r\ndoi={10.1029/2005EO200001},\r\nnote={cited By 22},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745909061&doi=10.1029%2f2005EO200001&partnerID=40&md5=79495022569536fb467ae428f73daebd},\r\naffiliation={Department of Land Resources and Environmental Science, Montana State University, Bozeman, United States; Office of the Vice President for Research, Texas A and M University, College Station, United States; Lamont-Doherty Earth Observatory, Palisades, NY, United States; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, St. Petersburg, Russian Federation; British Antarctic Survey, Cambridge, United Kingdom; Arctic and Antarctic Research Institute, St. Petersburg, Russian Federation; LGGE, Centre National de la Recherche Scientifique, Cedex, France; Northern Illinois University, DeKalb, United States; Bristol Glaciology Center, School of Geographical Sciences, University of Bristol, United Kingdom; DST-Geofica, Milan, Italy},\r\ncorrespondence_address1={Priscu, J.C.; Department of Land Resources and Environmental Science, Montana State University, Bozeman, MT, United States; email: jpriscu@montana.edu},\r\npublisher={Blackwell Publishing Ltd},\r\nissn={00963941},\r\nlanguage={English},\r\nabbrev_source_title={Eos},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2004\n \n \n (3)\n \n \n

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\n \n\n \n \n \n \n \n \n Phylogenetic relationship of Fusarium langsethiae to Fusarium poae and Fusarium sporotrichioides as inferred by IGS, ITS, β-tubulin sequences and UP-PCR hybridization analysis.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n International Journal of Food Microbiology, 95(3): 267-285. 2004.\n cited By 76\n\n\n\n
\n\n\n\n \n \n $\"Phylogenetic$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n Evolutionary relationships within 'pygmaeus' group microphallids using genetic analysis and scanning electron microscopy.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Journal of Helminthology, 78(3): 231-236. 2004.\n cited By 8\n\n\n\n
\n\n\n\n \n \n $\"Evolutionary$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n DNA signature of thermophilic bacteria from the aged accretion ice of Lake Vostok, Antarctica: Implications for searching for life in extreme icy environments.\n \n \n \n \n\n\n \n Bulat, S.; Alekhina, I.; Blot, M.; Petit, J.; de Angelis, M.; Wagenbach, D.; Lipenkov, V.; Vasilyeva, L.; Wloch, D.; Raynaud, D.; and Lukin, V.\n\n\n \n\n\n\n International Journal of Astrobiology, 3(1): 1-12. 2004.\n cited By 75\n\n\n\n
\n\n\n\n \n \n $\"DNA$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat20041,\r\nauthor={Bulat, S.A. and Alekhina, I.A. and Blot, M. and Petit, J.-R. and de Angelis, M. and Wagenbach, D. and Lipenkov, V.Y. and Vasilyeva, L.P. and Wloch, D.M. and Raynaud, D. and Lukin, V.V.},\r\ntitle={DNA signature of thermophilic bacteria from the aged accretion ice of Lake Vostok, Antarctica: Implications for searching for life in extreme icy environments},\r\njournal={International Journal of Astrobiology},\r\nyear={2004},\r\nvolume={3},\r\nnumber={1},\r\npages={1-12},\r\ndoi={10.1017/S1473550404001879},\r\nnote={cited By 75},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-25844439256&doi=10.1017%2fS1473550404001879&partnerID=40&md5=9f41e0a919e676ddad948a9cd069e1e4},\r\naffiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Leningrad region, Gatchina 188300, Russian Federation; Plasticité et Expression des Génomes Microbiens, UJF, CNRS, Grenoble 38041, France; Laboratoire de Glaciologie, Géophysique de l'Environnement, CNRS, BP96, Saint Martin d'Hères 38402, France; Institut für Umweltphysik, INF 229 Heidelberg 69120, Germany; Arctic and Antarctic Research Institute, 38 Bering Str., St. Petersburg 199397, Russian Federation},\r\nabstract={We have used 16S ribosomal genes to estimate the bacterial contents of Lake Vostok accretion ice samples at 3551 m and 3607 m, both containing sediment inclusions and formed 20000–15000 yr ago. Decontamination proved to be a critical issue, and we used stringent ice chemistry-based procedures and comprehensive biological controls in order to restrain contamination. As a result, up to now we have only recognized one 16S rDNA bacterial phylotype with confident relevance to the lake environment. It was found in one sample at 3607 m depth and represents the extant thermophilic facultative chemolithoautotroph Hydrogenophilus thermoluteolus of beta-Proteobacteria, and until now had only been found in hot springs. No confident findings were detected in the sample at 3551 m, and all other phylotypes revealed (a total of 16 phylotypes, 336 clones including controls) are presumed to be contaminants. It seems that the Lake Vostok accretion ice is actually microbe-free, indicating that the water body should also be hosting a highly sparse life. The message of thermophilic bacteria suggests that a geothermal system exists beneath the cold water body of Lake Vostok, what is supported by the geological setting, the long-term seismotectonic evidence from 4He degassing and the ‘18O shift’ of the Vostok accretion ice. The seismotectonic activity that seems to operate in deep faults beneath the lake could sustain thermophilic chemolithoautotrophic microbial communities. Such a primary production scenario for Lake Vostok may have relevance for icy planets and the approaches used for estimating microbial contents in accretion ice are clearly relevant for searching for extraterrestrial life. © 2004, Cambridge University Press. All rights reserved.},\r\nauthor_keywords={accretion ice;  Antarctica;  bacteria;  contamination;  exobiology;  geothermal environment;  Lake Vostok;  subglacial lake environments;  thermophiles},\r\nissn={14735504},\r\nlanguage={English},\r\nabbrev_source_title={Int. J. Astrobiology},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2003\n \n \n (3)\n \n \n

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\n \n\n \n \n \n \n \n \n Differentiation of six sibling species in the Saccharomyces sensu stricto complex by multilocus enzyme electrophoresis and UP-PCR analysis.\n \n \n \n \n\n\n \n Naumova, E.; Bulat, S.; Mironenko, N.; and Naumov, G.\n\n\n \n\n\n\n Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 83(2): 155-166. 2003.\n cited By 24\n\n\n\n
\n\n\n\n \n \n $\"Differentiation$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Naumova2003155,\r\nauthor={Naumova, E.S. and Bulat, S.A. and Mironenko, N.V. and Naumov, G.I.},\r\ntitle={Differentiation of six sibling species in the Saccharomyces sensu stricto complex by multilocus enzyme electrophoresis and UP-PCR analysis},\r\njournal={Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology},\r\nyear={2003},\r\nvolume={83},\r\nnumber={2},\r\npages={155-166},\r\ndoi={10.1023/A:1023328320228},\r\nnote={cited By 24},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037955955&doi=10.1023%2fA%3a1023328320228&partnerID=40&md5=cafaa86fb48d64263e2f739c1009ad31},\r\naffiliation={State Institute for Genetics, Selection of Indust. Microorganisms, Dorozhnyi 1, Moscow 113545, Russian Federation; Petersburg Nuclear Physics Institute, Gatchina 188350, Russian Federation; All-Russian Plant Protection Inst., St. Petersburg-Pushkin 189620, Russian Federation},\r\nabstract={UP-PCR analysis and multilocus enzyme electrophoresis were used to characterize 37 strains of the sibling species Saccharomyces cerevisiae, S. bayanus, S. cariocanus, S. kudriavzevii, S. mikatae and S. paradoxus. The results demonstrate that both molecular approaches are useful for discriminating between these phenotypically indistinguishable Saccharomyces species. The data obtained are in excellent agreement with previously reported genetic analyses, sequencing of the 18S rRNA and ITS regions, and DNA-DNA reassociation data.},\r\nauthor_keywords={Biological species;  MLEE;  Saccharomyces sensu stricto;  UP-PCR},\r\ncorrespondence_address1={Naumov, G.I.; State Institute for Genetics, Selection of Indust. Microorganism, Dorozhnyi 1, Moscow 113545, Russian Federation; email: gnaumov@yahoo.com},\r\nissn={00036072},\r\ncoden={ALJMA},\r\npubmed_id={12785309},\r\nlanguage={English},\r\nabbrev_source_title={Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n An International Plan for Antarctic Subglacial Lake Exploration.\n \n \n \n \n\n\n \n Priscu, J.; Bell, R.; Bulat, S.; Ellis-Evans, C.; Kennicutt, M.; Lukin, V.; Petit, J.; Powell, R.; Siegert, M.; and Tabacco, I.\n\n\n \n\n\n\n Polar Geography, 27(1): 69-83. 2003.\n cited By 31\n\n\n\n
\n\n\n\n \n \n $\"An$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Priscu200369,\r\nauthor={Priscu, J.C. and Bell, R.E. and Bulat, S.A. and Ellis-Evans, C.J. and Kennicutt, M.C. and Lukin, V.V. and Petit, J.-R. and Powell, R.D. and Siegert, M.J. and Tabacco, I.},\r\ntitle={An International Plan for Antarctic Subglacial Lake Exploration},\r\njournal={Polar Geography},\r\nyear={2003},\r\nvolume={27},\r\nnumber={1},\r\npages={69-83},\r\ndoi={10.1080/789610223},\r\nnote={cited By 31},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-2442421227&doi=10.1080%2f789610223&partnerID=40&md5=7005b8cf3dbd2e8b726356e3ea6b90ff},\r\naffiliation={Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, 59717, United States; Lamont-Doherty Earth Observatory, Palisades, NY, 10964, United States; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute (PNPI), RAS, Gatchina, 188350, Russian Federation; British Antarctic Survey, High Cross Site, Madingley Road, Cambridge, CB3 OET, United Kingdom; Geochemical and Environmental Research Group, Texas A&M University, College Station, TX, 77843, United States; Arctic and Antarctic Research Institute, 38 Bering Str, St. Petersburg, 199397, Russian Federation; LGGE-NCRS BP96, St. Martin D’Hères, F-38402, France; Department of Geology, Environmental Geosciences, Northern Illinois University, DeKalb, IL, 60115, United States; Bristol Glaciology Center, School of Geographical Sciences, University of Bristol, Bristol, BS8 1SS, United Kingdom; DST-Geofica, Via Cicoconara 7, Milano, 20129, Italy},\r\nabstract={Discovery of at least 100 subglacial lakes beneath the vast East Antarctic Ice Sheet has focused international attention on the challenges presented by the way we conduct science in such unique and inhospitable settings in an atmosphere of increasingly stringent environmental concerns. Exploration of subglacial environments will require careful and detailed planning, organization, and international cooperation. To this end, the Scientific Committee on Antarctic Research (SCAR) convened an international Group of Specialists (Subglacial Antarctic Lake Exploration Group of Specialists—SALEGOS) to develop a detailed assessment of the needs and critical milestones to be accomplished during the implementation of a subglacial exploration and research program. This paper surveys the progress and recommendations made by SALEGOS since its inception regarding the current state of knowledge of subglacial environments, technological needs and challenges, international management, the portfolio of scientific projects, and “clean” requirements for entry, observatory deployment, and sample retrieval. © 2003 Taylor & Francis Group, LLC.},\r\ncorrespondence_address1={Priscu, J.C.; Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, 59717, United States},\r\nissn={1088937X},\r\nlanguage={English},\r\nabbrev_source_title={Polar Geogr.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Trace elements in accreted ice from the Vostok sub-glacial lake, Antarctica: Initial results.\n \n \n \n \n\n\n \n Planchon, F.; Barbante, C.; Boutron, C.; Bulat, S.; Cescon, P.; Cozzi, G.; Dommergue, A.; Ferrari, C.; Gabrielli, P.; and Petit, J.\n\n\n \n\n\n\n 2003.\n cited By 0; Conference of XII International Conference on Heavy Metals in the Environment ; Conference Date: 26 May 2003 Through 30 May 2003; Conference Code:61099\n\n\n\n
\n\n\n\n \n \n $\"Trace$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@CONFERENCE{Planchon20031067,\r\nauthor={Planchon, F.A.M. and Barbante, C. and Boutron, C.F. and Bulat, S. and Cescon, P. and Cozzi, G. and Dommergue, A. and Ferrari, C.P. and Gabrielli, P. and Petit, J.R.},\r\ntitle={Trace elements in accreted ice from the Vostok sub-glacial lake, Antarctica: Initial results},\r\njournal={Journal De Physique. IV : JP},\r\nyear={2003},\r\nvolume={107},\r\nnumber={II},\r\npages={1067-1070},\r\ndoi={10.1051/jp4:20030484},\r\nnote={cited By 0; Conference of XII International Conference on Heavy Metals in the Environment ; Conference Date: 26 May 2003 Through 30 May 2003;  Conference Code:61099},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037700188&doi=10.1051%2fjp4%3a20030484&partnerID=40&md5=c681231f1ea679f92a1175b67109bd87},\r\naffiliation={LGGE of CNRS, Grenoble, France; Environmental Sciences Department, University Ca'Foscari of Venice, Venice, Italy; Petersburg Nuclear Physics Institute, Leningrad Region, Gatchina 188350, Russian Federation},\r\nabstract={We present in this study initial results on trace elements concentrations changes in the deepest part of the Vostok ice core. From 3310 m depth to the bottom of the ice core (3623 m), ice encountered is made of disturbed glacial ice by glacier dynamics and accreted ice from the sub-glacial lake Vostok. Since trace elements are involved in various biogeochemical processes, studying these elements in the accreted ice of the Vostok lake can lead to a better understanding of lake chemistry and life development in such extreme environment. Because concentrations to be measured in Antarctic ice are exceedingly low, such investigations on trace elements require strict control of contamination problems and the use of ultraclean protocols. Initial results obtained for Zn concentrations changes show high variability (< 0.5 to 32 pg/g), with the lowest concentrations found in the accreted ice of the Vostok lake (< 0.5 pg/g). According to crustal enrichment factors close to unity, Zn seems to be mainly associated with crustal material.},\r\ncorrespondence_address1={Planchon, F.A.M.; LGGE of CNRS, Grenoble, France},\r\nsponsors={},\r\npublisher={EDP Sciences},\r\naddress={Grenoble},\r\nissn={11554339},\r\ncoden={JPICE},\r\nlanguage={English},\r\nabbrev_source_title={J Phy IV JP},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 2002\n \n \n (2)\n \n \n

