var bibbase_data = {"data":"<img src=\"//bibbase.org/img/ajax-loader.gif\" id=\"spinner\"\n  style=\"display: none;\" alt=\"Loading..\" />\n\n<div id=\"bibbase\">\n\n  <script type=\"text/javascript\">\n    var bibbase = {\n      params: {\"bib\":\"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lkb_2019_10.txt\",\"jsonp\":\"1\",\"host\":\"bibbase.org\"},\n      data: [{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zabrodskaya\"],\"firstnames\":[\"Y.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pipich\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kuklin\"],\"firstnames\":[\"A.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ramsay\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sokolov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Elizarova\"],\"firstnames\":[\"A.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shaldzhyan\"],\"firstnames\":[\"A.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grudinina\"],\"firstnames\":[\"N.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Wu\"],\"firstnames\":[\"B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volnitskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Schmidt\"],\"firstnames\":[\"A.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shvetsov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vasilyev\"],\"firstnames\":[\"V.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Effect of alpha-lactalbumin and lactoferrin oleic acid complexes on chromatin structural organization\",\"journal\":\"Biochemical and Biophysical Research Communications\",\"year\":\"2019\",\"volume\":\"520\",\"number\":\"1\",\"pages\":\"136-139\",\"doi\":\"10.1016/j.bbrc.2019.09.116\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&doi=10.1016%2fj.bbrc.2019.09.116&partnerID=40&md5=a06fb06bdcf8a8d38c05dce20a1ca016\",\"affiliation\":\"Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B. P. Konstantinov of National Research Center “Kurchatov Institute”, mkr. Orlova roshcha 1, Gatchina, 188300, Russian Federation; National Research Centre Kurchatov Institute, Akademika Kurchatova Sq. 1, Moscow, 123182, Russian Federation; Peter the Great Saint-Petersburg Polytechnic University, Politekhnicheskaya 29, St. Petersburg, 194064, Russian Federation; Department of Molecular Biology of Viruses, Smorodintsev Research Institute of Influenza, Russian Ministry of Health, Prof. Popov St. 15/17, St. Petersburg, 197376, Russian Federation; Jülich Centre for Neutron Science at Heinz Maier-Leibnitz Zentrum, Lichtenbergstr. 1, Garching, Munich D-85747, Germany; Frank Laboratory of Neutron Physics, International Intergovernmental Organization Joint Institute for Nuclear Research, Joliot-Curie St. 6, Moscow Region, Dubna, 141980, Russian Federation; Moscow Institute of Physics and Technology (State University), Institutskiy per. 9, Dolgoprudny, Moscow Region 141701, Russian Federation; Department of Molecular Genetics, Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”, Akademika Pavlova St. 12, St. Petersburg, 197376, Russian Federation; St. Petersburg University, Universitetskaya Emb. 7-9, St Petersburg, 199034, Russian Federation\",\"abstract\":\"This work focuses on the study of multimeric alpha-lactalbumin oleic acid and lactoferrin oleic acid complexes. The purpose of the research is to study possible mechanisms involved in their pro-apoptotic activities, as seen in some tumor cell cultures. Complexes featuring oleic acid (OA) with human alpha-lactalbumin (hAl) or with bovine alpha-lactalbumin (bAl), and human lactoferrin (hLf) were investigated using small-angle neutron scattering (SANS). It was shown that while alpha-lactalbumin protein complexes were formed on the surface of polydisperse OA micelles, the lactoferrin complexes comprised a monodisperse system of nanoscale particles. Both hAl and hLf complexes appeared to interact with the chromatin of isolated nuclei affecting chromatin structural organization. The possible roles of these processes in the specific anti-tumor activity of these complexes are discussed. © 2019 Elsevier Inc.\",\"author_keywords\":\"Antitumor agents; Chromatin structural organization; Lactalbumin; Lactoferrin; Oleic acid\",\"funding_details\":\"National Rehabilitation Center1363\",\"key\":\"Lebedev2019136\",\"id\":\"Lebedev2019136\",\"bibbaseid\":\"anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&doi=10.1016%2fj.bbrc.2019.09.116&partnerID=40&md5=a06fb06bdcf8a8d38c05dce20a1ca016\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":4,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bolshakova\"],\"firstnames\":[\"O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Borisenkova\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Suyasova\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sedov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slobodina\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Timoshenko\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Golomidov\"],\"firstnames\":[\"I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Aksenov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sarantseva\"],\"firstnames\":[\"S.\"],\"suffixes\":[]}],\"title\":\"In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method\",\"journal\":\"Materials Science and Engineering C\",\"year\":\"2019\",\"volume\":\"104\",\"doi\":\"10.1016/j.msec.2019.109945\",\"art_number\":\"109945\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&doi=10.1016%2fj.msec.2019.109945&partnerID=40&md5=71eb20737220e772bd8bc01af8589292\",\"affiliation\":\"Petersburg Nuclear Physics Institute, National Research Centre “Kurchatov Institute, Gatchina, 188300, Russian Federation; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, 141980, Russian Federation\",\"abstract\":\"The toxicity of C60(OH)30, C70(OH)30, and C120O(OH)n fullerenols, prepared by a new original method, has been studied. This method allowed us to obtain high-purity fullerenols and eliminate the risks of synthesis of preparations containing insoluble fractions contaminated with impurities such as fullerenes not completely reacted by hydroxylation. All fullerenols were detected inside cultured cells. The MTT assay as well as the analysis of apoptosis and cell cycle showed that С60(ОН)30 and С70(OH)30 are non-toxic for cultured V79 и HeLa cells at concentrations exceeding physiological levels by an order of magnitude. С120O(OH)n caused low toxicity. Studies in Drosophila melanogaster showed that any preparations used did not result in a decreased lifespan or in behavior abnormalities in flies. © 2019 Elsevier B.V.\",\"author_keywords\":\"Cell culture; Drosophila melanogaster; Fullerenols; Toxicity\",\"funding_details\":\"15-04-99647\",\"key\":\"Bolshakova2019\",\"id\":\"Bolshakova2019\",\"bibbaseid\":\"anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&doi=10.1016%2fj.msec.2019.109945&partnerID=40&md5=71eb20737220e772bd8bc01af8589292\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":4,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Iashina\"],\"firstnames\":[\"E.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bouwman\"],\"firstnames\":[\"W.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Duif\"],\"firstnames\":[\"C.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Honecker\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pipich\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grigoriev\"],\"firstnames\":[\"S.V.\"],\"suffixes\":[]}],\"title\":\"Small-angle neutron scattering (SANS) and spinecho SANS measurements reveal the logarithmic fractal structure of the large-scale chromatin organization in HeLa nuclei\",\"journal\":\"Journal of Applied Crystallography\",\"year\":\"2019\",\"volume\":\"52\",\"number\":\"4\",\"pages\":\"844-853\",\"doi\":\"10.1107/S160057671900921X\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&doi=10.1107%2fS160057671900921X&partnerID=40&md5=3e50710e2425c6cdf7917c01d498f65d\",\"affiliation\":\"Saint Petersburg State University, Ulyanovskaya 1, Saint Petersburg, 198504, Russian Federation; Petersburg Nuclear Physics Institute NRC, Kurchatov Institute, Gatchina, Saint Petersburg, 188300, Russian Federation; Delft University of Technology, Mekelweg 15, Delft, 2629 JB, Netherlands; Institut Laue-Langevin, Cedex 9, Grenoble, F-38042, France; Heinz Maier-Leibnitz Zentrum, Lichtenbergstrasse 1, Garching, 85747, Germany\",\"abstract\":\"This paper reports on the two-scale fractal structure of chromatin organization in the nucleus of the HeLa cell. Two neutron scattering methods, small-angle neutron scattering (SANS) and spin-echo SANS, are used to unambiguously identify the large-scale structure as being a logarithmic fractal with the correlation function (r) - ln(r/E). The smaller-scale structural level is shown to be a volume fractal with dimension DF = 2.41. By definition, the volume fractal is self-similar at different scales, while the logarithmic fractal is hierarchically changed upon scaling. As a result, the logarithmic fractal is more compact than the volume fractal but still has a rather high surface area, which provides accessibility at all length scales. Apparently such bi-fractal chromatin organization is the result of an evolutionary process of optimizing the compactness and accessibility of gene packing. As they are in a water solution, the HeLa nuclei tend to agglomerate over time. The large-scale logarithmic fractal structure of chromatin provides the HeLa nucleus with the possibility of penetrating deeply into the adjacent nucleus during the agglomeration process. The interpenetration phenomenon of the HeLa nuclei shows that the chromatin-free space of one nucleus is not negligible but is as large as the volume occupied by chromatin itself. It is speculated that it is the logarithmic fractal architecture of chromatin that provides a comfortable compartment for this most important function of the cell. © 2019 International Union of Crystallography.\",\"author_keywords\":\"Chromatin; DNA; Fractals; Small-angle neutron scattering; Spinecho small-angle neutron scattering\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)17-02-00313 A\",\"key\":\"Iashina2019844\",\"id\":\"Iashina2019844\",\"bibbaseid\":\"anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&doi=10.1107%2fS160057671900921X&partnerID=40&md5=3e50710e2425c6cdf7917c01d498f65d\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bayramukov\"],\"firstnames\":[\"V.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2019\",\"volume\":\"13\",\"number\":\"3\",\"pages\":\"188-197\",\"doi\":\"10.1134/S1990519X1903009X\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&doi=10.1134%2fS1990519X1903009X&partnerID=40&md5=89a5f8a9f3aba34d2c3fe326d4ff81c1\",\"affiliation\":\"St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, 191036, Russian Federation\",\"abstract\":\"Abstract: In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population. © 2019, Pleiades Publishing, Ltd.\",\"author_keywords\":\"cell-to-cell communication; exosomes; Hek293 cell line; p53-GFP\",\"correspondence_address1\":\"Filatov, M.V.; St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov InstituteRussian Federation; email: fil_53@mail.ru\",\"publisher\":\"Pleiades Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Pantina2019188,\\r\\nauthor={Pantina, R.A. and Varfolomeeva, E.Y. and Burdakov, V.S. and Landa, S.B. and Bayramukov, V.Y. and Kovalev, R.A. and Filatov, M.V.},\\r\\ntitle={Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2019},\\r\\nvolume={13},\\r\\nnumber={3},\\r\\npages={188-197},\\r\\ndoi={10.1134/S1990519X1903009X},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&doi=10.1134%2fS1990519X1903009X&partnerID=40&md5=89a5f8a9f3aba34d2c3fe326d4ff81c1},\\r\\naffiliation={St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, 191036, Russian Federation},\\r\\nabstract={Abstract: In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population. © 2019, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={cell-to-cell communication;  exosomes;  Hek293 cell line;  p53-GFP},\\r\\ncorrespondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov InstituteRussian Federation; email: fil_53@mail.ru},\\r\\npublisher={Pleiades Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Pantina, R.\",\"Varfolomeeva, E.\",\"Burdakov, V.\",\"Landa, S.\",\"Bayramukov, V.\",\"Kovalev, R.\",\"Filatov, M.\"],\"key\":\"Pantina2019188\",\"id\":\"Pantina2019188\",\"bibbaseid\":\"pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&doi=10.1134%2fS1990519X1903009X&partnerID=40&md5=89a5f8a9f3aba34d2c3fe326d4ff81c1\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Volnitskiy\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konev\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]}],\"title\":\"Abnormal activity of transcription factors gli in high-grade gliomas\",\"journal\":\"PLoS ONE\",\"year\":\"2019\",\"volume\":\"14\",\"number\":\"2\",\"doi\":\"10.1371/journal.pone.0211980\",\"art_number\":\"e0211980\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&doi=10.1371%2fjournal.pone.0211980&partnerID=40&md5=b33be3ba1c58420838f9b1c65e11c6ae\",\"affiliation\":\"Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre \\\"Kurchatov Institute, Gatchina, Russian Federation; N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Pesochnyj, Leningradskaya, Russian Federation\",\"abstract\":\"Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity of transcription factors gli in high-grade gliomas and their role in maintenance of stem cell state and glioma cell survival. 20 glioma cell lines and a sample of a normal adult brain tissue were used in the present study. We found the expression of gli target genes, including GLI1 and FOXM1, in all tested glioma cell lines, but not in the normal tissue. Interestingly, the expression of gli target genes in some glioma cell lines was observed together with a high level of their transcriptional repressor, Gli3R. Knockdown of GLI3 in one of these lines resulted in decrease of gli target gene expression. These data suggest that Gli3R does not prevent the gli target genes transcription, and gli3 acts in glioma cells more as an activator, than a repressor of transcription. We observed that gli regulated the expression of such genes, as SOX2 or OCT4 that maintain stem cell state, and TET1, involving in DNA demethylation. Treatment with GANT61 or siRNA against GLI1, GLI2, or GLI3 could result in complete glioma cell death, while cyclopamine had a weaker and line-specific effect on glioma cell survival. Thus, the gli transcription factors are abnormally active in high-grade gliomas, regulate expression of genes, maintaining the stem cell state, and contribute to glioma cell survival. © 2019 Volnitskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.\",\"correspondence_address1\":\"Volnitskiy, A.; Petersburg Nuclear Physics Institute named by B.P. Konstantinov, National Research Centre \\\"Kurchatov InstituteRussian Federation; email: voln.a@yandex.ru\",\"publisher\":\"Public Library of Science\",\"issn\":\"19326203\",\"coden\":\"POLNC\",\"pubmed_id\":\"30730955\",\"language\":\"English\",\"abbrev_source_title\":\"PLoS ONE\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Volnitskiy2019,\\r\\nauthor={Volnitskiy, A. and Shtam, T. and Burdakov, V. and Kovalev, R. and Konev, A. and Filatov, M.},\\r\\ntitle={Abnormal activity of transcription factors gli in high-grade gliomas},\\r\\njournal={PLoS ONE},\\r\\nyear={2019},\\r\\nvolume={14},\\r\\nnumber={2},\\r\\ndoi={10.1371/journal.pone.0211980},\\r\\nart_number={e0211980},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&doi=10.1371%2fjournal.pone.0211980&partnerID=40&md5=b33be3ba1c58420838f9b1c65e11c6ae},\\r\\naffiliation={Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre \\\"Kurchatov Institute, Gatchina, Russian Federation; N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Pesochnyj, Leningradskaya, Russian Federation},\\r\\nabstract={Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity of transcription factors gli in high-grade gliomas and their role in maintenance of stem cell state and glioma cell survival. 20 glioma cell lines and a sample of a normal adult brain tissue were used in the present study. We found the expression of gli target genes, including GLI1 and FOXM1, in all tested glioma cell lines, but not in the normal tissue. Interestingly, the expression of gli target genes in some glioma cell lines was observed together with a high level of their transcriptional repressor, Gli3R. Knockdown of GLI3 in one of these lines resulted in decrease of gli target gene expression. These data suggest that Gli3R does not prevent the gli target genes transcription, and gli3 acts in glioma cells more as an activator, than a repressor of transcription. We observed that gli regulated the expression of such genes, as SOX2 or OCT4 that maintain stem cell state, and TET1, involving in DNA demethylation. Treatment with GANT61 or siRNA against GLI1, GLI2, or GLI3 could result in complete glioma cell death, while cyclopamine had a weaker and line-specific effect on glioma cell survival. Thus, the gli transcription factors are abnormally active in high-grade gliomas, regulate expression of genes, maintaining the stem cell state, and contribute to glioma cell survival. © 2019 Volnitskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},\\r\\ncorrespondence_address1={Volnitskiy, A.; Petersburg Nuclear Physics Institute named by B.P. Konstantinov, National Research Centre \\\"Kurchatov InstituteRussian Federation; email: voln.a@yandex.ru},\\r\\npublisher={Public Library of Science},\\r\\nissn={19326203},\\r\\ncoden={POLNC},\\r\\npubmed_id={30730955},\\r\\nlanguage={English},\\r\\nabbrev_source_title={PLoS ONE},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Volnitskiy, A.\",\"Shtam, T.\",\"Burdakov, V.\",\"Kovalev, R.\",\"Konev, A.\",\"Filatov, M.\"],\"key\":\"Volnitskiy2019\",\"id\":\"Volnitskiy2019\",\"bibbaseid\":\"volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&doi=10.1371%2fjournal.pone.0211980&partnerID=40&md5=b33be3ba1c58420838f9b1c65e11c6ae\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":1,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Zinchenko\"],\"firstnames\":[\"Yu.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Starshinova\"],\"firstnames\":[\"A.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Denisova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yablonskiy\"],\"firstnames\":[\"P.K.\"],\"suffixes\":[]}],\"title\":\"Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ]\",\"journal\":\"Medical Immunology (Russia)\",\"year\":\"2019\",\"volume\":\"21\",\"number\":\"3\",\"pages\":\"479-486\",\"doi\":\"10.15789/1563-0625-2019-3-479-486\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&doi=10.15789%2f1563-0625-2019-3-479-486&partnerID=40&md5=3491db5f8ebdda1e86d4978600a5e9e7\",\"affiliation\":\"St. Petersburg State Research Institute of Phthisiopulmonology, St. Petersburg, Russian Federation; St. Petersburg B. Konstantinov Institute of Nuclear Physics, National Research Center “Kurchatov Institute, Gatchina, Leningrad Region, Russian Federation; St. Petersburg State University, St. Petersburg, Russian Federation\",\"abstract\":\"The etiology of sarcoidosis is not completely understood. A hypothesis exists about the relationship between sarcoidosis and a complex of pathological autoimmune reactions that occur under the influence of triggering factors. In this study, specific immune complexes in the blood plasma of patients have been determined, which can indirectly reveal the causes of the disease. The study included 33 patients with lung sarcoidosis (I group), compared to 24 healthy donors who served as a control group (II group). The patients underwent standard examination. Their blood plasma was investigated by the dynamic light scattering method with addition of tuberculosis antigens (ESAT-6/SFP-10) and “lung healthy tissue extract”. Statistical analysis was performed using the Statistica 7.0 program. Test results were considered significant at p < 0.05. Аccording to the data obtained, addition of ESAT-6/SFP-10 to patient’s blood plasma almost did not lead to the formation of immune complexes in most samples. Meanwhile, development of such complexes after addition of “lung tissue extract” was revealed in all the patients. The immune complexes were not detected in any donor from control group after stimulation with both kinds of antigens (p < 0.01). The data on distinct formation of immune complexes with the addition of “lung healthy tissue extract” in patients with lung sarcoidosis may be considered an indirect evidence for occurrence of autoimmune reaction under the influence of some pathogenic factors. Absence of de novo immune complex formation after addition of tuberculosis antigens (ESAT-6/SFP-10) makes it unlikely any direct effects of tuberculosis bacteria upon development of sarcoidosis. © 2019, SPb RAACI.\",\"author_keywords\":\"Autoimmunity; Dynamic light scattering method; Sarcoidosis; Specific immune complexes\",\"correspondence_address1\":\"Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru\",\"publisher\":\"Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)\",\"issn\":\"15630625\",\"language\":\"Russian\",\"abbrev_source_title\":\"Med. Immunol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Zinchenko2019479,\\r\\nauthor={Zinchenko, Yu.S. and Starshinova, A.A. and Filatov, M.V. and Denisova, N.V. and Landa, S.B. and Burdakov, V.S. and Yablonskiy, P.K.},\\r\\ntitle={Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ]},\\r\\njournal={Medical Immunology (Russia)},\\r\\nyear={2019},\\r\\nvolume={21},\\r\\nnumber={3},\\r\\npages={479-486},\\r\\ndoi={10.15789/1563-0625-2019-3-479-486},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&doi=10.15789%2f1563-0625-2019-3-479-486&partnerID=40&md5=3491db5f8ebdda1e86d4978600a5e9e7},\\r\\naffiliation={St. Petersburg State Research Institute of Phthisiopulmonology, St. Petersburg, Russian Federation; St. Petersburg B. Konstantinov Institute of Nuclear Physics, National Research Center “Kurchatov Institute, Gatchina, Leningrad Region, Russian Federation; St. Petersburg State University, St. Petersburg, Russian Federation},\\r\\nabstract={The etiology of sarcoidosis is not completely understood. A hypothesis exists about the relationship between sarcoidosis and a complex of pathological autoimmune reactions that occur under the influence of triggering factors. In this study, specific immune complexes in the blood plasma of patients have been determined, which can indirectly reveal the causes of the disease. The study included 33 patients with lung sarcoidosis (I group), compared to 24 healthy donors who served as a control group (II group). The patients underwent standard examination. Their blood plasma was investigated by the dynamic light scattering method with addition of tuberculosis antigens (ESAT-6/SFP-10) and “lung healthy tissue extract”. Statistical analysis was performed using the Statistica 7.0 program. Test results were considered significant at p < 0.05. Аccording to the data obtained, addition of ESAT-6/SFP-10 to patient’s blood plasma almost did not lead to the formation of immune complexes in most samples. Meanwhile, development of such complexes after addition of “lung tissue extract” was revealed in all the patients. The immune complexes were not detected in any donor from control group after stimulation with both kinds of antigens (p < 0.01). The data on distinct formation of immune complexes with the addition of “lung healthy tissue extract” in patients with lung sarcoidosis may be considered an indirect evidence for occurrence of autoimmune reaction under the influence of some pathogenic factors. Absence of de novo immune complex formation after addition of tuberculosis antigens (ESAT-6/SFP-10) makes it unlikely any direct effects of tuberculosis bacteria upon development of sarcoidosis. © 2019, SPb RAACI.},\\r\\nauthor_keywords={Autoimmunity;  Dynamic light scattering method;  Sarcoidosis;  Specific immune complexes},\\r\\ncorrespondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},\\r\\npublisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},\\r\\nissn={15630625},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Med. Immunol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Zinchenko, Y.\",\"Starshinova, A.\",\"Filatov, M.\",\"Denisova, N.\",\"Landa, S.\",\"Burdakov, V.\",\"Yablonskiy, P.\"],\"key\":\"Zinchenko2019479\",\"id\":\"Zinchenko2019479\",\"bibbaseid\":\"zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&doi=10.15789%2f1563-0625-2019-3-479-486&partnerID=40&md5=3491db5f8ebdda1e86d4978600a5e9e7\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Starshinova\"],\"firstnames\":[\"A.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Istomina\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zinchenko\"],\"firstnames\":[\"Yu.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Belyaeva\"],\"firstnames\":[\"E.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nazarenko\"],\"firstnames\":[\"M.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sapozhnikova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yablonkiy\"],\"firstnames\":[\"P.K.\"],\"suffixes\":[]}],\"title\":\"Diagnostic value of specific immune complexes in detection of active tuberculosis infection\",\"journal\":\"Medical Immunology (Russia)\",\"year\":\"2019\",\"volume\":\"21\",\"number\":\"2\",\"pages\":\"269-278\",\"doi\":\"10.15789/1563-0625-2019-2-269-278\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&doi=10.15789%2f1563-0625-2019-2-269-278&partnerID=40&md5=a90c16d4007b2884eb6918641062312c\",\"affiliation\":\"St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, St. Petersburg, 191036, Russian Federation\",\"abstract\":\"The diagnostics of tuberculosis infection, including immunological methods, evolved significant changes over time. Introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to differentiate between these conditions, thus requiring development and implementation of new diagnostic approaches. A prospective study enrolling two groups of patients was conducted as follows: group I (n = 50) included patients with verified pulmonary tuberculosis, MBT (+), whereas group II (n = 15) consisted of subjects with LTI, and control group was represented by healthy subjects (n = 14). The entire examination protocol included clinical, radiological, bacteriological, and immunological methods (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskin test). Immune complexes were determined in all patients and healthy individuals by means of dynamic light scattering after the in vitro addition of specific antigens (ESAT-6 and SFP-10 peptides). The data obtained have shown low informativity of clinical methods in diagnostics of pulmonary tuberculosis. In the presence of characteristic X-radiographic changes, bacteriological verification of tuberculosis was proven only in 46% of cases. Usage of various immunological tests allowed to obtain positive results in 84-90% of cases, along with 100% positivity in subjects with LTI. Detection of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases, and to identify the high-risk group for development of active tuberculosis among the subjects with latent tuberculosis infection. Conclusions: the obtained data may be applied both for diagnosis of active tuberculosis in the absence of verified diagnosis, but also enable identification of a high-risk group for development of the disease in subjects with latent tuberculosis infection. © 2019, SPb RAACI.\",\"author_keywords\":\"Dynamic light scattering method; Immunological tests; Latent tuberculosis infection; Test with allergen tuberculosis recombinant; Tuberculosis\",\"correspondence_address1\":\"Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru\",\"publisher\":\"Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)\",\"issn\":\"15630625\",\"language\":\"Russian\",\"abbrev_source_title\":\"Med. Immunol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Starshinova2019269,\\r\\nauthor={Starshinova, A.A. and Istomina, E.V. and Zinchenko, Yu.S. and Filatov, M.V. and Landa, S.B. and Burdakov, V.S. and Belyaeva, E.N. and Nazarenko, M.M. and Sapozhnikova, N.V. and Yablonkiy, P.K.},\\r\\ntitle={Diagnostic value of specific immune complexes in detection of active tuberculosis infection},\\r\\njournal={Medical Immunology (Russia)},\\r\\nyear={2019},\\r\\nvolume={21},\\r\\nnumber={2},\\r\\npages={269-278},\\r\\ndoi={10.15789/1563-0625-2019-2-269-278},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&doi=10.15789%2f1563-0625-2019-2-269-278&partnerID=40&md5=a90c16d4007b2884eb6918641062312c},\\r\\naffiliation={St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, St. Petersburg, 191036, Russian Federation},\\r\\nabstract={The diagnostics of tuberculosis infection, including immunological methods, evolved significant changes over time. Introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to differentiate between these conditions, thus requiring development and implementation of new diagnostic approaches. A prospective study enrolling two groups of patients was conducted as follows: group I (n = 50) included patients with verified pulmonary tuberculosis, MBT (+), whereas group II (n = 15) consisted of subjects with LTI, and control group was represented by healthy subjects (n = 14). The entire examination protocol included clinical, radiological, bacteriological, and immunological methods (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskin test). Immune complexes were determined in all patients and healthy individuals by means of dynamic light scattering after the in vitro addition of specific antigens (ESAT-6 and SFP-10 peptides). The data obtained have shown low informativity of clinical methods in diagnostics of pulmonary tuberculosis. In the presence of characteristic X-radiographic changes, bacteriological verification of tuberculosis was proven only in 46% of cases. Usage of various immunological tests allowed to obtain positive results in 84-90% of cases, along with 100% positivity in subjects with LTI. Detection of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases, and to identify the high-risk group for development of active tuberculosis among the subjects with latent tuberculosis infection. Conclusions: the obtained data may be applied both for diagnosis of active tuberculosis in the absence of verified diagnosis, but also enable identification of a high-risk group for development of the disease in subjects with latent tuberculosis infection. © 2019, SPb RAACI.},\\r\\nauthor_keywords={Dynamic light scattering method;  Immunological tests;  Latent tuberculosis infection;  Test with allergen tuberculosis recombinant;  Tuberculosis},\\r\\ncorrespondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},\\r\\npublisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},\\r\\nissn={15630625},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Med. Immunol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Starshinova, A.\",\"Istomina, E.\",\"Zinchenko, Y.\",\"Filatov, M.\",\"Landa, S.\",\"Burdakov, V.\",\"Belyaeva, E.\",\"Nazarenko, M.\",\"Sapozhnikova, N.\",\"Yablonkiy, P.\"],\"key\":\"Starshinova2019269\",\"id\":\"Starshinova2019269\",\"bibbaseid\":\"starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&doi=10.15789%2f1563-0625-2019-2-269-278&partnerID=40&md5=a90c16d4007b2884eb6918641062312c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Starshinova\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zinchenko\"],\"firstnames\":[\"Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Denisova\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Istomina\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Churilov\"],\"firstnames\":[\"L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sapozhnikova\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pavlova\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stepanenko\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mayevskaya\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yablonskiy\"],\"firstnames\":[\"P.\"],\"suffixes\":[]}],\"title\":\"Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis\",\"journal\":\"Immunologic Research\",\"year\":\"2018\",\"volume\":\"66\",\"number\":\"6\",\"pages\":\"737-743\",\"doi\":\"10.1007/s12026-018-9052-1\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&doi=10.1007%2fs12026-018-9052-1&partnerID=40&md5=5924fbaf3fd914e3c844ebfe2c48893c\",\"affiliation\":\"FGBU “St. Petersburg Scientific Research Institute of Phthisiopulmonology” of the Ministry of Health of Russia, St. Petersburg, Russian Federation; The St. Petersburg State University, St. Petersburg, Russian Federation; St. Petersburg Nuclear Physics Institute, NRC “Konstantinov Institute”, Gatchina, 188300, Russian Federation; SPb GBUZ “City hospital №2”, St. Petersburg, 195294, Russian Federation; The Saint Petersburg Federal State University of Economics, St. Petersburg, Russian Federation\",\"abstract\":\"Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data on the etiology of the disease. The exclusion of tuberculosis in every patient with suspected sarcoidosis is a mandatory stage of diagnosis, especially in countries with a high burden of tuberculosis. A prospective study was conducted with two groups of patients: group I (n = 50)—patients with pulmonary sarcoidosis established according to standard criteria; group II (n = 28)—patients with pulmonary tuberculosis with bacterial excretion. The control group (n = 24) was presented by healthy subjects. The examination complex included x-ray, bacteriological, immunological (Mantoux test with 2 TE, TB.SPOT test), and histological methods. All patients and healthy subjects were assessed for immune complexes with the use of the dynamic light scattering (DLS) method and adding of “healthy lung tissue extract” antigens and specific tuberculosis antigens ESAT-6 and SFP-10 in vitro. Significant differences were found in determining specific immune complexes in patients with pulmonary sarcoidosis and pulmonary tuberculosis. Registration of specific immune complex formation with “healthy lung tissue extract” in 100% cases may indicate the autoimmune nature of sarcoidosis. The absence of the immune complex formation in response to ESAT-6/SFP-10 antigens can be used for the differential diagnosis of two diseases. The diagnostic significance of the DLS method was 100% for sarcoidosis and 92.2% for tuberculosis. The data obtained in the study allows not only understanding the etiology of sarcoidosis, but also obtaining new criteria for the differential diagnosis of tuberculosis and pulmonary sarcoidosis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.\",\"author_keywords\":\"Allergen test with tuberculosis recombinant; Autoimmune diseases; Dynamic light scattering method; Immunological tests; Sarcoidosis; Tuberculosis\",\"funding_details\":\"Government Council on Grants, Russian Federation14.\",\"key\":\"Starshinova2018737\",\"id\":\"Starshinova2018737\",\"bibbaseid\":\"anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&doi=10.1007%2fs12026-018-9052-1&partnerID=40&md5=5924fbaf3fd914e3c844ebfe2c48893c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Petrenko\"],\"firstnames\":[\"E.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kopylov\"],\"firstnames\":[\"A.T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kleist\"],\"firstnames\":[\"O.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Legina\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]}],\"title\":\"Searching for Specific Markers of Glioblastoma: Analysis of Glioblastoma Cell Proteoforms\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2018\",\"volume\":\"12\",\"number\":\"6\",\"pages\":\"455-459\",\"doi\":\"10.1134/S1990519X18060068\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&doi=10.1134%2fS1990519X18060068&partnerID=40&md5=1a3891a94ef69f0b40bda4c79c6c8dfe\",\"affiliation\":\"Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russian Federation; Konstantinov Petersburg Nuclear Physics Institute of the National Research Center Kurchatov Institute, Gatchina, 188300, Russian Federation\",\"abstract\":\"Abstract: Using the sectional analysis of two-dimensional electrophoretic gels with liquid chromatography-mass spectrometry, proteoform profiles for individual genes expressed in cancer (glioblastoma) and normal (FLEH) cells were obtained. Profiles of more than 5000 genes were analyzed. It turned out that many genes encoding potential biomarkers of glioblastoma are characterized by sets of proteoforms that are different in normal and cancer cells. These proteoforms could be sources of highly specific markers and targets for therapy. Using a section analysis of two-dimensional electrophoretic gels with liquid chromatography by mass spectrometry, proteoform profiles were obtained for individual genes expressed in cancer (glioblastoma) and normal (FLEH) cells. Profiles of more than 5000 genes were analyzed. It turned out that many genes encoding potential biomarkers of glioblastoma are characterized by sets of proteoforms, which are different in normal and cancer cells. These proteoforms could be sources of highly specific markers and targets for therapy. © 2018, Pleiades Publishing, Ltd.\",\"author_keywords\":\"biomarker; glioblastoma; mass spectrometry; proteoform; proteome; two-dimensional electrophoresis\",\"funding_details\":\"Ministry of Education and Science of the Russian FederationRFMEFI62117X0017, 14.621.21.0017\",\"key\":\"Petrenko2018455\",\"id\":\"Petrenko2018455\",\"bibbaseid\":\"anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&doi=10.1134%2fS1990519X18060068&partnerID=40&md5=1a3891a94ef69f0b40bda4c79c6c8dfe\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Chernous\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shagimuratov\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ievenko\"],\"firstnames\":[\"I.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Efishov\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shvets\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kalitenkov\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]}],\"title\":\"Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals\",\"journal\":\"Russian Journal of Physical Chemistry B\",\"year\":\"2018\",\"volume\":\"12\",\"number\":\"3\",\"pages\":\"562-567\",\"doi\":\"10.1134/S1990793118030065\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&doi=10.1134%2fS1990793118030065&partnerID=40&md5=bef4391cf3e6455e8b4c238f9b0e484a\",\"affiliation\":\"Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Kaliningrad Branch, Russian Academy of Sciences, Kaliningrad, 236017, Russian Federation; Institute for Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677098, Russian Federation; Murmansk State Technical University, Sportivnaya 13, Murmansk, 183010, Russian Federation; Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russian Federation\",\"abstract\":\"A comparative analysis of total electron content (TEC) fluctuations and auroral activity, which characterizes the polar ionosphere during periods of substorm activity, is performed. The analysis is based on GPS/GLONASS observations at auroral and subauroral stations and data on auroral emissions obtained at the Yakutsk subauroral station and the Poker Flat (Alaska) auroral station. A detailed analysis is carried out for the storm on January 7, 2015. During this event, a sharp increase in the auroral activity and in TEC fluctuations from 09 to 13 UT was observed. A similarity of the dynamics of the auroral oval and the space–time distribution of TEC fluctuations associated with ionospheric irregularities in the oval is demonstrated. There is a close correlation between the positions of the auroral oval and the irregularity oval. The positions of the auroral oval, predicted by the NORUSKA Russian–Norwegian model, and of the TEC irregularity oval are compared. In general, the positions of the ovals are close to each other. The existing discrepancy may be due to the fact that the auroral oval is projected to an altitude of 110 km, whereas the irregularity oval, to an altitude of 450 km, and that the curvature of the magnetic force lines is not taken into account. The influence of auroral disturbances on navigational measurements manifests itself through the fact that, during disturbances, faults and failures in the operation of navigation equipment at auroral and subauroral stations increase. © 2018, Pleiades Publishing, Ltd.\",\"author_keywords\":\"auroral oval; auroral perturbations; GPS/GLONASS; TEC fluctuations; TEC irregularity oval\",\"correspondence_address1\":\"Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of SciencesRussian Federation; email: chernouss@pgia.ru\",\"publisher\":\"Pleiades Publishing\",\"issn\":\"19907931\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Phys. Chem. B\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Chernous2018562,\\r\\nauthor={Chernous, S.A. and Shagimuratov, I.I. and Ievenko, I.B. and Filatov, M.V. and Efishov, I.I. and Shvets, M.V. and Kalitenkov, N.V.},\\r\\ntitle={Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals},\\r\\njournal={Russian Journal of Physical Chemistry B},\\r\\nyear={2018},\\r\\nvolume={12},\\r\\nnumber={3},\\r\\npages={562-567},\\r\\ndoi={10.1134/S1990793118030065},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&doi=10.1134%2fS1990793118030065&partnerID=40&md5=bef4391cf3e6455e8b4c238f9b0e484a},\\r\\naffiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Kaliningrad Branch, Russian Academy of Sciences, Kaliningrad, 236017, Russian Federation; Institute for Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677098, Russian Federation; Murmansk State Technical University, Sportivnaya 13, Murmansk, 183010, Russian Federation; Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russian Federation},\\r\\nabstract={A comparative analysis of total electron content (TEC) fluctuations and auroral activity, which characterizes the polar ionosphere during periods of substorm activity, is performed. The analysis is based on GPS/GLONASS observations at auroral and subauroral stations and data on auroral emissions obtained at the Yakutsk subauroral station and the Poker Flat (Alaska) auroral station. A detailed analysis is carried out for the storm on January 7, 2015. During this event, a sharp increase in the auroral activity and in TEC fluctuations from 09 to 13 UT was observed. A similarity of the dynamics of the auroral oval and the space–time distribution of TEC fluctuations associated with ionospheric irregularities in the oval is demonstrated. There is a close correlation between the positions of the auroral oval and the irregularity oval. The positions of the auroral oval, predicted by the NORUSKA Russian–Norwegian model, and of the TEC irregularity oval are compared. In general, the positions of the ovals are close to each other. The existing discrepancy may be due to the fact that the auroral oval is projected to an altitude of 110 km, whereas the irregularity oval, to an altitude of 450 km, and that the curvature of the magnetic force lines is not taken into account. The influence of auroral disturbances on navigational measurements manifests itself through the fact that, during disturbances, faults and failures in the operation of navigation equipment at auroral and subauroral stations increase. © 2018, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={auroral oval;  auroral perturbations;  GPS/GLONASS;  TEC fluctuations;  TEC irregularity oval},\\r\\ncorrespondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of SciencesRussian Federation; email: chernouss@pgia.ru},\\r\\npublisher={Pleiades Publishing},\\r\\nissn={19907931},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Phys. Chem. B},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Chernous, S.\",\"Shagimuratov, I.\",\"Ievenko, I.\",\"Filatov, M.\",\"Efishov, I.\",\"Shvets, M.\",\"Kalitenkov, N.\"],\"key\":\"Chernous2018562\",\"id\":\"Chernous2018562\",\"bibbaseid\":\"chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&doi=10.1134%2fS1990793118030065&partnerID=40&md5=bef4391cf3e6455e8b4c238f9b0e484a\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makarov\"],\"firstnames\":[\"E.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2018\",\"volume\":\"12\",\"number\":\"1\",\"pages\":\"20-26\",\"doi\":\"10.1134/S1990519X18010030\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&doi=10.1134%2fS1990519X18010030&partnerID=40&md5=f9de8e8533842e75b6479cbae6afc729\",\"affiliation\":\"Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; College of Health and Life Sciences, Brunel University, London, Uxbridge, United Kingdom; St. Petersburg Institute of Phthisiopulmonology, St. Petersburg, 191036, Russian Federation\",\"abstract\":\"Exosomes are nanosized vesicles that are secreted by many types of cells. We have found that exosomes secreted by HEK293 and HT-1080 can suppress growth and proliferation of p53-deficient cells. Upon overexpression of exogenous p53-GFP in HEK293 cells, we observed p53 protein in exosomes that were secreted by these cells. We also found endogenous p53 in exosomes that were secreted by HT-1080 cells with a higher level of p53 expression. We were able to detect endogenous p53 protein in exosomes that originated from human plasma and were transferred to p53-deficient cells. Our findings indicate that p53 protein can be transferred between cells and may play an important physiological role. © 2018, Pleiades Publishing, Ltd.\",\"author_keywords\":\"cell lines; exosomes; р53\",\"correspondence_address1\":\"Filatov, M.V.; Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”Russian Federation; email: fil_53@mail.ru\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Burdakov201820,\\r\\nauthor={Burdakov, V.S. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Filatov, M.V.},\\r\\ntitle={Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2018},\\r\\nvolume={12},\\r\\nnumber={1},\\r\\npages={20-26},\\r\\ndoi={10.1134/S1990519X18010030},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&doi=10.1134%2fS1990519X18010030&partnerID=40&md5=f9de8e8533842e75b6479cbae6afc729},\\r\\naffiliation={Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; College of Health and Life Sciences, Brunel University, London, Uxbridge, United Kingdom; St. Petersburg Institute of Phthisiopulmonology, St. Petersburg, 191036, Russian Federation},\\r\\nabstract={Exosomes are nanosized vesicles that are secreted by many types of cells. We have found that exosomes secreted by HEK293 and HT-1080 can suppress growth and proliferation of p53-deficient cells. Upon overexpression of exogenous p53-GFP in HEK293 cells, we observed p53 protein in exosomes that were secreted by these cells. We also found endogenous p53 in exosomes that were secreted by HT-1080 cells with a higher level of p53 expression. We were able to detect endogenous p53 protein in exosomes that originated from human plasma and were transferred to p53-deficient cells. Our findings indicate that p53 protein can be transferred between cells and may play an important physiological role. © 2018, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={cell lines;  exosomes;  р53},\\r\\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”Russian Federation; email: fil_53@mail.ru},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Burdakov, V.\",\"Kovalev, R.\",\"Pantina, R.\",\"Varfolomeeva, E.\",\"Makarov, E.\",\"Filatov, M.\"],\"key\":\"Burdakov201820\",\"id\":\"Burdakov201820\",\"bibbaseid\":\"burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&doi=10.1134%2fS1990519X18010030&partnerID=40&md5=f9de8e8533842e75b6479cbae6afc729\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]}],\"title\":\"Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same\",\"journal\":\"Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry\",\"year\":\"2018\",\"volume\":\"12\",\"number\":\"1\",\"pages\":\"39-42\",\"doi\":\"10.1134/S199075081801002X\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&doi=10.1134%2fS199075081801002X&partnerID=40&md5=5c507aecac55632ff5640f00b0f7139c\",\"affiliation\":\"Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov Institute, Leningrad region, Gatchina, 188300, Russian Federation\",\"abstract\":\"Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-toback principle. Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well. © 2018, Pleiades Publishing, Ltd.\",\"author_keywords\":\"cytoplasm; double trimer; monomer; neutrophil; PCNA; structure\",\"correspondence_address1\":\"Naryzhny, S.N.; Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov InstituteRussian Federation; email: snaryzhny@mail.ru\",\"publisher\":\"Pleiades Publishing\",\"issn\":\"19907508\",\"language\":\"English\",\"abbrev_source_title\":\"Biochem. (Moscow) Suppl. Ser. B Biomed. Chem.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Belyakova201839,\\r\\nauthor={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},\\r\\ntitle={Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same},\\r\\njournal={Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry},\\r\\nyear={2018},\\r\\nvolume={12},\\r\\nnumber={1},\\r\\npages={39-42},\\r\\ndoi={10.1134/S199075081801002X},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&doi=10.1134%2fS199075081801002X&partnerID=40&md5=5c507aecac55632ff5640f00b0f7139c},\\r\\naffiliation={Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov Institute, Leningrad region, Gatchina, 188300, Russian Federation},\\r\\nabstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-toback principle. Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well. © 2018, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={cytoplasm;  double trimer;  monomer;  neutrophil;  PCNA;  structure},\\r\\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov InstituteRussian Federation; email: snaryzhny@mail.ru},\\r\\npublisher={Pleiades Publishing},\\r\\nissn={19907508},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Biochem. (Moscow) Suppl. Ser. B Biomed. Chem.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Belyakova, N.\",\"Pantina, R.\",\"Kovalev, R.\",\"Filatov, M.\",\"Naryzhny, S.\"],\"key\":\"Belyakova201839\",\"id\":\"Belyakova201839\",\"bibbaseid\":\"belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&doi=10.1134%2fS199075081801002X&partnerID=40&md5=5c507aecac55632ff5640f00b0f7139c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Korabliov\"],\"firstnames\":[\"P.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations]\",\"journal\":\"F1000Research\",\"year\":\"2018\",\"volume\":\"7\",\"doi\":\"10.12688/f1000research.14406.1\",\"art_number\":\"618\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&doi=10.12688%2ff1000research.14406.1&partnerID=40&md5=5cdb17d6a9583e9bc8443ee0ec1012f7\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, National Research Center, Gatchina, 188300, Russian Federation; State Research Institute Center for Innovative Medicine, Vilnius, LT-01102, Lithuania; Saint Petersburg State Res Inst of Phthisiopulmonology of the Min of Healthcare of the Russian Fed, Saint Petersburg, 191036, Russian Federation\",\"abstract\":\"Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body's immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7-8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular-cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely. © 2018 Landa SB et al.\",\"author_keywords\":\"Digestion; Dynamic light scattering method; irculating immune complexes; Isotypes of immunoglobulins\",\"correspondence_address1\":\"Filatov, M.V.; Division of Molecular and Radiation Biophysics, National Research CenterRussian Federation; email: fil_53@mail.ru\",\"publisher\":\"F1000 Research Ltd\",\"issn\":\"20461402\",\"language\":\"English\",\"abbrev_source_title\":\"F1000 Res.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov2018,\\r\\nauthor={Filatov, M.V. and Landa, S.B. and Korabliov, P.V. and Semenova, E.V.},\\r\\ntitle={Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations]},\\r\\njournal={F1000Research},\\r\\nyear={2018},\\r\\nvolume={7},\\r\\ndoi={10.12688/f1000research.14406.1},\\r\\nart_number={618},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&doi=10.12688%2ff1000research.14406.1&partnerID=40&md5=5cdb17d6a9583e9bc8443ee0ec1012f7},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, National Research Center, Gatchina, 188300, Russian Federation; State Research Institute Center for Innovative Medicine, Vilnius, LT-01102, Lithuania; Saint Petersburg State Res Inst of Phthisiopulmonology of the Min of Healthcare of the Russian Fed, Saint Petersburg, 191036, Russian Federation},\\r\\nabstract={Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body's immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7-8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular-cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely. © 2018 Landa SB et al.},\\r\\nauthor_keywords={Digestion;  Dynamic light scattering method;  irculating immune complexes;  Isotypes of immunoglobulins},\\r\\ncorrespondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, National Research CenterRussian Federation; email: fil_53@mail.ru},\\r\\npublisher={F1000 Research Ltd},\\r\\nissn={20461402},\\r\\nlanguage={English},\\r\\nabbrev_source_title={F1000 Res.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Landa, S.\",\"Korabliov, P.\",\"Semenova, E.\"],\"key\":\"Filatov2018\",\"id\":\"Filatov2018\",\"bibbaseid\":\"filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&doi=10.12688%2ff1000research.14406.1&partnerID=40&md5=5cdb17d6a9583e9bc8443ee0ec1012f7\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sokolov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kostevich\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grigorieva\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gorudko\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kozlov\"],\"firstnames\":[\"S.O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kudryavtsev\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mikhalchik\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Cherenkevich\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Panasenko\"],\"firstnames\":[\"O.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Arnhold\"],\"firstnames\":[\"J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vasilyev\"],\"firstnames\":[\"V.B.\"],\"suffixes\":[]}],\"title\":\"Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase\",\"journal\":\"Biochemistry and Cell Biology\",\"year\":\"2018\",\"volume\":\"96\",\"number\":\"4\",\"pages\":\"457-467\",\"doi\":\"10.1139/bcb-2017-0277\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&doi=10.1139%2fbcb-2017-0277&partnerID=40&md5=78f514263ecc17ebbca9dc2f1d6906ac\",\"affiliation\":\"FSBSI Institute of Experimental Medicine, Saint-Petersburg, 197376, Russian Federation; Federal Research and Clinical Center of Physical, Chemical Medicine of Federal Medical Biological Agency, Moscow, 119435, Russian Federation; Saint-Petersburg State University, Saint-Petersburg, 199034, Russian Federation; Centre of Preclinical Translational Research, Almazov National Medical Research Centre, Saint-Petersburg, 197371, Russian Federation; National Research Centre Kurchatov Institute, B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Department of Biophysics, Belarusian State University, Minsk, 220030, Belarus; Far Eastern Federal University, Vladivostok, 690090, Russian Federation; Pirogov Russian National Research Medical University, Moscow, 117997, Russian Federation; Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, 04107, Germany\",\"abstract\":\"CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications. © 2018 Published by NRC Research Press.\",\"author_keywords\":\"Ceruloplasmin; Myeloperoxidase; Neutrophils; Respiratory burst; Superoxide dismutase\",\"funding_details\":\"17-75-30064\",\"key\":\"Sokolov2018457\",\"id\":\"Sokolov2018457\",\"bibbaseid\":\"anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&doi=10.1139%2fbcb-2017-0277&partnerID=40&md5=78f514263ecc17ebbca9dc2f1d6906ac\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Petrenko\"],\"firstnames\":[\"E.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kopylov\"],\"firstnames\":[\"A.T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kleist\"],\"firstnames\":[\"O.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Legina\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]}],\"title\":\"In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells\",\"journal\":\"Tsitologiya\",\"year\":\"2018\",\"volume\":\"60\",\"number\":\"7\",\"pages\":\"519-523\",\"doi\":\"10.31116/tsitol.2018.07.05\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&doi=10.31116%2ftsitol.2018.07.05&partnerID=40&md5=4fb79a48075054ea3847204606cafb5e\",\"affiliation\":\"V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russian Federation; B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre Kurchatov Institute, Gatchina, Leningrad Region, 188300, Russian Federation\",\"abstract\":\"Proteins from normal (fibroblasts) and cancer (glioblastoma) human cells were separated by two dimensional gel electrophoresis. Next, a sectional analysis of these gels by mass spectrometry was performed, and profiles of proteoforms coded by more than 5000 genes were obtained. It was found that some proteins have specific for glioblastoma cells proteoforms. We assume that these proteoforms could be used as specific for glioblastoma biomarkers. © 2018 Sankt Peterburg. All rights reserved.\",\"author_keywords\":\"Biomarker; Glioblastoma; Mass spectrometry; Proteoforma; Proteome; Two dimensional electrophoresis\",\"correspondence_address1\":\"Naryzhny, S.N.; V. N. Orekhovich Research Institute of Biomedical ChemistryRussian Federation; email: naryzhnyy_sn@pnpi.nrcki.ru\",\"publisher\":\"Sankt Peterburg\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Petrenko2018519,\\r\\nauthor={Petrenko, E.S. and Kopylov, A.T. and Kleist, O.A. and Legina, O.K. and Belyakova, N.V. and Pantina, R.A. and Naryzhny, S.N.},\\r\\ntitle={In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells},\\r\\njournal={Tsitologiya},\\r\\nyear={2018},\\r\\nvolume={60},\\r\\nnumber={7},\\r\\npages={519-523},\\r\\ndoi={10.31116/tsitol.2018.07.05},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&doi=10.31116%2ftsitol.2018.07.05&partnerID=40&md5=4fb79a48075054ea3847204606cafb5e},\\r\\naffiliation={V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russian Federation; B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre Kurchatov Institute, Gatchina, Leningrad Region, 188300, Russian Federation},\\r\\nabstract={Proteins from normal (fibroblasts) and cancer (glioblastoma) human cells were separated by two dimensional gel electrophoresis. Next, a sectional analysis of these gels by mass spectrometry was performed, and profiles of proteoforms coded by more than 5000 genes were obtained. It was found that some proteins have specific for glioblastoma cells proteoforms. We assume that these proteoforms could be used as specific for glioblastoma biomarkers. © 2018 Sankt Peterburg. All rights reserved.},\\r\\nauthor_keywords={Biomarker;  Glioblastoma;  Mass spectrometry;  Proteoforma;  Proteome;  Two dimensional electrophoresis},\\r\\ncorrespondence_address1={Naryzhny, S.N.; V. N. Orekhovich Research Institute of Biomedical ChemistryRussian Federation; email: naryzhnyy_sn@pnpi.nrcki.ru},\\r\\npublisher={Sankt Peterburg},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Petrenko, E.\",\"Kopylov, A.\",\"Kleist, O.\",\"Legina, O.\",\"Belyakova, N.\",\"Pantina, R.\",\"Naryzhny, S.\"],\"key\":\"Petrenko2018519\",\"id\":\"Petrenko2018519\",\"bibbaseid\":\"petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&doi=10.31116%2ftsitol.2018.07.05&partnerID=40&md5=4fb79a48075054ea3847204606cafb5e\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kiseleva\"],\"firstnames\":[\"L.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kartashev\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vartanyan\"],\"firstnames\":[\"N.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pinevich\"],\"firstnames\":[\"A.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Samoilovich\"],\"firstnames\":[\"M.P.\"],\"suffixes\":[]}],\"title\":\"Characterization of New Human Glioblastoma Cell Lines\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2018\",\"volume\":\"12\",\"number\":\"1\",\"doi\":\"10.1134/S1990519X18010108\",\"note\":\"cited By 5\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&doi=10.1134%2fS1990519X18010108&partnerID=40&md5=c3954c3ded80c915486fe0c5d05ab388\",\"affiliation\":\"Russian Research Center for Radiology and Surgical Technologies, St. Petersburg, 197758, Russian Federation; St. Petersburg State University, St. Petersburg, 199034, Russian Federation; Northwestern State Medical University named after I.I. Mechnikov, St. Petersburg, 191015, Russian Federation; St. Petersburg Nuclear Physics Institute named after B.P. Konstantinov, St. Petersburg, 188300, Russian Federation\",\"abstract\":\"Glioblastomas are tumors of neuroectodermal origin with a high degree of diversity. Tumor cells isolated from the surgical material of patients with glioblastomas are heterogeneous populations varying in morphology, phenotype, and genetic characteristics. This paper presents a description of two new glioblastoma cell lines, R1 and T2, isolated from tumor tissue of patients in 2010. We investigated their morphological and cytochemical cell characteristics and the expression of neuronal, mesenchymal, and endothelial markers, as well as the activity of genes encoding a number of growth factors, extracellular matrix proteins, and intracellular proteins typical for cells of mesenchymal origin. R1 and T2 cell lines are morphologically different. T2 cell line was characterized by the presence of multinuclear cells. In terms of the expression of β-tubulin III, MGMT, and p53 protein, R1 cell line was more heterogeneous than T2. R1 and T2 glioblastoma cell lines also differed in the presence and ratio of cell populations with mesenchymal, neuronal, and endothelial markers. Thus, neuronal markers CD133/2 and CD56 were detected only on R1 cells. Both lines were characterized by high activity of growth factor genes TGFβ1, VEGF, and FGF2(b), lower activity of EGF, and high expression of THBS1 and αSMA genes. However, the activity of most of the genes under study in R1 cells was higher than in T2 cells. The greatest difference lay in the expression of HGF, FAP, and TNC. Comparison of two new glioblastoma cell lines, R1 and T2, with the continuously cultivated lines А172 and T98G showed that R1 line had remarkable similarity with A172, while T2 glioblastoma resembled T98G cells. Apparently, the differences between R1 and T2 cell lines are determined by the properties of the initial tumors rather than the time of the cell cultivation. © 2018, Pleiades Publishing, Ltd.\",\"author_keywords\":\"A172; gene expression; glioblastoma cell lines; growth factors; surface markers; T98G\",\"correspondence_address1\":\"Kiseleva, L.N.; Russian Research Center for Radiology and Surgical TechnologiesRussian Federation; email: luba_kiseleva@mail.ru\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kiseleva2018,\\r\\nauthor={Kiseleva, L.N. and Kartashev, A.V. and Vartanyan, N.L. and Pinevich, A.A. and Filatov, M.V. and Samoilovich, M.P.},\\r\\ntitle={Characterization of New Human Glioblastoma Cell Lines},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2018},\\r\\nvolume={12},\\r\\nnumber={1},\\r\\ndoi={10.1134/S1990519X18010108},\\r\\nnote={cited By 5},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&doi=10.1134%2fS1990519X18010108&partnerID=40&md5=c3954c3ded80c915486fe0c5d05ab388},\\r\\naffiliation={Russian Research Center for Radiology and Surgical Technologies, St. Petersburg, 197758, Russian Federation; St. Petersburg State University, St. Petersburg, 199034, Russian Federation; Northwestern State Medical University named after I.I. Mechnikov, St. Petersburg, 191015, Russian Federation; St. Petersburg Nuclear Physics Institute named after B.P. Konstantinov, St. Petersburg, 188300, Russian Federation},\\r\\nabstract={Glioblastomas are tumors of neuroectodermal origin with a high degree of diversity. Tumor cells isolated from the surgical material of patients with glioblastomas are heterogeneous populations varying in morphology, phenotype, and genetic characteristics. This paper presents a description of two new glioblastoma cell lines, R1 and T2, isolated from tumor tissue of patients in 2010. We investigated their morphological and cytochemical cell characteristics and the expression of neuronal, mesenchymal, and endothelial markers, as well as the activity of genes encoding a number of growth factors, extracellular matrix proteins, and intracellular proteins typical for cells of mesenchymal origin. R1 and T2 cell lines are morphologically different. T2 cell line was characterized by the presence of multinuclear cells. In terms of the expression of β-tubulin III, MGMT, and p53 protein, R1 cell line was more heterogeneous than T2. R1 and T2 glioblastoma cell lines also differed in the presence and ratio of cell populations with mesenchymal, neuronal, and endothelial markers. Thus, neuronal markers CD133/2 and CD56 were detected only on R1 cells. Both lines were characterized by high activity of growth factor genes TGFβ1, VEGF, and FGF2(b), lower activity of EGF, and high expression of THBS1 and αSMA genes. However, the activity of most of the genes under study in R1 cells was higher than in T2 cells. The greatest difference lay in the expression of HGF, FAP, and TNC. Comparison of two new glioblastoma cell lines, R1 and T2, with the continuously cultivated lines А172 and T98G showed that R1 line had remarkable similarity with A172, while T2 glioblastoma resembled T98G cells. Apparently, the differences between R1 and T2 cell lines are determined by the properties of the initial tumors rather than the time of the cell cultivation. © 2018, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={A172;  gene expression;  glioblastoma cell lines;  growth factors;  surface markers;  T98G},\\r\\ncorrespondence_address1={Kiseleva, L.N.; Russian Research Center for Radiology and Surgical TechnologiesRussian Federation; email: luba_kiseleva@mail.ru},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kiseleva, L.\",\"Kartashev, A.\",\"Vartanyan, N.\",\"Pinevich, A.\",\"Filatov, M.\",\"Samoilovich, M.\"],\"key\":\"Kiseleva2018\",\"id\":\"Kiseleva2018\",\"bibbaseid\":\"kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&doi=10.1134%2fS1990519X18010108&partnerID=40&md5=c3954c3ded80c915486fe0c5d05ab388\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Samsonov\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kamyshinsky\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Verlov\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vasiliev\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malek\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]}],\"title\":\"Exosomes: Some approaches to cancer diagnosis and therapy\",\"journal\":\"AIP Conference Proceedings\",\"year\":\"2017\",\"volume\":\"1882\",\"doi\":\"10.1063/1.5001645\",\"art_number\":\"020066\",\"note\":\"cited By 5; Conference of International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference Date: 23 May 2017 Through 26 May 2017; Conference Code:133914\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&doi=10.1063%2f1.5001645&partnerID=40&md5=d908b66243d4ade3a27c45bad492f886\",\"affiliation\":\"Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov Institute, Gatchina, Russian Federation; Petrov Institute of Oncology, Saint-Petersburg, Russian Federation; Ltd. Oncosystem, Skolkovo, Russian Federation; Peter the Great Saint-Petersburg Polytechnic University, St. Petersburg, Russian Federation; National Research Center, Kurchatov Institute, Moscow, Russian Federation\",\"abstract\":\"Exosomes are membrane-bound, intercellular communication shuttle vesicles that are defined by their endocytic origin and size range of 30-120 nm. Secreted by nearly all mammalian cell types and present in bodily fluids, exosomes confer messages between cells, by transporting functionally relevant proteins, nucleic acids, and lipids. The capability of tumor exosomes to house tumorigenic information and induce cellular responses that promote disease pathogenesis make tumor exosomes an attractive tool in identifying cancer biomarkers and exploiting exosomes for therapy. In this paper, we sum up our previous findings to utilize exosomes as biomarkers for early detection, diagnosis and therapy selection of prostate and thyroid cancer and present our results on exosomes in colon cancer. Some of plasma exosomal miRNAs showed their potential as diagnostic markers for colon cancer. All together, the data suggested the potentials of circulating exosomal miRNAs as liquid biopsy markers for cancer. Here we also present the possibilities of delivering therapeutic molecules by exosomes. Previously, we had demonstrated the potential of exosome-mediated siRNA delivery. Here, we present the possibility of carrying the exogenous p53 protein by exosomes in vitro. © 2017 Author(s).\",\"correspondence_address1\":\"Shtam, T.; Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov InstituteRussian Federation; email: tatyana_shtam@mail.ru\",\"editor\":[{\"propositions\":[],\"lastnames\":[\"Gutmanas\",\"E.Y.\"],\"firstnames\":[\"Naimark\",\"O.B.\"],\"suffixes\":[]}],\"sponsors\":\"Institute of Continuous Media Mechanics of UrB RAS; Institute of Strength Physics and Materials Science of SB RAS; National Research Tomsk Polytechnic University; National Research Tomsk State University; Tomsk Research Institute of Oncology\",\"publisher\":\"American Institute of Physics Inc.\",\"issn\":\"0094243X\",\"isbn\":\"9780735415621\",\"language\":\"English\",\"abbrev_source_title\":\"AIP Conf. Proc.\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Shtam2017,\\r\\nauthor={Shtam, T. and Samsonov, R. and Kamyshinsky, R. and Pantina, R. and Verlov, N. and Vasiliev, A. and Konevega, A.L. and Malek, A.V.},\\r\\ntitle={Exosomes: Some approaches to cancer diagnosis and therapy},\\r\\njournal={AIP Conference Proceedings},\\r\\nyear={2017},\\r\\nvolume={1882},\\r\\ndoi={10.1063/1.5001645},\\r\\nart_number={020066},\\r\\nnote={cited By 5; Conference of International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference Date: 23 May 2017 Through 26 May 2017;  Conference Code:133914},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&doi=10.1063%2f1.5001645&partnerID=40&md5=d908b66243d4ade3a27c45bad492f886},\\r\\naffiliation={Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov Institute, Gatchina, Russian Federation; Petrov Institute of Oncology, Saint-Petersburg, Russian Federation; Ltd. Oncosystem, Skolkovo, Russian Federation; Peter the Great Saint-Petersburg Polytechnic University, St. Petersburg, Russian Federation; National Research Center, Kurchatov Institute, Moscow, Russian Federation},\\r\\nabstract={Exosomes are membrane-bound, intercellular communication shuttle vesicles that are defined by their endocytic origin and size range of 30-120 nm. Secreted by nearly all mammalian cell types and present in bodily fluids, exosomes confer messages between cells, by transporting functionally relevant proteins, nucleic acids, and lipids. The capability of tumor exosomes to house tumorigenic information and induce cellular responses that promote disease pathogenesis make tumor exosomes an attractive tool in identifying cancer biomarkers and exploiting exosomes for therapy. In this paper, we sum up our previous findings to utilize exosomes as biomarkers for early detection, diagnosis and therapy selection of prostate and thyroid cancer and present our results on exosomes in colon cancer. Some of plasma exosomal miRNAs showed their potential as diagnostic markers for colon cancer. All together, the data suggested the potentials of circulating exosomal miRNAs as liquid biopsy markers for cancer. Here we also present the possibilities of delivering therapeutic molecules by exosomes. Previously, we had demonstrated the potential of exosome-mediated siRNA delivery. Here, we present the possibility of carrying the exogenous p53 protein by exosomes in vitro. © 2017 Author(s).},\\r\\ncorrespondence_address1={Shtam, T.; Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov InstituteRussian Federation; email: tatyana_shtam@mail.ru},\\r\\neditor={Gutmanas E.Y., Naimark O.B., Sharkeev Y.P.},\\r\\nsponsors={Institute of Continuous Media Mechanics of UrB RAS; Institute of Strength Physics and Materials Science of SB RAS; National Research Tomsk Polytechnic University; National Research Tomsk State University; Tomsk Research Institute of Oncology},\\r\\npublisher={American Institute of Physics Inc.},\\r\\nissn={0094243X},\\r\\nisbn={9780735415621},\\r\\nlanguage={English},\\r\\nabbrev_source_title={AIP Conf. Proc.},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Samsonov, R.\",\"Kamyshinsky, R.\",\"Pantina, R.\",\"Verlov, N.\",\"Vasiliev, A.\",\"Konevega, A.\",\"Malek, A.\"],\"editor_short\":[\"Gutmanas E.Y., N. O.\"],\"key\":\"Shtam2017\",\"id\":\"Shtam2017\",\"bibbaseid\":\"shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&doi=10.1063%2f1.5001645&partnerID=40&md5=d908b66243d4ade3a27c45bad492f886\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":1,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Makarov\"],\"firstnames\":[\"E.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis\",\"journal\":\"PLoS ONE\",\"year\":\"2017\",\"volume\":\"12\",\"number\":\"9\",\"doi\":\"10.1371/journal.pone.0185126\",\"art_number\":\"e0185126\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&doi=10.1371%2fjournal.pone.0185126&partnerID=40&md5=e33272f5dbd1aae05eb33bbe72e9eea2\",\"affiliation\":\"Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; Peter the Great St Petersburg Polytechnic University, St Petersburg, Russian Federation; Petrov Institute of Oncology, St Petersburg, Russian Federation\",\"abstract\":\"P53 protein is more frequently mutated in human tumours compared with the other proteins. While the majority of the p53 mutations, especially within its DNA-binding domain, lead to the loss of the wild-type function, there are accumulating data demonstrating that the p53 mutants gain tumour promoting activities; the latter triggers a revitalised interest in functional analysis of the p53 mutants. A systematic screening for p53 mutations in surgical materials from patients with glioma revealed a 378C>G mutation that creates a stop codon at the position of amino acid residue 126. The mutation eliminates the recognition site for the restriction endonuclease Sca I that allowed us to carry out RFLP analysis of DNA extracted from the clinical samples and suggests that this mutation is more frequent than is documented in the p53 databases. Both the ECV-304 and EJ cell lines, that probably originate from the bladder carcinoma T24 cell line, were confirmed to contain the homozygous 378C>G mutation but were shown to produce the p53 protein of expected full-length size detected by Western blotting. We provide evidence that the 378C>G mutation generates an alternative 3’ splice site (ss) which is more often used instead of the authentic upstream 3’ ss, driving the production of mRNA encoding the protein with the single amino acid deletion (p53ΔY126). Using endogenous expression, we demonstrated that the p53ΔY126 protein is nearly as active as the wild type protein in inducing the p21/Waf1 expression and apoptosis. © 2017 Makarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.\",\"correspondence_address1\":\"Filatov, M.V.; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil53ster@gmail.com\",\"publisher\":\"Public Library of Science\",\"issn\":\"19326203\",\"coden\":\"POLNC\",\"pubmed_id\":\"28961258\",\"language\":\"English\",\"abbrev_source_title\":\"PLoS ONE\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Makarov2017,\\r\\nauthor={Makarov, E.M. and Shtam, T.A. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Filatov, M.V.},\\r\\ntitle={The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis},\\r\\njournal={PLoS ONE},\\r\\nyear={2017},\\r\\nvolume={12},\\r\\nnumber={9},\\r\\ndoi={10.1371/journal.pone.0185126},\\r\\nart_number={e0185126},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&doi=10.1371%2fjournal.pone.0185126&partnerID=40&md5=e33272f5dbd1aae05eb33bbe72e9eea2},\\r\\naffiliation={Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; Peter the Great St Petersburg Polytechnic University, St Petersburg, Russian Federation; Petrov Institute of Oncology, St Petersburg, Russian Federation},\\r\\nabstract={P53 protein is more frequently mutated in human tumours compared with the other proteins. While the majority of the p53 mutations, especially within its DNA-binding domain, lead to the loss of the wild-type function, there are accumulating data demonstrating that the p53 mutants gain tumour promoting activities; the latter triggers a revitalised interest in functional analysis of the p53 mutants. A systematic screening for p53 mutations in surgical materials from patients with glioma revealed a 378C>G mutation that creates a stop codon at the position of amino acid residue 126. The mutation eliminates the recognition site for the restriction endonuclease Sca I that allowed us to carry out RFLP analysis of DNA extracted from the clinical samples and suggests that this mutation is more frequent than is documented in the p53 databases. Both the ECV-304 and EJ cell lines, that probably originate from the bladder carcinoma T24 cell line, were confirmed to contain the homozygous 378C>G mutation but were shown to produce the p53 protein of expected full-length size detected by Western blotting. We provide evidence that the 378C>G mutation generates an alternative 3’ splice site (ss) which is more often used instead of the authentic upstream 3’ ss, driving the production of mRNA encoding the protein with the single amino acid deletion (p53ΔY126). Using endogenous expression, we demonstrated that the p53ΔY126 protein is nearly as active as the wild type protein in inducing the p21/Waf1 expression and apoptosis. © 2017 Makarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},\\r\\ncorrespondence_address1={Filatov, M.V.; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil53ster@gmail.com},\\r\\npublisher={Public Library of Science},\\r\\nissn={19326203},\\r\\ncoden={POLNC},\\r\\npubmed_id={28961258},\\r\\nlanguage={English},\\r\\nabbrev_source_title={PLoS ONE},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Makarov, E.\",\"Shtam, T.\",\"Kovalev, R.\",\"Pantina, R.\",\"Varfolomeeva, E.\",\"Filatov, M.\"],\"key\":\"Makarov2017\",\"id\":\"Makarov2017\",\"bibbaseid\":\"makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&doi=10.1371%2fjournal.pone.0185126&partnerID=40&md5=e33272f5dbd1aae05eb33bbe72e9eea2\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Iashina\"],\"firstnames\":[\"E.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Velichko\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bouwman\"],\"firstnames\":[\"W.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Duif\"],\"firstnames\":[\"C.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Brulet\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grigoriev\"],\"firstnames\":[\"S.V.\"],\"suffixes\":[]}],\"title\":\"Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei\",\"journal\":\"Physical Review E\",\"year\":\"2017\",\"volume\":\"96\",\"number\":\"1\",\"doi\":\"10.1103/PhysRevE.96.012411\",\"art_number\":\"012411\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&doi=10.1103%2fPhysRevE.96.012411&partnerID=40&md5=e15ead8307ee2efbb140f35ce40ca971\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Gatchina, St.-Petersburg, 188300, Russian Federation; Saint Petersburg State University, Ulyanovskaya 1, St.-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, JB Delft, 2629, Netherlands; Leon Brillouin Laboratory, CEA Saclay, Gif sur Yvette Cedex, 91191, France\",\"abstract\":\"Small-angle neutron scattering (SANS) on nuclei of chicken erythrocytes demonstrates the cubic dependence of the scattering intensity Q-3 in the range of momentum transfer Q 10-3-10-2nm-1. Independent spin-echo SANS measurements give the spin-echo function, which is well described by the exponential law in a range of sizes (3×102)-(3×104) nm. Both experimental dependences reflect the nature of the structural organization of chromatin in the nucleus of a living cell, which corresponds to the correlation function γ(r)=ln(ξ/r) for r<ξ, where ξ=(3.69±0.07)×103 nm, the size of the nucleus. It has the specific scaling property of the logarithmic fractal γ(r/a)=γ(r)+ln(a), i.e., the scaling down by a gives an additive constant to the correlation function, which distinguishes it from the mass fractal, which is characterized by multiplicative constant. © 2017 American Physical Society.\",\"publisher\":\"American Physical Society\",\"issn\":\"24700045\",\"pubmed_id\":\"29347273\",\"language\":\"English\",\"abbrev_source_title\":\"Phys. Rev. E\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Iashina2017,\\r\\nauthor={Iashina, E.G. and Velichko, E.V. and Filatov, M.V. and Bouwman, W.G. and Duif, C.P. and Brulet, A. and Grigoriev, S.V.},\\r\\ntitle={Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei},\\r\\njournal={Physical Review E},\\r\\nyear={2017},\\r\\nvolume={96},\\r\\nnumber={1},\\r\\ndoi={10.1103/PhysRevE.96.012411},\\r\\nart_number={012411},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&doi=10.1103%2fPhysRevE.96.012411&partnerID=40&md5=e15ead8307ee2efbb140f35ce40ca971},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, St.-Petersburg, 188300, Russian Federation; Saint Petersburg State University, Ulyanovskaya 1, St.-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, JB Delft, 2629, Netherlands; Leon Brillouin Laboratory, CEA Saclay, Gif sur Yvette Cedex, 91191, France},\\r\\nabstract={Small-angle neutron scattering (SANS) on nuclei of chicken erythrocytes demonstrates the cubic dependence of the scattering intensity Q-3 in the range of momentum transfer Q 10-3-10-2nm-1. Independent spin-echo SANS measurements give the spin-echo function, which is well described by the exponential law in a range of sizes (3×102)-(3×104) nm. Both experimental dependences reflect the nature of the structural organization of chromatin in the nucleus of a living cell, which corresponds to the correlation function γ(r)=ln(ξ/r) for r<ξ, where ξ=(3.69±0.07)×103 nm, the size of the nucleus. It has the specific scaling property of the logarithmic fractal γ(r/a)=γ(r)+ln(a), i.e., the scaling down by a gives an additive constant to the correlation function, which distinguishes it from the mass fractal, which is characterized by multiplicative constant. © 2017 American Physical Society.},\\r\\npublisher={American Physical Society},\\r\\nissn={24700045},\\r\\npubmed_id={29347273},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Phys. Rev. E},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Iashina, E.\",\"Velichko, E.\",\"Filatov, M.\",\"Bouwman, W.\",\"Duif, C.\",\"Brulet, A.\",\"Grigoriev, S.\"],\"key\":\"Iashina2017\",\"id\":\"Iashina2017\",\"bibbaseid\":\"iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&doi=10.1103%2fPhysRevE.96.012411&partnerID=40&md5=e15ead8307ee2efbb140f35ce40ca971\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Iashina\"],\"firstnames\":[\"E.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bouwman\"],\"firstnames\":[\"W.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Duif\"],\"firstnames\":[\"C.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grigoriev\"],\"firstnames\":[\"S.V.\"],\"suffixes\":[]}],\"title\":\"Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell\",\"journal\":\"Journal of Physics: Conference Series\",\"year\":\"2017\",\"volume\":\"862\",\"number\":\"1\",\"doi\":\"10.1088/1742-6596/862/1/012010\",\"art_number\":\"012010\",\"note\":\"cited By 0; Conference of 11th International Conference on Polarised Neutrons for Condensed Matter Investigations, PNCMI 2016 ; Conference Date: 4 July 2016 Through 7 July 2016; Conference Code:128574\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&doi=10.1088%2f1742-6596%2f862%2f1%2f012010&partnerID=40&md5=33d0632e7d097bc7f05412a5d0883c38\",\"affiliation\":\"Petersburg Nuclear Physics Institute NRC KI, Gatchina, St-Petersburg, 188300, Russian Federation; Saint-Petersburg State University, Saint-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, Delft, 2629 JB, Netherlands; Department of Physics, University of Bristol, Tyndalls Park Road, Bristol, BS8 1TS, United Kingdom\",\"abstract\":\"Spin-echo small-angle scattering (SESANS) technique is a method to measure the structure of materials from nano- to micrmeter length scales. This method could be important for studying the packaging of DNA in the eukaryotic cell. We measured the SESANS function from chicken erythrocyte nuclei which is well fitted by the exponential function G(z) = exp(-z/ξ), where ξ is the correlation length of a nucleus (in experimental data ξ = 3, 3 μm). The exponential decay of G(z) corresponds to the logarithmic pair correlation function γ(r) = ln(ξ/r). As the sensitivity of the SESANS signal depends on the neutron wavelength, we propose the SESANS setup with the changeable wavelength in the range from 2 to 12 Å. Such option allows one to study in great detail the internal structure of the biological cell in the length scale from 10-2 μm to 10 μm. © Published under licence by IOP Publishing Ltd.\",\"sponsors\":\"AIRBUS DS; et al.; ISIS; J-PARC; LLB; Swiss Neutronics\",\"publisher\":\"Institute of Physics Publishing\",\"issn\":\"17426588\",\"language\":\"English\",\"abbrev_source_title\":\"J. Phys. Conf. Ser.\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Iashina2017,\\r\\nauthor={Iashina, E.G. and Bouwman, W.G. and Duif, C.P. and Filatov, M.V. and Grigoriev, S.V.},\\r\\ntitle={Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell},\\r\\njournal={Journal of Physics: Conference Series},\\r\\nyear={2017},\\r\\nvolume={862},\\r\\nnumber={1},\\r\\ndoi={10.1088/1742-6596/862/1/012010},\\r\\nart_number={012010},\\r\\nnote={cited By 0; Conference of 11th International Conference on Polarised Neutrons for Condensed Matter Investigations, PNCMI 2016 ; Conference Date: 4 July 2016 Through 7 July 2016;  Conference Code:128574},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&doi=10.1088%2f1742-6596%2f862%2f1%2f012010&partnerID=40&md5=33d0632e7d097bc7f05412a5d0883c38},\\r\\naffiliation={Petersburg Nuclear Physics Institute NRC KI, Gatchina, St-Petersburg, 188300, Russian Federation; Saint-Petersburg State University, Saint-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, Delft, 2629 JB, Netherlands; Department of Physics, University of Bristol, Tyndalls Park Road, Bristol, BS8 1TS, United Kingdom},\\r\\nabstract={Spin-echo small-angle scattering (SESANS) technique is a method to measure the structure of materials from nano- to micrmeter length scales. This method could be important for studying the packaging of DNA in the eukaryotic cell. We measured the SESANS function from chicken erythrocyte nuclei which is well fitted by the exponential function G(z) = exp(-z/ξ), where ξ is the correlation length of a nucleus (in experimental data ξ = 3, 3 μm). The exponential decay of G(z) corresponds to the logarithmic pair correlation function γ(r) = ln(ξ/r). As the sensitivity of the SESANS signal depends on the neutron wavelength, we propose the SESANS setup with the changeable wavelength in the range from 2 to 12 Å. Such option allows one to study in great detail the internal structure of the biological cell in the length scale from 10-2 μm to 10 μm. © Published under licence by IOP Publishing Ltd.},\\r\\nsponsors={AIRBUS DS; et al.; ISIS; J-PARC; LLB; Swiss Neutronics},\\r\\npublisher={Institute of Physics Publishing},\\r\\nissn={17426588},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J. Phys. Conf. Ser.},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Iashina, E.\",\"Bouwman, W.\",\"Duif, C.\",\"Filatov, M.\",\"Grigoriev, S.\"],\"key\":\"Iashina2017-1\",\"id\":\"Iashina2017-1\",\"bibbaseid\":\"iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&doi=10.1088%2f1742-6596%2f862%2f1%2f012010&partnerID=40&md5=33d0632e7d097bc7f05412a5d0883c38\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bairamukov\"],\"firstnames\":[\"V.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malek\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Orlov\"],\"firstnames\":[\"Y.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2017\",\"volume\":\"11\",\"number\":\"2\",\"pages\":\"172-179\",\"doi\":\"10.1134/S1990519X17020043\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&doi=10.1134%2fS1990519X17020043&partnerID=40&md5=f439cee2262ea4552aa9c299648a59e1\",\"affiliation\":\"National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Peter the Great Polytechnic University, St. Petersburg, 195251, Russian Federation; Petrov Institute of Oncology, Ministry of Healthcare of the Russian Federation, St. Petersburg, 197758, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russian Federation\",\"abstract\":\"Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.\",\"author_keywords\":\"exosomes; lectins; methods of exosome isolation\",\"correspondence_address1\":\"Filatov, M.V.; National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil_53@mail.ru\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2017172,\\r\\nauthor={Shtam, T.A. and Burdakov, V.S. and Landa, S.B. and Naryzhny, S.N. and Bairamukov, V.Y. and Malek, A.V. and Orlov, Y.N. and Filatov, M.V.},\\r\\ntitle={Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2017},\\r\\nvolume={11},\\r\\nnumber={2},\\r\\npages={172-179},\\r\\ndoi={10.1134/S1990519X17020043},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&doi=10.1134%2fS1990519X17020043&partnerID=40&md5=f439cee2262ea4552aa9c299648a59e1},\\r\\naffiliation={National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Peter the Great Polytechnic University, St. Petersburg, 195251, Russian Federation; Petrov Institute of Oncology, Ministry of Healthcare of the Russian Federation, St. Petersburg, 197758, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russian Federation},\\r\\nabstract={Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={exosomes;  lectins;  methods of exosome isolation},\\r\\ncorrespondence_address1={Filatov, M.V.; National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil_53@mail.ru},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Burdakov, V.\",\"Landa, S.\",\"Naryzhny, S.\",\"Bairamukov, V.\",\"Malek, A.\",\"Orlov, Y.\",\"Filatov, M.\"],\"key\":\"Shtam2017172\",\"id\":\"Shtam2017172\",\"bibbaseid\":\"shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&doi=10.1134%2fS1990519X17020043&partnerID=40&md5=f439cee2262ea4552aa9c299648a59e1\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]}],\"title\":\"Quaternary structures of human cytoplasmic and nuclear PCNA are the same\",\"journal\":\"Biomeditsinskaya Khimiya\",\"year\":\"2017\",\"volume\":\"63\",\"number\":\"4\",\"pages\":\"356-360\",\"doi\":\"10.18097/PBMC20176304356\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&doi=10.18097%2fPBMC20176304356&partnerID=40&md5=ce5cadb78677efafb3f40f9a9cf8706b\",\"affiliation\":\"Petersburg Nuclear Physics Institute NRC Kurchatov Institute, Leningrad Region (PNPI), Gatchina, 188300, Russian Federation\",\"abstract\":\"Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-to-back principle (Naryzhny S.N. et al. (2005) J. Biol. Chem., 280, 13888). Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well.\",\"author_keywords\":\"Cytoplasm; Double trimer; Monomer; Neutrophil; PCNA; Structure\",\"publisher\":\"Russian Academy of Medical Sciences\",\"issn\":\"23106905\",\"pubmed_id\":\"28862608\",\"language\":\"Russian\",\"abbrev_source_title\":\"Biomeditsinskaya Khim.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Belyakova2017356,\\r\\nauthor={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},\\r\\ntitle={Quaternary structures of human cytoplasmic and nuclear PCNA are the same},\\r\\njournal={Biomeditsinskaya Khimiya},\\r\\nyear={2017},\\r\\nvolume={63},\\r\\nnumber={4},\\r\\npages={356-360},\\r\\ndoi={10.18097/PBMC20176304356},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&doi=10.18097%2fPBMC20176304356&partnerID=40&md5=ce5cadb78677efafb3f40f9a9cf8706b},\\r\\naffiliation={Petersburg Nuclear Physics Institute NRC Kurchatov Institute, Leningrad Region (PNPI), Gatchina, 188300, Russian Federation},\\r\\nabstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-to-back principle (Naryzhny S.N. et al. (2005) J. Biol. Chem., 280, 13888). Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well.},\\r\\nauthor_keywords={Cytoplasm;  Double trimer;  Monomer;  Neutrophil;  PCNA;  Structure},\\r\\npublisher={Russian Academy of Medical Sciences},\\r\\nissn={23106905},\\r\\npubmed_id={28862608},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Biomeditsinskaya Khim.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Belyakova, N.\",\"Pantina, R.\",\"Kovalev, R.\",\"Filatov, M.\",\"Naryzhny, S.\"],\"key\":\"Belyakova2017356\",\"id\":\"Belyakova2017356\",\"bibbaseid\":\"belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&doi=10.18097%2fPBMC20176304356&partnerID=40&md5=ce5cadb78677efafb3f40f9a9cf8706b\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Samsonov\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Radzhabovа\"],\"firstnames\":[\"Z.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vasilyev\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tsyrlina\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Titov\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Berstein\"],\"firstnames\":[\"L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kolesnikov\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gil-Henn\"],\"firstnames\":[\"H.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malek\"],\"firstnames\":[\"A.\"],\"suffixes\":[]}],\"title\":\"Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer\",\"journal\":\"Tumor Biology\",\"year\":\"2016\",\"volume\":\"37\",\"number\":\"9\",\"pages\":\"12011-12021\",\"doi\":\"10.1007/s13277-016-5065-3\",\"note\":\"cited By 29\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&doi=10.1007%2fs13277-016-5065-3&partnerID=40&md5=44202226962197cb45fd5d86662beabe\",\"affiliation\":\"Oncosystem Ltd, Hoshimina 11/1-207, Saint-Petersburg, 194356, Russian Federation; NN Petrov Institute of Oncology, Leningradskaya 68, Saint-Petersburg, 197758, Russian Federation; FSBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, 195251, Russian Federation; Institute of Molecular and Cellular Biology SB RAS, Lavrentieva 8/2, Novosibirsk, 630090, Russian Federation; Faculty of Medicine in the Galilee, Bar-Ilan University, Henrietta Szold 8, Safed, 13100, Israel\",\"abstract\":\"Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over the last few decades. As has been revealed by a number of studies, TC tissue’s micro-RNA (miRNA) profile may reflect histological features and the clinical behavior of tumor. However, alteration of the miRNA profile of plasma exosomes associated with TC development has to date not been explored. We isolated exosomes from plasma and assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, and western blotting. Next, we profiled cancer-associated miRNAs in plasma exosomes obtained from papillary TC patients, before and after surgical removal of the tumor. The diagnostic value of selected miRNAs was evaluated in a large cohort of patients displaying different statuses of thyroid nodule disease. MiRNA assessment was performed by RT-qPCR. In total, 60 patients with different types of thyroid nodal pathology were included in the study. Our results revealed that the development of papillary TC is associated with specific changes in exosomal miRNA profiles; this phenomenon can be used for differential diagnostics. MiRNA-31 was found to be over-represented in the plasma exosomes of patients with papillary TC vs. benign tumors, while miRNA-21 helped to distinguish between benign tumors and follicular TC. MiRNA-21 and MiRNA-181a-5p were found to be expressed reciprocally in the exosomes of patients with papillary and follicular TC, and their comparative assessment may help to distinguish between these types of TC with 100 % sensitivity and 77 % specificity. © 2016, International Society of Oncology and BioMarkers (ISOBM).\",\"author_keywords\":\"Diagnostics; Exosomes; MicroRNA; Thyroid cancer\",\"correspondence_address1\":\"Malek, A.; Oncosystem Ltd, Hoshimina 11/1-207, Russian Federation; email: anastasia@malek.com\",\"publisher\":\"Springer Netherlands\",\"issn\":\"10104283\",\"coden\":\"TUMBE\",\"pubmed_id\":\"27164936\",\"language\":\"English\",\"abbrev_source_title\":\"Tumor Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Samsonov201612011,\\r\\nauthor={Samsonov, R. and Burdakov, V. and Shtam, T. and Radzhabovа, Z. and Vasilyev, D. and Tsyrlina, E. and Titov, S. and Ivanov, M. and Berstein, L. and Filatov, M. and Kolesnikov, N. and Gil-Henn, H. and Malek, A.},\\r\\ntitle={Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer},\\r\\njournal={Tumor Biology},\\r\\nyear={2016},\\r\\nvolume={37},\\r\\nnumber={9},\\r\\npages={12011-12021},\\r\\ndoi={10.1007/s13277-016-5065-3},\\r\\nnote={cited By 29},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&doi=10.1007%2fs13277-016-5065-3&partnerID=40&md5=44202226962197cb45fd5d86662beabe},\\r\\naffiliation={Oncosystem Ltd, Hoshimina 11/1-207, Saint-Petersburg, 194356, Russian Federation; NN Petrov Institute of Oncology, Leningradskaya 68, Saint-Petersburg, 197758, Russian Federation; FSBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg  188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, 195251, Russian Federation; Institute of Molecular and Cellular Biology SB RAS, Lavrentieva 8/2, Novosibirsk, 630090, Russian Federation; Faculty of Medicine in the Galilee, Bar-Ilan University, Henrietta Szold 8, Safed, 13100, Israel},\\r\\nabstract={Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over the last few decades. As has been revealed by a number of studies, TC tissue’s micro-RNA (miRNA) profile may reflect histological features and the clinical behavior of tumor. However, alteration of the miRNA profile of plasma exosomes associated with TC development has to date not been explored. We isolated exosomes from plasma and assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, and western blotting. Next, we profiled cancer-associated miRNAs in plasma exosomes obtained from papillary TC patients, before and after surgical removal of the tumor. The diagnostic value of selected miRNAs was evaluated in a large cohort of patients displaying different statuses of thyroid nodule disease. MiRNA assessment was performed by RT-qPCR. In total, 60 patients with different types of thyroid nodal pathology were included in the study. Our results revealed that the development of papillary TC is associated with specific changes in exosomal miRNA profiles; this phenomenon can be used for differential diagnostics. MiRNA-31 was found to be over-represented in the plasma exosomes of patients with papillary TC vs. benign tumors, while miRNA-21 helped to distinguish between benign tumors and follicular TC. MiRNA-21 and MiRNA-181a-5p were found to be expressed reciprocally in the exosomes of patients with papillary and follicular TC, and their comparative assessment may help to distinguish between these types of TC with 100 % sensitivity and 77 % specificity. © 2016, International Society of Oncology and BioMarkers (ISOBM).},\\r\\nauthor_keywords={Diagnostics;  Exosomes;  MicroRNA;  Thyroid cancer},\\r\\ncorrespondence_address1={Malek, A.; Oncosystem Ltd, Hoshimina 11/1-207, Russian Federation; email: anastasia@malek.com},\\r\\npublisher={Springer Netherlands},\\r\\nissn={10104283},\\r\\ncoden={TUMBE},\\r\\npubmed_id={27164936},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Tumor Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Samsonov, R.\",\"Burdakov, V.\",\"Shtam, T.\",\"Radzhabovа, Z.\",\"Vasilyev, D.\",\"Tsyrlina, E.\",\"Titov, S.\",\"Ivanov, M.\",\"Berstein, L.\",\"Filatov, M.\",\"Kolesnikov, N.\",\"Gil-Henn, H.\",\"Malek, A.\"],\"key\":\"Samsonov201612011\",\"id\":\"Samsonov201612011\",\"bibbaseid\":\"samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&doi=10.1007%2fs13277-016-5065-3&partnerID=40&md5=44202226962197cb45fd5d86662beabe\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sokolov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Aplin\"],\"firstnames\":[\"K.D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Timofeeva\"],\"firstnames\":[\"K.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vasilyev\"],\"firstnames\":[\"V.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Ceruloplasmin decreases respiratory burst reaction during pregnancy\",\"journal\":\"Free Radical Research\",\"year\":\"2016\",\"volume\":\"50\",\"number\":\"8\",\"pages\":\"909-919\",\"doi\":\"10.1080/10715762.2016.1197395\",\"note\":\"cited By 8\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&doi=10.1080%2f10715762.2016.1197395&partnerID=40&md5=e0176bf5319caea3ccbca3344954e1b4\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, SFBI “Petersburg Nuclear Physics Institute” (National Research Center “Kurchatov Institute”), Gatchina, Russian Federation; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, St. Petersburg, Russian Federation; Chair of Fundamental Problems of Medicine, Saint-Petersburg State University, St. Petersburg, Russian Federation\",\"abstract\":\"Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin – a copper-containing glycoprotein – is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy. © 2016 Informa UK Limited, trading as Taylor & Francis Group.\",\"author_keywords\":\"Ceruloplasmin; flow cytometry; neutrophils; pregnancy; respiratory burst reaction\",\"correspondence_address1\":\"Sokolov, A.V.; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, Acad. Pavlova str., 12, Russian Federation; email: biochemsokolov@gmail.com\",\"publisher\":\"Taylor and Francis Ltd\",\"issn\":\"10715762\",\"coden\":\"FRARE\",\"pubmed_id\":\"27266720\",\"language\":\"English\",\"abbrev_source_title\":\"Free Radic. Res.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Varfolomeeva2016909,\\r\\nauthor={Varfolomeeva, E.Y. and Semenova, E.V. and Sokolov, A.V. and Aplin, K.D. and Timofeeva, K.E. and Vasilyev, V.B. and Filatov, M.V.},\\r\\ntitle={Ceruloplasmin decreases respiratory burst reaction during pregnancy},\\r\\njournal={Free Radical Research},\\r\\nyear={2016},\\r\\nvolume={50},\\r\\nnumber={8},\\r\\npages={909-919},\\r\\ndoi={10.1080/10715762.2016.1197395},\\r\\nnote={cited By 8},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&doi=10.1080%2f10715762.2016.1197395&partnerID=40&md5=e0176bf5319caea3ccbca3344954e1b4},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, SFBI “Petersburg Nuclear Physics Institute” (National Research Center “Kurchatov Institute”), Gatchina, Russian Federation; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, St. Petersburg, Russian Federation; Chair of Fundamental Problems of Medicine, Saint-Petersburg State University, St. Petersburg, Russian Federation},\\r\\nabstract={Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin – a copper-containing glycoprotein – is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy. © 2016 Informa UK Limited, trading as Taylor & Francis Group.},\\r\\nauthor_keywords={Ceruloplasmin;  flow cytometry;  neutrophils;  pregnancy;  respiratory burst reaction},\\r\\ncorrespondence_address1={Sokolov, A.V.; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, Acad. Pavlova str., 12, Russian Federation; email: biochemsokolov@gmail.com},\\r\\npublisher={Taylor and Francis Ltd},\\r\\nissn={10715762},\\r\\ncoden={FRARE},\\r\\npubmed_id={27266720},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Free Radic. Res.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Varfolomeeva, E.\",\"Semenova, E.\",\"Sokolov, A.\",\"Aplin, K.\",\"Timofeeva, K.\",\"Vasilyev, V.\",\"Filatov, M.\"],\"key\":\"Varfolomeeva2016909\",\"id\":\"Varfolomeeva2016909\",\"bibbaseid\":\"varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&doi=10.1080%2f10715762.2016.1197395&partnerID=40&md5=e0176bf5319caea3ccbca3344954e1b4\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volnitsky\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2016\",\"volume\":\"10\",\"number\":\"4\",\"pages\":\"259-263\",\"doi\":\"10.1134/S1990519X16040118\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&doi=10.1134%2fS1990519X16040118&partnerID=40&md5=9aafccdeff858ecc1fc8db1b00051926\",\"affiliation\":\"Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation\",\"abstract\":\"Although progress has been made in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that glioma malignant transformation is accompanied by gradual loss of LGI1 gene expression. However, genetic defects underlying LGI1 inactivation are not recognized. In this work, we analyzed LGI1 gene expression in primary cultures of malignant gliomas and compared these data with posttranslational modifications of H3 histone, an epigenetic indicator of transcriptional activity. We showed the presence of an epigenetic marker of gene repression H3K9me3 near LGL1 transcription initiation site in most malignant gliomas (five out of six). No expression of LGI1 gene was registered in these gliomas. LGI1 expression was documented only in one glioma, and this tumor did not exhibit an association of LGI1 gene with H3K9me3 modification. Thus, we demonstrated for the first time a correlation between an LGI1 gene expression and epigenetic indicator H3K9met3 in malignant gliomas. An Н3К4ас marker of actively transcribed chromatin near LGI1 transcription initiation site was not found. These data suggest the inactivation of LGI1 gene through an epigenetic mechanism: a modified “histone code.” © 2016, Pleiades Publishing, Ltd.\",\"author_keywords\":\"epigenetic regulation; gliomas; histone code; LGI1 gene\",\"correspondence_address1\":\"Semenova, E.V.; Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov InstituteRussian Federation; email: semenova_el.spb@mail.ru\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2016259,\\r\\nauthor={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},\\r\\ntitle={Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2016},\\r\\nvolume={10},\\r\\nnumber={4},\\r\\npages={259-263},\\r\\ndoi={10.1134/S1990519X16040118},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&doi=10.1134%2fS1990519X16040118&partnerID=40&md5=9aafccdeff858ecc1fc8db1b00051926},\\r\\naffiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation},\\r\\nabstract={Although progress has been made in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that glioma malignant transformation is accompanied by gradual loss of LGI1 gene expression. However, genetic defects underlying LGI1 inactivation are not recognized. In this work, we analyzed LGI1 gene expression in primary cultures of malignant gliomas and compared these data with posttranslational modifications of H3 histone, an epigenetic indicator of transcriptional activity. We showed the presence of an epigenetic marker of gene repression H3K9me3 near LGL1 transcription initiation site in most malignant gliomas (five out of six). No expression of LGI1 gene was registered in these gliomas. LGI1 expression was documented only in one glioma, and this tumor did not exhibit an association of LGI1 gene with H3K9me3 modification. Thus, we demonstrated for the first time a correlation between an LGI1 gene expression and epigenetic indicator H3K9met3 in malignant gliomas. An Н3К4ас marker of actively transcribed chromatin near LGI1 transcription initiation site was not found. These data suggest the inactivation of LGI1 gene through an epigenetic mechanism: a modified “histone code.” © 2016, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={epigenetic regulation;  gliomas;  histone code;  LGI1 gene},\\r\\ncorrespondence_address1={Semenova, E.V.; Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov InstituteRussian Federation; email: semenova_el.spb@mail.ru},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Volnitsky, A.\",\"Filatov, M.\"],\"key\":\"Semenova2016259\",\"id\":\"Semenova2016259\",\"bibbaseid\":\"semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&doi=10.1134%2fS1990519X16040118&partnerID=40&md5=9aafccdeff858ecc1fc8db1b00051926\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volnitsky\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS\",\"journal\":\"Tsitologiia\",\"year\":\"2016\",\"volume\":\"58\",\"number\":\"5\",\"pages\":\"335-339\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&partnerID=40&md5=9d291f5873458153cdec69d5434ac722\",\"abstract\":\"Although there is a progress in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that process of malignant transformation of gliomas is accompanied by gradual loss of LGI1 gene expression. However, genetic defects causing LGI1 inactivation have not been revealed. In this paper, we have analyzed the LGI1 gene expression in primary cultures of malignant gliomas, and compared these data with epigenetic indicators of transcriptional activity — posttranslational H3 histone modifications. We have show the presence of an epigenetic marker of gene repression H3K9me3 near the site of LGI1 transcription initiation in most (5 from 6) studied gliomas. There was not LGI1 expression in these gliomas. Only one glioma showed LGI1 expression, and in this glioma there was no association of LGI1gene with H3K9me3 modification. Thus, we are the first to show a correlation between LGI1 gene expression and the epigenetic indicator H3K9met3 in malignant gliomas. Marker of actively transcribed chromatin Í3K4àñ have not been found in this area of the genome. The data obtained strongly suggest the possibility of gene LGL1 inactivation by epigenetic mechanism: modified «histone code».\",\"issn\":\"00413771\",\"pubmed_id\":\"30188624\",\"language\":\"English; Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2016335,\\r\\nauthor={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},\\r\\ntitle={THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS},\\r\\njournal={Tsitologiia},\\r\\nyear={2016},\\r\\nvolume={58},\\r\\nnumber={5},\\r\\npages={335-339},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&partnerID=40&md5=9d291f5873458153cdec69d5434ac722},\\r\\nabstract={Although there is a progress in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that process of malignant transformation of gliomas is accompanied by gradual loss of LGI1 gene expression. However, genetic defects causing LGI1 inactivation have not been revealed. In this paper, we have analyzed the LGI1 gene expression in primary cultures of malignant gliomas, and compared these data with epigenetic indicators of transcriptional activity — posttranslational H3 histone modifications. We have show the presence of an epigenetic marker of gene repression H3K9me3 near the site of LGI1 transcription initiation in most (5 from 6) studied gliomas. There was not LGI1 expression in these gliomas. Only one glioma showed LGI1 expression, and in this glioma there was no association of LGI1gene with H3K9me3 modification. Thus, we are the first to show a correlation between LGI1 gene expression and the epigenetic indicator H3K9met3 in malignant gliomas. Marker of actively transcribed chromatin Í3K4àñ have not been found in this area of the genome. The data obtained strongly suggest the possibility of gene LGL1 inactivation by epigenetic mechanism: modified «histone code».},\\r\\nissn={00413771},\\r\\npubmed_id={30188624},\\r\\nlanguage={English; Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Volnitsky, A.\",\"Filatov, M.\"],\"key\":\"Semenova2016335\",\"id\":\"Semenova2016335\",\"bibbaseid\":\"semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&partnerID=40&md5=9d291f5873458153cdec69d5434ac722\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Samsonov\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Glotov\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tsyrlina\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Berstein\"],\"firstnames\":[\"L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nosov\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Evtushenko\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malek\"],\"firstnames\":[\"A.\"],\"suffixes\":[]}],\"title\":\"Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic\",\"journal\":\"Prostate\",\"year\":\"2016\",\"volume\":\"76\",\"number\":\"1\",\"pages\":\"68-79\",\"doi\":\"10.1002/pros.23101\",\"note\":\"cited By 62\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&doi=10.1002%2fpros.23101&partnerID=40&md5=10835f69427cb4f1655bac5857581ea3\",\"affiliation\":\"Laboratory of Oncoendocrinology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Genetic Engineering, Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg, Russian Federation; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg, Russian Federation; Department of Genetics and Biotechnology, Saint Petersburg State University, Saint-Petersburg, Russian Federation; Department of Urology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Cell Migration and Invasion, Faculty of Medicine in the Galilee, Bar-Ilan University, Safed, Israel\",\"abstract\":\"BACKGROUND Prostate cancer is the most common cancer in men. Prostate-specific antigen has, however, insufficient diagnostic specificity. Novel complementary diagnostic approaches are greatly needed. MiRNAs are small regulatory RNAs which play an important role in tumorogenesis and are being investigated as a cancer biomarker. In addition to their intracellular regulatory functions, miRNAs are secreted into the extracellular space and can be found in various body fluids, including urine. The stability of extracellular miRNAs is defined by association with proteins, lipoprotein particles, and membrane vesicles. Among the known forms of miRNA packaging, tumour-derived exosome-enclosed miRNAs is thought to reflect the vital activity of cancer cells. The assessment of the exosomal fraction of urinary miRNA may present a new and highly specific method for prostate cancer diagnostics; however, this is challenged by the absence of reliable and inexpensive methods for isolation of exosomes. METHODS Prostate cancer (PC) cell lines and urine samples collected from 35 PC patients and 35 healthy donors were used in the study. Lectins, phytohemagglutinin, and concanavalin A were used to induce agglutination of exosomes. The efficiency of isolation process was evaluated by AFM and DLS assays. The protein content of isolated exosomes was analysed by western blotting. Exosomal RNA was assayed by automated electrophoresis and expression level of selected miRNAs was evaluated by RT-qPCR. The diagnostic potency of the urinary exosomal miRNA assessment was estimated by the ROC method. RESULTS The formation of multi-vesicular agglutinates in urine can be induced by incubation with lectin at a final concentration of 2 mg/ml. These agglutinates contain urinary exosomes and may be pelleted by centrifugation with a relatively low G-force. The analysis of PC-related miRNA in urinary exosomes revealed significant up-regulation of miR-574-3p, miR-141-5p, and miR-21-5p associated with PC. CONCLUSIONS Lectin-induced aggregation is a low-cost and easily performed method for isolation of exosomes from urine. Isolated exosomes can be further analysed in terms of miRNA content. The miRNA profile of urinary exosomes reflects development of prostate cancer and may present a promising diagnostic tool. Prostate 76:68-79, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.\",\"author_keywords\":\"diagnostic; exosomes; lectin; miRNA; prostate cancer\",\"correspondence_address1\":\"Malek, A.; NN Petrov Institute of Oncology, Leningradskaya 68, Russian Federation; email: anastasia@malek.com\",\"publisher\":\"John Wiley and Sons Inc.\",\"issn\":\"02704137\",\"coden\":\"PRSTD\",\"pubmed_id\":\"26417675\",\"language\":\"English\",\"abbrev_source_title\":\"Prostate\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Samsonov201668,\\r\\nauthor={Samsonov, R. and Shtam, T. and Burdakov, V. and Glotov, A. and Tsyrlina, E. and Berstein, L. and Nosov, A. and Evtushenko, V. and Filatov, M. and Malek, A.},\\r\\ntitle={Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic},\\r\\njournal={Prostate},\\r\\nyear={2016},\\r\\nvolume={76},\\r\\nnumber={1},\\r\\npages={68-79},\\r\\ndoi={10.1002/pros.23101},\\r\\nnote={cited By 62},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&doi=10.1002%2fpros.23101&partnerID=40&md5=10835f69427cb4f1655bac5857581ea3},\\r\\naffiliation={Laboratory of Oncoendocrinology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Genetic Engineering, Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg, Russian Federation; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg, Russian Federation; Department of Genetics and Biotechnology, Saint Petersburg State University, Saint-Petersburg, Russian Federation; Department of Urology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Cell Migration and Invasion, Faculty of Medicine in the Galilee, Bar-Ilan University, Safed, Israel},\\r\\nabstract={BACKGROUND Prostate cancer is the most common cancer in men. Prostate-specific antigen has, however, insufficient diagnostic specificity. Novel complementary diagnostic approaches are greatly needed. MiRNAs are small regulatory RNAs which play an important role in tumorogenesis and are being investigated as a cancer biomarker. In addition to their intracellular regulatory functions, miRNAs are secreted into the extracellular space and can be found in various body fluids, including urine. The stability of extracellular miRNAs is defined by association with proteins, lipoprotein particles, and membrane vesicles. Among the known forms of miRNA packaging, tumour-derived exosome-enclosed miRNAs is thought to reflect the vital activity of cancer cells. The assessment of the exosomal fraction of urinary miRNA may present a new and highly specific method for prostate cancer diagnostics; however, this is challenged by the absence of reliable and inexpensive methods for isolation of exosomes. METHODS Prostate cancer (PC) cell lines and urine samples collected from 35 PC patients and 35 healthy donors were used in the study. Lectins, phytohemagglutinin, and concanavalin A were used to induce agglutination of exosomes. The efficiency of isolation process was evaluated by AFM and DLS assays. The protein content of isolated exosomes was analysed by western blotting. Exosomal RNA was assayed by automated electrophoresis and expression level of selected miRNAs was evaluated by RT-qPCR. The diagnostic potency of the urinary exosomal miRNA assessment was estimated by the ROC method. RESULTS The formation of multi-vesicular agglutinates in urine can be induced by incubation with lectin at a final concentration of 2 mg/ml. These agglutinates contain urinary exosomes and may be pelleted by centrifugation with a relatively low G-force. The analysis of PC-related miRNA in urinary exosomes revealed significant up-regulation of miR-574-3p, miR-141-5p, and miR-21-5p associated with PC. CONCLUSIONS Lectin-induced aggregation is a low-cost and easily performed method for isolation of exosomes from urine. Isolated exosomes can be further analysed in terms of miRNA content. The miRNA profile of urinary exosomes reflects development of prostate cancer and may present a promising diagnostic tool. Prostate 76:68-79, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.},\\r\\nauthor_keywords={diagnostic;  exosomes;  lectin;  miRNA;  prostate cancer},\\r\\ncorrespondence_address1={Malek, A.; NN Petrov Institute of Oncology, Leningradskaya 68, Russian Federation; email: anastasia@malek.com},\\r\\npublisher={John Wiley and Sons Inc.},\\r\\nissn={02704137},\\r\\ncoden={PRSTD},\\r\\npubmed_id={26417675},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Prostate},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Samsonov, R.\",\"Shtam, T.\",\"Burdakov, V.\",\"Glotov, A.\",\"Tsyrlina, E.\",\"Berstein, L.\",\"Nosov, A.\",\"Evtushenko, V.\",\"Filatov, M.\",\"Malek, A.\"],\"key\":\"Samsonov201668\",\"id\":\"Samsonov201668\",\"bibbaseid\":\"samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&doi=10.1002%2fpros.23101&partnerID=40&md5=10835f69427cb4f1655bac5857581ea3\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Chernous\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shvets\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shagimuratov\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kalitenkov\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]}],\"title\":\"Studying navigation signal singularities during auroral disturbances\",\"journal\":\"Russian Journal of Physical Chemistry B\",\"year\":\"2015\",\"volume\":\"9\",\"number\":\"5\",\"pages\":\"778-784\",\"doi\":\"10.1134/S1990793115050188\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&doi=10.1134%2fS1990793115050188&partnerID=40&md5=77037f8ce87c89f036cb23c5eabf2ff7\",\"affiliation\":\"Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Western Division, Russian Academy of Sciences, pr. Pobedy 41, Kaliningrad, 236017, Russian Federation; Murmansk State Technical University, ul. Sportivnaya 13, Murmansk, 183010, Russian Federation\",\"abstract\":\"The experimental studies of the GPS and GLONASS satellite signal singularities, depending on the spatial and time distributions of auroras and geomagnetic disturbances that mark the polar ionosphere current state, are presented. The experimental evidences for positioning errors, related to the spatial–time variations in auroral arcs, are demonstrated. These errors of the navigation systems are observed in an increase in the positioning errors and in a violation of the system integrity. It has been indicated that auroral rayed arcs are indicators of disturbances in the navigation system operation. We tried to explain this fact in the scope of the concept of navigation signal phase fluctuations caused by total electron content inhomogeneities along geomagnetic field lines. © 2015, Pleiades Publishing, Ltd.\",\"author_keywords\":\"auroral disturbances; navigation systems; positioning error sources\",\"funding_details\":\"Russian Foundation for Basic Research4-05-98820r-sever-a\",\"correspondence_address1\":\"Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Russian Federation\",\"publisher\":\"Maik Nauka Publishing / Springer SBM\",\"issn\":\"19907931\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Phys. Chem. B\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Chernous2015778,\\r\\nauthor={Chernous, S.A. and Shvets, M.V. and Filatov, M.V. and Shagimuratov, I.I. and Kalitenkov, N.V.},\\r\\ntitle={Studying navigation signal singularities during auroral disturbances},\\r\\njournal={Russian Journal of Physical Chemistry B},\\r\\nyear={2015},\\r\\nvolume={9},\\r\\nnumber={5},\\r\\npages={778-784},\\r\\ndoi={10.1134/S1990793115050188},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&doi=10.1134%2fS1990793115050188&partnerID=40&md5=77037f8ce87c89f036cb23c5eabf2ff7},\\r\\naffiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Western Division, Russian Academy of Sciences, pr. Pobedy 41, Kaliningrad, 236017, Russian Federation; Murmansk State Technical University, ul. Sportivnaya 13, Murmansk, 183010, Russian Federation},\\r\\nabstract={The experimental studies of the GPS and GLONASS satellite signal singularities, depending on the spatial and time distributions of auroras and geomagnetic disturbances that mark the polar ionosphere current state, are presented. The experimental evidences for positioning errors, related to the spatial–time variations in auroral arcs, are demonstrated. These errors of the navigation systems are observed in an increase in the positioning errors and in a violation of the system integrity. It has been indicated that auroral rayed arcs are indicators of disturbances in the navigation system operation. We tried to explain this fact in the scope of the concept of navigation signal phase fluctuations caused by total electron content inhomogeneities along geomagnetic field lines. © 2015, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={auroral disturbances;  navigation systems;  positioning error sources},\\r\\nfunding_details={Russian Foundation for Basic Research14-07-00512},\\r\\nfunding_details={Russian Foundation for Basic Research4-05-98820r-sever-a},\\r\\ncorrespondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Russian Federation},\\r\\npublisher={Maik Nauka Publishing / Springer SBM},\\r\\nissn={19907931},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Phys. Chem. B},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Chernous, S.\",\"Shvets, M.\",\"Filatov, M.\",\"Shagimuratov, I.\",\"Kalitenkov, N.\"],\"key\":\"Chernous2015778\",\"id\":\"Chernous2015778\",\"bibbaseid\":\"chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&doi=10.1134%2fS1990793115050188&partnerID=40&md5=77037f8ce87c89f036cb23c5eabf2ff7\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Karelov\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volnitskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makarov\"],\"firstnames\":[\"E.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatova\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2015\",\"volume\":\"9\",\"number\":\"3\",\"pages\":\"191-197\",\"doi\":\"10.1134/S1990519X15030086\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&doi=10.1134%2fS1990519X15030086&partnerID=40&md5=0c901448296154db7453d31299ecdd32\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, St. Petersburg State Polytechnical University, St. Petersburg, 194064, Russian Federation; Division of Biosciences, Brunel University, London, United Kingdom\",\"abstract\":\"The p21/Waf1 protein is one of the main regulators of cell cycle arrest and one of the best-known transcriptional targets of the TP53 protein. Here, we demonstrated that there is activation of expression of the p21/Waf1 gene when the cells were treated with sodium butyrate (NaBu), which is a natural histone deacetylase inhibitor, and investigated whether this phenomenon depends on the presence of a functionally active TP53 protein. For this purpose, we compared the effect of NaBu treatment of human cell lines with different TP53 mutation profiles, including wild-type TP53, single nucleotide substitutions, and the complete absence of the TP53 gene. NaBu activated the TP53 protein via hyperacetylation at the lysine residue K382, without significant changes in the level of protein expression. Western blotting showed that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both TP53 wild-type cells and in cells with single nucleotide substitutions in the central DNA-binding core domain (DBD) of the TP53 protein. At the same time, no p21/Waf1 protein induction was observed in cells with complete deletion of the TP53 gene. However, NaBu was not able to induce p21/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein, but, unexpectedly, it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One possible explanations is that NaBu increases the level of TP53 acetylation and the modified protein is able to establish a new network of protein–protein interactions or trigger conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired. © 2015, Pleiades Publishing, Ltd.\",\"author_keywords\":\"HDAC inhibitors; p21/Waf1/Cip1; RNA interference; sodium butyrate; TP53; TP53 mutations\",\"correspondence_address1\":\"Filatova, M.V.; Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”Russian Federation\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kovalev2015191,\\r\\nauthor={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatova, M.V.},\\r\\ntitle={Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2015},\\r\\nvolume={9},\\r\\nnumber={3},\\r\\npages={191-197},\\r\\ndoi={10.1134/S1990519X15030086},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&doi=10.1134%2fS1990519X15030086&partnerID=40&md5=0c901448296154db7453d31299ecdd32},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, St. Petersburg State Polytechnical University, St. Petersburg, 194064, Russian Federation; Division of Biosciences, Brunel University, London, United Kingdom},\\r\\nabstract={The p21/Waf1 protein is one of the main regulators of cell cycle arrest and one of the best-known transcriptional targets of the TP53 protein. Here, we demonstrated that there is activation of expression of the p21/Waf1 gene when the cells were treated with sodium butyrate (NaBu), which is a natural histone deacetylase inhibitor, and investigated whether this phenomenon depends on the presence of a functionally active TP53 protein. For this purpose, we compared the effect of NaBu treatment of human cell lines with different TP53 mutation profiles, including wild-type TP53, single nucleotide substitutions, and the complete absence of the TP53 gene. NaBu activated the TP53 protein via hyperacetylation at the lysine residue K382, without significant changes in the level of protein expression. Western blotting showed that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both TP53 wild-type cells and in cells with single nucleotide substitutions in the central DNA-binding core domain (DBD) of the TP53 protein. At the same time, no p21/Waf1 protein induction was observed in cells with complete deletion of the TP53 gene. However, NaBu was not able to induce p21/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein, but, unexpectedly, it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One possible explanations is that NaBu increases the level of TP53 acetylation and the modified protein is able to establish a new network of protein–protein interactions or trigger conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired. © 2015, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={HDAC inhibitors;  p21/Waf1/Cip1;  RNA interference;  sodium butyrate;  TP53;  TP53 mutations},\\r\\ncorrespondence_address1={Filatova, M.V.; Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”Russian Federation},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kovalev, R.\",\"Shtam, T.\",\"Karelov, D.\",\"Burdakov, V.\",\"Volnitskiy, A.\",\"Makarov, E.\",\"Filatova, M.\"],\"key\":\"Kovalev2015191\",\"id\":\"Kovalev2015191\",\"bibbaseid\":\"kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&doi=10.1134%2fS1990519X15030086&partnerID=40&md5=0c901448296154db7453d31299ecdd32\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Morozkina\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gluzdikov\"],\"firstnames\":[\"I.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drozdov\"],\"firstnames\":[\"A.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Selivanov\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shavva\"],\"firstnames\":[\"A.G.\"],\"suffixes\":[]}],\"title\":\"Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens\",\"journal\":\"Russian Journal of Organic Chemistry\",\"year\":\"2015\",\"volume\":\"51\",\"number\":\"3\",\"pages\":\"411-416\",\"doi\":\"10.1134/S1070428015030215\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&doi=10.1134%2fS1070428015030215&partnerID=40&md5=911c9d5ff7da174e600a9815e94f60e9\",\"affiliation\":\"St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russian Federation; Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center, Orlova Roshcha, Gatchina, Leningrad oblast, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya ul. 29, St. Petersburg, 195251, Russian Federation\",\"abstract\":\"Sulfamates derived from estrogens of the 8α-series have been synthesized, and their effect on the growth of MCF-7 breast cancer cells has been studied. The best anticancer effect has been found for 7β-methyl-D-homo-6-oxa-8α-estrone sulfamate and 3-methoxy-2,17β-sulfamoyloxy-8α-estra-1,3,5(10)-triene which almost completely inhibit the growth of cancer cells. © 2015 Pleiades Publishing, Ltd.\",\"correspondence_address1\":\"Shavva, A.G.; St. Petersburg State University, Universitetskii pr. 26, Russian Federation\",\"publisher\":\"Maik Nauka Publishing / Springer SBM\",\"issn\":\"10704280\",\"coden\":\"RJOCE\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Org. Chem.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Morozkina2015411,\\r\\nauthor={Morozkina, S.N. and Gluzdikov, I.A. and Drozdov, A.S. and Selivanov, S.I. and Kovalev, R.A. and Filatov, M.V. and Shavva, A.G.},\\r\\ntitle={Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens},\\r\\njournal={Russian Journal of Organic Chemistry},\\r\\nyear={2015},\\r\\nvolume={51},\\r\\nnumber={3},\\r\\npages={411-416},\\r\\ndoi={10.1134/S1070428015030215},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&doi=10.1134%2fS1070428015030215&partnerID=40&md5=911c9d5ff7da174e600a9815e94f60e9},\\r\\naffiliation={St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russian Federation; Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center, Orlova Roshcha, Gatchina, Leningrad oblast, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya ul. 29, St. Petersburg, 195251, Russian Federation},\\r\\nabstract={Sulfamates derived from estrogens of the 8α-series have been synthesized, and their effect on the growth of MCF-7 breast cancer cells has been studied. The best anticancer effect has been found for 7β-methyl-D-homo-6-oxa-8α-estrone sulfamate and 3-methoxy-2,17β-sulfamoyloxy-8α-estra-1,3,5(10)-triene which almost completely inhibit the growth of cancer cells. © 2015 Pleiades Publishing, Ltd.},\\r\\ncorrespondence_address1={Shavva, A.G.; St. Petersburg State University, Universitetskii pr. 26, Russian Federation},\\r\\npublisher={Maik Nauka Publishing / Springer SBM},\\r\\nissn={10704280},\\r\\ncoden={RJOCE},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Org. Chem.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Morozkina, S.\",\"Gluzdikov, I.\",\"Drozdov, A.\",\"Selivanov, S.\",\"Kovalev, R.\",\"Filatov, M.\",\"Shavva, A.\"],\"key\":\"Morozkina2015411\",\"id\":\"Morozkina2015411\",\"bibbaseid\":\"morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&doi=10.1134%2fS1070428015030215&partnerID=40&md5=911c9d5ff7da174e600a9815e94f60e9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Karelov\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volnitskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makarov\"],\"firstnames\":[\"E.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53\",\"journal\":\"Tsitologiia\",\"year\":\"2015\",\"volume\":\"57\",\"number\":\"3\",\"pages\":\"204-211\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&partnerID=40&md5=bd66a7bb8e7e0ed78b3edf4820c210f3\",\"abstract\":\"p21/Waf1 protein is one of the main cell cycle arrest regulators and one of the most well-known transcriptional targets of TP53 protein. Here, we demonstrated the activation of expression of the p21/Waf1 gene when the cells were treated to sodium butyrate (NaBu)–one of the natural inhibitors of deacetylase, and investigated whether this phenomenon depends on the presence of functionally active TP53 protein. We compared the effect of the NaBu treatment on the human cell line with different TP53 mutation profile, including: wild-type TP53, single nucleotide substitutions, and the complete absence of TP53 gene. NaBu activated the TP53 protein via hyper acetylation at lysine residue K382, without significant changes in the level of protein expression. Western blotting demonstrated that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both the TP53 wild-type cells and in the cells with single nucleotide substitutions in the domain responsible for the binding of TP53 protein to DNA. At the same time, no the p21/Waf1 protein induction was observed in the cells with complete deletion of the TP53 gene. However, NaBu was not able to induce the p2 1/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that the NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein but unexpectedly it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One of the hypothetical explanations is that NaBu increases the level of TP53 acetylation, and the modified protein is able to establish a new network of protein-protein interactions or trigger some conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired.\",\"issn\":\"00413771\",\"pubmed_id\":\"26021170\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kovalev2015204,\\r\\nauthor={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatov, M.V.},\\r\\ntitle={Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53},\\r\\njournal={Tsitologiia},\\r\\nyear={2015},\\r\\nvolume={57},\\r\\nnumber={3},\\r\\npages={204-211},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&partnerID=40&md5=bd66a7bb8e7e0ed78b3edf4820c210f3},\\r\\nabstract={p21/Waf1 protein is one of the main cell cycle arrest regulators and one of the most well-known transcriptional targets of TP53 protein. Here, we demonstrated the activation of expression of the p21/Waf1 gene when the cells were treated to sodium butyrate (NaBu)--one of the natural inhibitors of deacetylase, and investigated whether this phenomenon depends on the presence of functionally active TP53 protein. We compared the effect of the NaBu treatment on the human cell line with different TP53 mutation profile, including: wild-type TP53, single nucleotide substitutions, and the complete absence of TP53 gene. NaBu activated the TP53 protein via hyper acetylation at lysine residue K382, without significant changes in the level of protein expression. Western blotting demonstrated that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both the TP53 wild-type cells and in the cells with single nucleotide substitutions in the domain responsible for the binding of TP53 protein to DNA. At the same time, no the p21/Waf1 protein induction was observed in the cells with complete deletion of the TP53 gene. However, NaBu was not able to induce the p2 1/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that the NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein but unexpectedly it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One of the hypothetical explanations is that NaBu increases the level of TP53 acetylation, and the modified protein is able to establish a new network of protein-protein interactions or trigger some conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired.},\\r\\nissn={00413771},\\r\\npubmed_id={26021170},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kovalev, R.\",\"Shtam, T.\",\"Karelov, D.\",\"Burdakov, V.\",\"Volnitskiy, A.\",\"Makarov, E.\",\"Filatov, M.\"],\"key\":\"Kovalev2015204\",\"id\":\"Kovalev2015204\",\"bibbaseid\":\"kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&partnerID=40&md5=bd66a7bb8e7e0ed78b3edf4820c210f3\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Malek\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Berstein\"],\"firstnames\":[\"L.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Belyaev\"],\"firstnames\":[\"A.M.\"],\"suffixes\":[]}],\"title\":\"Exosomal intercellular communications system and its role in the process of metastatic dissemination\",\"journal\":\"Voprosy Onkologii\",\"year\":\"2015\",\"volume\":\"60\",\"number\":\"4\",\"pages\":\"429-436\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&partnerID=40&md5=d9a9fcdb72d174dbbcca3823bb3967f2\",\"affiliation\":\"Ltd. Oncosystem, Russian Federation; N.N. Petrov Research Institute of Oncology, St. Petersburg, Russian Federation; Kurchatov Institute, Gatchina, Russian Federation\",\"abstract\":\"Metabolism and information between cells is the basis of the existence of any multicellular organism. Malfunction of the intercellular communication play an important and sometimes decisive role in the pathogenesis of the most diseases, including cancer. According to traditional views, functional integration of individual cells in tissues, organs and organ systems is mediated by the efficient work of regulatory systems: nervous, immune, endocrine. Over the past few years the attention of scientists is attracted the ability of cells to «communicate» with the help of nanoscale vesicular formations, or so-called exosomes. There are accumulated data that the cells of the most tissues secrete exosomes into the intercellular environment, after which, by means of stream ofblood or lymph, exosomes transferred to anatomically distant sites where they are accepted by the other cells. It is showed that the content of exosomes are not random and that vesicular transport may be targeted and to play a significant physiological and even «pathophysiological» role. The aim of this review is the analysis and integration of modern scientific data on the role of exosomes in the process of tumor progression and presentation of possible ways and methods of using these data in the practice of oncology.\",\"author_keywords\":\"Cancer; Exosomes; Intercellular contacts; Metastasis\",\"publisher\":\"Izdatel'stvo Meditsina\",\"issn\":\"05073758\",\"coden\":\"VOONA\",\"pubmed_id\":\"25552061\",\"language\":\"Russian\",\"abbrev_source_title\":\"Vopr. Onkol.\",\"document_type\":\"Review\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Malek2015429,\\r\\nauthor={Malek, A.V. and Berstein, L.M. and Filatov, M.V. and Belyaev, A.M.},\\r\\ntitle={Exosomal intercellular communications system and its role in the process of metastatic dissemination},\\r\\njournal={Voprosy Onkologii},\\r\\nyear={2015},\\r\\nvolume={60},\\r\\nnumber={4},\\r\\npages={429-436},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&partnerID=40&md5=d9a9fcdb72d174dbbcca3823bb3967f2},\\r\\naffiliation={Ltd. Oncosystem, Russian Federation; N.N. Petrov Research Institute of Oncology, St. Petersburg, Russian Federation; Kurchatov Institute, Gatchina, Russian Federation},\\r\\nabstract={Metabolism and information between cells is the basis of the existence of any multicellular organism. Malfunction of the intercellular communication play an important and sometimes decisive role in the pathogenesis of the most diseases, including cancer. According to traditional views, functional integration of individual cells in tissues, organs and organ systems is mediated by the efficient work of regulatory systems: nervous, immune, endocrine. Over the past few years the attention of scientists is attracted the ability of cells to «communicate» with the help of nanoscale vesicular formations, or so-called exosomes. There are accumulated data that the cells of the most tissues secrete exosomes into the intercellular environment, after which, by means of stream ofblood or lymph, exosomes transferred to anatomically distant sites where they are accepted by the other cells. It is showed that the content of exosomes are not random and that vesicular transport may be targeted and to play a significant physiological and even «pathophysiological» role. The aim of this review is the analysis and integration of modern scientific data on the role of exosomes in the process of tumor progression and presentation of possible ways and methods of using these data in the practice of oncology.},\\r\\nauthor_keywords={Cancer;  Exosomes;  Intercellular contacts;  Metastasis},\\r\\npublisher={Izdatel'stvo Meditsina},\\r\\nissn={05073758},\\r\\ncoden={VOONA},\\r\\npubmed_id={25552061},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Vopr. Onkol.},\\r\\ndocument_type={Review},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Malek, A.\",\"Berstein, L.\",\"Filatov, M.\",\"Belyaev, A.\"],\"key\":\"Malek2015429\",\"id\":\"Malek2015429\",\"bibbaseid\":\"malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&partnerID=40&md5=d9a9fcdb72d174dbbcca3823bb3967f2\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Volnitski\",\"id\":\"Volnitski\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Volnitskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Aberrant expression of the sox2 gene in malignant gliomas\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2014\",\"volume\":\"8\",\"number\":\"5\",\"pages\":\"368-373\",\"doi\":\"10.1134/S1990519X14050101\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&doi=10.1134%2fS1990519X14050101&partnerID=40&md5=761056e34f65f3402cadffe380c42b4e\",\"affiliation\":\"Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation\",\"abstract\":\"Both genetic and epigenetic changes underlie the mechanisms of tumor initiation and progression. In this study, we analyzed sox2 gene expression and its epigenetic changes in primary cultures of malignant gliomas. The sox2 expression was detected in most (74%) gliomas, but not in morphologically normal brain tissue. These facts point to relationships between the sox2 transcription activity and the process of glioma malignant transformation. It was demonstrated that association of different areas of the sox2 gene with important epigenetic markers—posttranslational modifications of H3 histone H3K4ac and H3K9met3—did not correlate with sox2 expression. However, it suggests stochastic regulation of sox2 gene expression in malignant gliomas. © 2014, Pleiades Publishing, Ltd.\",\"author_keywords\":\"aberrant gene expression; gliomas; posttranslational H3 histone modifications; Sox2\",\"correspondence_address1\":\"Filatov, M.V.; Konstantinov St. Petersburg Nuclear Physics InstituteRussian Federation\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Volnitskiy2014368,\\r\\nauthor={Volnitskiy, A.V. and Semenova, E.V. and Shtam, T.A. and Kovalev, R.A. and Filatov, M.V.},\\r\\ntitle={Aberrant expression of the sox2 gene in malignant gliomas},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2014},\\r\\nvolume={8},\\r\\nnumber={5},\\r\\npages={368-373},\\r\\ndoi={10.1134/S1990519X14050101},\\r\\nnote={cited By 3},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&doi=10.1134%2fS1990519X14050101&partnerID=40&md5=761056e34f65f3402cadffe380c42b4e},\\r\\naffiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\\r\\nabstract={Both genetic and epigenetic changes underlie the mechanisms of tumor initiation and progression. In this study, we analyzed sox2 gene expression and its epigenetic changes in primary cultures of malignant gliomas. The sox2 expression was detected in most (74%) gliomas, but not in morphologically normal brain tissue. These facts point to relationships between the sox2 transcription activity and the process of glioma malignant transformation. It was demonstrated that association of different areas of the sox2 gene with important epigenetic markers—posttranslational modifications of H3 histone H3K4ac and H3K9met3—did not correlate with sox2 expression. However, it suggests stochastic regulation of sox2 gene expression in malignant gliomas. © 2014, Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={aberrant gene expression;  gliomas;  posttranslational H3 histone modifications;  Sox2},\\r\\ncorrespondence_address1={Filatov, M.V.; Konstantinov St. Petersburg Nuclear Physics InstituteRussian Federation},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Volnitskiy, A.\",\"Semenova, E.\",\"Shtam, T.\",\"Kovalev, R.\",\"Filatov, M.\"],\"key\":\"Volnitskiy2014368\",\"id\":\"Volnitskiy2014368\",\"bibbaseid\":\"volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&doi=10.1134%2fS1990519X14050101&partnerID=40&md5=761056e34f65f3402cadffe380c42b4e\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ronzhina\"],\"firstnames\":[\"N.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mainskova\"],\"firstnames\":[\"M.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Development of barcode and proteome profiling of glioblastoma\",\"journal\":\"Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry\",\"year\":\"2014\",\"volume\":\"8\",\"number\":\"3\",\"pages\":\"243-251\",\"doi\":\"10.1134/S1990750814030111\",\"note\":\"cited By 7\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&doi=10.1134%2fS1990750814030111&partnerID=40&md5=b61f4ee9207a407172433dd9dd39a941\",\"affiliation\":\"Konstantinov Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute, Gatchina, 188300, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russian Federation\",\"abstract\":\"High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments, we used several glioblastoma cell lines to obtain proteomics information specific for this disease. 2DE separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us to detecting more than 600 protein spots and identifying more than 130 of them. Proteome profiles in normal and glioblastoma cell lines are very similar but levels of several proteins have prominent differences between norm and cancer. Among these proteins are alpha-enolase (ENOA-HUMAN), pyruvate kinase M1/M2 (KPYM-HUMAN), cofilin 1 (COF1-HUMAN), translationally-controlled tumor protein TCTP-HUMAN, annexin 1 (ANXA1-HUMAN), PCNA (PCNA-HUMAN), p53 (TP53-HUMAN) and others. Most interesting results were obtained about protein p53. Its level was dramatically up-regulated and enriched by multiple additional isoforms in all glioblastoma cell lines. An immunological analysis (Western blot) of three hub-proteins (p53, 14-3-3, PCNA) allowed us to creating the minimal barcode of glioblastoma cell lines. These preliminary data point to this barcode as a promising diagnostic tool for testing of the biological fluids from patients. © 2014 Pleiades Publishing, Ltd.\",\"author_keywords\":\"glioblastoma; p53; PCNA; proteomics\",\"funding_details\":\"11 04 01043\",\"key\":\"Naryzhny2014243\",\"id\":\"Naryzhny2014243\",\"bibbaseid\":\"anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&doi=10.1134%2fS1990750814030111&partnerID=40&md5=b61f4ee9207a407172433dd9dd39a941\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Naryzhny\",\"id\":\"Naryzhny\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Rolland\"],\"firstnames\":[\"Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Marighetti\"],\"firstnames\":[\"P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malinverno\"],\"firstnames\":[\"C.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Confalonieri\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Luise\"],\"firstnames\":[\"C.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ducano\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Palamidessi\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bisi\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kajiho\"],\"firstnames\":[\"H.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Troglio\"],\"firstnames\":[\"F.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dunn\"],\"firstnames\":[\"A.R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Oldani\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lanzetti\"],\"firstnames\":[\"L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"DiFiore\"],\"firstnames\":[\"P.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Disanza\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Scita\"],\"firstnames\":[\"G.\"],\"suffixes\":[]}],\"title\":\"The CDC42-interacting protein 4 controls epithelial cell cohesion and tumor dissemination\",\"journal\":\"Developmental Cell\",\"year\":\"2014\",\"volume\":\"30\",\"number\":\"5\",\"pages\":\"553-568\",\"doi\":\"10.1016/j.devcel.2014.08.006\",\"note\":\"cited By 29\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&doi=10.1016%2fj.devcel.2014.08.006&partnerID=40&md5=a22252e06e433a5220b557e968f8395f\",\"affiliation\":\"IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Via Adamello 16, Milan, 20139, Italy; Dipartimento di Oncologia Sperimentale, Istituto Europeo di Oncologia, Milan, 20141, Italy; Dipartimento di Scienze Oncologiche, Università degli Studi di Torino, Istituto per la Ricerca e la Cura del Cancro, Strada Provinciale 142, Turin, Candiolo, 10060, Italy; Dipartimento di Scienze della Salute, San Paolo, Università degli Studi di Milano, Milan, 20122, Italy; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States\",\"abstract\":\"The role of endocytic proteins and the molecular mechanisms underlying epithelial cell cohesion and tumor dissemination are not well understood. Here, we report that the endocytic F-BAR-containing CDC42-interacting protein 4 (CIP4) is required for ERBB2- and TGF-β1-induced cell scattering, breast cancer (BC) cell motility and invasion into 3D matrices, and conversion from ductal breast carcinoma insitu to invasive carcinoma in mouse xenograft models. CIP4 promotes the formation of an E-cadherin-CIP4-SRC complex that controls SRC activation, E-cadherin endocytosis, and localized phosphorylation of the myosin light chain kinase, thereby impinging on the actomyosin contractility required to generate tangential forces to break cell-cell junctions. CIP4 is upregulated in ERBB2-positive human BC, correlates with increased distant metastasis, and is an independent predictor of poor disease outcome in subsets of BC patients. Thus, it critically controls cell-cell cohesion and is required for the acquisition of an invasive phenotype in breast tumors. © 2014 Elsevier Inc.\",\"funding_details\":\"Fondazione Cariplo2011-0596, 2010-0737\",\"key\":\"Rolland2014553\",\"id\":\"Rolland2014553\",\"bibbaseid\":\"anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&doi=10.1016%2fj.devcel.2014.08.006&partnerID=40&md5=a22252e06e433a5220b557e968f8395f\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makarov\"],\"firstnames\":[\"E.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kil\"],\"firstnames\":[\"Y.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Exosomes are natural carriers of exogenous siRNA to human cells in vitro\",\"journal\":\"Cell Communication and Signaling\",\"year\":\"2013\",\"volume\":\"11\",\"number\":\"1\",\"doi\":\"10.1186/1478-811X-11-88\",\"art_number\":\"88\",\"note\":\"cited By 160\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&doi=10.1186%2f1478-811X-11-88&partnerID=40&md5=da53d624b8be3bfd1494f309cb115de5\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; School of Health Sciences and Social Care, Brunel University, Uxbridge UB8 3PH, United Kingdom; Department of Biophysics, St. Petersburg State Polytechnical University, St.-Petersburg 195251, Russian Federation\",\"abstract\":\"Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.\",\"author_keywords\":\"Cancer therapy; Drug delivery system; Exosomes; RAD51; RNA interference (RNAi)\",\"correspondence_address1\":\"Filatov, M.V.; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"1478811X\",\"pubmed_id\":\"24245560\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Commun. Signal.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2013,\\r\\nauthor={Shtam, T.A. and Kovalev, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Kil, Y.V. and Filatov, M.V.},\\r\\ntitle={Exosomes are natural carriers of exogenous siRNA to human cells in vitro},\\r\\njournal={Cell Communication and Signaling},\\r\\nyear={2013},\\r\\nvolume={11},\\r\\nnumber={1},\\r\\ndoi={10.1186/1478-811X-11-88},\\r\\nart_number={88},\\r\\nnote={cited By 160},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&doi=10.1186%2f1478-811X-11-88&partnerID=40&md5=da53d624b8be3bfd1494f309cb115de5},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; School of Health Sciences and Social Care, Brunel University, Uxbridge UB8 3PH, United Kingdom; Department of Biophysics, St. Petersburg State Polytechnical University, St.-Petersburg 195251, Russian Federation},\\r\\nabstract={Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.},\\r\\nauthor_keywords={Cancer therapy;  Drug delivery system;  Exosomes;  RAD51;  RNA interference (RNAi)},\\r\\ncorrespondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={1478811X},\\r\\npubmed_id={24245560},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Commun. Signal.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Kovalev, R.\",\"Varfolomeeva, E.\",\"Makarov, E.\",\"Kil, Y.\",\"Filatov, M.\"],\"key\":\"Shtam2013\",\"id\":\"Shtam2013\",\"bibbaseid\":\"shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&doi=10.1186%2f1478-811X-11-88&partnerID=40&md5=da53d624b8be3bfd1494f309cb115de5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Yung\"],\"firstnames\":[\"I.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy\",\"journal\":\"Journal of Surface Investigation\",\"year\":\"2013\",\"volume\":\"7\",\"number\":\"6\",\"pages\":\"1137-1142\",\"doi\":\"10.1134/S1027451013060414\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&doi=10.1134%2fS1027451013060414&partnerID=40&md5=90679420c913620df35e1385632cd253\",\"affiliation\":\"National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation\",\"abstract\":\"The distribution of replication origins in the nuclei of different cells is studied by confocal microscopy. Based on the obtained images, three-dimensional maps of the positions of the origin centers is constructed and the distribution functions of the pair distances between them are calculated. It is established that the distance distribution function for HeLa and glioma human cells is linear at sizes up to 2 μm, which indicates that the size of the origin system is close to 2. The amplitude of the distance distribution function at small sizes has a power dependence on the nucleus size and is inversely proportional to the nucleus volume to the power of 0.9. Thus, the replication-origin distribution in a nucleus cannot be described by a model with a single Hausdorff dimension in the whole range of sizes. © 2013 Pleiades Publishing, Ltd.\",\"correspondence_address1\":\"Yung, I. A.; National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation; email: Igor.Yung@rambler.ru\",\"issn\":\"10274510\",\"language\":\"English\",\"abbrev_source_title\":\"J. Surf. Invest.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Yung20131137,\\r\\nauthor={Yung, I.A. and Pantina, R.A. and Lebedev, D.V. and Filatov, M.V. and Isaev-Ivanov, V.V.},\\r\\ntitle={Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy},\\r\\njournal={Journal of Surface Investigation},\\r\\nyear={2013},\\r\\nvolume={7},\\r\\nnumber={6},\\r\\npages={1137-1142},\\r\\ndoi={10.1134/S1027451013060414},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&doi=10.1134%2fS1027451013060414&partnerID=40&md5=90679420c913620df35e1385632cd253},\\r\\naffiliation={National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation},\\r\\nabstract={The distribution of replication origins in the nuclei of different cells is studied by confocal microscopy. Based on the obtained images, three-dimensional maps of the positions of the origin centers is constructed and the distribution functions of the pair distances between them are calculated. It is established that the distance distribution function for HeLa and glioma human cells is linear at sizes up to 2 μm, which indicates that the size of the origin system is close to 2. The amplitude of the distance distribution function at small sizes has a power dependence on the nucleus size and is inversely proportional to the nucleus volume to the power of 0.9. Thus, the replication-origin distribution in a nucleus cannot be described by a model with a single Hausdorff dimension in the whole range of sizes. © 2013 Pleiades Publishing, Ltd.},\\r\\ncorrespondence_address1={Yung, I. A.; National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation; email: Igor.Yung@rambler.ru},\\r\\nissn={10274510},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J. Surf. Invest.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Yung, I.\",\"Pantina, R.\",\"Lebedev, D.\",\"Filatov, M.\",\"Isaev-Ivanov, V.\"],\"key\":\"Yung20131137\",\"id\":\"Yung20131137\",\"bibbaseid\":\"yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&doi=10.1134%2fS1027451013060414&partnerID=40&md5=90679420c913620df35e1385632cd253\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bruder\"],\"firstnames\":[\"J.T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Chen\"],\"firstnames\":[\"P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Thomas\"],\"firstnames\":[\"C.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konovalova\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ekberg\"],\"firstnames\":[\"G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ettyreddy\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"McVey\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gall\"],\"firstnames\":[\"J.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"King\"],\"firstnames\":[\"C.R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Brough\"],\"firstnames\":[\"D.E.\"],\"suffixes\":[]}],\"title\":\"Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors\",\"journal\":\"Journal of Virology\",\"year\":\"2013\",\"volume\":\"87\",\"number\":\"17\",\"pages\":\"9661-9671\",\"doi\":\"10.1128/JVI.00462-13\",\"note\":\"cited By 8\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&doi=10.1128%2fJVI.00462-13&partnerID=40&md5=d7ebaa5d3acd911b11d1eafd42e0592e\",\"affiliation\":\"GenVec, Inc., Gaithersburg, MD, United States; International AIDS Vaccine Initiative, AIDS Vaccine Design and Development Laboratory, Brooklyn Army Terminal, Brooklyn, NY, United States\",\"abstract\":\"We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior exposure to Ad5. However, hexon-modified Ad5 vectors displayed an approximate 10-fold growth defect in complementing cells, making potential vaccine costs unacceptably high. Replacing hypervariable regions (HVRs) 1, 2, 4, and 5 with the equivalent HVRs from Ad43 was sufficient to avoid Ad5 preexisting immunity and retain full vaccine potential. However, the resulting vector displayed the same growth defect as the hexon-modified vector carrying all 9 HVRs from Ad43. The growth defect is likely due to a defect in capsid assembly, since DNA replication and late protein accumulation were normal in these vectors. We determined that the hexon-modified vectors have a 32°C cold-sensitive phenotype and selected revertants that restored vector productivity. Genome sequencing identified a single base change resulting in a threonine-to-methionine amino acid substitution at the position equivalent to residue 342 of the wild-type protein. This mutation has a suppressor phenotype (SP), since cloning it into our Ad5 vector containing all nine hypervariable regions from Ad43, Ad5.H(43m-43), increased yields over the version without the SP mutation. This growth improvement was also shown for an Ad5-based hexon-modified vector that carried the hexon hypervariable regions of Ad48, indicating that the SP mutation may have broad applicability for improving the productivity of different hexon-modified vectors. © 2013, American Society for Microbiology.\",\"funding_details\":\"National Institutes of Health1R43 AI077309-01\",\"correspondence_address1\":\"Bruder, J.T.; GenVec, Inc., Gaithersburg, MD, United States; email: joetbruder@gmail.com\",\"issn\":\"0022538X\",\"coden\":\"JOVIA\",\"pubmed_id\":\"23824800\",\"language\":\"English\",\"abbrev_source_title\":\"J. Virol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Bruder20139661,\\r\\nauthor={Bruder, J.T. and Chen, P. and Semenova, E. and Thomas, C.A. and Konovalova, S. and Ekberg, G. and Ettyreddy, D. and McVey, D. and Gall, J.G. and King, C.R. and Brough, D.E.},\\r\\ntitle={Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors},\\r\\njournal={Journal of Virology},\\r\\nyear={2013},\\r\\nvolume={87},\\r\\nnumber={17},\\r\\npages={9661-9671},\\r\\ndoi={10.1128/JVI.00462-13},\\r\\nnote={cited By 8},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&doi=10.1128%2fJVI.00462-13&partnerID=40&md5=d7ebaa5d3acd911b11d1eafd42e0592e},\\r\\naffiliation={GenVec, Inc., Gaithersburg, MD, United States; International AIDS Vaccine Initiative, AIDS Vaccine Design and Development Laboratory, Brooklyn Army Terminal, Brooklyn, NY, United States},\\r\\nabstract={We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior exposure to Ad5. However, hexon-modified Ad5 vectors displayed an approximate 10-fold growth defect in complementing cells, making potential vaccine costs unacceptably high. Replacing hypervariable regions (HVRs) 1, 2, 4, and 5 with the equivalent HVRs from Ad43 was sufficient to avoid Ad5 preexisting immunity and retain full vaccine potential. However, the resulting vector displayed the same growth defect as the hexon-modified vector carrying all 9 HVRs from Ad43. The growth defect is likely due to a defect in capsid assembly, since DNA replication and late protein accumulation were normal in these vectors. We determined that the hexon-modified vectors have a 32°C cold-sensitive phenotype and selected revertants that restored vector productivity. Genome sequencing identified a single base change resulting in a threonine-to-methionine amino acid substitution at the position equivalent to residue 342 of the wild-type protein. This mutation has a suppressor phenotype (SP), since cloning it into our Ad5 vector containing all nine hypervariable regions from Ad43, Ad5.H(43m-43), increased yields over the version without the SP mutation. This growth improvement was also shown for an Ad5-based hexon-modified vector that carried the hexon hypervariable regions of Ad48, indicating that the SP mutation may have broad applicability for improving the productivity of different hexon-modified vectors. © 2013, American Society for Microbiology.},\\r\\nfunding_details={National Institutes of Health1R43 AI077309-01},\\r\\ncorrespondence_address1={Bruder, J.T.; GenVec, Inc., Gaithersburg, MD, United States; email: joetbruder@gmail.com},\\r\\nissn={0022538X},\\r\\ncoden={JOVIA},\\r\\npubmed_id={23824800},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J. Virol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Bruder, J.\",\"Chen, P.\",\"Semenova, E.\",\"Thomas, C.\",\"Konovalova, S.\",\"Ekberg, G.\",\"Ettyreddy, D.\",\"McVey, D.\",\"Gall, J.\",\"King, C.\",\"Brough, D.\"],\"key\":\"Bruder20139661\",\"id\":\"Bruder20139661\",\"bibbaseid\":\"bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&doi=10.1128%2fJVI.00462-13&partnerID=40&md5=d7ebaa5d3acd911b11d1eafd42e0592e\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Genetic and epigenetic markers of gliomas\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2013\",\"volume\":\"7\",\"number\":\"4\",\"pages\":\"303-313\",\"doi\":\"10.1134/S1990519X13040123\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&doi=10.1134%2fS1990519X13040123&partnerID=40&md5=d829132a07f3116bdb8a07052fe396d0\",\"affiliation\":\"Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation\",\"abstract\":\"Malignant gliomas are aggressive and highly invasive tumors. Various genetic and epigenetic changes are common for these tumors. Mostly they concern the genes involved in cell-cycle regulation, apoptotic pathways, cell invasion, angiogenesis, and cell metabolism. The role of epigenetic mechanisms in glioma malignant transformation, despite recent progress, is uncertain and remains under intense study. This review describes the mechanisms of epigenetic regulation of gene expression, including posttranslational modifications of histones, DNA methylation in promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used for diagnostics and new therapeutic approaches are also discussed. © 2013 Pleiades Publishing, Ltd.\",\"author_keywords\":\"epigenetic alterations; genetic particularities; gliomas\",\"correspondence_address1\":\"Semenova, E. V.; Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: semenova_el.spb@mail.ru\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Review\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2013303,\\r\\nauthor={Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Genetic and epigenetic markers of gliomas},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2013},\\r\\nvolume={7},\\r\\nnumber={4},\\r\\npages={303-313},\\r\\ndoi={10.1134/S1990519X13040123},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&doi=10.1134%2fS1990519X13040123&partnerID=40&md5=d829132a07f3116bdb8a07052fe396d0},\\r\\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},\\r\\nabstract={Malignant gliomas are aggressive and highly invasive tumors. Various genetic and epigenetic changes are common for these tumors. Mostly they concern the genes involved in cell-cycle regulation, apoptotic pathways, cell invasion, angiogenesis, and cell metabolism. The role of epigenetic mechanisms in glioma malignant transformation, despite recent progress, is uncertain and remains under intense study. This review describes the mechanisms of epigenetic regulation of gene expression, including posttranslational modifications of histones, DNA methylation in promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used for diagnostics and new therapeutic approaches are also discussed. © 2013 Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={epigenetic alterations;  genetic particularities;  gliomas},\\r\\ncorrespondence_address1={Semenova, E. V.; Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: semenova_el.spb@mail.ru},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Review},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Semenova2013303\",\"id\":\"Semenova2013303\",\"bibbaseid\":\"semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&doi=10.1134%2fS1990519X13040123&partnerID=40&md5=d829132a07f3116bdb8a07052fe396d0\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Babkina\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Garmai\"],\"firstnames\":[\"Y.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Using Zernike moments for analysis of images\",\"journal\":\"Numerical Analysis and Applications\",\"year\":\"2013\",\"volume\":\"6\",\"number\":\"2\",\"pages\":\"131-144\",\"doi\":\"10.1134/S1995423913020055\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&doi=10.1134%2fS1995423913020055&partnerID=40&md5=ae022d86a3cfca1a839a87e852e65b87\",\"affiliation\":\"Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Biophysics Scientific-Educational Structure at the St. Petersburg State Polytechnic University, Konstantinov Petersburg Nuclear Physics Institute, St. Petersburg, Russian Federation\",\"abstract\":\"A method for analyzing AFM images of the cell nuclei of higher organisms by expanding these images by Zernike moments is proposed. This method allows for expanding the pilot image by Zernike moments whose spatial harmonics are Zernike polynomials. It is shown that the reverse procedure of image reconstruction using Zernike polynomials converges to the experimental image and the expansion amplitude is a quantitative spectral characteristic in comparing the morphological features of different images. It is shown that expansion amplitudes can be used as input vectors for cluster analysis of images by PCA. © 2013 Pleiades Publishing, Ltd.\",\"author_keywords\":\"atomic force microscopy; cell nuclei of higher organisms; image analysis; PCA; Zernike moments\",\"funding_details\":\"Ministry of Education and Science of the Russian Federation2.2.1.1/1166\",\"bibtex\":\"@ARTICLE{Babkina2013131,\\r\\nauthor={Babkina, L.A. and Garmai, Y.P. and Lebedev, D.V. and Pantina, R.A. and Filatov, M.V. and Isaev-Ivanov, V.V.},\\r\\ntitle={Using Zernike moments for analysis of images},\\r\\njournal={Numerical Analysis and Applications},\\r\\nyear={2013},\\r\\nvolume={6},\\r\\nnumber={2},\\r\\npages={131-144},\\r\\ndoi={10.1134/S1995423913020055},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&doi=10.1134%2fS1995423913020055&partnerID=40&md5=ae022d86a3cfca1a839a87e852e65b87},\\r\\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Biophysics Scientific-Educational Structure at the St. Petersburg State Polytechnic University, Konstantinov Petersburg Nuclear Physics Institute, St. Petersburg, Russian Federation},\\r\\nabstract={A method for analyzing AFM images of the cell nuclei of higher organisms by expanding these images by Zernike moments is proposed. This method allows for expanding the pilot image by Zernike moments whose spatial harmonics are Zernike polynomials. It is shown that the reverse procedure of image reconstruction using Zernike polynomials converges to the experimental image and the expansion amplitude is a quantitative spectral characteristic in comparing the morphological features of different images. It is shown that expansion amplitudes can be used as input vectors for cluster analysis of images by PCA. © 2013 Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={atomic force microscopy;  cell nuclei of higher organisms;  image analysis;  PCA;  Zernike moments},\\r\\nfunding_details={Ministry of Education and Science of the Russian Federation2.2.1.1/1166},\\r\\n}\",\"author_short\":[\"Babkina, L.\",\"Garmai, Y.\",\"Lebedev, D.\",\"Pantina, R.\",\"Filatov, M.\",\"Isaev-Ivanov, V.\"],\"key\":\"Babkina2013131\",\"id\":\"Babkina2013131\",\"bibbaseid\":\"babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&doi=10.1134%2fS1995423913020055&partnerID=40&md5=ae022d86a3cfca1a839a87e852e65b87\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Genetic and epigenetic markers of gliomas\",\"journal\":\"Tsitologiya\",\"year\":\"2013\",\"volume\":\"55\",\"number\":\"5\",\"pages\":\"290-299\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&partnerID=40&md5=9827c4d438c05b1e4f0ec867aae49af4\",\"affiliation\":\"B. P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation\",\"abstract\":\"Malignant gliomas, aggressive and highly invasive tumors with a great number of genetic and epigenetic alterations in genes involved in the cell cycle regulation, the apoptotic pathways, cell invasion ability, and angiogenesis, are considered to be among the deadliest of human cancers. The role of epigenetic mechanisms in the pathogenesis of malignant transformation despite recent progress is not yet clear elucidated and remains under intensive study. This review describes the mechanisms of epigenetic regulation of gene expression, including post-translational modification of histones, DNA methylation in the promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used in the development of diagnostics and new therapeutic approaches are also discussed.\",\"author_keywords\":\"Epigenetic alterations; Genetic particularity; Gliomas\",\"publisher\":\"Maik Nauka Publishing / Springer SBM\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"24592735\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2013290,\\r\\nauthor={Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Genetic and epigenetic markers of gliomas},\\r\\njournal={Tsitologiya},\\r\\nyear={2013},\\r\\nvolume={55},\\r\\nnumber={5},\\r\\npages={290-299},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&partnerID=40&md5=9827c4d438c05b1e4f0ec867aae49af4},\\r\\naffiliation={B. P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},\\r\\nabstract={Malignant gliomas, aggressive and highly invasive tumors with a great number of genetic and epigenetic alterations in genes involved in the cell cycle regulation, the apoptotic pathways, cell invasion ability, and angiogenesis, are considered to be among the deadliest of human cancers. The role of epigenetic mechanisms in the pathogenesis of malignant transformation despite recent progress is not yet clear elucidated and remains under intensive study. This review describes the mechanisms of epigenetic regulation of gene expression, including post-translational modification of histones, DNA methylation in the promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used in the development of diagnostics and new therapeutic approaches are also discussed.},\\r\\nauthor_keywords={Epigenetic alterations;  Genetic particularity;  Gliomas},\\r\\npublisher={Maik Nauka Publishing / Springer SBM},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={24592735},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Semenova2013290\",\"id\":\"Semenova2013290\",\"bibbaseid\":\"semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&partnerID=40&md5=9827c4d438c05b1e4f0ec867aae49af4\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kovalev\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ibatullin\"],\"firstnames\":[\"F.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bondarev\"],\"firstnames\":[\"G.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"In vitro potential of histone deacetylase inhibitors for anticancer therapy\",\"journal\":\"Voprosy Onkologii\",\"year\":\"2012\",\"volume\":\"58\",\"number\":\"6\",\"pages\":\"800-807\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&partnerID=40&md5=330a4b66718da144b754fc8a404b3fd9\",\"affiliation\":\"Nuclear Physics Institute, Saint-Petersburg, Russian Federation\",\"abstract\":\"Research during the past decade has shown that epigenetic events have a key role in carcinogenesis and tumour progression. Histone deacetylase inhibitors (HDACi) comprise structurally diverse compounds that are a group of targeted epigenetic anticancer agents. Here we explored the in vitro efficacy of HDACi such as sodium butyrate (BuNa), valproic acid (VaNa) and several novel HDAC inhibitors for the treatment of cancer. Both BuNa and VaNa inhibited cancer cell proliferation in a time - and dose-dependent fashion. In the present study we demonstrated the significant effect of two novel HDACi, Adipo or BuNHOH, able to induce apoptosis of cancer cells, but not of normal line. Since HDAC inhibitors have been proposed as radio - or chemosensitizers in cancer therapy, we have studied the radiosensitizing effect of sodium butyrate on cancer cells. The combination of BuNa and radiation significantly inhibited tumor cell growth. Besides, combining Cisplatin or Gemzar with HDAC inhibitors results in synergistic antiproliferative activity that could be therapeutically exploited. These results suggest that HDACi acts as an antitumor agent and that combining HDAC inhibitors with radio or - chemotherapeutic strategy may provide a novel chemotherapeutic treatment of cancers insensitive to traditional antitumor agents.\",\"correspondence_address1\":\"Kovalev, R.A.; Nuclear Physics Institute, Saint-Petersburg, Russian Federation\",\"issn\":\"05073758\",\"coden\":\"VOONA\",\"pubmed_id\":\"23600307\",\"language\":\"Russian\",\"abbrev_source_title\":\"Vopr. Onkol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kovalev2012800,\\r\\nauthor={Kovalev, R.A. and Shtam, T.A. and Ibatullin, F.M. and Bondarev, G.N. and Filatov, M.V.},\\r\\ntitle={In vitro potential of histone deacetylase inhibitors for anticancer therapy},\\r\\njournal={Voprosy Onkologii},\\r\\nyear={2012},\\r\\nvolume={58},\\r\\nnumber={6},\\r\\npages={800-807},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&partnerID=40&md5=330a4b66718da144b754fc8a404b3fd9},\\r\\naffiliation={Nuclear Physics Institute, Saint-Petersburg, Russian Federation},\\r\\nabstract={Research during the past decade has shown that epigenetic events have a key role in carcinogenesis and tumour progression. Histone deacetylase inhibitors (HDACi) comprise structurally diverse compounds that are a group of targeted epigenetic anticancer agents. Here we explored the in vitro efficacy of HDACi such as sodium butyrate (BuNa), valproic acid (VaNa) and several novel HDAC inhibitors for the treatment of cancer. Both BuNa and VaNa inhibited cancer cell proliferation in a time - and dose-dependent fashion. In the present study we demonstrated the significant effect of two novel HDACi, Adipo or BuNHOH, able to induce apoptosis of cancer cells, but not of normal line. Since HDAC inhibitors have been proposed as radio - or chemosensitizers in cancer therapy, we have studied the radiosensitizing effect of sodium butyrate on cancer cells. The combination of BuNa and radiation significantly inhibited tumor cell growth. Besides, combining Cisplatin or Gemzar with HDAC inhibitors results in synergistic antiproliferative activity that could be therapeutically exploited. These results suggest that HDACi acts as an antitumor agent and that combining HDAC inhibitors with radio or - chemotherapeutic strategy may provide a novel chemotherapeutic treatment of cancers insensitive to traditional antitumor agents.},\\r\\ncorrespondence_address1={Kovalev, R.A.; Nuclear Physics Institute, Saint-Petersburg, Russian Federation},\\r\\nissn={05073758},\\r\\ncoden={VOONA},\\r\\npubmed_id={23600307},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Vopr. Onkol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kovalev, R.\",\"Shtam, T.\",\"Ibatullin, F.\",\"Bondarev, G.\",\"Filatov, M.\"],\"key\":\"Kovalev2012800\",\"id\":\"Kovalev2012800\",\"bibbaseid\":\"kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&partnerID=40&md5=330a4b66718da144b754fc8a404b3fd9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Volnyzky\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vinogradskaya\"],\"firstnames\":[\"G.R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"P73 gene expression in gliomas\",\"journal\":\"Voprosy Onkologii\",\"year\":\"2012\",\"volume\":\"58\",\"number\":\"4\",\"pages\":\"545-548\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&partnerID=40&md5=81ac9932d0f3ad831e25dbfd7c3c5d62\",\"affiliation\":\"B.P.Konstantinov Saint-Petersburg, Institute for Nuclear Physics, Russian Federation\",\"abstract\":\"We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.\",\"correspondence_address1\":\"Volnyzky, A.V.; B.P.Konstantinov Saint-Petersburg, Institute for Nuclear PhysicsRussian Federation\",\"issn\":\"05073758\",\"coden\":\"VOONA\",\"pubmed_id\":\"23607213\",\"language\":\"Russian\",\"abbrev_source_title\":\"Vopr. Onkol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Volnyzky2012545,\\r\\nauthor={Volnyzky, A.V. and Vinogradskaya, G.R. and Filatov, M.V.},\\r\\ntitle={P73 gene expression in gliomas},\\r\\njournal={Voprosy Onkologii},\\r\\nyear={2012},\\r\\nvolume={58},\\r\\nnumber={4},\\r\\npages={545-548},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&partnerID=40&md5=81ac9932d0f3ad831e25dbfd7c3c5d62},\\r\\naffiliation={B.P.Konstantinov Saint-Petersburg, Institute for Nuclear Physics, Russian Federation},\\r\\nabstract={We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.},\\r\\ncorrespondence_address1={Volnyzky, A.V.; B.P.Konstantinov Saint-Petersburg, Institute for Nuclear PhysicsRussian Federation},\\r\\nissn={05073758},\\r\\ncoden={VOONA},\\r\\npubmed_id={23607213},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Vopr. Onkol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Volnyzky, A.\",\"Vinogradskaya, G.\",\"Filatov, M.\"],\"key\":\"Volnyzky2012545\",\"id\":\"Volnyzky2012545\",\"bibbaseid\":\"volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&partnerID=40&md5=81ac9932d0f3ad831e25dbfd7c3c5d62\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ilatovskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Petukhov\"],\"firstnames\":[\"M.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Current insights into chromatin structure organization\",\"journal\":\"Tsitologiya\",\"year\":\"2012\",\"volume\":\"54\",\"number\":\"4\",\"pages\":\"298-306\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&partnerID=40&md5=fe62933099f2553f60478e65194b7c41\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation\",\"abstract\":\"This review summarizes current insights into organization of chromatin structure at different levels of DNA compaction. Analysis of available experimental data allowed concluding that only nucleosomal level of structural organization was sufficiently investigated, whereas structure of a 30-nm chromatin fiber remains an open issue. The data on the chromatin structure obtained at the level of the nucleus speak in favor of a biphasic fractal organization of chromatin.\",\"author_keywords\":\"30-nm fiber; Chromatin; DNA; Fractal; Nucleosome\",\"correspondence_address1\":\"Ilatovskiy, A.V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: andrcyi@omrb.pnpi.spb.ru\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"22724366\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Review\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ilatovskiy2012298,\\r\\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\\r\\ntitle={Current insights into chromatin structure organization},\\r\\njournal={Tsitologiya},\\r\\nyear={2012},\\r\\nvolume={54},\\r\\nnumber={4},\\r\\npages={298-306},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&partnerID=40&md5=fe62933099f2553f60478e65194b7c41},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},\\r\\nabstract={This review summarizes current insights into organization of chromatin structure at different levels of DNA compaction. Analysis of available experimental data allowed concluding that only nucleosomal level of structural organization was sufficiently investigated, whereas structure of a 30-nm chromatin fiber remains an open issue. The data on the chromatin structure obtained at the level of the nucleus speak in favor of a biphasic fractal organization of chromatin.},\\r\\nauthor_keywords={30-nm fiber;  Chromatin;  DNA;  Fractal;  Nucleosome},\\r\\ncorrespondence_address1={Ilatovskiy, A.V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: andrcyi@omrb.pnpi.spb.ru},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={22724366},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Review},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ilatovskiy, A.\",\"Lebedev, D.\",\"Filatov, M.\",\"Petukhov, M.\",\"Isaev-Ivanov, V.\"],\"key\":\"Ilatovskiy2012298\",\"id\":\"Ilatovskiy2012298\",\"bibbaseid\":\"ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&partnerID=40&md5=fe62933099f2553f60478e65194b7c41\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naiyzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdackov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Artamonova\"],\"firstnames\":[\"T.O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro\",\"journal\":\"Tsitologiya\",\"year\":\"2012\",\"volume\":\"54\",\"number\":\"5\",\"pages\":\"430-438\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&partnerID=40&md5=66c4733440532eaa83659d67c8673764\",\"affiliation\":\"B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation\",\"abstract\":\"Exosomes are 20-100 nm membrane vesicles of endocytic origin secreted by most cell types in vitro and in vivo. Since exosomes contain both RNA (mRNA and microRNA) and proteins, which can be transferred to another cell, and be functional in that new environment, these vesicles may be involved in the communication between cells. The secretion of exosomes by tumor cells and their implication in the transport and propagation of infectious cargo suggest their participation in pathological situations. Our purpose here is to describe methods for the production, purification, and proteomic characterization of exosomes derived from human cancer cells in vitro. Based on exosomes' unique lipidic composition, we have developed the new approach to increase production of exosomes by cells in vitro. Secondly, we have developed quality control by laser correlation spectroscopy for exosomal assays based on the amount of MHC class I and CD63 molecules on their surface. At last, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used after 2D electrophoresis for the proteomic analysis of exosomes derived from cancer cell lines. This study describes the protein composition of brain tumor cell-derived exosomes in more detail.\",\"author_keywords\":\"Exosomes; Laser correlation spectroscopy; Proteomic analysis\",\"correspondence_address1\":\"Filatov, M.V.; B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil53@mail.ru\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"22827041\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2012430,\\r\\nauthor={Shtam, T.A. and Naiyzhny, S.N. and Landa, S.B. and Burdackov, V.S. and Artamonova, T.O. and Filatov, M.V.},\\r\\ntitle={Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro},\\r\\njournal={Tsitologiya},\\r\\nyear={2012},\\r\\nvolume={54},\\r\\nnumber={5},\\r\\npages={430-438},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&partnerID=40&md5=66c4733440532eaa83659d67c8673764},\\r\\naffiliation={B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},\\r\\nabstract={Exosomes are 20-100 nm membrane vesicles of endocytic origin secreted by most cell types in vitro and in vivo. Since exosomes contain both RNA (mRNA and microRNA) and proteins, which can be transferred to another cell, and be functional in that new environment, these vesicles may be involved in the communication between cells. The secretion of exosomes by tumor cells and their implication in the transport and propagation of infectious cargo suggest their participation in pathological situations. Our purpose here is to describe methods for the production, purification, and proteomic characterization of exosomes derived from human cancer cells in vitro. Based on exosomes' unique lipidic composition, we have developed the new approach to increase production of exosomes by cells in vitro. Secondly, we have developed quality control by laser correlation spectroscopy for exosomal assays based on the amount of MHC class I and CD63 molecules on their surface. At last, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used after 2D electrophoresis for the proteomic analysis of exosomes derived from cancer cell lines. This study describes the protein composition of brain tumor cell-derived exosomes in more detail.},\\r\\nauthor_keywords={Exosomes;  Laser correlation spectroscopy;  Proteomic analysis},\\r\\ncorrespondence_address1={Filatov, M.V.; B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil53@mail.ru},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={22827041},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Naiyzhny, S.\",\"Landa, S.\",\"Burdackov, V.\",\"Artamonova, T.\",\"Filatov, M.\"],\"key\":\"Shtam2012430\",\"id\":\"Shtam2012430\",\"bibbaseid\":\"shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&partnerID=40&md5=66c4733440532eaa83659d67c8673764\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Borghi\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sorokina\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Weis\"],\"firstnames\":[\"W.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pruitt\"],\"firstnames\":[\"B.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nelson\"],\"firstnames\":[\"W.J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dunn\"],\"firstnames\":[\"A.R.\"],\"suffixes\":[]}],\"title\":\"Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109)\",\"journal\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"year\":\"2012\",\"volume\":\"109\",\"number\":\"46\",\"pages\":\"19034\",\"doi\":\"10.1073/pnas.1217417109\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&doi=10.1073%2fpnas.1217417109&partnerID=40&md5=893faf9b8234bde71083129c7163f3ff\",\"correspondence_address1\":\"Borghi, N.\",\"publisher\":\"National Academy of Sciences\",\"issn\":\"00278424\",\"coden\":\"PNASA\",\"language\":\"English\",\"abbrev_source_title\":\"Proc. Natl. Acad. Sci. U. S. A.\",\"document_type\":\"Erratum\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Borghi201219034,\\r\\nauthor={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},\\r\\ntitle={Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109)},\\r\\njournal={Proceedings of the National Academy of Sciences of the United States of America},\\r\\nyear={2012},\\r\\nvolume={109},\\r\\nnumber={46},\\r\\npages={19034},\\r\\ndoi={10.1073/pnas.1217417109},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&doi=10.1073%2fpnas.1217417109&partnerID=40&md5=893faf9b8234bde71083129c7163f3ff},\\r\\ncorrespondence_address1={Borghi, N.},\\r\\npublisher={National Academy of Sciences},\\r\\nissn={00278424},\\r\\ncoden={PNASA},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},\\r\\ndocument_type={Erratum},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Borghi, N.\",\"Sorokina, M.\",\"Shcherbakova, O.\",\"Weis, W.\",\"Pruitt, B.\",\"Nelson, W.\",\"Dunn, A.\"],\"key\":\"Borghi201219034\",\"id\":\"Borghi201219034\",\"bibbaseid\":\"borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&doi=10.1073%2fpnas.1217417109&partnerID=40&md5=893faf9b8234bde71083129c7163f3ff\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Soroka\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Korolev\"],\"firstnames\":[\"V.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagiian\"],\"firstnames\":[\"G.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Aplin\"],\"firstnames\":[\"K.D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gridasov\"],\"firstnames\":[\"G.G.\"],\"suffixes\":[]}],\"title\":\"[Absorption of iodofolic acids by the cells of malignant tumors].\",\"journal\":\"Bioorganicheskaia khimiia\",\"year\":\"2012\",\"volume\":\"38\",\"number\":\"6\",\"pages\":\"734-744\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&partnerID=40&md5=551a1ed8267ba2c6cffd52472fbc6df1\",\"abstract\":\"The uptake of 125-iodine labeled 3' iodofolic acid (I*F) and 3' iodo, 5 formyl tetrahydrofolic acid (I*FT) by the cells HeLa, ECV, L-41, human glioma, and rat glioma was studied. Human Embrionic Lung Fibroblasts (HELF) were taken for comparison as healthy cells. It was shown for *IF that its long-term uptake by cells L-41 and ECV is hundreds oftimes higher than those of HELF cells. The short-term uptake phase was studied for *IFT uptake. The dissociation constant was determined for a complex formed by *IFT and an acceptor in the HeLa cells, which is supposed to cause concentrative uptake of *IFT in cells. The dissociation constants of this acceptor complexes with folic acid, 3' iodofolic acid and 3',5'-diiodofolic acid were determined by competition with I*FT. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and rats with a sarcoma grafted on his thigh and glioma grafted into the brain was studied. As was shown there are large differences in the concentration of *IF and *IFT in the tumor and in the healthy tissue, *IF concentration in thigh muscle of healthy being 5 times lower than those in tumor grafted to the thigh, and *IFT concentration in healthy brain being 10 times lower than in brain tumor.\",\"correspondence_address1\":\"Soroka, N.V.\",\"issn\":\"01323423\",\"pubmed_id\":\"23547477\",\"language\":\"Russian\",\"abbrev_source_title\":\"Bioorg. Khim.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Soroka2012734,\\r\\nauthor={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiian, G.A. and Aplin, K.D. and Gridasov, G.G.},\\r\\ntitle={[Absorption of iodofolic acids by the cells of malignant tumors].},\\r\\njournal={Bioorganicheskaia khimiia},\\r\\nyear={2012},\\r\\nvolume={38},\\r\\nnumber={6},\\r\\npages={734-744},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&partnerID=40&md5=551a1ed8267ba2c6cffd52472fbc6df1},\\r\\nabstract={The uptake of 125-iodine labeled 3' iodofolic acid (I*F) and 3' iodo, 5 formyl tetrahydrofolic acid (I*FT) by the cells HeLa, ECV, L-41, human glioma, and rat glioma was studied. Human Embrionic Lung Fibroblasts (HELF) were taken for comparison as healthy cells. It was shown for *IF that its long-term uptake by cells L-41 and ECV is hundreds oftimes higher than those of HELF cells. The short-term uptake phase was studied for *IFT uptake. The dissociation constant was determined for a complex formed by *IFT and an acceptor in the HeLa cells, which is supposed to cause concentrative uptake of *IFT in cells. The dissociation constants of this acceptor complexes with folic acid, 3' iodofolic acid and 3',5'-diiodofolic acid were determined by competition with I*FT. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and rats with a sarcoma grafted on his thigh and glioma grafted into the brain was studied. As was shown there are large differences in the concentration of *IF and *IFT in the tumor and in the healthy tissue, *IF concentration in thigh muscle of healthy being 5 times lower than those in tumor grafted to the thigh, and *IFT concentration in healthy brain being 10 times lower than in brain tumor.},\\r\\ncorrespondence_address1={Soroka, N.V.},\\r\\nissn={01323423},\\r\\npubmed_id={23547477},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Bioorg. Khim.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Soroka, N.\",\"Filatov, M.\",\"Korolev, V.\",\"Bagiian, G.\",\"Aplin, K.\",\"Gridasov, G.\"],\"key\":\"Soroka2012734\",\"id\":\"Soroka2012734\",\"bibbaseid\":\"soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&partnerID=40&md5=551a1ed8267ba2c6cffd52472fbc6df1\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Soroka\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Korolev\"],\"firstnames\":[\"V.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagiyan\"],\"firstnames\":[\"G.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Aplin\"],\"firstnames\":[\"K.D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gridasov\"],\"firstnames\":[\"G.G.\"],\"suffixes\":[]}],\"title\":\"Absorption of iodofolic acids by the cells of malignant tumors\",\"journal\":\"Russian Journal of Bioorganic Chemistry\",\"year\":\"2012\",\"volume\":\"38\",\"number\":\"6\",\"pages\":\"652-661\",\"doi\":\"10.1134/S1068162012060131\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&doi=10.1134%2fS1068162012060131&partnerID=40&md5=f27639cceea9ebc8bc5cd19271332c20\",\"affiliation\":\"Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; Khlopin Radium Institute, Russian Federation\",\"abstract\":\"The uptake of our synthesized 125-iodine labeled 3'-iodofolic acid ( *IF) and 3'-iodo-5-formyltetrahyrofolic acid ( *IFT) by the HeLa, EVC, L-41, human glioma, and rat glioma cells has been studied. The Human Embryonic Lung Fibroblasts (HELFs) were used as healthy control cells for comparison. It was shown for the quantity of *IF absorbed at its long-term uptake phase by the L-41 and ECV cells is hundreds of times higher than that of the HELF cells. The short-term *IFT uptake phase by the cells was studied. For the HeLa cells, the dissociation constant was determined for the *IFT complex with an acceptor, which is supposed to be responsible for *IFT accumulation in the cells. Using competitive sorption approach, the dissociation constants of folic, 3'-iodofolic, and 3',5'-diiodofolic acid complexes with this acceptor were assessed. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and also in rats with sarcoma grafted on their thigh and glioma grafted into the brain was studied. A considerable differences were shown in the concentrations of *IF and *IFT in the tumor and in the healthy tissue, i.e., *IF in the healthy thigh muscle is five times lower than in the tumor grafted to the thigh, and *IFT concentration is 10 times lower in the healthy brain compared to the brain tumor. © Pleiades Publishing, Ltd., 2012.\",\"author_keywords\":\"Absorption; Folates; Iodine derivatives; Isotope 125I; Malignant cells\",\"correspondence_address1\":\"Filatov, M.V.; Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; email: fil53ster@gmail.ru\",\"issn\":\"10681620\",\"coden\":\"RJBCE\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Bioorg. Chem.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Soroka2012652,\\r\\nauthor={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiyan, G.A. and Aplin, K.D. and Gridasov, G.G.},\\r\\ntitle={Absorption of iodofolic acids by the cells of malignant tumors},\\r\\njournal={Russian Journal of Bioorganic Chemistry},\\r\\nyear={2012},\\r\\nvolume={38},\\r\\nnumber={6},\\r\\npages={652-661},\\r\\ndoi={10.1134/S1068162012060131},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&doi=10.1134%2fS1068162012060131&partnerID=40&md5=f27639cceea9ebc8bc5cd19271332c20},\\r\\naffiliation={Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; Khlopin Radium Institute, Russian Federation},\\r\\nabstract={The uptake of our synthesized 125-iodine labeled 3'-iodofolic acid ( *IF) and 3'-iodo-5-formyltetrahyrofolic acid ( *IFT) by the HeLa, EVC, L-41, human glioma, and rat glioma cells has been studied. The Human Embryonic Lung Fibroblasts (HELFs) were used as healthy control cells for comparison. It was shown for the quantity of *IF absorbed at its long-term uptake phase by the L-41 and ECV cells is hundreds of times higher than that of the HELF cells. The short-term *IFT uptake phase by the cells was studied. For the HeLa cells, the dissociation constant was determined for the *IFT complex with an acceptor, which is supposed to be responsible for *IFT accumulation in the cells. Using competitive sorption approach, the dissociation constants of folic, 3'-iodofolic, and 3',5'-diiodofolic acid complexes with this acceptor were assessed. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and also in rats with sarcoma grafted on their thigh and glioma grafted into the brain was studied. A considerable differences were shown in the concentrations of *IF and *IFT in the tumor and in the healthy tissue, i.e., *IF in the healthy thigh muscle is five times lower than in the tumor grafted to the thigh, and *IFT concentration is 10 times lower in the healthy brain compared to the brain tumor. © Pleiades Publishing, Ltd., 2012.},\\r\\nauthor_keywords={Absorption;  Folates;  Iodine derivatives;  Isotope 125I;  Malignant cells},\\r\\ncorrespondence_address1={Filatov, M.V.; Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; email: fil53ster@gmail.ru},\\r\\nissn={10681620},\\r\\ncoden={RJBCE},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Bioorg. Chem.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Soroka, N.\",\"Filatov, M.\",\"Korolev, V.\",\"Bagiyan, G.\",\"Aplin, K.\",\"Gridasov, G.\"],\"key\":\"Soroka2012652\",\"id\":\"Soroka2012652\",\"bibbaseid\":\"soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&doi=10.1134%2fS1068162012060131&partnerID=40&md5=f27639cceea9ebc8bc5cd19271332c20\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sun\"],\"firstnames\":[\"X.-L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yang\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Guan\"],\"firstnames\":[\"J.-P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ma\"],\"firstnames\":[\"Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Jiang\"],\"firstnames\":[\"J.-Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Cao\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burlyaeva\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vishnyakova\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bulyntsev\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zong\"],\"firstnames\":[\"X.-X.\"],\"suffixes\":[]}],\"title\":\"Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae)\",\"journal\":\"American Journal of Botany\",\"year\":\"2012\",\"volume\":\"99\",\"number\":\"10\",\"pages\":\"e379-e390\",\"doi\":\"10.3732/ajb.1100346\",\"note\":\"cited By 15\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&doi=10.3732%2fajb.1100346&partnerID=40&md5=16a3d02c0039ad7f415ea0468134678f\",\"affiliation\":\"Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Biological Sciences, China Agricultural University, Beijing 100193, China; Department of Leguminous Crops Genetic Resources, N. I. Vavilov Research Institute of Plant Industry, St. Petersburg 190000, Russian Federation\",\"abstract\":\"• Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. • Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. • Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea. © 2012 Botanical Society of America.\",\"author_keywords\":\"Expressed sequence tag; Fabaceae; Grasspea; Lathyrus sativus; Microsatellite; Primer\",\"correspondence_address1\":\"Zong, X.-X.; Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; email: zongxx@mail.caas.net.cn\",\"issn\":\"00029122\",\"coden\":\"AJBOA\",\"pubmed_id\":\"23028003\",\"language\":\"English\",\"abbrev_source_title\":\"Am. J. Bot.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Sun2012,\\r\\nauthor={Sun, X.-L. and Yang, T. and Guan, J.-P. and Ma, Y. and Jiang, J.-Y. and Cao, R. and Burlyaeva, M. and Vishnyakova, M. and Semenova, E. and Bulyntsev, S. and Zong, X.-X.},\\r\\ntitle={Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae)},\\r\\njournal={American Journal of Botany},\\r\\nyear={2012},\\r\\nvolume={99},\\r\\nnumber={10},\\r\\npages={e379-e390},\\r\\ndoi={10.3732/ajb.1100346},\\r\\nnote={cited By 15},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&doi=10.3732%2fajb.1100346&partnerID=40&md5=16a3d02c0039ad7f415ea0468134678f},\\r\\naffiliation={Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Biological Sciences, China Agricultural University, Beijing 100193, China; Department of Leguminous Crops Genetic Resources, N. I. Vavilov Research Institute of Plant Industry, St. Petersburg 190000, Russian Federation},\\r\\nabstract={• Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. • Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. • Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea. © 2012 Botanical Society of America.},\\r\\nauthor_keywords={Expressed sequence tag;  Fabaceae;  Grasspea;  Lathyrus sativus;  Microsatellite;  Primer},\\r\\ncorrespondence_address1={Zong, X.-X.; Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; email: zongxx@mail.caas.net.cn},\\r\\nissn={00029122},\\r\\ncoden={AJBOA},\\r\\npubmed_id={23028003},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Am. J. Bot.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Sun, X.\",\"Yang, T.\",\"Guan, J.\",\"Ma, Y.\",\"Jiang, J.\",\"Cao, R.\",\"Burlyaeva, M.\",\"Vishnyakova, M.\",\"Semenova, E.\",\"Bulyntsev, S.\",\"Zong, X.\"],\"key\":\"Sun2012\",\"id\":\"Sun2012\",\"bibbaseid\":\"sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&doi=10.3732%2fajb.1100346&partnerID=40&md5=16a3d02c0039ad7f415ea0468134678f\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Borghi\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sorokina\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Weis\"],\"firstnames\":[\"W.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pruitt\"],\"firstnames\":[\"B.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nelson\"],\"firstnames\":[\"W.J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dunn\"],\"firstnames\":[\"A.R.\"],\"suffixes\":[]}],\"title\":\"E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch\",\"journal\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"year\":\"2012\",\"volume\":\"109\",\"number\":\"31\",\"pages\":\"12568-12573\",\"doi\":\"10.1073/pnas.1204390109\",\"note\":\"cited By 284\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&doi=10.1073%2fpnas.1204390109&partnerID=40&md5=27a25f91d046dadaaa32776ddecb5cfa\",\"affiliation\":\"Department of Biology, Stanford University, Stanford, CA 94305, United States; Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, United States; Department of Cell Biology, Centre National de la Recherche Scientifique, Université Paris-Diderot, Paris 75013, France\",\"abstract\":\"Classical cadherins are transmembrane proteins at the core of intercellular adhesion complexes in cohesive metazoan tissues. The extracellular domain of classical cadherins forms intercellular bonds with cadherins on neighboring cells, whereas the cytoplasmic domain recruits catenins, which in turn associate with additional cytoskeleton binding and regulatory proteins. Cadherin/catenin complexes are hypothesized to play a role in the transduction of mechanical forces that shape cells and tissues during development, regeneration, and disease. Whether mechanical forces are transduced directly through cadherins is unknown. To address this question, we used a Förster resonance energy transfer (FRET)-based molecular tension sensor to test the origin and magnitude of tensile forces transmitted through the cytoplasmic domain of E-cadherin in epithelial cells. We show that the actomyosin cytoskeleton exerts pN-tensile force on E-cadherin, and that this tension requires the catenin-binding domain of E-cadherin and αE-catenin. Surprisingly, the actomyosin cytoskeleton constitutively exerts tension on E-cadherin at the plasma membrane regardless of whether or not E-cadherin is recruited to cell-cell contacts, although tension is further increased at cell-cell contacts when adhering cells are stretched. Our findings thus point to a constitutive role of E-cadherin in transducing mechanical forces between the actomyosin cytoskeleton and the plasmamembrane, not only at cell-cell junctions but throughout the cell surface.\",\"author_keywords\":\"Mechanobiology; Mechanosensor; Mechanotransduction; Morphogenesis; Signal transduction\",\"correspondence_address1\":\"Dunn, A.R.; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; email: alex.dunn@stanford.edu\",\"publisher\":\"National Academy of Sciences\",\"issn\":\"00278424\",\"coden\":\"PNASA\",\"pubmed_id\":\"22802638\",\"language\":\"English\",\"abbrev_source_title\":\"Proc. Natl. Acad. Sci. U. S. A.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Borghi201212568,\\r\\nauthor={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},\\r\\ntitle={E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch},\\r\\njournal={Proceedings of the National Academy of Sciences of the United States of America},\\r\\nyear={2012},\\r\\nvolume={109},\\r\\nnumber={31},\\r\\npages={12568-12573},\\r\\ndoi={10.1073/pnas.1204390109},\\r\\nnote={cited By 284},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&doi=10.1073%2fpnas.1204390109&partnerID=40&md5=27a25f91d046dadaaa32776ddecb5cfa},\\r\\naffiliation={Department of Biology, Stanford University, Stanford, CA 94305, United States; Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, United States; Department of Cell Biology, Centre National de la Recherche Scientifique, Université Paris-Diderot, Paris 75013, France},\\r\\nabstract={Classical cadherins are transmembrane proteins at the core of intercellular adhesion complexes in cohesive metazoan tissues. The extracellular domain of classical cadherins forms intercellular bonds with cadherins on neighboring cells, whereas the cytoplasmic domain recruits catenins, which in turn associate with additional cytoskeleton binding and regulatory proteins. Cadherin/catenin complexes are hypothesized to play a role in the transduction of mechanical forces that shape cells and tissues during development, regeneration, and disease. Whether mechanical forces are transduced directly through cadherins is unknown. To address this question, we used a Förster resonance energy transfer (FRET)-based molecular tension sensor to test the origin and magnitude of tensile forces transmitted through the cytoplasmic domain of E-cadherin in epithelial cells. We show that the actomyosin cytoskeleton exerts pN-tensile force on E-cadherin, and that this tension requires the catenin-binding domain of E-cadherin and αE-catenin. Surprisingly, the actomyosin cytoskeleton constitutively exerts tension on E-cadherin at the plasma membrane regardless of whether or not E-cadherin is recruited to cell-cell contacts, although tension is further increased at cell-cell contacts when adhering cells are stretched. Our findings thus point to a constitutive role of E-cadherin in transducing mechanical forces between the actomyosin cytoskeleton and the plasmamembrane, not only at cell-cell junctions but throughout the cell surface.},\\r\\nauthor_keywords={Mechanobiology;  Mechanosensor;  Mechanotransduction;  Morphogenesis;  Signal transduction},\\r\\ncorrespondence_address1={Dunn, A.R.; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; email: alex.dunn@stanford.edu},\\r\\npublisher={National Academy of Sciences},\\r\\nissn={00278424},\\r\\ncoden={PNASA},\\r\\npubmed_id={22802638},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Borghi, N.\",\"Sorokina, M.\",\"Shcherbakova, O.\",\"Weis, W.\",\"Pruitt, B.\",\"Nelson, W.\",\"Dunn, A.\"],\"key\":\"Borghi201212568\",\"id\":\"Borghi201212568\",\"bibbaseid\":\"borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&doi=10.1073%2fpnas.1204390109&partnerID=40&md5=27a25f91d046dadaaa32776ddecb5cfa\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Burdakov\"],\"firstnames\":[\"V.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Artamonova\"],\"firstnames\":[\"T.O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2012\",\"volume\":\"6\",\"number\":\"4\",\"pages\":\"317-325\",\"doi\":\"10.1134/S1990519X12040116\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&doi=10.1134%2fS1990519X12040116&partnerID=40&md5=621ea006f96d0bd38461fec3a60fde94\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation\",\"abstract\":\"Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that quantitative and qualitative characterization of these microparticles produced by different tissues in normal and pathological states can give valuable diagnostic and prognostic information and be a biomarker of different diseases, including oncological ones. Elaboration of the purification of exosomes and their proteome analysis was the aim of the present work. An original approach to enhancing exosome production in cultured transformed human cells was developed. The data obtained allowed us to detect exosomes in cultural conditioned samples and control the quality of produced exosomes at all stages of their purification. Electrophoretic analysis of proteins obtained from exosomes of different origins shows differences in protein profiles. Proteins from exosomes of glioblastoma cell lines were separated by two-dimensional electrophoresis. Protein profiles were further analyzed by densitometry and mass spectrometry, which allowed more than 30 proteins, including specific tumor markers, to be identified. © 2012 Pleiades Publishing, Ltd.\",\"author_keywords\":\"exosomes; laser correlation spectroscopy; proteome analysis\",\"correspondence_address1\":\"Filatov, M. V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil_53@mail.ru\",\"issn\":\"1990519X\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Tissue Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2012317,\\r\\nauthor={Shtam, T.A. and Naryzhny, S.N. and Landa, S.B. and Burdakov, V.S. and Artamonova, T.O. and Filatov, M.V.},\\r\\ntitle={Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells},\\r\\njournal={Cell and Tissue Biology},\\r\\nyear={2012},\\r\\nvolume={6},\\r\\nnumber={4},\\r\\npages={317-325},\\r\\ndoi={10.1134/S1990519X12040116},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&doi=10.1134%2fS1990519X12040116&partnerID=40&md5=621ea006f96d0bd38461fec3a60fde94},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\\r\\nabstract={Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that quantitative and qualitative characterization of these microparticles produced by different tissues in normal and pathological states can give valuable diagnostic and prognostic information and be a biomarker of different diseases, including oncological ones. Elaboration of the purification of exosomes and their proteome analysis was the aim of the present work. An original approach to enhancing exosome production in cultured transformed human cells was developed. The data obtained allowed us to detect exosomes in cultural conditioned samples and control the quality of produced exosomes at all stages of their purification. Electrophoretic analysis of proteins obtained from exosomes of different origins shows differences in protein profiles. Proteins from exosomes of glioblastoma cell lines were separated by two-dimensional electrophoresis. Protein profiles were further analyzed by densitometry and mass spectrometry, which allowed more than 30 proteins, including specific tumor markers, to be identified. © 2012 Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={exosomes;  laser correlation spectroscopy;  proteome analysis},\\r\\ncorrespondence_address1={Filatov, M. V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil_53@mail.ru},\\r\\nissn={1990519X},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Tissue Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Naryzhny, S.\",\"Landa, S.\",\"Burdakov, V.\",\"Artamonova, T.\",\"Filatov, M.\"],\"key\":\"Shtam2012317\",\"id\":\"Shtam2012317\",\"bibbaseid\":\"shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&doi=10.1134%2fS1990519X12040116&partnerID=40&md5=621ea006f96d0bd38461fec3a60fde94\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sim\"],\"firstnames\":[\"J.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Borghi\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ribeiro\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sorokina\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ramallo\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dunn\"],\"firstnames\":[\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nelson\"],\"firstnames\":[\"W.J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pruitt\"],\"firstnames\":[\"B.L.\"],\"suffixes\":[]}],\"title\":\"Uniaxial cell stretcher enables high resolution live cell imaging\",\"journal\":\"Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)\",\"year\":\"2012\",\"pages\":\"854-857\",\"doi\":\"10.1109/MEMSYS.2012.6170320\",\"art_number\":\"6170320\",\"note\":\"cited By 2; Conference of 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference Date: 29 January 2012 Through 2 February 2012; Conference Code:89445\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&doi=10.1109%2fMEMSYS.2012.6170320&partnerID=40&md5=10bc083a2824dc630c10ffed9ff11ea6\",\"affiliation\":\"Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; Biology, Stanford University, CA, United States; Chemical Engineering, Stanford University, CA, United States\",\"abstract\":\"Imaging adherent cells cultured on commercially available stretchable substrates presents challenges for high-magnification objectives. Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson's contraction with stretching, and issues of optical transparency or non-matching refractive indices. Beyond the advantages of visualizing biological structures in detail, we seek to implement specialized high-resolution fluorescence microscopy techniques such as Förster Resonance Energy Transfer (FRET) microscopy while stretching. Enabling FRET imaging of stretched cells provides a powerful tool for modern cell biology and mechanobiology [1]. FRET techniques require high magnification as well as a stable focal plane for imaging. Here, we address this requirement for stretchable substrates with a microfabricated cell strain device suitable for live cell imaging while allowing high-resolution FRET microscopy of cells. We adapt a uniaxial cell stretching concept previously demonstrated by Huh [2] for high magnification imaging by using thin bottom channels and membranes supported above an inverted oil immersion objective. This work presents a major advance for research in cell mechanobiology as it enables direct mechanical actuation combined with imaging of FRET probes engineered to report strained proteins in live cells. © 2012 IEEE.\",\"correspondence_address1\":\"Sim, J.Y.; Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; email: simba85@stanford.edu\",\"sponsors\":\"Robotics and Automation Society; IEEE; Region Nord-Pas de Calais\",\"address\":\"Paris\",\"issn\":\"10846999\",\"isbn\":\"9781467303248\",\"coden\":\"PMEME\",\"language\":\"English\",\"abbrev_source_title\":\"Proc. IEEE Int. Conf. Micro Electro Mech. Syst. MEMS\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Sim2012854,\\r\\nauthor={Sim, J.Y. and Borghi, N. and Ribeiro, A. and Sorokina, M. and Shcherbakova, O. and Ramallo, D. and Dunn, A. and Nelson, W.J. and Pruitt, B.L.},\\r\\ntitle={Uniaxial cell stretcher enables high resolution live cell imaging},\\r\\njournal={Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)},\\r\\nyear={2012},\\r\\npages={854-857},\\r\\ndoi={10.1109/MEMSYS.2012.6170320},\\r\\nart_number={6170320},\\r\\nnote={cited By 2; Conference of 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference Date: 29 January 2012 Through 2 February 2012;  Conference Code:89445},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&doi=10.1109%2fMEMSYS.2012.6170320&partnerID=40&md5=10bc083a2824dc630c10ffed9ff11ea6},\\r\\naffiliation={Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; Biology, Stanford University, CA, United States; Chemical Engineering, Stanford University, CA, United States},\\r\\nabstract={Imaging adherent cells cultured on commercially available stretchable substrates presents challenges for high-magnification objectives. Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson's contraction with stretching, and issues of optical transparency or non-matching refractive indices. Beyond the advantages of visualizing biological structures in detail, we seek to implement specialized high-resolution fluorescence microscopy techniques such as Förster Resonance Energy Transfer (FRET) microscopy while stretching. Enabling FRET imaging of stretched cells provides a powerful tool for modern cell biology and mechanobiology [1]. FRET techniques require high magnification as well as a stable focal plane for imaging. Here, we address this requirement for stretchable substrates with a microfabricated cell strain device suitable for live cell imaging while allowing high-resolution FRET microscopy of cells. We adapt a uniaxial cell stretching concept previously demonstrated by Huh [2] for high magnification imaging by using thin bottom channels and membranes supported above an inverted oil immersion objective. This work presents a major advance for research in cell mechanobiology as it enables direct mechanical actuation combined with imaging of FRET probes engineered to report strained proteins in live cells. © 2012 IEEE.},\\r\\ncorrespondence_address1={Sim, J.Y.; Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; email: simba85@stanford.edu},\\r\\nsponsors={Robotics and Automation Society; IEEE; Region Nord-Pas de Calais},\\r\\naddress={Paris},\\r\\nissn={10846999},\\r\\nisbn={9781467303248},\\r\\ncoden={PMEME},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Proc. IEEE Int. Conf. Micro Electro Mech. Syst. MEMS},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Sim, J.\",\"Borghi, N.\",\"Ribeiro, A.\",\"Sorokina, M.\",\"Shcherbakova, O.\",\"Ramallo, D.\",\"Dunn, A.\",\"Nelson, W.\",\"Pruitt, B.\"],\"key\":\"Sim2012854\",\"id\":\"Sim2012854\",\"bibbaseid\":\"sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&doi=10.1109%2fMEMSYS.2012.6170320&partnerID=40&md5=10bc083a2824dc630c10ffed9ff11ea6\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bruder\"],\"firstnames\":[\"J.T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Chen\"],\"firstnames\":[\"P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limbach\"],\"firstnames\":[\"K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Patterson\"],\"firstnames\":[\"N.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stefaniak\"],\"firstnames\":[\"M.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konovalova\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Thomas\"],\"firstnames\":[\"C.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hamilton\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"King\"],\"firstnames\":[\"C.R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Richie\"],\"firstnames\":[\"T.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Doolan\"],\"firstnames\":[\"D.L.\"],\"suffixes\":[]}],\"title\":\"Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses\",\"journal\":\"PLoS ONE\",\"year\":\"2012\",\"volume\":\"7\",\"number\":\"4\",\"doi\":\"10.1371/journal.pone.0033920\",\"art_number\":\"e33920\",\"note\":\"cited By 24\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&doi=10.1371%2fjournal.pone.0033920&partnerID=40&md5=615d0f3eb9de5faa02e836da1c904c89\",\"affiliation\":\"Research, GenVec, Inc., Gaithersburg, MD, United States; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States; Queensland Institute of Medical Research, Brisbane, Australia; University of California San Diego, La Jolla, CA, United States; MedImmune, Inc., Gaithersburg, MD, United States; International Aids Vaccine Initiative, New York, NY, United States\",\"abstract\":\"The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.\",\"correspondence_address1\":\"Bruder, J. T.; Research, GenVec, Inc., Gaithersburg, MD, United States; email: jbruder@genvec.com\",\"issn\":\"19326203\",\"pubmed_id\":\"22496772\",\"language\":\"English\",\"abbrev_source_title\":\"PLoS ONE\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Bruder2012,\\r\\nauthor={Bruder, J.T. and Semenova, E. and Chen, P. and Limbach, K. and Patterson, N.B. and Stefaniak, M.E. and Konovalova, S. and Thomas, C. and Hamilton, M. and King, C.R. and Richie, T.L. and Doolan, D.L.},\\r\\ntitle={Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses},\\r\\njournal={PLoS ONE},\\r\\nyear={2012},\\r\\nvolume={7},\\r\\nnumber={4},\\r\\ndoi={10.1371/journal.pone.0033920},\\r\\nart_number={e33920},\\r\\nnote={cited By 24},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&doi=10.1371%2fjournal.pone.0033920&partnerID=40&md5=615d0f3eb9de5faa02e836da1c904c89},\\r\\naffiliation={Research, GenVec, Inc., Gaithersburg, MD, United States; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States; Queensland Institute of Medical Research, Brisbane, Australia; University of California San Diego, La Jolla, CA, United States; MedImmune, Inc., Gaithersburg, MD, United States; International Aids Vaccine Initiative, New York, NY, United States},\\r\\nabstract={The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.},\\r\\ncorrespondence_address1={Bruder, J. T.; Research, GenVec, Inc., Gaithersburg, MD, United States; email: jbruder@genvec.com},\\r\\nissn={19326203},\\r\\npubmed_id={22496772},\\r\\nlanguage={English},\\r\\nabbrev_source_title={PLoS ONE},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Bruder, J.\",\"Semenova, E.\",\"Chen, P.\",\"Limbach, K.\",\"Patterson, N.\",\"Stefaniak, M.\",\"Konovalova, S.\",\"Thomas, C.\",\"Hamilton, M.\",\"King, C.\",\"Richie, T.\",\"Doolan, D.\"],\"key\":\"Bruder2012\",\"id\":\"Bruder2012\",\"bibbaseid\":\"bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&doi=10.1371%2fjournal.pone.0033920&partnerID=40&md5=615d0f3eb9de5faa02e836da1c904c89\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ilatovskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Petukhov\"],\"firstnames\":[\"M.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"SANS spectra of the fractal supernucleosomal chromatin structure models\",\"journal\":\"Journal of Physics: Conference Series\",\"year\":\"2012\",\"volume\":\"351\",\"number\":\"1\",\"doi\":\"10.1088/1742-6596/351/1/012007\",\"art_number\":\"012007\",\"note\":\"cited By 7; Conference of 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich's Birth ; Conference Date: 27 May 2011 Through 30 May 2011; Conference Code:89603\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&doi=10.1088%2f1742-6596%2f351%2f1%2f012007&partnerID=40&md5=4cb28476bafca2cc34da1a137dfb05ea\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation\",\"abstract\":\"The eukaryotic genome consists of chromatin - a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions - 3 and 2. © Published under licence by IOP Publishing Ltd.\",\"correspondence_address1\":\"Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru\",\"sponsors\":\"Comenius University in Bratislava; Horia Hulubei Natl. Inst. Phys. Nucl. Eng. (IFIN HH); Institute of Macromolecular Chemistry AS CR; Jt. Inst. Nucl. Res., Frank Lab. Neutron Phys.\",\"publisher\":\"Institute of Physics Publishing\",\"address\":\"Dubna\",\"issn\":\"17426588\",\"language\":\"English\",\"abbrev_source_title\":\"J. Phys. Conf. Ser.\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Ilatovskiy2012,\\r\\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\\r\\ntitle={SANS spectra of the fractal supernucleosomal chromatin structure models},\\r\\njournal={Journal of Physics: Conference Series},\\r\\nyear={2012},\\r\\nvolume={351},\\r\\nnumber={1},\\r\\ndoi={10.1088/1742-6596/351/1/012007},\\r\\nart_number={012007},\\r\\nnote={cited By 7; Conference of 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich's Birth ; Conference Date: 27 May 2011 Through 30 May 2011;  Conference Code:89603},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&doi=10.1088%2f1742-6596%2f351%2f1%2f012007&partnerID=40&md5=4cb28476bafca2cc34da1a137dfb05ea},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\\r\\nabstract={The eukaryotic genome consists of chromatin - a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions - 3 and 2. © Published under licence by IOP Publishing Ltd.},\\r\\ncorrespondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},\\r\\nsponsors={Comenius University in Bratislava; Horia Hulubei Natl. Inst. Phys. Nucl. Eng. (IFIN HH); Institute of Macromolecular Chemistry AS CR; Jt. Inst. Nucl. Res., Frank Lab. Neutron Phys.},\\r\\npublisher={Institute of Physics Publishing},\\r\\naddress={Dubna},\\r\\nissn={17426588},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J. Phys. Conf. Ser.},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ilatovskiy, A.\",\"Lebedev, D.\",\"Filatov, M.\",\"Petukhov, M.\",\"Isaev-Ivanov, V.\"],\"key\":\"Ilatovskiy2012\",\"id\":\"Ilatovskiy2012\",\"bibbaseid\":\"ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&doi=10.1088%2f1742-6596%2f351%2f1%2f012007&partnerID=40&md5=4cb28476bafca2cc34da1a137dfb05ea\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominsky\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanova-Smolenskaya\"],\"firstnames\":[\"I.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagyeva\"],\"firstnames\":[\"G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Illarioshkin\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborska\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson's disease\",\"journal\":\"Movement Disorders\",\"year\":\"2012\",\"volume\":\"27\",\"number\":\"1\",\"pages\":\"139-143\",\"doi\":\"10.1002/mds.23901\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&doi=10.1002%2fmds.23901&partnerID=40&md5=6dc7221fa668d60863daa976f09cf055\",\"affiliation\":\"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation; Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russian Federation\",\"abstract\":\"Background:: Deletions and duplications of single exons or exon groups account for a large proportion of the PARK2 gene mutations described in juvenile autosomal recessive Parkinson's disease (PD). Methods:: We analyzed rearrangements in exons 1 to 12 of the PARK2 gene in Russian sporadic patients with early-onset PD (EOPD) and late-onset PD (LOPD). Results:: The frequency of EOPD and LOPD patients carrying these mutations was 12.4% and 3.8%, respectively. The most frequent rearrangements were detected in exons 3 and 4. The odds ratio for EOPD in individuals carrying PARK2 exon deletions and duplications was 13.95 (95% confidence interval [CI], 1.846-105.46; P = .0022). In addition, we found a correlation between exon rearrangements in PARK2 and the age at onset of PD, presence of dystonia, and symmetrical course of the disease. Conclusions:: Exon rearrangements in the PARK2 gene play a significant role in the pathogenesis of sporadic PD in Russian patients. © 2011 Movement Disorder Society.\",\"author_keywords\":\"Deletion; Duplication; PARK2 exon rearrangements; Parkinson's disease\",\"correspondence_address1\":\"Illarioshkin, S.N.Volokolamskoe Shosse 80, Moscow 125367, Russian Federation; email: snillario@gmail.com\",\"issn\":\"08853185\",\"coden\":\"MOVDE\",\"pubmed_id\":\"21915905\",\"language\":\"English\",\"abbrev_source_title\":\"Mov. Disord.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2012139,\\r\\nauthor={Semenova, E.V. and Shadrina, M.I. and Slominsky, P.A. and Ivanova-Smolenskaya, I.A. and Bagyeva, G. and Illarioshkin, S.N. and Limborska, S.A.},\\r\\ntitle={Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson's disease},\\r\\njournal={Movement Disorders},\\r\\nyear={2012},\\r\\nvolume={27},\\r\\nnumber={1},\\r\\npages={139-143},\\r\\ndoi={10.1002/mds.23901},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&doi=10.1002%2fmds.23901&partnerID=40&md5=6dc7221fa668d60863daa976f09cf055},\\r\\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation; Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russian Federation},\\r\\nabstract={Background:: Deletions and duplications of single exons or exon groups account for a large proportion of the PARK2 gene mutations described in juvenile autosomal recessive Parkinson's disease (PD). Methods:: We analyzed rearrangements in exons 1 to 12 of the PARK2 gene in Russian sporadic patients with early-onset PD (EOPD) and late-onset PD (LOPD). Results:: The frequency of EOPD and LOPD patients carrying these mutations was 12.4% and 3.8%, respectively. The most frequent rearrangements were detected in exons 3 and 4. The odds ratio for EOPD in individuals carrying PARK2 exon deletions and duplications was 13.95 (95% confidence interval [CI], 1.846-105.46; P = .0022). In addition, we found a correlation between exon rearrangements in PARK2 and the age at onset of PD, presence of dystonia, and symmetrical course of the disease. Conclusions:: Exon rearrangements in the PARK2 gene play a significant role in the pathogenesis of sporadic PD in Russian patients. © 2011 Movement Disorder Society.},\\r\\nauthor_keywords={Deletion;  Duplication;  PARK2 exon rearrangements;  Parkinson's disease},\\r\\ncorrespondence_address1={Illarioshkin, S.N.Volokolamskoe Shosse 80, Moscow 125367, Russian Federation; email: snillario@gmail.com},\\r\\nissn={08853185},\\r\\ncoden={MOVDE},\\r\\npubmed_id={21915905},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mov. Disord.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Shadrina, M.\",\"Slominsky, P.\",\"Ivanova-Smolenskaya, I.\",\"Bagyeva, G.\",\"Illarioshkin, S.\",\"Limborska, S.\"],\"key\":\"Semenova2012139\",\"id\":\"Semenova2012139\",\"bibbaseid\":\"semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&doi=10.1002%2fmds.23901&partnerID=40&md5=6dc7221fa668d60863daa976f09cf055\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ilatovskiy\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grigoriev\"],\"firstnames\":[\"M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Petukhov\"],\"firstnames\":[\"M.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale\",\"journal\":\"Journal of Applied Physics\",\"year\":\"2011\",\"volume\":\"110\",\"number\":\"10\",\"doi\":\"10.1063/1.3661987\",\"art_number\":\"102217\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&doi=10.1063%2f1.3661987&partnerID=40&md5=8a4868aeeffdd6637dac55d1e4fa84a9\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnical University, St. Petersburg, Russian Federation; Laboratoire Biologie Moléculaire Eukaryote, CNRS-Université Paul-Sabathier, Toulouse, France\",\"abstract\":\"Eukaryotic genome is a highly compacted nucleoprotein complex organized in a hierarchical structure based on nucleosomes. Detailed organization of this structure remains unknown. In the present work we developed algorithms for geometry modeling of the supernucleosomal chromatin structure and for computing distance distribution functions and small-angle neutron scattering (SANS) spectra of the genome-scale (∼106 nucleosomes) chromatin structure at residue resolution. Our physical nucleosome model was based on the mononucleosome crystal structure. A nucleosome was assumed to be rigid within a local coordinate system. Interface parameters between nucleosomes can be set for each nucleosome independently. Pair distance distributions were computed with Monte Carlo simulation. SANS spectra were calculated with Fourier transformation of weighted distance distribution; the concentration of heavy water in solvent and probability of H/D exchange were taken into account. Two main modes of supernucleosomal structure generation were used. In a free generation mode all interface parameters were chosen randomly, whereas nucleosome self-intersections were not allowed. The second generation mode (generation in volume) enabled spherical or cubical wall restrictions. It was shown that calculated SANS spectra for a number of our models were in general agreement with available experimental data. © 2011 American Institute of Physics.\",\"correspondence_address1\":\"Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru\",\"issn\":\"00218979\",\"coden\":\"JAPIA\",\"language\":\"English\",\"abbrev_source_title\":\"J Appl Phys\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ilatovskiy2011,\\r\\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Grigoriev, M. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\\r\\ntitle={Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale},\\r\\njournal={Journal of Applied Physics},\\r\\nyear={2011},\\r\\nvolume={110},\\r\\nnumber={10},\\r\\ndoi={10.1063/1.3661987},\\r\\nart_number={102217},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&doi=10.1063%2f1.3661987&partnerID=40&md5=8a4868aeeffdd6637dac55d1e4fa84a9},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnical University, St. Petersburg, Russian Federation; Laboratoire Biologie Moléculaire Eukaryote, CNRS-Université Paul-Sabathier, Toulouse, France},\\r\\nabstract={Eukaryotic genome is a highly compacted nucleoprotein complex organized in a hierarchical structure based on nucleosomes. Detailed organization of this structure remains unknown. In the present work we developed algorithms for geometry modeling of the supernucleosomal chromatin structure and for computing distance distribution functions and small-angle neutron scattering (SANS) spectra of the genome-scale (∼106 nucleosomes) chromatin structure at residue resolution. Our physical nucleosome model was based on the mononucleosome crystal structure. A nucleosome was assumed to be rigid within a local coordinate system. Interface parameters between nucleosomes can be set for each nucleosome independently. Pair distance distributions were computed with Monte Carlo simulation. SANS spectra were calculated with Fourier transformation of weighted distance distribution; the concentration of heavy water in solvent and probability of H/D exchange were taken into account. Two main modes of supernucleosomal structure generation were used. In a free generation mode all interface parameters were chosen randomly, whereas nucleosome self-intersections were not allowed. The second generation mode (generation in volume) enabled spherical or cubical wall restrictions. It was shown that calculated SANS spectra for a number of our models were in general agreement with available experimental data. © 2011 American Institute of Physics.},\\r\\ncorrespondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},\\r\\nissn={00218979},\\r\\ncoden={JAPIA},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J Appl Phys},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ilatovskiy, A.\",\"Lebedev, D.\",\"Filatov, M.\",\"Grigoriev, M.\",\"Petukhov, M.\",\"Isaev-Ivanov, V.\"],\"key\":\"Ilatovskiy2011\",\"id\":\"Ilatovskiy2011\",\"bibbaseid\":\"ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&doi=10.1063%2f1.3661987&partnerID=40&md5=8a4868aeeffdd6637dac55d1e4fa84a9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Study of chromatin decondensation factors in human spermatozoids by flow cytometry\",\"journal\":\"Russian Journal of Developmental Biology\",\"year\":\"2011\",\"volume\":\"42\",\"number\":\"1\",\"pages\":\"16-24\",\"doi\":\"10.1134/S1062360411010097\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&doi=10.1134%2fS1062360411010097&partnerID=40&md5=925eb3cf7929f0e3f073ac255b316768\",\"affiliation\":\"Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation\",\"abstract\":\"To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm. © 2011 Pleiades Publishing, Ltd.\",\"author_keywords\":\"endogenous nuclear proteases; fertilization; flow cytometry; human spermatozoids; protease inhibitors\",\"correspondence_address1\":\"Filatov, M. V.; Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"10623604\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Dev. Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova201116,\\r\\nauthor={Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Study of chromatin decondensation factors in human spermatozoids by flow cytometry},\\r\\njournal={Russian Journal of Developmental Biology},\\r\\nyear={2011},\\r\\nvolume={42},\\r\\nnumber={1},\\r\\npages={16-24},\\r\\ndoi={10.1134/S1062360411010097},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&doi=10.1134%2fS1062360411010097&partnerID=40&md5=925eb3cf7929f0e3f073ac255b316768},\\r\\naffiliation={Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation},\\r\\nabstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm. © 2011 Pleiades Publishing, Ltd.},\\r\\nauthor_keywords={endogenous nuclear proteases;  fertilization;  flow cytometry;  human spermatozoids;  protease inhibitors},\\r\\ncorrespondence_address1={Filatov, M. V.; Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={10623604},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Dev. Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Semenova201116\",\"id\":\"Semenova201116\",\"bibbaseid\":\"semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&doi=10.1134%2fS1062360411010097&partnerID=40&md5=925eb3cf7929f0e3f073ac255b316768\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"[Study of chromatin decondensation factors in human spermatozoids by flow cytometry].\",\"journal\":\"Ontogenez\",\"year\":\"2011\",\"volume\":\"42\",\"number\":\"1\",\"pages\":\"20-29\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&partnerID=40&md5=e4b4997f53d5d4069bc4d51f710afbb4\",\"abstract\":\"To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm.\",\"correspondence_address1\":\"Semenova, E.V.\",\"issn\":\"04751450\",\"pubmed_id\":\"21442899\",\"language\":\"Russian\",\"abbrev_source_title\":\"Ontogenez\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova201120,\\r\\nauthor={Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={[Study of chromatin decondensation factors in human spermatozoids by flow cytometry].},\\r\\njournal={Ontogenez},\\r\\nyear={2011},\\r\\nvolume={42},\\r\\nnumber={1},\\r\\npages={20-29},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&partnerID=40&md5=e4b4997f53d5d4069bc4d51f710afbb4},\\r\\nabstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm.},\\r\\ncorrespondence_address1={Semenova, E.V.},\\r\\nissn={04751450},\\r\\npubmed_id={21442899},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Ontogenez},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Semenova201120\",\"id\":\"Semenova201120\",\"bibbaseid\":\"semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&partnerID=40&md5=e4b4997f53d5d4069bc4d51f710afbb4\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Garmai\"],\"firstnames\":[\"Yu.P.\"],\"suffixes\":[]}],\"title\":\"Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo\",\"journal\":\"Klinichescheskaya Laboratornaya Diagnostika\",\"year\":\"2010\",\"number\":\"12\",\"pages\":\"35-43\",\"note\":\"cited By 8\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&partnerID=40&md5=c3b287531eda58217f247310540fc220\",\"abstract\":\"Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection ofexosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked.\",\"author_keywords\":\"Dynamic light scattering technique; Exosomes; Fibroblasts; Lymphocytes\",\"correspondence_address1\":\"Filatov, M. V.email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"08692084\",\"pubmed_id\":\"21395053\",\"language\":\"Russian\",\"abbrev_source_title\":\"Klin. Lab. Diagn.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov201035,\\r\\nauthor={Filatov, M.V. and Landa, S.B. and Pantina, R.A. and Garmai, Yu.P.},\\r\\ntitle={Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo},\\r\\njournal={Klinichescheskaya Laboratornaya Diagnostika},\\r\\nyear={2010},\\r\\nnumber={12},\\r\\npages={35-43},\\r\\nnote={cited By 8},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&partnerID=40&md5=c3b287531eda58217f247310540fc220},\\r\\nabstract={Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection ofexosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked.},\\r\\nauthor_keywords={Dynamic light scattering technique;  Exosomes;  Fibroblasts;  Lymphocytes},\\r\\ncorrespondence_address1={Filatov, M. V.email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={08692084},\\r\\npubmed_id={21395053},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Klin. Lab. Diagn.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Landa, S.\",\"Pantina, R.\",\"Garmai, Y.\"],\"key\":\"Filatov201035\",\"id\":\"Filatov201035\",\"bibbaseid\":\"filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&partnerID=40&md5=c3b287531eda58217f247310540fc220\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanov\"],\"firstnames\":[\"E.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Detection of inflammatory processes during various diseases by the method of flow cytofluorometry\",\"journal\":\"Bulletin of Experimental Biology and Medicine\",\"year\":\"2010\",\"volume\":\"149\",\"number\":\"4\",\"pages\":\"485-489\",\"doi\":\"10.1007/s10517-010-0976-2\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&doi=10.1007%2fs10517-010-0976-2&partnerID=40&md5=283fbb036945227dc1e8fc31f3e078a3\",\"affiliation\":\"St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"abstract\":\"Oxidative (respiratory) burst is an important manifestation of inflammation. Precise quantitative assessment of this reaction by flow cytometry made it possible to record and evaluate the severity of the inflammatory processes in a wide spectrum of diseases including diphtheria, hepatitis, pneumonia, bronchial asthma, arthritis, vasculitis, postoperative complications, tuberculosis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and myocardial infarction. This approach can be employed as a highly sensitive method of detection of inflammatory reactions and monitoring of their course in various pathological processes. © 2010 Springer Science+Business Media, Inc.\",\"author_keywords\":\"flow cytometry; inflammation; respiratory burst\",\"correspondence_address1\":\"Varfolomeeva, E. Y.; St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation; email: e_varf@mail.ru\",\"issn\":\"00074888\",\"coden\":\"BEXBA\",\"pubmed_id\":\"21234449\",\"language\":\"English\",\"abbrev_source_title\":\"Bull. Exp. Biol. Med.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Varfolomeeva2010485,\\r\\nauthor={Varfolomeeva, E.Y. and Ivanov, E.I. and Drobchenko, E.A. and Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Detection of inflammatory processes during various diseases by the method of flow cytofluorometry},\\r\\njournal={Bulletin of Experimental Biology and Medicine},\\r\\nyear={2010},\\r\\nvolume={149},\\r\\nnumber={4},\\r\\npages={485-489},\\r\\ndoi={10.1007/s10517-010-0976-2},\\r\\nnote={cited By 3},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&doi=10.1007%2fs10517-010-0976-2&partnerID=40&md5=283fbb036945227dc1e8fc31f3e078a3},\\r\\naffiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\nabstract={Oxidative (respiratory) burst is an important manifestation of inflammation. Precise quantitative assessment of this reaction by flow cytometry made it possible to record and evaluate the severity of the inflammatory processes in a wide spectrum of diseases including diphtheria, hepatitis, pneumonia, bronchial asthma, arthritis, vasculitis, postoperative complications, tuberculosis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and myocardial infarction. This approach can be employed as a highly sensitive method of detection of inflammatory reactions and monitoring of their course in various pathological processes. © 2010 Springer Science+Business Media, Inc.},\\r\\nauthor_keywords={flow cytometry;  inflammation;  respiratory burst},\\r\\ncorrespondence_address1={Varfolomeeva, E. Y.; St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation; email: e_varf@mail.ru},\\r\\nissn={00074888},\\r\\ncoden={BEXBA},\\r\\npubmed_id={21234449},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Bull. Exp. Biol. Med.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Varfolomeeva, E.\",\"Ivanov, E.\",\"Drobchenko, E.\",\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Varfolomeeva2010485\",\"id\":\"Varfolomeeva2010485\",\"bibbaseid\":\"varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&doi=10.1007%2fs10517-010-0976-2&partnerID=40&md5=283fbb036945227dc1e8fc31f3e078a3\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Alexeyev\"],\"firstnames\":[\"V.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kaboev\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Scherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Immunological similarity of diphtheria toxin and EGF receptor\",\"journal\":\"Tsitologiya\",\"year\":\"2010\",\"volume\":\"52\",\"number\":\"5\",\"pages\":\"364-370\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&partnerID=40&md5=8a292ba5a5e9c827cff8d9e690c24c38\",\"affiliation\":\"Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States; Molecular and Radiation Biophysics Division of St. Petersburg, Nuclear Physics Institute, RAS, St. Petersburg, Gatchina, Russian Federation; Department of Molecular and Cellular Physiology, Stanford University, Stanford, United States\",\"abstract\":\"Cardiomyopathy and neuropathy are the two commonly observed complications in diphtheria patients and in, some instances, individuals vaccinated against diphtheria. The nature of these complications remains not well understood. It was suggested that autoimmunity may play a role in the development of these afflictions. Based on functional similarities between diphtheria toxin (DT) and epidermal growth factor receptor (EGFR), which both can bind to the heparin-binding EGF-like growth factor (HB-EGF) precursors, we suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized by antibodies raised against EGFR and by serum antibodies from diphtheria patients. Moreover serum of diphtheria patients competitively inhibits binding of anti-EGFR antibodies to the receptor. The truncated diphtheria toxin without B-subunit could be detected by serum antibodies of diphtheria patients, but not by anti-EGFR antibodies. Collectively, these studies demonstrate cross-reactivity of antibodies raised against B-subunit of DT and extracellular domain of EGFR and suggest that clinically observed post-diphtheria complications may result from autoimmune inhibition of EGFR function and possible destruction of receptor-positive tissues.\",\"author_keywords\":\"Cross-immunoreactivity; Diphtheria toxin; Epidermal growth factor receptor; Post-diphtheria complications\",\"correspondence_address1\":\"Alexeyev, V. Yu.; Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"20586270\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Alexeyev2010364,\\r\\nauthor={Alexeyev, V.Yu. and Kaboev, O.K. and Scherbakova, O.G. and Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Immunological similarity of diphtheria toxin and EGF receptor},\\r\\njournal={Tsitologiya},\\r\\nyear={2010},\\r\\nvolume={52},\\r\\nnumber={5},\\r\\npages={364-370},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&partnerID=40&md5=8a292ba5a5e9c827cff8d9e690c24c38},\\r\\naffiliation={Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States; Molecular and Radiation Biophysics Division of St. Petersburg, Nuclear Physics Institute, RAS, St. Petersburg, Gatchina, Russian Federation; Department of Molecular and Cellular Physiology, Stanford University, Stanford, United States},\\r\\nabstract={Cardiomyopathy and neuropathy are the two commonly observed complications in diphtheria patients and in, some instances, individuals vaccinated against diphtheria. The nature of these complications remains not well understood. It was suggested that autoimmunity may play a role in the development of these afflictions. Based on functional similarities between diphtheria toxin (DT) and epidermal growth factor receptor (EGFR), which both can bind to the heparin-binding EGF-like growth factor (HB-EGF) precursors, we suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized by antibodies raised against EGFR and by serum antibodies from diphtheria patients. Moreover serum of diphtheria patients competitively inhibits binding of anti-EGFR antibodies to the receptor. The truncated diphtheria toxin without B-subunit could be detected by serum antibodies of diphtheria patients, but not by anti-EGFR antibodies. Collectively, these studies demonstrate cross-reactivity of antibodies raised against B-subunit of DT and extracellular domain of EGFR and suggest that clinically observed post-diphtheria complications may result from autoimmune inhibition of EGFR function and possible destruction of receptor-positive tissues.},\\r\\nauthor_keywords={Cross-immunoreactivity;  Diphtheria toxin;  Epidermal growth factor receptor;  Post-diphtheria complications},\\r\\ncorrespondence_address1={Alexeyev, V. Yu.; Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={20586270},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Alexeyev, V.\",\"Kaboev, O.\",\"Scherbakova, O.\",\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Alexeyev2010364\",\"id\":\"Alexeyev2010364\",\"bibbaseid\":\"alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&partnerID=40&md5=8a292ba5a5e9c827cff8d9e690c24c38\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lauter\"],\"firstnames\":[\"H.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kuklin\"],\"firstnames\":[\"A.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Islamov\"],\"firstnames\":[\"A.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering\",\"journal\":\"Physics of the Solid State\",\"year\":\"2010\",\"volume\":\"52\",\"number\":\"5\",\"pages\":\"1063-1073\",\"doi\":\"10.1134/S1063783410050379\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&doi=10.1134%2fS1063783410050379&partnerID=40&md5=b7266c4aa86093bcd4dc258952790714\",\"affiliation\":\"Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; Research and Educational Center Biophysics of the St. Petersburg State Polytechnical University, Konstantinov Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Institut Laue-Langevin, BP 156, Grenoble 38042, France; Joint Institute for Nuclear Research, ul. Joliot-Curie 6, Dubna, Moscow oblast 141980, Russian Federation\",\"abstract\":\"The nucleosome structure in native nuclei of normal (chicken erythrocyte and rat leukocyte nuclei) and anomalously proliferating (the human cervical adenocarcinoma cell line HeLa and the Chinese hamster fibroblast cell line A238) cells has been investigated using small-angle neutron scattering. The experimental results obtained allow one to make the inference that the parameters of the nucleosome structure for the chicken erythrocyte and rat leukocyte nuclei (on average over the nucleus) are close to the universally accepted values and that the distance distribution function is bimodal. The bimodality of the distance distribution function reflects a narrow distribution of distances between nucleosomes (on average over the nucleus) at the fibril level of the chromatin organization. The histone core of the nucleosome structure in the nuclei of the HeLa and A238 cells (on average over the nucleus) is considerably less compact than that in the chicken erythrocyte and rat leukocyte nuclei, and the distance distribution function does not exhibit indications of the bimodality. © 2010 Pleiades Publishing, Ltd.\",\"correspondence_address1\":\"Filatov, M. V.; Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"10637834\",\"language\":\"English\",\"abbrev_source_title\":\"Phys. Solid State\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Isaev-Ivanov20101063,\\r\\nauthor={Isaev-Ivanov, V.V. and Lebedev, D.V. and Lauter, H. and Pantina, R.A. and Kuklin, A.I. and Islamov, A.K. and Filatov, M.V.},\\r\\ntitle={Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering},\\r\\njournal={Physics of the Solid State},\\r\\nyear={2010},\\r\\nvolume={52},\\r\\nnumber={5},\\r\\npages={1063-1073},\\r\\ndoi={10.1134/S1063783410050379},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&doi=10.1134%2fS1063783410050379&partnerID=40&md5=b7266c4aa86093bcd4dc258952790714},\\r\\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; Research and Educational Center Biophysics of the St. Petersburg State Polytechnical University, Konstantinov Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Institut Laue-Langevin, BP 156, Grenoble 38042, France; Joint Institute for Nuclear Research, ul. Joliot-Curie 6, Dubna, Moscow oblast 141980, Russian Federation},\\r\\nabstract={The nucleosome structure in native nuclei of normal (chicken erythrocyte and rat leukocyte nuclei) and anomalously proliferating (the human cervical adenocarcinoma cell line HeLa and the Chinese hamster fibroblast cell line A238) cells has been investigated using small-angle neutron scattering. The experimental results obtained allow one to make the inference that the parameters of the nucleosome structure for the chicken erythrocyte and rat leukocyte nuclei (on average over the nucleus) are close to the universally accepted values and that the distance distribution function is bimodal. The bimodality of the distance distribution function reflects a narrow distribution of distances between nucleosomes (on average over the nucleus) at the fibril level of the chromatin organization. The histone core of the nucleosome structure in the nuclei of the HeLa and A238 cells (on average over the nucleus) is considerably less compact than that in the chicken erythrocyte and rat leukocyte nuclei, and the distance distribution function does not exhibit indications of the bimodality. © 2010 Pleiades Publishing, Ltd.},\\r\\ncorrespondence_address1={Filatov, M. V.; Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={10637834},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Phys. Solid State},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Isaev-Ivanov, V.\",\"Lebedev, D.\",\"Lauter, H.\",\"Pantina, R.\",\"Kuklin, A.\",\"Islamov, A.\",\"Filatov, M.\"],\"key\":\"Isaev-Ivanov20101063\",\"id\":\"Isaev-Ivanov20101063\",\"bibbaseid\":\"isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&doi=10.1134%2fS1063783410050379&partnerID=40&md5=b7266c4aa86093bcd4dc258952790714\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Angelotti\"],\"firstnames\":[\"T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Daunt\"],\"firstnames\":[\"D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kobilka\"],\"firstnames\":[\"B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hurt\"],\"firstnames\":[\"C.M.\"],\"suffixes\":[]}],\"title\":\"Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal\",\"journal\":\"Traffic\",\"year\":\"2010\",\"volume\":\"11\",\"number\":\"4\",\"pages\":\"560-578\",\"doi\":\"10.1111/j.1600-0854.2010.01033.x\",\"note\":\"cited By 29\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&doi=10.1111%2fj.1600-0854.2010.01033.x&partnerID=40&md5=58b7e08a45bab6703d592151df3ebfcd\",\"affiliation\":\"Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 259, Campus Drive, Stanford, CA 94035, United States\",\"abstract\":\"Plasma membrane (PM) expression of G-protein coupled receptors (GPCRs) is required for activation by extracellular ligands; however, mechanisms that regulate PM expression of GPCRs are poorly understood. For some GPCRs, such as alpha2c-adrenergic receptors (α2c-ARs), heterologous expression in non-native cells results in limited PM expression and extensive endoplasmic reticulum (ER) retention. Recently, ER export/retentions signals have been proposed to regulate cellular trafficking of several GPCRs. By utilizing a chimeric α2a/α2c-AR strategy, we identified an evolutionary conserved hydrophobic sequence (ALAAALAAAAA) in the extracellular amino terminal region that is responsible in part for α2c-AR subtype-specific trafficking. To our knowledge, this is the first luminal ER retention signal reported for a GPCR. Removal or disruption of the ER retention signal dramatically increased PM expression and decreased ER retention. Conversely, transplantation of this hydrophobic sequence into α2a-ARs reduced their PM expression and increased ER retention. This evolutionary conserved hydrophobic trafficking signal within α2c-ARs serves as a regulator of GPCR trafficking. © 2010 John Wiley &amp; Sons A/S.\",\"author_keywords\":\"ER export; ER retention; GPCR trafficking; Protein sorting; Retention signal\",\"correspondence_address1\":\"Angelotti, T.; Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; email: timangel@stanford.edu\",\"issn\":\"13989219\",\"coden\":\"TRAFF\",\"pubmed_id\":\"20059747\",\"language\":\"English\",\"abbrev_source_title\":\"Traffic\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Angelotti2010560,\\r\\nauthor={Angelotti, T. and Daunt, D. and Shcherbakova, O.G. and Kobilka, B. and Hurt, C.M.},\\r\\ntitle={Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal},\\r\\njournal={Traffic},\\r\\nyear={2010},\\r\\nvolume={11},\\r\\nnumber={4},\\r\\npages={560-578},\\r\\ndoi={10.1111/j.1600-0854.2010.01033.x},\\r\\nnote={cited By 29},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&doi=10.1111%2fj.1600-0854.2010.01033.x&partnerID=40&md5=58b7e08a45bab6703d592151df3ebfcd},\\r\\naffiliation={Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 259, Campus Drive, Stanford, CA 94035, United States},\\r\\nabstract={Plasma membrane (PM) expression of G-protein coupled receptors (GPCRs) is required for activation by extracellular ligands; however, mechanisms that regulate PM expression of GPCRs are poorly understood. For some GPCRs, such as alpha2c-adrenergic receptors (α2c-ARs), heterologous expression in non-native cells results in limited PM expression and extensive endoplasmic reticulum (ER) retention. Recently, ER export/retentions signals have been proposed to regulate cellular trafficking of several GPCRs. By utilizing a chimeric α2a/α2c-AR strategy, we identified an evolutionary conserved hydrophobic sequence (ALAAALAAAAA) in the extracellular amino terminal region that is responsible in part for α2c-AR subtype-specific trafficking. To our knowledge, this is the first luminal ER retention signal reported for a GPCR. Removal or disruption of the ER retention signal dramatically increased PM expression and decreased ER retention. Conversely, transplantation of this hydrophobic sequence into α2a-ARs reduced their PM expression and increased ER retention. This evolutionary conserved hydrophobic trafficking signal within α2c-ARs serves as a regulator of GPCR trafficking. © 2010 John Wiley &amp; Sons A/S.},\\r\\nauthor_keywords={ER export;  ER retention;  GPCR trafficking;  Protein sorting;  Retention signal},\\r\\ncorrespondence_address1={Angelotti, T.; Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; email: timangel@stanford.edu},\\r\\nissn={13989219},\\r\\ncoden={TRAFF},\\r\\npubmed_id={20059747},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Traffic},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Angelotti, T.\",\"Daunt, D.\",\"Shcherbakova, O.\",\"Kobilka, B.\",\"Hurt, C.\"],\"key\":\"Angelotti2010560\",\"id\":\"Angelotti2010560\",\"bibbaseid\":\"angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&doi=10.1111%2fj.1600-0854.2010.01033.x&partnerID=40&md5=58b7e08a45bab6703d592151df3ebfcd\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1\",\"journal\":\"Ėksperimental'naia i klinicheskaia gastroėnterologiia = Experimental & clinical gastroenterology\",\"year\":\"2009\",\"number\":\"6\",\"pages\":\"138-142\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&partnerID=40&md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9\",\"abstract\":\"The purpose of the research has been the study of possibilities for the pharmacological correction of the morphological changes in liver and lipid metabolism disorders in experimental type 1 diabetes. Simvastatin, new compound from the group of antioxidants–3-hydroxipyridine derivate under the code HP-5, and also the combination of simvastatin and HP-5 have been used for the pharmacological correction in this research. It was revealed that the using of HP-5 and the combination of simvastatin and HP-5 attenuate the order of expression of diabetic hepatopathia as the result of the histological study and hinder from the development of dislipidemia in rates with type 1 diabetes which is more expressed in comparison with the monotherapy by simvastatin. These changes also are followed with the diminishing of the free radical lipid oxidation activity by the specific antioxidant effect of the investigated 3-hydroxipyridine derivate.\",\"correspondence_address1\":\"Semenova, E.V.\",\"issn\":\"16828658\",\"pubmed_id\":\"20201297\",\"language\":\"Russian\",\"abbrev_source_title\":\"Eksp Klin Gastroenterol\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2009138,\\r\\nauthor={Semenova, E.V.},\\r\\ntitle={Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1},\\r\\njournal={Ėksperimental'naia i klinicheskaia gastroėnterologiia = Experimental & clinical gastroenterology},\\r\\nyear={2009},\\r\\nnumber={6},\\r\\npages={138-142},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&partnerID=40&md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9},\\r\\nabstract={The purpose of the research has been the study of possibilities for the pharmacological correction of the morphological changes in liver and lipid metabolism disorders in experimental type 1 diabetes. Simvastatin, new compound from the group of antioxidants--3-hydroxipyridine derivate under the code HP-5, and also the combination of simvastatin and HP-5 have been used for the pharmacological correction in this research. It was revealed that the using of HP-5 and the combination of simvastatin and HP-5 attenuate the order of expression of diabetic hepatopathia as the result of the histological study and hinder from the development of dislipidemia in rates with type 1 diabetes which is more expressed in comparison with the monotherapy by simvastatin. These changes also are followed with the diminishing of the free radical lipid oxidation activity by the specific antioxidant effect of the investigated 3-hydroxipyridine derivate.},\\r\\ncorrespondence_address1={Semenova, E.V.},\\r\\nissn={16828658},\\r\\npubmed_id={20201297},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Eksp Klin Gastroenterol},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\"],\"key\":\"Semenova2009138\",\"id\":\"Semenova2009138\",\"bibbaseid\":\"semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&partnerID=40&md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Galich\"],\"firstnames\":[\"N.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment\",\"journal\":\"Proceedings of SPIE - The International Society for Optical Engineering\",\"year\":\"2009\",\"volume\":\"7377\",\"doi\":\"10.1117/12.836452\",\"art_number\":\"73770C\",\"note\":\"cited By 2; Conference of 12th International Workshop on Nanodesign Technology and Computer Simulations ; Conference Date: 23 June 2008 Through 27 June 2008; Conference Code:77311\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&doi=10.1117%2f12.836452&partnerID=40&md5=f3819171ad8b578019e72414bc891193\",\"affiliation\":\"Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; Department of Cell Biology, B.P.Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 183300, Russian Federation\",\"abstract\":\"Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status. © 2009 SPIE.\",\"author_keywords\":\"Attractor; Bifurcation; Blood; Cell; Cytometry; Distribution; DNA; Immunofluorescence; Neutrophils; Oxidants\",\"correspondence_address1\":\"Galich, N. E.; Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; email: n.galich@mail.ru\",\"sponsors\":\"Motorola Company; Research and Production Corporation Integral; Scanwest Company; Silvaco Data Systems\",\"address\":\"Minsk\",\"issn\":\"0277786X\",\"isbn\":\"9780819476531\",\"coden\":\"PSISD\",\"language\":\"English\",\"abbrev_source_title\":\"Proc SPIE Int Soc Opt Eng\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Galich2009,\\r\\nauthor={Galich, N.E. and Filatov, M.V.},\\r\\ntitle={Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment},\\r\\njournal={Proceedings of SPIE - The International Society for Optical Engineering},\\r\\nyear={2009},\\r\\nvolume={7377},\\r\\ndoi={10.1117/12.836452},\\r\\nart_number={73770C},\\r\\nnote={cited By 2; Conference of 12th International Workshop on Nanodesign Technology and Computer Simulations ; Conference Date: 23 June 2008 Through 27 June 2008;  Conference Code:77311},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&doi=10.1117%2f12.836452&partnerID=40&md5=f3819171ad8b578019e72414bc891193},\\r\\naffiliation={Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; Department of Cell Biology, B.P.Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 183300, Russian Federation},\\r\\nabstract={Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status. © 2009 SPIE.},\\r\\nauthor_keywords={Attractor;  Bifurcation;  Blood;  Cell;  Cytometry;  Distribution;  DNA;  Immunofluorescence;  Neutrophils;  Oxidants},\\r\\ncorrespondence_address1={Galich, N. E.; Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; email: n.galich@mail.ru},\\r\\nsponsors={Motorola Company; Research and Production Corporation Integral; Scanwest Company; Silvaco Data Systems},\\r\\naddress={Minsk},\\r\\nissn={0277786X},\\r\\nisbn={9780819476531},\\r\\ncoden={PSISD},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Proc SPIE Int Soc Opt Eng},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Galich, N.\",\"Filatov, M.\"],\"key\":\"Galich2009\",\"id\":\"Galich2009\",\"bibbaseid\":\"galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&doi=10.1117%2f12.836452&partnerID=40&md5=f3819171ad8b578019e72414bc891193\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominskiǐ\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Illarioshkin\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagyeva\"],\"firstnames\":[\"G.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Karabanov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanova-Smolenskaia\"],\"firstnames\":[\"I.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborskaia\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease\",\"journal\":\"Genetika\",\"year\":\"2009\",\"volume\":\"45\",\"number\":\"4\",\"pages\":\"573-576\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&partnerID=40&md5=f39cf36c04fe528f017aa5d1c25c72f9\",\"abstract\":\"Fifty-two patients that had ParkinsonTs disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4–6 of alpha-synuclein gene using real-time PCR with Taq-Man probes. No mutations involving the examined exons dosage were revealed in alpha-synuclein gene. Thus, mutations modifying copy number of alpha-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of ParkinsonTs disease in patients from Russia.\",\"correspondence_address1\":\"Semenova, E.V.\",\"issn\":\"00166758\",\"pubmed_id\":\"19507712\",\"language\":\"Russian\",\"abbrev_source_title\":\"Genetika\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2009573,\\r\\nauthor={Semenova, E.V. and Shadrina, M.I. and Slominskiǐ, P.A. and Illarioshkin, S.N. and Bagyeva, G.K. and Karabanov, A.V. and Ivanova-Smolenskaia, I.A. and Limborskaia, S.A.},\\r\\ntitle={Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease},\\r\\njournal={Genetika},\\r\\nyear={2009},\\r\\nvolume={45},\\r\\nnumber={4},\\r\\npages={573-576},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&partnerID=40&md5=f39cf36c04fe528f017aa5d1c25c72f9},\\r\\nabstract={Fifty-two patients that had ParkinsonTs disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4--6 of alpha-synuclein gene using real-time PCR with Taq-Man probes. No mutations involving the examined exons dosage were revealed in alpha-synuclein gene. Thus, mutations modifying copy number of alpha-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of ParkinsonTs disease in patients from Russia.},\\r\\ncorrespondence_address1={Semenova, E.V.},\\r\\nissn={00166758},\\r\\npubmed_id={19507712},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Genetika},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Shadrina, M.\",\"Slominskiǐ, P.\",\"Illarioshkin, S.\",\"Bagyeva, G.\",\"Karabanov, A.\",\"Ivanova-Smolenskaia, I.\",\"Limborskaia, S.\"],\"key\":\"Semenova2009573\",\"id\":\"Semenova2009573\",\"bibbaseid\":\"semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&partnerID=40&md5=f39cf36c04fe528f017aa5d1c25c72f9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominsky\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Illarioshkin\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagyeva\"],\"firstnames\":[\"G.Kh.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Karabanov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanova-Smolenskaia\"],\"firstnames\":[\"I.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborska\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Analysis of the α-synuclein gene dosage variation associated with autosomal dominant form of Parkinson's disease\",\"journal\":\"Russian Journal of Genetics\",\"year\":\"2009\",\"volume\":\"45\",\"number\":\"4\",\"pages\":\"504-506\",\"doi\":\"10.1134/S1022795409040176\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&doi=10.1134%2fS1022795409040176&partnerID=40&md5=317f7a9d80285dad4ca92bc42d619bdb\",\"affiliation\":\"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russian Federation; Neurological Research Center, Russian Academy of Medical Sciences, Moscow, 123367, Russian Federation\",\"abstract\":\"Fifty-two patients that had Parkinson's disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4-6 of α-synuclein gene using real-time PCR with TaqMan probes. No mutations involving the examined exons dosage were revealed in α-synuclein gene. Thus, mutations modifying copy number of α-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of Parkinson's disease in patients from Russia. © 2009 Pleiades Publishing, Ltd.\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)06-04-49368-a, 07-04-01046\",\"key\":\"Semenova2009504\",\"id\":\"Semenova2009504\",\"bibbaseid\":\"anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&doi=10.1134%2fS1022795409040176&partnerID=40&md5=317f7a9d80285dad4ca92bc42d619bdb\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival\",\"journal\":\"Tsitologiya\",\"year\":\"2008\",\"volume\":\"50\",\"number\":\"11\",\"pages\":\"958-963\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&partnerID=40&md5=5313418d75141a24ef0926ff2a58d2ff\",\"affiliation\":\"Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation\",\"abstract\":\"The RAD 51 protein, an eukaryotic homologue of Escherichia coli Rec A, plays a central role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of Rad5l gene by specific small interfering (si) RNA induces cell death of the most part of investigated cancer cell lines and normal fibroblasts. Disruption of the Rad51 gene in these cells results in S or(and) G 2 cell cycle arrest leading to apoptosis. But some human cancer cell lines demonstrate abolishment of premitotic checkpoint and are not sensitive to siRNA silencing of RAD51 recombinase. Recent experiments show that normal functioning of the recombination repair system is essential for maintenance of genome stability, proliferation of vertebrate cells and, finally, for prevention of dramatic cell death.\",\"author_keywords\":\"Cell cycle; DNA reparation; Homologous recombination; Rad51\",\"correspondence_address1\":\"Shtam, T. A.; Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"19140342\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2008958,\\r\\nauthor={Shtam, T.A. and Varfolomeeva, E.Y. and Semenova, E.V. and Filatov, M.V.},\\r\\ntitle={Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival},\\r\\njournal={Tsitologiya},\\r\\nyear={2008},\\r\\nvolume={50},\\r\\nnumber={11},\\r\\npages={958-963},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&partnerID=40&md5=5313418d75141a24ef0926ff2a58d2ff},\\r\\naffiliation={Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},\\r\\nabstract={The RAD 51 protein, an eukaryotic homologue of Escherichia coli Rec A, plays a central role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of Rad5l gene by specific small interfering (si) RNA induces cell death of the most part of investigated cancer cell lines and normal fibroblasts. Disruption of the Rad51 gene in these cells results in S or(and) G 2 cell cycle arrest leading to apoptosis. But some human cancer cell lines demonstrate abolishment of premitotic checkpoint and are not sensitive to siRNA silencing of RAD51 recombinase. Recent experiments show that normal functioning of the recombination repair system is essential for maintenance of genome stability, proliferation of vertebrate cells and, finally, for prevention of dramatic cell death.},\\r\\nauthor_keywords={Cell cycle;  DNA reparation;  Homologous recombination;  Rad51},\\r\\ncorrespondence_address1={Shtam, T. A.; Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={19140342},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Varfolomeeva, E.\",\"Semenova, E.\",\"Filatov, M.\"],\"key\":\"Shtam2008958\",\"id\":\"Shtam2008958\",\"bibbaseid\":\"shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&partnerID=40&md5=5313418d75141a24ef0926ff2a58d2ff\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Role of human RAD51 recombinase in the cycle checkpoint and survival of a cell\",\"journal\":\"Cell and Tissue Biology\",\"year\":\"2008\",\"volume\":\"2\",\"number\":\"5\",\"pages\":\"463-467\",\"doi\":\"10.1134/S1990519X08050027\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&doi=10.1134%2fS1990519X08050027&partnerID=40&md5=30d4ecc52e19dc81c11a7ab184a77380\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Russian Federation\",\"abstract\":\"The RAD 51 protein, a eukaryotic homologue of Escherichia coli RecA, plays a significant role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of the Rad51 gene by specific small interfering RNA (siRNA) that inhibits cell proliferation and reduces the viability of most cells. Cells with suppressed expression of Rad51 gene have altered cell cycles and accumulate in the S and G2 phases. Our findings show that the disruption of homologous recombination leads to cell death. However, some cells, e.g., MCF-7 cells, are insensitive to the suppression of Rad51 gene expression. © MAIK Nauka 2008.\",\"author_keywords\":\"Cell cycle; DNA repair; Mologous recombination; RAD51\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)05-04-48902\",\"key\":\"Shtam2008463\",\"id\":\"Shtam2008463\",\"bibbaseid\":\"anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&doi=10.1134%2fS1990519X08050027&partnerID=40&md5=30d4ecc52e19dc81c11a7ab184a77380\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Landa\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Arutyunyan\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeyeva\"],\"firstnames\":[\"Ye.V.\"],\"suffixes\":[]}],\"title\":\"Study of plasma megamolecular complexation by laser correlation spectroscopy\",\"journal\":\"Klinichescheskaya Laboratornaya Diagnostika\",\"year\":\"2008\",\"number\":\"4\",\"pages\":\"37-41\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&partnerID=40&md5=a30b0b5ac1d900684a623822c2fcc6b0\",\"issn\":\"08692084\",\"pubmed_id\":\"18619204\",\"language\":\"Russian\",\"abbrev_source_title\":\"Klin. Lab. Diagn.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Landa200837,\\r\\nauthor={Landa, S.B. and Filatov, M.V. and Arutyunyan, A.V. and Varfolomeyeva, Ye.V.},\\r\\ntitle={Study of plasma megamolecular complexation by laser correlation spectroscopy},\\r\\njournal={Klinichescheskaya Laboratornaya Diagnostika},\\r\\nyear={2008},\\r\\nnumber={4},\\r\\npages={37-41},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&partnerID=40&md5=a30b0b5ac1d900684a623822c2fcc6b0},\\r\\nissn={08692084},\\r\\npubmed_id={18619204},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Klin. Lab. Diagn.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Landa, S.\",\"Filatov, M.\",\"Arutyunyan, A.\",\"Varfolomeyeva, Y.\"],\"key\":\"Landa200837\",\"id\":\"Landa200837\",\"bibbaseid\":\"landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&partnerID=40&md5=a30b0b5ac1d900684a623822c2fcc6b0\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kuklin\"],\"firstnames\":[\"A.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Islamov\"],\"firstnames\":[\"A.Kh.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stellbrink\"],\"firstnames\":[\"J.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Denisov\"],\"firstnames\":[\"Yu.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Toperverg\"],\"firstnames\":[\"B.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization\",\"journal\":\"Crystallography Reports\",\"year\":\"2008\",\"volume\":\"53\",\"number\":\"1\",\"pages\":\"110-115\",\"doi\":\"10.1134/S1063774508010136\",\"note\":\"cited By 18\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&doi=10.1134%2fS1063774508010136&partnerID=40&md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningrad oblast 188300, Russian Federation; Biofizika Research and Education Center, St. Petersburg State Polytechnic University, Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Joint Institute of Nuclear Research, ul. Zholio-Kyuri 6, Dubna, Moscow Oblast 141980, Russian Federation; Research Centre Jülich, Jülich 52425, Germany\",\"abstract\":\"The chromatin organization in chicken erythrocyte nuclei was studied by small-angle neutron scattering in the scattering-vector range from 1.5 × 10-1 to 10-4 Å-1 with the use of the contrast-variation technique. This scattering-vector range corresponds to linear dimensions from 4 nm to 6 μm and covers the whole hierarchy of chromatin structures, from the nucleosomal structure to the entire nucleus. The results of the present study allowed the following conclusions to be drawn: (1) both the chromatin-protein structure and the structure of the nucleic acid component in chicken erythrocyte nuclei have mass-fractal properties, (2) the structure of the protein component of chromatin exhibits a fractal behavior on scales extending over two orders of magnitude, from the nucleosomal size to the size of an entire nucleus, and (3) the structure of the nucleic acid component of chromatin in chicken erythrocyte nuclei is likewise of a fractal nature and has two levels of organization or two phases with the crossover point at about 300-400 nm. © 2008 Pleiades Publishing, Inc.\",\"funding_details\":\"Ministry of Education and Science of the Russian Federation40.012.1.1.1149\",\"bibtex\":\"@ARTICLE{Lebedev2008110,\\r\\nauthor={Lebedev, D.V. and Filatov, M.V. and Kuklin, A.I. and Islamov, A.Kh. and Stellbrink, J. and Pantina, R.A. and Denisov, Yu.Yu. and Toperverg, B.P. and Isaev-Ivanov, V.V.},\\r\\ntitle={Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization},\\r\\njournal={Crystallography Reports},\\r\\nyear={2008},\\r\\nvolume={53},\\r\\nnumber={1},\\r\\npages={110-115},\\r\\ndoi={10.1134/S1063774508010136},\\r\\nnote={cited By 18},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&doi=10.1134%2fS1063774508010136&partnerID=40&md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningrad oblast 188300, Russian Federation; Biofizika Research and Education Center, St. Petersburg State Polytechnic University, Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Joint Institute of Nuclear Research, ul. Zholio-Kyuri 6, Dubna, Moscow Oblast 141980, Russian Federation; Research Centre Jülich, Jülich 52425, Germany},\\r\\nabstract={The chromatin organization in chicken erythrocyte nuclei was studied by small-angle neutron scattering in the scattering-vector range from 1.5 × 10-1 to 10-4 Å-1 with the use of the contrast-variation technique. This scattering-vector range corresponds to linear dimensions from 4 nm to 6 μm and covers the whole hierarchy of chromatin structures, from the nucleosomal structure to the entire nucleus. The results of the present study allowed the following conclusions to be drawn: (1) both the chromatin-protein structure and the structure of the nucleic acid component in chicken erythrocyte nuclei have mass-fractal properties, (2) the structure of the protein component of chromatin exhibits a fractal behavior on scales extending over two orders of magnitude, from the nucleosomal size to the size of an entire nucleus, and (3) the structure of the nucleic acid component of chromatin in chicken erythrocyte nuclei is likewise of a fractal nature and has two levels of organization or two phases with the crossover point at about 300-400 nm. © 2008 Pleiades Publishing, Inc.},\\r\\nfunding_details={Российский Фонд Фундаментальных Исследований (РФФИ)02-04-49259-a},\\r\\nfunding_details={Ministry of Education and Science of the Russian FederationRNP 2.2.1.1.4663},\\r\\nfunding_details={Ministry of Education and Science of the Russian Federation40.012.1.1.1149},\\r\\n}\",\"author_short\":[\"Lebedev, D.\",\"Filatov, M.\",\"Kuklin, A.\",\"Islamov, A.\",\"Stellbrink, J.\",\"Pantina, R.\",\"Denisov, Y.\",\"Toperverg, B.\",\"Isaev-Ivanov, V.\"],\"key\":\"Lebedev2008110\",\"id\":\"Lebedev2008110\",\"bibbaseid\":\"lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&doi=10.1134%2fS1063774508010136&partnerID=40&md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"book\",\"type\":\"book\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it\",\"journal\":\"From Genome to Proteome: Advances in the Practice & Application of Proteomics\",\"year\":\"2007\",\"pages\":\"453-458\",\"doi\":\"10.1002/9783527613489.ch59\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&doi=10.1002%2f9783527613489.ch59&partnerID=40&md5=41038eec12b571fb984fee210e4729d5\",\"affiliation\":\"Petersburg Nuclear Physics Institute of Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation\",\"abstract\":\"It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA. © 2000 WILEY-VCH Verlag GmbH. All rights reserved.\",\"author_keywords\":\"DNA clearing; Hoechst 33342; Mammalian cells; Two-dimensional Polyacrylamide gel electrophoresis\",\"correspondence_address1\":\"Naryzhny, S.N.; Petersburg Nuclear Physics Institute of Russian Academy of SciencesRussian Federation; email: naryzhny@omrb.pnpi.spb.ru\",\"publisher\":\"Wiley Blackwell\",\"isbn\":\"9783527613489; 9783527301546\",\"language\":\"English\",\"abbrev_source_title\":\"From Genome to Proteome: Adv. in the Pract. & Appl. of Proteomics\",\"document_type\":\"Book Chapter\",\"source\":\"Scopus\",\"bibtex\":\"@BOOK{Naryzhny2007453,\\r\\nauthor={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},\\r\\ntitle={Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it},\\r\\njournal={From Genome to Proteome: Advances in the Practice & Application of Proteomics},\\r\\nyear={2007},\\r\\npages={453-458},\\r\\ndoi={10.1002/9783527613489.ch59},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&doi=10.1002%2f9783527613489.ch59&partnerID=40&md5=41038eec12b571fb984fee210e4729d5},\\r\\naffiliation={Petersburg Nuclear Physics Institute of Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation},\\r\\nabstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA. © 2000 WILEY-VCH Verlag GmbH. All rights reserved.},\\r\\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional Polyacrylamide gel electrophoresis},\\r\\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute of Russian Academy of SciencesRussian Federation; email: naryzhny@omrb.pnpi.spb.ru},\\r\\npublisher={Wiley Blackwell},\\r\\nisbn={9783527613489; 9783527301546},\\r\\nlanguage={English},\\r\\nabbrev_source_title={From Genome to Proteome: Adv. in the Pract. & Appl. of Proteomics},\\r\\ndocument_type={Book Chapter},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Naryzhny, S.\",\"Levina, V.\",\"Varfolomeeva, E.\",\"Drobchenko, E.\",\"Filatov, M.\"],\"key\":\"Naryzhny2007453\",\"id\":\"Naryzhny2007453\",\"bibbaseid\":\"naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&doi=10.1002%2f9783527613489.ch59&partnerID=40&md5=41038eec12b571fb984fee210e4729d5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shtam\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeyeva\"],\"firstnames\":[\"Ye.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression\",\"journal\":\"Voprosy Onkologii\",\"year\":\"2007\",\"volume\":\"53\",\"number\":\"2\",\"pages\":\"194-199\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&partnerID=40&md5=dd7854f4ddd307cb94f5c9ebb3362452\",\"affiliation\":\"B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation\",\"abstract\":\"The study was concerned with identification of predictive value of p53 expression on sensitivity to tamoxifen in breast cancer management. Estrogen receptor-positive cell line MCF-7 was used to establish p53 expression influence on the rate of cell proliferation after tamoxifen. The investigation demonstrated the absence of that effect when p53 was silenced.\",\"correspondence_address1\":\"Shtam, T. A.; B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation\",\"issn\":\"05073758\",\"coden\":\"VOONA\",\"language\":\"Russian\",\"abbrev_source_title\":\"Vopr. Onkol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shtam2007194,\\r\\nauthor={Shtam, T.A. and Pantina, R.A. and Varfolomeyeva, Ye.Yu. and Filatov, M.V.},\\r\\ntitle={Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression},\\r\\njournal={Voprosy Onkologii},\\r\\nyear={2007},\\r\\nvolume={53},\\r\\nnumber={2},\\r\\npages={194-199},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&partnerID=40&md5=dd7854f4ddd307cb94f5c9ebb3362452},\\r\\naffiliation={B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},\\r\\nabstract={The study was concerned with identification of predictive value of p53 expression on sensitivity to tamoxifen in breast cancer management. Estrogen receptor-positive cell line MCF-7 was used to establish p53 expression influence on the rate of cell proliferation after tamoxifen. The investigation demonstrated the absence of that effect when p53 was silenced.},\\r\\ncorrespondence_address1={Shtam, T. A.; B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},\\r\\nissn={05073758},\\r\\ncoden={VOONA},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Vopr. Onkol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shtam, T.\",\"Pantina, R.\",\"Varfolomeyeva, Y.\",\"Filatov, M.\"],\"key\":\"Shtam2007194\",\"id\":\"Shtam2007194\",\"bibbaseid\":\"shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&partnerID=40&md5=dd7854f4ddd307cb94f5c9ebb3362452\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inchina\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis\",\"journal\":\"Ėksperimental'nai&x0361a i klinicheskai&x0361a gastroėnterologii&x0361a = Experimental & clinical gastroenterology\",\"year\":\"2007\",\"number\":\"6\",\"pages\":\"122-125, 131\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&partnerID=40&md5=e4f22788b05bbb2755dfab001ca19b6e\",\"abstract\":\"The authors have studied hepatoprotective actions of new derivatives of 3-hydroxipyridine on an experimental model of toxic hepatitis (140 white mice). Mexidol and berlithion are choosed as the preparations of comparison. The method of light microscopy is used for the exploration of morphological changes. The cytolytic contents activity, catalase activity and the level of MDA have been determined in blood serum. The antitoxic effect is valued by the survival of the animals. It is found that all examined bonds is corrected morphological changes in toxic hepatitis and increased the index of animals survival, which is more expressed than the preparations of comparison.\",\"correspondence_address1\":\"Semenova, E.V.\",\"issn\":\"16828658\",\"pubmed_id\":\"18416106\",\"language\":\"Russian\",\"abbrev_source_title\":\"Eksp Klin Gastroenterol\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2007,\\r\\nauthor={Semenova, E.V. and Inchina, V.I.},\\r\\ntitle={Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis},\\r\\njournal={Ėksperimental'nai&x0361a i klinicheskai&x0361a gastroėnterologii&x0361a = Experimental & clinical gastroenterology},\\r\\nyear={2007},\\r\\nnumber={6},\\r\\npages={122-125, 131},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&partnerID=40&md5=e4f22788b05bbb2755dfab001ca19b6e},\\r\\nabstract={The authors have studied hepatoprotective actions of new derivatives of 3-hydroxipyridine on an experimental model of toxic hepatitis (140 white mice). Mexidol and berlithion are choosed as the preparations of comparison. The method of light microscopy is used for the exploration of morphological changes. The cytolytic contents activity, catalase activity and the level of MDA have been determined in blood serum. The antitoxic effect is valued by the survival of the animals. It is found that all examined bonds is corrected morphological changes in toxic hepatitis and increased the index of animals survival, which is more expressed than the preparations of comparison.},\\r\\ncorrespondence_address1={Semenova, E.V.},\\r\\nissn={16828658},\\r\\npubmed_id={18416106},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Eksp Klin Gastroenterol},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Inchina, V.\"],\"key\":\"Semenova2007\",\"id\":\"Semenova2007\",\"bibbaseid\":\"semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&partnerID=40&md5=e4f22788b05bbb2755dfab001ca19b6e\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"conference\",\"type\":\"conference\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Galich\"],\"firstnames\":[\"N.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Laser fluorescence fluctuation excesses in molecular immunology experiments\",\"journal\":\"Proceedings of SPIE - The International Society for Optical Engineering\",\"year\":\"2007\",\"volume\":\"6597\",\"doi\":\"10.1117/12.726756\",\"art_number\":\"65970L\",\"note\":\"cited By 5; Conference of Nanodesign, Technology, and Computer Simulations ; Conference Date: 19 June 2006 Through 25 June 2006; Conference Code:70585\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&doi=10.1117%2f12.726756&partnerID=40&md5=1e708809433caac2e8f0ce3082166aba\",\"affiliation\":\"St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation; B.P.Konstantinov Petersburg Nuclear Physics Institute, St.Petersburg, 183300, Russian Federation\",\"abstract\":\"A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.\",\"author_keywords\":\"Cytofluorescence histogram; Excesses; Inflammation; Respiratory burst; Statistic analysis\",\"correspondence_address1\":\"Galich, N.E.; St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation\",\"sponsors\":\"SPIE Poland Chapter; SPIE Russia Chapter\",\"address\":\"Olsztyn\",\"issn\":\"0277786X\",\"isbn\":\"0819467332; 9780819467331\",\"coden\":\"PSISD\",\"language\":\"English\",\"abbrev_source_title\":\"Proc SPIE Int Soc Opt Eng\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@CONFERENCE{Galich2007,\\r\\nauthor={Galich, N.E. and Filatov, M.V.},\\r\\ntitle={Laser fluorescence fluctuation excesses in molecular immunology experiments},\\r\\njournal={Proceedings of SPIE - The International Society for Optical Engineering},\\r\\nyear={2007},\\r\\nvolume={6597},\\r\\ndoi={10.1117/12.726756},\\r\\nart_number={65970L},\\r\\nnote={cited By 5; Conference of Nanodesign, Technology, and Computer Simulations ; Conference Date: 19 June 2006 Through 25 June 2006;  Conference Code:70585},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&doi=10.1117%2f12.726756&partnerID=40&md5=1e708809433caac2e8f0ce3082166aba},\\r\\naffiliation={St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation; B.P.Konstantinov Petersburg Nuclear Physics Institute, St.Petersburg, 183300, Russian Federation},\\r\\nabstract={A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.},\\r\\nauthor_keywords={Cytofluorescence histogram;  Excesses;  Inflammation;  Respiratory burst;  Statistic analysis},\\r\\ncorrespondence_address1={Galich, N.E.; St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation},\\r\\nsponsors={SPIE Poland Chapter; SPIE Russia Chapter},\\r\\naddress={Olsztyn},\\r\\nissn={0277786X},\\r\\nisbn={0819467332; 9780819467331},\\r\\ncoden={PSISD},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Proc SPIE Int Soc Opt Eng},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Galich, N.\",\"Filatov, M.\"],\"key\":\"Galich2007\",\"id\":\"Galich2007\",\"bibbaseid\":\"galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&doi=10.1117%2f12.726756&partnerID=40&md5=1e708809433caac2e8f0ce3082166aba\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Carey\"],\"firstnames\":[\"G.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Qi\"],\"firstnames\":[\"X.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Keegan\"],\"firstnames\":[\"A.D.\"],\"suffixes\":[]}],\"title\":\"IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway\",\"journal\":\"Cell Research\",\"year\":\"2007\",\"volume\":\"17\",\"number\":\"11\",\"pages\":\"942-955\",\"doi\":\"10.1038/sj.cr.2007.90\",\"note\":\"cited By 24\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&doi=10.1038%2fsj.cr.2007.90&partnerID=40&md5=9a31d0fb63047d78fac19588b492e312\",\"affiliation\":\"Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland of School of Medicine, Baltimore, MD, United States\",\"abstract\":\"Interleukin-4 (IL-4) promotes lymphocyte survival and protects primary lymphomas from apoptosis. Previous studies reported differential requirements for the signal transducer and activator of transcription 6 (STAT6) and IRS2/phosphatidylinositol 3 kinase (PI-3K) signaling pathways in mediating the IL-4-induced protection from Fas-mediated apoptosis. In this study, we characterized IL-4-activated signals that suppress anti-IgM-mediated apoptosis and growth arrest of CH31, a model B-cell lymphoma line. In CH31, anti-IgM treatment leads to the loss of mitochondrial membrane potential, phospho-Akt, phospho-CDK2, and c-myc protein. These losses are followed by massive induction of p27Kip1 protein expression, cell cycle arrest, and apoptosis. Strikingly, IL-4 treatment prevented or reversed these changes. Furthermore, IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD. IL-4 treatment also induced expression of BclxL, a STAT6-dependent gene. Pharmacologic inhibitors and dominant inhibitory forms of PI-3K and Akt abrogated the anti-apoptotic function of IL-4. These results suggest that the IL-4 receptor activates several signaling pathways, with the Akt pathway playing a major role in suppression of the apoptotic program activated by anti-IgM. © 2007 IBCB, SIBS, CAS. All rights reserved.\",\"author_keywords\":\"Akt; Apoptosis; Cell cycle; IL-4; PI-3 kinase\",\"correspondence_address1\":\"Keegan, A.D.; Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; email: akeegan@som.umaryland.edu\",\"issn\":\"10010602\",\"pubmed_id\":\"17968425\",\"language\":\"English\",\"abbrev_source_title\":\"Cell Res.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Carey2007942,\\r\\nauthor={Carey, G.B. and Semenova, E. and Qi, X. and Keegan, A.D.},\\r\\ntitle={IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway},\\r\\njournal={Cell Research},\\r\\nyear={2007},\\r\\nvolume={17},\\r\\nnumber={11},\\r\\npages={942-955},\\r\\ndoi={10.1038/sj.cr.2007.90},\\r\\nnote={cited By 24},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&doi=10.1038%2fsj.cr.2007.90&partnerID=40&md5=9a31d0fb63047d78fac19588b492e312},\\r\\naffiliation={Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland of School of Medicine, Baltimore, MD, United States},\\r\\nabstract={Interleukin-4 (IL-4) promotes lymphocyte survival and protects primary lymphomas from apoptosis. Previous studies reported differential requirements for the signal transducer and activator of transcription 6 (STAT6) and IRS2/phosphatidylinositol 3 kinase (PI-3K) signaling pathways in mediating the IL-4-induced protection from Fas-mediated apoptosis. In this study, we characterized IL-4-activated signals that suppress anti-IgM-mediated apoptosis and growth arrest of CH31, a model B-cell lymphoma line. In CH31, anti-IgM treatment leads to the loss of mitochondrial membrane potential, phospho-Akt, phospho-CDK2, and c-myc protein. These losses are followed by massive induction of p27Kip1 protein expression, cell cycle arrest, and apoptosis. Strikingly, IL-4 treatment prevented or reversed these changes. Furthermore, IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD. IL-4 treatment also induced expression of BclxL, a STAT6-dependent gene. Pharmacologic inhibitors and dominant inhibitory forms of PI-3K and Akt abrogated the anti-apoptotic function of IL-4. These results suggest that the IL-4 receptor activates several signaling pathways, with the Akt pathway playing a major role in suppression of the apoptotic program activated by anti-IgM. © 2007 IBCB, SIBS, CAS. All rights reserved.},\\r\\nauthor_keywords={Akt;  Apoptosis;  Cell cycle;  IL-4;  PI-3 kinase},\\r\\ncorrespondence_address1={Keegan, A.D.; Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; email: akeegan@som.umaryland.edu},\\r\\nissn={10010602},\\r\\npubmed_id={17968425},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Cell Res.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Carey, G.\",\"Semenova, E.\",\"Qi, X.\",\"Keegan, A.\"],\"key\":\"Carey2007942\",\"id\":\"Carey2007942\",\"bibbaseid\":\"carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&doi=10.1038%2fsj.cr.2007.90&partnerID=40&md5=9a31d0fb63047d78fac19588b492e312\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominsky\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bagyeva\"],\"firstnames\":[\"G.H.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Illarioshkin\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanova-Smolenskaia\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborska\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage\",\"journal\":\"BMC Medical Genetics\",\"year\":\"2007\",\"volume\":\"8\",\"doi\":\"10.1186/1471-2350-8-6\",\"art_number\":\"6\",\"note\":\"cited By 33\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&doi=10.1186%2f1471-2350-8-6&partnerID=40&md5=bb4117dd790d75c9fea41d1ab154724f\",\"affiliation\":\"Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; Department of Neurogenetics, Institute of Neurology, 80 Volokolamskoye Highway, 123367 Moscow, Russian Federation\",\"abstract\":\"Background: One of the causes of Parkinson's disease is mutations in the PARK2 gene. Deletions and duplications of single exons or exon groups account for a large proportion of the gene mutations. Direct detection of these mutations can be used for the diagnosis of Parkinson's disease. Methods: To detect these mutations, we developed an effective technique based on the real-time TaqMan PCR system, which allows us to evaluate the copynumbers of the PARK2 gene exons by comparing the intensity of the amplification signals from some exon of this gene with that of the β-globin gene (the internal control). Results: We analyzed rearrangements in exons 1-12 of the PARK2 gene in 64 patients from Russia with early-onset Parkinson's disease. The frequency of these mutations in our patients was 14%. Conclusion: We have developed a simple, accurate, and reproducible method applicable to the rapid detection of exon rearrangements in the PARK2 gene. It is suitable for the analysis of large patient groups, and it may become the basis for a diagnostic test. © 2007 Shadrina et al; licensee BioMed Central Ltd.\",\"correspondence_address1\":\"Semenova, E.V.; Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; email: semenova-helen@mail.ru\",\"issn\":\"14712350\",\"coden\":\"BMGMA\",\"pubmed_id\":\"17324265\",\"language\":\"English\",\"abbrev_source_title\":\"BMC Med. Genet.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shadrina2007,\\r\\nauthor={Shadrina, M.I. and Semenova, E.V. and Slominsky, P.A. and Bagyeva, G.H. and Illarioshkin, S.N. and Ivanova-Smolenskaia, I.I. and Limborska, S.A.},\\r\\ntitle={Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage},\\r\\njournal={BMC Medical Genetics},\\r\\nyear={2007},\\r\\nvolume={8},\\r\\ndoi={10.1186/1471-2350-8-6},\\r\\nart_number={6},\\r\\nnote={cited By 33},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&doi=10.1186%2f1471-2350-8-6&partnerID=40&md5=bb4117dd790d75c9fea41d1ab154724f},\\r\\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; Department of Neurogenetics, Institute of Neurology, 80 Volokolamskoye Highway, 123367 Moscow, Russian Federation},\\r\\nabstract={Background: One of the causes of Parkinson's disease is mutations in the PARK2 gene. Deletions and duplications of single exons or exon groups account for a large proportion of the gene mutations. Direct detection of these mutations can be used for the diagnosis of Parkinson's disease. Methods: To detect these mutations, we developed an effective technique based on the real-time TaqMan PCR system, which allows us to evaluate the copynumbers of the PARK2 gene exons by comparing the intensity of the amplification signals from some exon of this gene with that of the β-globin gene (the internal control). Results: We analyzed rearrangements in exons 1-12 of the PARK2 gene in 64 patients from Russia with early-onset Parkinson's disease. The frequency of these mutations in our patients was 14%. Conclusion: We have developed a simple, accurate, and reproducible method applicable to the rapid detection of exon rearrangements in the PARK2 gene. It is suitable for the analysis of large patient groups, and it may become the basis for a diagnostic test. © 2007 Shadrina et al; licensee BioMed Central Ltd.},\\r\\ncorrespondence_address1={Semenova, E.V.; Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; email: semenova-helen@mail.ru},\\r\\nissn={14712350},\\r\\ncoden={BMGMA},\\r\\npubmed_id={17324265},\\r\\nlanguage={English},\\r\\nabbrev_source_title={BMC Med. Genet.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shadrina, M.\",\"Semenova, E.\",\"Slominsky, P.\",\"Bagyeva, G.\",\"Illarioshkin, S.\",\"Ivanova-Smolenskaia, I.\",\"Limborska, S.\"],\"key\":\"Shadrina2007\",\"id\":\"Shadrina2007\",\"bibbaseid\":\"shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&doi=10.1186%2f1471-2350-8-6&partnerID=40&md5=bb4117dd790d75c9fea41d1ab154724f\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hurt\"],\"firstnames\":[\"C.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Xiang\"],\"firstnames\":[\"Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Dell'Acqua\"],\"firstnames\":[\"M.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zhang\"],\"firstnames\":[\"Q.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tsien\"],\"firstnames\":[\"R.W.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kobilka\"],\"firstnames\":[\"B.K.\"],\"suffixes\":[]}],\"title\":\"Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes\",\"journal\":\"Journal of Cell Biology\",\"year\":\"2007\",\"volume\":\"176\",\"number\":\"4\",\"pages\":\"521-533\",\"doi\":\"10.1083/jcb.200604167\",\"note\":\"cited By 64\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&doi=10.1083%2fjcb.200604167&partnerID=40&md5=b033b7ab6098f89f08e082e6f4d61463\",\"affiliation\":\"Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; Department of Pharmacology, University of Colorado, Denver and Health Sciences Center, Aurora, CO 80045, United States; Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States\",\"abstract\":\"The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo. © The Rockefeller University Press.\",\"correspondence_address1\":\"Kobilka, B.K.; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; email: kobilka@stanford.edu\",\"issn\":\"00219525\",\"coden\":\"JCLBA\",\"pubmed_id\":\"17296797\",\"language\":\"English\",\"abbrev_source_title\":\"J. Cell Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shcherbakova2007521,\\r\\nauthor={Shcherbakova, O.G. and Hurt, C.M. and Xiang, Y. and Dell'Acqua, M.L. and Zhang, Q. and Tsien, R.W. and Kobilka, B.K.},\\r\\ntitle={Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes},\\r\\njournal={Journal of Cell Biology},\\r\\nyear={2007},\\r\\nvolume={176},\\r\\nnumber={4},\\r\\npages={521-533},\\r\\ndoi={10.1083/jcb.200604167},\\r\\nnote={cited By 64},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&doi=10.1083%2fjcb.200604167&partnerID=40&md5=b033b7ab6098f89f08e082e6f4d61463},\\r\\naffiliation={Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; Department of Pharmacology, University of Colorado, Denver and Health Sciences Center, Aurora, CO 80045, United States; Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States},\\r\\nabstract={The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo. © The Rockefeller University Press.},\\r\\ncorrespondence_address1={Kobilka, B.K.; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; email: kobilka@stanford.edu},\\r\\nissn={00219525},\\r\\ncoden={JCLBA},\\r\\npubmed_id={17296797},\\r\\nlanguage={English},\\r\\nabbrev_source_title={J. Cell Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shcherbakova, O.\",\"Hurt, C.\",\"Xiang, Y.\",\"Dell'Acqua, M.\",\"Zhang, Q.\",\"Tsien, R.\",\"Kobilka, B.\"],\"key\":\"Shcherbakova2007521\",\"id\":\"Shcherbakova2007521\",\"bibbaseid\":\"shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&doi=10.1083%2fjcb.200604167&partnerID=40&md5=b033b7ab6098f89f08e082e6f4d61463\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominsky\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zherebtsova\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levitsky\"],\"firstnames\":[\"G.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levitskaya\"],\"firstnames\":[\"N.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Alekhin\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Serdyuk\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Skvortsova\"],\"firstnames\":[\"V.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborska\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population\",\"journal\":\"Balkan Journal of Medical Genetics\",\"year\":\"2006\",\"volume\":\"9\",\"number\":\"1-2\",\"pages\":\"31-41\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&partnerID=40&md5=3032bf178ce2c5b63c1c8996952a79f9\",\"affiliation\":\"Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; Department of Fundamental and Clinical Neurology, Russian State Medical University, Moscow, Russian Federation\",\"abstract\":\"Motor neuron disease (MND) results in selective degeneration of motor neurons of the cerebral cortex, brain stem and spinal cord. It is a complex disease in the pathogenesis of which many genetic systems may be involved. A significant etiological factor of MND may be oxygen free radicals which damage neuronal cells when present in high concentrations. In detoxification processes, free radicals may be formed that become transformed into non toxic products. The major participants of these processes are the cytochromes P-450 CYP2E1, CYP2D6, the glutathione-S-transferases GSTM1, GSTT1, GSTP1, and the N-acetyltransferase NAT2. To investigate their role in the development of MND, we have studied polymorphisms in these genes in 75 Russian patients with MND and 105 controls. We have established a statistical distinction in frequencies of CYP2E1*1D, and GSTM1 (0/0) homozygotes between the patient and the control samples. Whereas the analysis of the CYP2D6, GSTT1, GSTP1 and NAT2 gene polymorphisms has revealed no differences, the GSTP1*A/GSTP1*A genotype was associated with classical upper and lower motor neuron involvement. The GSTP1*B allele was associated predominantly with lower and upper motor neuron involvement. We propose that the genes of phases I and II of the detoxification system, CYP2E1, GSTP1, and GSTM1, participate in the development of sporadic MND in patients in Russia.\",\"author_keywords\":\"Detoxification processes; Gene polymorphisms; Motor neuron disease (MND)\",\"correspondence_address1\":\"Shadrina, M.I.; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; email: shadrina@img.ras.ru\",\"issn\":\"13110160\",\"coden\":\"BJMGF\",\"language\":\"English\",\"abbrev_source_title\":\"Balk. J. Med. Genet.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shadrina200631,\\r\\nauthor={Shadrina, M.I. and Slominsky, P.A. and Zherebtsova, A.L. and Levitsky, G.N. and Levitskaya, N.I. and Alekhin, A.V. and Semenova, E.V. and Serdyuk, A.V. and Skvortsova, V.L. and Limborska, S.A.},\\r\\ntitle={Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population},\\r\\njournal={Balkan Journal of Medical Genetics},\\r\\nyear={2006},\\r\\nvolume={9},\\r\\nnumber={1-2},\\r\\npages={31-41},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&partnerID=40&md5=3032bf178ce2c5b63c1c8996952a79f9},\\r\\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; Department of Fundamental and Clinical Neurology, Russian State Medical University, Moscow, Russian Federation},\\r\\nabstract={Motor neuron disease (MND) results in selective degeneration of motor neurons of the cerebral cortex, brain stem and spinal cord. It is a complex disease in the pathogenesis of which many genetic systems may be involved. A significant etiological factor of MND may be oxygen free radicals which damage neuronal cells when present in high concentrations. In detoxification processes, free radicals may be formed that become transformed into non toxic products. The major participants of these processes are the cytochromes P-450 CYP2E1, CYP2D6, the glutathione-S-transferases GSTM1, GSTT1, GSTP1, and the N-acetyltransferase NAT2. To investigate their role in the development of MND, we have studied polymorphisms in these genes in 75 Russian patients with MND and 105 controls. We have established a statistical distinction in frequencies of CYP2E1*1D, and GSTM1 (0/0) homozygotes between the patient and the control samples. Whereas the analysis of the CYP2D6, GSTT1, GSTP1 and NAT2 gene polymorphisms has revealed no differences, the GSTP1*A/GSTP1*A genotype was associated with classical upper and lower motor neuron involvement. The GSTP1*B allele was associated predominantly with lower and upper motor neuron involvement. We propose that the genes of phases I and II of the detoxification system, CYP2E1, GSTP1, and GSTM1, participate in the development of sporadic MND in patients in Russia.},\\r\\nauthor_keywords={Detoxification processes;  Gene polymorphisms;  Motor neuron disease (MND)},\\r\\ncorrespondence_address1={Shadrina, M.I.; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; email: shadrina@img.ras.ru},\\r\\nissn={13110160},\\r\\ncoden={BJMGF},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Balk. J. Med. Genet.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shadrina, M.\",\"Slominsky, P.\",\"Zherebtsova, A.\",\"Levitsky, G.\",\"Levitskaya, N.\",\"Alekhin, A.\",\"Semenova, E.\",\"Serdyuk, A.\",\"Skvortsova, V.\",\"Limborska, S.\"],\"key\":\"Shadrina200631\",\"id\":\"Shadrina200631\",\"bibbaseid\":\"shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&partnerID=40&md5=3032bf178ce2c5b63c1c8996952a79f9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Brum\"],\"firstnames\":[\"P.C.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hurt\"],\"firstnames\":[\"C.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kobilka\"],\"firstnames\":[\"B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Angelotti\"],\"firstnames\":[\"T.\"],\"suffixes\":[]}],\"title\":\"Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons\",\"journal\":\"Neuropharmacology\",\"year\":\"2006\",\"volume\":\"51\",\"number\":\"3\",\"pages\":\"397-413\",\"doi\":\"10.1016/j.neuropharm.2006.03.032\",\"note\":\"cited By 28\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&doi=10.1016%2fj.neuropharm.2006.03.032&partnerID=40&md5=14ace5ce3f4045c0266da4c7f86c81d5\",\"affiliation\":\"Laboratório de Fisiologia do Exercício, Escola de Educação Física e Esporte, Universidade de Sao Paulo, Av. Prof. Mello Moraes 65, 05508-900 Sao Paulo, SP, Brazil; Department of Molecular and Cellular Physiology, Stanford University Medical School, 157 Beckman Center, 279 Campus Drive, Stanford, CA 94305, United States; Department of Anesthesia, Stanford University Medical School, Stanford, CA 94305, United States\",\"abstract\":\"Previous research suggested that α2A and α2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed α2A and α2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, α2A and α2CARs could be readily detected in SGN from wild-type mice. By Day 8, α2AARs were targeted to cell body, as well as axonal and dendritic sites, whereas α2CARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, α2CARs had relocated to somatodendritic and axonal sites and, unlike α2AARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for α2ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous α2A and α2CAR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of α2CARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between α2A and α2CARs in SGN. © 2006 Elsevier Ltd. All rights reserved.\",\"author_keywords\":\"α2 adrenergic receptor; Dexmedetomidine; SV2; Sympathetic ganglion neuron; Trafficking\",\"funding_details\":\"98/14765-7\",\"bibtex\":\"@ARTICLE{Brum2006397,\\r\\nauthor={Brum, P.C. and Hurt, C.M. and Shcherbakova, O.G. and Kobilka, B. and Angelotti, T.},\\r\\ntitle={Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons},\\r\\njournal={Neuropharmacology},\\r\\nyear={2006},\\r\\nvolume={51},\\r\\nnumber={3},\\r\\npages={397-413},\\r\\ndoi={10.1016/j.neuropharm.2006.03.032},\\r\\nnote={cited By 28},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&doi=10.1016%2fj.neuropharm.2006.03.032&partnerID=40&md5=14ace5ce3f4045c0266da4c7f86c81d5},\\r\\naffiliation={Laboratório de Fisiologia do Exercício, Escola de Educação Física e Esporte, Universidade de Sao Paulo, Av. Prof. Mello Moraes 65, 05508-900 Sao Paulo, SP, Brazil; Department of Molecular and Cellular Physiology, Stanford University Medical School, 157 Beckman Center, 279 Campus Drive, Stanford, CA 94305, United States; Department of Anesthesia, Stanford University Medical School, Stanford, CA 94305, United States},\\r\\nabstract={Previous research suggested that α2A and α2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed α2A and α2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, α2A and α2CARs could be readily detected in SGN from wild-type mice. By Day 8, α2AARs were targeted to cell body, as well as axonal and dendritic sites, whereas α2CARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, α2CARs had relocated to somatodendritic and axonal sites and, unlike α2AARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for α2ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous α2A and α2CAR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of α2CARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between α2A and α2CARs in SGN. © 2006 Elsevier Ltd. All rights reserved.},\\r\\nauthor_keywords={α2 adrenergic receptor;  Dexmedetomidine;  SV2;  Sympathetic ganglion neuron;  Trafficking},\\r\\nfunding_details={GM07365},\\r\\nfunding_details={98/14765-7},\\r\\n}\",\"author_short\":[\"Brum, P.\",\"Hurt, C.\",\"Shcherbakova, O.\",\"Kobilka, B.\",\"Angelotti, T.\"],\"key\":\"Brum2006397\",\"id\":\"Brum2006397\",\"bibbaseid\":\"brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&doi=10.1016%2fj.neuropharm.2006.03.032&partnerID=40&md5=14ace5ce3f4045c0266da4c7f86c81d5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Peshekhonov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli\",\"journal\":\"Molekuliarnaia biologiia.\",\"year\":\"2006\",\"volume\":\"40\",\"number\":\"4\",\"pages\":\"669-683\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&partnerID=40&md5=7eb6533d775986f49cbe70542290ec2d\",\"abstract\":\"A modified nucleotide on the 3'-side of the anticodon loop of tRNA is one of the most important structure element regulating codon-anticodone interaction on the ribosome owing to the stacking interaction with the stack of codon-anticodon bases. The presence and identity (pyrimidine, purine or modified purine) of this nucleotide has an essential influence on the energy of the stacking interaction on A- and P-sites of the ribosome. There is a significant influence of the 37-modification by itself on the P-site, whereas there is no such one on the A-site of the ribosome. Comparison of binding enthalpies of tRNA interactions on the P- or A-site of the ribosome with the binding enthalpies of the complex of two tRNAs with the complementary anticodones suggests that the ribosome by itself significantly endows in the thermodynamics of codon-anticodon complex formation. It happens by additional ribosomal interactions with the molecule of tRNA or indirectly by the stabilization of codon-anticodon conformation. In addition to the stacking, tRNA binding in the A and P sites is futher stabilized by the interactions involving some magnesium ions. The number of them involved in those interactions strongly depends on the nucleotide identity in the 37-position of tRNA anticodon loop.\",\"correspondence_address1\":\"Konevega, A.L.\",\"issn\":\"00268984\",\"pubmed_id\":\"16913226\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mol. Biol. (Mosk.)\",\"document_type\":\"Review\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Konevega2006669,\\r\\nauthor={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Peshekhonov, A.V. and Katunin, V.I.},\\r\\ntitle={The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli},\\r\\njournal={Molekuliarnaia biologiia.},\\r\\nyear={2006},\\r\\nvolume={40},\\r\\nnumber={4},\\r\\npages={669-683},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&partnerID=40&md5=7eb6533d775986f49cbe70542290ec2d},\\r\\nabstract={A modified nucleotide on the 3'-side of the anticodon loop of tRNA is one of the most important structure element regulating codon-anticodone interaction on the ribosome owing to the stacking interaction with the stack of codon-anticodon bases. The presence and identity (pyrimidine, purine or modified purine) of this nucleotide has an essential influence on the energy of the stacking interaction on A- and P-sites of the ribosome. There is a significant influence of the 37-modification by itself on the P-site, whereas there is no such one on the A-site of the ribosome. Comparison of binding enthalpies of tRNA interactions on the P- or A-site of the ribosome with the binding enthalpies of the complex of two tRNAs with the complementary anticodones suggests that the ribosome by itself significantly endows in the thermodynamics of codon-anticodon complex formation. It happens by additional ribosomal interactions with the molecule of tRNA or indirectly by the stabilization of codon-anticodon conformation. In addition to the stacking, tRNA binding in the A and P sites is futher stabilized by the interactions involving some magnesium ions. The number of them involved in those interactions strongly depends on the nucleotide identity in the 37-position of tRNA anticodon loop.},\\r\\ncorrespondence_address1={Konevega, A.L.},\\r\\nissn={00268984},\\r\\npubmed_id={16913226},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mol. Biol. (Mosk.)},\\r\\ndocument_type={Review},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Konevega, A.\",\"Soboleva, N.\",\"Makhno, V.\",\"Peshekhonov, A.\",\"Katunin, V.\"],\"key\":\"Konevega2006669\",\"id\":\"Konevega2006669\",\"bibbaseid\":\"konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&partnerID=40&md5=7eb6533d775986f49cbe70542290ec2d\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Peshekhonov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"Effect of modification of tRNA nucleotide 37 on the tRNA interaction with the A and P sites of the Escherichia coli 70S ribosome\",\"journal\":\"Molecular Biology\",\"year\":\"2006\",\"volume\":\"40\",\"number\":\"4\",\"pages\":\"597-610\",\"doi\":\"10.1134/S0026893306040121\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&doi=10.1134%2fS0026893306040121&partnerID=40&md5=68bb1ea3d0ae09fa1886bf15fc3f2c82\",\"affiliation\":\"Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, 188300, Russian Federation\",\"abstract\":\"The modified nucleotide 3′ of the tRNA anticodon is an important structural element that regulates the codon-anticodon interaction in the ribosome by stacking with codon-anticodon bases. The presence and identity (pyrimidine, purine, or modified purine) of this nucleotide significantly affects the energy of stacking in the A and P sites of the ribosome. Modification of nucleotide 37 does not contribute to stacking in the A site of the 70S ribosome, while its effect is substantial in the P site. The enthalpies of tRNA interactions with the A and P sites in the ribosome are similar and considerably lower than the enthalpy of the interactions of two tRNAs with the cognate anticodons in solution, suggesting that the ribosome contributes to the enthalpy-related portion of the free energy of tRNA binding by directly forming additional interactions with tRNA or by indirectly stabilizing the conformation of the codon-anticodon complex. In addition to stacking, tRNA binding in the A and P sites is further stabilized by interactions that involve magnesium ions. The number of ions involved in the formation of the tRNA-ribosome complex depends on the identity of tRNA nucleotide 37. © Pleiades Publishing, Inc., 2006.\",\"author_keywords\":\"70S ribosome; A site; Codon-anticodon interaction; P site; tRNA modification; tRNAPhe; Y base\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)06-04-49614, 01-04-49252\",\"key\":\"Konevega2006597\",\"id\":\"Konevega2006597\",\"bibbaseid\":\"anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&doi=10.1134%2fS0026893306040121&partnerID=40&md5=68bb1ea3d0ae09fa1886bf15fc3f2c82\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Zherebtsova\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levitskiǐ\"],\"firstnames\":[\"G.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Alekhin\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominskiǐ\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Skvortsova\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborskaia\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population\",\"journal\":\"Genetika.\",\"year\":\"2006\",\"volume\":\"42\",\"number\":\"1\",\"pages\":\"104-109\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&partnerID=40&md5=9d531826cec7c322a8080b0ec3f07a61\",\"abstract\":\"Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND.\",\"correspondence_address1\":\"Zherebtsova, A.L.\",\"issn\":\"00166758\",\"pubmed_id\":\"16523673\",\"language\":\"Russian\",\"abbrev_source_title\":\"Genetika\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Zherebtsova2006104,\\r\\nauthor={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitskiǐ, G.N. and Alekhin, A.V. and Slominskiǐ, P.A. and Skvortsova, V.I. and Limborskaia, S.A.},\\r\\ntitle={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population},\\r\\njournal={Genetika.},\\r\\nyear={2006},\\r\\nvolume={42},\\r\\nnumber={1},\\r\\npages={104-109},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&partnerID=40&md5=9d531826cec7c322a8080b0ec3f07a61},\\r\\nabstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND.},\\r\\ncorrespondence_address1={Zherebtsova, A.L.},\\r\\nissn={00166758},\\r\\npubmed_id={16523673},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Genetika},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Zherebtsova, A.\",\"Shadrina, M.\",\"Semenova, E.\",\"Levitskiǐ, G.\",\"Alekhin, A.\",\"Slominskiǐ, P.\",\"Skvortsova, V.\",\"Limborskaia, S.\"],\"key\":\"Zherebtsova2006104\",\"id\":\"Zherebtsova2006104\",\"bibbaseid\":\"zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&partnerID=40&md5=9d531826cec7c322a8080b0ec3f07a61\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Zherebtsova\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shadrina\"],\"firstnames\":[\"M.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levitsky\"],\"firstnames\":[\"G.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Alekhin\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Slominsky\"],\"firstnames\":[\"P.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Skvortsova\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Limborska\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]}],\"title\":\"Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population\",\"journal\":\"Russian Journal of Genetics\",\"year\":\"2006\",\"volume\":\"42\",\"number\":\"1\",\"pages\":\"89-93\",\"doi\":\"10.1134/S1022795406010133\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&doi=10.1134%2fS1022795406010133&partnerID=40&md5=480e9bf567ea472b2cd6ccec20a9d4c5\",\"affiliation\":\"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russian Federation; Russian State Medical University, Moscow, 129327, Russian Federation\",\"abstract\":\"Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND. © Pleiades Publishing, Inc., 2006.\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)04-04-49 124a, 02-04-49 076\",\"bibtex\":\"@ARTICLE{Zherebtsova200689,\\r\\nauthor={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitsky, G.N. and Alekhin, A.V. and Slominsky, P.A. and Skvortsova, V.I. and Limborska, S.A.},\\r\\ntitle={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population},\\r\\njournal={Russian Journal of Genetics},\\r\\nyear={2006},\\r\\nvolume={42},\\r\\nnumber={1},\\r\\npages={89-93},\\r\\ndoi={10.1134/S1022795406010133},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&doi=10.1134%2fS1022795406010133&partnerID=40&md5=480e9bf567ea472b2cd6ccec20a9d4c5},\\r\\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russian Federation; Russian State Medical University, Moscow, 129327, Russian Federation},\\r\\nabstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND. © Pleiades Publishing, Inc., 2006.},\\r\\nfunding_details={Российский Фонд Фундаментальных Исследований (РФФИ)04-04-49 124a, 02-04-49 076},\\r\\n}\",\"author_short\":[\"Zherebtsova, A.\",\"Shadrina, M.\",\"Semenova, E.\",\"Levitsky, G.\",\"Alekhin, A.\",\"Slominsky, P.\",\"Skvortsova, V.\",\"Limborska, S.\"],\"key\":\"Zherebtsova200689\",\"id\":\"Zherebtsova200689\",\"bibbaseid\":\"zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&doi=10.1134%2fS1022795406010133&partnerID=40&md5=480e9bf567ea472b2cd6ccec20a9d4c5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Lebedev\"],\"firstnames\":[\"D.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kuklin\"],\"firstnames\":[\"A.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Islamov\"],\"firstnames\":[\"A.Kh.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kentzinger\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Toperverg\"],\"firstnames\":[\"B.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Isaev-Ivanov\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]}],\"title\":\"Fractal nature of chromatin organization in interphase chicken erythrocyte nuclei: DNA structure exhibits biphasic fractal properties\",\"journal\":\"FEBS Letters\",\"year\":\"2005\",\"volume\":\"579\",\"number\":\"6\",\"pages\":\"1465-1468\",\"doi\":\"10.1016/j.febslet.2005.01.052\",\"note\":\"cited By 59\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&doi=10.1016%2fj.febslet.2005.01.052&partnerID=40&md5=3dd6734c8bbdb51d6d3bff6a1daf5aad\",\"affiliation\":\"Div. of Molec. and Radiat. Biophys., Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russian Federation; Inst. F. Festkörperforschung, Forschungszentrum Jülich, Jülich, Germany\",\"abstract\":\"Arrangement of chromatin in intact chicken erythrocyte nuclei was investigated by small angle neutron scattering. The scattering spectra have revealed that on the scales between 15 nm and 1.5 μm the interior of the nucleus exhibited properties of a mass fractal. The fractal dimension of the protein component of cell nucleus held constant at approximately 2.5, while the DNA organization was biphasic, with the fractal dimension slightly higher than 2 on the scales smaller than 300 nm and approaching 3 on the larger scales. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.\",\"author_keywords\":\"Cell nucleus; Chromatin structure; Small angle neutron scattering\",\"funding_details\":\"Ministry of Education and Science of the Russian Federation40.012.1.1.1149\",\"key\":\"Lebedev20051465\",\"id\":\"Lebedev20051465\",\"bibbaseid\":\"anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&doi=10.1016%2fj.febslet.2005.01.052&partnerID=40&md5=3dd6734c8bbdb51d6d3bff6a1daf5aad\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ivanov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bakhlanova\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil'noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.]\",\"journal\":\"Tsitologiia\",\"year\":\"2004\",\"volume\":\"46\",\"number\":\"8\",\"pages\":\"740-747\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&partnerID=40&md5=448c2f76d3cf6675cc6b1eff278aefab\",\"abstract\":\"The phenomenon of loosing exogenic DNA from the mammalian somatic cell genome is under investigation. It is found that foreign DNA incorporated into cell genome as a result of transfection by electrophoretion may be lost with the frequency from 1/100 up to 1/100 000 per cell division during cultivation. This effect is not dependent of the nature of cell line and vector DNA. It is actual for different cell lines: A23, human fibroblasts AG 11395, murine embryonic line F9, and for different plasmid vectors: p16, p.39, pATR4 and pcDNA3.1-Higr (WRN). Integration of pDNA into genome and the following loosing of this DNA is registered by selection markers G418 and hygromycin B resistance and gancyclovir sensibility. The presence of foreign DNA in the genome was controlled by PCR. It is found that true foreign DNA deletion from the genome takes place rather than gene expression changes. For closely linked plasmid genes deletion of both genes at once as well as loosing any one gene separately is shown. Thus, the phenomenon of selective deletion of exogenic DNA from genome has been demonstrated for different mammalian cells.\",\"correspondence_address1\":\"Ivanov, A.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"15598021\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ivanov2004740,\\r\\nauthor={Ivanov, A.V. and Bakhlanova, I.V. and Pantina, R.A. and Filatov, M.V.},\\r\\ntitle={Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil'noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.]},\\r\\njournal={Tsitologiia},\\r\\nyear={2004},\\r\\nvolume={46},\\r\\nnumber={8},\\r\\npages={740-747},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&partnerID=40&md5=448c2f76d3cf6675cc6b1eff278aefab},\\r\\nabstract={The phenomenon of loosing exogenic DNA from the mammalian somatic cell genome is under investigation. It is found that foreign DNA incorporated into cell genome as a result of transfection by electrophoretion may be lost with the frequency from 1/100 up to 1/100 000 per cell division during cultivation. This effect is not dependent of the nature of cell line and vector DNA. It is actual for different cell lines: A23, human fibroblasts AG 11395, murine embryonic line F9, and for different plasmid vectors: p16, p.39, pATR4 and pcDNA3.1-Higr (WRN). Integration of pDNA into genome and the following loosing of this DNA is registered by selection markers G418 and hygromycin B resistance and gancyclovir sensibility. The presence of foreign DNA in the genome was controlled by PCR. It is found that true foreign DNA deletion from the genome takes place rather than gene expression changes. For closely linked plasmid genes deletion of both genes at once as well as loosing any one gene separately is shown. Thus, the phenomenon of selective deletion of exogenic DNA from genome has been demonstrated for different mammalian cells.},\\r\\ncorrespondence_address1={Ivanov, A.V.},\\r\\nissn={00413771},\\r\\npubmed_id={15598021},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ivanov, A.\",\"Bakhlanova, I.\",\"Pantina, R.\",\"Filatov, M.\"],\"key\":\"Ivanov2004740\",\"id\":\"Ivanov2004740\",\"bibbaseid\":\"ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&partnerID=40&md5=448c2f76d3cf6675cc6b1eff278aefab\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenkov\"],\"firstnames\":[\"Y.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Wintermeyer\"],\"firstnames\":[\"W.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Rodnina\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions\",\"journal\":\"RNA\",\"year\":\"2004\",\"volume\":\"10\",\"number\":\"1\",\"pages\":\"90-101\",\"doi\":\"10.1261/rna.5142404\",\"note\":\"cited By 73\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&doi=10.1261%2frna.5142404&partnerID=40&md5=c0ac9c400bbb3e2d3a57147153225962\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany; Institute of Physical Biochemistry, University of Witten/Herdecke, 58448 Witten, Germany\",\"abstract\":\"The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNAPhe binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNAPhe with hypermodified G (wybutine), or of unmodified peptidyl-tRNAPhe transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg 2+ ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg2+ -dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon-anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg2+ binding, and interactions with 16S rRNA.\",\"author_keywords\":\"Ribosome decoding; Translation; TRNA conformational change; TRNA modifications; TRNA selection\",\"correspondence_address1\":\"Katunin, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; email: katunin@omrb.pnpi.spb.ru\",\"issn\":\"13558382\",\"coden\":\"RNARF\",\"pubmed_id\":\"14681588\",\"language\":\"English\",\"abbrev_source_title\":\"RNA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Konevega200490,\\r\\nauthor={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Semenkov, Y.P. and Wintermeyer, W. and Rodnina, M.V. and Katunin, V.I.},\\r\\ntitle={Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions},\\r\\njournal={RNA},\\r\\nyear={2004},\\r\\nvolume={10},\\r\\nnumber={1},\\r\\npages={90-101},\\r\\ndoi={10.1261/rna.5142404},\\r\\nnote={cited By 73},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&doi=10.1261%2frna.5142404&partnerID=40&md5=c0ac9c400bbb3e2d3a57147153225962},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany; Institute of Physical Biochemistry, University of Witten/Herdecke, 58448 Witten, Germany},\\r\\nabstract={The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNAPhe binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNAPhe with hypermodified G (wybutine), or of unmodified peptidyl-tRNAPhe transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg 2+ ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg2+ -dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon-anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg2+ binding, and interactions with 16S rRNA.},\\r\\nauthor_keywords={Ribosome decoding;  Translation;  TRNA conformational change;  TRNA modifications;  TRNA selection},\\r\\ncorrespondence_address1={Katunin, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; email: katunin@omrb.pnpi.spb.ru},\\r\\nissn={13558382},\\r\\ncoden={RNARF},\\r\\npubmed_id={14681588},\\r\\nlanguage={English},\\r\\nabbrev_source_title={RNA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Konevega, A.\",\"Soboleva, N.\",\"Makhno, V.\",\"Semenkov, Y.\",\"Wintermeyer, W.\",\"Rodnina, M.\",\"Katunin, V.\"],\"key\":\"Konevega200490\",\"id\":\"Konevega200490\",\"bibbaseid\":\"konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&doi=10.1261%2frna.5142404&partnerID=40&md5=c0ac9c400bbb3e2d3a57147153225962\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenkov\"],\"firstnames\":[\"Yu.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome\",\"journal\":\"Molekulyarnaya Biologiya\",\"year\":\"2003\",\"volume\":\"37\",\"number\":\"1\",\"pages\":\"121-127\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&partnerID=40&md5=5c8ad0bf4218457eab4cbe1a75ed3345\",\"affiliation\":\"Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation\",\"abstract\":\"To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA K +Y Phe and Phe-tRNA K -Y Phe ) with the A site of complex [70S · poly(U) · deacylated tRNA Phe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA K +Y Phe showed, removal of the Y base decreased the association constant of Phe-tRNA K -Y Phe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA Phe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA K -Y Phe but not for Phe-tRNA K +Y Phe . Thus, the modified nucleotide 3′ of the Phe-tRNA Phe anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.\",\"correspondence_address1\":\"Soboleva, N.G.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation\",\"issn\":\"00268984\",\"coden\":\"MOBIB\",\"pubmed_id\":\"12624954\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mol. Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Soboleva2003121,\\r\\nauthor={Soboleva, N.G. and Makhno, V.I. and Konevega, A.L. and Semenkov, Yu.P. and Katunin, V.I.},\\r\\ntitle={The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome},\\r\\njournal={Molekulyarnaya Biologiya},\\r\\nyear={2003},\\r\\nvolume={37},\\r\\nnumber={1},\\r\\npages={121-127},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&partnerID=40&md5=5c8ad0bf4218457eab4cbe1a75ed3345},\\r\\naffiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},\\r\\nabstract={To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA K +Y Phe and Phe-tRNA K -Y Phe ) with the A site of complex [70S · poly(U) · deacylated tRNA Phe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA K +Y Phe showed, removal of the Y base decreased the association constant of Phe-tRNA K -Y Phe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA Phe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA K -Y Phe but not for Phe-tRNA K +Y Phe . Thus, the modified nucleotide 3′ of the Phe-tRNA Phe anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.},\\r\\ncorrespondence_address1={Soboleva, N.G.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},\\r\\nissn={00268984},\\r\\ncoden={MOBIB},\\r\\npubmed_id={12624954},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mol. Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Soboleva, N.\",\"Makhno, V.\",\"Konevega, A.\",\"Semenkov, Y.\",\"Katunin, V.\"],\"key\":\"Soboleva2003121\",\"id\":\"Soboleva2003121\",\"bibbaseid\":\"soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&partnerID=40&md5=5c8ad0bf4218457eab4cbe1a75ed3345\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Marzi\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Knight\"],\"firstnames\":[\"W.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Brandi\"],\"firstnames\":[\"L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Caserta\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Hill\"],\"firstnames\":[\"W.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Gualerzi\"],\"firstnames\":[\"C.O.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lodmell\"],\"firstnames\":[\"J.S.\"],\"suffixes\":[]}],\"title\":\"Ribosomal localization of translation initiation factor IF2\",\"journal\":\"RNA\",\"year\":\"2003\",\"volume\":\"9\",\"number\":\"8\",\"pages\":\"958-969\",\"doi\":\"10.1261/rna.2116303\",\"note\":\"cited By 43\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&doi=10.1261%2frna.2116303&partnerID=40&md5=b40491c4a5016c07d44c5c9308bbc767\",\"affiliation\":\"Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino (MC), Italy; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States\",\"abstract\":\"Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit's factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.\",\"author_keywords\":\"BABE; fMet-tRNA; IF2; Phenanthroline; Ribosome topography; Tethered nuclease; Translation initiation\",\"correspondence_address1\":\"Lodmell, J.S.; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States; email: lodmell@selway.umt.edu\",\"issn\":\"13558382\",\"coden\":\"RNARF\",\"pubmed_id\":\"12869707\",\"language\":\"English\",\"abbrev_source_title\":\"RNA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Marzi2003958,\\r\\nauthor={Marzi, S. and Knight, W. and Brandi, L. and Caserta, E. and Soboleva, N. and Hill, W.E. and Gualerzi, C.O. and Lodmell, J.S.},\\r\\ntitle={Ribosomal localization of translation initiation factor IF2},\\r\\njournal={RNA},\\r\\nyear={2003},\\r\\nvolume={9},\\r\\nnumber={8},\\r\\npages={958-969},\\r\\ndoi={10.1261/rna.2116303},\\r\\nnote={cited By 43},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&doi=10.1261%2frna.2116303&partnerID=40&md5=b40491c4a5016c07d44c5c9308bbc767},\\r\\naffiliation={Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino (MC), Italy; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States},\\r\\nabstract={Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit's factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.},\\r\\nauthor_keywords={BABE;  fMet-tRNA;  IF2;  Phenanthroline;  Ribosome topography;  Tethered nuclease;  Translation initiation},\\r\\ncorrespondence_address1={Lodmell, J.S.; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States; email: lodmell@selway.umt.edu},\\r\\nissn={13558382},\\r\\ncoden={RNARF},\\r\\npubmed_id={12869707},\\r\\nlanguage={English},\\r\\nabbrev_source_title={RNA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Marzi, S.\",\"Knight, W.\",\"Brandi, L.\",\"Caserta, E.\",\"Soboleva, N.\",\"Hill, W.\",\"Gualerzi, C.\",\"Lodmell, J.\"],\"key\":\"Marzi2003958\",\"id\":\"Marzi2003958\",\"bibbaseid\":\"marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&doi=10.1261%2frna.2116303&partnerID=40&md5=b40491c4a5016c07d44c5c9308bbc767\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Konevega\"],\"firstnames\":[\"A.L.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenkov\"],\"firstnames\":[\"Yu.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the a Site of the 70S Ribosome\",\"journal\":\"Molecular Biology\",\"year\":\"2003\",\"volume\":\"37\",\"number\":\"1\",\"pages\":\"110-115\",\"doi\":\"10.1023/A:1022345115927\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&doi=10.1023%2fA%3a1022345115927&partnerID=40&md5=741954e32b6b93a44fe9c09521d9d1d0\",\"affiliation\":\"Konstantinov Inst. of Nucl. Physics, Russian Academy of Sciences, Gatchina, Leningrad Region, 188300, Russian Federation\",\"abstract\":\"To estimate the effect of modified nucleotide 37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA+YPhe and Phe-tRN A -YPhe) with the A site of complex [70S·poly(U) ·deacylated tRNAPhe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA+YPhe showed, removal of the Y base decreased the association constant of Phe-tRN A-Y Phe and the complex by an order of magnitude at every temperature tested, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNAPhe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRN A-YPhe but not for Phe-tRN A +YPhe. Thus, the modified nucleotide 3′ of the Phe-tRNAPhe anticodon stabilized the codon-anticodon interaction both in the A and P sites of the 70S ribosome.\",\"author_keywords\":\"70S ribosome; A site; Modified nucleotide; tRNAPhe; Y-base\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)01-04-49252\",\"key\":\"Soboleva2003110\",\"id\":\"Soboleva2003110\",\"bibbaseid\":\"anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&doi=10.1023%2fA%3a1022345115927&partnerID=40&md5=741954e32b6b93a44fe9c09521d9d1d0\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility\",\"journal\":\"Tsitologiya\",\"year\":\"2002\",\"volume\":\"44\",\"number\":\"5\",\"pages\":\"475-476\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&partnerID=40&md5=6cd83eb630a9b3ded1792a7091a98ebf\",\"affiliation\":\"St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation\",\"abstract\":\"On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.\",\"correspondence_address1\":\"Semenova, E.V.; St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation; email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova2002475,\\r\\nauthor={Semenova, E.V. and Drobchenko, E.A. and Filatov, M.V.},\\r\\ntitle={The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility},\\r\\njournal={Tsitologiya},\\r\\nyear={2002},\\r\\nvolume={44},\\r\\nnumber={5},\\r\\npages={475-476},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&partnerID=40&md5=6cd83eb630a9b3ded1792a7091a98ebf},\\r\\naffiliation={St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation},\\r\\nabstract={On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.},\\r\\ncorrespondence_address1={Semenova, E.V.; St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Drobchenko, E.\",\"Filatov, M.\"],\"key\":\"Semenova2002475\",\"id\":\"Semenova2002475\",\"bibbaseid\":\"semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&partnerID=40&md5=6cd83eb630a9b3ded1792a7091a98ebf\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Safronova\"],\"firstnames\":[\"I.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Maleic acid utilization by mixed cultures of microorganisms\",\"journal\":\"Applied Biochemistry and Microbiology\",\"year\":\"2002\",\"volume\":\"38\",\"number\":\"4\",\"pages\":\"346-348\",\"doi\":\"10.1023/A:1016283005773\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&doi=10.1023%2fA%3a1016283005773&partnerID=40&md5=688457cdf35a3f7a28492b01e8879a50\",\"affiliation\":\"Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation\",\"abstract\":\"In a mixed batch culture, Alcaligenes xylosoxidans subsp, xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and B. subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions of water.\",\"correspondence_address1\":\"Safronova, I.Yu.; Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru\",\"issn\":\"00036838\",\"language\":\"English\",\"abbrev_source_title\":\"Appl. Biochem. Microbiol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova2002346,\\r\\nauthor={Safronova, I.Yu. and Semenova, E.V.},\\r\\ntitle={Maleic acid utilization by mixed cultures of microorganisms},\\r\\njournal={Applied Biochemistry and Microbiology},\\r\\nyear={2002},\\r\\nvolume={38},\\r\\nnumber={4},\\r\\npages={346-348},\\r\\ndoi={10.1023/A:1016283005773},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&doi=10.1023%2fA%3a1016283005773&partnerID=40&md5=688457cdf35a3f7a28492b01e8879a50},\\r\\naffiliation={Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation},\\r\\nabstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp, xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and B. subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions of water.},\\r\\ncorrespondence_address1={Safronova, I.Yu.; Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},\\r\\nissn={00036838},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Appl. Biochem. Microbiol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Safronova, I.\",\"Semenova, E.\"],\"key\":\"Safronova2002346\",\"id\":\"Safronova2002346\",\"bibbaseid\":\"safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&doi=10.1023%2fA%3a1016283005773&partnerID=40&md5=688457cdf35a3f7a28492b01e8879a50\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sukhareva\"],\"firstnames\":[\"E.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shabalina\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing»\",\"journal\":\"Tsitologiya\",\"year\":\"2002\",\"volume\":\"44\",\"number\":\"6\",\"pages\":\"574-575\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&partnerID=40&md5=6a3d91e3788a2afd0731b1467fd0e5a9\",\"abstract\":\"The process of active dissociation of noncovalently bound agents from DNA or «DNA clearing» in the living cells was described elsewhere. The vital fluorescent bisbenzimidazole dye Hoechst 33342 (4342), which binds tightly but not covalently to DNA in the minor groove, was used for studying interactions of agents noncovalently binding with DNA. The «DNA clearing» is an energy-dependent process, which is suppressed by topoisomerase-II inhibitors and DNA breaks. It has been shown that the rodent fibroblast cell line AA8HoeR-7 is selected for resistance to H342, and characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. This cell line obtained cross-resistance to other DNA damaging drugs: mitomycin C, etoposide and ethidium bromide. That proves that AA8HoeR-7 is cell line with a new mechanism of multidrug resistance.\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"12236101\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Levina2002574,\\r\\nauthor={Levina, V.V. and Drobchenko, E.A. and Sukhareva, E.B. and Shabalina, E.V. and Filatov, M.V.},\\r\\ntitle={A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing»},\\r\\njournal={Tsitologiya},\\r\\nyear={2002},\\r\\nvolume={44},\\r\\nnumber={6},\\r\\npages={574-575},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&partnerID=40&md5=6a3d91e3788a2afd0731b1467fd0e5a9},\\r\\nabstract={The process of active dissociation of noncovalently bound agents from DNA or «DNA clearing» in the living cells was described elsewhere. The vital fluorescent bisbenzimidazole dye Hoechst 33342 (4342), which binds tightly but not covalently to DNA in the minor groove, was used for studying interactions of agents noncovalently binding with DNA. The «DNA clearing» is an energy-dependent process, which is suppressed by topoisomerase-II inhibitors and DNA breaks. It has been shown that the rodent fibroblast cell line AA8HoeR-7 is selected for resistance to H342, and characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. This cell line obtained cross-resistance to other DNA damaging drugs: mitomycin C, etoposide and ethidium bromide. That proves that AA8HoeR-7 is cell line with a new mechanism of multidrug resistance.},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={12236101},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Levina, V.\",\"Drobchenko, E.\",\"Sukhareva, E.\",\"Shabalina, E.\",\"Filatov, M.\"],\"key\":\"Levina2002574\",\"id\":\"Levina2002574\",\"bibbaseid\":\"levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&partnerID=40&md5=6a3d91e3788a2afd0731b1467fd0e5a9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Safronova\"],\"firstnames\":[\"I.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Maleic Acid Utilization by Mixed Cultures of Microorganisms\",\"journal\":\"Prikladnaya Biokhimiya i Mikrobiologiya\",\"year\":\"2002\",\"volume\":\"38\",\"number\":\"4\",\"pages\":\"403-404\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&partnerID=40&md5=fad79a8ef19fa76e2130fd1bdce1e62c\",\"affiliation\":\"Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation\",\"abstract\":\"In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.\",\"correspondence_address1\":\"Safronova, I.Yu.; Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"05551099\",\"pubmed_id\":\"12325296\",\"language\":\"Russian\",\"abbrev_source_title\":\"Prikl. Biokhim. Mikrobiol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova2002403,\\r\\nauthor={Safronova, I.Yu. and Semenova, E.V.},\\r\\ntitle={Maleic Acid Utilization by Mixed Cultures of Microorganisms},\\r\\njournal={Prikladnaya Biokhimiya i Mikrobiologiya},\\r\\nyear={2002},\\r\\nvolume={38},\\r\\nnumber={4},\\r\\npages={403-404},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&partnerID=40&md5=fad79a8ef19fa76e2130fd1bdce1e62c},\\r\\naffiliation={Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation},\\r\\nabstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.},\\r\\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={05551099},\\r\\npubmed_id={12325296},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Prikl. Biokhim. 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Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans transformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h-1. © 2001 MAIK \\\"Nauka/Interperiodica\\\".\",\"correspondence_address1\":\"Safronova, I.Yu.; Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation\",\"publisher\":\"Kluwer Academic/Plenum Publishers\",\"issn\":\"00036838\",\"language\":\"English\",\"abbrev_source_title\":\"Appl. Biochem. Microbiol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova2001374,\\r\\nauthor={Safronova, I.Yu. and Semenova, E.V.},\\r\\ntitle={Optimum conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260},\\r\\njournal={Applied Biochemistry and Microbiology},\\r\\nyear={2001},\\r\\nvolume={37},\\r\\nnumber={4},\\r\\npages={374-375},\\r\\ndoi={10.1023/A:1010245903543},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&doi=10.1023%2fA%3a1010245903543&partnerID=40&md5=a99a82099320d937b20e7b2a4432d13c},\\r\\naffiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\\r\\nabstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed 98% of the maleic acid (initial concentration of 5.0 g/l medium) under periodic conditions for 48 h. Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans transformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h-1. © 2001 MAIK \\\"Nauka/Interperiodica\\\".},\\r\\ncorrespondence_address1={Safronova, I.Yu.; Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\\r\\npublisher={Kluwer Academic/Plenum Publishers},\\r\\nissn={00036838},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Appl. Biochem. Microbiol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Safronova, I.\",\"Semenova, E.\"],\"key\":\"Safronova2001374\",\"id\":\"Safronova2001374\",\"bibbaseid\":\"safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&doi=10.1023%2fA%3a1010245903543&partnerID=40&md5=a99a82099320d937b20e7b2a4432d13c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Safronova\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.]\",\"journal\":\"Prikladnaia biokhimiia i mikrobiologiia\",\"year\":\"2001\",\"volume\":\"37\",\"number\":\"4\",\"pages\":\"436-438\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&partnerID=40&md5=9ce3e167f5fe2d61369252ced3a4fd9f\",\"affiliation\":\"Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation\",\"abstract\":\"Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.\",\"correspondence_address1\":\"Safronova, I.I.\",\"issn\":\"05551099\",\"pubmed_id\":\"11530667\",\"language\":\"Russian\",\"abbrev_source_title\":\"Prikl. Biokhim. Mikrobiol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova2001436,\\r\\nauthor={Safronova, I.I. and Semenova, E.V.},\\r\\ntitle={Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.]},\\r\\njournal={Prikladnaia biokhimiia i mikrobiologiia},\\r\\nyear={2001},\\r\\nvolume={37},\\r\\nnumber={4},\\r\\npages={436-438},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&partnerID=40&md5=9ce3e167f5fe2d61369252ced3a4fd9f},\\r\\naffiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\\r\\nabstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.},\\r\\ncorrespondence_address1={Safronova, I.I.},\\r\\nissn={05551099},\\r\\npubmed_id={11530667},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Prikl. Biokhim. Mikrobiol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Safronova, I.\",\"Semenova, E.\"],\"key\":\"Safronova2001436\",\"id\":\"Safronova2001436\",\"bibbaseid\":\"safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&partnerID=40&md5=9ce3e167f5fe2d61369252ced3a4fd9f\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Blizniukov\"],\"firstnames\":[\"O.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kozmin\"],\"firstnames\":[\"L.D.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Oliushin\"],\"firstnames\":[\"V.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ostreiko\"],\"firstnames\":[\"O.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral'noi nervnoi sistemy mogut byt' dostupny dlia ataki spetsificheskimi antitelami.]\",\"journal\":\"Voprosy onkologii\",\"year\":\"2001\",\"volume\":\"47\",\"number\":\"1\",\"pages\":\"49-51\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&partnerID=40&md5=9183e57df4f31e5ae6a0b50434f95a09\",\"affiliation\":\"St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation\",\"abstract\":\"The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.\",\"correspondence_address1\":\"Blizniukov, O.P.\",\"issn\":\"05073758\",\"pubmed_id\":\"11317535\",\"language\":\"Russian\",\"abbrev_source_title\":\"Vopr Onkol\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Blizniukov200149,\\r\\nauthor={Blizniukov, O.P. and Kozmin, L.D. and Oliushin, V.E. and Ostreiko, O.V. and Filatov, M.V.},\\r\\ntitle={Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral'noi nervnoi sistemy mogut byt' dostupny dlia ataki spetsificheskimi antitelami.]},\\r\\njournal={Voprosy onkologii},\\r\\nyear={2001},\\r\\nvolume={47},\\r\\nnumber={1},\\r\\npages={49-51},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&partnerID=40&md5=9183e57df4f31e5ae6a0b50434f95a09},\\r\\naffiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation},\\r\\nabstract={The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.},\\r\\ncorrespondence_address1={Blizniukov, O.P.},\\r\\nissn={05073758},\\r\\npubmed_id={11317535},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Vopr Onkol},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Blizniukov, O.\",\"Kozmin, L.\",\"Oliushin, V.\",\"Ostreiko, O.\",\"Filatov, M.\"],\"key\":\"Blizniukov200149\",\"id\":\"Blizniukov200149\",\"bibbaseid\":\"blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&partnerID=40&md5=9183e57df4f31e5ae6a0b50434f95a09\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lantsov\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting\",\"journal\":\"Doklady Akademii Nauk\",\"year\":\"2000\",\"volume\":\"371\",\"number\":\"6\",\"pages\":\"829-833\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&partnerID=40&md5=2af0b62eb2bdcdb6f93bb91fdd52b885\",\"issn\":\"08695652\",\"coden\":\"DAKNE\",\"language\":\"Russian\",\"abbrev_source_title\":\"Dokl. Akad. Nauk\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shcherbakova2000829,\\r\\nauthor={Shcherbakova, O.G. and Lantsov, V.A. and Filatov, M.V.},\\r\\ntitle={Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting},\\r\\njournal={Doklady Akademii Nauk},\\r\\nyear={2000},\\r\\nvolume={371},\\r\\nnumber={6},\\r\\npages={829-833},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&partnerID=40&md5=2af0b62eb2bdcdb6f93bb91fdd52b885},\\r\\nissn={08695652},\\r\\ncoden={DAKNE},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Dokl. Akad. Nauk},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shcherbakova, O.\",\"Lantsov, V.\",\"Filatov, M.\"],\"key\":\"Shcherbakova2000829\",\"id\":\"Shcherbakova2000829\",\"bibbaseid\":\"shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&partnerID=40&md5=2af0b62eb2bdcdb6f93bb91fdd52b885\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Aleshin\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tarakanov\"],\"firstnames\":[\"B.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Livshits\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]}],\"title\":\"A family öf shuttle vectors 'for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon\",\"journal\":\"Mikrobiologiya\",\"year\":\"2000\",\"volume\":\"69\",\"number\":\"1\",\"pages\":\"75-80\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&partnerID=40&md5=fa59c80a48a0c518643554b7cbd2df52\",\"affiliation\":\"All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation\",\"abstract\":\"A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in grampositive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc,) was created. The vectors are based on the cryptic plasmid pLFOll uom'Lactoimcillus fermentum VKM 1311 belonging to a family.of the o-type pE194-like plasmkls. The vectors can replicite in .gram-positive bacteria and Escherichia coll, \\\"They, are stable in many gram-positive bacteria, \\\"have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that'contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPot can' be mobilized in a great number' of bacteria using a' helper pltlmid from E, coll but not from1 gram-positive bacteria,.\",\"author_keywords\":\"Gram-positive bacteria; Lactic acid bacteria; Shuttle vectors; Ss-dna plasmids\",\"correspondence_address1\":\"Aleshin, V.V.; All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation\",\"issn\":\"00263656\",\"coden\":\"MIKBA\",\"pubmed_id\":\"10808493\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mikrobiologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Aleshin200075,\\r\\nauthor={Aleshin, V.V. and Semenova, E.V. and Tarakanov, B.V. and Livshits, V.A.},\\r\\ntitle={A family öf shuttle vectors 'for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon},\\r\\njournal={Mikrobiologiya},\\r\\nyear={2000},\\r\\nvolume={69},\\r\\nnumber={1},\\r\\npages={75-80},\\r\\nnote={cited By 3},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&partnerID=40&md5=fa59c80a48a0c518643554b7cbd2df52},\\r\\naffiliation={All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},\\r\\nabstract={A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in grampositive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc,) was created. The vectors are based on the cryptic plasmid pLFOll uom'Lactoimcillus fermentum VKM 1311 belonging to a family.of the o-type pE194-like plasmkls. The vectors can replicite in .gram-positive bacteria and Escherichia coll, \\\"They, are stable in many gram-positive bacteria, \\\"have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that'contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPot can' be mobilized in a great number' of bacteria using a' helper pltlmid from E, coll but not from1 gram-positive bacteria,.},\\r\\nauthor_keywords={Gram-positive bacteria;  Lactic acid bacteria;  Shuttle vectors;  Ss-dna plasmids},\\r\\ncorrespondence_address1={Aleshin, V.V.; All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},\\r\\nissn={00263656},\\r\\ncoden={MIKBA},\\r\\npubmed_id={10808493},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mikrobiologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Aleshin, V.\",\"Semenova, E.\",\"Tarakanov, B.\",\"Livshits, V.\"],\"key\":\"Aleshin200075\",\"id\":\"Aleshin200075\",\"bibbaseid\":\"aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&partnerID=40&md5=fa59c80a48a0c518643554b7cbd2df52\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Golikov\"],\"firstnames\":[\"P.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Davydov\"],\"firstnames\":[\"B.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Marchenko\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nikolaeva\"],\"firstnames\":[\"N.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Golikov\"],\"firstnames\":[\"A.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Riabinin\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Polumiskov\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]}],\"title\":\"Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel'nyi stress pri hypertonicheskikh krizakh.]\",\"journal\":\"Klinicheskaya Meditsina\",\"year\":\"2000\",\"volume\":\"78\",\"number\":\"7\",\"pages\":\"42-45\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&partnerID=40&md5=c8abc6251cfd7f665b4becca652d030a\",\"abstract\":\"Urapidil effects on oxidant stress were studied in 36 patients with hypertension stage I-III running with crises. Acute hypertensive crisis was managed by intravenous injections of urapidil (20-50 mg) for 5 min. After that for a week they received urapidil monotherapy followed by combined treatment for another week (quinapril–25 mg/day or ramipril–5-10 mg/day in combination with hypothiaside–25 mg/day; lokren–20 mg/day or atenolol–50-100 mg/day and diltiazem–180-360 mg/day) to stabilize arterial pressure finally. Oxidant stress was estimated by lipid peroxidation (LPO) and state of antioxidant system (AOS). Catabolism and anabolism were evaluated by blood levels of hydrocortisone and insulin. LPO, AOS, the blood hormones were measured before management of hypertensive crisis, immediately after it and 1, 3, 7 and 14 days after. Before the crisis treatment LPO was found high. Urapidil administration reduced levels of hydrocortisone, dienic conjugates, lipid peroxidation, oxidant stress rate (OSR). Later, LPO and OSR slowly increased. It is concluded on validity of the antioxidants use in hypertensive patients with crises.\",\"correspondence_address1\":\"Golikov, P.P.\",\"issn\":\"00232149\",\"pubmed_id\":\"10979642\",\"language\":\"Russian\",\"abbrev_source_title\":\"Klin Med (Mosk)\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Golikov200042,\\r\\nauthor={Golikov, P.P. and Davydov, B.V. and Marchenko, V.V. and Nikolaeva, N.I. and Golikov, A.P. and Riabinin, V.A. and Semenova, E.V. and Polumiskov, V.I.},\\r\\ntitle={Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel'nyi stress pri hypertonicheskikh krizakh.]},\\r\\njournal={Klinicheskaya Meditsina},\\r\\nyear={2000},\\r\\nvolume={78},\\r\\nnumber={7},\\r\\npages={42-45},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&partnerID=40&md5=c8abc6251cfd7f665b4becca652d030a},\\r\\nabstract={Urapidil effects on oxidant stress were studied in 36 patients with hypertension stage I-III running with crises. Acute hypertensive crisis was managed by intravenous injections of urapidil (20-50 mg) for 5 min. After that for a week they received urapidil monotherapy followed by combined treatment for another week (quinapril--25 mg/day or ramipril--5-10 mg/day in combination with hypothiaside--25 mg/day; lokren--20 mg/day or atenolol--50-100 mg/day and diltiazem--180-360 mg/day) to stabilize arterial pressure finally. Oxidant stress was estimated by lipid peroxidation (LPO) and state of antioxidant system (AOS). Catabolism and anabolism were evaluated by blood levels of hydrocortisone and insulin. LPO, AOS, the blood hormones were measured before management of hypertensive crisis, immediately after it and 1, 3, 7 and 14 days after. Before the crisis treatment LPO was found high. Urapidil administration reduced levels of hydrocortisone, dienic conjugates, lipid peroxidation, oxidant stress rate (OSR). Later, LPO and OSR slowly increased. It is concluded on validity of the antioxidants use in hypertensive patients with crises.},\\r\\ncorrespondence_address1={Golikov, P.P.},\\r\\nissn={00232149},\\r\\npubmed_id={10979642},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Klin Med (Mosk)},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Golikov, P.\",\"Davydov, B.\",\"Marchenko, V.\",\"Nikolaeva, N.\",\"Golikov, A.\",\"Riabinin, V.\",\"Semenova, E.\",\"Polumiskov, V.\"],\"key\":\"Golikov200042\",\"id\":\"Golikov200042\",\"bibbaseid\":\"golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&partnerID=40&md5=c8abc6251cfd7f665b4becca652d030a\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sukhareva\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shabalina\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.\"],\"suffixes\":[]}],\"title\":\"A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents\",\"journal\":\"ATLA Alternatives to Laboratory Animals\",\"year\":\"2000\",\"volume\":\"28\",\"number\":\"3\",\"pages\":\"451-456\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&partnerID=40&md5=70cc6ef737ebc7edab2a5f3f4dcc9536\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation\",\"abstract\":\"The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step- by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-1-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33342 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.\",\"author_keywords\":\"DNA clearing; Hoechst 33342; Mammalian cells; Mitomycin C; Multidrug resistance\",\"correspondence_address1\":\"Levina, V.; Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation\",\"issn\":\"02611929\",\"coden\":\"AALAD\",\"language\":\"English\",\"abbrev_source_title\":\"ATLA Altern. Lab. Anim.\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Levina2000451,\\r\\nauthor={Levina, V. and Varfolomeeva, E. and Sukhareva, E. and Shabalina, E. and Drobchenko, E. and Filatov, M.},\\r\\ntitle={A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents},\\r\\njournal={ATLA Alternatives to Laboratory Animals},\\r\\nyear={2000},\\r\\nvolume={28},\\r\\nnumber={3},\\r\\npages={451-456},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&partnerID=40&md5=70cc6ef737ebc7edab2a5f3f4dcc9536},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},\\r\\nabstract={The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step- by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-1-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33342 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.},\\r\\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Mitomycin C;  Multidrug resistance},\\r\\ncorrespondence_address1={Levina, V.; Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},\\r\\nissn={02611929},\\r\\ncoden={AALAD},\\r\\nlanguage={English},\\r\\nabbrev_source_title={ATLA Altern. Lab. Anim.},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Levina, V.\",\"Varfolomeeva, E.\",\"Sukhareva, E.\",\"Shabalina, E.\",\"Drobchenko, E.\",\"Filatov, M.\"],\"key\":\"Levina2000451\",\"id\":\"Levina2000451\",\"bibbaseid\":\"levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&partnerID=40&md5=70cc6ef737ebc7edab2a5f3f4dcc9536\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lanzov\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ogawa\"],\"firstnames\":[\"H.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells\",\"journal\":\"Mutation Research - DNA Repair\",\"year\":\"2000\",\"volume\":\"459\",\"number\":\"1\",\"pages\":\"65-71\",\"doi\":\"10.1016/S0921-8777(99)00059-2\",\"note\":\"cited By 30\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&doi=10.1016%2fS0921-8777%2899%2900059-2&partnerID=40&md5=e702eb119dd51b6527762dbac2a88b00\",\"affiliation\":\"Molec. and Radiat. Biophys. Division, Petersburg Nucl. Phys. Inst., 188350, Gatchina, Russian Federation; Department of Biology, Osaka University, Osaka, Japan\",\"abstract\":\"The pairing of homologous molecules and strand exchange is a key event in homologous recombination promoted by RecA protein in Escherichia coli. Structural homologs of RecA are widely distributed in eukaryotes including mouse and man. As has been shown, human HsRad51 protein is not only structural but also functional homolog of RecA. The question arises whether the bacterial functional homolog of Rad51 can function in mammalian cells and increase the frequency of the homologous recombination. To investigate possible effects of bacterial RecA protein on the frequency of homologous recombination in mammalian cells, the E. coli RecA protein fused with a nuclear location signal from the large T antigen of simian virus 40 was overexpressed in the mouse F9 teratocarcinoma cells. We found that the frequency of gene targeting at the hprt locus was 10-fold increased in the mouse cells expressing the nucleus-targeted RecA protein. Southern blot analysis of individual clones that were generated by targeting recombination revealed predicted type of alterations in hprt gene. The data indicate that the bacterial nucleus-targeted RecA protein can stimulate homologous recombination in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.\",\"author_keywords\":\"Gene targeting; Homologous recombination in somatic mammalian cells; RecA protein\",\"funding_details\":\"Howard Hughes Medical Institute75195-546101\",\"key\":\"Shcherbakova200065\",\"id\":\"Shcherbakova200065\",\"bibbaseid\":\"anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&doi=10.1016%2fS0921-8777%2899%2900059-2&partnerID=40&md5=e702eb119dd51b6527762dbac2a88b00\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells\",\"journal\":\"Biochimica et Biophysica Acta - Molecular Cell Research\",\"year\":\"2000\",\"volume\":\"1495\",\"number\":\"1\",\"pages\":\"1-3\",\"doi\":\"10.1016/S0167-4889(99)00151-2\",\"note\":\"cited By 10\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&doi=10.1016%2fS0167-4889%2899%2900151-2&partnerID=40&md5=da5b2e1f4286e2c7e96d04eac53c4b64\",\"affiliation\":\"Molec. and Radiat. Biophys. Division, Petersburg Nucl. Phys. Inst., 188350, Gatchina, Russian Federation\",\"abstract\":\"In order to study the involvement of DNA topoisomerase I (top1) in recombination, we examined the effect of the anti-neoplastic drug camptothecin, which selectively poisons top1 by trapping top1-cleavable complexes on integration of exogenic vector into the genome of mammalian cells. We transfected mouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a plasmid carrying a selectable neo gene treated with camptothecin, and determined the frequency of neo+ (G418(R)) colonies. We found that treatment with camptothecin for as short a time as 4 h after electroporation resulted in a 4- to 33-fold stimulation of plasmid integration into the recipient genome via non-homologous recombination. These results imply that top1-cleavable complexes trapped by camptothecin could be potentially recombinogenic structures and could stimulate non-homologous recombination in vivo, promoting the integration of transfected plasmids into mammalian genome. Copyright (C) 2000 Elsevier Science B.V.\",\"author_keywords\":\"Camptothecin; Mammalian somatic cell; Non-homologous recombination; Topoisomerase 1; Transfected DNA\",\"correspondence_address1\":\"Shcherbakova, O.G.; Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, 188350 Gatchina, Russian Federation; email: olia@omrb.pnpi.spb.ru\",\"issn\":\"01674889\",\"coden\":\"BAMRD\",\"pubmed_id\":\"10634926\",\"language\":\"English\",\"abbrev_source_title\":\"Biochim. Biophys. Acta Mol. Cell Res.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shcherbakova20001,\\r\\nauthor={Shcherbakova, O.G. and Filatov, M.V.},\\r\\ntitle={Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells},\\r\\njournal={Biochimica et Biophysica Acta - Molecular Cell Research},\\r\\nyear={2000},\\r\\nvolume={1495},\\r\\nnumber={1},\\r\\npages={1-3},\\r\\ndoi={10.1016/S0167-4889(99)00151-2},\\r\\nnote={cited By 10},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&doi=10.1016%2fS0167-4889%2899%2900151-2&partnerID=40&md5=da5b2e1f4286e2c7e96d04eac53c4b64},\\r\\naffiliation={Molec. and Radiat. Biophys. Division, Petersburg Nucl. Phys. Inst., 188350, Gatchina, Russian Federation},\\r\\nabstract={In order to study the involvement of DNA topoisomerase I (top1) in recombination, we examined the effect of the anti-neoplastic drug camptothecin, which selectively poisons top1 by trapping top1-cleavable complexes on integration of exogenic vector into the genome of mammalian cells. We transfected mouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a plasmid carrying a selectable neo gene treated with camptothecin, and determined the frequency of neo+ (G418(R)) colonies. We found that treatment with camptothecin for as short a time as 4 h after electroporation resulted in a 4- to 33-fold stimulation of plasmid integration into the recipient genome via non-homologous recombination. These results imply that top1-cleavable complexes trapped by camptothecin could be potentially recombinogenic structures and could stimulate non-homologous recombination in vivo, promoting the integration of transfected plasmids into mammalian genome. Copyright (C) 2000 Elsevier Science B.V.},\\r\\nauthor_keywords={Camptothecin;  Mammalian somatic cell;  Non-homologous recombination;  Topoisomerase 1;  Transfected DNA},\\r\\ncorrespondence_address1={Shcherbakova, O.G.; Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, 188350 Gatchina, Russian Federation; email: olia@omrb.pnpi.spb.ru},\\r\\nissn={01674889},\\r\\ncoden={BAMRD},\\r\\npubmed_id={10634926},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Biochim. Biophys. Acta Mol. Cell Res.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shcherbakova, O.\",\"Filatov, M.\"],\"key\":\"Shcherbakova20001\",\"id\":\"Shcherbakova20001\",\"bibbaseid\":\"shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&doi=10.1016%2fS0167-4889%2899%2900151-2&partnerID=40&md5=da5b2e1f4286e2c7e96d04eac53c4b64\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Scherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lanzov\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting.\",\"journal\":\"Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry section / translated from Russian\",\"year\":\"2000\",\"volume\":\"371\",\"number\":\"1-6\",\"pages\":\"69-72\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&partnerID=40&md5=6d2b5ab3cc3a34ddf31edb4d0a59e434\",\"affiliation\":\"Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"correspondence_address1\":\"Scherbakova, O.G.\",\"issn\":\"00124958\",\"pubmed_id\":\"10853454\",\"language\":\"English\",\"abbrev_source_title\":\"Dokl. Biochem.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Scherbakova200069,\\r\\nauthor={Scherbakova, O.G. and Lanzov, V.A. and Filatov, M.V.},\\r\\ntitle={Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting.},\\r\\njournal={Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry section / translated from Russian},\\r\\nyear={2000},\\r\\nvolume={371},\\r\\nnumber={1-6},\\r\\npages={69-72},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&partnerID=40&md5=6d2b5ab3cc3a34ddf31edb4d0a59e434},\\r\\naffiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\ncorrespondence_address1={Scherbakova, O.G.},\\r\\nissn={00124958},\\r\\npubmed_id={10853454},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Dokl. Biochem.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Scherbakova, O.\",\"Lanzov, V.\",\"Filatov, M.\"],\"key\":\"Scherbakova200069\",\"id\":\"Scherbakova200069\",\"bibbaseid\":\"scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&partnerID=40&md5=6d2b5ab3cc3a34ddf31edb4d0a59e434\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Aleshin\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tarakanov\"],\"firstnames\":[\"B.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Livshits\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]}],\"title\":\"A family of shuttle vectors for lactic acid bacteria and other gram-positive bacteria based on the plasmid pLF1311 replicon\",\"journal\":\"Microbiology\",\"year\":\"2000\",\"volume\":\"69\",\"number\":\"1\",\"pages\":\"63-67\",\"doi\":\"10.1007/BF02757259\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&doi=10.1007%2fBF02757259&partnerID=40&md5=741ca6fb3cfdb09a5156ff022f6829b1\",\"affiliation\":\"All-Russia Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation; Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi Proezd 1, Moscow 113545, Russian Federation\",\"abstract\":\"A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. The vectors are based on the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM 1311, belonging to the family of the σ-type pE194-like plasmids. The vectors can replicate in gram-positive bacteria and Escherichia coli. They are stable in many gram-positive bacteria, have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPα can be mobilized in a great number of bacteria using a helper plasmid from E. coli but not from gram-positive bacteria. © 2000 MAIK \\\"Nauka/Interperiodica\\\".\",\"author_keywords\":\"Gram-positive bacteria; Lactic acid bacteria; Shuttle vectors; ss-DNA plasmids\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)96-04-49239\",\"key\":\"Aleshin200063\",\"id\":\"Aleshin200063\",\"bibbaseid\":\"anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&doi=10.1007%2fBF02757259&partnerID=40&md5=741ca6fb3cfdb09a5156ff022f6829b1\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shevelev\"],\"firstnames\":[\"A.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Aleoshin\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Trachuk\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Granovsky\"],\"firstnames\":[\"A.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kogan\"],\"firstnames\":[\"Y.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Rumer\"],\"firstnames\":[\"L.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Serkina\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bushueva\"],\"firstnames\":[\"A.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Livshits\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kostrov\"],\"firstnames\":[\"S.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcheglov\"],\"firstnames\":[\"A.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Novikova\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Chestukhina\"],\"firstnames\":[\"G.G.\"],\"suffixes\":[]}],\"title\":\"Expression of bacillar glutamyl endopeptidase genes in Bacillus subtilis by a new mobilizable single-replicon vector pLF\",\"journal\":\"Plasmid\",\"year\":\"2000\",\"volume\":\"43\",\"number\":\"3\",\"pages\":\"190-199\",\"doi\":\"10.1006/plas.1999.1455\",\"note\":\"cited By 5\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&doi=10.1006%2fplas.1999.1455&partnerID=40&md5=86a151c76a9bd0d6c8f657503f14b257\",\"affiliation\":\"Inst. Genet. Sel. Indust. M., Moscow, Russian Federation; Inst. Physiological, Biochem., N., Borovsk, Kaluzhskaya Region, Russian Federation; Institute of Molecular Genetics, Moscow, Russian Federation; Moscow Inst. Phys. Technol. - T., Moscow, Russian Federation\",\"abstract\":\"The pLF1311 natural plasmid from Lactobacillus fermentum 1311 was used to construct a single replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and Escherichia coli. The new vector is capable of conjugative mobilization from E. coli to various hosts by conjugal transfer. The final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. Glutamyl endopeptidase genes from Bacillus licheniformis (gseBL) and B. intermedius (gseB1) were cloned in both pLF9 and pLF14 vectors and introduced to B. subtilis. The yield of enzymes in the pLF-derived producers was 6- to 30-fold more than in the natural producers and reached 100-150 mg/L of mature protease. (C) 2000 Academic Press.\",\"author_keywords\":\"B. intermedius; B. licheniformis; Bacillus subtilis; Glutamyl endopeptidase; Mating; Secretion\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)98-04-48168, 97-04-50162\",\"key\":\"Shevelev2000190\",\"id\":\"Shevelev2000190\",\"bibbaseid\":\"anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&doi=10.1006%2fplas.1999.1455&partnerID=40&md5=86a151c76a9bd0d6c8f657503f14b257\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tret'yakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells\",\"journal\":\"Doklady Akademii Nauk\",\"year\":\"1999\",\"volume\":\"364\",\"number\":\"3\",\"pages\":\"402-404\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&partnerID=40&md5=b75439e5584ecc0bb4557fa9b5830fdf\",\"issn\":\"08695652\",\"coden\":\"DAKNE\",\"pubmed_id\":\"10188092\",\"language\":\"English; Russian\",\"abbrev_source_title\":\"Dokl. Akad. Nauk\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Varfolomeeva1999402,\\r\\nauthor={Varfolomeeva, E.Yu. and Levina, V.V. and Tret'yakov, A.N. and Drobchenko, E.A. and Filatov, M.V.},\\r\\ntitle={Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells},\\r\\njournal={Doklady Akademii Nauk},\\r\\nyear={1999},\\r\\nvolume={364},\\r\\nnumber={3},\\r\\npages={402-404},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&partnerID=40&md5=b75439e5584ecc0bb4557fa9b5830fdf},\\r\\nissn={08695652},\\r\\ncoden={DAKNE},\\r\\npubmed_id={10188092},\\r\\nlanguage={English; Russian},\\r\\nabbrev_source_title={Dokl. Akad. Nauk},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Varfolomeeva, E.\",\"Levina, V.\",\"Tret'yakov, A.\",\"Drobchenko, E.\",\"Filatov, M.\"],\"key\":\"Varfolomeeva1999402\",\"id\":\"Varfolomeeva1999402\",\"bibbaseid\":\"varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&partnerID=40&md5=b75439e5584ecc0bb4557fa9b5830fdf\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kaboev\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shevelev\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Luchkina\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tret'yakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]}],\"title\":\"Hot start of the polymerase chain reaction using DNA helicases\",\"journal\":\"Bioorganicheskaya Khimiya\",\"year\":\"1999\",\"volume\":\"25\",\"number\":\"5\",\"pages\":\"400\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&partnerID=40&md5=f0d90567c33a31e593c4e7a9fbbcaedf\",\"affiliation\":\"Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation\",\"abstract\":\"A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.\",\"author_keywords\":\"DNA helicase; Hot start; PCR\",\"correspondence_address1\":\"Kaboev, O.K.; Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.spb.ru\",\"issn\":\"01323423\",\"language\":\"Russian\",\"abbrev_source_title\":\"Bioorganicheskaya Khim.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kaboev1999400,\\r\\nauthor={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret'yakov, A.N. and Shcherbakova, O.G.},\\r\\ntitle={Hot start of the polymerase chain reaction using DNA helicases},\\r\\njournal={Bioorganicheskaya Khimiya},\\r\\nyear={1999},\\r\\nvolume={25},\\r\\nnumber={5},\\r\\npages={400},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&partnerID=40&md5=f0d90567c33a31e593c4e7a9fbbcaedf},\\r\\naffiliation={Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\\r\\nabstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.},\\r\\nauthor_keywords={DNA helicase;  Hot start;  PCR},\\r\\ncorrespondence_address1={Kaboev, O.K.; Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.spb.ru},\\r\\nissn={01323423},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Bioorganicheskaya Khim.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kaboev, O.\",\"Shevelev, I.\",\"Luchkina, L.\",\"Tret'yakov, A.\",\"Shcherbakova, O.\"],\"key\":\"Kaboev1999400\",\"id\":\"Kaboev1999400\",\"bibbaseid\":\"kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&partnerID=40&md5=f0d90567c33a31e593c4e7a9fbbcaedf\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Savel-1\",\"id\":\"Savel-1\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vorob'eva\"],\"firstnames\":[\"O.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Leont'eva\"],\"firstnames\":[\"O.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]}],\"title\":\"Relationship between abnormal sperm chromatin packing and IVF results\",\"journal\":\"Molecular Human Reproduction\",\"year\":\"1999\",\"volume\":\"5\",\"number\":\"9\",\"pages\":\"825-830\",\"doi\":\"10.1093/molehr/5.9.825\",\"note\":\"cited By 62\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&doi=10.1093%2fmolehr%2f5.9.825&partnerID=40&md5=749736b469111d208fd9a86f6f59e531\",\"affiliation\":\"Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation\",\"abstract\":\"This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross- linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.\",\"author_keywords\":\"Chromatin packing; Ethidium bromide; Fertility disorder; Flow cytometry; Human spermatozoa\",\"correspondence_address1\":\"Filatov, M.V.; Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation\",\"issn\":\"13609947\",\"coden\":\"MHREF\",\"pubmed_id\":\"10460220\",\"language\":\"English\",\"abbrev_source_title\":\"Mol. Hum. Reprod.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1999825,\\r\\nauthor={Filatov, M.V. and Semenova, E.V. and Vorob'eva, O.A. and Leont'eva, O.A. and Drobchenko, E.A.},\\r\\ntitle={Relationship between abnormal sperm chromatin packing and IVF results},\\r\\njournal={Molecular Human Reproduction},\\r\\nyear={1999},\\r\\nvolume={5},\\r\\nnumber={9},\\r\\npages={825-830},\\r\\ndoi={10.1093/molehr/5.9.825},\\r\\nnote={cited By 62},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&doi=10.1093%2fmolehr%2f5.9.825&partnerID=40&md5=749736b469111d208fd9a86f6f59e531},\\r\\naffiliation={Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},\\r\\nabstract={This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross- linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.},\\r\\nauthor_keywords={Chromatin packing;  Ethidium bromide;  Fertility disorder;  Flow cytometry;  Human spermatozoa},\\r\\ncorrespondence_address1={Filatov, M.V.; Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},\\r\\nissn={13609947},\\r\\ncoden={MHREF},\\r\\npubmed_id={10460220},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mol. Hum. Reprod.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Semenova, E.\",\"Vorob'eva, O.\",\"Leont'eva, O.\",\"Drobchenko, E.\"],\"key\":\"Filatov1999825\",\"id\":\"Filatov1999825\",\"bibbaseid\":\"filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&doi=10.1093%2fmolehr%2f5.9.825&partnerID=40&md5=749736b469111d208fd9a86f6f59e531\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Aleshin\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Doroshenko\"],\"firstnames\":[\"V.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Jomantas\"],\"firstnames\":[\"Y.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tarakanov\"],\"firstnames\":[\"B.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Livshits\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]}],\"title\":\"The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311\",\"journal\":\"FEMS Microbiology Letters\",\"year\":\"1999\",\"volume\":\"178\",\"number\":\"1\",\"pages\":\"47-53\",\"doi\":\"10.1016/S0378-1097(99)00343-2\",\"note\":\"cited By 12\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&doi=10.1016%2fS0378-1097%2899%2900343-2&partnerID=40&md5=dc537540b7e1b1d040043daddc06b0e6\",\"affiliation\":\"State Res. Inst. Genet. Sel. I., 1 Dorozhny proezd, 1, Moscow 113545, Russian Federation; Res. Inst. Physiol., Biochem. N., Russ. Acad. of Agricultural Sciences, Borovsk 249010, Russian Federation\",\"abstract\":\"The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram-positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non-selective conditions. Copyright (C) 1999 Federation of European Microbiological Societies.\",\"author_keywords\":\"Lactobacillus plasmid; Mobilizable vector; Rolling circle replication\",\"funding_details\":\"Российский Фонд Фундаментальных Исследований (РФФИ)97-04-48818, 96-04-49239\",\"key\":\"Aleshin199947\",\"id\":\"Aleshin199947\",\"bibbaseid\":\"anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&doi=10.1016%2fS0378-1097%2899%2900343-2&partnerID=40&md5=dc537540b7e1b1d040043daddc06b0e6\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Naryzhny\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?\",\"journal\":\"Electrophoresis\",\"year\":\"1999\",\"volume\":\"20\",\"number\":\"4-5\",\"pages\":\"1033-1038\",\"doi\":\"10.1002/(SICI)1522-2683(19990101)20:4/5<1033::AID-ELPS1033>3.0.CO;2-3\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&partnerID=40&md5=3f6b63ef15d5a83f4e04cddb3b81eaeb\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, 188350, Russian Federation\",\"abstract\":\"It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA.\",\"author_keywords\":\"DNA clearing; Hoechst 33342; Mammalian cells; Two-dimensional polyacrylamide gel electrophoresis\",\"correspondence_address1\":\"Naryzhny, S.N.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad District 188350, Russian Federation; email: naryzhny@omrb.pnpi.spb.ru\",\"issn\":\"01730835\",\"coden\":\"ELCTD\",\"pubmed_id\":\"10344282\",\"language\":\"English\",\"abbrev_source_title\":\"Electrophoresis\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Naryzhny19991033,\\r\\nauthor={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},\\r\\ntitle={Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?},\\r\\njournal={Electrophoresis},\\r\\nyear={1999},\\r\\nvolume={20},\\r\\nnumber={4-5},\\r\\npages={1033-1038},\\r\\ndoi={10.1002/(SICI)1522-2683(19990101)20:4/5<1033::AID-ELPS1033>3.0.CO;2-3},\\r\\nnote={cited By 3},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&partnerID=40&md5=3f6b63ef15d5a83f4e04cddb3b81eaeb},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, 188350, Russian Federation},\\r\\nabstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA.},\\r\\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional polyacrylamide gel electrophoresis},\\r\\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad District 188350, Russian Federation; email: naryzhny@omrb.pnpi.spb.ru},\\r\\nissn={01730835},\\r\\ncoden={ELCTD},\\r\\npubmed_id={10344282},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Electrophoresis},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Naryzhny, S.\",\"Levina, V.\",\"Varfolomeeva, E.\",\"Drobchenko, E.\",\"Filatov, M.\"],\"key\":\"Naryzhny19991033\",\"id\":\"Naryzhny19991033\",\"bibbaseid\":\"naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&partnerID=40&md5=3f6b63ef15d5a83f4e04cddb3b81eaeb\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kaboev\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shevelev\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Luchkina\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tret'yakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]}],\"title\":\"Hot start of the polymerase chain reaction using DNA helicases\",\"journal\":\"Russian Journal of Bioorganic Chemistry\",\"year\":\"1999\",\"volume\":\"25\",\"number\":\"5\",\"pages\":\"350-351\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&partnerID=40&md5=72d82556ef27e324b46c8a4c6b27a4b9\",\"affiliation\":\"St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation\",\"abstract\":\"A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture. © 1999 MAEe cyrillic signK \\\"Hayκa/Interperiodica\\\".\",\"author_keywords\":\"DNA helicase; Hot start; PCR\",\"correspondence_address1\":\"Kaboev, O.K.; St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.sbr.ru\",\"issn\":\"10681620\",\"language\":\"English\",\"abbrev_source_title\":\"Russ. J. Bioorg. Chem.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Kaboev1999350,\\r\\nauthor={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret'yakov, A.N. and Shcherbakova, O.G.},\\r\\ntitle={Hot start of the polymerase chain reaction using DNA helicases},\\r\\njournal={Russian Journal of Bioorganic Chemistry},\\r\\nyear={1999},\\r\\nvolume={25},\\r\\nnumber={5},\\r\\npages={350-351},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&partnerID=40&md5=72d82556ef27e324b46c8a4c6b27a4b9},\\r\\naffiliation={St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\\r\\nabstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture. © 1999 MAEe cyrillic signK \\\"Hayκa/Interperiodica\\\".},\\r\\nauthor_keywords={DNA helicase;  Hot start;  PCR},\\r\\ncorrespondence_address1={Kaboev, O.K.; St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.sbr.ru},\\r\\nissn={10681620},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Russ. J. Bioorg. Chem.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Kaboev, O.\",\"Shevelev, I.\",\"Luchkina, L.\",\"Tret'yakov, A.\",\"Shcherbakova, O.\"],\"key\":\"Kaboev1999350\",\"id\":\"Kaboev1999350\",\"bibbaseid\":\"kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&partnerID=40&md5=72d82556ef27e324b46c8a4c6b27a4b9\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Smirnova\"],\"firstnames\":[\"T.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells\",\"journal\":\"Tsitologiya\",\"year\":\"1999\",\"volume\":\"41\",\"number\":\"11\",\"pages\":\"951\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&partnerID=40&md5=ff82ecf873c4549e7c93f8fae7ce5775\",\"abstract\":\"A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system.\",\"author_keywords\":\"Gene targeting; hprt; Illegitimate recombination; Poly(ADP-ribose) polymerase; Topoisomerases\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"10643051\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Shcherbakova1999951,\\r\\nauthor={Shcherbakova, O.G. and Smirnova, T.V. and Filatov, M.V.},\\r\\ntitle={Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells},\\r\\njournal={Tsitologiya},\\r\\nyear={1999},\\r\\nvolume={41},\\r\\nnumber={11},\\r\\npages={951},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&partnerID=40&md5=ff82ecf873c4549e7c93f8fae7ce5775},\\r\\nabstract={A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system.},\\r\\nauthor_keywords={Gene targeting;  hprt;  Illegitimate recombination;  Poly(ADP-ribose) polymerase;  Topoisomerases},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={10643051},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Shcherbakova, O.\",\"Smirnova, T.\",\"Filatov, M.\"],\"key\":\"Shcherbakova1999951\",\"id\":\"Shcherbakova1999951\",\"bibbaseid\":\"shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&partnerID=40&md5=ff82ecf873c4549e7c93f8fae7ce5775\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ivanov\"],\"firstnames\":[\"E.\"],\"suffixes\":[]}],\"title\":\"Vitamin E: blocking the beginnings of atherosclerosis?\",\"journal\":\"Molecular medicine today\",\"year\":\"1999\",\"volume\":\"5\",\"number\":\"1\",\"pages\":\"8\",\"doi\":\"10.1016/S1357-4310(98)01402-6\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&doi=10.1016%2fS1357-4310%2898%2901402-6&partnerID=40&md5=038c62d4e6a2ed7c1c6f416237e01f51\",\"correspondence_address1\":\"Ivanov, E.email: eivanov@aol.com\",\"issn\":\"13574310\",\"pubmed_id\":\"10088123\",\"language\":\"English\",\"abbrev_source_title\":\"Mol Med Today\",\"document_type\":\"Short Survey\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ivanov19998,\\r\\nauthor={Ivanov, E.},\\r\\ntitle={Vitamin E: blocking the beginnings of atherosclerosis?},\\r\\njournal={Molecular medicine today},\\r\\nyear={1999},\\r\\nvolume={5},\\r\\nnumber={1},\\r\\npages={8},\\r\\ndoi={10.1016/S1357-4310(98)01402-6},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&doi=10.1016%2fS1357-4310%2898%2901402-6&partnerID=40&md5=038c62d4e6a2ed7c1c6f416237e01f51},\\r\\ncorrespondence_address1={Ivanov, E.email: eivanov@aol.com},\\r\\nissn={13574310},\\r\\npubmed_id={10088123},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mol Med Today},\\r\\ndocument_type={Short Survey},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ivanov, E.\"],\"key\":\"Ivanov19998\",\"id\":\"Ivanov19998\",\"bibbaseid\":\"ivanov-vitamineblockingthebeginningsofatherosclerosis-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&doi=10.1016%2fS1357-4310%2898%2901402-6&partnerID=40&md5=038c62d4e6a2ed7c1c6f416237e01f51\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Tarasov\"],\"firstnames\":[\"V.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kisliakova\"],\"firstnames\":[\"T.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskov\"],\"firstnames\":[\"F.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Koloskov\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stavrovietski\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Onikienko\"],\"firstnames\":[\"S.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kletchikov\"],\"firstnames\":[\"V.Z.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Lvov\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Blizniukov\"],\"firstnames\":[\"O.P.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kiselevski\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer\",\"journal\":\"Hybridoma\",\"year\":\"1999\",\"volume\":\"18\",\"number\":\"1\",\"pages\":\"99-102\",\"doi\":\"10.1089/hyb.1999.18.99\",\"note\":\"cited By 5\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&doi=10.1089%2fhyb.1999.18.99&partnerID=40&md5=2b2d61bdf666822de71b5967cb78bb5c\",\"affiliation\":\"Department of Thorax Surgery, Med. Acad. of Postgraduated Educ., 196247, St. Petersburg, Russian Federation; City Hospital No. 26, 196247, St. Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, St. Petersburg, 188350, Russian Federation; Petersburg Nuclear Physics Institute, 188350, Gatchina, St. Petersburg, Russian Federation\",\"abstract\":\"Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilie de Calmette-Guerin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.\",\"correspondence_address1\":\"Filatov, M.V.; Petersburg Nuclear Physics Institute, 188350 Gatchina, St. Petersburg, Russian Federation\",\"publisher\":\"Mary Ann Liebert Inc.\",\"issn\":\"0272457X\",\"coden\":\"HYBRD\",\"pubmed_id\":\"10211796\",\"language\":\"English\",\"abbrev_source_title\":\"Hybridoma\",\"document_type\":\"Conference Paper\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Tarasov199999,\\r\\nauthor={Tarasov, V.A. and Filatov, M.V. and Kisliakova, T.V. and Noskov, F.S. and Koloskov, A.V. and Stavrovietski, V.V. and Onikienko, S.B. and Kletchikov, V.Z. and Lvov, I.V. and Varfolomeeva, E.Yu. and Blizniukov, O.P. and Levina, V.V. and Kiselevski, M.V.},\\r\\ntitle={Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer},\\r\\njournal={Hybridoma},\\r\\nyear={1999},\\r\\nvolume={18},\\r\\nnumber={1},\\r\\npages={99-102},\\r\\ndoi={10.1089/hyb.1999.18.99},\\r\\nnote={cited By 5},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&doi=10.1089%2fhyb.1999.18.99&partnerID=40&md5=2b2d61bdf666822de71b5967cb78bb5c},\\r\\naffiliation={Department of Thorax Surgery, Med. Acad. of Postgraduated Educ., 196247, St. Petersburg, Russian Federation; City Hospital No. 26, 196247, St. Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, St. Petersburg, 188350, Russian Federation; Petersburg Nuclear Physics Institute, 188350, Gatchina, St. Petersburg, Russian Federation},\\r\\nabstract={Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilie de Calmette-Guerin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.},\\r\\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, 188350 Gatchina, St. Petersburg, Russian Federation},\\r\\npublisher={Mary Ann Liebert Inc.},\\r\\nissn={0272457X},\\r\\ncoden={HYBRD},\\r\\npubmed_id={10211796},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Hybridoma},\\r\\ndocument_type={Conference Paper},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Tarasov, V.\",\"Filatov, M.\",\"Kisliakova, T.\",\"Noskov, F.\",\"Koloskov, A.\",\"Stavrovietski, V.\",\"Onikienko, S.\",\"Kletchikov, V.\",\"Lvov, I.\",\"Varfolomeeva, E.\",\"Blizniukov, O.\",\"Levina, V.\",\"Kiselevski, M.\"],\"key\":\"Tarasov199999\",\"id\":\"Tarasov199999\",\"bibbaseid\":\"tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&doi=10.1089%2fhyb.1999.18.99&partnerID=40&md5=2b2d61bdf666822de71b5967cb78bb5c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"\\\"DNA clearing\\\" from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance\",\"journal\":\"Biologicheskie Membrany\",\"year\":\"1998\",\"volume\":\"15\",\"number\":\"6\",\"pages\":\"706-707\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&partnerID=40&md5=a98a72c5e0367996d29f6a7ca63e9233\",\"affiliation\":\"St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"correspondence_address1\":\"Levina, V.V.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"issn\":\"02334755\",\"language\":\"Russian\",\"abbrev_source_title\":\"Biol. Membr.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Levina1998706,\\r\\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},\\r\\ntitle={\\\"DNA clearing\\\" from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance},\\r\\njournal={Biologicheskie Membrany},\\r\\nyear={1998},\\r\\nvolume={15},\\r\\nnumber={6},\\r\\npages={706-707},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&partnerID=40&md5=a98a72c5e0367996d29f6a7ca63e9233},\\r\\naffiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\ncorrespondence_address1={Levina, V.V.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\nissn={02334755},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Biol. Membr.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Levina, V.\",\"Varfolomeeva, E.\",\"Filatov, M.\"],\"key\":\"Levina1998706\",\"id\":\"Levina1998706\",\"bibbaseid\":\"levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&partnerID=40&md5=a98a72c5e0367996d29f6a7ca63e9233\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"'DNA clearing' from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance\",\"journal\":\"Membrane and Cell Biology\",\"year\":\"1998\",\"volume\":\"12\",\"number\":\"6\",\"pages\":\"883-893\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&partnerID=40&md5=693d0527f04b69d04f82f18f75215244\",\"affiliation\":\"St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation\",\"abstract\":\"Earlier, we have described the process of active dissociation or 'DNA clearing' from non-covalently bound agents in living mammalian cells. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds DNA in the minor groove tightly but non-covalently, was used for studying the interaction of non-covalently binding agents with DNA. Multiple drug resistance (MDR) in tumour cells is related to the expression of transport proteins that alter the cellular drug transport and distribution. Three different groups of genes (mdr, MRP, and LRP) and their products are implicated in MDR. To obtain new cell lines characterized by enhanced process of active dissociation of non-covalently bound agents from DNA or 'DNA clearing', we carried out step-by-step selection with increasing concentrations of Hoechst 33342. The rodent cell lines hyperresistant to Hoechst 33342 and selected from AA8 were named AA8Hoe-R-1-AA8Hoe-R-10, and the cell lines selected from L cells were called LHoe-R-1-LHoe-R-10. The most resistant of them, AA8Hoe-R-6 and AA8Hoe-R-7, were able to grow in the presence of 80 μm/ml of Hoechst 33342 in the cell culture medium. All mutants were analyzed with the flow cytometric technique and were divided into two different groups. We conclude that the drug resistance of the first group of cell lines was due to changes in transport proteins. The second group of the resistant cell lines was characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. As we believe, the enhanced level of 'DNA clearing' was caused by the amplification of some genes, because the gradual increase of Hoechst resistance in the same cell line resulted from the increase in the ability to remove the dye from DNA. These lines were shown to be also resistant to netropsin.\",\"correspondence_address1\":\"Levina, V.V.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation; email: levina@ombr.pnpi.spb.ru\",\"issn\":\"10236597\",\"coden\":\"MCBIE\",\"pubmed_id\":\"10512056\",\"language\":\"English\",\"abbrev_source_title\":\"Membr. Cell Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Levina1998883,\\r\\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},\\r\\ntitle={'DNA clearing' from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance},\\r\\njournal={Membrane and Cell Biology},\\r\\nyear={1998},\\r\\nvolume={12},\\r\\nnumber={6},\\r\\npages={883-893},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&partnerID=40&md5=693d0527f04b69d04f82f18f75215244},\\r\\naffiliation={St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation},\\r\\nabstract={Earlier, we have described the process of active dissociation or 'DNA clearing' from non-covalently bound agents in living mammalian cells. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds DNA in the minor groove tightly but non-covalently, was used for studying the interaction of non-covalently binding agents with DNA. Multiple drug resistance (MDR) in tumour cells is related to the expression of transport proteins that alter the cellular drug transport and distribution. Three different groups of genes (mdr, MRP, and LRP) and their products are implicated in MDR. To obtain new cell lines characterized by enhanced process of active dissociation of non-covalently bound agents from DNA or 'DNA clearing', we carried out step-by-step selection with increasing concentrations of Hoechst 33342. The rodent cell lines hyperresistant to Hoechst 33342 and selected from AA8 were named AA8Hoe-R-1-AA8Hoe-R-10, and the cell lines selected from L cells were called LHoe-R-1-LHoe-R-10. The most resistant of them, AA8Hoe-R-6 and AA8Hoe-R-7, were able to grow in the presence of 80 μm/ml of Hoechst 33342 in the cell culture medium. All mutants were analyzed with the flow cytometric technique and were divided into two different groups. We conclude that the drug resistance of the first group of cell lines was due to changes in transport proteins. The second group of the resistant cell lines was characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. As we believe, the enhanced level of 'DNA clearing' was caused by the amplification of some genes, because the gradual increase of Hoechst resistance in the same cell line resulted from the increase in the ability to remove the dye from DNA. These lines were shown to be also resistant to netropsin.},\\r\\ncorrespondence_address1={Levina, V.V.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation; email: levina@ombr.pnpi.spb.ru},\\r\\nissn={10236597},\\r\\ncoden={MCBIE},\\r\\npubmed_id={10512056},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Membr. Cell Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Levina, V.\",\"Varfolomeeva, E.\",\"Filatov, M.\"],\"key\":\"Levina1998883\",\"id\":\"Levina1998883\",\"bibbaseid\":\"levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&partnerID=40&md5=693d0527f04b69d04f82f18f75215244\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ivanovo\"],\"firstnames\":[\"M.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Smolin\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Influence of polyelectrolyte properties of DNA on cytosine protonation\",\"journal\":\"Molekulyarnaya Biologiya\",\"year\":\"1998\",\"volume\":\"32\",\"number\":\"4\",\"pages\":\"635-638\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&partnerID=40&md5=15f307298c0ecbf7063bce7528f1f30b\",\"issn\":\"00268984\",\"coden\":\"MOBIB\",\"pubmed_id\":\"9785567\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mol. Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ivanovo1998635,\\r\\nauthor={Ivanovo, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},\\r\\ntitle={Influence of polyelectrolyte properties of DNA on cytosine protonation},\\r\\njournal={Molekulyarnaya Biologiya},\\r\\nyear={1998},\\r\\nvolume={32},\\r\\nnumber={4},\\r\\npages={635-638},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&partnerID=40&md5=15f307298c0ecbf7063bce7528f1f30b},\\r\\nissn={00268984},\\r\\ncoden={MOBIB},\\r\\npubmed_id={9785567},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mol. Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ivanovo, M.\",\"Smolin, A.\",\"Shcherbakova, O.\",\"Filatov, M.\"],\"key\":\"Ivanovo1998635\",\"id\":\"Ivanovo1998635\",\"bibbaseid\":\"ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&partnerID=40&md5=15f307298c0ecbf7063bce7528f1f30b\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Safronova\"],\"firstnames\":[\"I.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260\",\"journal\":\"Microbiology\",\"year\":\"1998\",\"volume\":\"67\",\"number\":\"1\",\"pages\":\"23-27\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&partnerID=40&md5=492e8c62636367903a8e064e4bf17882\",\"affiliation\":\"Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation\",\"abstract\":\"Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cistrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in the growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6. © 1998 MAHK Hayka/lnterperiodica Publishing.\",\"author_keywords\":\"Alcaligenes xylosoxidans subsp. xylosoxidans; L-malate; Maleate cis-trans-isomerase; Maleic acid; Transformation\",\"correspondence_address1\":\"Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation\",\"issn\":\"00262617\",\"language\":\"English\",\"abbrev_source_title\":\"Microbiology\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova199823,\\r\\nauthor={Safronova, I.Yu. and Semenova, E.V.},\\r\\ntitle={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},\\r\\njournal={Microbiology},\\r\\nyear={1998},\\r\\nvolume={67},\\r\\nnumber={1},\\r\\npages={23-27},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&partnerID=40&md5=492e8c62636367903a8e064e4bf17882},\\r\\naffiliation={Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},\\r\\nabstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cistrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in the growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6. © 1998 MAHK Hayka/lnterperiodica Publishing.},\\r\\nauthor_keywords={Alcaligenes xylosoxidans subsp. xylosoxidans;  L-malate;  Maleate cis-trans-isomerase;  Maleic acid;  Transformation},\\r\\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},\\r\\nissn={00262617},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Microbiology},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Safronova, I.\",\"Semenova, E.\"],\"key\":\"Safronova199823\",\"id\":\"Safronova199823\",\"bibbaseid\":\"safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&partnerID=40&md5=492e8c62636367903a8e064e4bf17882\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Safronova\"],\"firstnames\":[\"I.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260\",\"journal\":\"Mikrobiologiya\",\"year\":\"1998\",\"volume\":\"67\",\"number\":\"1\",\"pages\":\"30-34\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&partnerID=40&md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c\",\"affiliation\":\"Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation\",\"abstract\":\"Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cisfrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6.\",\"author_keywords\":\"Alcaligenes xylosoxidans subsp; L-malate; Maleate cis-transisomerase; Maleic acid; Transformation; Xylosoxidans\",\"correspondence_address1\":\"Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation\",\"issn\":\"00263656\",\"coden\":\"MIKBA\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mikrobiologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Safronova199830,\\r\\nauthor={Safronova, I.Yu. and Semenova, E.V.},\\r\\ntitle={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},\\r\\njournal={Mikrobiologiya},\\r\\nyear={1998},\\r\\nvolume={67},\\r\\nnumber={1},\\r\\npages={30-34},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&partnerID=40&md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c},\\r\\naffiliation={Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},\\r\\nabstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cisfrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6.},\\r\\nauthor_keywords={Alcaligenes xylosoxidans subsp;  L-malate;  Maleate cis-transisomerase;  Maleic acid;  Transformation;  Xylosoxidans},\\r\\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},\\r\\nissn={00263656},\\r\\ncoden={MIKBA},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mikrobiologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Safronova, I.\",\"Semenova, E.\"],\"key\":\"Safronova199830\",\"id\":\"Safronova199830\",\"bibbaseid\":\"safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&partnerID=40&md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskin\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]}],\"title\":\"Methods for registration of spontaneous DNA instability in mammalian cells\",\"journal\":\"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis\",\"year\":\"1998\",\"volume\":\"403\",\"number\":\"1-2\",\"pages\":\"95-101\",\"doi\":\"10.1016/S0027-5107(98)00056-6\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&doi=10.1016%2fS0027-5107%2898%2900056-6&partnerID=40&md5=63c03315650103ce0876961fb7f3630c\",\"affiliation\":\"St. Petersburg Nucl. Phys. Institute, Gatchina, St. Petersburg 188350, Russian Federation\",\"abstract\":\"A phenomenon of spontaneous DNA instability displays itself as the low level of repair DNA synthesis that takes place during any cell cycle phases. However, there is a problem in detection of very low intensive repair DNA synthesis. This paper suggests two approaches to detect the spontaneous DNA instability. The first method involves a blockade of the DNA gaps sealing by a combination of inhibitors, hydroxyurea and arabinofuranosyl cytosine. An accumulation of single strand gaps leads to production of DNA double strand breaks and results to reproductive inactivation of cells. It was shown that registration of both these events by different methods (such as viscoelastometry of DNA, orthogonal pulse electrophoresis or comet assay for double strand breaks as well as effectiveness of colony growth for cell inactivation) may be used as suitable measure of the spontaneous DNA instability. The second approach bases on photolysis of bromodeoxyuridine incorporated into repair DNA patches during the spontaneous repair DNA synthesis. Long wave UV irradiation of cells containing bromodeoxyuridine labeled DNA stained with Hoechst 33342 causes their inactivation. Experimental results presented confirm that both methods actually detect the spontaneous DNA instability. It takes note of the spontaneous DNA instability varies for cells from different tissues and species and increases during aging.\",\"author_keywords\":\"DNA synthesis inhibition; Repair DNA synthesis; Spontaneous DNA instability\",\"correspondence_address1\":\"Filatov, M.V.; St. Petersburg Nuclear Physics Inst., Gatchina, St. Petersburg 188350, Russian Federation; email: filatov@omrb.pnpi.spb.ru\",\"issn\":\"00275107\",\"coden\":\"MRFME\",\"pubmed_id\":\"9726010\",\"language\":\"English\",\"abbrev_source_title\":\"Mutat. Res. Fundam. Mol. Mech. Mutagen.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov199895,\\r\\nauthor={Filatov, M.V. and Pantina, R.A. and Noskin, L.A.},\\r\\ntitle={Methods for registration of spontaneous DNA instability in mammalian cells},\\r\\njournal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},\\r\\nyear={1998},\\r\\nvolume={403},\\r\\nnumber={1-2},\\r\\npages={95-101},\\r\\ndoi={10.1016/S0027-5107(98)00056-6},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&doi=10.1016%2fS0027-5107%2898%2900056-6&partnerID=40&md5=63c03315650103ce0876961fb7f3630c},\\r\\naffiliation={St. Petersburg Nucl. Phys. Institute, Gatchina, St. Petersburg 188350, Russian Federation},\\r\\nabstract={A phenomenon of spontaneous DNA instability displays itself as the low level of repair DNA synthesis that takes place during any cell cycle phases. However, there is a problem in detection of very low intensive repair DNA synthesis. This paper suggests two approaches to detect the spontaneous DNA instability. The first method involves a blockade of the DNA gaps sealing by a combination of inhibitors, hydroxyurea and arabinofuranosyl cytosine. An accumulation of single strand gaps leads to production of DNA double strand breaks and results to reproductive inactivation of cells. It was shown that registration of both these events by different methods (such as viscoelastometry of DNA, orthogonal pulse electrophoresis or comet assay for double strand breaks as well as effectiveness of colony growth for cell inactivation) may be used as suitable measure of the spontaneous DNA instability. The second approach bases on photolysis of bromodeoxyuridine incorporated into repair DNA patches during the spontaneous repair DNA synthesis. Long wave UV irradiation of cells containing bromodeoxyuridine labeled DNA stained with Hoechst 33342 causes their inactivation. Experimental results presented confirm that both methods actually detect the spontaneous DNA instability. It takes note of the spontaneous DNA instability varies for cells from different tissues and species and increases during aging.},\\r\\nauthor_keywords={DNA synthesis inhibition;  Repair DNA synthesis;  Spontaneous DNA instability},\\r\\ncorrespondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Inst., Gatchina, St. Petersburg 188350, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\\r\\nissn={00275107},\\r\\ncoden={MRFME},\\r\\npubmed_id={9726010},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Pantina, R.\",\"Noskin, L.\"],\"key\":\"Filatov199895\",\"id\":\"Filatov199895\",\"bibbaseid\":\"filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&doi=10.1016%2fS0027-5107%2898%2900056-6&partnerID=40&md5=63c03315650103ce0876961fb7f3630c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Ivanova\"],\"firstnames\":[\"M.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Smolin\"],\"firstnames\":[\"A.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Influence of polyelectrolyte properties of DNA on cytosine protonation\",\"journal\":\"Molecular Biology\",\"year\":\"1998\",\"volume\":\"32\",\"number\":\"4\",\"pages\":\"523-526\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&partnerID=40&md5=913fa635ede6f8418b0b492cae1970a4\",\"affiliation\":\"St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation\",\"abstract\":\"The polyelectrolyte properties of DNA affect cytosine protonation. The existence of a local region of lower pH in close proximity to the DNA chain is supposed to explain this phenomenon. Such a region was revealed by absorption spectroscopy with the use of Methyl Red (C15H15N3O2). Differences in the protonation of cytosines in different polyelectrolyte environments were detected spectrophotometrically under identical ionic and other conditions. The polyelectrolyte properties of DNA were proved to determine cytosine protonation at physiological pH. The differences in the protonation of cytosines were also observed in the studies of double-stranded high-and low-molecular DNA of the same nucleotide composition at low ionic strengths. The protonation of cytosine is shifted to the lower pH region for the high-molecular DNA as compared with fragments of lower molecular weight.\",\"author_keywords\":\"Absorption spectroscopy; Cytosine protonation; DNA; Local pH; Oligonucleotides; Polyelectrolyte properties\",\"correspondence_address1\":\"Ivanova, M.A.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: maria@omrb.pnpi.spb.ru\",\"issn\":\"00268933\",\"language\":\"English\",\"abbrev_source_title\":\"Mol. Biol.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Ivanova1998523,\\r\\nauthor={Ivanova, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},\\r\\ntitle={Influence of polyelectrolyte properties of DNA on cytosine protonation},\\r\\njournal={Molecular Biology},\\r\\nyear={1998},\\r\\nvolume={32},\\r\\nnumber={4},\\r\\npages={523-526},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&partnerID=40&md5=913fa635ede6f8418b0b492cae1970a4},\\r\\naffiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\\r\\nabstract={The polyelectrolyte properties of DNA affect cytosine protonation. The existence of a local region of lower pH in close proximity to the DNA chain is supposed to explain this phenomenon. Such a region was revealed by absorption spectroscopy with the use of Methyl Red (C15H15N3O2). Differences in the protonation of cytosines in different polyelectrolyte environments were detected spectrophotometrically under identical ionic and other conditions. The polyelectrolyte properties of DNA were proved to determine cytosine protonation at physiological pH. The differences in the protonation of cytosines were also observed in the studies of double-stranded high-and low-molecular DNA of the same nucleotide composition at low ionic strengths. The protonation of cytosine is shifted to the lower pH region for the high-molecular DNA as compared with fragments of lower molecular weight.},\\r\\nauthor_keywords={Absorption spectroscopy;  Cytosine protonation;  DNA;  Local pH;  Oligonucleotides;  Polyelectrolyte properties},\\r\\ncorrespondence_address1={Ivanova, M.A.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: maria@omrb.pnpi.spb.ru},\\r\\nissn={00268933},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mol. Biol.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Ivanova, M.\",\"Smolin, A.\",\"Shcherbakova, O.\",\"Filatov, M.\"],\"key\":\"Ivanova1998523\",\"id\":\"Ivanova1998523\",\"bibbaseid\":\"ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&partnerID=40&md5=913fa635ede6f8418b0b492cae1970a4\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Levina\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Yu.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tretyakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Klopov\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines\",\"journal\":\"Tsitologiya\",\"year\":\"1998\",\"volume\":\"40\",\"number\":\"10\",\"pages\":\"898-899\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&partnerID=40&md5=b71f1bc0675e0c35e7470ea90070a032\",\"affiliation\":\"Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"abstract\":\"The process of active dissociation or «DNA clearing» of not covalently binding agents from DNA in living HeLa cells was shown by the flow cytometry technique. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds tightly but not covalently to DNA in a minor groove, was used as a basic model to study the interaction of not covalently binding agents with DNA. In this paper, we continue to analyse the «DNA clearing» process in the living fibroblasts of different rodent species (mouse, rat, Chinese hamster). The obtained data suggest that the processes of active dissociation or «DNA clearing» of Hoechst 33342 have some common features in all investigated mammalian cells. Nevertheless, some differences in the process were found in these lines. The role of nucleotide excision repair genes in the process of DNA clearing was not established.\",\"correspondence_address1\":\"Levina, V.V.; Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"9864821\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiya\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Levina1998898,\\r\\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Drobchenko, E.A. and Tretyakov, A.N. and Klopov, N.V. and Filatov, M.V.},\\r\\ntitle={Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines},\\r\\njournal={Tsitologiya},\\r\\nyear={1998},\\r\\nvolume={40},\\r\\nnumber={10},\\r\\npages={898-899},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&partnerID=40&md5=b71f1bc0675e0c35e7470ea90070a032},\\r\\naffiliation={Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\nabstract={The process of active dissociation or «DNA clearing» of not covalently binding agents from DNA in living HeLa cells was shown by the flow cytometry technique. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds tightly but not covalently to DNA in a minor groove, was used as a basic model to study the interaction of not covalently binding agents with DNA. In this paper, we continue to analyse the «DNA clearing» process in the living fibroblasts of different rodent species (mouse, rat, Chinese hamster). The obtained data suggest that the processes of active dissociation or «DNA clearing» of Hoechst 33342 have some common features in all investigated mammalian cells. Nevertheless, some differences in the process were found in these lines. The role of nucleotide excision repair genes in the process of DNA clearing was not established.},\\r\\ncorrespondence_address1={Levina, V.V.; Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={9864821},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiya},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Levina, V.\",\"Varfolomeeva, E.\",\"Drobchenko, E.\",\"Tretyakov, A.\",\"Klopov, N.\",\"Filatov, M.\"],\"key\":\"Levina1998898\",\"id\":\"Levina1998898\",\"bibbaseid\":\"levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&partnerID=40&md5=b71f1bc0675e0c35e7470ea90070a032\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Tretyakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kaboev\"],\"firstnames\":[\"O.K.\"],\"suffixes\":[]}],\"title\":\"Fast DNA Amplification in Tiny Ultrathin Microplates\",\"journal\":\"Bioorganicheskaya Khimiya\",\"year\":\"1997\",\"volume\":\"23\",\"number\":\"6\",\"pages\":\"527-528\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&partnerID=40&md5=f26acee6ab5c8d940ffeac0eb2340e22\",\"affiliation\":\"Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation\",\"abstract\":\"A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 μm) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 μl samples and a high velocity of heating and cooling of the thermoblock (up to 7°C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.\",\"author_keywords\":\"Fast DNA amplification; Microplate; Polymerase chain reaction\",\"correspondence_address1\":\"Tretyakov, A.N.; Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation\",\"publisher\":\"Maik Nauka-Interperiodica Publishing\",\"issn\":\"01323423\",\"pubmed_id\":\"9265475\",\"language\":\"Russian\",\"abbrev_source_title\":\"Bioorganicheskaya Khim.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Tretyakov1997527,\\r\\nauthor={Tretyakov, A.N. and Pantina, R.A. and Kaboev, O.K.},\\r\\ntitle={Fast DNA Amplification in Tiny Ultrathin Microplates},\\r\\njournal={Bioorganicheskaya Khimiya},\\r\\nyear={1997},\\r\\nvolume={23},\\r\\nnumber={6},\\r\\npages={527-528},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&partnerID=40&md5=f26acee6ab5c8d940ffeac0eb2340e22},\\r\\naffiliation={Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},\\r\\nabstract={A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 μm) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 μl samples and a high velocity of heating and cooling of the thermoblock (up to 7°C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.},\\r\\nauthor_keywords={Fast DNA amplification;  Microplate;  Polymerase chain reaction},\\r\\ncorrespondence_address1={Tretyakov, A.N.; Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},\\r\\npublisher={Maik Nauka-Interperiodica Publishing},\\r\\nissn={01323423},\\r\\npubmed_id={9265475},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Bioorganicheskaya Khim.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Tretyakov, A.\",\"Pantina, R.\",\"Kaboev, O.\"],\"key\":\"Tretyakov1997527\",\"id\":\"Tretyakov1997527\",\"bibbaseid\":\"tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&partnerID=40&md5=f26acee6ab5c8d940ffeac0eb2340e22\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ivanov\"],\"firstnames\":[\"E.I.\"],\"suffixes\":[]}],\"title\":\"Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils\",\"journal\":\"Biochemical and Molecular Medicine\",\"year\":\"1995\",\"volume\":\"55\",\"number\":\"2\",\"pages\":\"116-121\",\"doi\":\"10.1006/bmme.1995.1041\",\"note\":\"cited By 27\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&doi=10.1006%2fbmme.1995.1041&partnerID=40&md5=adda09c8d4cb05b774fbe164b90edd86\",\"affiliation\":\"Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation\",\"abstract\":\"This report describes a method for measuring the respiratory burst in neutrophils, based on intracellular oxidation of the reduced ethidium bromide derivative, hydroethidine. Fluorescence of the resultant product quantitatively determined by flow cytofluorometry serves as a measure of the neutrophil ability to generate superoxide radicals. We found that during inflammation some polymorphonuclear leukocytes showed a considerably lower respiratory burst response to phorbol myristate acetate treatment. It was demonstrated that variations in this parameter could be an indicator of the course of inflammation. © 1995 by Academic Press, Inc.\",\"correspondence_address1\":\"Filatov, M.V.; Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation\",\"issn\":\"10773150\",\"language\":\"English\",\"abbrev_source_title\":\"BIOCHEM. MOL. MED.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1995116,\\r\\nauthor={Filatov, M.V. and Varfolomeeva, E.Y. and Ivanov, E.I.},\\r\\ntitle={Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils},\\r\\njournal={Biochemical and Molecular Medicine},\\r\\nyear={1995},\\r\\nvolume={55},\\r\\nnumber={2},\\r\\npages={116-121},\\r\\ndoi={10.1006/bmme.1995.1041},\\r\\nnote={cited By 27},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&doi=10.1006%2fbmme.1995.1041&partnerID=40&md5=adda09c8d4cb05b774fbe164b90edd86},\\r\\naffiliation={Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},\\r\\nabstract={This report describes a method for measuring the respiratory burst in neutrophils, based on intracellular oxidation of the reduced ethidium bromide derivative, hydroethidine. Fluorescence of the resultant product quantitatively determined by flow cytofluorometry serves as a measure of the neutrophil ability to generate superoxide radicals. We found that during inflammation some polymorphonuclear leukocytes showed a considerably lower respiratory burst response to phorbol myristate acetate treatment. It was demonstrated that variations in this parameter could be an indicator of the course of inflammation. © 1995 by Academic Press, Inc.},\\r\\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},\\r\\nissn={10773150},\\r\\nlanguage={English},\\r\\nabbrev_source_title={BIOCHEM. MOL. MED.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Varfolomeeva, E.\",\"Ivanov, E.\"],\"key\":\"Filatov1995116\",\"id\":\"Filatov1995116\",\"bibbaseid\":\"filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&doi=10.1006%2fbmme.1995.1041&partnerID=40&md5=adda09c8d4cb05b774fbe164b90edd86\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.Y.\"],\"suffixes\":[]}],\"title\":\"Active dissociation of Hoechst 33342 from DNA in living mammalian cells\",\"journal\":\"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis\",\"year\":\"1995\",\"volume\":\"327\",\"number\":\"1-2\",\"pages\":\"209-215\",\"doi\":\"10.1016/0027-5107(94)00189-C\",\"note\":\"cited By 20\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&doi=10.1016%2f0027-5107%2894%2900189-C&partnerID=40&md5=f0b6be765868fda9e94902fc88e4bc59\",\"affiliation\":\"St. Petersburg Nuclear Physics Institute, 188350 Gatchina St. Petersburg, Russian Federation\",\"abstract\":\"The fluorescent dye Hoechst 33342, which binds tightly to DNA in the minor groove, can be effectively extracted from the DNA in vivo due to an energy-dependent process. It is the dissociation of the dye from DNA rather than its removal from a cell that has a critical role in this process. The dissociation can be suppressed by the topoisomerase-2 inhibitors novobiocin, ellipticine and etoposide. Breaks in the DNA also inhibit the process. The dissociation of the dye requires that DNA remain intact along a region of at least several thousand base pairs. It is proposed that DNA in mammalian cells is a dynamic, conformationally nonstable system and that topologically closed loops consisting of several thousand base pairs constantly appear and disappear in chromatin. © 1995.\",\"author_keywords\":\"DNA conformation in vivo; Flow cytometry; Fluorescence dye\",\"key\":\"Filatov1995209\",\"id\":\"Filatov1995209\",\"bibbaseid\":\"anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&doi=10.1016%2f0027-5107%2894%2900189-C&partnerID=40&md5=f0b6be765868fda9e94902fc88e4bc59\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Tret\",\"id\":\"Tret\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Makhno\"],\"firstnames\":[\"V.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sedelnikova\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zhenodarova\"],\"firstnames\":[\"S.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kirillov\"],\"firstnames\":[\"S.V.\"],\"suffixes\":[]}],\"title\":\"Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction\",\"journal\":\"Molekulyarnaya Biologiya\",\"year\":\"1994\",\"volume\":\"28\",\"number\":\"1\",\"pages\":\"66-75\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&partnerID=40&md5=68e2dad0fa12c3f6e33f54dd5db8fb1f\",\"affiliation\":\"St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation\",\"abstract\":\"The method of anticodon loop replacement has been used to make derivatives of yeast tRNA((G(m)AAY(Phe)) with the substitution at the 37 position (tRNA(GAAA)(Phe)), and at both the anticodon (tRNA(GCAG)(Phe)) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(G(m)(AAY)(Phe), tRNA(GAAA)(Phe), tRNA(GCAG)(Phe), and tRNA(-Y)(Phe) with the P site of the 70S ribosome·poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalphy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U) the affinity of tRNA(-Y)(Phe) for the P site of the 70S ribosome is 5 times lower that the affinity of tRNA(G(m)AAY(Phe)) and tRNA(GCAG)(Phe). Thus, in the ribosome the mofified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon- anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome.\",\"correspondence_address1\":\"Katunin, V.I.; St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation\",\"issn\":\"00268984\",\"coden\":\"MOBIB\",\"pubmed_id\":\"8145756\",\"language\":\"Russian\",\"abbrev_source_title\":\"MOL. BIOL.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Katunin199466,\\r\\nauthor={Katunin, V.I. and Soboleva, N.G. and Makhno, V.I. and Sedelnikova, E.A. and Zhenodarova, S.M. and Kirillov, S.V.},\\r\\ntitle={Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction},\\r\\njournal={Molekulyarnaya Biologiya},\\r\\nyear={1994},\\r\\nvolume={28},\\r\\nnumber={1},\\r\\npages={66-75},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&partnerID=40&md5=68e2dad0fa12c3f6e33f54dd5db8fb1f},\\r\\naffiliation={St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},\\r\\nabstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNA((G(m)AAY(Phe)) with the substitution at the 37 position (tRNA(GAAA)(Phe)), and at both the anticodon (tRNA(GCAG)(Phe)) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(G(m)(AAY)(Phe), tRNA(GAAA)(Phe), tRNA(GCAG)(Phe), and tRNA(-Y)(Phe) with the P site of the 70S ribosome·poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalphy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U) the affinity of tRNA(-Y)(Phe) for the P site of the 70S ribosome is 5 times lower that the affinity of tRNA(G(m)AAY(Phe)) and tRNA(GCAG)(Phe). Thus, in the ribosome the mofified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon- anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome.},\\r\\ncorrespondence_address1={Katunin, V.I.; St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},\\r\\nissn={00268984},\\r\\ncoden={MOBIB},\\r\\npubmed_id={8145756},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={MOL. BIOL.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Katunin, V.\",\"Soboleva, N.\",\"Makhno, V.\",\"Sedelnikova, E.\",\"Zhenodarova, S.\",\"Kirillov, S.\"],\"key\":\"Katunin199466\",\"id\":\"Katunin199466\",\"bibbaseid\":\"katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&partnerID=40&md5=68e2dad0fa12c3f6e33f54dd5db8fb1f\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Katunin\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Soboleva\"],\"firstnames\":[\"N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Mahkno\"],\"firstnames\":[\"V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sedelnikova\"],\"firstnames\":[\"E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Zhenodarova\"],\"firstnames\":[\"S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kirillov\"],\"firstnames\":[\"S.\"],\"suffixes\":[]}],\"title\":\"Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes\",\"journal\":\"Biochimie\",\"year\":\"1994\",\"volume\":\"76\",\"number\":\"1\",\"pages\":\"51-57\",\"doi\":\"10.1016/0300-9084(94)90062-0\",\"note\":\"cited By 8\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&doi=10.1016%2f0300-9084%2894%2990062-0&partnerID=40&md5=28e38efb03ea6c49c3f74fdc2487ab60\",\"affiliation\":\"Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation; Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, 142292 Puschino, Moscow Region, Russian Federation\",\"abstract\":\"The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe with the substitution at position 37 (tRNAGAAAPhe) and at the anticodon(tRNAGCAGPhe). A quantitative study of the interaction of various types of deacylated yeast tRNAPhe (tRNA+YPhe, tRNAGAAAPhe, tRNA-yPhe) with the P site of the [70S ribosome*poly(U)]-complex was carried out at different Mg2+ concentrations and temperatures. The presence and nature of the nucleotide situated at the 3′-end of the anticodon are essential for such interaction in E coli ribosomes. Replacement of thee Y base with the unmodified adenosine decreases the interation enthalpy from 39 kcal/mol to 24 kcal/mol, whereas its removal reduces the interaction enthalpy to 16 kcal/mol. Replacement of the second anticodon nucleotide, adenosine, with cytosine further reduces the enthalphy to 6 kcal/mol, which is typical of tRNA-P site interaction in the absence of poly(U). In the absence of poly(U) the affinity of tRNA-YPhe for the P site of the 70S ribosome is five times lower than the affinity of tRNA+YPhe or tRNAGCAGPhe. Thus, in the ribosome the modified nucleotide stabilizes the codon-anticodon interaction through its stacking interaction with the codon-anticodon base stack. In addition, this decreases the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site. © 1994.\",\"author_keywords\":\"70S ribosome; codon-anticodon interaction; Escherichia coli; modified nucleotide; tRNAPhe\",\"correspondence_address1\":\"Katunin, V.; Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation\",\"issn\":\"03009084\",\"coden\":\"BICMB\",\"pubmed_id\":\"7518255\",\"language\":\"English\",\"abbrev_source_title\":\"Biochimie\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Katunin199451,\\r\\nauthor={Katunin, V. and Soboleva, N. and Mahkno, V. and Sedelnikova, E. and Zhenodarova, S. and Kirillov, S.},\\r\\ntitle={Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes},\\r\\njournal={Biochimie},\\r\\nyear={1994},\\r\\nvolume={76},\\r\\nnumber={1},\\r\\npages={51-57},\\r\\ndoi={10.1016/0300-9084(94)90062-0},\\r\\nnote={cited By 8},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&doi=10.1016%2f0300-9084%2894%2990062-0&partnerID=40&md5=28e38efb03ea6c49c3f74fdc2487ab60},\\r\\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation; Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, 142292 Puschino, Moscow Region, Russian Federation},\\r\\nabstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe with the substitution at position 37 (tRNAGAAAPhe) and at the anticodon(tRNAGCAGPhe). A quantitative study of the interaction of various types of deacylated yeast tRNAPhe (tRNA+YPhe, tRNAGAAAPhe, tRNA-yPhe) with the P site of the [70S ribosome*poly(U)]-complex was carried out at different Mg2+ concentrations and temperatures. The presence and nature of the nucleotide situated at the 3′-end of the anticodon are essential for such interaction in E coli ribosomes. Replacement of thee Y base with the unmodified adenosine decreases the interation enthalpy from 39 kcal/mol to 24 kcal/mol, whereas its removal reduces the interaction enthalpy to 16 kcal/mol. Replacement of the second anticodon nucleotide, adenosine, with cytosine further reduces the enthalphy to 6 kcal/mol, which is typical of tRNA-P site interaction in the absence of poly(U). In the absence of poly(U) the affinity of tRNA-YPhe for the P site of the 70S ribosome is five times lower than the affinity of tRNA+YPhe or tRNAGCAGPhe. Thus, in the ribosome the modified nucleotide stabilizes the codon-anticodon interaction through its stacking interaction with the codon-anticodon base stack. In addition, this decreases the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site. © 1994.},\\r\\nauthor_keywords={70S ribosome;  codon-anticodon interaction;  Escherichia coli;  modified nucleotide;  tRNAPhe},\\r\\ncorrespondence_address1={Katunin, V.; Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation},\\r\\nissn={03009084},\\r\\ncoden={BICMB},\\r\\npubmed_id={7518255},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Biochimie},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Katunin, V.\",\"Soboleva, N.\",\"Mahkno, V.\",\"Sedelnikova, E.\",\"Zhenodarova, S.\",\"Kirillov, S.\"],\"key\":\"Katunin199451\",\"id\":\"Katunin199451\",\"bibbaseid\":\"katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&doi=10.1016%2f0300-9084%2894%2990062-0&partnerID=40&md5=28e38efb03ea6c49c3f74fdc2487ab60\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Bulat\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kotlovanova\"],\"firstnames\":[\"L.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stepanov\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tret'iakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakova\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]}],\"title\":\"The production of clones of \\\"man x Chinese hamster\\\" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok \\\"chelovek x kitaǐskiǐ khomiachok\\\", soderzhashchikh raznye chasti genoma cheloveka.]\",\"journal\":\"Tsitologiya\",\"year\":\"1993\",\"volume\":\"35\",\"number\":\"6-7\",\"pages\":\"68-73\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&partnerID=40&md5=07712067d4e466fb44c89e58dfa85128\",\"abstract\":\"Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.\",\"correspondence_address1\":\"Filatov, M.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"8266566\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov199368,\\r\\nauthor={Filatov, M.V. and Bulat, S.A. and Drobchenko, E.A. and Kotlovanova, L.V. and Pantina, R.A. and Semenova, E.V. and Stepanov, S.I. and Tret'iakov, A.N. and Shcherbakova, O.G.},\\r\\ntitle={The production of clones of \\\"man x Chinese hamster\\\" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok \\\"chelovek x kitaǐskiǐ khomiachok\\\", soderzhashchikh raznye chasti genoma cheloveka.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1993},\\r\\nvolume={35},\\r\\nnumber={6-7},\\r\\npages={68-73},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&partnerID=40&md5=07712067d4e466fb44c89e58dfa85128},\\r\\nabstract={Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.},\\r\\ncorrespondence_address1={Filatov, M.V.},\\r\\nissn={00413771},\\r\\npubmed_id={8266566},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Bulat, S.\",\"Drobchenko, E.\",\"Kotlovanova, L.\",\"Pantina, R.\",\"Semenova, E.\",\"Stepanov, S.\",\"Tret'iakov, A.\",\"Shcherbakova, O.\"],\"key\":\"Filatov199368\",\"id\":\"Filatov199368\",\"bibbaseid\":\"filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&partnerID=40&md5=07712067d4e466fb44c89e58dfa85128\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bulat\"],\"firstnames\":[\"S.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]}],\"title\":\"The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.]\",\"journal\":\"Tsitologiya\",\"year\":\"1993\",\"volume\":\"35\",\"number\":\"6-7\",\"pages\":\"74-78\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&partnerID=40&md5=233809336d07245daae6c4fc6a081fa1\",\"abstract\":\"The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids \\\"human x Chinese hamster\\\" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.\",\"correspondence_address1\":\"Bulat, S.A.\",\"issn\":\"00413771\",\"pubmed_id\":\"8266567\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Bulat199374,\\r\\nauthor={Bulat, S.A. and Filatov, M.V. and Pantina, R.A.},\\r\\ntitle={The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1993},\\r\\nvolume={35},\\r\\nnumber={6-7},\\r\\npages={74-78},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&partnerID=40&md5=233809336d07245daae6c4fc6a081fa1},\\r\\nabstract={The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids \\\"human x Chinese hamster\\\" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.},\\r\\ncorrespondence_address1={Bulat, S.A.},\\r\\nissn={00413771},\\r\\npubmed_id={8266567},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Bulat, S.\",\"Filatov, M.\",\"Pantina, R.\"],\"key\":\"Bulat199374\",\"id\":\"Bulat199374\",\"bibbaseid\":\"bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&partnerID=40&md5=233809336d07245daae6c4fc6a081fa1\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Luniak\"],\"firstnames\":[\"V.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ibatullin\"],\"firstnames\":[\"F.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Timchenko\"],\"firstnames\":[\"N.A.\"],\"suffixes\":[]}],\"title\":\"The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel'nosti DARC146.]\",\"journal\":\"Tsitologiya\",\"year\":\"1993\",\"volume\":\"35\",\"number\":\"5\",\"pages\":\"84-90\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&partnerID=40&md5=a0bd6d6a1eee3ff3c287dde1598ec2f6\",\"abstract\":\"The binding of nuclear proteins to a DARC146 DNA fragment is described. The DARC146 was isolated from a complex form of DNA polymerase alpha. BrdUrd substitution experiments indicate that DARC146 can support an autonomous replication in mammalian cells. Three AAA blocks separated by 10 nucleotides were identified in the DARC146 sequence. Measuring electrophoretical mobility under appropriate conditions showed that these AAA blocks form a bent DNA. We have used a synthetic oligonucleotide covering the bent DNA to study the interaction of nuclear proteins with this DNA region. Four DNA-protein complexes with the bent DNA region were registered. One of them is formed by binding nuclear factor p65 to TCTATTA nucleotides. The molecular weight and binding site of p65 are very similar to those of c-myc protein. However, antibodies against c-myc protein exert no effect on the formation of the p65-DNA complex. We suggest that p65 is an unknown nuclear factor.\",\"correspondence_address1\":\"Luniak, V.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"8379011\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Luniak199384,\\r\\nauthor={Luniak, V.V. and Filatov, M.V. and Ibatullin, F.M. and Timchenko, N.A.},\\r\\ntitle={The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel'nosti DARC146.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1993},\\r\\nvolume={35},\\r\\nnumber={5},\\r\\npages={84-90},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&partnerID=40&md5=a0bd6d6a1eee3ff3c287dde1598ec2f6},\\r\\nabstract={The binding of nuclear proteins to a DARC146 DNA fragment is described. The DARC146 was isolated from a complex form of DNA polymerase alpha. BrdUrd substitution experiments indicate that DARC146 can support an autonomous replication in mammalian cells. Three AAA blocks separated by 10 nucleotides were identified in the DARC146 sequence. Measuring electrophoretical mobility under appropriate conditions showed that these AAA blocks form a bent DNA. We have used a synthetic oligonucleotide covering the bent DNA to study the interaction of nuclear proteins with this DNA region. Four DNA-protein complexes with the bent DNA region were registered. One of them is formed by binding nuclear factor p65 to TCTATTA nucleotides. The molecular weight and binding site of p65 are very similar to those of c-myc protein. However, antibodies against c-myc protein exert no effect on the formation of the p65-DNA complex. We suggest that p65 is an unknown nuclear factor.},\\r\\ncorrespondence_address1={Luniak, V.V.},\\r\\nissn={00413771},\\r\\npubmed_id={8379011},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Luniak, V.\",\"Filatov, M.\",\"Ibatullin, F.\",\"Timchenko, N.\"],\"key\":\"Luniak199384\",\"id\":\"Luniak199384\",\"bibbaseid\":\"luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&partnerID=40&md5=a0bd6d6a1eee3ff3c287dde1598ec2f6\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Arkad\",\"id\":\"Arkad\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bazhanov\"],\"firstnames\":[\"N.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ginzburg\"],\"firstnames\":[\"L.I.\"],\"suffixes\":[]}],\"title\":\"The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol'nykh revmatoidnym artritom.]\",\"journal\":\"Terapevticheskii Arkhiv\",\"year\":\"1992\",\"volume\":\"64\",\"number\":\"2\",\"pages\":\"93-96\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&partnerID=40&md5=cadd0e27379a5b1b7917b210b3fc1a1c\",\"abstract\":\"As a result of the work carried out, one could decide some problems: to develop standardized points of measuring temperatures of the deep structures of the joints using radiothermography, to establish for the first time the normal radiothermographic values of the temperatures for different joint groups, to recommend radiothermography as an adjuvant method for revealing early signs of the activity of rheumatoid arthritis (RA) associated with OA. Radiothermography is a convenient, cheap, available and an informative enough method, fit for the use in clinical practice to improve the diagnosis and treatment of RA.\",\"correspondence_address1\":\"Bazhanov, N.N.\",\"issn\":\"00403660\",\"pubmed_id\":\"1509395\",\"language\":\"Russian\",\"abbrev_source_title\":\"Ter Arkh\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Bazhanov199293,\\r\\nauthor={Bazhanov, N.N. and Semenova, E.V. and Ginzburg, L.I.},\\r\\ntitle={The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol'nykh revmatoidnym artritom.]},\\r\\njournal={Terapevticheskii Arkhiv},\\r\\nyear={1992},\\r\\nvolume={64},\\r\\nnumber={2},\\r\\npages={93-96},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&partnerID=40&md5=cadd0e27379a5b1b7917b210b3fc1a1c},\\r\\nabstract={As a result of the work carried out, one could decide some problems: to develop standardized points of measuring temperatures of the deep structures of the joints using radiothermography, to establish for the first time the normal radiothermographic values of the temperatures for different joint groups, to recommend radiothermography as an adjuvant method for revealing early signs of the activity of rheumatoid arthritis (RA) associated with OA. Radiothermography is a convenient, cheap, available and an informative enough method, fit for the use in clinical practice to improve the diagnosis and treatment of RA.},\\r\\ncorrespondence_address1={Bazhanov, N.N.},\\r\\nissn={00403660},\\r\\npubmed_id={1509395},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Ter Arkh},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Bazhanov, N.\",\"Semenova, E.\",\"Ginzburg, L.\"],\"key\":\"Bazhanov199293\",\"id\":\"Bazhanov199293\",\"bibbaseid\":\"bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&partnerID=40&md5=cadd0e27379a5b1b7917b210b3fc1a1c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"key\":\"Arkad-1\",\"id\":\"Arkad-1\",\"bibbaseid\":\"anonymous-\",\"role\":\"\",\"urls\":{},\"metadata\":{\"authorlinks\":{\"moore, a\":\"https://www.cl.cam.ac.uk/~awm22/papers.html\",\"powner, m\":\"https://bibbase.org/show?bib=benjaminthoma.github.io%2Fpubs.bib\",\"bergmann, r\":\"https://bibbase.org/show?bib=www.wi2.uni-trier.de/publications/WI2Publikationen.bib&group0=year&css=www.wi2.uni-trier.de/publications/WI2.css&filter=authors:Bergmann\",\"van harmelen, f\":\"https://www.cs.vu.nl/\",\"figueroa, n\":\"https://nbfigueroa.github.io/\",\"sammak, s\":\"https://shervinsammak.github.io/papers/\",\"patel, n\":\"https://niravatgit.github.io/INSPIRELab/publications.html#publications\",\"kouamé, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2023%2F07%2Fpubli_dk_Juillet2023.bib&commas=true&msg=embed&noBootstrap=1\",\"grossi, g\":\"https://bibbase.org/show?bib=https://homes.di.unimi.it/grossi/Grossi_Giuliano.bib\",\"alexiev, v\":\"https://rawgit2.com/VladimirAlexiev/my/master/index.html\",\"bethard, s\":\"https://bethard.github.io/publications.html\",\"homburg, t\":\"https://situx.github.io/publications/\",\"banzhaf, w\":\"https://cse.msu.edu/~banzhafw/Publications_new.html\",\"gatica, j\":\"https://bibbase.org/show?bib=www2.uca.es%2Fdept%2Fcmat_qinor%2Fnanomat%2Fpeople%2FGatica.bib&groupby=year\",\"edwards, c\":\"https://people.ucsc.edu/\",\"miyagusuku, r\":\"https://www.real.utsunomiya-u.ac.jp/invmap/\",\"stephens-davidowitz, n\":\"http://www.noahsd.com/\",\"kouam�, d\":\"https://bibbase.org/show?bib=https%3A%2F%2Fwww.irit.fr%2F%7EDenis.Kouame%2Fwp-content%2Fuploads%2Fsites%2F16%2F2020%2F08%2Fpubli_dk_pc.bib&msg=embed\",\"lee, u\":\"https://bibbase.org/show?bib=https://dblp.org/pid/172/3208.bib\",\"sohrabi, s\":\"https://bibbase.org/show?bib=www.cs.toronto.edu/~shirin/list.bib\",\"mcintire, e\":\"https://predictiveecology.org/publications\",\"mirrazavi salehian, s\":\"https://nbfigueroa.github.io/\",\"monteiro, s\":\"https://sildomar.github.io/publications/\",\"picco, m\":\"https://www.lpthe.jussieu.fr/~picco/pub.html\",\"maranas, c\":\"https://bibbase.org/show?bib=www.maranasgroup.com/pub/maranasgroup.bib\",\"romoli, j\":\"https://www.jacoporomoli.com/publications/\",\"maizel, a\":\"https://www.cos.uni-heidelberg.de/\",\"iovino, l\":\"https://www.ludovicoiovino.it/\",\"keller, a\":\"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt&folding=1\",\"riviere, b\":\"http://compm.rice.edu/publications/\",\"beyer, a\":\"https://www.klassphil.hu-berlin.de/de/personen/andrea-beyer\",\"bluestein, d\":\"https://www.bme.stonybrook.edu/labs/dbluestein/tavr.html\",\"sabattini, l\":\"https://34431841-atari-embeds.googleusercontent.com/embeds/16cb204cf3a9d4d223a0a3fd8b0eec5d/inner-frame-minified.html\"}},\"downloads\":9,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeev\"],\"firstnames\":[\"D.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kotlovanova\"],\"firstnames\":[\"L.V.\"],\"suffixes\":[]}],\"title\":\"New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.]\",\"journal\":\"Tsitologiya\",\"year\":\"1991\",\"volume\":\"33\",\"number\":\"4\",\"pages\":\"115-120\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&partnerID=40&md5=665c584f8ca0c771026cfbf0048ba0be\",\"abstract\":\"A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.\",\"correspondence_address1\":\"Filatov, M.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"1803700\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1991115,\\r\\nauthor={Filatov, M.V. and Varfolomeev, D.B. and Kotlovanova, L.V.},\\r\\ntitle={New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1991},\\r\\nvolume={33},\\r\\nnumber={4},\\r\\npages={115-120},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&partnerID=40&md5=665c584f8ca0c771026cfbf0048ba0be},\\r\\nabstract={A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.},\\r\\ncorrespondence_address1={Filatov, M.V.},\\r\\nissn={00413771},\\r\\npubmed_id={1803700},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Varfolomeev, D.\",\"Kotlovanova, L.\"],\"key\":\"Filatov1991115\",\"id\":\"Filatov1991115\",\"bibbaseid\":\"filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&partnerID=40&md5=665c584f8ca0c771026cfbf0048ba0be\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Meerson\"],\"firstnames\":[\"F.Z.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malyshev\"],\"firstnames\":[\"I.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\"],\"firstnames\":[\"E.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeev\"],\"firstnames\":[\"O.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskin\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]}],\"title\":\"Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost' iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.]\",\"journal\":\"Byulleten Eksperimentalnoi Biologii i Meditsiny\",\"year\":\"1991\",\"volume\":\"111\",\"number\":\"5\",\"pages\":\"460-462\",\"note\":\"cited By 4\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&partnerID=40&md5=6def35ea402425f2f367f48691d8c2af\",\"abstract\":\"The effect of adaptation to stress exposure on the resistance of nuclear DNA to damaging effect of one-chain exogenous DNA was investigated by flow cytometric fluorescence method. It was shown that after the administration of one-chain DNA in concentration of 50 micrograms/ml into the nuclei suspension almost half of nuclear DNA was damaged in control. In adaptation this phenomenon was 5.5 times less pronounced. When the one-chain DNA concentration was increased, this protective effect of adaptation remained. The possible mechanism of the DNA-protective effect of adaptation is under consideration.\",\"correspondence_address1\":\"Meerson, F.Z.\",\"issn\":\"03659615\",\"pubmed_id\":\"1878555\",\"language\":\"Russian\",\"abbrev_source_title\":\"Biull Eksp Biol Med\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Meerson1991460,\\r\\nauthor={Meerson, F.Z. and Malyshev, I.I. and Varfolomeeva, E.I. and Varfolomeev, O.B. and Noskin, A.N.},\\r\\ntitle={Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost' iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.]},\\r\\njournal={Byulleten Eksperimentalnoi Biologii i Meditsiny},\\r\\nyear={1991},\\r\\nvolume={111},\\r\\nnumber={5},\\r\\npages={460-462},\\r\\nnote={cited By 4},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&partnerID=40&md5=6def35ea402425f2f367f48691d8c2af},\\r\\nabstract={The effect of adaptation to stress exposure on the resistance of nuclear DNA to damaging effect of one-chain exogenous DNA was investigated by flow cytometric fluorescence method. It was shown that after the administration of one-chain DNA in concentration of 50 micrograms/ml into the nuclei suspension almost half of nuclear DNA was damaged in control. In adaptation this phenomenon was 5.5 times less pronounced. When the one-chain DNA concentration was increased, this protective effect of adaptation remained. The possible mechanism of the DNA-protective effect of adaptation is under consideration.},\\r\\ncorrespondence_address1={Meerson, F.Z.},\\r\\nissn={03659615},\\r\\npubmed_id={1878555},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Biull Eksp Biol Med},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Meerson, F.\",\"Malyshev, I.\",\"Varfolomeeva, E.\",\"Varfolomeev, O.\",\"Noskin, A.\"],\"key\":\"Meerson1991460\",\"id\":\"Meerson1991460\",\"bibbaseid\":\"meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&partnerID=40&md5=6def35ea402425f2f367f48691d8c2af\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Timchenko\"],\"firstnames\":[\"N.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shcherbakov\"],\"firstnames\":[\"O.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Krutyakov\"],\"firstnames\":[\"V.M.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex\",\"journal\":\"Molekulyarnaya Biologiya\",\"year\":\"1990\",\"volume\":\"24\",\"number\":\"3\",\"pages\":\"814-823\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&partnerID=40&md5=954a167f2eb8cd97e225fe80f81d78bc\",\"affiliation\":\"B.P. Konstantinov Nuclear Physics Institute, Academy of Sciences of the USSR, Gatchina, Leningrad Region 188350, Russia\",\"issn\":\"00268984\",\"coden\":\"MOBIB\",\"pubmed_id\":\"2169584\",\"language\":\"Russian\",\"abbrev_source_title\":\"MOL. BIOL.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Timchenko1990814,\\r\\nauthor={Timchenko, N.A. and Shcherbakov, O.G. and Krutyakov, V.M. and Filatov, M.V.},\\r\\ntitle={Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex},\\r\\njournal={Molekulyarnaya Biologiya},\\r\\nyear={1990},\\r\\nvolume={24},\\r\\nnumber={3},\\r\\npages={814-823},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&partnerID=40&md5=954a167f2eb8cd97e225fe80f81d78bc},\\r\\naffiliation={B.P. Konstantinov Nuclear Physics Institute, Academy of Sciences of the USSR, Gatchina, Leningrad Region 188350, Russia},\\r\\nissn={00268984},\\r\\ncoden={MOBIB},\\r\\npubmed_id={2169584},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={MOL. BIOL.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Timchenko, N.\",\"Shcherbakov, O.\",\"Krutyakov, V.\",\"Filatov, M.\"],\"key\":\"Timchenko1990814\",\"id\":\"Timchenko1990814\",\"bibbaseid\":\"timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&partnerID=40&md5=954a167f2eb8cd97e225fe80f81d78bc\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Streltsov\"],\"firstnames\":[\"P.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"A simple method for estimating the rate of repair synthesis in cultured mammalian cells\",\"journal\":\"Radiobiologiya\",\"year\":\"1990\",\"volume\":\"30\",\"number\":\"2\",\"pages\":\"279-282\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&partnerID=40&md5=b8496824b416bf690816ecd2ca1277da\",\"affiliation\":\"B.P. Konstantinov Leningrad Institute for Nuclear Physics, USSR Academy of Sciences, Gatchina, Russia\",\"issn\":\"00338192\",\"coden\":\"RADOA\",\"pubmed_id\":\"1693442\",\"language\":\"Russian\",\"abbrev_source_title\":\"RADIOBIOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Streltsov1990279,\\r\\nauthor={Streltsov, P.G. and Filatov, M.V.},\\r\\ntitle={A simple method for estimating the rate of repair synthesis in cultured mammalian cells},\\r\\njournal={Radiobiologiya},\\r\\nyear={1990},\\r\\nvolume={30},\\r\\nnumber={2},\\r\\npages={279-282},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&partnerID=40&md5=b8496824b416bf690816ecd2ca1277da},\\r\\naffiliation={B.P. Konstantinov Leningrad Institute for Nuclear Physics, USSR Academy of Sciences, Gatchina, Russia},\\r\\nissn={00338192},\\r\\ncoden={RADOA},\\r\\npubmed_id={1693442},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={RADIOBIOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Streltsov, P.\",\"Filatov, M.\"],\"key\":\"Streltsov1990279\",\"id\":\"Streltsov1990279\",\"bibbaseid\":\"streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&partnerID=40&md5=b8496824b416bf690816ecd2ca1277da\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Varfolomeeva\",\"Yu.\"],\"firstnames\":[\"E.\"],\"suffixes\":[]}],\"title\":\"A study of chromatin packing in the mammalian cell nuclei by flow cytometry\",\"journal\":\"Tsitologiya\",\"year\":\"1990\",\"volume\":\"32\",\"number\":\"4\",\"pages\":\"343-351\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&partnerID=40&md5=dfa0e0cab0df064bbdf027d880a7be2b\",\"affiliation\":\"Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Leningrad, Russia\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"language\":\"Russian\",\"abbrev_source_title\":\"TSITOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1990343,\\r\\nauthor={Filatov, M.V. and Varfolomeeva Yu., E.},\\r\\ntitle={A study of chromatin packing in the mammalian cell nuclei by flow cytometry},\\r\\njournal={Tsitologiya},\\r\\nyear={1990},\\r\\nvolume={32},\\r\\nnumber={4},\\r\\npages={343-351},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&partnerID=40&md5=dfa0e0cab0df064bbdf027d880a7be2b},\\r\\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Leningrad, Russia},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={TSITOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Varfolomeeva Yu., E.\"],\"key\":\"Filatov1990343\",\"id\":\"Filatov1990343\",\"bibbaseid\":\"filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&partnerID=40&md5=dfa0e0cab0df064bbdf027d880a7be2b\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Stepanov\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskin\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Drobchenko\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kotlovanova\"],\"firstnames\":[\"L.V.\"],\"suffixes\":[]}],\"title\":\"Cell-by-cell chromosomal flow cytometric analysis\",\"journal\":\"Tsitologiya\",\"year\":\"1989\",\"volume\":\"31\",\"number\":\"4\",\"pages\":\"410-418\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&partnerID=40&md5=06c63d8e6d241bf9aeaca3c56b5c6c29\",\"affiliation\":\"Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"2756567\",\"language\":\"Russian\",\"abbrev_source_title\":\"TSITOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Stepanov1989410,\\r\\nauthor={Stepanov, S.I. and Semenova, E.V. and Noskin, L.A. and Drobchenko, E.A. and Filatov, M.V. and Kotlovanova, L.V.},\\r\\ntitle={Cell-by-cell chromosomal flow cytometric analysis},\\r\\njournal={Tsitologiya},\\r\\nyear={1989},\\r\\nvolume={31},\\r\\nnumber={4},\\r\\npages={410-418},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&partnerID=40&md5=06c63d8e6d241bf9aeaca3c56b5c6c29},\\r\\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={2756567},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={TSITOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Stepanov, S.\",\"Semenova, E.\",\"Noskin, L.\",\"Drobchenko, E.\",\"Filatov, M.\",\"Kotlovanova, L.\"],\"key\":\"Stepanov1989410\",\"id\":\"Stepanov1989410\",\"bibbaseid\":\"stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&partnerID=40&md5=06c63d8e6d241bf9aeaca3c56b5c6c29\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kotlovanova\"],\"firstnames\":[\"L.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Stepanov\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Tret'iakov\"],\"firstnames\":[\"A.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Strel'tsov\"],\"firstnames\":[\"P.G.\"],\"suffixes\":[]}],\"title\":\"Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil'nost' khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch'iu protochnoǐ tsitometrii.]\",\"journal\":\"Tsitologiya\",\"year\":\"1988\",\"volume\":\"30\",\"number\":\"8\",\"pages\":\"999-1007\",\"note\":\"cited By 3\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&partnerID=40&md5=e1990ffa33950ed509f57f13cef44d1a\",\"abstract\":\"The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.\",\"correspondence_address1\":\"Filatov, M.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"3206547\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1988999,\\r\\nauthor={Filatov, M.V. and Kotlovanova, L.V. and Stepanov, S.I. and Tret'iakov, A.N. and Strel'tsov, P.G.},\\r\\ntitle={Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil'nost' khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch'iu protochnoǐ tsitometrii.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1988},\\r\\nvolume={30},\\r\\nnumber={8},\\r\\npages={999-1007},\\r\\nnote={cited By 3},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&partnerID=40&md5=e1990ffa33950ed509f57f13cef44d1a},\\r\\nabstract={The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.},\\r\\ncorrespondence_address1={Filatov, M.V.},\\r\\nissn={00413771},\\r\\npubmed_id={3206547},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Kotlovanova, L.\",\"Stepanov, S.\",\"Tret'iakov, A.\",\"Strel'tsov, P.\"],\"key\":\"Filatov1988999\",\"id\":\"Filatov1988999\",\"bibbaseid\":\"filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&partnerID=40&md5=e1990ffa33950ed509f57f13cef44d1a\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Pantina\"],\"firstnames\":[\"R.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Frolova\"],\"firstnames\":[\"L.I.\"],\"suffixes\":[]}],\"title\":\"For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.]\",\"journal\":\"Tsitologiya\",\"year\":\"1986\",\"volume\":\"28\",\"number\":\"12\",\"pages\":\"1364-1368\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&partnerID=40&md5=8038fcad140efcdbf1a4e95b7d4885ad\",\"abstract\":\"The Chinese hamster cells V-79 were treated with BUdR during one cell cycle; after that the cells were grown in the medium without BUdR and were irradiated by longwave-UV-light at different time. The cell survival after photolysis was compared with the percentage of metaphase plates with different number of chromosomes containing BUdR. It is concluded that for cell inactivation the presence of only one destroyed chromosome (or its part) is enough.\",\"correspondence_address1\":\"Filatov, M.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"3824524\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov19861364,\\r\\nauthor={Filatov, M.V. and Pantina, R.A. and Frolova, L.I.},\\r\\ntitle={For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1986},\\r\\nvolume={28},\\r\\nnumber={12},\\r\\npages={1364-1368},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&partnerID=40&md5=8038fcad140efcdbf1a4e95b7d4885ad},\\r\\nabstract={The Chinese hamster cells V-79 were treated with BUdR during one cell cycle; after that the cells were grown in the medium without BUdR and were irradiated by longwave-UV-light at different time. The cell survival after photolysis was compared with the percentage of metaphase plates with different number of chromosomes containing BUdR. It is concluded that for cell inactivation the presence of only one destroyed chromosome (or its part) is enough.},\\r\\ncorrespondence_address1={Filatov, M.V.},\\r\\nissn={00413771},\\r\\npubmed_id={3824524},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Pantina, R.\",\"Frolova, L.\"],\"key\":\"Filatov19861364\",\"id\":\"Filatov19861364\",\"bibbaseid\":\"filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&partnerID=40&md5=8038fcad140efcdbf1a4e95b7d4885ad\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Stepanova\"],\"firstnames\":[\"S.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Vershilova\"],\"firstnames\":[\"E.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Malinovskiǐ\"],\"firstnames\":[\"O.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]}],\"title\":\"Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.]\",\"journal\":\"Tsitologiia\",\"year\":\"1986\",\"volume\":\"28\",\"number\":\"5\",\"pages\":\"583-586\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&partnerID=40&md5=c5801fa59eddabd92ec6257a507b8d26\",\"abstract\":\"A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.\",\"correspondence_address1\":\"Stepanova, S.I.\",\"issn\":\"00413771\",\"pubmed_id\":\"3738992\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Stepanova1986583,\\r\\nauthor={Stepanova, S.I. and Vershilova, E.I. and Malinovskiǐ, O.V. and Filatov, M.V.},\\r\\ntitle={Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.]},\\r\\njournal={Tsitologiia},\\r\\nyear={1986},\\r\\nvolume={28},\\r\\nnumber={5},\\r\\npages={583-586},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&partnerID=40&md5=c5801fa59eddabd92ec6257a507b8d26},\\r\\nabstract={A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.},\\r\\ncorrespondence_address1={Stepanova, S.I.},\\r\\nissn={00413771},\\r\\npubmed_id={3738992},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Stepanova, S.\",\"Vershilova, E.\",\"Malinovskiǐ, O.\",\"Filatov, M.\"],\"key\":\"Stepanova1986583\",\"id\":\"Stepanova1986583\",\"bibbaseid\":\"stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&partnerID=40&md5=c5801fa59eddabd92ec6257a507b8d26\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Maximov\"],\"firstnames\":[\"V.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Volokitina\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"N.S.\"],\"suffixes\":[]}],\"title\":\"Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum\",\"journal\":\"Mikrobiologiya\",\"year\":\"1986\",\"volume\":\"55\",\"number\":\"4\",\"pages\":\"643-647\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&partnerID=40&md5=e0eb3de06bc13b567cab07d6a39ce3c5\",\"affiliation\":\"Moskovskij Gosudarstvennyj Universitet Biologicheskij Fakul'tet, Moskva, Russian Federation\",\"abstract\":\"The effect of some components of the medium on the growth of Mycobacterium cyaneum B-646 and on the biosynthesis of an exoglycan by the culture was studied. A medium in which the M. cyaneum M variant produced up to 2.2 g of the exopolysaccharide per litre, which was nearly 4 times more than in the original medium, was proposed. The new medium differed from the original one in an elevated content of carbon, iron and potassium (11.2, 5.0 and 1.4 times, respectively) and in a lower phosphorus content (6.7 times). The exopolysaccharide produced by the culture in this medium contained glucose, galactose, fucose and uronic acid. Therefore, its monomeric composition did not depend on the medium used for growing the culture which produced the exopolysaccharide.\",\"issn\":\"00263656\",\"coden\":\"MIKBA\",\"pubmed_id\":\"3773795\",\"language\":\"Russian\",\"abbrev_source_title\":\"MIKROBIOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Semenova1986643,\\r\\nauthor={Semenova, E.V. and Maximov, V.N. and Volokitina, M.V. and Egorov, N.S.},\\r\\ntitle={Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum},\\r\\njournal={Mikrobiologiya},\\r\\nyear={1986},\\r\\nvolume={55},\\r\\nnumber={4},\\r\\npages={643-647},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&partnerID=40&md5=e0eb3de06bc13b567cab07d6a39ce3c5},\\r\\naffiliation={Moskovskij Gosudarstvennyj Universitet Biologicheskij Fakul'tet, Moskva, Russian Federation},\\r\\nabstract={The effect of some components of the medium on the growth of Mycobacterium cyaneum B-646 and on the biosynthesis of an exoglycan by the culture was studied. A medium in which the M. cyaneum M variant produced up to 2.2 g of the exopolysaccharide per litre, which was nearly 4 times more than in the original medium, was proposed. The new medium differed from the original one in an elevated content of carbon, iron and potassium (11.2, 5.0 and 1.4 times, respectively) and in a lower phosphorus content (6.7 times). The exopolysaccharide produced by the culture in this medium contained glucose, galactose, fucose and uronic acid. Therefore, its monomeric composition did not depend on the medium used for growing the culture which produced the exopolysaccharide.},\\r\\nissn={00263656},\\r\\ncoden={MIKBA},\\r\\npubmed_id={3773795},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={MIKROBIOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Semenova, E.\",\"Maximov, V.\",\"Volokitina, M.\",\"Egorov, N.\"],\"key\":\"Semenova1986643\",\"id\":\"Semenova1986643\",\"bibbaseid\":\"semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&partnerID=40&md5=e0eb3de06bc13b567cab07d6a39ce3c5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Bresler\"],\"firstnames\":[\"S.E.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Davidenkova\"],\"firstnames\":[\"E.F.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shvarts\"],\"firstnames\":[\"E.I.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskin\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]}],\"title\":\"Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.]\",\"journal\":\"Radiobiologiya\",\"year\":\"1984\",\"volume\":\"24\",\"number\":\"3\",\"pages\":\"291-295\",\"note\":\"cited By 2\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&partnerID=40&md5=dbd6284fdad0eebea33f4423d212ddd6\",\"abstract\":\"A technique for registration of spontaneous DNA lesions in intact mammalian cells is proposed. Arabinosid cytosine and hydroxyurea were shown to inhibit the repair of gaps spontaneously formed and accumulated in DNA leading to the formation of double-strand DNA breaks and cell death. The rate of spontaneous lesions was different in cells from different animal species and different tissues. A considerable increase in the level of spontaneous lesions was noted with ageing. It is concluded that the level of spontaneous DNA lesions is one of the fundamental biologically important characteristics of a cell.\",\"correspondence_address1\":\"Bresler, S.E.\",\"issn\":\"00338192\",\"pubmed_id\":\"6739729\",\"language\":\"Russian\",\"abbrev_source_title\":\"Radiobiologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Bresler1984291,\\r\\nauthor={Bresler, S.E. and Davidenkova, E.F. and Filatov, M.V. and Shvarts, E.I. and Noskin, L.A.},\\r\\ntitle={Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.]},\\r\\njournal={Radiobiologiya},\\r\\nyear={1984},\\r\\nvolume={24},\\r\\nnumber={3},\\r\\npages={291-295},\\r\\nnote={cited By 2},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&partnerID=40&md5=dbd6284fdad0eebea33f4423d212ddd6},\\r\\nabstract={A technique for registration of spontaneous DNA lesions in intact mammalian cells is proposed. Arabinosid cytosine and hydroxyurea were shown to inhibit the repair of gaps spontaneously formed and accumulated in DNA leading to the formation of double-strand DNA breaks and cell death. The rate of spontaneous lesions was different in cells from different animal species and different tissues. A considerable increase in the level of spontaneous lesions was noted with ageing. It is concluded that the level of spontaneous DNA lesions is one of the fundamental biologically important characteristics of a cell.},\\r\\ncorrespondence_address1={Bresler, S.E.},\\r\\nissn={00338192},\\r\\npubmed_id={6739729},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Radiobiologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Bresler, S.\",\"Davidenkova, E.\",\"Filatov, M.\",\"Shvarts, E.\",\"Noskin, L.\"],\"key\":\"Bresler1984291\",\"id\":\"Bresler1984291\",\"bibbaseid\":\"bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&partnerID=40&md5=dbd6284fdad0eebea33f4423d212ddd6\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sheǐkina\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]}],\"title\":\"New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.]\",\"journal\":\"Tsitologiya\",\"year\":\"1984\",\"volume\":\"26\",\"number\":\"4\",\"pages\":\"450-457\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&partnerID=40&md5=993c307758a8f3695b9b6f979ad06bf8\",\"abstract\":\"A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.\",\"correspondence_address1\":\"Filatov, M.V.\",\"issn\":\"00413771\",\"pubmed_id\":\"6740764\",\"language\":\"Russian\",\"abbrev_source_title\":\"Tsitologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1984450,\\r\\nauthor={Filatov, M.V. and Sheǐkina, T.A.},\\r\\ntitle={New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.]},\\r\\njournal={Tsitologiya},\\r\\nyear={1984},\\r\\nvolume={26},\\r\\nnumber={4},\\r\\npages={450-457},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&partnerID=40&md5=993c307758a8f3695b9b6f979ad06bf8},\\r\\nabstract={A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.},\\r\\ncorrespondence_address1={Filatov, M.V.},\\r\\nissn={00413771},\\r\\npubmed_id={6740764},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Tsitologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Sheǐkina, T.\"],\"key\":\"Filatov1984450\",\"id\":\"Filatov1984450\",\"bibbaseid\":\"filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&partnerID=40&md5=993c307758a8f3695b9b6f979ad06bf8\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"N.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grechushkina\"],\"firstnames\":[\"N.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Botvinko\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sviridov\"],\"firstnames\":[\"A.F.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.]\",\"journal\":\"Mikrobiologiya\",\"year\":\"1984\",\"volume\":\"53\",\"number\":\"2\",\"pages\":\"199-202\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&partnerID=40&md5=8308484a481aa0f50924b9af171d5084\",\"abstract\":\"Exocellular polysaccharides were synthesized by ten strains of 7 saprotrophic mycobacterial species in parallel with their growth. The cultures grew and produced polysaccharides more rapidly in a medium with glucose than in a medium with n-hexadecane. The polymers are homogeneous acid heteroglycans of the regular structure. All of them contain glucose and some of them contain hexuronic acid. Galactose, fucose, mannose, ramnose and pyruvic acid are found in some of the polysaccharides. The exoglycans of two mycobacterial species contain mannolactylic acid. Beta-glycosidic linkages prevail in most of the polysaccharides.\",\"correspondence_address1\":\"Egorov, N.S.\",\"issn\":\"00263656\",\"pubmed_id\":\"6738386\",\"language\":\"Russian\",\"abbrev_source_title\":\"Mikrobiologiia\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Egorov1984199,\\r\\nauthor={Egorov, N.S. and Grechushkina, N.N. and Botvinko, I.V. and Sviridov, A.F. and Semenova, E.V.},\\r\\ntitle={Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.]},\\r\\njournal={Mikrobiologiya},\\r\\nyear={1984},\\r\\nvolume={53},\\r\\nnumber={2},\\r\\npages={199-202},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&partnerID=40&md5=8308484a481aa0f50924b9af171d5084},\\r\\nabstract={Exocellular polysaccharides were synthesized by ten strains of 7 saprotrophic mycobacterial species in parallel with their growth. The cultures grew and produced polysaccharides more rapidly in a medium with glucose than in a medium with n-hexadecane. The polymers are homogeneous acid heteroglycans of the regular structure. All of them contain glucose and some of them contain hexuronic acid. Galactose, fucose, mannose, ramnose and pyruvic acid are found in some of the polysaccharides. The exoglycans of two mycobacterial species contain mannolactylic acid. Beta-glycosidic linkages prevail in most of the polysaccharides.},\\r\\ncorrespondence_address1={Egorov, N.S.},\\r\\nissn={00263656},\\r\\npubmed_id={6738386},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Mikrobiologiia},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Egorov, N.\",\"Grechushkina, N.\",\"Botvinko, I.\",\"Sviridov, A.\",\"Semenova, E.\"],\"key\":\"Egorov1984199\",\"id\":\"Egorov1984199\",\"bibbaseid\":\"egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&partnerID=40&md5=8308484a481aa0f50924b9af171d5084\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sheikina\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]}],\"title\":\"Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation\",\"journal\":\"Tsitologiya\",\"year\":\"1984\",\"volume\":\"26\",\"number\":\"10\",\"pages\":\"1208-1212\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&partnerID=40&md5=bbdd1d28eb7da8606eae844f947424da\",\"affiliation\":\"Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation\",\"issn\":\"00413771\",\"coden\":\"TSITA\",\"pubmed_id\":\"6515721\",\"language\":\"Russian\",\"abbrev_source_title\":\"TSITOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov19841208,\\r\\nauthor={Filatov, M.V. and Sheikina, T.A.},\\r\\ntitle={Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation},\\r\\njournal={Tsitologiya},\\r\\nyear={1984},\\r\\nvolume={26},\\r\\nnumber={10},\\r\\npages={1208-1212},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&partnerID=40&md5=bbdd1d28eb7da8606eae844f947424da},\\r\\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},\\r\\nissn={00413771},\\r\\ncoden={TSITA},\\r\\npubmed_id={6515721},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={TSITOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Sheikina, T.\"],\"key\":\"Filatov19841208\",\"id\":\"Filatov19841208\",\"bibbaseid\":\"filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&partnerID=40&md5=bbdd1d28eb7da8606eae844f947424da\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Botvinko\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grechushkina\"],\"firstnames\":[\"N.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Rachinskaia\"],\"firstnames\":[\"L.G.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"N.S.\"],\"suffixes\":[]}],\"title\":\"Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul'tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.]\",\"journal\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"year\":\"1984\",\"number\":\"2\",\"pages\":\"89-92\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&partnerID=40&md5=58e4dbe1068fbdb5fb8908eaf0c1738c\",\"abstract\":\"The source of carbon and nitrogen as well as pH of medium influence the possibility of synthesis of the exocellular heteropolysaccharide of Mycobacterium cyaneum and the quantitative harvest of glucan but not its monomer composition. The conditions of mycobacterial growth and glucan synthesis usually do not coincide.\",\"correspondence_address1\":\"Botvinko, I.V.\",\"issn\":\"04704606\",\"pubmed_id\":\"6713026\",\"language\":\"Russian\",\"abbrev_source_title\":\"Nauchnye Doki Vyss Shkoly Biol Nauki\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Botvinko198489,\\r\\nauthor={Botvinko, I.V. and Grechushkina, N.N. and Rachinskaia, L.G. and Semenova, E.V. and Egorov, N.S.},\\r\\ntitle={Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul'tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.]},\\r\\njournal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},\\r\\nyear={1984},\\r\\nnumber={2},\\r\\npages={89-92},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&partnerID=40&md5=58e4dbe1068fbdb5fb8908eaf0c1738c},\\r\\nabstract={The source of carbon and nitrogen as well as pH of medium influence the possibility of synthesis of the exocellular heteropolysaccharide of Mycobacterium cyaneum and the quantitative harvest of glucan but not its monomer composition. The conditions of mycobacterial growth and glucan synthesis usually do not coincide.},\\r\\ncorrespondence_address1={Botvinko, I.V.},\\r\\nissn={04704606},\\r\\npubmed_id={6713026},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Botvinko, I.\",\"Grechushkina, N.\",\"Rachinskaia, L.\",\"Semenova, E.\",\"Egorov, N.\"],\"key\":\"Botvinko198489\",\"id\":\"Botvinko198489\",\"bibbaseid\":\"botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&partnerID=40&md5=58e4dbe1068fbdb5fb8908eaf0c1738c\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Oleinikova\"],\"firstnames\":[\"E.A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"N.S.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Anoshkina\"],\"firstnames\":[\"G.B.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Morozov\"],\"firstnames\":[\"A.T.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Botvinko\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]}],\"title\":\"Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection\",\"journal\":\"Bulletin of Experimental Biology and Medicine\",\"year\":\"1983\",\"volume\":\"95\",\"number\":\"3\",\"pages\":\"320-323\",\"doi\":\"10.1007/BF00830178\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&doi=10.1007%2fBF00830178&partnerID=40&md5=2d05f6b733ea895fd47478957ed6c488\",\"author_keywords\":\"cell enzymes; cell reaction; macrophages; mycobacterial polysaccharide; phagocytosis\",\"publisher\":\"Kluwer Academic Publishers-Plenum Publishers\",\"issn\":\"00074888\",\"coden\":\"BEXBA\",\"language\":\"English\",\"abbrev_source_title\":\"Bull Exp Biol Med\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Oleinikova1983320,\\r\\nauthor={Oleinikova, E.A. and Egorov, N.S. and Anoshkina, G.B. and Morozov, A.T. and Botvinko, I.V. and Semenova, E.V.},\\r\\ntitle={Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection},\\r\\njournal={Bulletin of Experimental Biology and Medicine},\\r\\nyear={1983},\\r\\nvolume={95},\\r\\nnumber={3},\\r\\npages={320-323},\\r\\ndoi={10.1007/BF00830178},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&doi=10.1007%2fBF00830178&partnerID=40&md5=2d05f6b733ea895fd47478957ed6c488},\\r\\nauthor_keywords={cell enzymes;  cell reaction;  macrophages;  mycobacterial polysaccharide;  phagocytosis},\\r\\npublisher={Kluwer Academic Publishers-Plenum Publishers},\\r\\nissn={00074888},\\r\\ncoden={BEXBA},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Bull Exp Biol Med},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Oleinikova, E.\",\"Egorov, N.\",\"Anoshkina, G.\",\"Morozov, A.\",\"Botvinko, I.\",\"Semenova, E.\"],\"key\":\"Oleinikova1983320\",\"id\":\"Oleinikova1983320\",\"bibbaseid\":\"oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&doi=10.1007%2fBF00830178&partnerID=40&md5=2d05f6b733ea895fd47478957ed6c488\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noskin\"],\"firstnames\":[\"L.A.\"],\"suffixes\":[]}],\"title\":\"Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents\",\"journal\":\"Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis\",\"year\":\"1983\",\"volume\":\"110\",\"number\":\"2\",\"pages\":\"393-399\",\"doi\":\"10.1016/0027-5107(83)90155-0\",\"note\":\"cited By 6\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&doi=10.1016%2f0027-5107%2883%2990155-0&partnerID=40&md5=618e32c711de3df565c57dec8399c8d5\",\"affiliation\":\"Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation\",\"abstract\":\"Inhibitors of DNA synthesis 1-β-arabinofuranosylcytosine (Ac) and hydroxyurea (Hu) taken together drastically sensitized human cells to the killing effect of DNA-damaging agents. For UV-irradiation this sensitization depended on the cells′ ability for excision repair. By using viscoelastometric methods of measurement of double-strand breaks (DSB) in the genome, it was established that the first DSB were generated after incubation of the damaged cells in the mixture of inhibitors at about the same dose when sensitization appeared. A scheme is proposed to described molecular events associated with the phenomenon studied. © 1983.\",\"correspondence_address1\":\"Filatov, M.V.; Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation\",\"issn\":\"00275107\",\"coden\":\"MRFME\",\"pubmed_id\":\"6877262\",\"language\":\"English\",\"abbrev_source_title\":\"Mutat. Res. Fundam. Mol. Mech. Mutagen.\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1983393,\\r\\nauthor={Filatov, M.V. and Noskin, L.A.},\\r\\ntitle={Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents},\\r\\njournal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},\\r\\nyear={1983},\\r\\nvolume={110},\\r\\nnumber={2},\\r\\npages={393-399},\\r\\ndoi={10.1016/0027-5107(83)90155-0},\\r\\nnote={cited By 6},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&doi=10.1016%2f0027-5107%2883%2990155-0&partnerID=40&md5=618e32c711de3df565c57dec8399c8d5},\\r\\naffiliation={Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},\\r\\nabstract={Inhibitors of DNA synthesis 1-β-arabinofuranosylcytosine (Ac) and hydroxyurea (Hu) taken together drastically sensitized human cells to the killing effect of DNA-damaging agents. For UV-irradiation this sensitization depended on the cells′ ability for excision repair. By using viscoelastometric methods of measurement of double-strand breaks (DSB) in the genome, it was established that the first DSB were generated after incubation of the damaged cells in the mixture of inhibitors at about the same dose when sensitization appeared. A scheme is proposed to described molecular events associated with the phenomenon studied. © 1983.},\\r\\ncorrespondence_address1={Filatov, M.V.; Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},\\r\\nissn={00275107},\\r\\ncoden={MRFME},\\r\\npubmed_id={6877262},\\r\\nlanguage={English},\\r\\nabbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Noskin, L.\"],\"key\":\"Filatov1983393\",\"id\":\"Filatov1983393\",\"bibbaseid\":\"filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&doi=10.1016%2f0027-5107%2883%2990155-0&partnerID=40&md5=618e32c711de3df565c57dec8399c8d5\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Belyakova\"],\"firstnames\":[\"N.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Naryzhnyi\"],\"firstnames\":[\"S.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Krutyakov\"],\"firstnames\":[\"V.M.\"],\"suffixes\":[]}],\"title\":\"Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin\",\"journal\":\"Radiobiologiya\",\"year\":\"1982\",\"volume\":\"22\",\"number\":\"5\",\"pages\":\"593-596\",\"note\":\"cited By 1\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&partnerID=40&md5=e6f60aadab293185ae5595d6bf4bebdf\",\"affiliation\":\"B.P. Konstantinov Leningrad Inst. Nucl. Phys., USSR Acad. Sci., Gatchina\",\"abstract\":\"Analysis was made of the enzymic and structural factors responsible for activation of repair DNA synthesis in γ-irradiated chromatin isolated from rat liver or some human cells. The results obtained prompted us to reduce by 10-12 times the dose of radiation used before. With doses of 30 Gy and 10 Gy, the value of the original repair synthesis was doubled in the chromatin of rat liver and HeLa cells, respectively.\",\"issn\":\"00338192\",\"coden\":\"RADOA\",\"pubmed_id\":\"6294723\",\"language\":\"Russian\",\"abbrev_source_title\":\"RADIOBIOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Belyakova1982593,\\r\\nauthor={Belyakova, N.V. and Naryzhnyi, S.N. and Filatov, M.V. and Krutyakov, V.M.},\\r\\ntitle={Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin},\\r\\njournal={Radiobiologiya},\\r\\nyear={1982},\\r\\nvolume={22},\\r\\nnumber={5},\\r\\npages={593-596},\\r\\nnote={cited By 1},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&partnerID=40&md5=e6f60aadab293185ae5595d6bf4bebdf},\\r\\naffiliation={B.P. Konstantinov Leningrad Inst. Nucl. Phys., USSR Acad. Sci., Gatchina},\\r\\nabstract={Analysis was made of the enzymic and structural factors responsible for activation of repair DNA synthesis in γ-irradiated chromatin isolated from rat liver or some human cells. The results obtained prompted us to reduce by 10-12 times the dose of radiation used before. With doses of 30 Gy and 10 Gy, the value of the original repair synthesis was doubled in the chromatin of rat liver and HeLa cells, respectively.},\\r\\nissn={00338192},\\r\\ncoden={RADOA},\\r\\npubmed_id={6294723},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={RADIOBIOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Belyakova, N.\",\"Naryzhnyi, S.\",\"Filatov, M.\",\"Krutyakov, V.\"],\"key\":\"Belyakova1982593\",\"id\":\"Belyakova1982593\",\"bibbaseid\":\"belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&partnerID=40&md5=e6f60aadab293185ae5595d6bf4bebdf\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Botvinko\"],\"firstnames\":[\"I.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Grechushkina\"],\"firstnames\":[\"N.N.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Semenova\"],\"firstnames\":[\"E.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Egorov\"],\"firstnames\":[\"N.S.\"],\"suffixes\":[]}],\"title\":\"Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.]\",\"journal\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"year\":\"1981\",\"number\":\"4\",\"pages\":\"95-99\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&partnerID=40&md5=2e1d7f3a9923aa76ca56ba13cbc0989d\",\"correspondence_address1\":\"Botvinko, I.V.\",\"issn\":\"04704606\",\"pubmed_id\":\"7236813\",\"language\":\"Russian\",\"abbrev_source_title\":\"Nauchnye Doki Vyss Shkoly Biol Nauki\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Botvinko198195,\\r\\nauthor={Botvinko, I.V. and Grechushkina, N.N. and Semenova, E.V. and Egorov, N.S.},\\r\\ntitle={Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.]},\\r\\njournal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},\\r\\nyear={1981},\\r\\nnumber={4},\\r\\npages={95-99},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&partnerID=40&md5=2e1d7f3a9923aa76ca56ba13cbc0989d},\\r\\ncorrespondence_address1={Botvinko, I.V.},\\r\\nissn={04704606},\\r\\npubmed_id={7236813},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\\r\\n\",\"author_short\":[\"Botvinko, I.\",\"Grechushkina, N.\",\"Semenova, E.\",\"Egorov, N.\"],\"key\":\"Botvinko198195\",\"id\":\"Botvinko198195\",\"bibbaseid\":\"botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&partnerID=40&md5=2e1d7f3a9923aa76ca56ba13cbc0989d\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Filatov\"],\"firstnames\":[\"M.V.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Sheikina\"],\"firstnames\":[\"T.A.\"],\"suffixes\":[]}],\"title\":\"Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation\",\"journal\":\"Radiobiologiya\",\"year\":\"1978\",\"volume\":\"18\",\"number\":\"5\",\"pages\":\"751-754\",\"note\":\"cited By 0\",\"url\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&partnerID=40&md5=4218fc5755a7f1beb190156f709214c7\",\"affiliation\":\"B.P. Konstantinov Inst. Nucl. Phys., USSR Acad. Sci., Leningrad\",\"abstract\":\"Interphase death of HeLa cells was assessed by the ability of cells to be stained with trypan blue after γ-irradiation with doses from 50 to 2000 krad. A quantitative relationship between average life span of cells and radiation dose has been shown. A decrease of the postirradiation temperature and an increase of the serum content in the growth medium before irradiation have been found to delay the interphase death. Adhesive properties of cells were damaged immediately after exposure.\",\"issn\":\"00338192\",\"coden\":\"RADOA\",\"pubmed_id\":\"715202\",\"language\":\"Russian\",\"abbrev_source_title\":\"RADIOBIOLOGIYA\",\"document_type\":\"Article\",\"source\":\"Scopus\",\"bibtex\":\"@ARTICLE{Filatov1978751,\\r\\nauthor={Filatov, M.V. and Sheikina, T.A.},\\r\\ntitle={Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation},\\r\\njournal={Radiobiologiya},\\r\\nyear={1978},\\r\\nvolume={18},\\r\\nnumber={5},\\r\\npages={751-754},\\r\\nnote={cited By 0},\\r\\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&partnerID=40&md5=4218fc5755a7f1beb190156f709214c7},\\r\\naffiliation={B.P. Konstantinov Inst. Nucl. Phys., USSR Acad. Sci., Leningrad},\\r\\nabstract={Interphase death of HeLa cells was assessed by the ability of cells to be stained with trypan blue after γ-irradiation with doses from 50 to 2000 krad. A quantitative relationship between average life span of cells and radiation dose has been shown. A decrease of the postirradiation temperature and an increase of the serum content in the growth medium before irradiation have been found to delay the interphase death. Adhesive properties of cells were damaged immediately after exposure.},\\r\\nissn={00338192},\\r\\ncoden={RADOA},\\r\\npubmed_id={715202},\\r\\nlanguage={Russian},\\r\\nabbrev_source_title={RADIOBIOLOGIYA},\\r\\ndocument_type={Article},\\r\\nsource={Scopus},\\r\\n}\\r\\n\",\"author_short\":[\"Filatov, M.\",\"Sheikina, T.\"],\"key\":\"Filatov1978751\",\"id\":\"Filatov1978751\",\"bibbaseid\":\"filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&partnerID=40&md5=4218fc5755a7f1beb190156f709214c7\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":0,\"html\":\"\"}],\n      metadata: {\"authorlinks\":{}},\n      ownerID: undefined\n    };\n  </script>\n\n  <script type=\"text/javascript\">\n  var site$;\n  if (typeof $ != 'undefined') {\n    console.log('existing jquery: storing and then restoring');\n    site$ = $;\n    $ = null;\n  }\n  </script>\n\n  <script src=\"https://ajax.googleapis.com/ajax/libs/jquery/2.2.4/jquery.min.js\">\n  </script>\n  <script type=\"text/javascript\">\n  if (site$) {\n    console.log('existing jquery: avoiding conflict and restoring');\n    bb$ = $.noConflict(true);\n    $ = site$;\n  } else {\n    bb$ = $;\n  }\n  </script>\n\n  <script src=\"//bibbase.org/js/bibbase.min.js\"\n          type=\"text/javascript\">\n  </script>\n\n  <script type=\"text/javascript\"\n          src=\"https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML\">\n  </script>\n  <script type=\"text/x-mathjax-config\">\n    MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\\\(','\\\\)']]},\n                        skipTags: [\"body\"],\n                        processClass: \"bibbase_paper\"});\n  </script>\n\n  <style>\n  @media print {\n    .dontprint {\n        display: none !important;\n    }\n\n  .bibbase_group {\n    background-color: #E0E0E0;\n    font-style: italic;\n    font-size: larger;\n    text-shadow: 0px 0px 3px #FFFFFF;\n    border-radius: 4px 4px 4px 4px;\n    -moz-border-radius: 4px 4px 4px 4px;\n    -webkit-border-radius: 4px;\n    padding: 5px 40px 5px;\n    margin-top: 10px;\n    margin-bottom: 5px;\n    cursor: pointer;\n  }\n\n  .bibbase_paper {\n    margin-bottom: 5px;\n  }\n\n  }\n  </style>\n\n  \n  <div style=\"float: right; margin-right: 10px; margin-top: 10px; text-align: right; font-size: 12px\">\n    generated by\n    <a href=\"//bibbase.org\"\n       onclick=\"trackOutboundLink('bibbase', 'logo clicked', window.location.href, '//bibbase.org'); return false;\">\n      <img src=\"//bibbase.org/img/logo.svg\"\n           style=\"vertical-align: middle; margin-left: 3px; height: 16px;\"/\n           alt=\"bibbase.org\"\n           title=\"BibBase – The easiest way to maintain your publications page.\"\n        ></a>\n    \n  </div>\n  \n\n  <div id=\"bibbase_header\" class=\"dontprint\" style=\"\">\n\n    <ul class=\"nav nav-pills\" style=\"margin: 0px;\">\n\n      <li class=\"dropdown\" id=\"groupby_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\">\n          Group by\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li class=\"groupby year\"><a onclick=\"groupby('year')\">\n            Year</a>\n        </li>\n        <li class=\"groupby author\"><a onclick=\"groupby('author_short')\">\n            Author</a>\n        </li>\n        <li class=\"groupby type\"><a onclick=\"groupby('type')\">\n            Type</a>\n        </li>\n        <li class=\"groupby keyword\"><a onclick=\"groupby('keyword')\">\n            Keyword</a>\n        </li>\n        <li class=\"groupby downloads\"><a onclick=\"groupby('downloads')\">\n            Downloads</a>\n        </li>\n      </ul>\n      </li>\n\n\n      <li class=\"dropdown\" id=\"menu_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\" alt=\"controls\">\n          <i class=\"fa fa-reorder\" alt=\"controls\"></i>\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li>\n          <a onclick=\"toggleAll()\">\n            <i class=\"fa fa-chevron-down fa-fw\"></i> Expand/Collapse All\n          </a>\n        </li>\n        <li>\n          <a download=\"lkb_2019_10.txt\" href=\"data:text/bibtex;base64,





@ARTICLE{Pantina2019188,
author={Pantina, R.A. and Varfolomeeva, E.Y. and Burdakov, V.S. and Landa, S.B. and Bayramukov, V.Y. and Kovalev, R.A. and Filatov, M.V.},
title={Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells},
journal={Cell and Tissue Biology},
year={2019},
volume={13},
number={3},
pages={188-197},
doi={10.1134/S1990519X1903009X},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&doi=10.1134%2fS1990519X1903009X&partnerID=40&md5=89a5f8a9f3aba34d2c3fe326d4ff81c1},
affiliation={St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, 191036, Russian Federation},
abstract={Abstract: In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population. © 2019, Pleiades Publishing, Ltd.},
author_keywords={cell-to-cell communication;  exosomes;  Hek293 cell line;  p53-GFP},
correspondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov InstituteRussian Federation; email: fil_53@mail.ru},
publisher={Pleiades Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Volnitskiy2019,
author={Volnitskiy, A. and Shtam, T. and Burdakov, V. and Kovalev, R. and Konev, A. and Filatov, M.},
title={Abnormal activity of transcription factors gli in high-grade gliomas},
journal={PLoS ONE},
year={2019},
volume={14},
number={2},
doi={10.1371/journal.pone.0211980},
art_number={e0211980},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&doi=10.1371%2fjournal.pone.0211980&partnerID=40&md5=b33be3ba1c58420838f9b1c65e11c6ae},
affiliation={Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre "Kurchatov Institute, Gatchina, Russian Federation; N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Pesochnyj, Leningradskaya, Russian Federation},
abstract={Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity of transcription factors gli in high-grade gliomas and their role in maintenance of stem cell state and glioma cell survival. 20 glioma cell lines and a sample of a normal adult brain tissue were used in the present study. We found the expression of gli target genes, including GLI1 and FOXM1, in all tested glioma cell lines, but not in the normal tissue. Interestingly, the expression of gli target genes in some glioma cell lines was observed together with a high level of their transcriptional repressor, Gli3R. Knockdown of GLI3 in one of these lines resulted in decrease of gli target gene expression. These data suggest that Gli3R does not prevent the gli target genes transcription, and gli3 acts in glioma cells more as an activator, than a repressor of transcription. We observed that gli regulated the expression of such genes, as SOX2 or OCT4 that maintain stem cell state, and TET1, involving in DNA demethylation. Treatment with GANT61 or siRNA against GLI1, GLI2, or GLI3 could result in complete glioma cell death, while cyclopamine had a weaker and line-specific effect on glioma cell survival. Thus, the gli transcription factors are abnormally active in high-grade gliomas, regulate expression of genes, maintaining the stem cell state, and contribute to glioma cell survival. © 2019 Volnitskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
correspondence_address1={Volnitskiy, A.; Petersburg Nuclear Physics Institute named by B.P. Konstantinov, National Research Centre "Kurchatov InstituteRussian Federation; email: voln.a@yandex.ru},
publisher={Public Library of Science},
issn={19326203},
coden={POLNC},
pubmed_id={30730955},
language={English},
abbrev_source_title={PLoS ONE},
document_type={Article},
source={Scopus},
}