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\n \n\n \n \n \n \n \n \n Glass slide smears and imprints from infected organs for identification of Leishmania major by polymerase chain reaction methods [Vozmozhnost' ispol'zovaniia mazkov i otpechatkov na stekle ot porazhennykh organov dlia identifikatsii Leishmaniia major metodami PTSR analiza.].\n \n \n \n \n\n\n \n Strelkova, M.; Shendrik, A.; Bulat, S.; and Dergacheva, T.\n\n\n \n\n\n\n Meditsinskaia parazitologiia i parazitarnye bolezni, (4): 37-39. 2002.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Glass$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Strelkova200237,\r\nauthor={Strelkova, M.V. and Shendrik, A.G. and Bulat, S.A. and Dergacheva, T.I.},\r\ntitle={Glass slide smears and imprints from infected organs for identification of Leishmania major by polymerase chain reaction methods [Vozmozhnost' ispol'zovaniia mazkov i otpechatkov na stekle ot porazhennykh organov dlia identifikatsii Leishmaniia major metodami PTSR analiza.]},\r\njournal={Meditsinskaia parazitologiia i parazitarnye bolezni},\r\nyear={2002},\r\nnumber={4},\r\npages={37-39},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036825195&partnerID=40&md5=a2bc1b55d669c961be20285bb295f3b4},\r\nabstract={The paper presents data showing that the DNA isolated from the smears and imprints of L. major-infected hamsters is suitable for use in the polymerase chain reaction (PCR) to detect the causative agent of leishmaniasis. The most solid data have been obtained with the smears unexposed to staining and examination by using immersion oil and to benzene treatment. The DNA isolated from these smears infected may preserve for at least 1.5 months in a domestic refrigerator. The immersion oil-treated smears may be also used to identify leishmanias, but DNA should be isolated from the infected specimens of these smears just before PCR. The original primer pair L-unit/L-mail that has shown itself well in the experiments on cultured promastigotes may be, if required, used to differentiate L. major and L. turanica in the infected material collected from infected rodents.},\r\ncorrespondence_address1={Strelkova, M.V.},\r\nissn={00258326},\r\npubmed_id={12557586},\r\nlanguage={Russian},\r\nabbrev_source_title={Med Parazitol (Mosk)},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Molecular, morphological and phylogenetic analysis of the Fusarium avenaceum/F. arthrosporioides/F. Tricinctum species complex-A polyphasic approach.\n \n \n \n \n\n\n \n Yli-Mattila, T.; Paavanen-Huhtala, S.; Bulat, S.; Alekhina, I.; and Nirenberg, H.\n\n\n \n\n\n\n Mycological Research, 106(6): 655-669. 2002.\n cited By 70\n\n\n\n
\n\n\n\n \n \n $\"Molecular,$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Yli-Mattila2002655,\r\nauthor={Yli-Mattila, T. and Paavanen-Huhtala, S. and Bulat, S.A. and Alekhina, I.A. and Nirenberg, H.I.},\r\ntitle={Molecular, morphological and phylogenetic analysis of the Fusarium avenaceum/F. arthrosporioides/F. Tricinctum species complex-A polyphasic approach},\r\njournal={Mycological Research},\r\nyear={2002},\r\nvolume={106},\r\nnumber={6},\r\npages={655-669},\r\ndoi={10.1017/S0953756202006020},\r\nnote={cited By 70},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036617386&doi=10.1017%2fS0953756202006020&partnerID=40&md5=3fcf4edafa3b5f78e9b489015251bf1b},\r\naffiliation={Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, FIN-20014 Turku, Finland; Laboratory of Eukaryote Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Gatchina 188350, Russian Federation; Institut fuX r Mikrobiologie, BBA, Konigin-Luise-Strasse 19, D-14195, Berlin, Germany},\r\nabstract={Differences in morphology, ITS, IGS, mtSSU and β-tubulin sequences and UP-PCR hybridization were compared between morphologically identified F. avenaceum, F. arthrosporioides, F. anguioides, F. tricinctum, F. graminum and F. acuminatum strains. According to the combined β-tubulin, IGS and ITS tree, the strains of the Fusarium avenaceum/F. arthrosporioides/F. tricinctum species complex species can be divided into seven clusters supported by bootstrap values higher than 50%. The two main groups of European F. avenaceum, which cannot be distinguished by morphology, were separated in the tree based on β-tubulin sequences and less clearly in trees based on IGS and ITS sequences. MtSSU sequences were identical in all F. avenaceum and F. tricinctum strains studied. The European F. avenaceum strains of main group II had identical β-tubulin sequences with one American F. avenaceum strain and four European F. arthrosporioides strains, while F. avenaceum strains of main group I were closely related to two European F. arthrosporioides strains and to one Japanese F. anguioides strain. According to the combined β-tubulin/IGS/ITS sequence tree, European F. arthrosporioides strains were divided into four groups; F. tricinctum strains formed a well-supported cluster, in which two European clusters were separated from one African isolate. In the IGS sequence tree two European F. acuminatum strains together with one American F. acuminatum strain formed a cluster, which was separate from another American F. acuminatum strain. The F. acuminatum cluster was nested within the large F. tricinctum cluster together with one F. reticulatum strain in the combined IGS/β-tubulin tree. Several strains may be intermediates between the F. avenaceum/F. arthrosporioides/F. anguioides and F. tricinctum clusters and represent their own species. These results are partially supported by the results of UP-PCR hybridization analysis. Thus the molecular results may be helpful in future revision in the taxonomy of these species.},\r\nissn={09537562},\r\nlanguage={English},\r\nabbrev_source_title={Mycol. Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n Differences in morphology, ITS, IGS, mtSSU and β-tubulin sequences and UP-PCR hybridization were compared between morphologically identified F. avenaceum, F. arthrosporioides, F. anguioides, F. tricinctum, F. graminum and F. acuminatum strains. According to the combined β-tubulin, IGS and ITS tree, the strains of the Fusarium avenaceum/F. arthrosporioides/F. tricinctum species complex species can be divided into seven clusters supported by bootstrap values higher than 50%. The two main groups of European F. avenaceum, which cannot be distinguished by morphology, were separated in the tree based on β-tubulin sequences and less clearly in trees based on IGS and ITS sequences. MtSSU sequences were identical in all F. avenaceum and F. tricinctum strains studied. The European F. avenaceum strains of main group II had identical β-tubulin sequences with one American F. avenaceum strain and four European F. arthrosporioides strains, while F. avenaceum strains of main group I were closely related to two European F. arthrosporioides strains and to one Japanese F. anguioides strain. According to the combined β-tubulin/IGS/ITS sequence tree, European F. arthrosporioides strains were divided into four groups; F. tricinctum strains formed a well-supported cluster, in which two European clusters were separated from one African isolate. In the IGS sequence tree two European F. acuminatum strains together with one American F. acuminatum strain formed a cluster, which was separate from another American F. acuminatum strain. The F. acuminatum cluster was nested within the large F. tricinctum cluster together with one F. reticulatum strain in the combined IGS/β-tubulin tree. Several strains may be intermediates between the F. avenaceum/F. arthrosporioides/F. anguioides and F. tricinctum clusters and represent their own species. These results are partially supported by the results of UP-PCR hybridization analysis. Thus the molecular results may be helpful in future revision in the taxonomy of these species.\n