@ARTICLE{Zinchenko2019479,
author={Zinchenko, Yu.S. and Starshinova, A.A. and Filatov, M.V. and Denisova, N.V. and Landa, S.B. and Burdakov, V.S. and Yablonskiy, P.K.},
title={Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ]},
journal={Medical Immunology (Russia)},
year={2019},
volume={21},
number={3},
pages={479-486},
doi={10.15789/1563-0625-2019-3-479-486},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&doi=10.15789%2f1563-0625-2019-3-479-486&partnerID=40&md5=3491db5f8ebdda1e86d4978600a5e9e7},
affiliation={St. Petersburg State Research Institute of Phthisiopulmonology, St. Petersburg, Russian Federation; St. Petersburg B. Konstantinov Institute of Nuclear Physics, National Research Center “Kurchatov Institute, Gatchina, Leningrad Region, Russian Federation; St. Petersburg State University, St. Petersburg, Russian Federation},
abstract={The etiology of sarcoidosis is not completely understood. A hypothesis exists about the relationship between sarcoidosis and a complex of pathological autoimmune reactions that occur under the influence of triggering factors. In this study, specific immune complexes in the blood plasma of patients have been determined, which can indirectly reveal the causes of the disease. The study included 33 patients with lung sarcoidosis (I group), compared to 24 healthy donors who served as a control group (II group). The patients underwent standard examination. Their blood plasma was investigated by the dynamic light scattering method with addition of tuberculosis antigens (ESAT-6/SFP-10) and “lung healthy tissue extract”. Statistical analysis was performed using the Statistica 7.0 program. Test results were considered significant at p < 0.05. Аccording to the data obtained, addition of ESAT-6/SFP-10 to patient’s blood plasma almost did not lead to the formation of immune complexes in most samples. Meanwhile, development of such complexes after addition of “lung tissue extract” was revealed in all the patients. The immune complexes were not detected in any donor from control group after stimulation with both kinds of antigens (p < 0.01). The data on distinct formation of immune complexes with the addition of “lung healthy tissue extract” in patients with lung sarcoidosis may be considered an indirect evidence for occurrence of autoimmune reaction under the influence of some pathogenic factors. Absence of de novo immune complex formation after addition of tuberculosis antigens (ESAT-6/SFP-10) makes it unlikely any direct effects of tuberculosis bacteria upon development of sarcoidosis. © 2019, SPb RAACI.},
author_keywords={Autoimmunity;  Dynamic light scattering method;  Sarcoidosis;  Specific immune complexes},
correspondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},
publisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},
issn={15630625},
language={Russian},
abbrev_source_title={Med. Immunol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Starshinova2019269,
author={Starshinova, A.A. and Istomina, E.V. and Zinchenko, Yu.S. and Filatov, M.V. and Landa, S.B. and Burdakov, V.S. and Belyaeva, E.N. and Nazarenko, M.M. and Sapozhnikova, N.V. and Yablonkiy, P.K.},
title={Diagnostic value of specific immune complexes in detection of active tuberculosis infection},
journal={Medical Immunology (Russia)},
year={2019},
volume={21},
number={2},
pages={269-278},
doi={10.15789/1563-0625-2019-2-269-278},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&doi=10.15789%2f1563-0625-2019-2-269-278&partnerID=40&md5=a90c16d4007b2884eb6918641062312c},
affiliation={St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, St. Petersburg, 191036, Russian Federation},
abstract={The diagnostics of tuberculosis infection, including immunological methods, evolved significant changes over time. Introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to differentiate between these conditions, thus requiring development and implementation of new diagnostic approaches. A prospective study enrolling two groups of patients was conducted as follows: group I (n = 50) included patients with verified pulmonary tuberculosis, MBT (+), whereas group II (n = 15) consisted of subjects with LTI, and control group was represented by healthy subjects (n = 14). The entire examination protocol included clinical, radiological, bacteriological, and immunological methods (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskin test). Immune complexes were determined in all patients and healthy individuals by means of dynamic light scattering after the in vitro addition of specific antigens (ESAT-6 and SFP-10 peptides). The data obtained have shown low informativity of clinical methods in diagnostics of pulmonary tuberculosis. In the presence of characteristic X-radiographic changes, bacteriological verification of tuberculosis was proven only in 46% of cases. Usage of various immunological tests allowed to obtain positive results in 84-90% of cases, along with 100% positivity in subjects with LTI. Detection of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases, and to identify the high-risk group for development of active tuberculosis among the subjects with latent tuberculosis infection. Conclusions: the obtained data may be applied both for diagnosis of active tuberculosis in the absence of verified diagnosis, but also enable identification of a high-risk group for development of the disease in subjects with latent tuberculosis infection. © 2019, SPb RAACI.},
author_keywords={Dynamic light scattering method;  Immunological tests;  Latent tuberculosis infection;  Test with allergen tuberculosis recombinant;  Tuberculosis},
correspondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},
publisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},
issn={15630625},
language={Russian},
abbrev_source_title={Med. Immunol.},
document_type={Article},
source={Scopus},
}