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\n \n\n \n \n \n \n \n \n Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay.\n \n \n \n \n\n\n \n Bulat, S.; Lubeck, M.; Alekhina, I.; Jensen, D.; Knudsen, I.; and Lubeck, P.\n\n\n \n\n\n\n Applied and Environmental Microbiology, 66(11): 4758-4763. 2000.\n cited By 52\n\n\n\n
\n\n\n\n \n \n $\"Identification$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat20004758,\r\nauthor={Bulat, S.A. and Lubeck, M. and Alekhina, I.A. and Jensen, D.F. and Knudsen, I.M.B. and Lubeck, P.S.},\r\ntitle={Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay},\r\njournal={Applied and Environmental Microbiology},\r\nyear={2000},\r\nvolume={66},\r\nnumber={11},\r\npages={4758-4763},\r\ndoi={10.1128/AEM.66.11.4758-4763.2000},\r\nnote={cited By 52},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033765725&doi=10.1128%2fAEM.66.11.4758-4763.2000&partnerID=40&md5=6922aa3468b1bf3e7e4e908e6a9bf76d},\r\naffiliation={Plant Pathology Section, Department of Plant Biology, Royal Veterinary/Agricultural Univ., Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark},\r\nabstract={We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was identified for strain GR5. No significant sequence homology was found between this marker and any other sequences in the databases. Southern blot analysis of the PCR product revealed that the marker represented a single-copy sequence specific for strain GR5. The marker was converted into a sequence-characterized amplified region (SCAR), and a specific PCR primer pair was designed. Eighty-two strains, isolated primarily from Danish soils, and 31 soil samples, originating from different localities, were tested, and this specificity was confirmed. Two strains responded to the SCAR primers under suboptimal PCR conditions, and the amplified sequences from these strains were similar, but not identical, to the GR5 marker. Soil assays in which total DNA was extracted from GR5-infested and noninoculated field soils showed that the SCAR primers could detect GR5 in a pool of mixed DNA and that no other soil microorganisms present contained sequences amplified by the primers. The assay developed will be useful for monitoring biological control agents released into natural field soil.},\r\ncorrespondence_address1={Lubeck, M.; Plant Pathology Section, Department of Plant Biology, Royal Veterinary/Agricultural Univ., Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark; email: met@kvl.dk},\r\nissn={00992240},\r\ncoden={AEMID},\r\npubmed_id={11055920},\r\nlanguage={English},\r\nabbrev_source_title={Appl. Environ. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Intraspecific variation in gamma-radiation resistance and genomic structure in the filamentous fungus Alternaria alternata: A case study of strains inhabiting chernobyl reactor no. 4.\n \n \n \n \n\n\n \n Mironenko, N.; Alekhina, I.; Zhdanova, N.; and Bulat, S.\n\n\n \n\n\n\n Ecotoxicology and Environmental Safety, 45(2): 177-187. 2000.\n cited By 51\n\n\n\n
\n\n\n\n \n \n $\"Intraspecific$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Mironenko2000177,\r\nauthor={Mironenko, N.V. and Alekhina, I.A. and Zhdanova, N.N. and Bulat, S.A.},\r\ntitle={Intraspecific variation in gamma-radiation resistance and genomic structure in the filamentous fungus Alternaria alternata: A case study of strains inhabiting chernobyl reactor no. 4},\r\njournal={Ecotoxicology and Environmental Safety},\r\nyear={2000},\r\nvolume={45},\r\nnumber={2},\r\npages={177-187},\r\ndoi={10.1006/eesa.1999.1848},\r\nnote={cited By 51},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033950167&doi=10.1006%2feesa.1999.1848&partnerID=40&md5=e787f28cee3a2e31bdc58869046a9fb5},\r\naffiliation={All-Russ. Plant Protection Institute, Podbelsky str. 3, 189620, Saint-Petersburg, Russian Federation; Dept. of Molec. and Radiat. Biophys., Petersburg Nucl. Phys. Inst. (PNPI), 188350, Gatchina, Russian Federation; Inst. Microbiol. Virol. Acad. Sci., 252143, Kiev, Ukraine},\r\nabstract={This is probably the first report on intraspecific variation in radiation resistance for filamentous fungi. It was revealed that natural ('field') strains of the filamentous fungus Alternaria alternata are extremely variable in response to gamma-irradiation ranging from supersensitive to highly resistant to radiation. At the same time nearly all strains originating from the highly radiation-polluted reactor of the Chernobyl (Ukraine) Nuclear Power Plant possessed high radiation resistance. The genome structure of strains studied by universally primed polymerase chain reaction (UP-PCR) was found to be well conserved in 'reactor' but not in 'control' strains. The 'reactor' strains appear to be genetically adapted to this high radiation habitat by means of selection, thus providing a natural source of genetically homogeneous fungal lineages.},\r\nauthor_keywords={Adaptation;  Alternaria alternata;  Chernobyl;  Fungi;  Gamma-radiation resistance;  Genomic diversity;  UP-PCR},\r\ncorrespondence_address1={Bulat, S.A.; Dept. of Molec./Radiation Biophysics, PNPI, 188350 Gatchina, Russian Federation; email: bulat@dmrb.pnpi.spb.ru},\r\nissn={01476513},\r\ncoden={EESAD},\r\npubmed_id={10648134},\r\nlanguage={English},\r\nabbrev_source_title={Ecotoxicol. Environ. Saf.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n\n\n\n\n\n

\n

\n \n 1999\n \n \n (2)\n \n \n

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\n \n \n

\n \n\n \n \n \n \n \n \n Classification of trypanosomatids from insects and plants by the UP-PCR (universally primed PCR) technique and cross dot blot hybridization of PCR products.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n European Journal of Protistology, 35(3): 319-326. 1999.\n cited By 19\n\n\n\n
\n\n\n\n \n \n $\"Classification$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n\n \n \n \n \n \n \n RAPD-PCR, isozyme, rDNA RFLP and rDNA sequence analyses in identification of Finnish Fusarium oxysporum isolates.\n \n \n \n \n\n\n \n Paavanen-Huhtala, S.; Hyvönen, J.; Bulat, S.; and Yli-Mattila, T.\n\n\n \n\n\n\n Mycological Research, 103(5): 625-634. 1999.\n cited By 37\n\n\n\n
\n\n\n\n \n \n $\"RAPD-PCR,$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Paavanen-Huhtala1999625,\r\nauthor={Paavanen-Huhtala, S. and Hyvönen, J. and Bulat, S.A. and Yli-Mattila, T.},\r\ntitle={RAPD-PCR, isozyme, rDNA RFLP and rDNA sequence analyses in identification of Finnish Fusarium oxysporum isolates},\r\njournal={Mycological Research},\r\nyear={1999},\r\nvolume={103},\r\nnumber={5},\r\npages={625-634},\r\ndoi={10.1017/S0953756298007485},\r\nnote={cited By 37},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0342635466&doi=10.1017%2fS0953756298007485&partnerID=40&md5=a83eaa49e8439a0afdc54c6d4b2d288b},\r\naffiliation={Laboratory of Eukaryote Genetics, Dept. of Molec. and Radiat. Biophys., Petersburg Nuclear Physics Institute, Gatchina 183350, Russian Federation; Lab. Plant Physiol. and Molec. Biol., Department of Biology, FIN-20014 Turku, Finland; Department of Biology, FIN-20014 Turku, Finland},\r\nabstract={Twenty seven Fusarium oxysporum isolates were studied by RAPD-PCR and isozyme analyses. Thirteen isolates were from barley and the rest from different hosts, most of which were dicotyledonous plants. All isolates could be distinguished from each other by RAPD-PCR analysis, and clustered into seven groups in NJ and parsimony consensus trees. Isozyme analysis detected polymorphism in five of the six enzymes and the isolates could be divided into 26 different electrophoretic groups. Five groups were supported by high branch support and bootstrap values in the approximate support tree of combined RAPD-PCR and isozyme data. These five groups were found also in NJ and parsimony consensus trees. The matrices from RAPD-PCR and isozyme data proved to be incongruent, but they did not totally disprove each other. Some correlation was found between geographical origin and phylogenetic relationships of isolates collected from barley. Representatives of the main clades of phylogenetic trees, were further studied by rDNA RFLP and rDNA sequence analyses, together with isolates of other Fusarium species. Isolates of F. oxysporum and F. avenaceum formed distinct groups in the phylogenetic analyses, except for two isolates of F. oxysporum which were grouped with isolates of F. redolens.},\r\n}

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\n\n\n\n\n\n

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\n \n 1998\n \n \n (2)\n \n \n