@ARTICLE{Chernous2018562,
author={Chernous, S.A. and Shagimuratov, I.I. and Ievenko, I.B. and Filatov, M.V. and Efishov, I.I. and Shvets, M.V. and Kalitenkov, N.V.},
title={Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals},
journal={Russian Journal of Physical Chemistry B},
year={2018},
volume={12},
number={3},
pages={562-567},
doi={10.1134/S1990793118030065},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&doi=10.1134%2fS1990793118030065&partnerID=40&md5=bef4391cf3e6455e8b4c238f9b0e484a},
affiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Kaliningrad Branch, Russian Academy of Sciences, Kaliningrad, 236017, Russian Federation; Institute for Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677098, Russian Federation; Murmansk State Technical University, Sportivnaya 13, Murmansk, 183010, Russian Federation; Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russian Federation},
abstract={A comparative analysis of total electron content (TEC) fluctuations and auroral activity, which characterizes the polar ionosphere during periods of substorm activity, is performed. The analysis is based on GPS/GLONASS observations at auroral and subauroral stations and data on auroral emissions obtained at the Yakutsk subauroral station and the Poker Flat (Alaska) auroral station. A detailed analysis is carried out for the storm on January 7, 2015. During this event, a sharp increase in the auroral activity and in TEC fluctuations from 09 to 13 UT was observed. A similarity of the dynamics of the auroral oval and the space–time distribution of TEC fluctuations associated with ionospheric irregularities in the oval is demonstrated. There is a close correlation between the positions of the auroral oval and the irregularity oval. The positions of the auroral oval, predicted by the NORUSKA Russian–Norwegian model, and of the TEC irregularity oval are compared. In general, the positions of the ovals are close to each other. The existing discrepancy may be due to the fact that the auroral oval is projected to an altitude of 110 km, whereas the irregularity oval, to an altitude of 450 km, and that the curvature of the magnetic force lines is not taken into account. The influence of auroral disturbances on navigational measurements manifests itself through the fact that, during disturbances, faults and failures in the operation of navigation equipment at auroral and subauroral stations increase. © 2018, Pleiades Publishing, Ltd.},
author_keywords={auroral oval;  auroral perturbations;  GPS/GLONASS;  TEC fluctuations;  TEC irregularity oval},
correspondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of SciencesRussian Federation; email: chernouss@pgia.ru},
publisher={Pleiades Publishing},
issn={19907931},
language={English},
abbrev_source_title={Russ. J. Phys. Chem. B},
document_type={Article},
source={Scopus},
}