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\n \n \n

\n \n\n \n \n \n \n \n \n Towards molecular systematics of trypanosomatids from insects by means of the polymerase chain reaction with universal primers (UP-PCR).\n \n \n \n \n\n\n \n Podlipaev, S.; Mokrousov, I.; and Bulat, S.\n\n\n \n\n\n\n Parazitologiya, 32(4): 324-326. 1998.\n cited By 1\n\n\n\n
\n\n\n\n \n \n $\"Towards$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Podlipaev1998324,\r\nauthor={Podlipaev, S.A. and Mokrousov, I.V. and Bulat, S.A.},\r\ntitle={Towards molecular systematics of trypanosomatids from insects by means of the polymerase chain reaction with universal primers (UP-PCR)},\r\njournal={Parazitologiya},\r\nyear={1998},\r\nvolume={32},\r\nnumber={4},\r\npages={324-326},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042847488&partnerID=40&md5=10aecba0b05ff067623c4befb3007a2c},\r\nabstract={The results of the first using of universal primer - polimerase chain reaction (UP-PSR) for the classification of trypanosomatids from insects and plants are presented. The dendrograms of 14 trypanosomatids cultures from insects and 3 cultures from plants derived from genetic distances were obtained. Wide specificity of insect trypanosomatids is demonstrated - for example two isolates of parasites from different insects orders appeared to be more similiar than isolates from the same host insect species. The reality of genus Proteomonas is confirmed. The results demonstrate the high level of heterogeneity of the isolates referring to the genus Leptomonas which seems to be an artificial group. It is obvious that the amount of existing genera is not enough for the description of trypanosomatids biodiversity. The applied method allows to obtain the hierarchical clusters of isolates. Thus, the system of insects trypanosomatids may be discussed in terms of traditional taxonomy.},\r\nauthor_keywords={Polymerase chain reaction;  Systematics;  Trypanosomatids from insects;  Universal primers},\r\nissn={00311847},\r\ncoden={PAZGA},\r\nlanguage={Russian},\r\nabbrev_source_title={Parazitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

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\n \n\n \n \n \n \n \n \n UP-PCR analysis and ITS1 ribotyping of strains of Trichoderma and Gliocladium.\n \n \n \n \n\n\n \n \n\n\n \n\n\n\n Mycological Research, 102(8): 933-943. 1998.\n cited By 98\n\n\n\n
\n\n\n\n \n \n $\"UP-PCR$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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\n \n 1997\n \n \n (6)\n \n \n

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\n \n\n \n \n \n \n \n \n PCR diagnostics for pathogens of potato: Problems and perspectives.\n \n \n \n \n\n\n \n Mironenko, N.; and Bulat, S.\n\n\n \n\n\n\n Mikologiya I Fitopatologiya, 31(4): 72-85. 1997.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"PCR$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Mironenko199772,\r\nauthor={Mironenko, N.V. and Bulat, S.A.},\r\ntitle={PCR diagnostics for pathogens of potato: Problems and perspectives},\r\njournal={Mikologiya I Fitopatologiya},\r\nyear={1997},\r\nvolume={31},\r\nnumber={4},\r\npages={72-85},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0040059922&partnerID=40&md5=0a210dae83f9d92113f050e3381e2888},\r\nabstract={Problems and perspectives in PCR diagnosis of fungal potato pathogens are reviewed. The molecular genetic studies of these pathogens and related species as well as PCR assays already developed are discussed and critically commented. The examination performed has resulted in the statement that the diagnostics scheme based on (coupled with) PCR is the most applicable and of a great advance now and certainly further.},\r\nissn={00263648},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikol. Fitopatol.},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Universally primed polymerase chain reaction analysis of Fusarium avenaceum isolated from wheat and barley in Finland.\n \n \n \n \n\n\n \n Yli-Mattila, T.; Mironenko, N.; Alekhina, I.; Hannukkala, A.; and Bulat, S.\n\n\n \n\n\n\n Agricultural and Food Science in Finland, 6(1): 25-36. 1997.\n cited By 25\n\n\n\n
\n\n\n\n \n \n $\"Universally$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Yli-Mattila199725,\r\nauthor={Yli-Mattila, T. and Mironenko, N.V. and Alekhina, I.A. and Hannukkala, A. and Bulat, S.A.},\r\ntitle={Universally primed polymerase chain reaction analysis of Fusarium avenaceum isolated from wheat and barley in Finland},\r\njournal={Agricultural and Food Science in Finland},\r\nyear={1997},\r\nvolume={6},\r\nnumber={1},\r\npages={25-36},\r\nnote={cited By 25},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031490421&partnerID=40&md5=0dcc69bba321826b401e87e550198565},\r\naffiliation={Lab. Plant Physiol. Plant Molec. B., Department of Biology, FIN-20014 Turku, Finland; All-Russ. Plant Protection Institute, Lab. of Plant Immunity to the Pests, St. Petersburg 189620, Russian Federation; Komarov Botanical Institute, Laboratory of Fungal Biochemistry, St. Petersburg 197376, Russian Federation; Institute of Plant Protection, Agric. Research Centre of Finland, FIN-31600 Jokioinen, Finland; Petersburg Nucl. Phys. Inst. (PNPI), Dept. of Molec. and Radiat. Biophys., Gatchina 188350, Russian Federation},\r\nabstract={Twenty-two Fusarium avenaceuin isolates from Finnish wheat and barley were analysed using the chain reaction with universal primers (UP-PCR). Each isolate could be distinguished from others by UP-PCR products on polyacrylamide gels. The isolates tested were clustered into two main groups and further into several subgroups by UP-PCR profiles and phylogenetic analyses. The phylogenetic relationships of these groups are discussed. No clear correlation was found between the groups and host plant preference or the geographic origin of F. avenaceum isolates. Pathogenicity tests showed differences between F. avenaceum isolates, but two isolates, one from wheat and the other from barley, were the most aggressive in wheat and barley. This fungus, usually known as a weak pathogen of cereals and other crops, has thus probably not evolved in respect to its ability to damage wheat or barley.},\r\nauthor_keywords={Genotyping, parsimony analysis;  Gibberella avenacea;  Identification;  UP-PCR},\r\ncorrespondence_address1={Yli-Mattila, T.; Lab. Plant Physiol. Plant Molec. B., Department of Biology, FIN-20014 Turku, Finland; email: tymat@utu.fi},\r\nissn={12390992},\r\nlanguage={English},\r\nabbrev_source_title={Agric. Food Sci. Finl.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Molecular taxonomy of Fusarium fungi by means of ribotyping, rDNA sequencing and UP-PCR analysis. A case study of taxa in Sporotrichiella section.\n \n \n \n \n\n\n \n Bulat, S.; Alekhina, I.; Kozlova, E.; and Yli-Mattila, T.\n\n\n \n\n\n\n Cereal Research Communications, 25(3 II): 565-570. 1997.\n cited By 4\n\n\n\n
\n\n\n\n \n \n $\"Molecular$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat1997565,\r\nauthor={Bulat, S.A. and Alekhina, I.A. and Kozlova, E.D. and Yli-Mattila, T.},\r\ntitle={Molecular taxonomy of Fusarium fungi by means of ribotyping, rDNA sequencing and UP-PCR analysis. A case study of taxa in Sporotrichiella section},\r\njournal={Cereal Research Communications},\r\nyear={1997},\r\nvolume={25},\r\nnumber={3 II},\r\npages={565-570},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030663761&partnerID=40&md5=42dc03282915d6fc9ca2d7304aa40777},\r\naffiliation={Eukaryotes' Genetics Laboratory, Petersburg Nuclear Physics Institute, Gatchina, 188350, Russian Federation; Komarov Botanical Institute, Laboratory of Fungal Biochemistry, St.-Petersburg, 197376, Russian Federation; Lab. Plant Physiol. and Mol. Biol., University of Turku, FIN-20014 Turku, Finland},\r\ncorrespondence_address1={Bulat, S.A.; Eukaryotes' Genetics Laboratory, Petersburg Nuclear Physics Inst., Gatchina 188350, Russian Federation},\r\nissn={01333720},\r\ncoden={CRCMC},\r\nlanguage={English},\r\nabbrev_source_title={CEREAL RES. COMMUN.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n\n\n\n

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\n \n\n \n \n \n \n \n \n Comparison of Some Molecular-genetic Techniques for Identification of Leishmania Circulating in Natural Foci of Zoonotic Cutaneous Leishmaniasis in the Central Asia Region.\n \n \n \n \n\n\n \n Strelkova, M.; Pacheco, R.; Bulat, S.; and Rakitskaya, T.\n\n\n \n\n\n\n Memorias do Instituto Oswaldo Cruz, 92(1): 109-114. 1997.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"Comparison$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Strelkova1997109,\r\nauthor={Strelkova, M.V. and Pacheco, R.S. and Bulat, S.A. and Rakitskaya, T.A.},\r\ntitle={Comparison of Some Molecular-genetic Techniques for Identification of Leishmania Circulating in Natural Foci of Zoonotic Cutaneous Leishmaniasis in the Central Asia Region},\r\njournal={Memorias do Instituto Oswaldo Cruz},\r\nyear={1997},\r\nvolume={92},\r\nnumber={1},\r\npages={109-114},\r\ndoi={10.1590/S0074-02761997000100023},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030621950&doi=10.1590%2fS0074-02761997000100023&partnerID=40&md5=008fac106a0a83a2b52db4d871228cb9},\r\naffiliation={Depto. de Bioquim. e Biol. Molecular, Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil; Institute of Nuclear Physics, St. Petersburg, Russian Federation; Vavilov Inst. of General Genetics, Moscow, Russian Federation; Martsinovsky Inst. Med. P., Moscow, Russian Federation},\r\nabstract={Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization techniques are discussed in terms of their applicability, discrimination power and feasibility for answering questions related to molecular epidemiological research and for detecting mixed Leishmania infections.},\r\nauthor_keywords={Hybridization;  Isoenzymes;  kDNA;  Old World leishmaniasis;  PCR},\r\ncorrespondence_address1={Pacheco, R.S.; Depto. de Bioquim. e Biol. Molecular, Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil},\r\npublisher={Fundacao Oswaldo Cruz},\r\nissn={00740276},\r\npubmed_id={9302420},\r\nlanguage={English},\r\nabbrev_source_title={Mem. Inst. Oswaldo Cruz},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n RAPD-PCR, UP-PCR rDNA RFLP, rDNA sequence and isozyme analyses of Fusarium isolates in Finland.\n \n \n \n \n\n\n \n Yli-Maitila, T.; Paavanen-Huhtala, S.; Hannukkala, A.; Alekhina, I.; Hyvönen, J.; and Bulat, S.\n\n\n \n\n\n\n Cereal Research Communications, 25(3 II): 629-630. 1997.\n cited By 4\n\n\n\n
\n\n\n\n \n \n $\"RAPD-PCR,$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Yli-Maitila1997629,\r\nauthor={Yli-Maitila, T. and Paavanen-Huhtala, S. and Hannukkala, A. and Alekhina, I.A. and Hyvönen, J. and Bulat, S.A.},\r\ntitle={RAPD-PCR, UP-PCR rDNA RFLP, rDNA sequence and isozyme analyses of Fusarium isolates in Finland},\r\njournal={Cereal Research Communications},\r\nyear={1997},\r\nvolume={25},\r\nnumber={3 II},\r\npages={629-630},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030691819&partnerID=40&md5=bfa3e0bc92312e5dc9ab1c9519798bc7},\r\naffiliation={Dept. of Biology, Univ. of Turku, FIN-20014 Turku, Finland; Institute of Plant Protection, Agric. Research Centre of Finland, FIN-31600 Jokioinen, Finland; Komarov Botanical Institute, Laboratory of Fungal Biochemistry, St.-Petersburg 197376, Russian Federation; PNPI, Dept. of Molec. and Radiat. Biophys., Gatchina 1883 50, Russian Federation},\r\nabstract={Isozyme analysis and both PCR methods with random (RAPD-PCR) and random-like (UP-PCR) primers are suitable for intraspecific identification and phylogenetic studies in F. avenaceum and F. oxysporum. RAPD-PCR and UP-PCR can also be used for identification of Fusarium species. rDNA RFLP and rDNA sequence analyses were more suitable for identification and phylogenetic studies at species level, but their resolution at strain level was clearly poorer than in RAPD-PCR and UP-PCR analyses.},\r\ncorrespondence_address1={Yli-Mattila, T.; Dept of Biology, Univ. of Turku, FIN-20014 Turku, Finland},\r\nissn={01333720},\r\ncoden={CRCMC},\r\nlanguage={English},\r\nabbrev_source_title={CEREAL RES. COMMUN.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Genetic and molecular delineation of three sibling species in the Hansenula polymorpha complex.\n \n \n \n \n\n\n \n Naumov, G.; Naumova, E.; Kondratieva, V.; Bulat, S.; Mironenko, N.; Mendonça-Hagler, L.; and Hagler, A.\n\n\n \n\n\n\n Systematic and Applied Microbiology, 20(1): 50-56. 1997.\n cited By 46\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Naumov199750,\r\nauthor={Naumov, G.I. and Naumova, E.S. and Kondratieva, V.I. and Bulat, S.A. and Mironenko, N.V. and Mendonça-Hagler, L.C. and Hagler, A.N.},\r\ntitle={Genetic and molecular delineation of three sibling species in the Hansenula polymorpha complex},\r\njournal={Systematic and Applied Microbiology},\r\nyear={1997},\r\nvolume={20},\r\nnumber={1},\r\npages={50-56},\r\ndoi={10.1016/S0723-2020(97)80047-1},\r\nnote={cited By 46},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031052172&doi=10.1016%2fS0723-2020%2897%2980047-1&partnerID=40&md5=336fda0c6c62a7c3495e8a34a63f83f0},\r\naffiliation={State Inst. Genet. Sel. Indust. M., Moscow, Russian Federation; All-Russ. Plant Protection Institute, St-Petersburg-Pushkin, Russian Federation; Instituto de Microbiologia, Unversidad Federal do Rio de Janeiro, Rio de Janeiro, Brazil; State Inst. Genetic Sel. Indust. M., I Dorozhnyi 1, Moscow 113545, Russian Federation},\r\nabstract={Genetic hybridization, molecular karyotyping and UP-PCR analysis showed that the taxonomic complex Hansenula polymorpha De Morais et Maia consists of three biological sibling species. H. angusta Teunisson et al. is not synonymous with H. polymorpha and must be reinstated as a separate species. The third sibling species is apparently a new taxon associated with Opuntia cacti. The sibling species are able to cross with each other but their interspecific hybrids are sterile.},\r\nauthor_keywords={Genetic analysis;  Hansenula polymorpha complex;  Molecular karyotyping;  UP-PCR},\r\ncorrespondence_address1={Numov, G.I.; State Institute for Genetic, Selection Industrial Microorganisms, I Dorozhnyi 1, Moscow 113545, Russian Federation; email: gennadi@vnigen.msk.su},\r\npublisher={Elsevier GmbH},\r\nissn={07232020},\r\ncoden={SAMID},\r\nlanguage={English},\r\nabbrev_source_title={SYST. APPL. MICROBIOL.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n

\n \n 1996\n \n \n (2)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n Identification of Fungi and Analysis of Their Genetic Diversity by Polymerase Chain Reaction (PCR) with Gene-Specific and Nonspecific Primers.\n \n \n \n \n\n\n \n Bulat, S.; and Mironenko, N.\n\n\n \n\n\n\n Russian Journal of Genetics, 32(2): 143-159. 1996.\n cited By 15\n\n\n\n
\n\n\n\n \n \n $\"Identification$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat1996143,\r\nauthor={Bulat, S.A. and Mironenko, N.V.},\r\ntitle={Identification of Fungi and Analysis of Their Genetic Diversity by Polymerase Chain Reaction (PCR) with Gene-Specific and Nonspecific Primers},\r\njournal={Russian Journal of Genetics},\r\nyear={1996},\r\nvolume={32},\r\nnumber={2},\r\npages={143-159},\r\nnote={cited By 15},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0001588917&partnerID=40&md5=f9ac5e56c53f2d99e90e8633271057b4},\r\naffiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Leningradskayaoblast 188350, Russian Federation; All-Russia Res. Inst. Plant Protect., Pushkin, St. Petersburg 188620, Russian Federation},\r\nabstract={Modern molecular genetic methods for identification of fungi and analysis of their diversity, based on polymerase chain reaction (PCR) with gene-specific and nonspecific (random) primers, are reviewed. Applications of these PCR techniques are discussed on the basis of published data and our experimental results. During our discussion of PCR with nonspecific primers, main attention is paid to PCR with universal primers (UP-PCR) and the PCR-based technique of random amplification of polymorphic DNA (RAPD-PCR). Applications of PCR techniques are demonstrated for phytopathogenic fungi.},\r\ncorrespondence_address1={Bulat, S.A.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Leningradskayaoblast 188350, Russian Federation},\r\nissn={10227954},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Gen.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Identification of fungi and analysis of their genetic diversity by polymerase chain reaction (pcr) with gene-specific and nonspecific primers.\n \n \n \n \n\n\n \n Bulat, S.; and Mironenko, N.\n\n\n \n\n\n\n Genetika, 32(2): 165-183. 1996.\n cited By 11\n\n\n\n
\n\n\n\n \n \n $\"Identification$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat1996165,\r\nauthor={Bulat, S.A. and Mironenko, N.V.},\r\ntitle={Identification of fungi and analysis of their genetic diversity by polymerase chain reaction (pcr) with gene-specific and nonspecific primers},\r\njournal={Genetika},\r\nyear={1996},\r\nvolume={32},\r\nnumber={2},\r\npages={165-183},\r\nnote={cited By 11},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0000037139&partnerID=40&md5=c8d068bf2ba7696fb31a9336cdaadbbe},\r\naffiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Leningradskaya ablast, 188350, Russian Federation; All-Russian Research Institute of Plant Protection, Pushkin, St. Petersburg, 188620, Russian Federation},\r\nabstract={Modern molecular genetic methods for identification of fungi and analysis of their diversity, based on polymerase chain reaction (PCR) with gene-specific and nonspecific (random) primers, are reviewed. Applications of these PCR techniques are discussed on the basis of published data and our experimental results. During our discussion of PCR with nonspecific primers, main attention is paid to PCR with universal primers (UP-PCR) and the PCR-based technique of random amplification of polymorphic DNA (RAPD-PCR). Applications of PCR techniques are demonstrated for phytopathogenic fungi.},\r\ncorrespondence_address1={Bulat, S.A.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Leningradskaya ablast, 188350, Russian Federation},\r\nissn={00166758},\r\ncoden={GNKAA},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n

\n \n 1995\n \n \n (1)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n Genetic structure of soil population of fungus Fusarium oxysporum Schlechtend.: Fr.: Molecular reidentification of the species and genetic differentiation of isolates using polymerase chain reaction technique with universal primers (UP-PCR).\n \n \n \n \n\n\n \n Bulat, S.; Mironenko, N.; and Zholkevich Yu., G.\n\n\n \n\n\n\n Genetika, 31(3): 315-323. 1995.\n cited By 14\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat1995315,\r\nauthor={Bulat, S.A. and Mironenko, N.V. and Zholkevich Yu., G.},\r\ntitle={Genetic structure of soil population of fungus Fusarium oxysporum Schlechtend.: Fr.: Molecular reidentification of the species and genetic differentiation of isolates using polymerase chain reaction technique with universal primers (UP-PCR)},\r\njournal={Genetika},\r\nyear={1995},\r\nvolume={31},\r\nnumber={3},\r\npages={315-323},\r\nnote={cited By 14},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028988903&partnerID=40&md5=8681e21a43b95b8d69651f264059271f},\r\naffiliation={St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina 188350, Russian Federation},\r\nabstract={The genetic structure of three soil populations of fungus Fusarium oxysporum was analyzed using polymerase chain reaction with universal primers (UP-PCR). Distinct UP-PCR variants revealed by means of cross-dot hybridization of amplified DNA and restriction analysis of nuclear ribosomal DNA represent subspecies or sibling species of F. oxysporum. The remaining isolates of F. oxysporum showed moderate UP-PCR polymorphism characterized by numerous types, whose relatedness was analyzed by computer treatment of the UP-PCR patterns. The genetic distance trees based on the UP-PCR patterns, which were obtained with different universal primers, demonstrated similar topology. This suggests that evolutionarily important genome rearrangements correlatively occur within the entire genome. Isolates representing different UP-PCR polymorphisms were encountered in all populations, being distributed asymmetrically in two of these. In general, soil populations of F. oxysporum were represented by numerous genetically isolated groups with a similar genome structure. The genetic heterogeneity of the isolates within these groups is likely to be caused by the parasexual process. The usefulness of the UP-PCR technique for population studies of F. oxysporum was demonstrated.},\r\ncorrespondence_address1={Bulat, S.A.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina 188350, Russian Federation},\r\nissn={00166758},\r\ncoden={GNKAA},\r\npubmed_id={7607421},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n