@ARTICLE{Burdakov201820,
author={Burdakov, V.S. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Filatov, M.V.},
title={Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells},
journal={Cell and Tissue Biology},
year={2018},
volume={12},
number={1},
pages={20-26},
doi={10.1134/S1990519X18010030},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&doi=10.1134%2fS1990519X18010030&partnerID=40&md5=f9de8e8533842e75b6479cbae6afc729},
affiliation={Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; College of Health and Life Sciences, Brunel University, London, Uxbridge, United Kingdom; St. Petersburg Institute of Phthisiopulmonology, St. Petersburg, 191036, Russian Federation},
abstract={Exosomes are nanosized vesicles that are secreted by many types of cells. We have found that exosomes secreted by HEK293 and HT-1080 can suppress growth and proliferation of p53-deficient cells. Upon overexpression of exogenous p53-GFP in HEK293 cells, we observed p53 protein in exosomes that were secreted by these cells. We also found endogenous p53 in exosomes that were secreted by HT-1080 cells with a higher level of p53 expression. We were able to detect endogenous p53 protein in exosomes that originated from human plasma and were transferred to p53-deficient cells. Our findings indicate that p53 protein can be transferred between cells and may play an important physiological role. © 2018, Pleiades Publishing, Ltd.},
author_keywords={cell lines;  exosomes;  р53},
correspondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”Russian Federation; email: fil_53@mail.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Belyakova201839,
author={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},
title={Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same},
journal={Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry},
year={2018},
volume={12},
number={1},
pages={39-42},
doi={10.1134/S199075081801002X},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&doi=10.1134%2fS199075081801002X&partnerID=40&md5=5c507aecac55632ff5640f00b0f7139c},
affiliation={Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov Institute, Leningrad region, Gatchina, 188300, Russian Federation},
abstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-toback principle. Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well. © 2018, Pleiades Publishing, Ltd.},
author_keywords={cytoplasm;  double trimer;  monomer;  neutrophil;  PCNA;  structure},
correspondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov InstituteRussian Federation; email: snaryzhny@mail.ru},
publisher={Pleiades Publishing},
issn={19907508},
language={English},
abbrev_source_title={Biochem. (Moscow) Suppl. Ser. B Biomed. Chem.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov2018,
author={Filatov, M.V. and Landa, S.B. and Korabliov, P.V. and Semenova, E.V.},
title={Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations]},
journal={F1000Research},
year={2018},
volume={7},
doi={10.12688/f1000research.14406.1},
art_number={618},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&doi=10.12688%2ff1000research.14406.1&partnerID=40&md5=5cdb17d6a9583e9bc8443ee0ec1012f7},
affiliation={Division of Molecular and Radiation Biophysics, National Research Center, Gatchina, 188300, Russian Federation; State Research Institute Center for Innovative Medicine, Vilnius, LT-01102, Lithuania; Saint Petersburg State Res Inst of Phthisiopulmonology of the Min of Healthcare of the Russian Fed, Saint Petersburg, 191036, Russian Federation},
abstract={Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body's immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7-8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular-cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely. © 2018 Landa SB et al.},
author_keywords={Digestion;  Dynamic light scattering method;  irculating immune complexes;  Isotypes of immunoglobulins},
correspondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, National Research CenterRussian Federation; email: fil_53@mail.ru},
publisher={F1000 Research Ltd},
issn={20461402},
language={English},
abbrev_source_title={F1000 Res.},
document_type={Article},
source={Scopus},
}