\n \n 1993\n \n \n (3)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.].\n \n \n \n \n\n\n \n Bulat, S.; Filatov, M.; and Pantina, R.\n\n\n \n\n\n\n Tsitologiya, 35(6-7): 74-78. 1993.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat199374,\r\nauthor={Bulat, S.A. and Filatov, M.V. and Pantina, R.A.},\r\ntitle={The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.]},\r\njournal={Tsitologiya},\r\nyear={1993},\r\nvolume={35},\r\nnumber={6-7},\r\npages={74-78},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&partnerID=40&md5=233809336d07245daae6c4fc6a081fa1},\r\nabstract={The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids "human x Chinese hamster" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00413771},\r\npubmed_id={8266567},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n The production of clones of \"man x Chinese hamster\" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok \"chelovek x kitaǐskiǐ khomiachok\", soderzhashchikh raznye chasti genoma cheloveka.].\n \n \n \n \n\n\n \n Filatov, M.; Bulat, S.; Drobchenko, E.; Kotlovanova, L.; Pantina, R.; Semenova, E.; Stepanov, S.; Tret'iakov, A.; and Shcherbakova, O.\n\n\n \n\n\n\n Tsitologiya, 35(6-7): 68-73. 1993.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Filatov199368,\r\nauthor={Filatov, M.V. and Bulat, S.A. and Drobchenko, E.A. and Kotlovanova, L.V. and Pantina, R.A. and Semenova, E.V. and Stepanov, S.I. and Tret'iakov, A.N. and Shcherbakova, O.G.},\r\ntitle={The production of clones of "man x Chinese hamster" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok "chelovek x kitaǐskiǐ khomiachok", soderzhashchikh raznye chasti genoma cheloveka.]},\r\njournal={Tsitologiya},\r\nyear={1993},\r\nvolume={35},\r\nnumber={6-7},\r\npages={68-73},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&partnerID=40&md5=07712067d4e466fb44c89e58dfa85128},\r\nabstract={Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={8266566},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

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\n\n\n

\n \n\n \n \n \n \n \n \n Genetic differentiation of phytopathogenic fungus Cochliobolussativus (Ito and Kurib.) Drechsl. ex Dastur (Bipolaris sorokiniana (Sacc.:Sorok.) Shoem.) revealed by a Universally Primed Polymerase Chain Reaction UP-PCR technique: Correlation with host-specificity.\n \n \n \n \n\n\n \n Bulat, S.; and Mironenko, N.\n\n\n \n\n\n\n Genetika, 29(8): 1295-1301. 1993.\n cited By 2\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19931295,\r\nauthor={Bulat, S.A. and Mironenko, N.V.},\r\ntitle={Genetic differentiation of phytopathogenic fungus Cochliobolussativus (Ito and Kurib.) Drechsl. ex Dastur (Bipolaris sorokiniana (Sacc.:Sorok.) Shoem.) revealed by a Universally Primed Polymerase Chain Reaction UP-PCR technique: Correlation with host-specificity},\r\njournal={Genetika},\r\nyear={1993},\r\nvolume={29},\r\nnumber={8},\r\npages={1295-1301},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027440536&partnerID=40&md5=524e3c61da0c56f450ac0d1577c21595},\r\naffiliation={St. Petersb. Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation},\r\nabstract={Populations of C. sativus fungus isolated from barley (2 populations, Russia, Kazakhstan) and wheat (3 populations, Kazakhstan, Viet-Nam) have been analysed by UP-PCR technique. The oligoCs2 system consisted of pair universal primers of own construction was used. UP-PCR patterns of isolates of all populations have been divided into 8 UP-PCR polymorphisms. In 'barley' populations 6 polymorphisms were observed. One from them was represented by a great number of isolates. In 'wheat' populations there were 4 polymorphisms: the one, represented a majority of isolates, was found in barley also, but in a few cases, and 2 were unique and wheat-specific. The genetic relationship of isolates represented different PCR polymorphisms was appreciated by the KITSCH (UPGMA-like) and FITCH procedures of PHYLIP package. On the whole the correlation of UP-PCR polymorphisms with the host specificity of fungus is revealed.},\r\ncorrespondence_address1={Bulat, S.A.; St. Petersb. Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation},\r\nissn={00166758},\r\ncoden={GNKAA},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 1992\n \n \n (5)\n \n \n

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\n \n\n \n \n \n \n \n \n Comparative analysis of spontaneous mitotic recombination in [cir0] and [cir+] strains of the yeast Saccharomyces cerevisiae.\n \n \n \n \n\n\n \n Pushnova, E.; Bulat, S.; and Korolev, V.\n\n\n \n\n\n\n Current Genetics, 22(4): 259-265. 1992.\n cited By 2\n\n\n\n
\n\n\n\n \n \n $\"Comparative$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Pushnova1992259,\r\nauthor={Pushnova, E.A. and Bulat, S.A. and Korolev, V.G.},\r\ntitle={Comparative analysis of spontaneous mitotic recombination in [cir0] and [cir+] strains of the yeast Saccharomyces cerevisiae},\r\njournal={Current Genetics},\r\nyear={1992},\r\nvolume={22},\r\nnumber={4},\r\npages={259-265},\r\ndoi={10.1007/BF00317918},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026640603&doi=10.1007%2fBF00317918&partnerID=40&md5=03c6fca7a529a7ddfc68b545e8e01a2f},\r\naffiliation={Laboratory of Genetics of Eukaryot, Department of Molecular and Radiation Biophysics, St. Petersburg Nuclear Physics Institute, Academy of Sciences of Russia, Gatchina, Russian Federation},\r\nabstract={The influence of the 2 μm plasmid on homologous recombination in the right arm of chromosome XV of the yeast Saccharomyces cerevisiae has been examined. No differences between spontaneous mitotic recombination rates in [cir0] and [cir+] derivatives of two yeast diploid tester strains were detected. In the course of analysis an unusually high coincident conversion frequency at ADE2, HIS3, and two RFLP loci adjacent to ADE2, was observed. The character of coincident homozygotization of linked markers argues for a "break-and-replicate" mechanism underlying the coincident conversion events. © 1992 Springer-Verlag.},\r\nauthor_keywords={2 μm plasmid;  Coincident conversion;  Mitotic recombination;  Yeast},\r\ncorrespondence_address1={Pushnova, E.A.; Laboratory of Genetics of Eukaryot, Department of Molecular and Radiation Biophysics, St. Petersburg Nuclear Physics Institute, Academy of Sciences of Russia, Gatchina, Russian Federation},\r\npublisher={Springer-Verlag},\r\nissn={01728083},\r\ncoden={CUGED},\r\npubmed_id={1356638},\r\nlanguage={English},\r\nabbrev_source_title={Curr Genet},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n Biological species distinction of Aureobasidium pullulans (De Bary) fungus by universally primed polymerase chain reaction.\n \n \n \n \n\n\n \n Mokrousov, I.; and Bulat, S.\n\n\n \n\n\n\n Genetika, 28(4): 31-38. 1992.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"Biological$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Mokrousov199231,\r\nauthor={Mokrousov, I.V. and Bulat, S.A.},\r\ntitle={Biological species distinction of Aureobasidium pullulans (De Bary) fungus by universally primed polymerase chain reaction},\r\njournal={Genetika},\r\nyear={1992},\r\nvolume={28},\r\nnumber={4},\r\npages={31-38},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026756071&partnerID=40&md5=16756663d64e0d221fbcd94bbebb1901},\r\naffiliation={Chemical Pharmaceutical Institute, St. Petersburg, Russia},\r\nabstract={Genome structure of 46 collection isolates of Aureobasidium pullulans fungus and 2 isolates of A. microstictum was analysed by DNA hybridization variant of universally primed polymerase chain reaction (UP-PCR) technique, aimed at the study of their genetical homogeneity (to elucidate their general unity). As a result, A. pullulans isolates were divided into four original groups with DNA unhybridizable UP-PCR patterns. These groups are regarded as original biological species. One group consists of a single isolate, the other includes five strains, the remaining two groups containing the majority of isolates. Two strains of A. microstictum fell into two different original groups.},\r\ncorrespondence_address1={Mokrousov, I.V.; Chemical Pharmaceutical Institute, St. Petersburg, Russia},\r\nissn={00166758},\r\ncoden={GNKAA},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Polymorphism of yeast-like fungus Aureobasidium pullulans (De Bary) revealed by universally primed polymerase chain reaction: Species divergence state.\n \n \n \n \n\n\n \n Bulat, S.; and Mironenko, N.\n\n\n \n\n\n\n Genetika, 28(4): 19-30. 1992.\n cited By 12\n\n\n\n
\n\n\n\n \n \n $\"Polymorphism$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat199219,\r\nauthor={Bulat, S.A. and Mironenko, N.V.},\r\ntitle={Polymorphism of yeast-like fungus Aureobasidium pullulans (De Bary) revealed by universally primed polymerase chain reaction: Species divergence state},\r\njournal={Genetika},\r\nyear={1992},\r\nvolume={28},\r\nnumber={4},\r\npages={19-30},\r\nnote={cited By 12},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026701081&partnerID=40&md5=eb2f63ae13bd348227b6983b4c17a359},\r\naffiliation={St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia},\r\nabstract={Genome structure of ecologically different collection strains of Aureobasidium pullulans fungus was analysed by universally primed polymerase chain reaction (UP-PCR) technique, aimed at elucidation of their general unity. As a result, isolates of different ecological groups have shown specific UP-PCR patterns, i.e. were heterogeneous in genome structure. This reflects the divergence state of the fungus. The phytopathogenic isolates apparently represent original biological species, while other isolates being in the divergence process retained genetical unity in the different biological species terms. Common properties of UP-PCR patterns are characterized, too.},\r\ncorrespondence_address1={Bulat, S.A.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia},\r\nissn={00166758},\r\ncoden={GNKAA},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n\n\n