@ARTICLE{Petrenko2018519,
author={Petrenko, E.S. and Kopylov, A.T. and Kleist, O.A. and Legina, O.K. and Belyakova, N.V. and Pantina, R.A. and Naryzhny, S.N.},
title={In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells},
journal={Tsitologiya},
year={2018},
volume={60},
number={7},
pages={519-523},
doi={10.31116/tsitol.2018.07.05},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&doi=10.31116%2ftsitol.2018.07.05&partnerID=40&md5=4fb79a48075054ea3847204606cafb5e},
affiliation={V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russian Federation; B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre Kurchatov Institute, Gatchina, Leningrad Region, 188300, Russian Federation},
abstract={Proteins from normal (fibroblasts) and cancer (glioblastoma) human cells were separated by two dimensional gel electrophoresis. Next, a sectional analysis of these gels by mass spectrometry was performed, and profiles of proteoforms coded by more than 5000 genes were obtained. It was found that some proteins have specific for glioblastoma cells proteoforms. We assume that these proteoforms could be used as specific for glioblastoma biomarkers. © 2018 Sankt Peterburg. All rights reserved.},
author_keywords={Biomarker;  Glioblastoma;  Mass spectrometry;  Proteoforma;  Proteome;  Two dimensional electrophoresis},
correspondence_address1={Naryzhny, S.N.; V. N. Orekhovich Research Institute of Biomedical ChemistryRussian Federation; email: naryzhnyy_sn@pnpi.nrcki.ru},
publisher={Sankt Peterburg},
issn={00413771},
coden={TSITA},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Kiseleva2018,
author={Kiseleva, L.N. and Kartashev, A.V. and Vartanyan, N.L. and Pinevich, A.A. and Filatov, M.V. and Samoilovich, M.P.},
title={Characterization of New Human Glioblastoma Cell Lines},
journal={Cell and Tissue Biology},
year={2018},
volume={12},
number={1},
doi={10.1134/S1990519X18010108},
note={cited By 5},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&doi=10.1134%2fS1990519X18010108&partnerID=40&md5=c3954c3ded80c915486fe0c5d05ab388},
affiliation={Russian Research Center for Radiology and Surgical Technologies, St. Petersburg, 197758, Russian Federation; St. Petersburg State University, St. Petersburg, 199034, Russian Federation; Northwestern State Medical University named after I.I. Mechnikov, St. Petersburg, 191015, Russian Federation; St. Petersburg Nuclear Physics Institute named after B.P. Konstantinov, St. Petersburg, 188300, Russian Federation},
abstract={Glioblastomas are tumors of neuroectodermal origin with a high degree of diversity. Tumor cells isolated from the surgical material of patients with glioblastomas are heterogeneous populations varying in morphology, phenotype, and genetic characteristics. This paper presents a description of two new glioblastoma cell lines, R1 and T2, isolated from tumor tissue of patients in 2010. We investigated their morphological and cytochemical cell characteristics and the expression of neuronal, mesenchymal, and endothelial markers, as well as the activity of genes encoding a number of growth factors, extracellular matrix proteins, and intracellular proteins typical for cells of mesenchymal origin. R1 and T2 cell lines are morphologically different. T2 cell line was characterized by the presence of multinuclear cells. In terms of the expression of β-tubulin III, MGMT, and p53 protein, R1 cell line was more heterogeneous than T2. R1 and T2 glioblastoma cell lines also differed in the presence and ratio of cell populations with mesenchymal, neuronal, and endothelial markers. Thus, neuronal markers CD133/2 and CD56 were detected only on R1 cells. Both lines were characterized by high activity of growth factor genes TGFβ1, VEGF, and FGF2(b), lower activity of EGF, and high expression of THBS1 and αSMA genes. However, the activity of most of the genes under study in R1 cells was higher than in T2 cells. The greatest difference lay in the expression of HGF, FAP, and TNC. Comparison of two new glioblastoma cell lines, R1 and T2, with the continuously cultivated lines А172 and T98G showed that R1 line had remarkable similarity with A172, while T2 glioblastoma resembled T98G cells. Apparently, the differences between R1 and T2 cell lines are determined by the properties of the initial tumors rather than the time of the cell cultivation. © 2018, Pleiades Publishing, Ltd.},
author_keywords={A172;  gene expression;  glioblastoma cell lines;  growth factors;  surface markers;  T98G},
correspondence_address1={Kiseleva, L.N.; Russian Research Center for Radiology and Surgical TechnologiesRussian Federation; email: luba_kiseleva@mail.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Shtam2017,
author={Shtam, T. and Samsonov, R. and Kamyshinsky, R. and Pantina, R. and Verlov, N. and Vasiliev, A. and Konevega, A.L. and Malek, A.V.},
title={Exosomes: Some approaches to cancer diagnosis and therapy},
journal={AIP Conference Proceedings},
year={2017},
volume={1882},
doi={10.1063/1.5001645},
art_number={020066},
note={cited By 5; Conference of International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference Date: 23 May 2017 Through 26 May 2017;  Conference Code:133914},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&doi=10.1063%2f1.5001645&partnerID=40&md5=d908b66243d4ade3a27c45bad492f886},
affiliation={Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov Institute, Gatchina, Russian Federation; Petrov Institute of Oncology, Saint-Petersburg, Russian Federation; Ltd. Oncosystem, Skolkovo, Russian Federation; Peter the Great Saint-Petersburg Polytechnic University, St. Petersburg, Russian Federation; National Research Center, Kurchatov Institute, Moscow, Russian Federation},
abstract={Exosomes are membrane-bound, intercellular communication shuttle vesicles that are defined by their endocytic origin and size range of 30-120 nm. Secreted by nearly all mammalian cell types and present in bodily fluids, exosomes confer messages between cells, by transporting functionally relevant proteins, nucleic acids, and lipids. The capability of tumor exosomes to house tumorigenic information and induce cellular responses that promote disease pathogenesis make tumor exosomes an attractive tool in identifying cancer biomarkers and exploiting exosomes for therapy. In this paper, we sum up our previous findings to utilize exosomes as biomarkers for early detection, diagnosis and therapy selection of prostate and thyroid cancer and present our results on exosomes in colon cancer. Some of plasma exosomal miRNAs showed their potential as diagnostic markers for colon cancer. All together, the data suggested the potentials of circulating exosomal miRNAs as liquid biopsy markers for cancer. Here we also present the possibilities of delivering therapeutic molecules by exosomes. Previously, we had demonstrated the potential of exosome-mediated siRNA delivery. Here, we present the possibility of carrying the exogenous p53 protein by exosomes in vitro. © 2017 Author(s).},
correspondence_address1={Shtam, T.; Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov InstituteRussian Federation; email: tatyana_shtam@mail.ru},
editor={Gutmanas E.Y., Naimark O.B., Sharkeev Y.P.},
sponsors={Institute of Continuous Media Mechanics of UrB RAS; Institute of Strength Physics and Materials Science of SB RAS; National Research Tomsk Polytechnic University; National Research Tomsk State University; Tomsk Research Institute of Oncology},
publisher={American Institute of Physics Inc.},
issn={0094243X},
isbn={9780735415621},
language={English},
abbrev_source_title={AIP Conf. Proc.},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Makarov2017,
author={Makarov, E.M. and Shtam, T.A. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Filatov, M.V.},
title={The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis},
journal={PLoS ONE},
year={2017},
volume={12},
number={9},
doi={10.1371/journal.pone.0185126},
art_number={e0185126},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&doi=10.1371%2fjournal.pone.0185126&partnerID=40&md5=e33272f5dbd1aae05eb33bbe72e9eea2},
affiliation={Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; Peter the Great St Petersburg Polytechnic University, St Petersburg, Russian Federation; Petrov Institute of Oncology, St Petersburg, Russian Federation},
abstract={P53 protein is more frequently mutated in human tumours compared with the other proteins. While the majority of the p53 mutations, especially within its DNA-binding domain, lead to the loss of the wild-type function, there are accumulating data demonstrating that the p53 mutants gain tumour promoting activities; the latter triggers a revitalised interest in functional analysis of the p53 mutants. A systematic screening for p53 mutations in surgical materials from patients with glioma revealed a 378C>G mutation that creates a stop codon at the position of amino acid residue 126. The mutation eliminates the recognition site for the restriction endonuclease Sca I that allowed us to carry out RFLP analysis of DNA extracted from the clinical samples and suggests that this mutation is more frequent than is documented in the p53 databases. Both the ECV-304 and EJ cell lines, that probably originate from the bladder carcinoma T24 cell line, were confirmed to contain the homozygous 378C>G mutation but were shown to produce the p53 protein of expected full-length size detected by Western blotting. We provide evidence that the 378C>G mutation generates an alternative 3’ splice site (ss) which is more often used instead of the authentic upstream 3’ ss, driving the production of mRNA encoding the protein with the single amino acid deletion (p53ΔY126). Using endogenous expression, we demonstrated that the p53ΔY126 protein is nearly as active as the wild type protein in inducing the p21/Waf1 expression and apoptosis. © 2017 Makarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
correspondence_address1={Filatov, M.V.; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil53ster@gmail.com},
publisher={Public Library of Science},
issn={19326203},
coden={POLNC},
pubmed_id={28961258},
language={English},
abbrev_source_title={PLoS ONE},
document_type={Article},
source={Scopus},
}



@ARTICLE{Iashina2017,
author={Iashina, E.G. and Velichko, E.V. and Filatov, M.V. and Bouwman, W.G. and Duif, C.P. and Brulet, A. and Grigoriev, S.V.},
title={Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei},
journal={Physical Review E},
year={2017},
volume={96},
number={1},
doi={10.1103/PhysRevE.96.012411},
art_number={012411},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&doi=10.1103%2fPhysRevE.96.012411&partnerID=40&md5=e15ead8307ee2efbb140f35ce40ca971},
affiliation={Petersburg Nuclear Physics Institute, Gatchina, St.-Petersburg, 188300, Russian Federation; Saint Petersburg State University, Ulyanovskaya 1, St.-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, JB Delft, 2629, Netherlands; Leon Brillouin Laboratory, CEA Saclay, Gif sur Yvette Cedex, 91191, France},
abstract={Small-angle neutron scattering (SANS) on nuclei of chicken erythrocytes demonstrates the cubic dependence of the scattering intensity Q-3 in the range of momentum transfer Q 10-3-10-2nm-1. Independent spin-echo SANS measurements give the spin-echo function, which is well described by the exponential law in a range of sizes (3×102)-(3×104) nm. Both experimental dependences reflect the nature of the structural organization of chromatin in the nucleus of a living cell, which corresponds to the correlation function γ(r)=ln(ξ/r) for r<ξ, where ξ=(3.69±0.07)×103 nm, the size of the nucleus. It has the specific scaling property of the logarithmic fractal γ(r/a)=γ(r)+ln(a), i.e., the scaling down by a gives an additive constant to the correlation function, which distinguishes it from the mass fractal, which is characterized by multiplicative constant. © 2017 American Physical Society.},
publisher={American Physical Society},
issn={24700045},
pubmed_id={29347273},
language={English},
abbrev_source_title={Phys. Rev. E},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Iashina2017,
author={Iashina, E.G. and Bouwman, W.G. and Duif, C.P. and Filatov, M.V. and Grigoriev, S.V.},
title={Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell},
journal={Journal of Physics: Conference Series},
year={2017},
volume={862},
number={1},
doi={10.1088/1742-6596/862/1/012010},
art_number={012010},
note={cited By 0; Conference of 11th International Conference on Polarised Neutrons for Condensed Matter Investigations, PNCMI 2016 ; Conference Date: 4 July 2016 Through 7 July 2016;  Conference Code:128574},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&doi=10.1088%2f1742-6596%2f862%2f1%2f012010&partnerID=40&md5=33d0632e7d097bc7f05412a5d0883c38},
affiliation={Petersburg Nuclear Physics Institute NRC KI, Gatchina, St-Petersburg, 188300, Russian Federation; Saint-Petersburg State University, Saint-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, Delft, 2629 JB, Netherlands; Department of Physics, University of Bristol, Tyndalls Park Road, Bristol, BS8 1TS, United Kingdom},
abstract={Spin-echo small-angle scattering (SESANS) technique is a method to measure the structure of materials from nano- to micrmeter length scales. This method could be important for studying the packaging of DNA in the eukaryotic cell. We measured the SESANS function from chicken erythrocyte nuclei which is well fitted by the exponential function G(z) = exp(-z/ξ), where ξ is the correlation length of a nucleus (in experimental data ξ = 3, 3 μm). The exponential decay of G(z) corresponds to the logarithmic pair correlation function γ(r) = ln(ξ/r). As the sensitivity of the SESANS signal depends on the neutron wavelength, we propose the SESANS setup with the changeable wavelength in the range from 2 to 12 Å. Such option allows one to study in great detail the internal structure of the biological cell in the length scale from 10-2 μm to 10 μm. © Published under licence by IOP Publishing Ltd.},
sponsors={AIRBUS DS; et al.; ISIS; J-PARC; LLB; Swiss Neutronics},
publisher={Institute of Physics Publishing},
issn={17426588},
language={English},
abbrev_source_title={J. Phys. Conf. Ser.},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Shtam2017172,
author={Shtam, T.A. and Burdakov, V.S. and Landa, S.B. and Naryzhny, S.N. and Bairamukov, V.Y. and Malek, A.V. and Orlov, Y.N. and Filatov, M.V.},
title={Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies},
journal={Cell and Tissue Biology},
year={2017},
volume={11},
number={2},
pages={172-179},
doi={10.1134/S1990519X17020043},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&doi=10.1134%2fS1990519X17020043&partnerID=40&md5=f439cee2262ea4552aa9c299648a59e1},
affiliation={National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Peter the Great Polytechnic University, St. Petersburg, 195251, Russian Federation; Petrov Institute of Oncology, Ministry of Healthcare of the Russian Federation, St. Petersburg, 197758, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russian Federation},
abstract={Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.},
author_keywords={exosomes;  lectins;  methods of exosome isolation},
correspondence_address1={Filatov, M.V.; National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil_53@mail.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Belyakova2017356,
author={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},
title={Quaternary structures of human cytoplasmic and nuclear PCNA are the same},
journal={Biomeditsinskaya Khimiya},
year={2017},
volume={63},
number={4},
pages={356-360},
doi={10.18097/PBMC20176304356},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&doi=10.18097%2fPBMC20176304356&partnerID=40&md5=ce5cadb78677efafb3f40f9a9cf8706b},
affiliation={Petersburg Nuclear Physics Institute NRC Kurchatov Institute, Leningrad Region (PNPI), Gatchina, 188300, Russian Federation},
abstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-to-back principle (Naryzhny S.N. et al. (2005) J. Biol. Chem., 280, 13888). Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well.},
author_keywords={Cytoplasm;  Double trimer;  Monomer;  Neutrophil;  PCNA;  Structure},
publisher={Russian Academy of Medical Sciences},
issn={23106905},
pubmed_id={28862608},
language={Russian},
abbrev_source_title={Biomeditsinskaya Khim.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Samsonov201612011,
author={Samsonov, R. and Burdakov, V. and Shtam, T. and Radzhabovа, Z. and Vasilyev, D. and Tsyrlina, E. and Titov, S. and Ivanov, M. and Berstein, L. and Filatov, M. and Kolesnikov, N. and Gil-Henn, H. and Malek, A.},
title={Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer},
journal={Tumor Biology},
year={2016},
volume={37},
number={9},
pages={12011-12021},
doi={10.1007/s13277-016-5065-3},
note={cited By 29},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&doi=10.1007%2fs13277-016-5065-3&partnerID=40&md5=44202226962197cb45fd5d86662beabe},
affiliation={Oncosystem Ltd, Hoshimina 11/1-207, Saint-Petersburg, 194356, Russian Federation; NN Petrov Institute of Oncology, Leningradskaya 68, Saint-Petersburg, 197758, Russian Federation; FSBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg  188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, 195251, Russian Federation; Institute of Molecular and Cellular Biology SB RAS, Lavrentieva 8/2, Novosibirsk, 630090, Russian Federation; Faculty of Medicine in the Galilee, Bar-Ilan University, Henrietta Szold 8, Safed, 13100, Israel},
abstract={Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over the last few decades. As has been revealed by a number of studies, TC tissue’s micro-RNA (miRNA) profile may reflect histological features and the clinical behavior of tumor. However, alteration of the miRNA profile of plasma exosomes associated with TC development has to date not been explored. We isolated exosomes from plasma and assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, and western blotting. Next, we profiled cancer-associated miRNAs in plasma exosomes obtained from papillary TC patients, before and after surgical removal of the tumor. The diagnostic value of selected miRNAs was evaluated in a large cohort of patients displaying different statuses of thyroid nodule disease. MiRNA assessment was performed by RT-qPCR. In total, 60 patients with different types of thyroid nodal pathology were included in the study. Our results revealed that the development of papillary TC is associated with specific changes in exosomal miRNA profiles; this phenomenon can be used for differential diagnostics. MiRNA-31 was found to be over-represented in the plasma exosomes of patients with papillary TC vs. benign tumors, while miRNA-21 helped to distinguish between benign tumors and follicular TC. MiRNA-21 and MiRNA-181a-5p were found to be expressed reciprocally in the exosomes of patients with papillary and follicular TC, and their comparative assessment may help to distinguish between these types of TC with 100 % sensitivity and 77 % specificity. © 2016, International Society of Oncology and BioMarkers (ISOBM).},
author_keywords={Diagnostics;  Exosomes;  MicroRNA;  Thyroid cancer},
correspondence_address1={Malek, A.; Oncosystem Ltd, Hoshimina 11/1-207, Russian Federation; email: anastasia@malek.com},
publisher={Springer Netherlands},
issn={10104283},
coden={TUMBE},
pubmed_id={27164936},
language={English},
abbrev_source_title={Tumor Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Varfolomeeva2016909,
author={Varfolomeeva, E.Y. and Semenova, E.V. and Sokolov, A.V. and Aplin, K.D. and Timofeeva, K.E. and Vasilyev, V.B. and Filatov, M.V.},
title={Ceruloplasmin decreases respiratory burst reaction during pregnancy},
journal={Free Radical Research},
year={2016},
volume={50},
number={8},
pages={909-919},
doi={10.1080/10715762.2016.1197395},
note={cited By 8},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&doi=10.1080%2f10715762.2016.1197395&partnerID=40&md5=e0176bf5319caea3ccbca3344954e1b4},
affiliation={Division of Molecular and Radiation Biophysics, SFBI “Petersburg Nuclear Physics Institute” (National Research Center “Kurchatov Institute”), Gatchina, Russian Federation; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, St. Petersburg, Russian Federation; Chair of Fundamental Problems of Medicine, Saint-Petersburg State University, St. Petersburg, Russian Federation},
abstract={Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin – a copper-containing glycoprotein – is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy. © 2016 Informa UK Limited, trading as Taylor & Francis Group.},
author_keywords={Ceruloplasmin;  flow cytometry;  neutrophils;  pregnancy;  respiratory burst reaction},
correspondence_address1={Sokolov, A.V.; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, Acad. Pavlova str., 12, Russian Federation; email: biochemsokolov@gmail.com},
publisher={Taylor and Francis Ltd},
issn={10715762},
coden={FRARE},
pubmed_id={27266720},
language={English},
abbrev_source_title={Free Radic. Res.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova2016259,
author={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},
title={Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas},
journal={Cell and Tissue Biology},
year={2016},
volume={10},
number={4},
pages={259-263},
doi={10.1134/S1990519X16040118},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&doi=10.1134%2fS1990519X16040118&partnerID=40&md5=9aafccdeff858ecc1fc8db1b00051926},
affiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation},
abstract={Although progress has been made in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that glioma malignant transformation is accompanied by gradual loss of LGI1 gene expression. However, genetic defects underlying LGI1 inactivation are not recognized. In this work, we analyzed LGI1 gene expression in primary cultures of malignant gliomas and compared these data with posttranslational modifications of H3 histone, an epigenetic indicator of transcriptional activity. We showed the presence of an epigenetic marker of gene repression H3K9me3 near LGL1 transcription initiation site in most malignant gliomas (five out of six). No expression of LGI1 gene was registered in these gliomas. LGI1 expression was documented only in one glioma, and this tumor did not exhibit an association of LGI1 gene with H3K9me3 modification. Thus, we demonstrated for the first time a correlation between an LGI1 gene expression and epigenetic indicator H3K9met3 in malignant gliomas. An Н3К4ас marker of actively transcribed chromatin near LGI1 transcription initiation site was not found. These data suggest the inactivation of LGI1 gene through an epigenetic mechanism: a modified “histone code.” © 2016, Pleiades Publishing, Ltd.},
author_keywords={epigenetic regulation;  gliomas;  histone code;  LGI1 gene},
correspondence_address1={Semenova, E.V.; Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov InstituteRussian Federation; email: semenova_el.spb@mail.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova2016335,
author={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},
title={THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS},
journal={Tsitologiia},
year={2016},
volume={58},
number={5},
pages={335-339},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&partnerID=40&md5=9d291f5873458153cdec69d5434ac722},
abstract={Although there is a progress in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that process of malignant transformation of gliomas is accompanied by gradual loss of LGI1 gene expression. However, genetic defects causing LGI1 inactivation have not been revealed. In this paper, we have analyzed the LGI1 gene expression in primary cultures of malignant gliomas, and compared these data with epigenetic indicators of transcriptional activity — posttranslational H3 histone modifications. We have show the presence of an epigenetic marker of gene repression H3K9me3 near the site of LGI1 transcription initiation in most (5 from 6) studied gliomas. There was not LGI1 expression in these gliomas. Only one glioma showed LGI1 expression, and in this glioma there was no association of LGI1gene with H3K9me3 modification. Thus, we are the first to show a correlation between LGI1 gene expression and the epigenetic indicator H3K9met3 in malignant gliomas. Marker of actively transcribed chromatin Í3K4àñ have not been found in this area of the genome. The data obtained strongly suggest the possibility of gene LGL1 inactivation by epigenetic mechanism: modified «histone code».},
issn={00413771},
pubmed_id={30188624},
language={English; Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Samsonov201668,
author={Samsonov, R. and Shtam, T. and Burdakov, V. and Glotov, A. and Tsyrlina, E. and Berstein, L. and Nosov, A. and Evtushenko, V. and Filatov, M. and Malek, A.},
title={Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic},
journal={Prostate},
year={2016},
volume={76},
number={1},
pages={68-79},
doi={10.1002/pros.23101},
note={cited By 62},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&doi=10.1002%2fpros.23101&partnerID=40&md5=10835f69427cb4f1655bac5857581ea3},
affiliation={Laboratory of Oncoendocrinology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Genetic Engineering, Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg, Russian Federation; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg, Russian Federation; Department of Genetics and Biotechnology, Saint Petersburg State University, Saint-Petersburg, Russian Federation; Department of Urology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Cell Migration and Invasion, Faculty of Medicine in the Galilee, Bar-Ilan University, Safed, Israel},
abstract={BACKGROUND Prostate cancer is the most common cancer in men. Prostate-specific antigen has, however, insufficient diagnostic specificity. Novel complementary diagnostic approaches are greatly needed. MiRNAs are small regulatory RNAs which play an important role in tumorogenesis and are being investigated as a cancer biomarker. In addition to their intracellular regulatory functions, miRNAs are secreted into the extracellular space and can be found in various body fluids, including urine. The stability of extracellular miRNAs is defined by association with proteins, lipoprotein particles, and membrane vesicles. Among the known forms of miRNA packaging, tumour-derived exosome-enclosed miRNAs is thought to reflect the vital activity of cancer cells. The assessment of the exosomal fraction of urinary miRNA may present a new and highly specific method for prostate cancer diagnostics; however, this is challenged by the absence of reliable and inexpensive methods for isolation of exosomes. METHODS Prostate cancer (PC) cell lines and urine samples collected from 35 PC patients and 35 healthy donors were used in the study. Lectins, phytohemagglutinin, and concanavalin A were used to induce agglutination of exosomes. The efficiency of isolation process was evaluated by AFM and DLS assays. The protein content of isolated exosomes was analysed by western blotting. Exosomal RNA was assayed by automated electrophoresis and expression level of selected miRNAs was evaluated by RT-qPCR. The diagnostic potency of the urinary exosomal miRNA assessment was estimated by the ROC method. RESULTS The formation of multi-vesicular agglutinates in urine can be induced by incubation with lectin at a final concentration of 2 mg/ml. These agglutinates contain urinary exosomes and may be pelleted by centrifugation with a relatively low G-force. The analysis of PC-related miRNA in urinary exosomes revealed significant up-regulation of miR-574-3p, miR-141-5p, and miR-21-5p associated with PC. CONCLUSIONS Lectin-induced aggregation is a low-cost and easily performed method for isolation of exosomes from urine. Isolated exosomes can be further analysed in terms of miRNA content. The miRNA profile of urinary exosomes reflects development of prostate cancer and may present a promising diagnostic tool. Prostate 76:68-79, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.},
author_keywords={diagnostic;  exosomes;  lectin;  miRNA;  prostate cancer},
correspondence_address1={Malek, A.; NN Petrov Institute of Oncology, Leningradskaya 68, Russian Federation; email: anastasia@malek.com},
publisher={John Wiley and Sons Inc.},
issn={02704137},
coden={PRSTD},
pubmed_id={26417675},
language={English},
abbrev_source_title={Prostate},
document_type={Article},
source={Scopus},
}



@ARTICLE{Chernous2015778,
author={Chernous, S.A. and Shvets, M.V. and Filatov, M.V. and Shagimuratov, I.I. and Kalitenkov, N.V.},
title={Studying navigation signal singularities during auroral disturbances},
journal={Russian Journal of Physical Chemistry B},
year={2015},
volume={9},
number={5},
pages={778-784},
doi={10.1134/S1990793115050188},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&doi=10.1134%2fS1990793115050188&partnerID=40&md5=77037f8ce87c89f036cb23c5eabf2ff7},
affiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Western Division, Russian Academy of Sciences, pr. Pobedy 41, Kaliningrad, 236017, Russian Federation; Murmansk State Technical University, ul. Sportivnaya 13, Murmansk, 183010, Russian Federation},
abstract={The experimental studies of the GPS and GLONASS satellite signal singularities, depending on the spatial and time distributions of auroras and geomagnetic disturbances that mark the polar ionosphere current state, are presented. The experimental evidences for positioning errors, related to the spatial–time variations in auroral arcs, are demonstrated. These errors of the navigation systems are observed in an increase in the positioning errors and in a violation of the system integrity. It has been indicated that auroral rayed arcs are indicators of disturbances in the navigation system operation. We tried to explain this fact in the scope of the concept of navigation signal phase fluctuations caused by total electron content inhomogeneities along geomagnetic field lines. © 2015, Pleiades Publishing, Ltd.},
author_keywords={auroral disturbances;  navigation systems;  positioning error sources},
funding_details={Russian Foundation for Basic Research14-07-00512},
funding_details={Russian Foundation for Basic Research4-05-98820r-sever-a},
correspondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Russian Federation},
publisher={Maik Nauka Publishing / Springer SBM},
issn={19907931},
language={English},
abbrev_source_title={Russ. J. Phys. Chem. B},
document_type={Article},
source={Scopus},
}



@ARTICLE{Kovalev2015191,
author={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatova, M.V.},
title={Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations},
journal={Cell and Tissue Biology},
year={2015},
volume={9},
number={3},
pages={191-197},
doi={10.1134/S1990519X15030086},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&doi=10.1134%2fS1990519X15030086&partnerID=40&md5=0c901448296154db7453d31299ecdd32},
affiliation={Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, St. Petersburg State Polytechnical University, St. Petersburg, 194064, Russian Federation; Division of Biosciences, Brunel University, London, United Kingdom},
abstract={The p21/Waf1 protein is one of the main regulators of cell cycle arrest and one of the best-known transcriptional targets of the TP53 protein. Here, we demonstrated that there is activation of expression of the p21/Waf1 gene when the cells were treated with sodium butyrate (NaBu), which is a natural histone deacetylase inhibitor, and investigated whether this phenomenon depends on the presence of a functionally active TP53 protein. For this purpose, we compared the effect of NaBu treatment of human cell lines with different TP53 mutation profiles, including wild-type TP53, single nucleotide substitutions, and the complete absence of the TP53 gene. NaBu activated the TP53 protein via hyperacetylation at the lysine residue K382, without significant changes in the level of protein expression. Western blotting showed that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both TP53 wild-type cells and in cells with single nucleotide substitutions in the central DNA-binding core domain (DBD) of the TP53 protein. At the same time, no p21/Waf1 protein induction was observed in cells with complete deletion of the TP53 gene. However, NaBu was not able to induce p21/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein, but, unexpectedly, it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One possible explanations is that NaBu increases the level of TP53 acetylation and the modified protein is able to establish a new network of protein–protein interactions or trigger conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired. © 2015, Pleiades Publishing, Ltd.},
author_keywords={HDAC inhibitors;  p21/Waf1/Cip1;  RNA interference;  sodium butyrate;  TP53;  TP53 mutations},
correspondence_address1={Filatova, M.V.; Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”Russian Federation},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Morozkina2015411,
author={Morozkina, S.N. and Gluzdikov, I.A. and Drozdov, A.S. and Selivanov, S.I. and Kovalev, R.A. and Filatov, M.V. and Shavva, A.G.},
title={Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens},
journal={Russian Journal of Organic Chemistry},
year={2015},
volume={51},
number={3},
pages={411-416},
doi={10.1134/S1070428015030215},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&doi=10.1134%2fS1070428015030215&partnerID=40&md5=911c9d5ff7da174e600a9815e94f60e9},
affiliation={St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russian Federation; Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center, Orlova Roshcha, Gatchina, Leningrad oblast, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya ul. 29, St. Petersburg, 195251, Russian Federation},
abstract={Sulfamates derived from estrogens of the 8α-series have been synthesized, and their effect on the growth of MCF-7 breast cancer cells has been studied. The best anticancer effect has been found for 7β-methyl-D-homo-6-oxa-8α-estrone sulfamate and 3-methoxy-2,17β-sulfamoyloxy-8α-estra-1,3,5(10)-triene which almost completely inhibit the growth of cancer cells. © 2015 Pleiades Publishing, Ltd.},
correspondence_address1={Shavva, A.G.; St. Petersburg State University, Universitetskii pr. 26, Russian Federation},
publisher={Maik Nauka Publishing / Springer SBM},
issn={10704280},
coden={RJOCE},
language={English},
abbrev_source_title={Russ. J. Org. Chem.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Kovalev2015204,
author={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatov, M.V.},
title={Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53},
journal={Tsitologiia},
year={2015},
volume={57},
number={3},
pages={204-211},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&partnerID=40&md5=bd66a7bb8e7e0ed78b3edf4820c210f3},
abstract={p21/Waf1 protein is one of the main cell cycle arrest regulators and one of the most well-known transcriptional targets of TP53 protein. Here, we demonstrated the activation of expression of the p21/Waf1 gene when the cells were treated to sodium butyrate (NaBu)--one of the natural inhibitors of deacetylase, and investigated whether this phenomenon depends on the presence of functionally active TP53 protein. We compared the effect of the NaBu treatment on the human cell line with different TP53 mutation profile, including: wild-type TP53, single nucleotide substitutions, and the complete absence of TP53 gene. NaBu activated the TP53 protein via hyper acetylation at lysine residue K382, without significant changes in the level of protein expression. Western blotting demonstrated that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both the TP53 wild-type cells and in the cells with single nucleotide substitutions in the domain responsible for the binding of TP53 protein to DNA. At the same time, no the p21/Waf1 protein induction was observed in the cells with complete deletion of the TP53 gene. However, NaBu was not able to induce the p2 1/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that the NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein but unexpectedly it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One of the hypothetical explanations is that NaBu increases the level of TP53 acetylation, and the modified protein is able to establish a new network of protein-protein interactions or trigger some conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired.},
issn={00413771},
pubmed_id={26021170},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Malek2015429,
author={Malek, A.V. and Berstein, L.M. and Filatov, M.V. and Belyaev, A.M.},
title={Exosomal intercellular communications system and its role in the process of metastatic dissemination},
journal={Voprosy Onkologii},
year={2015},
volume={60},
number={4},
pages={429-436},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&partnerID=40&md5=d9a9fcdb72d174dbbcca3823bb3967f2},
affiliation={Ltd. Oncosystem, Russian Federation; N.N. Petrov Research Institute of Oncology, St. Petersburg, Russian Federation; Kurchatov Institute, Gatchina, Russian Federation},
abstract={Metabolism and information between cells is the basis of the existence of any multicellular organism. Malfunction of the intercellular communication play an important and sometimes decisive role in the pathogenesis of the most diseases, including cancer. According to traditional views, functional integration of individual cells in tissues, organs and organ systems is mediated by the efficient work of regulatory systems: nervous, immune, endocrine. Over the past few years the attention of scientists is attracted the ability of cells to «communicate» with the help of nanoscale vesicular formations, or so-called exosomes. There are accumulated data that the cells of the most tissues secrete exosomes into the intercellular environment, after which, by means of stream ofblood or lymph, exosomes transferred to anatomically distant sites where they are accepted by the other cells. It is showed that the content of exosomes are not random and that vesicular transport may be targeted and to play a significant physiological and even «pathophysiological» role. The aim of this review is the analysis and integration of modern scientific data on the role of exosomes in the process of tumor progression and presentation of possible ways and methods of using these data in the practice of oncology.},
author_keywords={Cancer;  Exosomes;  Intercellular contacts;  Metastasis},
publisher={Izdatel'stvo Meditsina},
issn={05073758},
coden={VOONA},
pubmed_id={25552061},
language={Russian},
abbrev_source_title={Vopr. Onkol.},
document_type={Review},
source={Scopus},
}





@ARTICLE{Volnitskiy2014368,
author={Volnitskiy, A.V. and Semenova, E.V. and Shtam, T.A. and Kovalev, R.A. and Filatov, M.V.},
title={Aberrant expression of the sox2 gene in malignant gliomas},
journal={Cell and Tissue Biology},
year={2014},
volume={8},
number={5},
pages={368-373},
doi={10.1134/S1990519X14050101},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&doi=10.1134%2fS1990519X14050101&partnerID=40&md5=761056e34f65f3402cadffe380c42b4e},
affiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},
abstract={Both genetic and epigenetic changes underlie the mechanisms of tumor initiation and progression. In this study, we analyzed sox2 gene expression and its epigenetic changes in primary cultures of malignant gliomas. The sox2 expression was detected in most (74%) gliomas, but not in morphologically normal brain tissue. These facts point to relationships between the sox2 transcription activity and the process of glioma malignant transformation. It was demonstrated that association of different areas of the sox2 gene with important epigenetic markers—posttranslational modifications of H3 histone H3K4ac and H3K9met3—did not correlate with sox2 expression. However, it suggests stochastic regulation of sox2 gene expression in malignant gliomas. © 2014, Pleiades Publishing, Ltd.},
author_keywords={aberrant gene expression;  gliomas;  posttranslational H3 histone modifications;  Sox2},
correspondence_address1={Filatov, M.V.; Konstantinov St. Petersburg Nuclear Physics InstituteRussian Federation},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}









@ARTICLE{Shtam2013,
author={Shtam, T.A. and Kovalev, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Kil, Y.V. and Filatov, M.V.},
title={Exosomes are natural carriers of exogenous siRNA to human cells in vitro},
journal={Cell Communication and Signaling},
year={2013},
volume={11},
number={1},
doi={10.1186/1478-811X-11-88},
art_number={88},
note={cited By 160},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&doi=10.1186%2f1478-811X-11-88&partnerID=40&md5=da53d624b8be3bfd1494f309cb115de5},
affiliation={Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; School of Health Sciences and Social Care, Brunel University, Uxbridge UB8 3PH, United Kingdom; Department of Biophysics, St. Petersburg State Polytechnical University, St.-Petersburg 195251, Russian Federation},
abstract={Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.},
author_keywords={Cancer therapy;  Drug delivery system;  Exosomes;  RAD51;  RNA interference (RNAi)},
correspondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},
issn={1478811X},
pubmed_id={24245560},
language={English},
abbrev_source_title={Cell Commun. Signal.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Yung20131137,
author={Yung, I.A. and Pantina, R.A. and Lebedev, D.V. and Filatov, M.V. and Isaev-Ivanov, V.V.},
title={Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy},
journal={Journal of Surface Investigation},
year={2013},
volume={7},
number={6},
pages={1137-1142},
doi={10.1134/S1027451013060414},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&doi=10.1134%2fS1027451013060414&partnerID=40&md5=90679420c913620df35e1385632cd253},
affiliation={National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation},
abstract={The distribution of replication origins in the nuclei of different cells is studied by confocal microscopy. Based on the obtained images, three-dimensional maps of the positions of the origin centers is constructed and the distribution functions of the pair distances between them are calculated. It is established that the distance distribution function for HeLa and glioma human cells is linear at sizes up to 2 μm, which indicates that the size of the origin system is close to 2. The amplitude of the distance distribution function at small sizes has a power dependence on the nucleus size and is inversely proportional to the nucleus volume to the power of 0.9. Thus, the replication-origin distribution in a nucleus cannot be described by a model with a single Hausdorff dimension in the whole range of sizes. © 2013 Pleiades Publishing, Ltd.},
correspondence_address1={Yung, I. A.; National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation; email: Igor.Yung@rambler.ru},
issn={10274510},
language={English},
abbrev_source_title={J. Surf. Invest.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Bruder20139661,
author={Bruder, J.T. and Chen, P. and Semenova, E. and Thomas, C.A. and Konovalova, S. and Ekberg, G. and Ettyreddy, D. and McVey, D. and Gall, J.G. and King, C.R. and Brough, D.E.},
title={Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors},
journal={Journal of Virology},
year={2013},
volume={87},
number={17},
pages={9661-9671},
doi={10.1128/JVI.00462-13},
note={cited By 8},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&doi=10.1128%2fJVI.00462-13&partnerID=40&md5=d7ebaa5d3acd911b11d1eafd42e0592e},
affiliation={GenVec, Inc., Gaithersburg, MD, United States; International AIDS Vaccine Initiative, AIDS Vaccine Design and Development Laboratory, Brooklyn Army Terminal, Brooklyn, NY, United States},
abstract={We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior exposure to Ad5. However, hexon-modified Ad5 vectors displayed an approximate 10-fold growth defect in complementing cells, making potential vaccine costs unacceptably high. Replacing hypervariable regions (HVRs) 1, 2, 4, and 5 with the equivalent HVRs from Ad43 was sufficient to avoid Ad5 preexisting immunity and retain full vaccine potential. However, the resulting vector displayed the same growth defect as the hexon-modified vector carrying all 9 HVRs from Ad43. The growth defect is likely due to a defect in capsid assembly, since DNA replication and late protein accumulation were normal in these vectors. We determined that the hexon-modified vectors have a 32°C cold-sensitive phenotype and selected revertants that restored vector productivity. Genome sequencing identified a single base change resulting in a threonine-to-methionine amino acid substitution at the position equivalent to residue 342 of the wild-type protein. This mutation has a suppressor phenotype (SP), since cloning it into our Ad5 vector containing all nine hypervariable regions from Ad43, Ad5.H(43m-43), increased yields over the version without the SP mutation. This growth improvement was also shown for an Ad5-based hexon-modified vector that carried the hexon hypervariable regions of Ad48, indicating that the SP mutation may have broad applicability for improving the productivity of different hexon-modified vectors. © 2013, American Society for Microbiology.},
funding_details={National Institutes of Health1R43 AI077309-01},
correspondence_address1={Bruder, J.T.; GenVec, Inc., Gaithersburg, MD, United States; email: joetbruder@gmail.com},
issn={0022538X},
coden={JOVIA},
pubmed_id={23824800},
language={English},
abbrev_source_title={J. Virol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova2013303,
author={Semenova, E.V. and Filatov, M.V.},
title={Genetic and epigenetic markers of gliomas},
journal={Cell and Tissue Biology},
year={2013},
volume={7},
number={4},
pages={303-313},
doi={10.1134/S1990519X13040123},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&doi=10.1134%2fS1990519X13040123&partnerID=40&md5=d829132a07f3116bdb8a07052fe396d0},
affiliation={Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},
abstract={Malignant gliomas are aggressive and highly invasive tumors. Various genetic and epigenetic changes are common for these tumors. Mostly they concern the genes involved in cell-cycle regulation, apoptotic pathways, cell invasion, angiogenesis, and cell metabolism. The role of epigenetic mechanisms in glioma malignant transformation, despite recent progress, is uncertain and remains under intense study. This review describes the mechanisms of epigenetic regulation of gene expression, including posttranslational modifications of histones, DNA methylation in promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used for diagnostics and new therapeutic approaches are also discussed. © 2013 Pleiades Publishing, Ltd.},
author_keywords={epigenetic alterations;  genetic particularities;  gliomas},
correspondence_address1={Semenova, E. V.; Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: semenova_el.spb@mail.ru},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Review},
source={Scopus},
}



@ARTICLE{Babkina2013131,
author={Babkina, L.A. and Garmai, Y.P. and Lebedev, D.V. and Pantina, R.A. and Filatov, M.V. and Isaev-Ivanov, V.V.},
title={Using Zernike moments for analysis of images},
journal={Numerical Analysis and Applications},
year={2013},
volume={6},
number={2},
pages={131-144},
doi={10.1134/S1995423913020055},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&doi=10.1134%2fS1995423913020055&partnerID=40&md5=ae022d86a3cfca1a839a87e852e65b87},
affiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Biophysics Scientific-Educational Structure at the St. Petersburg State Polytechnic University, Konstantinov Petersburg Nuclear Physics Institute, St. Petersburg, Russian Federation},
abstract={A method for analyzing AFM images of the cell nuclei of higher organisms by expanding these images by Zernike moments is proposed. This method allows for expanding the pilot image by Zernike moments whose spatial harmonics are Zernike polynomials. It is shown that the reverse procedure of image reconstruction using Zernike polynomials converges to the experimental image and the expansion amplitude is a quantitative spectral characteristic in comparing the morphological features of different images. It is shown that expansion amplitudes can be used as input vectors for cluster analysis of images by PCA. © 2013 Pleiades Publishing, Ltd.},
author_keywords={atomic force microscopy;  cell nuclei of higher organisms;  image analysis;  PCA;  Zernike moments},
funding_details={Ministry of Education and Science of the Russian Federation2.2.1.1/1166},
}

@ARTICLE{Semenova2013290,
author={Semenova, E.V. and Filatov, M.V.},
title={Genetic and epigenetic markers of gliomas},
journal={Tsitologiya},
year={2013},
volume={55},
number={5},
pages={290-299},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&partnerID=40&md5=9827c4d438c05b1e4f0ec867aae49af4},
affiliation={B. P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},
abstract={Malignant gliomas, aggressive and highly invasive tumors with a great number of genetic and epigenetic alterations in genes involved in the cell cycle regulation, the apoptotic pathways, cell invasion ability, and angiogenesis, are considered to be among the deadliest of human cancers. The role of epigenetic mechanisms in the pathogenesis of malignant transformation despite recent progress is not yet clear elucidated and remains under intensive study. This review describes the mechanisms of epigenetic regulation of gene expression, including post-translational modification of histones, DNA methylation in the promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used in the development of diagnostics and new therapeutic approaches are also discussed.},
author_keywords={Epigenetic alterations;  Genetic particularity;  Gliomas},
publisher={Maik Nauka Publishing / Springer SBM},
issn={00413771},
coden={TSITA},
pubmed_id={24592735},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Kovalev2012800,
author={Kovalev, R.A. and Shtam, T.A. and Ibatullin, F.M. and Bondarev, G.N. and Filatov, M.V.},
title={In vitro potential of histone deacetylase inhibitors for anticancer therapy},
journal={Voprosy Onkologii},
year={2012},
volume={58},
number={6},
pages={800-807},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&partnerID=40&md5=330a4b66718da144b754fc8a404b3fd9},
affiliation={Nuclear Physics Institute, Saint-Petersburg, Russian Federation},
abstract={Research during the past decade has shown that epigenetic events have a key role in carcinogenesis and tumour progression. Histone deacetylase inhibitors (HDACi) comprise structurally diverse compounds that are a group of targeted epigenetic anticancer agents. Here we explored the in vitro efficacy of HDACi such as sodium butyrate (BuNa), valproic acid (VaNa) and several novel HDAC inhibitors for the treatment of cancer. Both BuNa and VaNa inhibited cancer cell proliferation in a time - and dose-dependent fashion. In the present study we demonstrated the significant effect of two novel HDACi, Adipo or BuNHOH, able to induce apoptosis of cancer cells, but not of normal line. Since HDAC inhibitors have been proposed as radio - or chemosensitizers in cancer therapy, we have studied the radiosensitizing effect of sodium butyrate on cancer cells. The combination of BuNa and radiation significantly inhibited tumor cell growth. Besides, combining Cisplatin or Gemzar with HDAC inhibitors results in synergistic antiproliferative activity that could be therapeutically exploited. These results suggest that HDACi acts as an antitumor agent and that combining HDAC inhibitors with radio or - chemotherapeutic strategy may provide a novel chemotherapeutic treatment of cancers insensitive to traditional antitumor agents.},
correspondence_address1={Kovalev, R.A.; Nuclear Physics Institute, Saint-Petersburg, Russian Federation},
issn={05073758},
coden={VOONA},
pubmed_id={23600307},
language={Russian},
abbrev_source_title={Vopr. Onkol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Volnyzky2012545,
author={Volnyzky, A.V. and Vinogradskaya, G.R. and Filatov, M.V.},
title={P73 gene expression in gliomas},
journal={Voprosy Onkologii},
year={2012},
volume={58},
number={4},
pages={545-548},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&partnerID=40&md5=81ac9932d0f3ad831e25dbfd7c3c5d62},
affiliation={B.P.Konstantinov Saint-Petersburg, Institute for Nuclear Physics, Russian Federation},
abstract={We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.},
correspondence_address1={Volnyzky, A.V.; B.P.Konstantinov Saint-Petersburg, Institute for Nuclear PhysicsRussian Federation},
issn={05073758},
coden={VOONA},
pubmed_id={23607213},
language={Russian},
abbrev_source_title={Vopr. Onkol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Ilatovskiy2012298,
author={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},
title={Current insights into chromatin structure organization},
journal={Tsitologiya},
year={2012},
volume={54},
number={4},
pages={298-306},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&partnerID=40&md5=fe62933099f2553f60478e65194b7c41},
affiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},
abstract={This review summarizes current insights into organization of chromatin structure at different levels of DNA compaction. Analysis of available experimental data allowed concluding that only nucleosomal level of structural organization was sufficiently investigated, whereas structure of a 30-nm chromatin fiber remains an open issue. The data on the chromatin structure obtained at the level of the nucleus speak in favor of a biphasic fractal organization of chromatin.},
author_keywords={30-nm fiber;  Chromatin;  DNA;  Fractal;  Nucleosome},
correspondence_address1={Ilatovskiy, A.V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: andrcyi@omrb.pnpi.spb.ru},
issn={00413771},
coden={TSITA},
pubmed_id={22724366},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Review},
source={Scopus},
}



@ARTICLE{Shtam2012430,
author={Shtam, T.A. and Naiyzhny, S.N. and Landa, S.B. and Burdackov, V.S. and Artamonova, T.O. and Filatov, M.V.},
title={Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro},
journal={Tsitologiya},
year={2012},
volume={54},
number={5},
pages={430-438},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&partnerID=40&md5=66c4733440532eaa83659d67c8673764},
affiliation={B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},
abstract={Exosomes are 20-100 nm membrane vesicles of endocytic origin secreted by most cell types in vitro and in vivo. Since exosomes contain both RNA (mRNA and microRNA) and proteins, which can be transferred to another cell, and be functional in that new environment, these vesicles may be involved in the communication between cells. The secretion of exosomes by tumor cells and their implication in the transport and propagation of infectious cargo suggest their participation in pathological situations. Our purpose here is to describe methods for the production, purification, and proteomic characterization of exosomes derived from human cancer cells in vitro. Based on exosomes' unique lipidic composition, we have developed the new approach to increase production of exosomes by cells in vitro. Secondly, we have developed quality control by laser correlation spectroscopy for exosomal assays based on the amount of MHC class I and CD63 molecules on their surface. At last, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used after 2D electrophoresis for the proteomic analysis of exosomes derived from cancer cell lines. This study describes the protein composition of brain tumor cell-derived exosomes in more detail.},
author_keywords={Exosomes;  Laser correlation spectroscopy;  Proteomic analysis},
correspondence_address1={Filatov, M.V.; B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil53@mail.ru},
issn={00413771},
coden={TSITA},
pubmed_id={22827041},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Borghi201219034,
author={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},
title={Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109)},
journal={Proceedings of the National Academy of Sciences of the United States of America},
year={2012},
volume={109},
number={46},
pages={19034},
doi={10.1073/pnas.1217417109},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&doi=10.1073%2fpnas.1217417109&partnerID=40&md5=893faf9b8234bde71083129c7163f3ff},
correspondence_address1={Borghi, N.},
publisher={National Academy of Sciences},
issn={00278424},
coden={PNASA},
language={English},
abbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},
document_type={Erratum},
source={Scopus},
}



@ARTICLE{Soroka2012734,
author={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiian, G.A. and Aplin, K.D. and Gridasov, G.G.},
title={[Absorption of iodofolic acids by the cells of malignant tumors].},
journal={Bioorganicheskaia khimiia},
year={2012},
volume={38},
number={6},
pages={734-744},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&partnerID=40&md5=551a1ed8267ba2c6cffd52472fbc6df1},
abstract={The uptake of 125-iodine labeled 3' iodofolic acid (I*F) and 3' iodo, 5 formyl tetrahydrofolic acid (I*FT) by the cells HeLa, ECV, L-41, human glioma, and rat glioma was studied. Human Embrionic Lung Fibroblasts (HELF) were taken for comparison as healthy cells. It was shown for *IF that its long-term uptake by cells L-41 and ECV is hundreds oftimes higher than those of HELF cells. The short-term uptake phase was studied for *IFT uptake. The dissociation constant was determined for a complex formed by *IFT and an acceptor in the HeLa cells, which is supposed to cause concentrative uptake of *IFT in cells. The dissociation constants of this acceptor complexes with folic acid, 3' iodofolic acid and 3',5'-diiodofolic acid were determined by competition with I*FT. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and rats with a sarcoma grafted on his thigh and glioma grafted into the brain was studied. As was shown there are large differences in the concentration of *IF and *IFT in the tumor and in the healthy tissue, *IF concentration in thigh muscle of healthy being 5 times lower than those in tumor grafted to the thigh, and *IFT concentration in healthy brain being 10 times lower than in brain tumor.},
correspondence_address1={Soroka, N.V.},
issn={01323423},
pubmed_id={23547477},
language={Russian},
abbrev_source_title={Bioorg. Khim.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Soroka2012652,
author={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiyan, G.A. and Aplin, K.D. and Gridasov, G.G.},
title={Absorption of iodofolic acids by the cells of malignant tumors},
journal={Russian Journal of Bioorganic Chemistry},
year={2012},
volume={38},
number={6},
pages={652-661},
doi={10.1134/S1068162012060131},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&doi=10.1134%2fS1068162012060131&partnerID=40&md5=f27639cceea9ebc8bc5cd19271332c20},
affiliation={Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; Khlopin Radium Institute, Russian Federation},
abstract={The uptake of our synthesized 125-iodine labeled 3'-iodofolic acid ( *IF) and 3'-iodo-5-formyltetrahyrofolic acid ( *IFT) by the HeLa, EVC, L-41, human glioma, and rat glioma cells has been studied. The Human Embryonic Lung Fibroblasts (HELFs) were used as healthy control cells for comparison. It was shown for the quantity of *IF absorbed at its long-term uptake phase by the L-41 and ECV cells is hundreds of times higher than that of the HELF cells. The short-term *IFT uptake phase by the cells was studied. For the HeLa cells, the dissociation constant was determined for the *IFT complex with an acceptor, which is supposed to be responsible for *IFT accumulation in the cells. Using competitive sorption approach, the dissociation constants of folic, 3'-iodofolic, and 3',5'-diiodofolic acid complexes with this acceptor were assessed. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and also in rats with sarcoma grafted on their thigh and glioma grafted into the brain was studied. A considerable differences were shown in the concentrations of *IF and *IFT in the tumor and in the healthy tissue, i.e., *IF in the healthy thigh muscle is five times lower than in the tumor grafted to the thigh, and *IFT concentration is 10 times lower in the healthy brain compared to the brain tumor. © Pleiades Publishing, Ltd., 2012.},
author_keywords={Absorption;  Folates;  Iodine derivatives;  Isotope 125I;  Malignant cells},
correspondence_address1={Filatov, M.V.; Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; email: fil53ster@gmail.ru},
issn={10681620},
coden={RJBCE},
language={English},
abbrev_source_title={Russ. J. Bioorg. Chem.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Sun2012,
author={Sun, X.-L. and Yang, T. and Guan, J.-P. and Ma, Y. and Jiang, J.-Y. and Cao, R. and Burlyaeva, M. and Vishnyakova, M. and Semenova, E. and Bulyntsev, S. and Zong, X.-X.},
title={Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae)},
journal={American Journal of Botany},
year={2012},
volume={99},
number={10},
pages={e379-e390},
doi={10.3732/ajb.1100346},
note={cited By 15},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&doi=10.3732%2fajb.1100346&partnerID=40&md5=16a3d02c0039ad7f415ea0468134678f},
affiliation={Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Biological Sciences, China Agricultural University, Beijing 100193, China; Department of Leguminous Crops Genetic Resources, N. I. Vavilov Research Institute of Plant Industry, St. Petersburg 190000, Russian Federation},
abstract={• Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. • Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. • Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea. © 2012 Botanical Society of America.},
author_keywords={Expressed sequence tag;  Fabaceae;  Grasspea;  Lathyrus sativus;  Microsatellite;  Primer},
correspondence_address1={Zong, X.-X.; Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; email: zongxx@mail.caas.net.cn},
issn={00029122},
coden={AJBOA},
pubmed_id={23028003},
language={English},
abbrev_source_title={Am. J. Bot.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Borghi201212568,
author={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},
title={E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch},
journal={Proceedings of the National Academy of Sciences of the United States of America},
year={2012},
volume={109},
number={31},
pages={12568-12573},
doi={10.1073/pnas.1204390109},
note={cited By 284},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&doi=10.1073%2fpnas.1204390109&partnerID=40&md5=27a25f91d046dadaaa32776ddecb5cfa},
affiliation={Department of Biology, Stanford University, Stanford, CA 94305, United States; Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, United States; Department of Cell Biology, Centre National de la Recherche Scientifique, Université Paris-Diderot, Paris 75013, France},
abstract={Classical cadherins are transmembrane proteins at the core of intercellular adhesion complexes in cohesive metazoan tissues. The extracellular domain of classical cadherins forms intercellular bonds with cadherins on neighboring cells, whereas the cytoplasmic domain recruits catenins, which in turn associate with additional cytoskeleton binding and regulatory proteins. Cadherin/catenin complexes are hypothesized to play a role in the transduction of mechanical forces that shape cells and tissues during development, regeneration, and disease. Whether mechanical forces are transduced directly through cadherins is unknown. To address this question, we used a Förster resonance energy transfer (FRET)-based molecular tension sensor to test the origin and magnitude of tensile forces transmitted through the cytoplasmic domain of E-cadherin in epithelial cells. We show that the actomyosin cytoskeleton exerts pN-tensile force on E-cadherin, and that this tension requires the catenin-binding domain of E-cadherin and αE-catenin. Surprisingly, the actomyosin cytoskeleton constitutively exerts tension on E-cadherin at the plasma membrane regardless of whether or not E-cadherin is recruited to cell-cell contacts, although tension is further increased at cell-cell contacts when adhering cells are stretched. Our findings thus point to a constitutive role of E-cadherin in transducing mechanical forces between the actomyosin cytoskeleton and the plasmamembrane, not only at cell-cell junctions but throughout the cell surface.},
author_keywords={Mechanobiology;  Mechanosensor;  Mechanotransduction;  Morphogenesis;  Signal transduction},
correspondence_address1={Dunn, A.R.; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; email: alex.dunn@stanford.edu},
publisher={National Academy of Sciences},
issn={00278424},
coden={PNASA},
pubmed_id={22802638},
language={English},
abbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shtam2012317,
author={Shtam, T.A. and Naryzhny, S.N. and Landa, S.B. and Burdakov, V.S. and Artamonova, T.O. and Filatov, M.V.},
title={Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells},
journal={Cell and Tissue Biology},
year={2012},
volume={6},
number={4},
pages={317-325},
doi={10.1134/S1990519X12040116},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&doi=10.1134%2fS1990519X12040116&partnerID=40&md5=621ea006f96d0bd38461fec3a60fde94},
affiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},
abstract={Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that quantitative and qualitative characterization of these microparticles produced by different tissues in normal and pathological states can give valuable diagnostic and prognostic information and be a biomarker of different diseases, including oncological ones. Elaboration of the purification of exosomes and their proteome analysis was the aim of the present work. An original approach to enhancing exosome production in cultured transformed human cells was developed. The data obtained allowed us to detect exosomes in cultural conditioned samples and control the quality of produced exosomes at all stages of their purification. Electrophoretic analysis of proteins obtained from exosomes of different origins shows differences in protein profiles. Proteins from exosomes of glioblastoma cell lines were separated by two-dimensional electrophoresis. Protein profiles were further analyzed by densitometry and mass spectrometry, which allowed more than 30 proteins, including specific tumor markers, to be identified. © 2012 Pleiades Publishing, Ltd.},
author_keywords={exosomes;  laser correlation spectroscopy;  proteome analysis},
correspondence_address1={Filatov, M. V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil_53@mail.ru},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Sim2012854,
author={Sim, J.Y. and Borghi, N. and Ribeiro, A. and Sorokina, M. and Shcherbakova, O. and Ramallo, D. and Dunn, A. and Nelson, W.J. and Pruitt, B.L.},
title={Uniaxial cell stretcher enables high resolution live cell imaging},
journal={Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)},
year={2012},
pages={854-857},
doi={10.1109/MEMSYS.2012.6170320},
art_number={6170320},
note={cited By 2; Conference of 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference Date: 29 January 2012 Through 2 February 2012;  Conference Code:89445},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&doi=10.1109%2fMEMSYS.2012.6170320&partnerID=40&md5=10bc083a2824dc630c10ffed9ff11ea6},
affiliation={Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; Biology, Stanford University, CA, United States; Chemical Engineering, Stanford University, CA, United States},
abstract={Imaging adherent cells cultured on commercially available stretchable substrates presents challenges for high-magnification objectives. Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson's contraction with stretching, and issues of optical transparency or non-matching refractive indices. Beyond the advantages of visualizing biological structures in detail, we seek to implement specialized high-resolution fluorescence microscopy techniques such as Förster Resonance Energy Transfer (FRET) microscopy while stretching. Enabling FRET imaging of stretched cells provides a powerful tool for modern cell biology and mechanobiology [1]. FRET techniques require high magnification as well as a stable focal plane for imaging. Here, we address this requirement for stretchable substrates with a microfabricated cell strain device suitable for live cell imaging while allowing high-resolution FRET microscopy of cells. We adapt a uniaxial cell stretching concept previously demonstrated by Huh [2] for high magnification imaging by using thin bottom channels and membranes supported above an inverted oil immersion objective. This work presents a major advance for research in cell mechanobiology as it enables direct mechanical actuation combined with imaging of FRET probes engineered to report strained proteins in live cells. © 2012 IEEE.},
correspondence_address1={Sim, J.Y.; Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; email: simba85@stanford.edu},
sponsors={Robotics and Automation Society; IEEE; Region Nord-Pas de Calais},
address={Paris},
issn={10846999},
isbn={9781467303248},
coden={PMEME},
language={English},
abbrev_source_title={Proc. IEEE Int. Conf. Micro Electro Mech. Syst. MEMS},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Bruder2012,
author={Bruder, J.T. and Semenova, E. and Chen, P. and Limbach, K. and Patterson, N.B. and Stefaniak, M.E. and Konovalova, S. and Thomas, C. and Hamilton, M. and King, C.R. and Richie, T.L. and Doolan, D.L.},
title={Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses},
journal={PLoS ONE},
year={2012},
volume={7},
number={4},
doi={10.1371/journal.pone.0033920},
art_number={e33920},
note={cited By 24},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&doi=10.1371%2fjournal.pone.0033920&partnerID=40&md5=615d0f3eb9de5faa02e836da1c904c89},
affiliation={Research, GenVec, Inc., Gaithersburg, MD, United States; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States; Queensland Institute of Medical Research, Brisbane, Australia; University of California San Diego, La Jolla, CA, United States; MedImmune, Inc., Gaithersburg, MD, United States; International Aids Vaccine Initiative, New York, NY, United States},
abstract={The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.},
correspondence_address1={Bruder, J. T.; Research, GenVec, Inc., Gaithersburg, MD, United States; email: jbruder@genvec.com},
issn={19326203},
pubmed_id={22496772},
language={English},
abbrev_source_title={PLoS ONE},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Ilatovskiy2012,
author={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},
title={SANS spectra of the fractal supernucleosomal chromatin structure models},
journal={Journal of Physics: Conference Series},
year={2012},
volume={351},
number={1},
doi={10.1088/1742-6596/351/1/012007},
art_number={012007},
note={cited By 7; Conference of 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich's Birth ; Conference Date: 27 May 2011 Through 30 May 2011;  Conference Code:89603},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&doi=10.1088%2f1742-6596%2f351%2f1%2f012007&partnerID=40&md5=4cb28476bafca2cc34da1a137dfb05ea},
affiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},
abstract={The eukaryotic genome consists of chromatin - a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions - 3 and 2. © Published under licence by IOP Publishing Ltd.},
correspondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},
sponsors={Comenius University in Bratislava; Horia Hulubei Natl. Inst. Phys. Nucl. Eng. (IFIN HH); Institute of Macromolecular Chemistry AS CR; Jt. Inst. Nucl. Res., Frank Lab. Neutron Phys.},
publisher={Institute of Physics Publishing},
address={Dubna},
issn={17426588},
language={English},
abbrev_source_title={J. Phys. Conf. Ser.},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Semenova2012139,
author={Semenova, E.V. and Shadrina, M.I. and Slominsky, P.A. and Ivanova-Smolenskaya, I.A. and Bagyeva, G. and Illarioshkin, S.N. and Limborska, S.A.},
title={Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson's disease},
journal={Movement Disorders},
year={2012},
volume={27},
number={1},
pages={139-143},
doi={10.1002/mds.23901},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&doi=10.1002%2fmds.23901&partnerID=40&md5=6dc7221fa668d60863daa976f09cf055},
affiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation; Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russian Federation},
abstract={Background:: Deletions and duplications of single exons or exon groups account for a large proportion of the PARK2 gene mutations described in juvenile autosomal recessive Parkinson's disease (PD). Methods:: We analyzed rearrangements in exons 1 to 12 of the PARK2 gene in Russian sporadic patients with early-onset PD (EOPD) and late-onset PD (LOPD). Results:: The frequency of EOPD and LOPD patients carrying these mutations was 12.4% and 3.8%, respectively. The most frequent rearrangements were detected in exons 3 and 4. The odds ratio for EOPD in individuals carrying PARK2 exon deletions and duplications was 13.95 (95% confidence interval [CI], 1.846-105.46; P = .0022). In addition, we found a correlation between exon rearrangements in PARK2 and the age at onset of PD, presence of dystonia, and symmetrical course of the disease. Conclusions:: Exon rearrangements in the PARK2 gene play a significant role in the pathogenesis of sporadic PD in Russian patients. © 2011 Movement Disorder Society.},
author_keywords={Deletion;  Duplication;  PARK2 exon rearrangements;  Parkinson's disease},
correspondence_address1={Illarioshkin, S.N.Volokolamskoe Shosse 80, Moscow 125367, Russian Federation; email: snillario@gmail.com},
issn={08853185},
coden={MOVDE},
pubmed_id={21915905},
language={English},
abbrev_source_title={Mov. Disord.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Ilatovskiy2011,
author={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Grigoriev, M. and Petukhov, M.G. and Isaev-Ivanov, V.V.},
title={Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale},
journal={Journal of Applied Physics},
year={2011},
volume={110},
number={10},
doi={10.1063/1.3661987},
art_number={102217},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&doi=10.1063%2f1.3661987&partnerID=40&md5=8a4868aeeffdd6637dac55d1e4fa84a9},
affiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnical University, St. Petersburg, Russian Federation; Laboratoire Biologie Moléculaire Eukaryote, CNRS-Université Paul-Sabathier, Toulouse, France},
abstract={Eukaryotic genome is a highly compacted nucleoprotein complex organized in a hierarchical structure based on nucleosomes. Detailed organization of this structure remains unknown. In the present work we developed algorithms for geometry modeling of the supernucleosomal chromatin structure and for computing distance distribution functions and small-angle neutron scattering (SANS) spectra of the genome-scale (∼106 nucleosomes) chromatin structure at residue resolution. Our physical nucleosome model was based on the mononucleosome crystal structure. A nucleosome was assumed to be rigid within a local coordinate system. Interface parameters between nucleosomes can be set for each nucleosome independently. Pair distance distributions were computed with Monte Carlo simulation. SANS spectra were calculated with Fourier transformation of weighted distance distribution; the concentration of heavy water in solvent and probability of H/D exchange were taken into account. Two main modes of supernucleosomal structure generation were used. In a free generation mode all interface parameters were chosen randomly, whereas nucleosome self-intersections were not allowed. The second generation mode (generation in volume) enabled spherical or cubical wall restrictions. It was shown that calculated SANS spectra for a number of our models were in general agreement with available experimental data. © 2011 American Institute of Physics.},
correspondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},
issn={00218979},
coden={JAPIA},
language={English},
abbrev_source_title={J Appl Phys},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Semenova201116,
author={Semenova, E.V. and Filatov, M.V.},
title={Study of chromatin decondensation factors in human spermatozoids by flow cytometry},
journal={Russian Journal of Developmental Biology},
year={2011},
volume={42},
number={1},
pages={16-24},
doi={10.1134/S1062360411010097},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&doi=10.1134%2fS1062360411010097&partnerID=40&md5=925eb3cf7929f0e3f073ac255b316768},
affiliation={Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation},
abstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm. © 2011 Pleiades Publishing, Ltd.},
author_keywords={endogenous nuclear proteases;  fertilization;  flow cytometry;  human spermatozoids;  protease inhibitors},
correspondence_address1={Filatov, M. V.; Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},
issn={10623604},
language={English},
abbrev_source_title={Russ. J. Dev. Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova201120,
author={Semenova, E.V. and Filatov, M.V.},
title={[Study of chromatin decondensation factors in human spermatozoids by flow cytometry].},
journal={Ontogenez},
year={2011},
volume={42},
number={1},
pages={20-29},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&partnerID=40&md5=e4b4997f53d5d4069bc4d51f710afbb4},
abstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm.},
correspondence_address1={Semenova, E.V.},
issn={04751450},
pubmed_id={21442899},
language={Russian},
abbrev_source_title={Ontogenez},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov201035,
author={Filatov, M.V. and Landa, S.B. and Pantina, R.A. and Garmai, Yu.P.},
title={Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo},
journal={Klinichescheskaya Laboratornaya Diagnostika},
year={2010},
number={12},
pages={35-43},
note={cited By 8},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&partnerID=40&md5=c3b287531eda58217f247310540fc220},
abstract={Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection ofexosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked.},
author_keywords={Dynamic light scattering technique;  Exosomes;  Fibroblasts;  Lymphocytes},
correspondence_address1={Filatov, M. V.email: filatov@omrb.pnpi.spb.ru},
issn={08692084},
pubmed_id={21395053},
language={Russian},
abbrev_source_title={Klin. Lab. Diagn.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Varfolomeeva2010485,
author={Varfolomeeva, E.Y. and Ivanov, E.I. and Drobchenko, E.A. and Semenova, E.V. and Filatov, M.V.},
title={Detection of inflammatory processes during various diseases by the method of flow cytofluorometry},
journal={Bulletin of Experimental Biology and Medicine},
year={2010},
volume={149},
number={4},
pages={485-489},
doi={10.1007/s10517-010-0976-2},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&doi=10.1007%2fs10517-010-0976-2&partnerID=40&md5=283fbb036945227dc1e8fc31f3e078a3},
affiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},
abstract={Oxidative (respiratory) burst is an important manifestation of inflammation. Precise quantitative assessment of this reaction by flow cytometry made it possible to record and evaluate the severity of the inflammatory processes in a wide spectrum of diseases including diphtheria, hepatitis, pneumonia, bronchial asthma, arthritis, vasculitis, postoperative complications, tuberculosis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and myocardial infarction. This approach can be employed as a highly sensitive method of detection of inflammatory reactions and monitoring of their course in various pathological processes. © 2010 Springer Science+Business Media, Inc.},
author_keywords={flow cytometry;  inflammation;  respiratory burst},
correspondence_address1={Varfolomeeva, E. Y.; St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation; email: e_varf@mail.ru},
issn={00074888},
coden={BEXBA},
pubmed_id={21234449},
language={English},
abbrev_source_title={Bull. Exp. Biol. Med.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Alexeyev2010364,
author={Alexeyev, V.Yu. and Kaboev, O.K. and Scherbakova, O.G. and Semenova, E.V. and Filatov, M.V.},
title={Immunological similarity of diphtheria toxin and EGF receptor},
journal={Tsitologiya},
year={2010},
volume={52},
number={5},
pages={364-370},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&partnerID=40&md5=8a292ba5a5e9c827cff8d9e690c24c38},
affiliation={Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States; Molecular and Radiation Biophysics Division of St. Petersburg, Nuclear Physics Institute, RAS, St. Petersburg, Gatchina, Russian Federation; Department of Molecular and Cellular Physiology, Stanford University, Stanford, United States},
abstract={Cardiomyopathy and neuropathy are the two commonly observed complications in diphtheria patients and in, some instances, individuals vaccinated against diphtheria. The nature of these complications remains not well understood. It was suggested that autoimmunity may play a role in the development of these afflictions. Based on functional similarities between diphtheria toxin (DT) and epidermal growth factor receptor (EGFR), which both can bind to the heparin-binding EGF-like growth factor (HB-EGF) precursors, we suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized by antibodies raised against EGFR and by serum antibodies from diphtheria patients. Moreover serum of diphtheria patients competitively inhibits binding of anti-EGFR antibodies to the receptor. The truncated diphtheria toxin without B-subunit could be detected by serum antibodies of diphtheria patients, but not by anti-EGFR antibodies. Collectively, these studies demonstrate cross-reactivity of antibodies raised against B-subunit of DT and extracellular domain of EGFR and suggest that clinically observed post-diphtheria complications may result from autoimmune inhibition of EGFR function and possible destruction of receptor-positive tissues.},
author_keywords={Cross-immunoreactivity;  Diphtheria toxin;  Epidermal growth factor receptor;  Post-diphtheria complications},
correspondence_address1={Alexeyev, V. Yu.; Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States},
issn={00413771},
coden={TSITA},
pubmed_id={20586270},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Isaev-Ivanov20101063,
author={Isaev-Ivanov, V.V. and Lebedev, D.V. and Lauter, H. and Pantina, R.A. and Kuklin, A.I. and Islamov, A.K. and Filatov, M.V.},
title={Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering},
journal={Physics of the Solid State},
year={2010},
volume={52},
number={5},
pages={1063-1073},
doi={10.1134/S1063783410050379},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&doi=10.1134%2fS1063783410050379&partnerID=40&md5=b7266c4aa86093bcd4dc258952790714},
affiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; Research and Educational Center Biophysics of the St. Petersburg State Polytechnical University, Konstantinov Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Institut Laue-Langevin, BP 156, Grenoble 38042, France; Joint Institute for Nuclear Research, ul. Joliot-Curie 6, Dubna, Moscow oblast 141980, Russian Federation},
abstract={The nucleosome structure in native nuclei of normal (chicken erythrocyte and rat leukocyte nuclei) and anomalously proliferating (the human cervical adenocarcinoma cell line HeLa and the Chinese hamster fibroblast cell line A238) cells has been investigated using small-angle neutron scattering. The experimental results obtained allow one to make the inference that the parameters of the nucleosome structure for the chicken erythrocyte and rat leukocyte nuclei (on average over the nucleus) are close to the universally accepted values and that the distance distribution function is bimodal. The bimodality of the distance distribution function reflects a narrow distribution of distances between nucleosomes (on average over the nucleus) at the fibril level of the chromatin organization. The histone core of the nucleosome structure in the nuclei of the HeLa and A238 cells (on average over the nucleus) is considerably less compact than that in the chicken erythrocyte and rat leukocyte nuclei, and the distance distribution function does not exhibit indications of the bimodality. © 2010 Pleiades Publishing, Ltd.},
correspondence_address1={Filatov, M. V.; Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},
issn={10637834},
language={English},
abbrev_source_title={Phys. Solid State},
document_type={Article},
source={Scopus},
}



@ARTICLE{Angelotti2010560,
author={Angelotti, T. and Daunt, D. and Shcherbakova, O.G. and Kobilka, B. and Hurt, C.M.},
title={Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal},
journal={Traffic},
year={2010},
volume={11},
number={4},
pages={560-578},
doi={10.1111/j.1600-0854.2010.01033.x},
note={cited By 29},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&doi=10.1111%2fj.1600-0854.2010.01033.x&partnerID=40&md5=58b7e08a45bab6703d592151df3ebfcd},
affiliation={Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 259, Campus Drive, Stanford, CA 94035, United States},
abstract={Plasma membrane (PM) expression of G-protein coupled receptors (GPCRs) is required for activation by extracellular ligands; however, mechanisms that regulate PM expression of GPCRs are poorly understood. For some GPCRs, such as alpha2c-adrenergic receptors (α2c-ARs), heterologous expression in non-native cells results in limited PM expression and extensive endoplasmic reticulum (ER) retention. Recently, ER export/retentions signals have been proposed to regulate cellular trafficking of several GPCRs. By utilizing a chimeric α2a/α2c-AR strategy, we identified an evolutionary conserved hydrophobic sequence (ALAAALAAAAA) in the extracellular amino terminal region that is responsible in part for α2c-AR subtype-specific trafficking. To our knowledge, this is the first luminal ER retention signal reported for a GPCR. Removal or disruption of the ER retention signal dramatically increased PM expression and decreased ER retention. Conversely, transplantation of this hydrophobic sequence into α2a-ARs reduced their PM expression and increased ER retention. This evolutionary conserved hydrophobic trafficking signal within α2c-ARs serves as a regulator of GPCR trafficking. © 2010 John Wiley &amp; Sons A/S.},
author_keywords={ER export;  ER retention;  GPCR trafficking;  Protein sorting;  Retention signal},
correspondence_address1={Angelotti, T.; Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; email: timangel@stanford.edu},
issn={13989219},
coden={TRAFF},
pubmed_id={20059747},
language={English},
abbrev_source_title={Traffic},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova2009138,
author={Semenova, E.V.},
title={Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1},
journal={Ėksperimental'naia i klinicheskaia gastroėnterologiia = Experimental & clinical gastroenterology},
year={2009},
number={6},
pages={138-142},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&partnerID=40&md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9},
abstract={The purpose of the research has been the study of possibilities for the pharmacological correction of the morphological changes in liver and lipid metabolism disorders in experimental type 1 diabetes. Simvastatin, new compound from the group of antioxidants--3-hydroxipyridine derivate under the code HP-5, and also the combination of simvastatin and HP-5 have been used for the pharmacological correction in this research. It was revealed that the using of HP-5 and the combination of simvastatin and HP-5 attenuate the order of expression of diabetic hepatopathia as the result of the histological study and hinder from the development of dislipidemia in rates with type 1 diabetes which is more expressed in comparison with the monotherapy by simvastatin. These changes also are followed with the diminishing of the free radical lipid oxidation activity by the specific antioxidant effect of the investigated 3-hydroxipyridine derivate.},
correspondence_address1={Semenova, E.V.},
issn={16828658},
pubmed_id={20201297},
language={Russian},
abbrev_source_title={Eksp Klin Gastroenterol},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Galich2009,
author={Galich, N.E. and Filatov, M.V.},
title={Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment},
journal={Proceedings of SPIE - The International Society for Optical Engineering},
year={2009},
volume={7377},
doi={10.1117/12.836452},
art_number={73770C},
note={cited By 2; Conference of 12th International Workshop on Nanodesign Technology and Computer Simulations ; Conference Date: 23 June 2008 Through 27 June 2008;  Conference Code:77311},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&doi=10.1117%2f12.836452&partnerID=40&md5=f3819171ad8b578019e72414bc891193},
affiliation={Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; Department of Cell Biology, B.P.Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 183300, Russian Federation},
abstract={Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status. © 2009 SPIE.},
author_keywords={Attractor;  Bifurcation;  Blood;  Cell;  Cytometry;  Distribution;  DNA;  Immunofluorescence;  Neutrophils;  Oxidants},
correspondence_address1={Galich, N. E.; Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; email: n.galich@mail.ru},
sponsors={Motorola Company; Research and Production Corporation Integral; Scanwest Company; Silvaco Data Systems},
address={Minsk},
issn={0277786X},
isbn={9780819476531},
coden={PSISD},
language={English},
abbrev_source_title={Proc SPIE Int Soc Opt Eng},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Semenova2009573,
author={Semenova, E.V. and Shadrina, M.I. and Slominskiǐ, P.A. and Illarioshkin, S.N. and Bagyeva, G.K. and Karabanov, A.V. and Ivanova-Smolenskaia, I.A. and Limborskaia, S.A.},
title={Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease},
journal={Genetika},
year={2009},
volume={45},
number={4},
pages={573-576},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&partnerID=40&md5=f39cf36c04fe528f017aa5d1c25c72f9},
abstract={Fifty-two patients that had ParkinsonTs disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4--6 of alpha-synuclein gene using real-time PCR with Taq-Man probes. No mutations involving the examined exons dosage were revealed in alpha-synuclein gene. Thus, mutations modifying copy number of alpha-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of ParkinsonTs disease in patients from Russia.},
correspondence_address1={Semenova, E.V.},
issn={00166758},
pubmed_id={19507712},
language={Russian},
abbrev_source_title={Genetika},
document_type={Article},
source={Scopus},
}





@ARTICLE{Shtam2008958,
author={Shtam, T.A. and Varfolomeeva, E.Y. and Semenova, E.V. and Filatov, M.V.},
title={Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival},
journal={Tsitologiya},
year={2008},
volume={50},
number={11},
pages={958-963},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&partnerID=40&md5=5313418d75141a24ef0926ff2a58d2ff},
affiliation={Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},
abstract={The RAD 51 protein, an eukaryotic homologue of Escherichia coli Rec A, plays a central role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of Rad5l gene by specific small interfering (si) RNA induces cell death of the most part of investigated cancer cell lines and normal fibroblasts. Disruption of the Rad51 gene in these cells results in S or(and) G 2 cell cycle arrest leading to apoptosis. But some human cancer cell lines demonstrate abolishment of premitotic checkpoint and are not sensitive to siRNA silencing of RAD51 recombinase. Recent experiments show that normal functioning of the recombination repair system is essential for maintenance of genome stability, proliferation of vertebrate cells and, finally, for prevention of dramatic cell death.},
author_keywords={Cell cycle;  DNA reparation;  Homologous recombination;  Rad51},
correspondence_address1={Shtam, T. A.; Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},
issn={00413771},
coden={TSITA},
pubmed_id={19140342},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}





@ARTICLE{Landa200837,
author={Landa, S.B. and Filatov, M.V. and Arutyunyan, A.V. and Varfolomeyeva, Ye.V.},
title={Study of plasma megamolecular complexation by laser correlation spectroscopy},
journal={Klinichescheskaya Laboratornaya Diagnostika},
year={2008},
number={4},
pages={37-41},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&partnerID=40&md5=a30b0b5ac1d900684a623822c2fcc6b0},
issn={08692084},
pubmed_id={18619204},
language={Russian},
abbrev_source_title={Klin. Lab. Diagn.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Lebedev2008110,
author={Lebedev, D.V. and Filatov, M.V. and Kuklin, A.I. and Islamov, A.Kh. and Stellbrink, J. and Pantina, R.A. and Denisov, Yu.Yu. and Toperverg, B.P. and Isaev-Ivanov, V.V.},
title={Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization},
journal={Crystallography Reports},
year={2008},
volume={53},
number={1},
pages={110-115},
doi={10.1134/S1063774508010136},
note={cited By 18},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&doi=10.1134%2fS1063774508010136&partnerID=40&md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf},
affiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningrad oblast 188300, Russian Federation; Biofizika Research and Education Center, St. Petersburg State Polytechnic University, Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Joint Institute of Nuclear Research, ul. Zholio-Kyuri 6, Dubna, Moscow Oblast 141980, Russian Federation; Research Centre Jülich, Jülich 52425, Germany},
abstract={The chromatin organization in chicken erythrocyte nuclei was studied by small-angle neutron scattering in the scattering-vector range from 1.5 × 10-1 to 10-4 Å-1 with the use of the contrast-variation technique. This scattering-vector range corresponds to linear dimensions from 4 nm to 6 μm and covers the whole hierarchy of chromatin structures, from the nucleosomal structure to the entire nucleus. The results of the present study allowed the following conclusions to be drawn: (1) both the chromatin-protein structure and the structure of the nucleic acid component in chicken erythrocyte nuclei have mass-fractal properties, (2) the structure of the protein component of chromatin exhibits a fractal behavior on scales extending over two orders of magnitude, from the nucleosomal size to the size of an entire nucleus, and (3) the structure of the nucleic acid component of chromatin in chicken erythrocyte nuclei is likewise of a fractal nature and has two levels of organization or two phases with the crossover point at about 300-400 nm. © 2008 Pleiades Publishing, Inc.},
funding_details={Российский Фонд Фундаментальных Исследований (РФФИ)02-04-49259-a},
funding_details={Ministry of Education and Science of the Russian FederationRNP 2.2.1.1.4663},
funding_details={Ministry of Education and Science of the Russian Federation40.012.1.1.1149},
}

@BOOK{Naryzhny2007453,
author={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},
title={Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it},
journal={From Genome to Proteome: Advances in the Practice & Application of Proteomics},
year={2007},
pages={453-458},
doi={10.1002/9783527613489.ch59},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&doi=10.1002%2f9783527613489.ch59&partnerID=40&md5=41038eec12b571fb984fee210e4729d5},
affiliation={Petersburg Nuclear Physics Institute of Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation},
abstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA. © 2000 WILEY-VCH Verlag GmbH. All rights reserved.},
author_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional Polyacrylamide gel electrophoresis},
correspondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute of Russian Academy of SciencesRussian Federation; email: naryzhny@omrb.pnpi.spb.ru},
publisher={Wiley Blackwell},
isbn={9783527613489; 9783527301546},
language={English},
abbrev_source_title={From Genome to Proteome: Adv. in the Pract. & Appl. of Proteomics},
document_type={Book Chapter},
source={Scopus},
}



@ARTICLE{Shtam2007194,
author={Shtam, T.A. and Pantina, R.A. and Varfolomeyeva, Ye.Yu. and Filatov, M.V.},
title={Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression},
journal={Voprosy Onkologii},
year={2007},
volume={53},
number={2},
pages={194-199},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&partnerID=40&md5=dd7854f4ddd307cb94f5c9ebb3362452},
affiliation={B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},
abstract={The study was concerned with identification of predictive value of p53 expression on sensitivity to tamoxifen in breast cancer management. Estrogen receptor-positive cell line MCF-7 was used to establish p53 expression influence on the rate of cell proliferation after tamoxifen. The investigation demonstrated the absence of that effect when p53 was silenced.},
correspondence_address1={Shtam, T. A.; B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},
issn={05073758},
coden={VOONA},
language={Russian},
abbrev_source_title={Vopr. Onkol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova2007,
author={Semenova, E.V. and Inchina, V.I.},
title={Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis},
journal={Ėksperimental'nai&x0361a i klinicheskai&x0361a gastroėnterologii&x0361a = Experimental & clinical gastroenterology},
year={2007},
number={6},
pages={122-125, 131},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&partnerID=40&md5=e4f22788b05bbb2755dfab001ca19b6e},
abstract={The authors have studied hepatoprotective actions of new derivatives of 3-hydroxipyridine on an experimental model of toxic hepatitis (140 white mice). Mexidol and berlithion are choosed as the preparations of comparison. The method of light microscopy is used for the exploration of morphological changes. The cytolytic contents activity, catalase activity and the level of MDA have been determined in blood serum. The antitoxic effect is valued by the survival of the animals. It is found that all examined bonds is corrected morphological changes in toxic hepatitis and increased the index of animals survival, which is more expressed than the preparations of comparison.},
correspondence_address1={Semenova, E.V.},
issn={16828658},
pubmed_id={18416106},
language={Russian},
abbrev_source_title={Eksp Klin Gastroenterol},
document_type={Article},
source={Scopus},
}



@CONFERENCE{Galich2007,
author={Galich, N.E. and Filatov, M.V.},
title={Laser fluorescence fluctuation excesses in molecular immunology experiments},
journal={Proceedings of SPIE - The International Society for Optical Engineering},
year={2007},
volume={6597},
doi={10.1117/12.726756},
art_number={65970L},
note={cited By 5; Conference of Nanodesign, Technology, and Computer Simulations ; Conference Date: 19 June 2006 Through 25 June 2006;  Conference Code:70585},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&doi=10.1117%2f12.726756&partnerID=40&md5=1e708809433caac2e8f0ce3082166aba},
affiliation={St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation; B.P.Konstantinov Petersburg Nuclear Physics Institute, St.Petersburg, 183300, Russian Federation},
abstract={A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.},
author_keywords={Cytofluorescence histogram;  Excesses;  Inflammation;  Respiratory burst;  Statistic analysis},
correspondence_address1={Galich, N.E.; St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation},
sponsors={SPIE Poland Chapter; SPIE Russia Chapter},
address={Olsztyn},
issn={0277786X},
isbn={0819467332; 9780819467331},
coden={PSISD},
language={English},
abbrev_source_title={Proc SPIE Int Soc Opt Eng},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Carey2007942,
author={Carey, G.B. and Semenova, E. and Qi, X. and Keegan, A.D.},
title={IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway},
journal={Cell Research},
year={2007},
volume={17},
number={11},
pages={942-955},
doi={10.1038/sj.cr.2007.90},
note={cited By 24},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&doi=10.1038%2fsj.cr.2007.90&partnerID=40&md5=9a31d0fb63047d78fac19588b492e312},
affiliation={Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland of School of Medicine, Baltimore, MD, United States},
abstract={Interleukin-4 (IL-4) promotes lymphocyte survival and protects primary lymphomas from apoptosis. Previous studies reported differential requirements for the signal transducer and activator of transcription 6 (STAT6) and IRS2/phosphatidylinositol 3 kinase (PI-3K) signaling pathways in mediating the IL-4-induced protection from Fas-mediated apoptosis. In this study, we characterized IL-4-activated signals that suppress anti-IgM-mediated apoptosis and growth arrest of CH31, a model B-cell lymphoma line. In CH31, anti-IgM treatment leads to the loss of mitochondrial membrane potential, phospho-Akt, phospho-CDK2, and c-myc protein. These losses are followed by massive induction of p27Kip1 protein expression, cell cycle arrest, and apoptosis. Strikingly, IL-4 treatment prevented or reversed these changes. Furthermore, IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD. IL-4 treatment also induced expression of BclxL, a STAT6-dependent gene. Pharmacologic inhibitors and dominant inhibitory forms of PI-3K and Akt abrogated the anti-apoptotic function of IL-4. These results suggest that the IL-4 receptor activates several signaling pathways, with the Akt pathway playing a major role in suppression of the apoptotic program activated by anti-IgM. © 2007 IBCB, SIBS, CAS. All rights reserved.},
author_keywords={Akt;  Apoptosis;  Cell cycle;  IL-4;  PI-3 kinase},
correspondence_address1={Keegan, A.D.; Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; email: akeegan@som.umaryland.edu},
issn={10010602},
pubmed_id={17968425},
language={English},
abbrev_source_title={Cell Res.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shadrina2007,
author={Shadrina, M.I. and Semenova, E.V. and Slominsky, P.A. and Bagyeva, G.H. and Illarioshkin, S.N. and Ivanova-Smolenskaia, I.I. and Limborska, S.A.},
title={Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage},
journal={BMC Medical Genetics},
year={2007},
volume={8},
doi={10.1186/1471-2350-8-6},
art_number={6},
note={cited By 33},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&doi=10.1186%2f1471-2350-8-6&partnerID=40&md5=bb4117dd790d75c9fea41d1ab154724f},
affiliation={Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; Department of Neurogenetics, Institute of Neurology, 80 Volokolamskoye Highway, 123367 Moscow, Russian Federation},
abstract={Background: One of the causes of Parkinson's disease is mutations in the PARK2 gene. Deletions and duplications of single exons or exon groups account for a large proportion of the gene mutations. Direct detection of these mutations can be used for the diagnosis of Parkinson's disease. Methods: To detect these mutations, we developed an effective technique based on the real-time TaqMan PCR system, which allows us to evaluate the copynumbers of the PARK2 gene exons by comparing the intensity of the amplification signals from some exon of this gene with that of the β-globin gene (the internal control). Results: We analyzed rearrangements in exons 1-12 of the PARK2 gene in 64 patients from Russia with early-onset Parkinson's disease. The frequency of these mutations in our patients was 14%. Conclusion: We have developed a simple, accurate, and reproducible method applicable to the rapid detection of exon rearrangements in the PARK2 gene. It is suitable for the analysis of large patient groups, and it may become the basis for a diagnostic test. © 2007 Shadrina et al; licensee BioMed Central Ltd.},
correspondence_address1={Semenova, E.V.; Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; email: semenova-helen@mail.ru},
issn={14712350},
coden={BMGMA},
pubmed_id={17324265},
language={English},
abbrev_source_title={BMC Med. Genet.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shcherbakova2007521,
author={Shcherbakova, O.G. and Hurt, C.M. and Xiang, Y. and Dell'Acqua, M.L. and Zhang, Q. and Tsien, R.W. and Kobilka, B.K.},
title={Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes},
journal={Journal of Cell Biology},
year={2007},
volume={176},
number={4},
pages={521-533},
doi={10.1083/jcb.200604167},
note={cited By 64},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&doi=10.1083%2fjcb.200604167&partnerID=40&md5=b033b7ab6098f89f08e082e6f4d61463},
affiliation={Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; Department of Pharmacology, University of Colorado, Denver and Health Sciences Center, Aurora, CO 80045, United States; Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States},
abstract={The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo. © The Rockefeller University Press.},
correspondence_address1={Kobilka, B.K.; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; email: kobilka@stanford.edu},
issn={00219525},
coden={JCLBA},
pubmed_id={17296797},
language={English},
abbrev_source_title={J. Cell Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shadrina200631,
author={Shadrina, M.I. and Slominsky, P.A. and Zherebtsova, A.L. and Levitsky, G.N. and Levitskaya, N.I. and Alekhin, A.V. and Semenova, E.V. and Serdyuk, A.V. and Skvortsova, V.L. and Limborska, S.A.},
title={Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population},
journal={Balkan Journal of Medical Genetics},
year={2006},
volume={9},
number={1-2},
pages={31-41},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&partnerID=40&md5=3032bf178ce2c5b63c1c8996952a79f9},
affiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; Department of Fundamental and Clinical Neurology, Russian State Medical University, Moscow, Russian Federation},
abstract={Motor neuron disease (MND) results in selective degeneration of motor neurons of the cerebral cortex, brain stem and spinal cord. It is a complex disease in the pathogenesis of which many genetic systems may be involved. A significant etiological factor of MND may be oxygen free radicals which damage neuronal cells when present in high concentrations. In detoxification processes, free radicals may be formed that become transformed into non toxic products. The major participants of these processes are the cytochromes P-450 CYP2E1, CYP2D6, the glutathione-S-transferases GSTM1, GSTT1, GSTP1, and the N-acetyltransferase NAT2. To investigate their role in the development of MND, we have studied polymorphisms in these genes in 75 Russian patients with MND and 105 controls. We have established a statistical distinction in frequencies of CYP2E1*1D, and GSTM1 (0/0) homozygotes between the patient and the control samples. Whereas the analysis of the CYP2D6, GSTT1, GSTP1 and NAT2 gene polymorphisms has revealed no differences, the GSTP1*A/GSTP1*A genotype was associated with classical upper and lower motor neuron involvement. The GSTP1*B allele was associated predominantly with lower and upper motor neuron involvement. We propose that the genes of phases I and II of the detoxification system, CYP2E1, GSTP1, and GSTM1, participate in the development of sporadic MND in patients in Russia.},
author_keywords={Detoxification processes;  Gene polymorphisms;  Motor neuron disease (MND)},
correspondence_address1={Shadrina, M.I.; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; email: shadrina@img.ras.ru},
issn={13110160},
coden={BJMGF},
language={English},
abbrev_source_title={Balk. J. Med. Genet.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Brum2006397,
author={Brum, P.C. and Hurt, C.M. and Shcherbakova, O.G. and Kobilka, B. and Angelotti, T.},
title={Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons},
journal={Neuropharmacology},
year={2006},
volume={51},
number={3},
pages={397-413},
doi={10.1016/j.neuropharm.2006.03.032},
note={cited By 28},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&doi=10.1016%2fj.neuropharm.2006.03.032&partnerID=40&md5=14ace5ce3f4045c0266da4c7f86c81d5},
affiliation={Laboratório de Fisiologia do Exercício, Escola de Educação Física e Esporte, Universidade de Sao Paulo, Av. Prof. Mello Moraes 65, 05508-900 Sao Paulo, SP, Brazil; Department of Molecular and Cellular Physiology, Stanford University Medical School, 157 Beckman Center, 279 Campus Drive, Stanford, CA 94305, United States; Department of Anesthesia, Stanford University Medical School, Stanford, CA 94305, United States},
abstract={Previous research suggested that α2A and α2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed α2A and α2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, α2A and α2CARs could be readily detected in SGN from wild-type mice. By Day 8, α2AARs were targeted to cell body, as well as axonal and dendritic sites, whereas α2CARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, α2CARs had relocated to somatodendritic and axonal sites and, unlike α2AARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for α2ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous α2A and α2CAR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of α2CARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between α2A and α2CARs in SGN. © 2006 Elsevier Ltd. All rights reserved.},
author_keywords={α2 adrenergic receptor;  Dexmedetomidine;  SV2;  Sympathetic ganglion neuron;  Trafficking},
funding_details={GM07365},
funding_details={98/14765-7},
}

@ARTICLE{Konevega2006669,
author={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Peshekhonov, A.V. and Katunin, V.I.},
title={The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli},
journal={Molekuliarnaia biologiia.},
year={2006},
volume={40},
number={4},
pages={669-683},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&partnerID=40&md5=7eb6533d775986f49cbe70542290ec2d},
abstract={A modified nucleotide on the 3'-side of the anticodon loop of tRNA is one of the most important structure element regulating codon-anticodone interaction on the ribosome owing to the stacking interaction with the stack of codon-anticodon bases. The presence and identity (pyrimidine, purine or modified purine) of this nucleotide has an essential influence on the energy of the stacking interaction on A- and P-sites of the ribosome. There is a significant influence of the 37-modification by itself on the P-site, whereas there is no such one on the A-site of the ribosome. Comparison of binding enthalpies of tRNA interactions on the P- or A-site of the ribosome with the binding enthalpies of the complex of two tRNAs with the complementary anticodones suggests that the ribosome by itself significantly endows in the thermodynamics of codon-anticodon complex formation. It happens by additional ribosomal interactions with the molecule of tRNA or indirectly by the stabilization of codon-anticodon conformation. In addition to the stacking, tRNA binding in the A and P sites is futher stabilized by the interactions involving some magnesium ions. The number of them involved in those interactions strongly depends on the nucleotide identity in the 37-position of tRNA anticodon loop.},
correspondence_address1={Konevega, A.L.},
issn={00268984},
pubmed_id={16913226},
language={Russian},
abbrev_source_title={Mol. Biol. (Mosk.)},
document_type={Review},
source={Scopus},
}





@ARTICLE{Zherebtsova2006104,
author={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitskiǐ, G.N. and Alekhin, A.V. and Slominskiǐ, P.A. and Skvortsova, V.I. and Limborskaia, S.A.},
title={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population},
journal={Genetika.},
year={2006},
volume={42},
number={1},
pages={104-109},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&partnerID=40&md5=9d531826cec7c322a8080b0ec3f07a61},
abstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND.},
correspondence_address1={Zherebtsova, A.L.},
issn={00166758},
pubmed_id={16523673},
language={Russian},
abbrev_source_title={Genetika},
document_type={Article},
source={Scopus},
}



@ARTICLE{Zherebtsova200689,
author={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitsky, G.N. and Alekhin, A.V. and Slominsky, P.A. and Skvortsova, V.I. and Limborska, S.A.},
title={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population},
journal={Russian Journal of Genetics},
year={2006},
volume={42},
number={1},
pages={89-93},
doi={10.1134/S1022795406010133},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&doi=10.1134%2fS1022795406010133&partnerID=40&md5=480e9bf567ea472b2cd6ccec20a9d4c5},
affiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russian Federation; Russian State Medical University, Moscow, 129327, Russian Federation},
abstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND. © Pleiades Publishing, Inc., 2006.},
funding_details={Российский Фонд Фундаментальных Исследований (РФФИ)04-04-49 124a, 02-04-49 076},
}



@ARTICLE{Ivanov2004740,
author={Ivanov, A.V. and Bakhlanova, I.V. and Pantina, R.A. and Filatov, M.V.},
title={Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil'noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.]},
journal={Tsitologiia},
year={2004},
volume={46},
number={8},
pages={740-747},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&partnerID=40&md5=448c2f76d3cf6675cc6b1eff278aefab},
abstract={The phenomenon of loosing exogenic DNA from the mammalian somatic cell genome is under investigation. It is found that foreign DNA incorporated into cell genome as a result of transfection by electrophoretion may be lost with the frequency from 1/100 up to 1/100 000 per cell division during cultivation. This effect is not dependent of the nature of cell line and vector DNA. It is actual for different cell lines: A23, human fibroblasts AG 11395, murine embryonic line F9, and for different plasmid vectors: p16, p.39, pATR4 and pcDNA3.1-Higr (WRN). Integration of pDNA into genome and the following loosing of this DNA is registered by selection markers G418 and hygromycin B resistance and gancyclovir sensibility. The presence of foreign DNA in the genome was controlled by PCR. It is found that true foreign DNA deletion from the genome takes place rather than gene expression changes. For closely linked plasmid genes deletion of both genes at once as well as loosing any one gene separately is shown. Thus, the phenomenon of selective deletion of exogenic DNA from genome has been demonstrated for different mammalian cells.},
correspondence_address1={Ivanov, A.V.},
issn={00413771},
pubmed_id={15598021},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Konevega200490,
author={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Semenkov, Y.P. and Wintermeyer, W. and Rodnina, M.V. and Katunin, V.I.},
title={Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions},
journal={RNA},
year={2004},
volume={10},
number={1},
pages={90-101},
doi={10.1261/rna.5142404},
note={cited By 73},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&doi=10.1261%2frna.5142404&partnerID=40&md5=c0ac9c400bbb3e2d3a57147153225962},
affiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany; Institute of Physical Biochemistry, University of Witten/Herdecke, 58448 Witten, Germany},
abstract={The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNAPhe binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNAPhe with hypermodified G (wybutine), or of unmodified peptidyl-tRNAPhe transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg 2+ ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg2+ -dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon-anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg2+ binding, and interactions with 16S rRNA.},
author_keywords={Ribosome decoding;  Translation;  TRNA conformational change;  TRNA modifications;  TRNA selection},
correspondence_address1={Katunin, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; email: katunin@omrb.pnpi.spb.ru},
issn={13558382},
coden={RNARF},
pubmed_id={14681588},
language={English},
abbrev_source_title={RNA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Soboleva2003121,
author={Soboleva, N.G. and Makhno, V.I. and Konevega, A.L. and Semenkov, Yu.P. and Katunin, V.I.},
title={The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome},
journal={Molekulyarnaya Biologiya},
year={2003},
volume={37},
number={1},
pages={121-127},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&partnerID=40&md5=5c8ad0bf4218457eab4cbe1a75ed3345},
affiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},
abstract={To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA K +Y Phe and Phe-tRNA K -Y Phe ) with the A site of complex [70S · poly(U) · deacylated tRNA Phe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA K +Y Phe showed, removal of the Y base decreased the association constant of Phe-tRNA K -Y Phe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA Phe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA K -Y Phe but not for Phe-tRNA K +Y Phe . Thus, the modified nucleotide 3′ of the Phe-tRNA Phe anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.},
correspondence_address1={Soboleva, N.G.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},
issn={00268984},
coden={MOBIB},
pubmed_id={12624954},
language={Russian},
abbrev_source_title={Mol. Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Marzi2003958,
author={Marzi, S. and Knight, W. and Brandi, L. and Caserta, E. and Soboleva, N. and Hill, W.E. and Gualerzi, C.O. and Lodmell, J.S.},
title={Ribosomal localization of translation initiation factor IF2},
journal={RNA},
year={2003},
volume={9},
number={8},
pages={958-969},
doi={10.1261/rna.2116303},
note={cited By 43},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&doi=10.1261%2frna.2116303&partnerID=40&md5=b40491c4a5016c07d44c5c9308bbc767},
affiliation={Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino (MC), Italy; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States},
abstract={Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit's factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.},
author_keywords={BABE;  fMet-tRNA;  IF2;  Phenanthroline;  Ribosome topography;  Tethered nuclease;  Translation initiation},
correspondence_address1={Lodmell, J.S.; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States; email: lodmell@selway.umt.edu},
issn={13558382},
coden={RNARF},
pubmed_id={12869707},
language={English},
abbrev_source_title={RNA},
document_type={Article},
source={Scopus},
}





@ARTICLE{Semenova2002475,
author={Semenova, E.V. and Drobchenko, E.A. and Filatov, M.V.},
title={The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility},
journal={Tsitologiya},
year={2002},
volume={44},
number={5},
pages={475-476},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&partnerID=40&md5=6cd83eb630a9b3ded1792a7091a98ebf},
affiliation={St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation},
abstract={On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.},
correspondence_address1={Semenova, E.V.; St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation; email: filatov@omrb.pnpi.spb.ru},
issn={00413771},
coden={TSITA},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Safronova2002346,
author={Safronova, I.Yu. and Semenova, E.V.},
title={Maleic acid utilization by mixed cultures of microorganisms},
journal={Applied Biochemistry and Microbiology},
year={2002},
volume={38},
number={4},
pages={346-348},
doi={10.1023/A:1016283005773},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&doi=10.1023%2fA%3a1016283005773&partnerID=40&md5=688457cdf35a3f7a28492b01e8879a50},
affiliation={Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation},
abstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp, xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and B. subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions of water.},
correspondence_address1={Safronova, I.Yu.; Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},
issn={00036838},
language={English},
abbrev_source_title={Appl. Biochem. Microbiol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Levina2002574,
author={Levina, V.V. and Drobchenko, E.A. and Sukhareva, E.B. and Shabalina, E.V. and Filatov, M.V.},
title={A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing»},
journal={Tsitologiya},
year={2002},
volume={44},
number={6},
pages={574-575},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&partnerID=40&md5=6a3d91e3788a2afd0731b1467fd0e5a9},
abstract={The process of active dissociation of noncovalently bound agents from DNA or «DNA clearing» in the living cells was described elsewhere. The vital fluorescent bisbenzimidazole dye Hoechst 33342 (4342), which binds tightly but not covalently to DNA in the minor groove, was used for studying interactions of agents noncovalently binding with DNA. The «DNA clearing» is an energy-dependent process, which is suppressed by topoisomerase-II inhibitors and DNA breaks. It has been shown that the rodent fibroblast cell line AA8HoeR-7 is selected for resistance to H342, and characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. This cell line obtained cross-resistance to other DNA damaging drugs: mitomycin C, etoposide and ethidium bromide. That proves that AA8HoeR-7 is cell line with a new mechanism of multidrug resistance.},
issn={00413771},
coden={TSITA},
pubmed_id={12236101},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Safronova2002403,
author={Safronova, I.Yu. and Semenova, E.V.},
title={Maleic Acid Utilization by Mixed Cultures of Microorganisms},
journal={Prikladnaya Biokhimiya i Mikrobiologiya},
year={2002},
volume={38},
number={4},
pages={403-404},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&partnerID=40&md5=fad79a8ef19fa76e2130fd1bdce1e62c},
affiliation={Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation},
abstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.},
correspondence_address1={Safronova, I.Yu.; Moscow State University, Biological Faculty, Vorob'evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={05551099},
pubmed_id={12325296},
language={Russian},
abbrev_source_title={Prikl. Biokhim. Mikrobiol.},
document_type={Article},
source={Scopus},
}





@ARTICLE{Safronova2001374,
author={Safronova, I.Yu. and Semenova, E.V.},
title={Optimum conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260},
journal={Applied Biochemistry and Microbiology},
year={2001},
volume={37},
number={4},
pages={374-375},
doi={10.1023/A:1010245903543},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&doi=10.1023%2fA%3a1010245903543&partnerID=40&md5=a99a82099320d937b20e7b2a4432d13c},
affiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},
abstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed 98% of the maleic acid (initial concentration of 5.0 g/l medium) under periodic conditions for 48 h. Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans transformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h-1. © 2001 MAIK "Nauka/Interperiodica".},
correspondence_address1={Safronova, I.Yu.; Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},
publisher={Kluwer Academic/Plenum Publishers},
issn={00036838},
language={English},
abbrev_source_title={Appl. Biochem. Microbiol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Safronova2001436,
author={Safronova, I.I. and Semenova, E.V.},
title={Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.]},
journal={Prikladnaia biokhimiia i mikrobiologiia},
year={2001},
volume={37},
number={4},
pages={436-438},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&partnerID=40&md5=9ce3e167f5fe2d61369252ced3a4fd9f},
affiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},
abstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.},
correspondence_address1={Safronova, I.I.},
issn={05551099},
pubmed_id={11530667},
language={Russian},
abbrev_source_title={Prikl. Biokhim. Mikrobiol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Blizniukov200149,
author={Blizniukov, O.P. and Kozmin, L.D. and Oliushin, V.E. and Ostreiko, O.V. and Filatov, M.V.},
title={Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral'noi nervnoi sistemy mogut byt' dostupny dlia ataki spetsificheskimi antitelami.]},
journal={Voprosy onkologii},
year={2001},
volume={47},
number={1},
pages={49-51},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&partnerID=40&md5=9183e57df4f31e5ae6a0b50434f95a09},
affiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation},
abstract={The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.},
correspondence_address1={Blizniukov, O.P.},
issn={05073758},
pubmed_id={11317535},
language={Russian},
abbrev_source_title={Vopr Onkol},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shcherbakova2000829,
author={Shcherbakova, O.G. and Lantsov, V.A. and Filatov, M.V.},
title={Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting},
journal={Doklady Akademii Nauk},
year={2000},
volume={371},
number={6},
pages={829-833},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&partnerID=40&md5=2af0b62eb2bdcdb6f93bb91fdd52b885},
issn={08695652},
coden={DAKNE},
language={Russian},
abbrev_source_title={Dokl. Akad. Nauk},
document_type={Article},
source={Scopus},
}



@ARTICLE{Aleshin200075,
author={Aleshin, V.V. and Semenova, E.V. and Tarakanov, B.V. and Livshits, V.A.},
title={A family öf shuttle vectors 'for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon},
journal={Mikrobiologiya},
year={2000},
volume={69},
number={1},
pages={75-80},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&partnerID=40&md5=fa59c80a48a0c518643554b7cbd2df52},
affiliation={All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},
abstract={A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in grampositive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc,) was created. The vectors are based on the cryptic plasmid pLFOll uom'Lactoimcillus fermentum VKM 1311 belonging to a family.of the o-type pE194-like plasmkls. The vectors can replicite in .gram-positive bacteria and Escherichia coll, "They, are stable in many gram-positive bacteria, "have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that'contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPot can' be mobilized in a great number' of bacteria using a' helper pltlmid from E, coll but not from1 gram-positive bacteria,.},
author_keywords={Gram-positive bacteria;  Lactic acid bacteria;  Shuttle vectors;  Ss-dna plasmids},
correspondence_address1={Aleshin, V.V.; All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},
issn={00263656},
coden={MIKBA},
pubmed_id={10808493},
language={Russian},
abbrev_source_title={Mikrobiologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Golikov200042,
author={Golikov, P.P. and Davydov, B.V. and Marchenko, V.V. and Nikolaeva, N.I. and Golikov, A.P. and Riabinin, V.A. and Semenova, E.V. and Polumiskov, V.I.},
title={Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel'nyi stress pri hypertonicheskikh krizakh.]},
journal={Klinicheskaya Meditsina},
year={2000},
volume={78},
number={7},
pages={42-45},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&partnerID=40&md5=c8abc6251cfd7f665b4becca652d030a},
abstract={Urapidil effects on oxidant stress were studied in 36 patients with hypertension stage I-III running with crises. Acute hypertensive crisis was managed by intravenous injections of urapidil (20-50 mg) for 5 min. After that for a week they received urapidil monotherapy followed by combined treatment for another week (quinapril--25 mg/day or ramipril--5-10 mg/day in combination with hypothiaside--25 mg/day; lokren--20 mg/day or atenolol--50-100 mg/day and diltiazem--180-360 mg/day) to stabilize arterial pressure finally. Oxidant stress was estimated by lipid peroxidation (LPO) and state of antioxidant system (AOS). Catabolism and anabolism were evaluated by blood levels of hydrocortisone and insulin. LPO, AOS, the blood hormones were measured before management of hypertensive crisis, immediately after it and 1, 3, 7 and 14 days after. Before the crisis treatment LPO was found high. Urapidil administration reduced levels of hydrocortisone, dienic conjugates, lipid peroxidation, oxidant stress rate (OSR). Later, LPO and OSR slowly increased. It is concluded on validity of the antioxidants use in hypertensive patients with crises.},
correspondence_address1={Golikov, P.P.},
issn={00232149},
pubmed_id={10979642},
language={Russian},
abbrev_source_title={Klin Med (Mosk)},
document_type={Article},
source={Scopus},
}



@ARTICLE{Levina2000451,
author={Levina, V. and Varfolomeeva, E. and Sukhareva, E. and Shabalina, E. and Drobchenko, E. and Filatov, M.},
title={A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents},
journal={ATLA Alternatives to Laboratory Animals},
year={2000},
volume={28},
number={3},
pages={451-456},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&partnerID=40&md5=70cc6ef737ebc7edab2a5f3f4dcc9536},
affiliation={Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},
abstract={The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step- by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-1-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33342 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.},
author_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Mitomycin C;  Multidrug resistance},
correspondence_address1={Levina, V.; Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},
issn={02611929},
coden={AALAD},
language={English},
abbrev_source_title={ATLA Altern. Lab. Anim.},
document_type={Conference Paper},
source={Scopus},
}





@ARTICLE{Shcherbakova20001,
author={Shcherbakova, O.G. and Filatov, M.V.},
title={Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells},
journal={Biochimica et Biophysica Acta - Molecular Cell Research},
year={2000},
volume={1495},
number={1},
pages={1-3},
doi={10.1016/S0167-4889(99)00151-2},
note={cited By 10},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&doi=10.1016%2fS0167-4889%2899%2900151-2&partnerID=40&md5=da5b2e1f4286e2c7e96d04eac53c4b64},
affiliation={Molec. and Radiat. Biophys. Division, Petersburg Nucl. Phys. Inst., 188350, Gatchina, Russian Federation},
abstract={In order to study the involvement of DNA topoisomerase I (top1) in recombination, we examined the effect of the anti-neoplastic drug camptothecin, which selectively poisons top1 by trapping top1-cleavable complexes on integration of exogenic vector into the genome of mammalian cells. We transfected mouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a plasmid carrying a selectable neo gene treated with camptothecin, and determined the frequency of neo+ (G418(R)) colonies. We found that treatment with camptothecin for as short a time as 4 h after electroporation resulted in a 4- to 33-fold stimulation of plasmid integration into the recipient genome via non-homologous recombination. These results imply that top1-cleavable complexes trapped by camptothecin could be potentially recombinogenic structures and could stimulate non-homologous recombination in vivo, promoting the integration of transfected plasmids into mammalian genome. Copyright (C) 2000 Elsevier Science B.V.},
author_keywords={Camptothecin;  Mammalian somatic cell;  Non-homologous recombination;  Topoisomerase 1;  Transfected DNA},
correspondence_address1={Shcherbakova, O.G.; Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, 188350 Gatchina, Russian Federation; email: olia@omrb.pnpi.spb.ru},
issn={01674889},
coden={BAMRD},
pubmed_id={10634926},
language={English},
abbrev_source_title={Biochim. Biophys. Acta Mol. Cell Res.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Scherbakova200069,
author={Scherbakova, O.G. and Lanzov, V.A. and Filatov, M.V.},
title={Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting.},
journal={Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry section / translated from Russian},
year={2000},
volume={371},
number={1-6},
pages={69-72},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&partnerID=40&md5=6d2b5ab3cc3a34ddf31edb4d0a59e434},
affiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},
correspondence_address1={Scherbakova, O.G.},
issn={00124958},
pubmed_id={10853454},
language={English},
abbrev_source_title={Dokl. Biochem.},
document_type={Article},
source={Scopus},
}







@ARTICLE{Varfolomeeva1999402,
author={Varfolomeeva, E.Yu. and Levina, V.V. and Tret'yakov, A.N. and Drobchenko, E.A. and Filatov, M.V.},
title={Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells},
journal={Doklady Akademii Nauk},
year={1999},
volume={364},
number={3},
pages={402-404},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&partnerID=40&md5=b75439e5584ecc0bb4557fa9b5830fdf},
issn={08695652},
coden={DAKNE},
pubmed_id={10188092},
language={English; Russian},
abbrev_source_title={Dokl. Akad. Nauk},
document_type={Article},
source={Scopus},
}



@ARTICLE{Kaboev1999400,
author={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret'yakov, A.N. and Shcherbakova, O.G.},
title={Hot start of the polymerase chain reaction using DNA helicases},
journal={Bioorganicheskaya Khimiya},
year={1999},
volume={25},
number={5},
pages={400},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&partnerID=40&md5=f0d90567c33a31e593c4e7a9fbbcaedf},
affiliation={Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},
abstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.},
author_keywords={DNA helicase;  Hot start;  PCR},
correspondence_address1={Kaboev, O.K.; Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.spb.ru},
issn={01323423},
language={Russian},
abbrev_source_title={Bioorganicheskaya Khim.},
document_type={Article},
source={Scopus},
}





@ARTICLE{Filatov1999825,
author={Filatov, M.V. and Semenova, E.V. and Vorob'eva, O.A. and Leont'eva, O.A. and Drobchenko, E.A.},
title={Relationship between abnormal sperm chromatin packing and IVF results},
journal={Molecular Human Reproduction},
year={1999},
volume={5},
number={9},
pages={825-830},
doi={10.1093/molehr/5.9.825},
note={cited By 62},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&doi=10.1093%2fmolehr%2f5.9.825&partnerID=40&md5=749736b469111d208fd9a86f6f59e531},
affiliation={Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},
abstract={This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross- linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.},
author_keywords={Chromatin packing;  Ethidium bromide;  Fertility disorder;  Flow cytometry;  Human spermatozoa},
correspondence_address1={Filatov, M.V.; Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},
issn={13609947},
coden={MHREF},
pubmed_id={10460220},
language={English},
abbrev_source_title={Mol. Hum. Reprod.},
document_type={Article},
source={Scopus},
}





@ARTICLE{Naryzhny19991033,
author={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},
title={Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?},
journal={Electrophoresis},
year={1999},
volume={20},
number={4-5},
pages={1033-1038},
doi={10.1002/(SICI)1522-2683(19990101)20:4/5<1033::AID-ELPS1033>3.0.CO;2-3},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&partnerID=40&md5=3f6b63ef15d5a83f4e04cddb3b81eaeb},
affiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, 188350, Russian Federation},
abstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA.},
author_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional polyacrylamide gel electrophoresis},
correspondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad District 188350, Russian Federation; email: naryzhny@omrb.pnpi.spb.ru},
issn={01730835},
coden={ELCTD},
pubmed_id={10344282},
language={English},
abbrev_source_title={Electrophoresis},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Kaboev1999350,
author={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret'yakov, A.N. and Shcherbakova, O.G.},
title={Hot start of the polymerase chain reaction using DNA helicases},
journal={Russian Journal of Bioorganic Chemistry},
year={1999},
volume={25},
number={5},
pages={350-351},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&partnerID=40&md5=72d82556ef27e324b46c8a4c6b27a4b9},
affiliation={St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},
abstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture. © 1999 MAEe cyrillic signK "Hayκa/Interperiodica".},
author_keywords={DNA helicase;  Hot start;  PCR},
correspondence_address1={Kaboev, O.K.; St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.sbr.ru},
issn={10681620},
language={English},
abbrev_source_title={Russ. J. Bioorg. Chem.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Shcherbakova1999951,
author={Shcherbakova, O.G. and Smirnova, T.V. and Filatov, M.V.},
title={Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells},
journal={Tsitologiya},
year={1999},
volume={41},
number={11},
pages={951},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&partnerID=40&md5=ff82ecf873c4549e7c93f8fae7ce5775},
abstract={A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system.},
author_keywords={Gene targeting;  hprt;  Illegitimate recombination;  Poly(ADP-ribose) polymerase;  Topoisomerases},
publisher={Maik Nauka-Interperiodica Publishing},
issn={00413771},
coden={TSITA},
pubmed_id={10643051},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Ivanov19998,
author={Ivanov, E.},
title={Vitamin E: blocking the beginnings of atherosclerosis?},
journal={Molecular medicine today},
year={1999},
volume={5},
number={1},
pages={8},
doi={10.1016/S1357-4310(98)01402-6},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&doi=10.1016%2fS1357-4310%2898%2901402-6&partnerID=40&md5=038c62d4e6a2ed7c1c6f416237e01f51},
correspondence_address1={Ivanov, E.email: eivanov@aol.com},
issn={13574310},
pubmed_id={10088123},
language={English},
abbrev_source_title={Mol Med Today},
document_type={Short Survey},
source={Scopus},
}



@ARTICLE{Tarasov199999,
author={Tarasov, V.A. and Filatov, M.V. and Kisliakova, T.V. and Noskov, F.S. and Koloskov, A.V. and Stavrovietski, V.V. and Onikienko, S.B. and Kletchikov, V.Z. and Lvov, I.V. and Varfolomeeva, E.Yu. and Blizniukov, O.P. and Levina, V.V. and Kiselevski, M.V.},
title={Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer},
journal={Hybridoma},
year={1999},
volume={18},
number={1},
pages={99-102},
doi={10.1089/hyb.1999.18.99},
note={cited By 5},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&doi=10.1089%2fhyb.1999.18.99&partnerID=40&md5=2b2d61bdf666822de71b5967cb78bb5c},
affiliation={Department of Thorax Surgery, Med. Acad. of Postgraduated Educ., 196247, St. Petersburg, Russian Federation; City Hospital No. 26, 196247, St. Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, St. Petersburg, 188350, Russian Federation; Petersburg Nuclear Physics Institute, 188350, Gatchina, St. Petersburg, Russian Federation},
abstract={Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilie de Calmette-Guerin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.},
correspondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, 188350 Gatchina, St. Petersburg, Russian Federation},
publisher={Mary Ann Liebert Inc.},
issn={0272457X},
coden={HYBRD},
pubmed_id={10211796},
language={English},
abbrev_source_title={Hybridoma},
document_type={Conference Paper},
source={Scopus},
}



@ARTICLE{Levina1998706,
author={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},
title={"DNA clearing" from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance},
journal={Biologicheskie Membrany},
year={1998},
volume={15},
number={6},
pages={706-707},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&partnerID=40&md5=a98a72c5e0367996d29f6a7ca63e9233},
affiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},
correspondence_address1={Levina, V.V.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},
issn={02334755},
language={Russian},
abbrev_source_title={Biol. Membr.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Levina1998883,
author={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},
title={'DNA clearing' from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance},
journal={Membrane and Cell Biology},
year={1998},
volume={12},
number={6},
pages={883-893},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&partnerID=40&md5=693d0527f04b69d04f82f18f75215244},
affiliation={St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation},
abstract={Earlier, we have described the process of active dissociation or 'DNA clearing' from non-covalently bound agents in living mammalian cells. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds DNA in the minor groove tightly but non-covalently, was used for studying the interaction of non-covalently binding agents with DNA. Multiple drug resistance (MDR) in tumour cells is related to the expression of transport proteins that alter the cellular drug transport and distribution. Three different groups of genes (mdr, MRP, and LRP) and their products are implicated in MDR. To obtain new cell lines characterized by enhanced process of active dissociation of non-covalently bound agents from DNA or 'DNA clearing', we carried out step-by-step selection with increasing concentrations of Hoechst 33342. The rodent cell lines hyperresistant to Hoechst 33342 and selected from AA8 were named AA8Hoe-R-1-AA8Hoe-R-10, and the cell lines selected from L cells were called LHoe-R-1-LHoe-R-10. The most resistant of them, AA8Hoe-R-6 and AA8Hoe-R-7, were able to grow in the presence of 80 μm/ml of Hoechst 33342 in the cell culture medium. All mutants were analyzed with the flow cytometric technique and were divided into two different groups. We conclude that the drug resistance of the first group of cell lines was due to changes in transport proteins. The second group of the resistant cell lines was characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. As we believe, the enhanced level of 'DNA clearing' was caused by the amplification of some genes, because the gradual increase of Hoechst resistance in the same cell line resulted from the increase in the ability to remove the dye from DNA. These lines were shown to be also resistant to netropsin.},
correspondence_address1={Levina, V.V.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation; email: levina@ombr.pnpi.spb.ru},
issn={10236597},
coden={MCBIE},
pubmed_id={10512056},
language={English},
abbrev_source_title={Membr. Cell Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Ivanovo1998635,
author={Ivanovo, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},
title={Influence of polyelectrolyte properties of DNA on cytosine protonation},
journal={Molekulyarnaya Biologiya},
year={1998},
volume={32},
number={4},
pages={635-638},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&partnerID=40&md5=15f307298c0ecbf7063bce7528f1f30b},
issn={00268984},
coden={MOBIB},
pubmed_id={9785567},
language={Russian},
abbrev_source_title={Mol. Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Safronova199823,
author={Safronova, I.Yu. and Semenova, E.V.},
title={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},
journal={Microbiology},
year={1998},
volume={67},
number={1},
pages={23-27},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&partnerID=40&md5=492e8c62636367903a8e064e4bf17882},
affiliation={Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},
abstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cistrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in the growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6. © 1998 MAHK Hayka/lnterperiodica Publishing.},
author_keywords={Alcaligenes xylosoxidans subsp. xylosoxidans;  L-malate;  Maleate cis-trans-isomerase;  Maleic acid;  Transformation},
correspondence_address1={Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},
issn={00262617},
language={English},
abbrev_source_title={Microbiology},
document_type={Article},
source={Scopus},
}



@ARTICLE{Safronova199830,
author={Safronova, I.Yu. and Semenova, E.V.},
title={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},
journal={Mikrobiologiya},
year={1998},
volume={67},
number={1},
pages={30-34},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&partnerID=40&md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c},
affiliation={Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},
abstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cisfrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6.},
author_keywords={Alcaligenes xylosoxidans subsp;  L-malate;  Maleate cis-transisomerase;  Maleic acid;  Transformation;  Xylosoxidans},
correspondence_address1={Safronova, I.Yu.; Moscow State University, Vorob'eve gory, Moscow, 119899, Russian Federation},
issn={00263656},
coden={MIKBA},
language={Russian},
abbrev_source_title={Mikrobiologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov199895,
author={Filatov, M.V. and Pantina, R.A. and Noskin, L.A.},
title={Methods for registration of spontaneous DNA instability in mammalian cells},
journal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},
year={1998},
volume={403},
number={1-2},
pages={95-101},
doi={10.1016/S0027-5107(98)00056-6},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&doi=10.1016%2fS0027-5107%2898%2900056-6&partnerID=40&md5=63c03315650103ce0876961fb7f3630c},
affiliation={St. Petersburg Nucl. Phys. Institute, Gatchina, St. Petersburg 188350, Russian Federation},
abstract={A phenomenon of spontaneous DNA instability displays itself as the low level of repair DNA synthesis that takes place during any cell cycle phases. However, there is a problem in detection of very low intensive repair DNA synthesis. This paper suggests two approaches to detect the spontaneous DNA instability. The first method involves a blockade of the DNA gaps sealing by a combination of inhibitors, hydroxyurea and arabinofuranosyl cytosine. An accumulation of single strand gaps leads to production of DNA double strand breaks and results to reproductive inactivation of cells. It was shown that registration of both these events by different methods (such as viscoelastometry of DNA, orthogonal pulse electrophoresis or comet assay for double strand breaks as well as effectiveness of colony growth for cell inactivation) may be used as suitable measure of the spontaneous DNA instability. The second approach bases on photolysis of bromodeoxyuridine incorporated into repair DNA patches during the spontaneous repair DNA synthesis. Long wave UV irradiation of cells containing bromodeoxyuridine labeled DNA stained with Hoechst 33342 causes their inactivation. Experimental results presented confirm that both methods actually detect the spontaneous DNA instability. It takes note of the spontaneous DNA instability varies for cells from different tissues and species and increases during aging.},
author_keywords={DNA synthesis inhibition;  Repair DNA synthesis;  Spontaneous DNA instability},
correspondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Inst., Gatchina, St. Petersburg 188350, Russian Federation; email: filatov@omrb.pnpi.spb.ru},
issn={00275107},
coden={MRFME},
pubmed_id={9726010},
language={English},
abbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Ivanova1998523,
author={Ivanova, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},
title={Influence of polyelectrolyte properties of DNA on cytosine protonation},
journal={Molecular Biology},
year={1998},
volume={32},
number={4},
pages={523-526},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&partnerID=40&md5=913fa635ede6f8418b0b492cae1970a4},
affiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation},
abstract={The polyelectrolyte properties of DNA affect cytosine protonation. The existence of a local region of lower pH in close proximity to the DNA chain is supposed to explain this phenomenon. Such a region was revealed by absorption spectroscopy with the use of Methyl Red (C15H15N3O2). Differences in the protonation of cytosines in different polyelectrolyte environments were detected spectrophotometrically under identical ionic and other conditions. The polyelectrolyte properties of DNA were proved to determine cytosine protonation at physiological pH. The differences in the protonation of cytosines were also observed in the studies of double-stranded high-and low-molecular DNA of the same nucleotide composition at low ionic strengths. The protonation of cytosine is shifted to the lower pH region for the high-molecular DNA as compared with fragments of lower molecular weight.},
author_keywords={Absorption spectroscopy;  Cytosine protonation;  DNA;  Local pH;  Oligonucleotides;  Polyelectrolyte properties},
correspondence_address1={Ivanova, M.A.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: maria@omrb.pnpi.spb.ru},
issn={00268933},
language={English},
abbrev_source_title={Mol. Biol.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Levina1998898,
author={Levina, V.V. and Varfolomeeva, E.Yu. and Drobchenko, E.A. and Tretyakov, A.N. and Klopov, N.V. and Filatov, M.V.},
title={Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines},
journal={Tsitologiya},
year={1998},
volume={40},
number={10},
pages={898-899},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&partnerID=40&md5=b71f1bc0675e0c35e7470ea90070a032},
affiliation={Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},
abstract={The process of active dissociation or «DNA clearing» of not covalently binding agents from DNA in living HeLa cells was shown by the flow cytometry technique. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds tightly but not covalently to DNA in a minor groove, was used as a basic model to study the interaction of not covalently binding agents with DNA. In this paper, we continue to analyse the «DNA clearing» process in the living fibroblasts of different rodent species (mouse, rat, Chinese hamster). The obtained data suggest that the processes of active dissociation or «DNA clearing» of Hoechst 33342 have some common features in all investigated mammalian cells. Nevertheless, some differences in the process were found in these lines. The role of nucleotide excision repair genes in the process of DNA clearing was not established.},
correspondence_address1={Levina, V.V.; Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},
publisher={Maik Nauka-Interperiodica Publishing},
issn={00413771},
coden={TSITA},
pubmed_id={9864821},
language={Russian},
abbrev_source_title={Tsitologiya},
document_type={Article},
source={Scopus},
}



@ARTICLE{Tretyakov1997527,
author={Tretyakov, A.N. and Pantina, R.A. and Kaboev, O.K.},
title={Fast DNA Amplification in Tiny Ultrathin Microplates},
journal={Bioorganicheskaya Khimiya},
year={1997},
volume={23},
number={6},
pages={527-528},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&partnerID=40&md5=f26acee6ab5c8d940ffeac0eb2340e22},
affiliation={Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},
abstract={A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 μm) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 μl samples and a high velocity of heating and cooling of the thermoblock (up to 7°C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.},
author_keywords={Fast DNA amplification;  Microplate;  Polymerase chain reaction},
correspondence_address1={Tretyakov, A.N.; Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},
publisher={Maik Nauka-Interperiodica Publishing},
issn={01323423},
pubmed_id={9265475},
language={Russian},
abbrev_source_title={Bioorganicheskaya Khim.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1995116,
author={Filatov, M.V. and Varfolomeeva, E.Y. and Ivanov, E.I.},
title={Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils},
journal={Biochemical and Molecular Medicine},
year={1995},
volume={55},
number={2},
pages={116-121},
doi={10.1006/bmme.1995.1041},
note={cited By 27},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&doi=10.1006%2fbmme.1995.1041&partnerID=40&md5=adda09c8d4cb05b774fbe164b90edd86},
affiliation={Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},
abstract={This report describes a method for measuring the respiratory burst in neutrophils, based on intracellular oxidation of the reduced ethidium bromide derivative, hydroethidine. Fluorescence of the resultant product quantitatively determined by flow cytofluorometry serves as a measure of the neutrophil ability to generate superoxide radicals. We found that during inflammation some polymorphonuclear leukocytes showed a considerably lower respiratory burst response to phorbol myristate acetate treatment. It was demonstrated that variations in this parameter could be an indicator of the course of inflammation. © 1995 by Academic Press, Inc.},
correspondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},
issn={10773150},
language={English},
abbrev_source_title={BIOCHEM. MOL. MED.},
document_type={Article},
source={Scopus},
}







@ARTICLE{Katunin199466,
author={Katunin, V.I. and Soboleva, N.G. and Makhno, V.I. and Sedelnikova, E.A. and Zhenodarova, S.M. and Kirillov, S.V.},
title={Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction},
journal={Molekulyarnaya Biologiya},
year={1994},
volume={28},
number={1},
pages={66-75},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&partnerID=40&md5=68e2dad0fa12c3f6e33f54dd5db8fb1f},
affiliation={St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},
abstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNA((G(m)AAY(Phe)) with the substitution at the 37 position (tRNA(GAAA)(Phe)), and at both the anticodon (tRNA(GCAG)(Phe)) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(G(m)(AAY)(Phe), tRNA(GAAA)(Phe), tRNA(GCAG)(Phe), and tRNA(-Y)(Phe) with the P site of the 70S ribosome·poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalphy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U) the affinity of tRNA(-Y)(Phe) for the P site of the 70S ribosome is 5 times lower that the affinity of tRNA(G(m)AAY(Phe)) and tRNA(GCAG)(Phe). Thus, in the ribosome the mofified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon- anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome.},
correspondence_address1={Katunin, V.I.; St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},
issn={00268984},
coden={MOBIB},
pubmed_id={8145756},
language={Russian},
abbrev_source_title={MOL. BIOL.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Katunin199451,
author={Katunin, V. and Soboleva, N. and Mahkno, V. and Sedelnikova, E. and Zhenodarova, S. and Kirillov, S.},
title={Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes},
journal={Biochimie},
year={1994},
volume={76},
number={1},
pages={51-57},
doi={10.1016/0300-9084(94)90062-0},
note={cited By 8},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&doi=10.1016%2f0300-9084%2894%2990062-0&partnerID=40&md5=28e38efb03ea6c49c3f74fdc2487ab60},
affiliation={Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation; Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, 142292 Puschino, Moscow Region, Russian Federation},
abstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe with the substitution at position 37 (tRNAGAAAPhe) and at the anticodon(tRNAGCAGPhe). A quantitative study of the interaction of various types of deacylated yeast tRNAPhe (tRNA+YPhe, tRNAGAAAPhe, tRNA-yPhe) with the P site of the [70S ribosome*poly(U)]-complex was carried out at different Mg2+ concentrations and temperatures. The presence and nature of the nucleotide situated at the 3′-end of the anticodon are essential for such interaction in E coli ribosomes. Replacement of thee Y base with the unmodified adenosine decreases the interation enthalpy from 39 kcal/mol to 24 kcal/mol, whereas its removal reduces the interaction enthalpy to 16 kcal/mol. Replacement of the second anticodon nucleotide, adenosine, with cytosine further reduces the enthalphy to 6 kcal/mol, which is typical of tRNA-P site interaction in the absence of poly(U). In the absence of poly(U) the affinity of tRNA-YPhe for the P site of the 70S ribosome is five times lower than the affinity of tRNA+YPhe or tRNAGCAGPhe. Thus, in the ribosome the modified nucleotide stabilizes the codon-anticodon interaction through its stacking interaction with the codon-anticodon base stack. In addition, this decreases the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site. © 1994.},
author_keywords={70S ribosome;  codon-anticodon interaction;  Escherichia coli;  modified nucleotide;  tRNAPhe},
correspondence_address1={Katunin, V.; Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation},
issn={03009084},
coden={BICMB},
pubmed_id={7518255},
language={English},
abbrev_source_title={Biochimie},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov199368,
author={Filatov, M.V. and Bulat, S.A. and Drobchenko, E.A. and Kotlovanova, L.V. and Pantina, R.A. and Semenova, E.V. and Stepanov, S.I. and Tret'iakov, A.N. and Shcherbakova, O.G.},
title={The production of clones of "man x Chinese hamster" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok "chelovek x kitaǐskiǐ khomiachok", soderzhashchikh raznye chasti genoma cheloveka.]},
journal={Tsitologiya},
year={1993},
volume={35},
number={6-7},
pages={68-73},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&partnerID=40&md5=07712067d4e466fb44c89e58dfa85128},
abstract={Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.},
correspondence_address1={Filatov, M.V.},
issn={00413771},
pubmed_id={8266566},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Bulat199374,
author={Bulat, S.A. and Filatov, M.V. and Pantina, R.A.},
title={The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.]},
journal={Tsitologiya},
year={1993},
volume={35},
number={6-7},
pages={74-78},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&partnerID=40&md5=233809336d07245daae6c4fc6a081fa1},
abstract={The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids "human x Chinese hamster" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.},
correspondence_address1={Bulat, S.A.},
issn={00413771},
pubmed_id={8266567},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Luniak199384,
author={Luniak, V.V. and Filatov, M.V. and Ibatullin, F.M. and Timchenko, N.A.},
title={The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel'nosti DARC146.]},
journal={Tsitologiya},
year={1993},
volume={35},
number={5},
pages={84-90},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&partnerID=40&md5=a0bd6d6a1eee3ff3c287dde1598ec2f6},
abstract={The binding of nuclear proteins to a DARC146 DNA fragment is described. The DARC146 was isolated from a complex form of DNA polymerase alpha. BrdUrd substitution experiments indicate that DARC146 can support an autonomous replication in mammalian cells. Three AAA blocks separated by 10 nucleotides were identified in the DARC146 sequence. Measuring electrophoretical mobility under appropriate conditions showed that these AAA blocks form a bent DNA. We have used a synthetic oligonucleotide covering the bent DNA to study the interaction of nuclear proteins with this DNA region. Four DNA-protein complexes with the bent DNA region were registered. One of them is formed by binding nuclear factor p65 to TCTATTA nucleotides. The molecular weight and binding site of p65 are very similar to those of c-myc protein. However, antibodies against c-myc protein exert no effect on the formation of the p65-DNA complex. We suggest that p65 is an unknown nuclear factor.},
correspondence_address1={Luniak, V.V.},
issn={00413771},
pubmed_id={8379011},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}





@ARTICLE{Bazhanov199293,
author={Bazhanov, N.N. and Semenova, E.V. and Ginzburg, L.I.},
title={The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol'nykh revmatoidnym artritom.]},
journal={Terapevticheskii Arkhiv},
year={1992},
volume={64},
number={2},
pages={93-96},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&partnerID=40&md5=cadd0e27379a5b1b7917b210b3fc1a1c},
abstract={As a result of the work carried out, one could decide some problems: to develop standardized points of measuring temperatures of the deep structures of the joints using radiothermography, to establish for the first time the normal radiothermographic values of the temperatures for different joint groups, to recommend radiothermography as an adjuvant method for revealing early signs of the activity of rheumatoid arthritis (RA) associated with OA. Radiothermography is a convenient, cheap, available and an informative enough method, fit for the use in clinical practice to improve the diagnosis and treatment of RA.},
correspondence_address1={Bazhanov, N.N.},
issn={00403660},
pubmed_id={1509395},
language={Russian},
abbrev_source_title={Ter Arkh},
document_type={Article},
source={Scopus},
}





@ARTICLE{Filatov1991115,
author={Filatov, M.V. and Varfolomeev, D.B. and Kotlovanova, L.V.},
title={New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.]},
journal={Tsitologiya},
year={1991},
volume={33},
number={4},
pages={115-120},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&partnerID=40&md5=665c584f8ca0c771026cfbf0048ba0be},
abstract={A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.},
correspondence_address1={Filatov, M.V.},
issn={00413771},
pubmed_id={1803700},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Meerson1991460,
author={Meerson, F.Z. and Malyshev, I.I. and Varfolomeeva, E.I. and Varfolomeev, O.B. and Noskin, A.N.},
title={Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost' iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.]},
journal={Byulleten Eksperimentalnoi Biologii i Meditsiny},
year={1991},
volume={111},
number={5},
pages={460-462},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&partnerID=40&md5=6def35ea402425f2f367f48691d8c2af},
abstract={The effect of adaptation to stress exposure on the resistance of nuclear DNA to damaging effect of one-chain exogenous DNA was investigated by flow cytometric fluorescence method. It was shown that after the administration of one-chain DNA in concentration of 50 micrograms/ml into the nuclei suspension almost half of nuclear DNA was damaged in control. In adaptation this phenomenon was 5.5 times less pronounced. When the one-chain DNA concentration was increased, this protective effect of adaptation remained. The possible mechanism of the DNA-protective effect of adaptation is under consideration.},
correspondence_address1={Meerson, F.Z.},
issn={03659615},
pubmed_id={1878555},
language={Russian},
abbrev_source_title={Biull Eksp Biol Med},
document_type={Article},
source={Scopus},
}



@ARTICLE{Timchenko1990814,
author={Timchenko, N.A. and Shcherbakov, O.G. and Krutyakov, V.M. and Filatov, M.V.},
title={Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex},
journal={Molekulyarnaya Biologiya},
year={1990},
volume={24},
number={3},
pages={814-823},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&partnerID=40&md5=954a167f2eb8cd97e225fe80f81d78bc},
affiliation={B.P. Konstantinov Nuclear Physics Institute, Academy of Sciences of the USSR, Gatchina, Leningrad Region 188350, Russia},
issn={00268984},
coden={MOBIB},
pubmed_id={2169584},
language={Russian},
abbrev_source_title={MOL. BIOL.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Streltsov1990279,
author={Streltsov, P.G. and Filatov, M.V.},
title={A simple method for estimating the rate of repair synthesis in cultured mammalian cells},
journal={Radiobiologiya},
year={1990},
volume={30},
number={2},
pages={279-282},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&partnerID=40&md5=b8496824b416bf690816ecd2ca1277da},
affiliation={B.P. Konstantinov Leningrad Institute for Nuclear Physics, USSR Academy of Sciences, Gatchina, Russia},
issn={00338192},
coden={RADOA},
pubmed_id={1693442},
language={Russian},
abbrev_source_title={RADIOBIOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1990343,
author={Filatov, M.V. and Varfolomeeva Yu., E.},
title={A study of chromatin packing in the mammalian cell nuclei by flow cytometry},
journal={Tsitologiya},
year={1990},
volume={32},
number={4},
pages={343-351},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&partnerID=40&md5=dfa0e0cab0df064bbdf027d880a7be2b},
affiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Leningrad, Russia},
issn={00413771},
coden={TSITA},
language={Russian},
abbrev_source_title={TSITOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Stepanov1989410,
author={Stepanov, S.I. and Semenova, E.V. and Noskin, L.A. and Drobchenko, E.A. and Filatov, M.V. and Kotlovanova, L.V.},
title={Cell-by-cell chromosomal flow cytometric analysis},
journal={Tsitologiya},
year={1989},
volume={31},
number={4},
pages={410-418},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&partnerID=40&md5=06c63d8e6d241bf9aeaca3c56b5c6c29},
affiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},
issn={00413771},
coden={TSITA},
pubmed_id={2756567},
language={Russian},
abbrev_source_title={TSITOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1988999,
author={Filatov, M.V. and Kotlovanova, L.V. and Stepanov, S.I. and Tret'iakov, A.N. and Strel'tsov, P.G.},
title={Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil'nost' khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch'iu protochnoǐ tsitometrii.]},
journal={Tsitologiya},
year={1988},
volume={30},
number={8},
pages={999-1007},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&partnerID=40&md5=e1990ffa33950ed509f57f13cef44d1a},
abstract={The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.},
correspondence_address1={Filatov, M.V.},
issn={00413771},
pubmed_id={3206547},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov19861364,
author={Filatov, M.V. and Pantina, R.A. and Frolova, L.I.},
title={For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.]},
journal={Tsitologiya},
year={1986},
volume={28},
number={12},
pages={1364-1368},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&partnerID=40&md5=8038fcad140efcdbf1a4e95b7d4885ad},
abstract={The Chinese hamster cells V-79 were treated with BUdR during one cell cycle; after that the cells were grown in the medium without BUdR and were irradiated by longwave-UV-light at different time. The cell survival after photolysis was compared with the percentage of metaphase plates with different number of chromosomes containing BUdR. It is concluded that for cell inactivation the presence of only one destroyed chromosome (or its part) is enough.},
correspondence_address1={Filatov, M.V.},
issn={00413771},
pubmed_id={3824524},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Stepanova1986583,
author={Stepanova, S.I. and Vershilova, E.I. and Malinovskiǐ, O.V. and Filatov, M.V.},
title={Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.]},
journal={Tsitologiia},
year={1986},
volume={28},
number={5},
pages={583-586},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&partnerID=40&md5=c5801fa59eddabd92ec6257a507b8d26},
abstract={A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.},
correspondence_address1={Stepanova, S.I.},
issn={00413771},
pubmed_id={3738992},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Semenova1986643,
author={Semenova, E.V. and Maximov, V.N. and Volokitina, M.V. and Egorov, N.S.},
title={Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum},
journal={Mikrobiologiya},
year={1986},
volume={55},
number={4},
pages={643-647},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&partnerID=40&md5=e0eb3de06bc13b567cab07d6a39ce3c5},
affiliation={Moskovskij Gosudarstvennyj Universitet Biologicheskij Fakul'tet, Moskva, Russian Federation},
abstract={The effect of some components of the medium on the growth of Mycobacterium cyaneum B-646 and on the biosynthesis of an exoglycan by the culture was studied. A medium in which the M. cyaneum M variant produced up to 2.2 g of the exopolysaccharide per litre, which was nearly 4 times more than in the original medium, was proposed. The new medium differed from the original one in an elevated content of carbon, iron and potassium (11.2, 5.0 and 1.4 times, respectively) and in a lower phosphorus content (6.7 times). The exopolysaccharide produced by the culture in this medium contained glucose, galactose, fucose and uronic acid. Therefore, its monomeric composition did not depend on the medium used for growing the culture which produced the exopolysaccharide.},
issn={00263656},
coden={MIKBA},
pubmed_id={3773795},
language={Russian},
abbrev_source_title={MIKROBIOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Bresler1984291,
author={Bresler, S.E. and Davidenkova, E.F. and Filatov, M.V. and Shvarts, E.I. and Noskin, L.A.},
title={Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.]},
journal={Radiobiologiya},
year={1984},
volume={24},
number={3},
pages={291-295},
note={cited By 2},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&partnerID=40&md5=dbd6284fdad0eebea33f4423d212ddd6},
abstract={A technique for registration of spontaneous DNA lesions in intact mammalian cells is proposed. Arabinosid cytosine and hydroxyurea were shown to inhibit the repair of gaps spontaneously formed and accumulated in DNA leading to the formation of double-strand DNA breaks and cell death. The rate of spontaneous lesions was different in cells from different animal species and different tissues. A considerable increase in the level of spontaneous lesions was noted with ageing. It is concluded that the level of spontaneous DNA lesions is one of the fundamental biologically important characteristics of a cell.},
correspondence_address1={Bresler, S.E.},
issn={00338192},
pubmed_id={6739729},
language={Russian},
abbrev_source_title={Radiobiologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1984450,
author={Filatov, M.V. and Sheǐkina, T.A.},
title={New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.]},
journal={Tsitologiya},
year={1984},
volume={26},
number={4},
pages={450-457},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&partnerID=40&md5=993c307758a8f3695b9b6f979ad06bf8},
abstract={A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.},
correspondence_address1={Filatov, M.V.},
issn={00413771},
pubmed_id={6740764},
language={Russian},
abbrev_source_title={Tsitologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Egorov1984199,
author={Egorov, N.S. and Grechushkina, N.N. and Botvinko, I.V. and Sviridov, A.F. and Semenova, E.V.},
title={Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.]},
journal={Mikrobiologiya},
year={1984},
volume={53},
number={2},
pages={199-202},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&partnerID=40&md5=8308484a481aa0f50924b9af171d5084},
abstract={Exocellular polysaccharides were synthesized by ten strains of 7 saprotrophic mycobacterial species in parallel with their growth. The cultures grew and produced polysaccharides more rapidly in a medium with glucose than in a medium with n-hexadecane. The polymers are homogeneous acid heteroglycans of the regular structure. All of them contain glucose and some of them contain hexuronic acid. Galactose, fucose, mannose, ramnose and pyruvic acid are found in some of the polysaccharides. The exoglycans of two mycobacterial species contain mannolactylic acid. Beta-glycosidic linkages prevail in most of the polysaccharides.},
correspondence_address1={Egorov, N.S.},
issn={00263656},
pubmed_id={6738386},
language={Russian},
abbrev_source_title={Mikrobiologiia},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov19841208,
author={Filatov, M.V. and Sheikina, T.A.},
title={Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation},
journal={Tsitologiya},
year={1984},
volume={26},
number={10},
pages={1208-1212},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&partnerID=40&md5=bbdd1d28eb7da8606eae844f947424da},
affiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},
issn={00413771},
coden={TSITA},
pubmed_id={6515721},
language={Russian},
abbrev_source_title={TSITOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Botvinko198489,
author={Botvinko, I.V. and Grechushkina, N.N. and Rachinskaia, L.G. and Semenova, E.V. and Egorov, N.S.},
title={Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul'tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.]},
journal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},
year={1984},
number={2},
pages={89-92},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&partnerID=40&md5=58e4dbe1068fbdb5fb8908eaf0c1738c},
abstract={The source of carbon and nitrogen as well as pH of medium influence the possibility of synthesis of the exocellular heteropolysaccharide of Mycobacterium cyaneum and the quantitative harvest of glucan but not its monomer composition. The conditions of mycobacterial growth and glucan synthesis usually do not coincide.},
correspondence_address1={Botvinko, I.V.},
issn={04704606},
pubmed_id={6713026},
language={Russian},
abbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},
document_type={Article},
source={Scopus},
}



@ARTICLE{Oleinikova1983320,
author={Oleinikova, E.A. and Egorov, N.S. and Anoshkina, G.B. and Morozov, A.T. and Botvinko, I.V. and Semenova, E.V.},
title={Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection},
journal={Bulletin of Experimental Biology and Medicine},
year={1983},
volume={95},
number={3},
pages={320-323},
doi={10.1007/BF00830178},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&doi=10.1007%2fBF00830178&partnerID=40&md5=2d05f6b733ea895fd47478957ed6c488},
author_keywords={cell enzymes;  cell reaction;  macrophages;  mycobacterial polysaccharide;  phagocytosis},
publisher={Kluwer Academic Publishers-Plenum Publishers},
issn={00074888},
coden={BEXBA},
language={English},
abbrev_source_title={Bull Exp Biol Med},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1983393,
author={Filatov, M.V. and Noskin, L.A.},
title={Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents},
journal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},
year={1983},
volume={110},
number={2},
pages={393-399},
doi={10.1016/0027-5107(83)90155-0},
note={cited By 6},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&doi=10.1016%2f0027-5107%2883%2990155-0&partnerID=40&md5=618e32c711de3df565c57dec8399c8d5},
affiliation={Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},
abstract={Inhibitors of DNA synthesis 1-β-arabinofuranosylcytosine (Ac) and hydroxyurea (Hu) taken together drastically sensitized human cells to the killing effect of DNA-damaging agents. For UV-irradiation this sensitization depended on the cells′ ability for excision repair. By using viscoelastometric methods of measurement of double-strand breaks (DSB) in the genome, it was established that the first DSB were generated after incubation of the damaged cells in the mixture of inhibitors at about the same dose when sensitization appeared. A scheme is proposed to described molecular events associated with the phenomenon studied. © 1983.},
correspondence_address1={Filatov, M.V.; Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},
issn={00275107},
coden={MRFME},
pubmed_id={6877262},
language={English},
abbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},
document_type={Article},
source={Scopus},
}



@ARTICLE{Belyakova1982593,
author={Belyakova, N.V. and Naryzhnyi, S.N. and Filatov, M.V. and Krutyakov, V.M.},
title={Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin},
journal={Radiobiologiya},
year={1982},
volume={22},
number={5},
pages={593-596},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&partnerID=40&md5=e6f60aadab293185ae5595d6bf4bebdf},
affiliation={B.P. Konstantinov Leningrad Inst. Nucl. Phys., USSR Acad. Sci., Gatchina},
abstract={Analysis was made of the enzymic and structural factors responsible for activation of repair DNA synthesis in γ-irradiated chromatin isolated from rat liver or some human cells. The results obtained prompted us to reduce by 10-12 times the dose of radiation used before. With doses of 30 Gy and 10 Gy, the value of the original repair synthesis was doubled in the chromatin of rat liver and HeLa cells, respectively.},
issn={00338192},
coden={RADOA},
pubmed_id={6294723},
language={Russian},
abbrev_source_title={RADIOBIOLOGIYA},
document_type={Article},
source={Scopus},
}



@ARTICLE{Botvinko198195,
author={Botvinko, I.V. and Grechushkina, N.N. and Semenova, E.V. and Egorov, N.S.},
title={Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.]},
journal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},
year={1981},
number={4},
pages={95-99},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&partnerID=40&md5=2e1d7f3a9923aa76ca56ba13cbc0989d},
correspondence_address1={Botvinko, I.V.},
issn={04704606},
pubmed_id={7236813},
language={Russian},
abbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},
document_type={Article},
source={Scopus},
}