\n \n\n \n \n \n \n \n \n The identification of marker strains of Leishmania major, L. turanica and L. gerbilli by the polymer chain reaction with a universal primer [Identifikatsiia markernykh shtammov Leishmania major, L. turanica, L. gerbilli metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nym praǐmerom.].\n \n \n \n \n\n\n \n Bulat, S.; Strelkova, M.; and Sysoev, V.\n\n\n \n\n\n\n Meditsinskaya Parazitologiya i Parazitarnye Bolezni, (1): 21-22. 1992.\n cited By 1\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat199221,\r\nauthor={Bulat, S.A. and Strelkova, M.V. and Sysoev, V.V.},\r\ntitle={The identification of marker strains of Leishmania major, L. turanica and L. gerbilli by the polymer chain reaction with a universal primer [Identifikatsiia markernykh shtammov Leishmania major, L. turanica, L. gerbilli metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nym praǐmerom.]},\r\njournal={Meditsinskaya Parazitologiya i Parazitarnye Bolezni},\r\nyear={1992},\r\nnumber={1},\r\npages={21-22},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026444928&partnerID=40&md5=e3c764b00b00d856acd71a3db9967baa},\r\nabstract={The Leningrad Nuclear Physics Institute, Academy of Science of the USSR, and Martsinovskiǐ Institute of Medical Parasitology and Tropical Medicine, USSR Ministry of Health, developed polymerase chain reaction (PCR) technique with universal primer 3-2 for Leishmania identification. The primers were patented in the USSR (Patent No 4757254, 1989). Reference strains of three Leishmania species were identified: L. major--MRHO/SU/59/Neal P; L. gerbilli--MRHO/CN/60/gerbilli; L. turanica--MRHO/SU/80/Cl 3720 and MRHO/SU/83/KD 051. Each Leishmania species is specific and different in its PCR pattern whereas the two L. turanica strains have identical PCR patterns of the given primer.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00258326},\r\npubmed_id={1380631},\r\nlanguage={Russian},\r\nabbrev_source_title={Med Parazitol (Mosk)},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Universally primed polymerase chain reaction: Primer and genome DNA patterns characteristics.\n \n \n \n \n\n\n \n Bulat, S.; Kobaev, O.; Mironenko, N.; Ibatullin, F.; Luchkina, L.; and Suslov, A.\n\n\n \n\n\n\n Genetika, 28(5): 19-28. 1992.\n cited By 10\n\n\n\n
\n\n\n\n \n \n $\"Universally$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat199219,\r\nauthor={Bulat, S.A. and Kobaev, O.K. and Mironenko, N.V. and Ibatullin, F.M. and Luchkina, L.A. and Suslov, A.V.},\r\ntitle={Universally primed polymerase chain reaction: Primer and genome DNA patterns characteristics},\r\njournal={Genetika},\r\nyear={1992},\r\nvolume={28},\r\nnumber={5},\r\npages={19-28},\r\nnote={cited By 10},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026862795&partnerID=40&md5=d70b1c12c001879c0ca833e6e06f9501},\r\naffiliation={Leningrad Inst. of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia},\r\nabstract={Universal primer ability of generating conservative and variable UP-PCR (universally primed polymerase chain reaction) species-specific patterns was analysed on bacteria to serve as an example. Also, two important properties of the UP-PCR patterns (species/primer DNA hybridization specificity) are characterized.},\r\ncorrespondence_address1={Bulat, S.A.; Leningrad Inst. of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia},\r\nissn={00166758},\r\ncoden={GNKAA},\r\npubmed_id={1639258},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n

\n \n 1991\n \n \n (1)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n The gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of Yersinia pseudotuberculosis [Genoidentifikatsiia vidov i serovariantov bakteriǐ metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi oligonukleotidami: reidentifikatsiia ranee vydelennykh shtammov Yersinia pseudotuberculosis.].\n \n \n \n \n\n\n \n Bulat, S.; Mikhaǐlo, N.; and Koroliuk, A.\n\n\n \n\n\n\n Zhurnal Mikrobiologii Epidemiologii i Immunobiologii, (12): 2-7. 1991.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"The$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19912,\r\nauthor={Bulat, S.A. and Mikhaǐlo, N.V. and Koroliuk, A.M.},\r\ntitle={The gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of Yersinia pseudotuberculosis [Genoidentifikatsiia vidov i serovariantov bakteriǐ metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi oligonukleotidami: reidentifikatsiia ranee vydelennykh shtammov Yersinia pseudotuberculosis.]},\r\njournal={Zhurnal Mikrobiologii Epidemiologii i Immunobiologii},\r\nyear={1991},\r\nnumber={12},\r\npages={2-7},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026267998&partnerID=40&md5=887ae9e53c581a45ad04728b38e05f0c},\r\nabstract={Genetic analysis of 19 standard strains belonging to 6 Yersinia species (Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, Y. kirstensenii, Y. frederiksenii, Y. intermedia) revealed that gene typing by the method of polymerase chain reaction (PCR) with the use of universal primers permitted the identification of species in bacterial cultures by PCR patterns and the determination of Y. pseudotuberculosis serovars within 4 hours. By this method 23 Y. pseudotuberculosis strains (serovar 1), earlier isolated in different regions of the USSR from humans and rodents, were studied. The study showed that out of 14 strains of human origin only two strains could actually be classified with serovar 1, while the remaining strains were reidentified as belonging to serovar 5. Among 9 strains isolated from rodents those of serovar 1 prevailed (8 strains). The authors suppose that strains of serovar 5 cause outbreaks and sporadic cases of pseudotuberculosis, occurring considerably more often than it is commonly believed in the USSR.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={03729311},\r\npubmed_id={1789029},\r\nlanguage={Russian},\r\nabbrev_source_title={Zh Mikrobiol Epidemiol Immunobiol},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n

\n \n 1990\n \n \n (1)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n High-polyploid lines of Saccharomycetes developed by the method of destabilization of the third (sex) chromosome.\n \n \n \n \n\n\n \n Bulat, S.; Sokolova, O.; and Zakharov, I.\n\n\n \n\n\n\n Doklady Biological Sciences, 309(1-6): 746-749. 1990.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"High-polyploid$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat1990746,\r\nauthor={Bulat, S.A. and Sokolova, O.V. and Zakharov, I.A.},\r\ntitle={High-polyploid lines of Saccharomycetes developed by the method of destabilization of the third (sex) chromosome},\r\njournal={Doklady Biological Sciences},\r\nyear={1990},\r\nvolume={309},\r\nnumber={1-6},\r\npages={746-749},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025004769&partnerID=40&md5=bf8733e8159d06961edacd90cb5c3591},\r\naffiliation={B. P. Konstantinov Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Leningrad Oblast, Russia},\r\nissn={00124966},\r\ncoden={DKBSA},\r\nlanguage={English},\r\nabbrev_source_title={DOKL. BIOL. SCI.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n

\n\n\n\n\n\n

\n

\n \n 1989\n \n \n (2)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n DNA polymorphism in phytopathogenic fungus Pyrenophora tritici-repentis (Died.) Drechsler.\n \n \n \n \n\n\n \n Bulat, S.; and Mironenko, N.\n\n\n \n\n\n\n Genetika, 25(11): 2059-2063. 1989.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"DNA$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19892059,\r\nauthor={Bulat, S.A. and Mironenko, N.V.},\r\ntitle={DNA polymorphism in phytopathogenic fungus Pyrenophora tritici-repentis (Died.) Drechsler},\r\njournal={Genetika},\r\nyear={1989},\r\nvolume={25},\r\nnumber={11},\r\npages={2059-2063},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024357935&partnerID=40&md5=caf31725753213624d445eb4773122cb},\r\naffiliation={B.P. Konstantinov Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Leningrad, Russia},\r\nissn={00166758},\r\ncoden={GNKAA},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Highly polyploid lines of saccharomycete yeasts produced by destabilization of chromosome III (sexual) [Vysokopoliploidnye linii drozhzheǐ-sakharomitsetov, vyvedennye metodom destabilizatsii III (polovoǐ) khromosomy.].\n \n \n \n \n\n\n \n Bulat, S.; Sokolova, O.; and Zakharov, I.\n\n\n \n\n\n\n Doklady Akademii nauk SSSR, 309(5): 1230-1233. 1989.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Highly$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19891230,\r\nauthor={Bulat, S.A. and Sokolova, O.V. and Zakharov, I.A.},\r\ntitle={Highly polyploid lines of saccharomycete yeasts produced by destabilization of chromosome III (sexual) [Vysokopoliploidnye linii drozhzheǐ-sakharomitsetov, vyvedennye metodom destabilizatsii III (polovoǐ) khromosomy.]},\r\njournal={Doklady Akademii nauk SSSR},\r\nyear={1989},\r\nvolume={309},\r\nnumber={5},\r\npages={1230-1233},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024834948&partnerID=40&md5=a076f60c0cc2487dfebef8d6d522e15b},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00023264},\r\npubmed_id={2632208},\r\nlanguage={Russian},\r\nabbrev_source_title={Dokl Akad Nauk SSSR},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n

\n\n\n\n\n\n

\n

\n \n 1987\n \n \n (5)\n \n \n

\n

\n \n \n

\n \n\n \n \n \n \n \n \n Genetic study of plasmid integration into yeast chromosomes. VI. Patterns of the destabilization of chimeric chromosomes and their use in gene mapping [Geneticheskoe izuchenie integratsii plazmid v drozhzhevye khromosomy. Soobshchenie VI. Zakonomernosti destabilizatsii khimernykh khromosom i ikh ispol'zovanie dlia kartirovaniia genov.].\n \n \n \n \n\n\n \n Bulat, S.\n\n\n \n\n\n\n Genetika, 23(12): 2138-2147. 1987.\n cited By 2\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19872138,\r\nauthor={Bulat, S.A.},\r\ntitle={Genetic study of plasmid integration into yeast chromosomes. VI. Patterns of the destabilization of chimeric chromosomes and their use in gene mapping [Geneticheskoe izuchenie integratsii plazmid v drozhzhevye khromosomy. Soobshchenie VI. Zakonomernosti destabilizatsii khimernykh khromosom i ikh ispol'zovanie dlia kartirovaniia genov.]},\r\njournal={Genetika},\r\nyear={1987},\r\nvolume={23},\r\nnumber={12},\r\npages={2138-2147},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023512812&partnerID=40&md5=1a100f0088b1537f274afd1eb0e41eef},\r\nabstract={The 2 microns DNA-dependent destabilization of yeast chimeric chromosomes III, IV, V was analysed. The comparison of its peculiarities with the earlier localized sites of episomal plasmid integration allowed to derive genetic regularities of destabilization process. Two destabilization rules that describe patterns of the loss of genetic information in the chromosome were formulated. The usefulness of this for mitotic intrachromosomal gene mapping in yeast was demonstrated using plasmid integration site mapping in chromosome I.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00166758},\r\npubmed_id={3326784},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Saccharomyces cerevisiae mutants characterized by increased induced mutagenesis. II. Genetic analysis of mutants [Mutanty drozhzheǐ Saccharomyces cerevisiae, kharakterizuiushchiesia povyshennym indutsirovannym mutagenezom. Soobshchenie II. Geneticheskiǐ analiz mutantov.].\n \n \n \n \n\n\n \n Ivanov, E.; Bulat, S.; Korolev, V.; and Koval'tsova, S.\n\n\n \n\n\n\n Genetika, 23(8): 1383-1389. 1987.\n cited By 3\n\n\n\n
\n\n\n\n \n \n $\"Saccharomyces$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Ivanov19871383,\r\nauthor={Ivanov, E.L. and Bulat, S.A. and Korolev, V.G. and Koval'tsova, S.V.},\r\ntitle={Saccharomyces cerevisiae mutants characterized by increased induced mutagenesis. II. Genetic analysis of mutants [Mutanty drozhzheǐ Saccharomyces cerevisiae, kharakterizuiushchiesia povyshennym indutsirovannym mutagenezom. Soobshchenie II. Geneticheskiǐ analiz mutantov.]},\r\njournal={Genetika},\r\nyear={1987},\r\nvolume={23},\r\nnumber={8},\r\npages={1383-1389},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023390937&partnerID=40&md5=0ba8da97a6b9b62d21d085d7af699537},\r\nabstract={Induction of forward adenine-dependent (Ade+----Ade-) mutations by HAP was used to analyse genetically yeast mutants with enhanced induced mutagenesis. Three mutations studied in detail segregated as a single mendelian trait and composed independent complementation groups (HIM1, HIM2, HIM3). the him1-1 mutation was centromere-linked, the him3-1 and him2-1 mutations being not. All three mutations did not show any cross-linkage. Uracil-DNA glycosylase activity was determined in crude cell extract from wild type strain and him mutants; no detectable differences were observed.},\r\ncorrespondence_address1={Ivanov, E.L.},\r\nissn={00166758},\r\npubmed_id={3311878},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91 [Geneticheskoe izuchenie integratsii plazmid v drozhzhevye khromosomy. Soobshchenie V. Kartirovanie saǐtov integratsii plazmidy pYF91.].\n \n \n \n \n\n\n \n Bulat, S.; Mezhevaia, E.; Stepanova, V.; Iarovoǐ, B.; and Zakharov, I.\n\n\n \n\n\n\n Genetika, 23(8): 1407-1413. 1987.\n cited By 1\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat19871407,\r\nauthor={Bulat, S.A. and Mezhevaia, E.V. and Stepanova, V.P. and Iarovoǐ, B.F. and Zakharov, I.A.},\r\ntitle={Genetic study of plasmid integration into yeast chromosomes. V. Mapping of integration sites for the plasmid pYF91 [Geneticheskoe izuchenie integratsii plazmid v drozhzhevye khromosomy. Soobshchenie V. Kartirovanie saǐtov integratsii plazmidy pYF91.]},\r\njournal={Genetika},\r\nyear={1987},\r\nvolume={23},\r\nnumber={8},\r\npages={1407-1413},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023395886&partnerID=40&md5=32d47a361cc580c083f3f4210610fecd},\r\nabstract={The data on mapping the episomal plasmid integration sites in yeast chromosomes I, III, IV, V, VII, XV are presented. In addition to the integration site at leu2 of chromosome III localized earlier, 6 more loci containing apparently the homologous yeast transposons, with a copy in a plasmid, were defined. The fact of plasmid integration was proved by colony hybridization technique with the pBR322 probe. The plasmid DNA segregation (the ratio 2:2) and its linkage to pLEU2 plasmid marker gene were observed in hybrids of all integrants studied.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00166758},\r\npubmed_id={3311880},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Genetic study of plasmid integration into the yeast chromosomes. III. Phenotypic detection and genetic testing of the yeast Saccharomyces cerevisiae (Meyen ex Hansen) clones with lost 2μ DNA.\n \n \n \n \n\n\n \n Bulat, S.; and Mezhevaya, E.\n\n\n \n\n\n\n Genetika, 23(3): 421-430. 1987.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{Bulat1987421,\r\nauthor={Bulat, S.A. and Mezhevaya, E.V.},\r\ntitle={Genetic study of plasmid integration into the yeast chromosomes. III. Phenotypic detection and genetic testing of the yeast Saccharomyces cerevisiae (Meyen ex Hansen) clones with lost 2μ DNA},\r\njournal={Genetika},\r\nyear={1987},\r\nvolume={23},\r\nnumber={3},\r\npages={421-430},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023304901&partnerID=40&md5=01f13778a40077bcff43d37523761fb2},\r\naffiliation={B.P. Konstantinov Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Russian Federation},\r\nissn={00166758},\r\ncoden={GNKAA},\r\npubmed_id={3552879},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

\n

\n\n\n\n

\n\n\n

\n \n\n \n \n \n \n \n \n Genetic study of plasmid integration into the yeast chromosomes. IV. Integration of plasmid pYF91 into different yeast chromosomes.\n \n \n \n \n\n\n \n Yarovoy Ph., B.; Stepnaova, V.; and Bulat, S.\n\n\n \n\n\n\n Genetika, 23(3): 431-439. 1987.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"Genetic$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

\n

@ARTICLE{YarovoyPh.1987431,\r\nauthor={Yarovoy Ph., B. and Stepnaova, V.P. and Bulat, S.A.},\r\ntitle={Genetic study of plasmid integration into the yeast chromosomes. IV. Integration of plasmid pYF91 into different yeast chromosomes},\r\njournal={Genetika},\r\nyear={1987},\r\nvolume={23},\r\nnumber={3},\r\npages={431-439},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023302451&partnerID=40&md5=91cd3ce303ff21d7ad5b4eecbf252c43},\r\naffiliation={B.P. Konstantinov Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Russian Federation},\r\nissn={00166758},\r\ncoden={GNKAA},\r\npubmed_id={3552880},\r\nlanguage={Russian},\r\nabbrev_source_title={GENETIKA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n 1985\n \n \n (1)\n \n \n

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\n \n\n \n \n \n \n \n \n Cloning of a yeast gene \"in vivo\" and its transfer as a part of an autonomously replicating unit: gene seduction.\n \n \n \n \n\n\n \n Bulat, S.; Peshekhonov, V.; Chepurnaya, O.; and Zakharov, I.\n\n\n \n\n\n\n Current Genetics, 9(2): 119-121. 1985.\n cited By 2\n\n\n\n
\n\n\n\n \n \n $\"Cloning$ Paper\n \n \n\n \n \n doi\n \n \n\n \n link\n \n \n\n bibtex\n \n\n \n \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat1985119,\r\nauthor={Bulat, S.A. and Peshekhonov, V.T. and Chepurnaya, O.V. and Zakharov, I.A.},\r\ntitle={Cloning of a yeast gene "in vivo" and its transfer as a part of an autonomously replicating unit: gene seduction},\r\njournal={Current Genetics},\r\nyear={1985},\r\nvolume={9},\r\nnumber={2},\r\npages={119-121},\r\ndoi={10.1007/BF00436958},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021912460&doi=10.1007%2fBF00436958&partnerID=40&md5=ae8f6b33cfcdce1a84aee6c953be484b},\r\naffiliation={Laboratory of Molecular and Radiation Biophysics, B. P. Konstantinov Leningrad Institut of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Russia},\r\nabstract={Yeast diploids containing a chromosomally integrated episomal plasmid manifest the mitotic instability of the chromosomes with the plasmid. Probably as a results of the destabilization integrated plasmid may be excised out of the chromosome. It was found that the rescue of the plasmid may be irregular, with the taking up of adjacent chromosomal gene. Using an integrant with the plasmid integrated into chromosome I very near the ADE1 locus we cloned this gene "in vivo" by selecting rescued plasmid marker LEU2. The new plasmid has retained the LEU2 gene and the capacity to replicate autonomously. This plasmid was used to transfer the cloned ADEI gene from one cell to another by transformation and from one resident chromosome to another by integration. The phenomenon of irregular excision of an integrated episomal plasmid together with linked chromosomal gene(s) and transfer to other chromosomes or cells we propose to term Seduction. © 1985 Springer-Verlag.},\r\nauthor_keywords={Chromosomal integration;  Episomal plasmid;  Gene seduction;  Plasmid rescue},\r\ncorrespondence_address1={Bulat, S.A.; Laboratory of Molecular and Radiation Biophysics, B. P. Konstantinov Leningrad Institut of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Russia},\r\npublisher={Springer-Verlag},\r\nissn={01728083},\r\ncoden={CUGED},\r\nlanguage={English},\r\nabbrev_source_title={Curr Genet},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n

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\n \n\n \n \n \n \n \n \n In vivo cloning of a yeast gene and its transfer with an autonomously replicating element: The gene seduction.\n \n \n \n \n\n\n \n Bulat, S.; Peshekhonov, V.; Chepurnaya, O.; and Zakharov, I.\n\n\n \n\n\n\n Doklady Biological Sciences, 277(1-6): 461-464. 1984.\n cited By 0\n\n\n\n
\n\n\n\n \n \n $\"In$ Paper\n \n \n\n \n\n \n link\n \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n \n \n \n\n\n\n

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@ARTICLE{Bulat1984461,\r\nauthor={Bulat, S.A. and Peshekhonov, V.T. and Chepurnaya, O.V. and Zakharov, I.A.},\r\ntitle={In vivo cloning of a yeast gene and its transfer with an autonomously replicating element: The gene seduction},\r\njournal={Doklady Biological Sciences},\r\nyear={1984},\r\nvolume={277},\r\nnumber={1-6},\r\npages={461-464},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021733258&partnerID=40&md5=be079d69ea80be916a03661951eb31ee},\r\naffiliation={B.P. Konstantinov Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Leningrad, Russia},\r\nissn={00124966},\r\ncoden={DKBSA},\r\nlanguage={English},\r\nabbrev_source_title={DOKL. BIOL. SCI.},\r\nsource={Scopus},\r\n}\r\n

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