@ARTICLE{Filatov1978751,
author={Filatov, M.V. and Sheikina, T.A.},
title={Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation},
journal={Radiobiologiya},
year={1978},
volume={18},
number={5},
pages={751-754},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&partnerID=40&md5=4218fc5755a7f1beb190156f709214c7},
affiliation={B.P. Konstantinov Inst. Nucl. Phys., USSR Acad. Sci., Leningrad},
abstract={Interphase death of HeLa cells was assessed by the ability of cells to be stained with trypan blue after γ-irradiation with doses from 50 to 2000 krad. A quantitative relationship between average life span of cells and radiation dose has been shown. A decrease of the postirradiation temperature and an increase of the serum content in the growth medium before irradiation have been found to delay the interphase death. Adhesive properties of cells were damaged immediately after exposure.},
issn={00338192},
coden={RADOA},
pubmed_id={715202},
language={Russian},
abbrev_source_title={RADIOBIOLOGIYA},
document_type={Article},
source={Scopus},
}
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In order to keep using Mendeley with BibBase past April\n        14th, you need to:\n        <ol>\n          <li>renew the authorization for BibBase on Mendeley, and</li>\n          <li>update the BibBase URL\n            in your page the same way you did when you initially set up\n            this page.\n          </li>\n        </ol>\n      </p>\n\n      <p>\n        <a href=\"http://bibbase.org/cgi-bin/mendeley2/get.py\">\n          <i class=\"fa fa-angle-double-right\"></i>\n          Fix it now</a>\n      </p>\n    </div>\n\n  </div>\n\n\n  <div id=\"bibbase_body\">\n    \n  \n  <div class=\"bibbase_group_whole\" id=\"group_2019\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2019')\"\n      onkeypress=\"toggleGroup('group_2019')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2019</span>\n      <span class=\"bibbase_group_count\">\n        \n        (7)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&amp;doi=10.1016%2fj.bbrc.2019.09.116&amp;partnerID=40&amp;md5=a06fb06bdcf8a8d38c05dce20a1ca016\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&amp;doi=10.1016%2fj.bbrc.2019.09.116&amp;partnerID=40&amp;md5=a06fb06bdcf8a8d38c05dce20a1ca016', event);\">\n    Effect of alpha-lactalbumin and lactoferrin oleic acid complexes on chromatin structural organization.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Biochemical and Biophysical Research Communications</i>, 520(1): 136-139.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&amp;doi=10.1016%2fj.bbrc.2019.09.116&amp;partnerID=40&amp;md5=a06fb06bdcf8a8d38c05dce20a1ca016\"\n     onclick=\"log_download('anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072708276&amp;doi=10.1016%2fj.bbrc.2019.09.116&amp;partnerID=40&amp;md5=a06fb06bdcf8a8d38c05dce20a1ca016', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effect of alpha-lactalbumin and lactoferrin oleic acid complexes on chromatin structural organization [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.bbrc.2019.09.116\"\n     onclick=\"log_download('anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019', 'http://doi.org/10.1016/j.bbrc.2019.09.116', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>4 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-effectofalphalactalbuminandlactoferrinoleicacidcomplexesonchromatinstructuralorganization-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  This work focuses on the study of multimeric alpha-lactalbumin oleic acid and lactoferrin oleic acid complexes. The purpose of the research is to study possible mechanisms involved in their pro-apoptotic activities, as seen in some tumor cell cultures. Complexes featuring oleic acid (OA) with human alpha-lactalbumin (hAl) or with bovine alpha-lactalbumin (bAl), and human lactoferrin (hLf) were investigated using small-angle neutron scattering (SANS). It was shown that while alpha-lactalbumin protein complexes were formed on the surface of polydisperse OA micelles, the lactoferrin complexes comprised a monodisperse system of nanoscale particles. Both hAl and hLf complexes appeared to interact with the chromatin of isolated nuclei affecting chromatin structural organization. The possible roles of these processes in the specific anti-tumor activity of these complexes are discussed. © 2019 Elsevier Inc.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&amp;doi=10.1016%2fj.msec.2019.109945&amp;partnerID=40&amp;md5=71eb20737220e772bd8bc01af8589292\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&amp;doi=10.1016%2fj.msec.2019.109945&amp;partnerID=40&amp;md5=71eb20737220e772bd8bc01af8589292', event);\">\n    In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Materials Science and Engineering C</i>, 104.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&amp;doi=10.1016%2fj.msec.2019.109945&amp;partnerID=40&amp;md5=71eb20737220e772bd8bc01af8589292\"\n     onclick=\"log_download('anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069601872&amp;doi=10.1016%2fj.msec.2019.109945&amp;partnerID=40&amp;md5=71eb20737220e772bd8bc01af8589292', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"In vitro and in vivo study of the toxicity of fullerenols С60, С70 and С120О obtained by an original two step method [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.msec.2019.109945\"\n     onclick=\"log_download('anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019', 'http://doi.org/10.1016/j.msec.2019.109945', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>4 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-invitroandinvivostudyofthetoxicityoffullerenols6070and120obtainedbyanoriginaltwostepmethod-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The toxicity of C60(OH)30, C70(OH)30, and C120O(OH)n fullerenols, prepared by a new original method, has been studied. This method allowed us to obtain high-purity fullerenols and eliminate the risks of synthesis of preparations containing insoluble fractions contaminated with impurities such as fullerenes not completely reacted by hydroxylation. All fullerenols were detected inside cultured cells. The MTT assay as well as the analysis of apoptosis and cell cycle showed that С60(ОН)30 and С70(OH)30 are non-toxic for cultured V79 и HeLa cells at concentrations exceeding physiological levels by an order of magnitude. С120O(OH)n caused low toxicity. Studies in Drosophila melanogaster showed that any preparations used did not result in a decreased lifespan or in behavior abnormalities in flies. © 2019 Elsevier B.V.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&amp;doi=10.1107%2fS160057671900921X&amp;partnerID=40&amp;md5=3e50710e2425c6cdf7917c01d498f65d\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&amp;doi=10.1107%2fS160057671900921X&amp;partnerID=40&amp;md5=3e50710e2425c6cdf7917c01d498f65d', event);\">\n    Small-angle neutron scattering (SANS) and spinecho SANS measurements reveal the logarithmic fractal structure of the large-scale chromatin organization in HeLa nuclei.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Journal of Applied Crystallography</i>, 52(4): 844-853.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&amp;doi=10.1107%2fS160057671900921X&amp;partnerID=40&amp;md5=3e50710e2425c6cdf7917c01d498f65d\"\n     onclick=\"log_download('anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069964857&amp;doi=10.1107%2fS160057671900921X&amp;partnerID=40&amp;md5=3e50710e2425c6cdf7917c01d498f65d', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Small-angle neutron scattering (SANS) and spinecho SANS measurements reveal the logarithmic fractal structure of the large-scale chromatin organization in HeLa nuclei [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1107/S160057671900921X\"\n     onclick=\"log_download('anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019', 'http://doi.org/10.1107/S160057671900921X', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-smallangleneutronscatteringsansandspinechosansmeasurementsrevealthelogarithmicfractalstructureofthelargescalechromatinorganizationinhelanuclei-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  This paper reports on the two-scale fractal structure of chromatin organization in the nucleus of the HeLa cell. Two neutron scattering methods, small-angle neutron scattering (SANS) and spin-echo SANS, are used to unambiguously identify the large-scale structure as being a logarithmic fractal with the correlation function (r) - ln(r/E). The smaller-scale structural level is shown to be a volume fractal with dimension DF = 2.41. By definition, the volume fractal is self-similar at different scales, while the logarithmic fractal is hierarchically changed upon scaling. As a result, the logarithmic fractal is more compact than the volume fractal but still has a rather high surface area, which provides accessibility at all length scales. Apparently such bi-fractal chromatin organization is the result of an evolutionary process of optimizing the compactness and accessibility of gene packing. As they are in a water solution, the HeLa nuclei tend to agglomerate over time. The large-scale logarithmic fractal structure of chromatin provides the HeLa nucleus with the possibility of penetrating deeply into the adjacent nucleus during the agglomeration process. The interpenetration phenomenon of the HeLa nuclei shows that the chromatin-free space of one nucleus is not negligible but is as large as the volume occupied by chromatin itself. It is speculated that it is the logarithmic fractal architecture of chromatin that provides a comfortable compartment for this most important function of the cell. © 2019 International Union of Crystallography.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&amp;doi=10.1134%2fS1990519X1903009X&amp;partnerID=40&amp;md5=89a5f8a9f3aba34d2c3fe326d4ff81c1\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&amp;doi=10.1134%2fS1990519X1903009X&amp;partnerID=40&amp;md5=89a5f8a9f3aba34d2c3fe326d4ff81c1', event);\">\n    Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Pantina, R.; Varfolomeeva, E.; Burdakov, V.; Landa, S.; Bayramukov, V.; Kovalev, R.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 13(3): 188-197.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&amp;doi=10.1134%2fS1990519X1903009X&amp;partnerID=40&amp;md5=89a5f8a9f3aba34d2c3fe326d4ff81c1\"\n     onclick=\"log_download('pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&amp;doi=10.1134%2fS1990519X1903009X&amp;partnerID=40&amp;md5=89a5f8a9f3aba34d2c3fe326d4ff81c1', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X1903009X\"\n     onclick=\"log_download('pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019', 'http://doi.org/10.1134/S1990519X1903009X', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('pantina-varfolomeeva-burdakov-landa-bayramukov-kovalev-filatov-temporalparametersofp53gfpproteintransferviaexosomesincoculturedhek293andgfphek293cells-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Pantina2019188,\r\nauthor={Pantina, R.A. and Varfolomeeva, E.Y. and Burdakov, V.S. and Landa, S.B. and Bayramukov, V.Y. and Kovalev, R.A. and Filatov, M.V.},\r\ntitle={Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells},\r\njournal={Cell and Tissue Biology},\r\nyear={2019},\r\nvolume={13},\r\nnumber={3},\r\npages={188-197},\r\ndoi={10.1134/S1990519X1903009X},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067858794&amp;doi=10.1134%2fS1990519X1903009X&amp;partnerID=40&amp;md5=89a5f8a9f3aba34d2c3fe326d4ff81c1},\r\naffiliation={St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, 191036, Russian Federation},\r\nabstract={Abstract: In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population. © 2019, Pleiades Publishing, Ltd.},\r\nauthor_keywords={cell-to-cell communication;  exosomes;  Hek293 cell line;  p53-GFP},\r\ncorrespondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov InstituteRussian Federation; email: fil_53@mail.ru},\r\npublisher={Pleiades Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Abstract: In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population. © 2019, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&amp;doi=10.1371%2fjournal.pone.0211980&amp;partnerID=40&amp;md5=b33be3ba1c58420838f9b1c65e11c6ae\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&amp;doi=10.1371%2fjournal.pone.0211980&amp;partnerID=40&amp;md5=b33be3ba1c58420838f9b1c65e11c6ae', event);\">\n    Abnormal activity of transcription factors gli in high-grade gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Volnitskiy, A.; Shtam, T.; Burdakov, V.; Kovalev, R.; Konev, A.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>PLoS ONE</i>, 14(2).  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&amp;doi=10.1371%2fjournal.pone.0211980&amp;partnerID=40&amp;md5=b33be3ba1c58420838f9b1c65e11c6ae\"\n     onclick=\"log_download('volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&amp;doi=10.1371%2fjournal.pone.0211980&amp;partnerID=40&amp;md5=b33be3ba1c58420838f9b1c65e11c6ae', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Abnormal activity of transcription factors gli in high-grade gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1371/journal.pone.0211980\"\n     onclick=\"log_download('volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019', 'http://doi.org/10.1371/journal.pone.0211980', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>1 download</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('volnitskiy-shtam-burdakov-kovalev-konev-filatov-abnormalactivityoftranscriptionfactorsgliinhighgradegliomas-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Volnitskiy2019,\r\nauthor={Volnitskiy, A. and Shtam, T. and Burdakov, V. and Kovalev, R. and Konev, A. and Filatov, M.},\r\ntitle={Abnormal activity of transcription factors gli in high-grade gliomas},\r\njournal={PLoS ONE},\r\nyear={2019},\r\nvolume={14},\r\nnumber={2},\r\ndoi={10.1371/journal.pone.0211980},\r\nart_number={e0211980},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061246108&amp;doi=10.1371%2fjournal.pone.0211980&amp;partnerID=40&amp;md5=b33be3ba1c58420838f9b1c65e11c6ae},\r\naffiliation={Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre &quot;Kurchatov Institute, Gatchina, Russian Federation; N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Pesochnyj, Leningradskaya, Russian Federation},\r\nabstract={Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity of transcription factors gli in high-grade gliomas and their role in maintenance of stem cell state and glioma cell survival. 20 glioma cell lines and a sample of a normal adult brain tissue were used in the present study. We found the expression of gli target genes, including GLI1 and FOXM1, in all tested glioma cell lines, but not in the normal tissue. Interestingly, the expression of gli target genes in some glioma cell lines was observed together with a high level of their transcriptional repressor, Gli3R. Knockdown of GLI3 in one of these lines resulted in decrease of gli target gene expression. These data suggest that Gli3R does not prevent the gli target genes transcription, and gli3 acts in glioma cells more as an activator, than a repressor of transcription. We observed that gli regulated the expression of such genes, as SOX2 or OCT4 that maintain stem cell state, and TET1, involving in DNA demethylation. Treatment with GANT61 or siRNA against GLI1, GLI2, or GLI3 could result in complete glioma cell death, while cyclopamine had a weaker and line-specific effect on glioma cell survival. Thus, the gli transcription factors are abnormally active in high-grade gliomas, regulate expression of genes, maintaining the stem cell state, and contribute to glioma cell survival. © 2019 Volnitskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},\r\ncorrespondence_address1={Volnitskiy, A.; Petersburg Nuclear Physics Institute named by B.P. Konstantinov, National Research Centre &quot;Kurchatov InstituteRussian Federation; email: voln.a@yandex.ru},\r\npublisher={Public Library of Science},\r\nissn={19326203},\r\ncoden={POLNC},\r\npubmed_id={30730955},\r\nlanguage={English},\r\nabbrev_source_title={PLoS ONE},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity of transcription factors gli in high-grade gliomas and their role in maintenance of stem cell state and glioma cell survival. 20 glioma cell lines and a sample of a normal adult brain tissue were used in the present study. We found the expression of gli target genes, including GLI1 and FOXM1, in all tested glioma cell lines, but not in the normal tissue. Interestingly, the expression of gli target genes in some glioma cell lines was observed together with a high level of their transcriptional repressor, Gli3R. Knockdown of GLI3 in one of these lines resulted in decrease of gli target gene expression. These data suggest that Gli3R does not prevent the gli target genes transcription, and gli3 acts in glioma cells more as an activator, than a repressor of transcription. We observed that gli regulated the expression of such genes, as SOX2 or OCT4 that maintain stem cell state, and TET1, involving in DNA demethylation. Treatment with GANT61 or siRNA against GLI1, GLI2, or GLI3 could result in complete glioma cell death, while cyclopamine had a weaker and line-specific effect on glioma cell survival. Thus, the gli transcription factors are abnormally active in high-grade gliomas, regulate expression of genes, maintaining the stem cell state, and contribute to glioma cell survival. © 2019 Volnitskiy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&amp;doi=10.15789%2f1563-0625-2019-3-479-486&amp;partnerID=40&amp;md5=3491db5f8ebdda1e86d4978600a5e9e7\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&amp;doi=10.15789%2f1563-0625-2019-3-479-486&amp;partnerID=40&amp;md5=3491db5f8ebdda1e86d4978600a5e9e7', event);\">\n    Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Zinchenko, Y.; Starshinova, A.; Filatov, M.; Denisova, N.; Landa, S.; Burdakov, V.;  and Yablonskiy, P.\n</span>\n\n  \n\n</span>\n\n  <i>Medical Immunology (Russia)</i>, 21(3): 479-486.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&amp;doi=10.15789%2f1563-0625-2019-3-479-486&amp;partnerID=40&amp;md5=3491db5f8ebdda1e86d4978600a5e9e7\"\n     onclick=\"log_download('zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&amp;doi=10.15789%2f1563-0625-2019-3-479-486&amp;partnerID=40&amp;md5=3491db5f8ebdda1e86d4978600a5e9e7', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.15789/1563-0625-2019-3-479-486\"\n     onclick=\"log_download('zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019', 'http://doi.org/10.15789/1563-0625-2019-3-479-486', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('zinchenko-starshinova-filatov-denisova-landa-burdakov-yablonskiy-autoimmuneoriginofsarcoidosisdeterminationofspecificimmunecomplexesinpatientswithrespiratorysarcoidosis-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Zinchenko2019479,\r\nauthor={Zinchenko, Yu.S. and Starshinova, A.A. and Filatov, M.V. and Denisova, N.V. and Landa, S.B. and Burdakov, V.S. and Yablonskiy, P.K.},\r\ntitle={Autoimmune origin of sarcoidosis: Determination of specific immune complexes in patients with respiratory sarcoidosis [АУТОИММУННАЯ ПРИРОДА САРКОИДОЗА: ОПРЕДЕЛЕНИЕ СПЕЦИФИЧЕСКИХ ИММУННЫХ КОМПЛЕКСОВ У БОЛЬНЫХ САРКОИДОЗОМ ОРГАНОВ ДЫХАНИЯ]},\r\njournal={Medical Immunology (Russia)},\r\nyear={2019},\r\nvolume={21},\r\nnumber={3},\r\npages={479-486},\r\ndoi={10.15789/1563-0625-2019-3-479-486},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070191457&amp;doi=10.15789%2f1563-0625-2019-3-479-486&amp;partnerID=40&amp;md5=3491db5f8ebdda1e86d4978600a5e9e7},\r\naffiliation={St. Petersburg State Research Institute of Phthisiopulmonology, St. Petersburg, Russian Federation; St. Petersburg B. Konstantinov Institute of Nuclear Physics, National Research Center “Kurchatov Institute, Gatchina, Leningrad Region, Russian Federation; St. Petersburg State University, St. Petersburg, Russian Federation},\r\nabstract={The etiology of sarcoidosis is not completely understood. A hypothesis exists about the relationship between sarcoidosis and a complex of pathological autoimmune reactions that occur under the influence of triggering factors. In this study, specific immune complexes in the blood plasma of patients have been determined, which can indirectly reveal the causes of the disease. The study included 33 patients with lung sarcoidosis (I group), compared to 24 healthy donors who served as a control group (II group). The patients underwent standard examination. Their blood plasma was investigated by the dynamic light scattering method with addition of tuberculosis antigens (ESAT-6/SFP-10) and “lung healthy tissue extract”. Statistical analysis was performed using the Statistica 7.0 program. Test results were considered significant at p &lt; 0.05. Аccording to the data obtained, addition of ESAT-6/SFP-10 to patient’s blood plasma almost did not lead to the formation of immune complexes in most samples. Meanwhile, development of such complexes after addition of “lung tissue extract” was revealed in all the patients. The immune complexes were not detected in any donor from control group after stimulation with both kinds of antigens (p &lt; 0.01). The data on distinct formation of immune complexes with the addition of “lung healthy tissue extract” in patients with lung sarcoidosis may be considered an indirect evidence for occurrence of autoimmune reaction under the influence of some pathogenic factors. Absence of de novo immune complex formation after addition of tuberculosis antigens (ESAT-6/SFP-10) makes it unlikely any direct effects of tuberculosis bacteria upon development of sarcoidosis. © 2019, SPb RAACI.},\r\nauthor_keywords={Autoimmunity;  Dynamic light scattering method;  Sarcoidosis;  Specific immune complexes},\r\ncorrespondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},\r\npublisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},\r\nissn={15630625},\r\nlanguage={Russian},\r\nabbrev_source_title={Med. Immunol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The etiology of sarcoidosis is not completely understood. A hypothesis exists about the relationship between sarcoidosis and a complex of pathological autoimmune reactions that occur under the influence of triggering factors. In this study, specific immune complexes in the blood plasma of patients have been determined, which can indirectly reveal the causes of the disease. The study included 33 patients with lung sarcoidosis (I group), compared to 24 healthy donors who served as a control group (II group). The patients underwent standard examination. Their blood plasma was investigated by the dynamic light scattering method with addition of tuberculosis antigens (ESAT-6/SFP-10) and “lung healthy tissue extract”. Statistical analysis was performed using the Statistica 7.0 program. Test results were considered significant at p < 0.05. Аccording to the data obtained, addition of ESAT-6/SFP-10 to patient’s blood plasma almost did not lead to the formation of immune complexes in most samples. Meanwhile, development of such complexes after addition of “lung tissue extract” was revealed in all the patients. The immune complexes were not detected in any donor from control group after stimulation with both kinds of antigens (p < 0.01). The data on distinct formation of immune complexes with the addition of “lung healthy tissue extract” in patients with lung sarcoidosis may be considered an indirect evidence for occurrence of autoimmune reaction under the influence of some pathogenic factors. Absence of de novo immune complex formation after addition of tuberculosis antigens (ESAT-6/SFP-10) makes it unlikely any direct effects of tuberculosis bacteria upon development of sarcoidosis. © 2019, SPb RAACI.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&amp;doi=10.15789%2f1563-0625-2019-2-269-278&amp;partnerID=40&amp;md5=a90c16d4007b2884eb6918641062312c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&amp;doi=10.15789%2f1563-0625-2019-2-269-278&amp;partnerID=40&amp;md5=a90c16d4007b2884eb6918641062312c', event);\">\n    Diagnostic value of specific immune complexes in detection of active tuberculosis infection.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Starshinova, A.; Istomina, E.; Zinchenko, Y.; Filatov, M.; Landa, S.; Burdakov, V.; Belyaeva, E.; Nazarenko, M.; Sapozhnikova, N.;  and Yablonkiy, P.\n</span>\n\n  \n\n</span>\n\n  <i>Medical Immunology (Russia)</i>, 21(2): 269-278.  2019.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&amp;doi=10.15789%2f1563-0625-2019-2-269-278&amp;partnerID=40&amp;md5=a90c16d4007b2884eb6918641062312c\"\n     onclick=\"log_download('starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&amp;doi=10.15789%2f1563-0625-2019-2-269-278&amp;partnerID=40&amp;md5=a90c16d4007b2884eb6918641062312c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Diagnostic value of specific immune complexes in detection of active tuberculosis infection [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.15789/1563-0625-2019-2-269-278\"\n     onclick=\"log_download('starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019', 'http://doi.org/10.15789/1563-0625-2019-2-269-278', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('starshinova-istomina-zinchenko-filatov-landa-burdakov-belyaeva-nazarenko-etal-diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection-2019')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Starshinova2019269,\r\nauthor={Starshinova, A.A. and Istomina, E.V. and Zinchenko, Yu.S. and Filatov, M.V. and Landa, S.B. and Burdakov, V.S. and Belyaeva, E.N. and Nazarenko, M.M. and Sapozhnikova, N.V. and Yablonkiy, P.K.},\r\ntitle={Diagnostic value of specific immune complexes in detection of active tuberculosis infection},\r\njournal={Medical Immunology (Russia)},\r\nyear={2019},\r\nvolume={21},\r\nnumber={2},\r\npages={269-278},\r\ndoi={10.15789/1563-0625-2019-2-269-278},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067951328&amp;doi=10.15789%2f1563-0625-2019-2-269-278&amp;partnerID=40&amp;md5=a90c16d4007b2884eb6918641062312c},\r\naffiliation={St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, St. Petersburg, 191036, Russian Federation},\r\nabstract={The diagnostics of tuberculosis infection, including immunological methods, evolved significant changes over time. Introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to differentiate between these conditions, thus requiring development and implementation of new diagnostic approaches. A prospective study enrolling two groups of patients was conducted as follows: group I (n = 50) included patients with verified pulmonary tuberculosis, MBT (+), whereas group II (n = 15) consisted of subjects with LTI, and control group was represented by healthy subjects (n = 14). The entire examination protocol included clinical, radiological, bacteriological, and immunological methods (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskin test). Immune complexes were determined in all patients and healthy individuals by means of dynamic light scattering after the in vitro addition of specific antigens (ESAT-6 and SFP-10 peptides). The data obtained have shown low informativity of clinical methods in diagnostics of pulmonary tuberculosis. In the presence of characteristic X-radiographic changes, bacteriological verification of tuberculosis was proven only in 46% of cases. Usage of various immunological tests allowed to obtain positive results in 84-90% of cases, along with 100% positivity in subjects with LTI. Detection of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases, and to identify the high-risk group for development of active tuberculosis among the subjects with latent tuberculosis infection. Conclusions: the obtained data may be applied both for diagnosis of active tuberculosis in the absence of verified diagnosis, but also enable identification of a high-risk group for development of the disease in subjects with latent tuberculosis infection. © 2019, SPb RAACI.},\r\nauthor_keywords={Dynamic light scattering method;  Immunological tests;  Latent tuberculosis infection;  Test with allergen tuberculosis recombinant;  Tuberculosis},\r\ncorrespondence_address1={Zinchenko, Yu.S.; St. Petersburg State Research Institute of Phthisiopulmonology, Ligovsky Ave., 2-4, Russian Federation; email: Ulia-Zinchenko@yandex.ru},\r\npublisher={Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)},\r\nissn={15630625},\r\nlanguage={Russian},\r\nabbrev_source_title={Med. Immunol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The diagnostics of tuberculosis infection, including immunological methods, evolved significant changes over time. Introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to differentiate between these conditions, thus requiring development and implementation of new diagnostic approaches. A prospective study enrolling two groups of patients was conducted as follows: group I (n = 50) included patients with verified pulmonary tuberculosis, MBT (+), whereas group II (n = 15) consisted of subjects with LTI, and control group was represented by healthy subjects (n = 14). The entire examination protocol included clinical, radiological, bacteriological, and immunological methods (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskin test). Immune complexes were determined in all patients and healthy individuals by means of dynamic light scattering after the in vitro addition of specific antigens (ESAT-6 and SFP-10 peptides). The data obtained have shown low informativity of clinical methods in diagnostics of pulmonary tuberculosis. In the presence of characteristic X-radiographic changes, bacteriological verification of tuberculosis was proven only in 46% of cases. Usage of various immunological tests allowed to obtain positive results in 84-90% of cases, along with 100% positivity in subjects with LTI. Detection of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases, and to identify the high-risk group for development of active tuberculosis among the subjects with latent tuberculosis infection. Conclusions: the obtained data may be applied both for diagnosis of active tuberculosis in the absence of verified diagnosis, but also enable identification of a high-risk group for development of the disease in subjects with latent tuberculosis infection. © 2019, SPb RAACI.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2018\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2018')\"\n      onkeypress=\"toggleGroup('group_2018')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2018</span>\n      <span class=\"bibbase_group_count\">\n        \n        (9)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&amp;doi=10.1007%2fs12026-018-9052-1&amp;partnerID=40&amp;md5=5924fbaf3fd914e3c844ebfe2c48893c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&amp;doi=10.1007%2fs12026-018-9052-1&amp;partnerID=40&amp;md5=5924fbaf3fd914e3c844ebfe2c48893c', event);\">\n    Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Immunologic Research</i>, 66(6): 737-743.  2018.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&amp;doi=10.1007%2fs12026-018-9052-1&amp;partnerID=40&amp;md5=5924fbaf3fd914e3c844ebfe2c48893c\"\n     onclick=\"log_download('anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058622696&amp;doi=10.1007%2fs12026-018-9052-1&amp;partnerID=40&amp;md5=5924fbaf3fd914e3c844ebfe2c48893c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/s12026-018-9052-1\"\n     onclick=\"log_download('anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018', 'http://doi.org/10.1007/s12026-018-9052-1', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-specificfeaturesofimmunecomplexesinpatientswithsarcoidosisandpulmonarytuberculosis-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data on the etiology of the disease. The exclusion of tuberculosis in every patient with suspected sarcoidosis is a mandatory stage of diagnosis, especially in countries with a high burden of tuberculosis. A prospective study was conducted with two groups of patients: group I (n = 50)—patients with pulmonary sarcoidosis established according to standard criteria; group II (n = 28)—patients with pulmonary tuberculosis with bacterial excretion. The control group (n = 24) was presented by healthy subjects. The examination complex included x-ray, bacteriological, immunological (Mantoux test with 2 TE, TB.SPOT test), and histological methods. All patients and healthy subjects were assessed for immune complexes with the use of the dynamic light scattering (DLS) method and adding of “healthy lung tissue extract” antigens and specific tuberculosis antigens ESAT-6 and SFP-10 in vitro. Significant differences were found in determining specific immune complexes in patients with pulmonary sarcoidosis and pulmonary tuberculosis. Registration of specific immune complex formation with “healthy lung tissue extract” in 100% cases may indicate the autoimmune nature of sarcoidosis. The absence of the immune complex formation in response to ESAT-6/SFP-10 antigens can be used for the differential diagnosis of two diseases. The diagnostic significance of the DLS method was 100% for sarcoidosis and 92.2% for tuberculosis. The data obtained in the study allows not only understanding the etiology of sarcoidosis, but also obtaining new criteria for the differential diagnosis of tuberculosis and pulmonary sarcoidosis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&amp;doi=10.1134%2fS1990519X18060068&amp;partnerID=40&amp;md5=1a3891a94ef69f0b40bda4c79c6c8dfe\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&amp;doi=10.1134%2fS1990519X18060068&amp;partnerID=40&amp;md5=1a3891a94ef69f0b40bda4c79c6c8dfe', event);\">\n    Searching for Specific Markers of Glioblastoma: Analysis of Glioblastoma Cell Proteoforms.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 12(6): 455-459.  2018.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&amp;doi=10.1134%2fS1990519X18060068&amp;partnerID=40&amp;md5=1a3891a94ef69f0b40bda4c79c6c8dfe\"\n     onclick=\"log_download('anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059341183&amp;doi=10.1134%2fS1990519X18060068&amp;partnerID=40&amp;md5=1a3891a94ef69f0b40bda4c79c6c8dfe', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Searching for Specific Markers of Glioblastoma: Analysis of Glioblastoma Cell Proteoforms [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X18060068\"\n     onclick=\"log_download('anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018', 'http://doi.org/10.1134/S1990519X18060068', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-searchingforspecificmarkersofglioblastomaanalysisofglioblastomacellproteoforms-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Abstract: Using the sectional analysis of two-dimensional electrophoretic gels with liquid chromatography-mass spectrometry, proteoform profiles for individual genes expressed in cancer (glioblastoma) and normal (FLEH) cells were obtained. Profiles of more than 5000 genes were analyzed. It turned out that many genes encoding potential biomarkers of glioblastoma are characterized by sets of proteoforms that are different in normal and cancer cells. These proteoforms could be sources of highly specific markers and targets for therapy. Using a section analysis of two-dimensional electrophoretic gels with liquid chromatography by mass spectrometry, proteoform profiles were obtained for individual genes expressed in cancer (glioblastoma) and normal (FLEH) cells. Profiles of more than 5000 genes were analyzed. It turned out that many genes encoding potential biomarkers of glioblastoma are characterized by sets of proteoforms, which are different in normal and cancer cells. These proteoforms could be sources of highly specific markers and targets for therapy. © 2018, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&amp;doi=10.1134%2fS1990793118030065&amp;partnerID=40&amp;md5=bef4391cf3e6455e8b4c238f9b0e484a\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&amp;doi=10.1134%2fS1990793118030065&amp;partnerID=40&amp;md5=bef4391cf3e6455e8b4c238f9b0e484a', event);\">\n    Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Chernous, S.; Shagimuratov, I.; Ievenko, I.; Filatov, M.; Efishov, I.; Shvets, M.;  and Kalitenkov, N.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Physical Chemistry B</i>, 12(3): 562-567.  2018.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&amp;doi=10.1134%2fS1990793118030065&amp;partnerID=40&amp;md5=bef4391cf3e6455e8b4c238f9b0e484a\"\n     onclick=\"log_download('chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&amp;doi=10.1134%2fS1990793118030065&amp;partnerID=40&amp;md5=bef4391cf3e6455e8b4c238f9b0e484a', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990793118030065\"\n     onclick=\"log_download('chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018', 'http://doi.org/10.1134/S1990793118030065', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('chernous-shagimuratov-ievenko-filatov-efishov-shvets-kalitenkov-auroralperturbationsasanindicatorofionosphereimpactonnavigationsignals-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Chernous2018562,\r\nauthor={Chernous, S.A. and Shagimuratov, I.I. and Ievenko, I.B. and Filatov, M.V. and Efishov, I.I. and Shvets, M.V. and Kalitenkov, N.V.},\r\ntitle={Auroral Perturbations as an Indicator of Ionosphere Impact on Navigation Signals},\r\njournal={Russian Journal of Physical Chemistry B},\r\nyear={2018},\r\nvolume={12},\r\nnumber={3},\r\npages={562-567},\r\ndoi={10.1134/S1990793118030065},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050076158&amp;doi=10.1134%2fS1990793118030065&amp;partnerID=40&amp;md5=bef4391cf3e6455e8b4c238f9b0e484a},\r\naffiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Kaliningrad Branch, Russian Academy of Sciences, Kaliningrad, 236017, Russian Federation; Institute for Cosmophysical Research and Aeronomy, Siberian Branch, Russian Academy of Sciences, Yakutsk, 677098, Russian Federation; Murmansk State Technical University, Sportivnaya 13, Murmansk, 183010, Russian Federation; Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russian Federation},\r\nabstract={A comparative analysis of total electron content (TEC) fluctuations and auroral activity, which characterizes the polar ionosphere during periods of substorm activity, is performed. The analysis is based on GPS/GLONASS observations at auroral and subauroral stations and data on auroral emissions obtained at the Yakutsk subauroral station and the Poker Flat (Alaska) auroral station. A detailed analysis is carried out for the storm on January 7, 2015. During this event, a sharp increase in the auroral activity and in TEC fluctuations from 09 to 13 UT was observed. A similarity of the dynamics of the auroral oval and the space–time distribution of TEC fluctuations associated with ionospheric irregularities in the oval is demonstrated. There is a close correlation between the positions of the auroral oval and the irregularity oval. The positions of the auroral oval, predicted by the NORUSKA Russian–Norwegian model, and of the TEC irregularity oval are compared. In general, the positions of the ovals are close to each other. The existing discrepancy may be due to the fact that the auroral oval is projected to an altitude of 110 km, whereas the irregularity oval, to an altitude of 450 km, and that the curvature of the magnetic force lines is not taken into account. The influence of auroral disturbances on navigational measurements manifests itself through the fact that, during disturbances, faults and failures in the operation of navigation equipment at auroral and subauroral stations increase. © 2018, Pleiades Publishing, Ltd.},\r\nauthor_keywords={auroral oval;  auroral perturbations;  GPS/GLONASS;  TEC fluctuations;  TEC irregularity oval},\r\ncorrespondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of SciencesRussian Federation; email: chernouss@pgia.ru},\r\npublisher={Pleiades Publishing},\r\nissn={19907931},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Phys. Chem. B},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A comparative analysis of total electron content (TEC) fluctuations and auroral activity, which characterizes the polar ionosphere during periods of substorm activity, is performed. The analysis is based on GPS/GLONASS observations at auroral and subauroral stations and data on auroral emissions obtained at the Yakutsk subauroral station and the Poker Flat (Alaska) auroral station. A detailed analysis is carried out for the storm on January 7, 2015. During this event, a sharp increase in the auroral activity and in TEC fluctuations from 09 to 13 UT was observed. A similarity of the dynamics of the auroral oval and the space–time distribution of TEC fluctuations associated with ionospheric irregularities in the oval is demonstrated. There is a close correlation between the positions of the auroral oval and the irregularity oval. The positions of the auroral oval, predicted by the NORUSKA Russian–Norwegian model, and of the TEC irregularity oval are compared. In general, the positions of the ovals are close to each other. The existing discrepancy may be due to the fact that the auroral oval is projected to an altitude of 110 km, whereas the irregularity oval, to an altitude of 450 km, and that the curvature of the magnetic force lines is not taken into account. The influence of auroral disturbances on navigational measurements manifests itself through the fact that, during disturbances, faults and failures in the operation of navigation equipment at auroral and subauroral stations increase. © 2018, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&amp;doi=10.1134%2fS1990519X18010030&amp;partnerID=40&amp;md5=f9de8e8533842e75b6479cbae6afc729\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&amp;doi=10.1134%2fS1990519X18010030&amp;partnerID=40&amp;md5=f9de8e8533842e75b6479cbae6afc729', event);\">\n    Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Burdakov, V.; Kovalev, R.; Pantina, R.; Varfolomeeva, E.; Makarov, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 12(1): 20-26.  2018.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&amp;doi=10.1134%2fS1990519X18010030&amp;partnerID=40&amp;md5=f9de8e8533842e75b6479cbae6afc729\"\n     onclick=\"log_download('burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&amp;doi=10.1134%2fS1990519X18010030&amp;partnerID=40&amp;md5=f9de8e8533842e75b6479cbae6afc729', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X18010030\"\n     onclick=\"log_download('burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018', 'http://doi.org/10.1134/S1990519X18010030', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('burdakov-kovalev-pantina-varfolomeeva-makarov-filatov-exosomestransferp53betweencellsandcansuppressgrowthandproliferationofp53negativecells-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Burdakov201820,\r\nauthor={Burdakov, V.S. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Filatov, M.V.},\r\ntitle={Exosomes Transfer p53 between Cells and Can Suppress Growth and Proliferation of p53-Negative Cells},\r\njournal={Cell and Tissue Biology},\r\nyear={2018},\r\nvolume={12},\r\nnumber={1},\r\npages={20-26},\r\ndoi={10.1134/S1990519X18010030},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042464427&amp;doi=10.1134%2fS1990519X18010030&amp;partnerID=40&amp;md5=f9de8e8533842e75b6479cbae6afc729},\r\naffiliation={Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 194064, Russian Federation; College of Health and Life Sciences, Brunel University, London, Uxbridge, United Kingdom; St. Petersburg Institute of Phthisiopulmonology, St. Petersburg, 191036, Russian Federation},\r\nabstract={Exosomes are nanosized vesicles that are secreted by many types of cells. We have found that exosomes secreted by HEK293 and HT-1080 can suppress growth and proliferation of p53-deficient cells. Upon overexpression of exogenous p53-GFP in HEK293 cells, we observed p53 protein in exosomes that were secreted by these cells. We also found endogenous p53 in exosomes that were secreted by HT-1080 cells with a higher level of p53 expression. We were able to detect endogenous p53 protein in exosomes that originated from human plasma and were transferred to p53-deficient cells. Our findings indicate that p53 protein can be transferred between cells and may play an important physiological role. © 2018, Pleiades Publishing, Ltd.},\r\nauthor_keywords={cell lines;  exosomes;  р53},\r\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre “Kurchatov Institute”Russian Federation; email: fil_53@mail.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exosomes are nanosized vesicles that are secreted by many types of cells. We have found that exosomes secreted by HEK293 and HT-1080 can suppress growth and proliferation of p53-deficient cells. Upon overexpression of exogenous p53-GFP in HEK293 cells, we observed p53 protein in exosomes that were secreted by these cells. We also found endogenous p53 in exosomes that were secreted by HT-1080 cells with a higher level of p53 expression. We were able to detect endogenous p53 protein in exosomes that originated from human plasma and were transferred to p53-deficient cells. Our findings indicate that p53 protein can be transferred between cells and may play an important physiological role. © 2018, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&amp;doi=10.1134%2fS199075081801002X&amp;partnerID=40&amp;md5=5c507aecac55632ff5640f00b0f7139c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&amp;doi=10.1134%2fS199075081801002X&amp;partnerID=40&amp;md5=5c507aecac55632ff5640f00b0f7139c', event);\">\n    Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Belyakova, N.; Pantina, R.; Kovalev, R.; Filatov, M.;  and Naryzhny, S.\n</span>\n\n  \n\n</span>\n\n  <i>Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry</i>, 12(1): 39-42.  2018.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&amp;doi=10.1134%2fS199075081801002X&amp;partnerID=40&amp;md5=5c507aecac55632ff5640f00b0f7139c\"\n     onclick=\"log_download('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&amp;doi=10.1134%2fS199075081801002X&amp;partnerID=40&amp;md5=5c507aecac55632ff5640f00b0f7139c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S199075081801002X\"\n     onclick=\"log_download('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018', 'http://doi.org/10.1134/S199075081801002X', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Belyakova201839,\r\nauthor={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},\r\ntitle={Quaternary Structures of Human Cytoplasmic and Nuclear PCNA Are the Same},\r\njournal={Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry},\r\nyear={2018},\r\nvolume={12},\r\nnumber={1},\r\npages={39-42},\r\ndoi={10.1134/S199075081801002X},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042775026&amp;doi=10.1134%2fS199075081801002X&amp;partnerID=40&amp;md5=5c507aecac55632ff5640f00b0f7139c},\r\naffiliation={Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov Institute, Leningrad region, Gatchina, 188300, Russian Federation},\r\nabstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-toback principle. Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well. © 2018, Pleiades Publishing, Ltd.},\r\nauthor_keywords={cytoplasm;  double trimer;  monomer;  neutrophil;  PCNA;  structure},\r\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute (PNPI), NRC Kurchatov InstituteRussian Federation; email: snaryzhny@mail.ru},\r\npublisher={Pleiades Publishing},\r\nissn={19907508},\r\nlanguage={English},\r\nabbrev_source_title={Biochem. (Moscow) Suppl. Ser. B Biomed. Chem.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-toback principle. Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well. © 2018, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&amp;doi=10.12688%2ff1000research.14406.1&amp;partnerID=40&amp;md5=5cdb17d6a9583e9bc8443ee0ec1012f7\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&amp;doi=10.12688%2ff1000research.14406.1&amp;partnerID=40&amp;md5=5cdb17d6a9583e9bc8443ee0ec1012f7', event);\">\n    Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Landa, S.; Korabliov, P.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>F1000Research</i>, 7.  2018.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&amp;doi=10.12688%2ff1000research.14406.1&amp;partnerID=40&amp;md5=5cdb17d6a9583e9bc8443ee0ec1012f7\"\n     onclick=\"log_download('filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&amp;doi=10.12688%2ff1000research.14406.1&amp;partnerID=40&amp;md5=5cdb17d6a9583e9bc8443ee0ec1012f7', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.12688/f1000research.14406.1\"\n     onclick=\"log_download('filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018', 'http://doi.org/10.12688/f1000research.14406.1', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-landa-korabliov-semenova-peculiaritiesoftheformationandsubsequentremovalofthecirculatingimmunecomplexesfromthebloodstreamduringtheprocessofdigestionversion1referees2approved1approvedwithreservations-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov2018,\r\nauthor={Filatov, M.V. and Landa, S.B. and Korabliov, P.V. and Semenova, E.V.},\r\ntitle={Peculiarities of the formation and subsequent removal of the circulating immune complexes from the bloodstream during the process of digestion [version 1; referees: 2 approved, 1 approved with reservations]},\r\njournal={F1000Research},\r\nyear={2018},\r\nvolume={7},\r\ndoi={10.12688/f1000research.14406.1},\r\nart_number={618},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050345456&amp;doi=10.12688%2ff1000research.14406.1&amp;partnerID=40&amp;md5=5cdb17d6a9583e9bc8443ee0ec1012f7},\r\naffiliation={Division of Molecular and Radiation Biophysics, National Research Center, Gatchina, 188300, Russian Federation; State Research Institute Center for Innovative Medicine, Vilnius, LT-01102, Lithuania; Saint Petersburg State Res Inst of Phthisiopulmonology of the Min of Healthcare of the Russian Fed, Saint Petersburg, 191036, Russian Federation},\r\nabstract={Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body&#x27;s immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7-8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular-cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely. © 2018 Landa SB et al.},\r\nauthor_keywords={Digestion;  Dynamic light scattering method;  irculating immune complexes;  Isotypes of immunoglobulins},\r\ncorrespondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, National Research CenterRussian Federation; email: fil_53@mail.ru},\r\npublisher={F1000 Research Ltd},\r\nissn={20461402},\r\nlanguage={English},\r\nabbrev_source_title={F1000 Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Background: Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids as a result of the development of the body's immune response to various provoking factors. The kinetic characteristics of the formation and removal of immune complexes (ICs), their physical parameters, the isotypic composition of immunoglobulins (Igs) and the antigenic component of the CICs may reflect certain aspects of certain pathological and metabolic processes taking place in humans and animals. The aim of this study is to assess the kinetic characteristics of the formation and removal of the CICs that form in blood after eating. We also analyze the changes in the isotypic composition of Igs of ICs that accompany this biological process in rodents and humans. Methods: We identified the CICs, which differed in size and class of Igs, using dynamic light scattering. To remove ICs from the plasma, we used immune-affinity sedimentation. Monoclonal antibodies for the Igs of different isotypes were added to the plasma samples to determine the isotypic composition of the ICs. Results: A large number of ICs were formed in the blood of rats and humans after eating (food CICs). In rats, food ICs are almost immediately filtered in the liver, without circulating in the bloodstream through the body. In humans, the level of food ICs in the blood increases for 3.5 h after ingestion, then within 7-8 h their gradual removal takes place. It was found that in the process of digestion in humans, the isotypic composition of Igs in the CICs changes and becomes more diverse. Conclusions: The molecular-cellular mechanisms of the formation and utilization of food CICs in humans and rodents do not match completely. © 2018 Landa SB et al.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&amp;doi=10.1139%2fbcb-2017-0277&amp;partnerID=40&amp;md5=78f514263ecc17ebbca9dc2f1d6906ac\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&amp;doi=10.1139%2fbcb-2017-0277&amp;partnerID=40&amp;md5=78f514263ecc17ebbca9dc2f1d6906ac', event);\">\n    Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Biochemistry and Cell Biology</i>, 96(4): 457-467.  2018.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&amp;doi=10.1139%2fbcb-2017-0277&amp;partnerID=40&amp;md5=78f514263ecc17ebbca9dc2f1d6906ac\"\n     onclick=\"log_download('anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051134186&amp;doi=10.1139%2fbcb-2017-0277&amp;partnerID=40&amp;md5=78f514263ecc17ebbca9dc2f1d6906ac', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1139/bcb-2017-0277\"\n     onclick=\"log_download('anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018', 'http://doi.org/10.1139/bcb-2017-0277', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-capacityofceruloplasmintoscavengeproductsoftherespiratoryburstofneutrophilsisnotalteredbytheproductsofreactionscatalyzedbymyeloperoxidase-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCl, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCl, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications. © 2018 Published by NRC Research Press.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&amp;doi=10.31116%2ftsitol.2018.07.05&amp;partnerID=40&amp;md5=4fb79a48075054ea3847204606cafb5e\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&amp;doi=10.31116%2ftsitol.2018.07.05&amp;partnerID=40&amp;md5=4fb79a48075054ea3847204606cafb5e', event);\">\n    In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Petrenko, E.; Kopylov, A.; Kleist, O.; Legina, O.; Belyakova, N.; Pantina, R.;  and Naryzhny, S.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 60(7): 519-523.  2018.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&amp;doi=10.31116%2ftsitol.2018.07.05&amp;partnerID=40&amp;md5=4fb79a48075054ea3847204606cafb5e\"\n     onclick=\"log_download('petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&amp;doi=10.31116%2ftsitol.2018.07.05&amp;partnerID=40&amp;md5=4fb79a48075054ea3847204606cafb5e', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.31116/tsitol.2018.07.05\"\n     onclick=\"log_download('petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018', 'http://doi.org/10.31116/tsitol.2018.07.05', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('petrenko-kopylov-kleist-legina-belyakova-pantina-naryzhny-insearchofspecificmarkersofglioblastomaanalysisofproteoformsofglioblastomacells-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Petrenko2018519,\r\nauthor={Petrenko, E.S. and Kopylov, A.T. and Kleist, O.A. and Legina, O.K. and Belyakova, N.V. and Pantina, R.A. and Naryzhny, S.N.},\r\ntitle={In search of specific markers of glioblastoma: Analysis of proteoforms of glioblastoma cells},\r\njournal={Tsitologiya},\r\nyear={2018},\r\nvolume={60},\r\nnumber={7},\r\npages={519-523},\r\ndoi={10.31116/tsitol.2018.07.05},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064618631&amp;doi=10.31116%2ftsitol.2018.07.05&amp;partnerID=40&amp;md5=4fb79a48075054ea3847204606cafb5e},\r\naffiliation={V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, 119121, Russian Federation; B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Centre Kurchatov Institute, Gatchina, Leningrad Region, 188300, Russian Federation},\r\nabstract={Proteins from normal (fibroblasts) and cancer (glioblastoma) human cells were separated by two dimensional gel electrophoresis. Next, a sectional analysis of these gels by mass spectrometry was performed, and profiles of proteoforms coded by more than 5000 genes were obtained. It was found that some proteins have specific for glioblastoma cells proteoforms. We assume that these proteoforms could be used as specific for glioblastoma biomarkers. © 2018 Sankt Peterburg. All rights reserved.},\r\nauthor_keywords={Biomarker;  Glioblastoma;  Mass spectrometry;  Proteoforma;  Proteome;  Two dimensional electrophoresis},\r\ncorrespondence_address1={Naryzhny, S.N.; V. N. Orekhovich Research Institute of Biomedical ChemistryRussian Federation; email: naryzhnyy_sn@pnpi.nrcki.ru},\r\npublisher={Sankt Peterburg},\r\nissn={00413771},\r\ncoden={TSITA},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Proteins from normal (fibroblasts) and cancer (glioblastoma) human cells were separated by two dimensional gel electrophoresis. Next, a sectional analysis of these gels by mass spectrometry was performed, and profiles of proteoforms coded by more than 5000 genes were obtained. It was found that some proteins have specific for glioblastoma cells proteoforms. We assume that these proteoforms could be used as specific for glioblastoma biomarkers. © 2018 Sankt Peterburg. All rights reserved.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&amp;doi=10.1134%2fS1990519X18010108&amp;partnerID=40&amp;md5=c3954c3ded80c915486fe0c5d05ab388\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&amp;doi=10.1134%2fS1990519X18010108&amp;partnerID=40&amp;md5=c3954c3ded80c915486fe0c5d05ab388', event);\">\n    Characterization of New Human Glioblastoma Cell Lines.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kiseleva, L.; Kartashev, A.; Vartanyan, N.; Pinevich, A.; Filatov, M.;  and Samoilovich, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 12(1).  2018.\n  <span class=\"note\">cited By 5</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&amp;doi=10.1134%2fS1990519X18010108&amp;partnerID=40&amp;md5=c3954c3ded80c915486fe0c5d05ab388\"\n     onclick=\"log_download('kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&amp;doi=10.1134%2fS1990519X18010108&amp;partnerID=40&amp;md5=c3954c3ded80c915486fe0c5d05ab388', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Characterization of New Human Glioblastoma Cell Lines [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X18010108\"\n     onclick=\"log_download('kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018', 'http://doi.org/10.1134/S1990519X18010108', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kiseleva-kartashev-vartanyan-pinevich-filatov-samoilovich-characterizationofnewhumanglioblastomacelllines-2018')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kiseleva2018,\r\nauthor={Kiseleva, L.N. and Kartashev, A.V. and Vartanyan, N.L. and Pinevich, A.A. and Filatov, M.V. and Samoilovich, M.P.},\r\ntitle={Characterization of New Human Glioblastoma Cell Lines},\r\njournal={Cell and Tissue Biology},\r\nyear={2018},\r\nvolume={12},\r\nnumber={1},\r\ndoi={10.1134/S1990519X18010108},\r\nnote={cited By 5},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042477662&amp;doi=10.1134%2fS1990519X18010108&amp;partnerID=40&amp;md5=c3954c3ded80c915486fe0c5d05ab388},\r\naffiliation={Russian Research Center for Radiology and Surgical Technologies, St. Petersburg, 197758, Russian Federation; St. Petersburg State University, St. Petersburg, 199034, Russian Federation; Northwestern State Medical University named after I.I. Mechnikov, St. Petersburg, 191015, Russian Federation; St. Petersburg Nuclear Physics Institute named after B.P. Konstantinov, St. Petersburg, 188300, Russian Federation},\r\nabstract={Glioblastomas are tumors of neuroectodermal origin with a high degree of diversity. Tumor cells isolated from the surgical material of patients with glioblastomas are heterogeneous populations varying in morphology, phenotype, and genetic characteristics. This paper presents a description of two new glioblastoma cell lines, R1 and T2, isolated from tumor tissue of patients in 2010. We investigated their morphological and cytochemical cell characteristics and the expression of neuronal, mesenchymal, and endothelial markers, as well as the activity of genes encoding a number of growth factors, extracellular matrix proteins, and intracellular proteins typical for cells of mesenchymal origin. R1 and T2 cell lines are morphologically different. T2 cell line was characterized by the presence of multinuclear cells. In terms of the expression of β-tubulin III, MGMT, and p53 protein, R1 cell line was more heterogeneous than T2. R1 and T2 glioblastoma cell lines also differed in the presence and ratio of cell populations with mesenchymal, neuronal, and endothelial markers. Thus, neuronal markers CD133/2 and CD56 were detected only on R1 cells. Both lines were characterized by high activity of growth factor genes TGFβ1, VEGF, and FGF2(b), lower activity of EGF, and high expression of THBS1 and αSMA genes. However, the activity of most of the genes under study in R1 cells was higher than in T2 cells. The greatest difference lay in the expression of HGF, FAP, and TNC. Comparison of two new glioblastoma cell lines, R1 and T2, with the continuously cultivated lines А172 and T98G showed that R1 line had remarkable similarity with A172, while T2 glioblastoma resembled T98G cells. Apparently, the differences between R1 and T2 cell lines are determined by the properties of the initial tumors rather than the time of the cell cultivation. © 2018, Pleiades Publishing, Ltd.},\r\nauthor_keywords={A172;  gene expression;  glioblastoma cell lines;  growth factors;  surface markers;  T98G},\r\ncorrespondence_address1={Kiseleva, L.N.; Russian Research Center for Radiology and Surgical TechnologiesRussian Federation; email: luba_kiseleva@mail.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Glioblastomas are tumors of neuroectodermal origin with a high degree of diversity. Tumor cells isolated from the surgical material of patients with glioblastomas are heterogeneous populations varying in morphology, phenotype, and genetic characteristics. This paper presents a description of two new glioblastoma cell lines, R1 and T2, isolated from tumor tissue of patients in 2010. We investigated their morphological and cytochemical cell characteristics and the expression of neuronal, mesenchymal, and endothelial markers, as well as the activity of genes encoding a number of growth factors, extracellular matrix proteins, and intracellular proteins typical for cells of mesenchymal origin. R1 and T2 cell lines are morphologically different. T2 cell line was characterized by the presence of multinuclear cells. In terms of the expression of β-tubulin III, MGMT, and p53 protein, R1 cell line was more heterogeneous than T2. R1 and T2 glioblastoma cell lines also differed in the presence and ratio of cell populations with mesenchymal, neuronal, and endothelial markers. Thus, neuronal markers CD133/2 and CD56 were detected only on R1 cells. Both lines were characterized by high activity of growth factor genes TGFβ1, VEGF, and FGF2(b), lower activity of EGF, and high expression of THBS1 and αSMA genes. However, the activity of most of the genes under study in R1 cells was higher than in T2 cells. The greatest difference lay in the expression of HGF, FAP, and TNC. Comparison of two new glioblastoma cell lines, R1 and T2, with the continuously cultivated lines А172 and T98G showed that R1 line had remarkable similarity with A172, while T2 glioblastoma resembled T98G cells. Apparently, the differences between R1 and T2 cell lines are determined by the properties of the initial tumors rather than the time of the cell cultivation. © 2018, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2017\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2017')\"\n      onkeypress=\"toggleGroup('group_2017')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2017</span>\n      <span class=\"bibbase_group_count\">\n        \n        (6)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&amp;doi=10.1063%2f1.5001645&amp;partnerID=40&amp;md5=d908b66243d4ade3a27c45bad492f886\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&amp;doi=10.1063%2f1.5001645&amp;partnerID=40&amp;md5=d908b66243d4ade3a27c45bad492f886', event);\">\n    Exosomes: Some approaches to cancer diagnosis and therapy.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Samsonov, R.; Kamyshinsky, R.; Pantina, R.; Verlov, N.; Vasiliev, A.; Konevega, A.;  and Malek, A.\n</span>\n\n  \n\n</span>\n\n   2017.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 5; Conference of International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference Date: 23 May 2017 Through 26 May 2017; Conference Code:133914</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&amp;doi=10.1063%2f1.5001645&amp;partnerID=40&amp;md5=d908b66243d4ade3a27c45bad492f886\"\n     onclick=\"log_download('shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&amp;doi=10.1063%2f1.5001645&amp;partnerID=40&amp;md5=d908b66243d4ade3a27c45bad492f886', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Exosomes: Some approaches to cancer diagnosis and therapy [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1063/1.5001645\"\n     onclick=\"log_download('shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017', 'http://doi.org/10.1063/1.5001645', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>1 download</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-samsonov-kamyshinsky-pantina-verlov-vasiliev-konevega-malek-exosomessomeapproachestocancerdiagnosisandtherapy-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Shtam2017,\r\nauthor={Shtam, T. and Samsonov, R. and Kamyshinsky, R. and Pantina, R. and Verlov, N. and Vasiliev, A. and Konevega, A.L. and Malek, A.V.},\r\ntitle={Exosomes: Some approaches to cancer diagnosis and therapy},\r\njournal={AIP Conference Proceedings},\r\nyear={2017},\r\nvolume={1882},\r\ndoi={10.1063/1.5001645},\r\nart_number={020066},\r\nnote={cited By 5; Conference of International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference Date: 23 May 2017 Through 26 May 2017;  Conference Code:133914},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041662414&amp;doi=10.1063%2f1.5001645&amp;partnerID=40&amp;md5=d908b66243d4ade3a27c45bad492f886},\r\naffiliation={Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov Institute, Gatchina, Russian Federation; Petrov Institute of Oncology, Saint-Petersburg, Russian Federation; Ltd. Oncosystem, Skolkovo, Russian Federation; Peter the Great Saint-Petersburg Polytechnic University, St. Petersburg, Russian Federation; National Research Center, Kurchatov Institute, Moscow, Russian Federation},\r\nabstract={Exosomes are membrane-bound, intercellular communication shuttle vesicles that are defined by their endocytic origin and size range of 30-120 nm. Secreted by nearly all mammalian cell types and present in bodily fluids, exosomes confer messages between cells, by transporting functionally relevant proteins, nucleic acids, and lipids. The capability of tumor exosomes to house tumorigenic information and induce cellular responses that promote disease pathogenesis make tumor exosomes an attractive tool in identifying cancer biomarkers and exploiting exosomes for therapy. In this paper, we sum up our previous findings to utilize exosomes as biomarkers for early detection, diagnosis and therapy selection of prostate and thyroid cancer and present our results on exosomes in colon cancer. Some of plasma exosomal miRNAs showed their potential as diagnostic markers for colon cancer. All together, the data suggested the potentials of circulating exosomal miRNAs as liquid biopsy markers for cancer. Here we also present the possibilities of delivering therapeutic molecules by exosomes. Previously, we had demonstrated the potential of exosome-mediated siRNA delivery. Here, we present the possibility of carrying the exogenous p53 protein by exosomes in vitro. © 2017 Author(s).},\r\ncorrespondence_address1={Shtam, T.; Petersburg Nuclear Physics Institute of National Research Centre, Kurchatov InstituteRussian Federation; email: tatyana_shtam@mail.ru},\r\neditor={Gutmanas E.Y., Naimark O.B., Sharkeev Y.P.},\r\nsponsors={Institute of Continuous Media Mechanics of UrB RAS; Institute of Strength Physics and Materials Science of SB RAS; National Research Tomsk Polytechnic University; National Research Tomsk State University; Tomsk Research Institute of Oncology},\r\npublisher={American Institute of Physics Inc.},\r\nissn={0094243X},\r\nisbn={9780735415621},\r\nlanguage={English},\r\nabbrev_source_title={AIP Conf. Proc.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exosomes are membrane-bound, intercellular communication shuttle vesicles that are defined by their endocytic origin and size range of 30-120 nm. Secreted by nearly all mammalian cell types and present in bodily fluids, exosomes confer messages between cells, by transporting functionally relevant proteins, nucleic acids, and lipids. The capability of tumor exosomes to house tumorigenic information and induce cellular responses that promote disease pathogenesis make tumor exosomes an attractive tool in identifying cancer biomarkers and exploiting exosomes for therapy. In this paper, we sum up our previous findings to utilize exosomes as biomarkers for early detection, diagnosis and therapy selection of prostate and thyroid cancer and present our results on exosomes in colon cancer. Some of plasma exosomal miRNAs showed their potential as diagnostic markers for colon cancer. All together, the data suggested the potentials of circulating exosomal miRNAs as liquid biopsy markers for cancer. Here we also present the possibilities of delivering therapeutic molecules by exosomes. Previously, we had demonstrated the potential of exosome-mediated siRNA delivery. Here, we present the possibility of carrying the exogenous p53 protein by exosomes in vitro. © 2017 Author(s).\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&amp;doi=10.1371%2fjournal.pone.0185126&amp;partnerID=40&amp;md5=e33272f5dbd1aae05eb33bbe72e9eea2\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&amp;doi=10.1371%2fjournal.pone.0185126&amp;partnerID=40&amp;md5=e33272f5dbd1aae05eb33bbe72e9eea2', event);\">\n    The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Makarov, E.; Shtam, T.; Kovalev, R.; Pantina, R.; Varfolomeeva, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>PLoS ONE</i>, 12(9).  2017.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&amp;doi=10.1371%2fjournal.pone.0185126&amp;partnerID=40&amp;md5=e33272f5dbd1aae05eb33bbe72e9eea2\"\n     onclick=\"log_download('makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&amp;doi=10.1371%2fjournal.pone.0185126&amp;partnerID=40&amp;md5=e33272f5dbd1aae05eb33bbe72e9eea2', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1371/journal.pone.0185126\"\n     onclick=\"log_download('makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017', 'http://doi.org/10.1371/journal.pone.0185126', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('makarov-shtam-kovalev-pantina-varfolomeeva-filatov-therarenonsensemutationinp53triggersalternativesplicingtoproduceaproteincapableofinducingapoptosis-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Makarov2017,\r\nauthor={Makarov, E.M. and Shtam, T.A. and Kovalev, R.A. and Pantina, R.A. and Varfolomeeva, E.Y. and Filatov, M.V.},\r\ntitle={The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis},\r\njournal={PLoS ONE},\r\nyear={2017},\r\nvolume={12},\r\nnumber={9},\r\ndoi={10.1371/journal.pone.0185126},\r\nart_number={e0185126},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030163652&amp;doi=10.1371%2fjournal.pone.0185126&amp;partnerID=40&amp;md5=e33272f5dbd1aae05eb33bbe72e9eea2},\r\naffiliation={Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; Peter the Great St Petersburg Polytechnic University, St Petersburg, Russian Federation; Petrov Institute of Oncology, St Petersburg, Russian Federation},\r\nabstract={P53 protein is more frequently mutated in human tumours compared with the other proteins. While the majority of the p53 mutations, especially within its DNA-binding domain, lead to the loss of the wild-type function, there are accumulating data demonstrating that the p53 mutants gain tumour promoting activities; the latter triggers a revitalised interest in functional analysis of the p53 mutants. A systematic screening for p53 mutations in surgical materials from patients with glioma revealed a 378C&gt;G mutation that creates a stop codon at the position of amino acid residue 126. The mutation eliminates the recognition site for the restriction endonuclease Sca I that allowed us to carry out RFLP analysis of DNA extracted from the clinical samples and suggests that this mutation is more frequent than is documented in the p53 databases. Both the ECV-304 and EJ cell lines, that probably originate from the bladder carcinoma T24 cell line, were confirmed to contain the homozygous 378C&gt;G mutation but were shown to produce the p53 protein of expected full-length size detected by Western blotting. We provide evidence that the 378C&gt;G mutation generates an alternative 3’ splice site (ss) which is more often used instead of the authentic upstream 3’ ss, driving the production of mRNA encoding the protein with the single amino acid deletion (p53ΔY126). Using endogenous expression, we demonstrated that the p53ΔY126 protein is nearly as active as the wild type protein in inducing the p21/Waf1 expression and apoptosis. © 2017 Makarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},\r\ncorrespondence_address1={Filatov, M.V.; National Research Centre Kurchatov Institute B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil53ster@gmail.com},\r\npublisher={Public Library of Science},\r\nissn={19326203},\r\ncoden={POLNC},\r\npubmed_id={28961258},\r\nlanguage={English},\r\nabbrev_source_title={PLoS ONE},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  P53 protein is more frequently mutated in human tumours compared with the other proteins. While the majority of the p53 mutations, especially within its DNA-binding domain, lead to the loss of the wild-type function, there are accumulating data demonstrating that the p53 mutants gain tumour promoting activities; the latter triggers a revitalised interest in functional analysis of the p53 mutants. A systematic screening for p53 mutations in surgical materials from patients with glioma revealed a 378C>G mutation that creates a stop codon at the position of amino acid residue 126. The mutation eliminates the recognition site for the restriction endonuclease Sca I that allowed us to carry out RFLP analysis of DNA extracted from the clinical samples and suggests that this mutation is more frequent than is documented in the p53 databases. Both the ECV-304 and EJ cell lines, that probably originate from the bladder carcinoma T24 cell line, were confirmed to contain the homozygous 378C>G mutation but were shown to produce the p53 protein of expected full-length size detected by Western blotting. We provide evidence that the 378C>G mutation generates an alternative 3’ splice site (ss) which is more often used instead of the authentic upstream 3’ ss, driving the production of mRNA encoding the protein with the single amino acid deletion (p53ΔY126). Using endogenous expression, we demonstrated that the p53ΔY126 protein is nearly as active as the wild type protein in inducing the p21/Waf1 expression and apoptosis. © 2017 Makarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&amp;doi=10.1103%2fPhysRevE.96.012411&amp;partnerID=40&amp;md5=e15ead8307ee2efbb140f35ce40ca971\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&amp;doi=10.1103%2fPhysRevE.96.012411&amp;partnerID=40&amp;md5=e15ead8307ee2efbb140f35ce40ca971', event);\">\n    Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Iashina, E.; Velichko, E.; Filatov, M.; Bouwman, W.; Duif, C.; Brulet, A.;  and Grigoriev, S.\n</span>\n\n  \n\n</span>\n\n  <i>Physical Review E</i>, 96(1).  2017.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&amp;doi=10.1103%2fPhysRevE.96.012411&amp;partnerID=40&amp;md5=e15ead8307ee2efbb140f35ce40ca971\"\n     onclick=\"log_download('iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&amp;doi=10.1103%2fPhysRevE.96.012411&amp;partnerID=40&amp;md5=e15ead8307ee2efbb140f35ce40ca971', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1103/PhysRevE.96.012411\"\n     onclick=\"log_download('iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017', 'http://doi.org/10.1103/PhysRevE.96.012411', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('iashina-velichko-filatov-bouwman-duif-brulet-grigoriev-additivescalinglawforstructuralorganizationofchromatininchickenerythrocytenuclei-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Iashina2017,\r\nauthor={Iashina, E.G. and Velichko, E.V. and Filatov, M.V. and Bouwman, W.G. and Duif, C.P. and Brulet, A. and Grigoriev, S.V.},\r\ntitle={Additive scaling law for structural organization of chromatin in chicken erythrocyte nuclei},\r\njournal={Physical Review E},\r\nyear={2017},\r\nvolume={96},\r\nnumber={1},\r\ndoi={10.1103/PhysRevE.96.012411},\r\nart_number={012411},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026472847&amp;doi=10.1103%2fPhysRevE.96.012411&amp;partnerID=40&amp;md5=e15ead8307ee2efbb140f35ce40ca971},\r\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, St.-Petersburg, 188300, Russian Federation; Saint Petersburg State University, Ulyanovskaya 1, St.-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, JB Delft, 2629, Netherlands; Leon Brillouin Laboratory, CEA Saclay, Gif sur Yvette Cedex, 91191, France},\r\nabstract={Small-angle neutron scattering (SANS) on nuclei of chicken erythrocytes demonstrates the cubic dependence of the scattering intensity Q-3 in the range of momentum transfer Q 10-3-10-2nm-1. Independent spin-echo SANS measurements give the spin-echo function, which is well described by the exponential law in a range of sizes (3×102)-(3×104) nm. Both experimental dependences reflect the nature of the structural organization of chromatin in the nucleus of a living cell, which corresponds to the correlation function γ(r)=ln(ξ/r) for r&lt;ξ, where ξ=(3.69±0.07)×103 nm, the size of the nucleus. It has the specific scaling property of the logarithmic fractal γ(r/a)=γ(r)+ln(a), i.e., the scaling down by a gives an additive constant to the correlation function, which distinguishes it from the mass fractal, which is characterized by multiplicative constant. © 2017 American Physical Society.},\r\npublisher={American Physical Society},\r\nissn={24700045},\r\npubmed_id={29347273},\r\nlanguage={English},\r\nabbrev_source_title={Phys. Rev. E},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Small-angle neutron scattering (SANS) on nuclei of chicken erythrocytes demonstrates the cubic dependence of the scattering intensity Q-3 in the range of momentum transfer Q 10-3-10-2nm-1. Independent spin-echo SANS measurements give the spin-echo function, which is well described by the exponential law in a range of sizes (3×102)-(3×104) nm. Both experimental dependences reflect the nature of the structural organization of chromatin in the nucleus of a living cell, which corresponds to the correlation function γ(r)=ln(ξ/r) for r<ξ, where ξ=(3.69±0.07)×103 nm, the size of the nucleus. It has the specific scaling property of the logarithmic fractal γ(r/a)=γ(r)+ln(a), i.e., the scaling down by a gives an additive constant to the correlation function, which distinguishes it from the mass fractal, which is characterized by multiplicative constant. © 2017 American Physical Society.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&amp;doi=10.1088%2f1742-6596%2f862%2f1%2f012010&amp;partnerID=40&amp;md5=33d0632e7d097bc7f05412a5d0883c38\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&amp;doi=10.1088%2f1742-6596%2f862%2f1%2f012010&amp;partnerID=40&amp;md5=33d0632e7d097bc7f05412a5d0883c38', event);\">\n    Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Iashina, E.; Bouwman, W.; Duif, C.; Filatov, M.;  and Grigoriev, S.\n</span>\n\n  \n\n</span>\n\n   2017.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 0; Conference of 11th International Conference on Polarised Neutrons for Condensed Matter Investigations, PNCMI 2016 ; Conference Date: 4 July 2016 Through 7 July 2016; Conference Code:128574</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&amp;doi=10.1088%2f1742-6596%2f862%2f1%2f012010&amp;partnerID=40&amp;md5=33d0632e7d097bc7f05412a5d0883c38\"\n     onclick=\"log_download('iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&amp;doi=10.1088%2f1742-6596%2f862%2f1%2f012010&amp;partnerID=40&amp;md5=33d0632e7d097bc7f05412a5d0883c38', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1088/1742-6596/862/1/012010\"\n     onclick=\"log_download('iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017', 'http://doi.org/10.1088/1742-6596/862/1/012010', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('iashina-bouwman-duif-filatov-grigoriev-spinechosmallangleneutronscatteringstudyofthestructureorganizationofthechromatininbiologicalcell-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Iashina2017,\r\nauthor={Iashina, E.G. and Bouwman, W.G. and Duif, C.P. and Filatov, M.V. and Grigoriev, S.V.},\r\ntitle={Spin-echo small-angle neutron scattering study of the structure organization of the chromatin in biological cell},\r\njournal={Journal of Physics: Conference Series},\r\nyear={2017},\r\nvolume={862},\r\nnumber={1},\r\ndoi={10.1088/1742-6596/862/1/012010},\r\nart_number={012010},\r\nnote={cited By 0; Conference of 11th International Conference on Polarised Neutrons for Condensed Matter Investigations, PNCMI 2016 ; Conference Date: 4 July 2016 Through 7 July 2016;  Conference Code:128574},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040723666&amp;doi=10.1088%2f1742-6596%2f862%2f1%2f012010&amp;partnerID=40&amp;md5=33d0632e7d097bc7f05412a5d0883c38},\r\naffiliation={Petersburg Nuclear Physics Institute NRC KI, Gatchina, St-Petersburg, 188300, Russian Federation; Saint-Petersburg State University, Saint-Petersburg, 198504, Russian Federation; Delft University of Technology, Mekelweg 15, Delft, 2629 JB, Netherlands; Department of Physics, University of Bristol, Tyndalls Park Road, Bristol, BS8 1TS, United Kingdom},\r\nabstract={Spin-echo small-angle scattering (SESANS) technique is a method to measure the structure of materials from nano- to micrmeter length scales. This method could be important for studying the packaging of DNA in the eukaryotic cell. We measured the SESANS function from chicken erythrocyte nuclei which is well fitted by the exponential function G(z) = exp(-z/ξ), where ξ is the correlation length of a nucleus (in experimental data ξ = 3, 3 μm). The exponential decay of G(z) corresponds to the logarithmic pair correlation function γ(r) = ln(ξ/r). As the sensitivity of the SESANS signal depends on the neutron wavelength, we propose the SESANS setup with the changeable wavelength in the range from 2 to 12 Å. Such option allows one to study in great detail the internal structure of the biological cell in the length scale from 10-2 μm to 10 μm. © Published under licence by IOP Publishing Ltd.},\r\nsponsors={AIRBUS DS; et al.; ISIS; J-PARC; LLB; Swiss Neutronics},\r\npublisher={Institute of Physics Publishing},\r\nissn={17426588},\r\nlanguage={English},\r\nabbrev_source_title={J. Phys. Conf. Ser.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Spin-echo small-angle scattering (SESANS) technique is a method to measure the structure of materials from nano- to micrmeter length scales. This method could be important for studying the packaging of DNA in the eukaryotic cell. We measured the SESANS function from chicken erythrocyte nuclei which is well fitted by the exponential function G(z) = exp(-z/ξ), where ξ is the correlation length of a nucleus (in experimental data ξ = 3, 3 μm). The exponential decay of G(z) corresponds to the logarithmic pair correlation function γ(r) = ln(ξ/r). As the sensitivity of the SESANS signal depends on the neutron wavelength, we propose the SESANS setup with the changeable wavelength in the range from 2 to 12 Å. Such option allows one to study in great detail the internal structure of the biological cell in the length scale from 10-2 μm to 10 μm. © Published under licence by IOP Publishing Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&amp;doi=10.1134%2fS1990519X17020043&amp;partnerID=40&amp;md5=f439cee2262ea4552aa9c299648a59e1\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&amp;doi=10.1134%2fS1990519X17020043&amp;partnerID=40&amp;md5=f439cee2262ea4552aa9c299648a59e1', event);\">\n    Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Burdakov, V.; Landa, S.; Naryzhny, S.; Bairamukov, V.; Malek, A.; Orlov, Y.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 11(2): 172-179.  2017.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&amp;doi=10.1134%2fS1990519X17020043&amp;partnerID=40&amp;md5=f439cee2262ea4552aa9c299648a59e1\"\n     onclick=\"log_download('shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&amp;doi=10.1134%2fS1990519X17020043&amp;partnerID=40&amp;md5=f439cee2262ea4552aa9c299648a59e1', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X17020043\"\n     onclick=\"log_download('shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017', 'http://doi.org/10.1134/S1990519X17020043', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-burdakov-landa-naryzhny-bairamukov-malek-orlov-filatov-aggregationbylectinsasanapproachforexosomeisolationfrombiologicalfluidsvalidationforproteomicstudies-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2017172,\r\nauthor={Shtam, T.A. and Burdakov, V.S. and Landa, S.B. and Naryzhny, S.N. and Bairamukov, V.Y. and Malek, A.V. and Orlov, Y.N. and Filatov, M.V.},\r\ntitle={Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies},\r\njournal={Cell and Tissue Biology},\r\nyear={2017},\r\nvolume={11},\r\nnumber={2},\r\npages={172-179},\r\ndoi={10.1134/S1990519X17020043},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&amp;doi=10.1134%2fS1990519X17020043&amp;partnerID=40&amp;md5=f439cee2262ea4552aa9c299648a59e1},\r\naffiliation={National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Peter the Great Polytechnic University, St. Petersburg, 195251, Russian Federation; Petrov Institute of Oncology, Ministry of Healthcare of the Russian Federation, St. Petersburg, 197758, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russian Federation},\r\nabstract={Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.},\r\nauthor_keywords={exosomes;  lectins;  methods of exosome isolation},\r\ncorrespondence_address1={Filatov, M.V.; National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil_53@mail.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&amp;doi=10.18097%2fPBMC20176304356&amp;partnerID=40&amp;md5=ce5cadb78677efafb3f40f9a9cf8706b\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&amp;doi=10.18097%2fPBMC20176304356&amp;partnerID=40&amp;md5=ce5cadb78677efafb3f40f9a9cf8706b', event);\">\n    Quaternary structures of human cytoplasmic and nuclear PCNA are the same.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Belyakova, N.; Pantina, R.; Kovalev, R.; Filatov, M.;  and Naryzhny, S.\n</span>\n\n  \n\n</span>\n\n  <i>Biomeditsinskaya Khimiya</i>, 63(4): 356-360.  2017.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&amp;doi=10.18097%2fPBMC20176304356&amp;partnerID=40&amp;md5=ce5cadb78677efafb3f40f9a9cf8706b\"\n     onclick=\"log_download('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&amp;doi=10.18097%2fPBMC20176304356&amp;partnerID=40&amp;md5=ce5cadb78677efafb3f40f9a9cf8706b', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Quaternary structures of human cytoplasmic and nuclear PCNA are the same [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.18097/PBMC20176304356\"\n     onclick=\"log_download('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017', 'http://doi.org/10.18097/PBMC20176304356', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('belyakova-pantina-kovalev-filatov-naryzhny-quaternarystructuresofhumancytoplasmicandnuclearpcnaarethesame-2017')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Belyakova2017356,\r\nauthor={Belyakova, N.V. and Pantina, R.A. and Kovalev, R.A. and Filatov, M.V. and Naryzhny, S.N.},\r\ntitle={Quaternary structures of human cytoplasmic and nuclear PCNA are the same},\r\njournal={Biomeditsinskaya Khimiya},\r\nyear={2017},\r\nvolume={63},\r\nnumber={4},\r\npages={356-360},\r\ndoi={10.18097/PBMC20176304356},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028754223&amp;doi=10.18097%2fPBMC20176304356&amp;partnerID=40&amp;md5=ce5cadb78677efafb3f40f9a9cf8706b},\r\naffiliation={Petersburg Nuclear Physics Institute NRC Kurchatov Institute, Leningrad Region (PNPI), Gatchina, 188300, Russian Federation},\r\nabstract={Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-to-back principle (Naryzhny S.N. et al. (2005) J. Biol. Chem., 280, 13888). Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well.},\r\nauthor_keywords={Cytoplasm;  Double trimer;  Monomer;  Neutrophil;  PCNA;  Structure},\r\npublisher={Russian Academy of Medical Sciences},\r\nissn={23106905},\r\npubmed_id={28862608},\r\nlanguage={Russian},\r\nabbrev_source_title={Biomeditsinskaya Khim.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Properties and mechanisms of PCNA (proliferating cell nuclear antigen) functions have been investigated for a long time and are studied in great detail. As follows from its name, most known PCNA functions (DNA replication, DNA repair, DNA recombination and others) are connected with cell proliferation and localization of this protein in nuclei. In addition, there is good reason to believe that PCNA also performs some functions in the cytoplasm. However, the possible role and mechanisms of PCNA action in the cytoplasm require careful study and clarification. Interestingly, such cells as neutrophils differ in that they are non-dividing on one hand and on the other hand contain a rather large amount of PCNA, which is localized only in the cytoplasm, that is, they are an ideal model for the study of cytoplasmic PCNA. Using cross-linkages with formaldehyde, we showed that this cytoplasmic PCNA is cross-linked in a similar way, that is, organized in the same way as the nuclear PCNA that is present in the proliferating cells. Previously, we showed that PCNA in such cells is organized into a dynamic complex of double trimer on the basis of the back-to-back principle (Naryzhny S.N. et al. (2005) J. Biol. Chem., 280, 13888). Apparently, such organization of this hub-protein allows it to better coordinate the processes taking place in the cytoplasm as well.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2016\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2016')\"\n      onkeypress=\"toggleGroup('group_2016')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2016</span>\n      <span class=\"bibbase_group_count\">\n        \n        (5)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&amp;doi=10.1007%2fs13277-016-5065-3&amp;partnerID=40&amp;md5=44202226962197cb45fd5d86662beabe\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&amp;doi=10.1007%2fs13277-016-5065-3&amp;partnerID=40&amp;md5=44202226962197cb45fd5d86662beabe', event);\">\n    Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Samsonov, R.; Burdakov, V.; Shtam, T.; Radzhabovа, Z.; Vasilyev, D.; Tsyrlina, E.; Titov, S.; Ivanov, M.; Berstein, L.; Filatov, M.; Kolesnikov, N.; Gil-Henn, H.;  and Malek, A.\n</span>\n\n  \n\n</span>\n\n  <i>Tumor Biology</i>, 37(9): 12011-12021.  2016.\n  <span class=\"note\">cited By 29</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&amp;doi=10.1007%2fs13277-016-5065-3&amp;partnerID=40&amp;md5=44202226962197cb45fd5d86662beabe\"\n     onclick=\"log_download('samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&amp;doi=10.1007%2fs13277-016-5065-3&amp;partnerID=40&amp;md5=44202226962197cb45fd5d86662beabe', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/s13277-016-5065-3\"\n     onclick=\"log_download('samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016', 'http://doi.org/10.1007/s13277-016-5065-3', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('samsonov-burdakov-shtam-radzhabov-vasilyev-tsyrlina-titov-ivanov-etal-plasmaexosomalmir21andmir181adifferentiatesfollicularfrompapillarythyroidcancer-2016')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Samsonov201612011,\r\nauthor={Samsonov, R. and Burdakov, V. and Shtam, T. and Radzhabovа, Z. and Vasilyev, D. and Tsyrlina, E. and Titov, S. and Ivanov, M. and Berstein, L. and Filatov, M. and Kolesnikov, N. and Gil-Henn, H. and Malek, A.},\r\ntitle={Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer},\r\njournal={Tumor Biology},\r\nyear={2016},\r\nvolume={37},\r\nnumber={9},\r\npages={12011-12021},\r\ndoi={10.1007/s13277-016-5065-3},\r\nnote={cited By 29},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966489917&amp;doi=10.1007%2fs13277-016-5065-3&amp;partnerID=40&amp;md5=44202226962197cb45fd5d86662beabe},\r\naffiliation={Oncosystem Ltd, Hoshimina 11/1-207, Saint-Petersburg, 194356, Russian Federation; NN Petrov Institute of Oncology, Leningradskaya 68, Saint-Petersburg, 197758, Russian Federation; FSBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg  188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, 195251, Russian Federation; Institute of Molecular and Cellular Biology SB RAS, Lavrentieva 8/2, Novosibirsk, 630090, Russian Federation; Faculty of Medicine in the Galilee, Bar-Ilan University, Henrietta Szold 8, Safed, 13100, Israel},\r\nabstract={Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over the last few decades. As has been revealed by a number of studies, TC tissue’s micro-RNA (miRNA) profile may reflect histological features and the clinical behavior of tumor. However, alteration of the miRNA profile of plasma exosomes associated with TC development has to date not been explored. We isolated exosomes from plasma and assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, and western blotting. Next, we profiled cancer-associated miRNAs in plasma exosomes obtained from papillary TC patients, before and after surgical removal of the tumor. The diagnostic value of selected miRNAs was evaluated in a large cohort of patients displaying different statuses of thyroid nodule disease. MiRNA assessment was performed by RT-qPCR. In total, 60 patients with different types of thyroid nodal pathology were included in the study. Our results revealed that the development of papillary TC is associated with specific changes in exosomal miRNA profiles; this phenomenon can be used for differential diagnostics. MiRNA-31 was found to be over-represented in the plasma exosomes of patients with papillary TC vs. benign tumors, while miRNA-21 helped to distinguish between benign tumors and follicular TC. MiRNA-21 and MiRNA-181a-5p were found to be expressed reciprocally in the exosomes of patients with papillary and follicular TC, and their comparative assessment may help to distinguish between these types of TC with 100 % sensitivity and 77 % specificity. © 2016, International Society of Oncology and BioMarkers (ISOBM).},\r\nauthor_keywords={Diagnostics;  Exosomes;  MicroRNA;  Thyroid cancer},\r\ncorrespondence_address1={Malek, A.; Oncosystem Ltd, Hoshimina 11/1-207, Russian Federation; email: anastasia@malek.com},\r\npublisher={Springer Netherlands},\r\nissn={10104283},\r\ncoden={TUMBE},\r\npubmed_id={27164936},\r\nlanguage={English},\r\nabbrev_source_title={Tumor Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over the last few decades. As has been revealed by a number of studies, TC tissue’s micro-RNA (miRNA) profile may reflect histological features and the clinical behavior of tumor. However, alteration of the miRNA profile of plasma exosomes associated with TC development has to date not been explored. We isolated exosomes from plasma and assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, and western blotting. Next, we profiled cancer-associated miRNAs in plasma exosomes obtained from papillary TC patients, before and after surgical removal of the tumor. The diagnostic value of selected miRNAs was evaluated in a large cohort of patients displaying different statuses of thyroid nodule disease. MiRNA assessment was performed by RT-qPCR. In total, 60 patients with different types of thyroid nodal pathology were included in the study. Our results revealed that the development of papillary TC is associated with specific changes in exosomal miRNA profiles; this phenomenon can be used for differential diagnostics. MiRNA-31 was found to be over-represented in the plasma exosomes of patients with papillary TC vs. benign tumors, while miRNA-21 helped to distinguish between benign tumors and follicular TC. MiRNA-21 and MiRNA-181a-5p were found to be expressed reciprocally in the exosomes of patients with papillary and follicular TC, and their comparative assessment may help to distinguish between these types of TC with 100 % sensitivity and 77 % specificity. © 2016, International Society of Oncology and BioMarkers (ISOBM).\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&amp;doi=10.1080%2f10715762.2016.1197395&amp;partnerID=40&amp;md5=e0176bf5319caea3ccbca3344954e1b4\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&amp;doi=10.1080%2f10715762.2016.1197395&amp;partnerID=40&amp;md5=e0176bf5319caea3ccbca3344954e1b4', event);\">\n    Ceruloplasmin decreases respiratory burst reaction during pregnancy.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Varfolomeeva, E.; Semenova, E.; Sokolov, A.; Aplin, K.; Timofeeva, K.; Vasilyev, V.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Free Radical Research</i>, 50(8): 909-919.  2016.\n  <span class=\"note\">cited By 8</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&amp;doi=10.1080%2f10715762.2016.1197395&amp;partnerID=40&amp;md5=e0176bf5319caea3ccbca3344954e1b4\"\n     onclick=\"log_download('varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&amp;doi=10.1080%2f10715762.2016.1197395&amp;partnerID=40&amp;md5=e0176bf5319caea3ccbca3344954e1b4', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Ceruloplasmin decreases respiratory burst reaction during pregnancy [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1080/10715762.2016.1197395\"\n     onclick=\"log_download('varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016', 'http://doi.org/10.1080/10715762.2016.1197395', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('varfolomeeva-semenova-sokolov-aplin-timofeeva-vasilyev-filatov-ceruloplasmindecreasesrespiratoryburstreactionduringpregnancy-2016')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Varfolomeeva2016909,\r\nauthor={Varfolomeeva, E.Y. and Semenova, E.V. and Sokolov, A.V. and Aplin, K.D. and Timofeeva, K.E. and Vasilyev, V.B. and Filatov, M.V.},\r\ntitle={Ceruloplasmin decreases respiratory burst reaction during pregnancy},\r\njournal={Free Radical Research},\r\nyear={2016},\r\nvolume={50},\r\nnumber={8},\r\npages={909-919},\r\ndoi={10.1080/10715762.2016.1197395},\r\nnote={cited By 8},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978608243&amp;doi=10.1080%2f10715762.2016.1197395&amp;partnerID=40&amp;md5=e0176bf5319caea3ccbca3344954e1b4},\r\naffiliation={Division of Molecular and Radiation Biophysics, SFBI “Petersburg Nuclear Physics Institute” (National Research Center “Kurchatov Institute”), Gatchina, Russian Federation; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, St. Petersburg, Russian Federation; Chair of Fundamental Problems of Medicine, Saint-Petersburg State University, St. Petersburg, Russian Federation},\r\nabstract={Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin – a copper-containing glycoprotein – is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy. © 2016 Informa UK Limited, trading as Taylor &amp; Francis Group.},\r\nauthor_keywords={Ceruloplasmin;  flow cytometry;  neutrophils;  pregnancy;  respiratory burst reaction},\r\ncorrespondence_address1={Sokolov, A.V.; Department of Molecular Genetics, FSBSI “Institute of Experimental Medicine”, Acad. Pavlova str., 12, Russian Federation; email: biochemsokolov@gmail.com},\r\npublisher={Taylor and Francis Ltd},\r\nissn={10715762},\r\ncoden={FRARE},\r\npubmed_id={27266720},\r\nlanguage={English},\r\nabbrev_source_title={Free Radic. Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin – a copper-containing glycoprotein – is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy. © 2016 Informa UK Limited, trading as Taylor & Francis Group.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&amp;doi=10.1134%2fS1990519X16040118&amp;partnerID=40&amp;md5=9aafccdeff858ecc1fc8db1b00051926\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&amp;doi=10.1134%2fS1990519X16040118&amp;partnerID=40&amp;md5=9aafccdeff858ecc1fc8db1b00051926', event);\">\n    Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Volnitsky, A.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 10(4): 259-263.  2016.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&amp;doi=10.1134%2fS1990519X16040118&amp;partnerID=40&amp;md5=9aafccdeff858ecc1fc8db1b00051926\"\n     onclick=\"log_download('semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&amp;doi=10.1134%2fS1990519X16040118&amp;partnerID=40&amp;md5=9aafccdeff858ecc1fc8db1b00051926', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X16040118\"\n     onclick=\"log_download('semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016', 'http://doi.org/10.1134/S1990519X16040118', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-volnitsky-filatov-assayoflgi1geneepigeneticalterationsbyposttranslationalmodificationsofh3histoneinmalignantgliomas-2016')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2016259,\r\nauthor={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},\r\ntitle={Assay of LGI1 gene epigenetic alterations by posttranslational modifications of H3 histone in malignant gliomas},\r\njournal={Cell and Tissue Biology},\r\nyear={2016},\r\nvolume={10},\r\nnumber={4},\r\npages={259-263},\r\ndoi={10.1134/S1990519X16040118},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981727023&amp;doi=10.1134%2fS1990519X16040118&amp;partnerID=40&amp;md5=9aafccdeff858ecc1fc8db1b00051926},\r\naffiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov Institute, Gatchina, 188300, Russian Federation},\r\nabstract={Although progress has been made in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that glioma malignant transformation is accompanied by gradual loss of LGI1 gene expression. However, genetic defects underlying LGI1 inactivation are not recognized. In this work, we analyzed LGI1 gene expression in primary cultures of malignant gliomas and compared these data with posttranslational modifications of H3 histone, an epigenetic indicator of transcriptional activity. We showed the presence of an epigenetic marker of gene repression H3K9me3 near LGL1 transcription initiation site in most malignant gliomas (five out of six). No expression of LGI1 gene was registered in these gliomas. LGI1 expression was documented only in one glioma, and this tumor did not exhibit an association of LGI1 gene with H3K9me3 modification. Thus, we demonstrated for the first time a correlation between an LGI1 gene expression and epigenetic indicator H3K9met3 in malignant gliomas. An Н3К4ас marker of actively transcribed chromatin near LGI1 transcription initiation site was not found. These data suggest the inactivation of LGI1 gene through an epigenetic mechanism: a modified “histone code.” © 2016, Pleiades Publishing, Ltd.},\r\nauthor_keywords={epigenetic regulation;  gliomas;  histone code;  LGI1 gene},\r\ncorrespondence_address1={Semenova, E.V.; Konstantinov St. Petersburg Nuclear Physics Institute, Russian Research Centre Kurchatov InstituteRussian Federation; email: semenova_el.spb@mail.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Although progress has been made in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that glioma malignant transformation is accompanied by gradual loss of LGI1 gene expression. However, genetic defects underlying LGI1 inactivation are not recognized. In this work, we analyzed LGI1 gene expression in primary cultures of malignant gliomas and compared these data with posttranslational modifications of H3 histone, an epigenetic indicator of transcriptional activity. We showed the presence of an epigenetic marker of gene repression H3K9me3 near LGL1 transcription initiation site in most malignant gliomas (five out of six). No expression of LGI1 gene was registered in these gliomas. LGI1 expression was documented only in one glioma, and this tumor did not exhibit an association of LGI1 gene with H3K9me3 modification. Thus, we demonstrated for the first time a correlation between an LGI1 gene expression and epigenetic indicator H3K9met3 in malignant gliomas. An Н3К4ас marker of actively transcribed chromatin near LGI1 transcription initiation site was not found. These data suggest the inactivation of LGI1 gene through an epigenetic mechanism: a modified “histone code.” © 2016, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&amp;partnerID=40&amp;md5=9d291f5873458153cdec69d5434ac722\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&amp;partnerID=40&amp;md5=9d291f5873458153cdec69d5434ac722', event);\">\n    THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Volnitsky, A.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiia</i>, 58(5): 335-339.  2016.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&amp;partnerID=40&amp;md5=9d291f5873458153cdec69d5434ac722\"\n     onclick=\"log_download('semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&amp;partnerID=40&amp;md5=9d291f5873458153cdec69d5434ac722', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-volnitsky-filatov-theanalysisoflgi1geneepigeneticalterationsbymeansofposttranslationalh3histonemodificationsinmalignantgliomas-2016')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2016335,\r\nauthor={Semenova, E.V. and Volnitsky, A.V. and Filatov, M.V.},\r\ntitle={THE ANALYSIS OF LGI1 GENE EPIGENETIC ALTERATIONS BY MEANS OF POSTTRANSLATIONAL H3 HISTONE MODIFICATIONS IN MALIGNANT GLIOMAS},\r\njournal={Tsitologiia},\r\nyear={2016},\r\nvolume={58},\r\nnumber={5},\r\npages={335-339},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053720310&amp;partnerID=40&amp;md5=9d291f5873458153cdec69d5434ac722},\r\nabstract={Although there is a progress in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that process of malignant transformation of gliomas is accompanied by gradual loss of LGI1 gene expression. However, genetic defects causing LGI1 inactivation have not been revealed. In this paper, we have analyzed the LGI1 gene expression in primary cultures of malignant gliomas, and compared these data with epigenetic indicators of transcriptional activity — posttranslational H3 histone modifications. We have show the presence of an epigenetic marker of gene repression H3K9me3 near the site of LGI1 transcription initiation in most (5 from 6) studied gliomas. There was not LGI1 expression in these gliomas. Only one glioma showed LGI1 expression, and in this glioma there was no association of LGI1gene with H3K9me3 modification. Thus, we are the first to show a correlation between LGI1 gene expression and the epigenetic indicator H3K9met3 in malignant gliomas. Marker of actively transcribed chromatin Í3K4àñ have not been found in this area of the genome. The data obtained strongly suggest the possibility of gene LGL1 inactivation by epigenetic mechanism: modified «histone code».},\r\nissn={00413771},\r\npubmed_id={30188624},\r\nlanguage={English; Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Although there is a progress in understanding the causes and consequences of genetic and epigenetic changes in glioma malignant transformation, many details remain obscure and need further investigation. It is known that process of malignant transformation of gliomas is accompanied by gradual loss of LGI1 gene expression. However, genetic defects causing LGI1 inactivation have not been revealed. In this paper, we have analyzed the LGI1 gene expression in primary cultures of malignant gliomas, and compared these data with epigenetic indicators of transcriptional activity — posttranslational H3 histone modifications. We have show the presence of an epigenetic marker of gene repression H3K9me3 near the site of LGI1 transcription initiation in most (5 from 6) studied gliomas. There was not LGI1 expression in these gliomas. Only one glioma showed LGI1 expression, and in this glioma there was no association of LGI1gene with H3K9me3 modification. Thus, we are the first to show a correlation between LGI1 gene expression and the epigenetic indicator H3K9met3 in malignant gliomas. Marker of actively transcribed chromatin Í3K4àñ have not been found in this area of the genome. The data obtained strongly suggest the possibility of gene LGL1 inactivation by epigenetic mechanism: modified «histone code».\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&amp;doi=10.1002%2fpros.23101&amp;partnerID=40&amp;md5=10835f69427cb4f1655bac5857581ea3\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&amp;doi=10.1002%2fpros.23101&amp;partnerID=40&amp;md5=10835f69427cb4f1655bac5857581ea3', event);\">\n    Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Samsonov, R.; Shtam, T.; Burdakov, V.; Glotov, A.; Tsyrlina, E.; Berstein, L.; Nosov, A.; Evtushenko, V.; Filatov, M.;  and Malek, A.\n</span>\n\n  \n\n</span>\n\n  <i>Prostate</i>, 76(1): 68-79.  2016.\n  <span class=\"note\">cited By 62</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&amp;doi=10.1002%2fpros.23101&amp;partnerID=40&amp;md5=10835f69427cb4f1655bac5857581ea3\"\n     onclick=\"log_download('samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&amp;doi=10.1002%2fpros.23101&amp;partnerID=40&amp;md5=10835f69427cb4f1655bac5857581ea3', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/pros.23101\"\n     onclick=\"log_download('samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016', 'http://doi.org/10.1002/pros.23101', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('samsonov-shtam-burdakov-glotov-tsyrlina-berstein-nosov-evtushenko-etal-lectininducedagglutinationmethodofurinaryexosomesisolationfollowedbymirnaanalysisapplicationforprostatecancerdiagnostic-2016')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Samsonov201668,\r\nauthor={Samsonov, R. and Shtam, T. and Burdakov, V. and Glotov, A. and Tsyrlina, E. and Berstein, L. and Nosov, A. and Evtushenko, V. and Filatov, M. and Malek, A.},\r\ntitle={Lectin-induced agglutination method of urinary exosomes isolation followed by mi-RNA analysis: Application for prostate cancer diagnostic},\r\njournal={Prostate},\r\nyear={2016},\r\nvolume={76},\r\nnumber={1},\r\npages={68-79},\r\ndoi={10.1002/pros.23101},\r\nnote={cited By 62},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955181667&amp;doi=10.1002%2fpros.23101&amp;partnerID=40&amp;md5=10835f69427cb4f1655bac5857581ea3},\r\naffiliation={Laboratory of Oncoendocrinology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Genetic Engineering, Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg, Russian Federation; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina, Saint-Petersburg, Russian Federation; Department of Genetics and Biotechnology, Saint Petersburg State University, Saint-Petersburg, Russian Federation; Department of Urology, N.N. Petrov Institute of Oncology, Pesochny, Saint-Petersburg, Russian Federation; Laboratory of Cell Migration and Invasion, Faculty of Medicine in the Galilee, Bar-Ilan University, Safed, Israel},\r\nabstract={BACKGROUND Prostate cancer is the most common cancer in men. Prostate-specific antigen has, however, insufficient diagnostic specificity. Novel complementary diagnostic approaches are greatly needed. MiRNAs are small regulatory RNAs which play an important role in tumorogenesis and are being investigated as a cancer biomarker. In addition to their intracellular regulatory functions, miRNAs are secreted into the extracellular space and can be found in various body fluids, including urine. The stability of extracellular miRNAs is defined by association with proteins, lipoprotein particles, and membrane vesicles. Among the known forms of miRNA packaging, tumour-derived exosome-enclosed miRNAs is thought to reflect the vital activity of cancer cells. The assessment of the exosomal fraction of urinary miRNA may present a new and highly specific method for prostate cancer diagnostics; however, this is challenged by the absence of reliable and inexpensive methods for isolation of exosomes. METHODS Prostate cancer (PC) cell lines and urine samples collected from 35 PC patients and 35 healthy donors were used in the study. Lectins, phytohemagglutinin, and concanavalin A were used to induce agglutination of exosomes. The efficiency of isolation process was evaluated by AFM and DLS assays. The protein content of isolated exosomes was analysed by western blotting. Exosomal RNA was assayed by automated electrophoresis and expression level of selected miRNAs was evaluated by RT-qPCR. The diagnostic potency of the urinary exosomal miRNA assessment was estimated by the ROC method. RESULTS The formation of multi-vesicular agglutinates in urine can be induced by incubation with lectin at a final concentration of 2 mg/ml. These agglutinates contain urinary exosomes and may be pelleted by centrifugation with a relatively low G-force. The analysis of PC-related miRNA in urinary exosomes revealed significant up-regulation of miR-574-3p, miR-141-5p, and miR-21-5p associated with PC. CONCLUSIONS Lectin-induced aggregation is a low-cost and easily performed method for isolation of exosomes from urine. Isolated exosomes can be further analysed in terms of miRNA content. The miRNA profile of urinary exosomes reflects development of prostate cancer and may present a promising diagnostic tool. Prostate 76:68-79, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.},\r\nauthor_keywords={diagnostic;  exosomes;  lectin;  miRNA;  prostate cancer},\r\ncorrespondence_address1={Malek, A.; NN Petrov Institute of Oncology, Leningradskaya 68, Russian Federation; email: anastasia@malek.com},\r\npublisher={John Wiley and Sons Inc.},\r\nissn={02704137},\r\ncoden={PRSTD},\r\npubmed_id={26417675},\r\nlanguage={English},\r\nabbrev_source_title={Prostate},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  BACKGROUND Prostate cancer is the most common cancer in men. Prostate-specific antigen has, however, insufficient diagnostic specificity. Novel complementary diagnostic approaches are greatly needed. MiRNAs are small regulatory RNAs which play an important role in tumorogenesis and are being investigated as a cancer biomarker. In addition to their intracellular regulatory functions, miRNAs are secreted into the extracellular space and can be found in various body fluids, including urine. The stability of extracellular miRNAs is defined by association with proteins, lipoprotein particles, and membrane vesicles. Among the known forms of miRNA packaging, tumour-derived exosome-enclosed miRNAs is thought to reflect the vital activity of cancer cells. The assessment of the exosomal fraction of urinary miRNA may present a new and highly specific method for prostate cancer diagnostics; however, this is challenged by the absence of reliable and inexpensive methods for isolation of exosomes. METHODS Prostate cancer (PC) cell lines and urine samples collected from 35 PC patients and 35 healthy donors were used in the study. Lectins, phytohemagglutinin, and concanavalin A were used to induce agglutination of exosomes. The efficiency of isolation process was evaluated by AFM and DLS assays. The protein content of isolated exosomes was analysed by western blotting. Exosomal RNA was assayed by automated electrophoresis and expression level of selected miRNAs was evaluated by RT-qPCR. The diagnostic potency of the urinary exosomal miRNA assessment was estimated by the ROC method. RESULTS The formation of multi-vesicular agglutinates in urine can be induced by incubation with lectin at a final concentration of 2 mg/ml. These agglutinates contain urinary exosomes and may be pelleted by centrifugation with a relatively low G-force. The analysis of PC-related miRNA in urinary exosomes revealed significant up-regulation of miR-574-3p, miR-141-5p, and miR-21-5p associated with PC. CONCLUSIONS Lectin-induced aggregation is a low-cost and easily performed method for isolation of exosomes from urine. Isolated exosomes can be further analysed in terms of miRNA content. The miRNA profile of urinary exosomes reflects development of prostate cancer and may present a promising diagnostic tool. Prostate 76:68-79, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2015\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2015')\"\n      onkeypress=\"toggleGroup('group_2015')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2015</span>\n      <span class=\"bibbase_group_count\">\n        \n        (5)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&amp;doi=10.1134%2fS1990793115050188&amp;partnerID=40&amp;md5=77037f8ce87c89f036cb23c5eabf2ff7\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&amp;doi=10.1134%2fS1990793115050188&amp;partnerID=40&amp;md5=77037f8ce87c89f036cb23c5eabf2ff7', event);\">\n    Studying navigation signal singularities during auroral disturbances.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Chernous, S.; Shvets, M.; Filatov, M.; Shagimuratov, I.;  and Kalitenkov, N.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Physical Chemistry B</i>, 9(5): 778-784.  2015.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&amp;doi=10.1134%2fS1990793115050188&amp;partnerID=40&amp;md5=77037f8ce87c89f036cb23c5eabf2ff7\"\n     onclick=\"log_download('chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&amp;doi=10.1134%2fS1990793115050188&amp;partnerID=40&amp;md5=77037f8ce87c89f036cb23c5eabf2ff7', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Studying navigation signal singularities during auroral disturbances [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990793115050188\"\n     onclick=\"log_download('chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015', 'http://doi.org/10.1134/S1990793115050188', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('chernous-shvets-filatov-shagimuratov-kalitenkov-studyingnavigationsignalsingularitiesduringauroraldisturbances-2015')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Chernous2015778,\r\nauthor={Chernous, S.A. and Shvets, M.V. and Filatov, M.V. and Shagimuratov, I.I. and Kalitenkov, N.V.},\r\ntitle={Studying navigation signal singularities during auroral disturbances},\r\njournal={Russian Journal of Physical Chemistry B},\r\nyear={2015},\r\nvolume={9},\r\nnumber={5},\r\npages={778-784},\r\ndoi={10.1134/S1990793115050188},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946109488&amp;doi=10.1134%2fS1990793115050188&amp;partnerID=40&amp;md5=77037f8ce87c89f036cb23c5eabf2ff7},\r\naffiliation={Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Apatity, Murmansk oblast, 184209, Russian Federation; Institute of Terrestrial Magnetism, Ionosphere, and Radiowave Propagation, Western Division, Russian Academy of Sciences, pr. Pobedy 41, Kaliningrad, 236017, Russian Federation; Murmansk State Technical University, ul. Sportivnaya 13, Murmansk, 183010, Russian Federation},\r\nabstract={The experimental studies of the GPS and GLONASS satellite signal singularities, depending on the spatial and time distributions of auroras and geomagnetic disturbances that mark the polar ionosphere current state, are presented. The experimental evidences for positioning errors, related to the spatial–time variations in auroral arcs, are demonstrated. These errors of the navigation systems are observed in an increase in the positioning errors and in a violation of the system integrity. It has been indicated that auroral rayed arcs are indicators of disturbances in the navigation system operation. We tried to explain this fact in the scope of the concept of navigation signal phase fluctuations caused by total electron content inhomogeneities along geomagnetic field lines. © 2015, Pleiades Publishing, Ltd.},\r\nauthor_keywords={auroral disturbances;  navigation systems;  positioning error sources},\r\nfunding_details={Russian Foundation for Basic Research14-07-00512},\r\nfunding_details={Russian Foundation for Basic Research4-05-98820r-sever-a},\r\ncorrespondence_address1={Chernous, S.A.; Polar Geophysical Institute, Apatity Division, Kola Scientific Center, Russian Academy of Sciences, ul. Fersmana 14, Russian Federation},\r\npublisher={Maik Nauka Publishing / Springer SBM},\r\nissn={19907931},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Phys. Chem. B},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The experimental studies of the GPS and GLONASS satellite signal singularities, depending on the spatial and time distributions of auroras and geomagnetic disturbances that mark the polar ionosphere current state, are presented. The experimental evidences for positioning errors, related to the spatial–time variations in auroral arcs, are demonstrated. These errors of the navigation systems are observed in an increase in the positioning errors and in a violation of the system integrity. It has been indicated that auroral rayed arcs are indicators of disturbances in the navigation system operation. We tried to explain this fact in the scope of the concept of navigation signal phase fluctuations caused by total electron content inhomogeneities along geomagnetic field lines. © 2015, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&amp;doi=10.1134%2fS1990519X15030086&amp;partnerID=40&amp;md5=0c901448296154db7453d31299ecdd32\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&amp;doi=10.1134%2fS1990519X15030086&amp;partnerID=40&amp;md5=0c901448296154db7453d31299ecdd32', event);\">\n    Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kovalev, R.; Shtam, T.; Karelov, D.; Burdakov, V.; Volnitskiy, A.; Makarov, E.;  and Filatova, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 9(3): 191-197.  2015.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&amp;doi=10.1134%2fS1990519X15030086&amp;partnerID=40&amp;md5=0c901448296154db7453d31299ecdd32\"\n     onclick=\"log_download('kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&amp;doi=10.1134%2fS1990519X15030086&amp;partnerID=40&amp;md5=0c901448296154db7453d31299ecdd32', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X15030086\"\n     onclick=\"log_download('kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015', 'http://doi.org/10.1134/S1990519X15030086', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatova-histonedeacetylaseinhibitorscausetp53dependentinductionofp21waf1intumorcellswithtp53mutations-2015')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kovalev2015191,\r\nauthor={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatova, M.V.},\r\ntitle={Histone deacetylase inhibitors cause TP53-dependent induction of p21/Waf1 in tumor cells with TP53 mutations},\r\njournal={Cell and Tissue Biology},\r\nyear={2015},\r\nvolume={9},\r\nnumber={3},\r\npages={191-197},\r\ndoi={10.1134/S1990519X15030086},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84934918732&amp;doi=10.1134%2fS1990519X15030086&amp;partnerID=40&amp;md5=0c901448296154db7453d31299ecdd32},\r\naffiliation={Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”, Gatchina, Leningrad oblast, 188300, Russian Federation; Department of Biophysics, St. Petersburg State Polytechnical University, St. Petersburg, 194064, Russian Federation; Division of Biosciences, Brunel University, London, United Kingdom},\r\nabstract={The p21/Waf1 protein is one of the main regulators of cell cycle arrest and one of the best-known transcriptional targets of the TP53 protein. Here, we demonstrated that there is activation of expression of the p21/Waf1 gene when the cells were treated with sodium butyrate (NaBu), which is a natural histone deacetylase inhibitor, and investigated whether this phenomenon depends on the presence of a functionally active TP53 protein. For this purpose, we compared the effect of NaBu treatment of human cell lines with different TP53 mutation profiles, including wild-type TP53, single nucleotide substitutions, and the complete absence of the TP53 gene. NaBu activated the TP53 protein via hyperacetylation at the lysine residue K382, without significant changes in the level of protein expression. Western blotting showed that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both TP53 wild-type cells and in cells with single nucleotide substitutions in the central DNA-binding core domain (DBD) of the TP53 protein. At the same time, no p21/Waf1 protein induction was observed in cells with complete deletion of the TP53 gene. However, NaBu was not able to induce p21/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein, but, unexpectedly, it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One possible explanations is that NaBu increases the level of TP53 acetylation and the modified protein is able to establish a new network of protein–protein interactions or trigger conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired. © 2015, Pleiades Publishing, Ltd.},\r\nauthor_keywords={HDAC inhibitors;  p21/Waf1/Cip1;  RNA interference;  sodium butyrate;  TP53;  TP53 mutations},\r\ncorrespondence_address1={Filatova, M.V.; Division of Molecular and Radiation Biophysics, National Research Centre “Kurchatov Institute”Russian Federation},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The p21/Waf1 protein is one of the main regulators of cell cycle arrest and one of the best-known transcriptional targets of the TP53 protein. Here, we demonstrated that there is activation of expression of the p21/Waf1 gene when the cells were treated with sodium butyrate (NaBu), which is a natural histone deacetylase inhibitor, and investigated whether this phenomenon depends on the presence of a functionally active TP53 protein. For this purpose, we compared the effect of NaBu treatment of human cell lines with different TP53 mutation profiles, including wild-type TP53, single nucleotide substitutions, and the complete absence of the TP53 gene. NaBu activated the TP53 protein via hyperacetylation at the lysine residue K382, without significant changes in the level of protein expression. Western blotting showed that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both TP53 wild-type cells and in cells with single nucleotide substitutions in the central DNA-binding core domain (DBD) of the TP53 protein. At the same time, no p21/Waf1 protein induction was observed in cells with complete deletion of the TP53 gene. However, NaBu was not able to induce p21/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein, but, unexpectedly, it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One possible explanations is that NaBu increases the level of TP53 acetylation and the modified protein is able to establish a new network of protein–protein interactions or trigger conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired. © 2015, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&amp;doi=10.1134%2fS1070428015030215&amp;partnerID=40&amp;md5=911c9d5ff7da174e600a9815e94f60e9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&amp;doi=10.1134%2fS1070428015030215&amp;partnerID=40&amp;md5=911c9d5ff7da174e600a9815e94f60e9', event);\">\n    Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Morozkina, S.; Gluzdikov, I.; Drozdov, A.; Selivanov, S.; Kovalev, R.; Filatov, M.;  and Shavva, A.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Organic Chemistry</i>, 51(3): 411-416.  2015.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&amp;doi=10.1134%2fS1070428015030215&amp;partnerID=40&amp;md5=911c9d5ff7da174e600a9815e94f60e9\"\n     onclick=\"log_download('morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&amp;doi=10.1134%2fS1070428015030215&amp;partnerID=40&amp;md5=911c9d5ff7da174e600a9815e94f60e9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1070428015030215\"\n     onclick=\"log_download('morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015', 'http://doi.org/10.1134/S1070428015030215', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('morozkina-gluzdikov-drozdov-selivanov-kovalev-filatov-shavva-synthesisandsomebiologicalpropertiesofsulfamatesderivedfrom8analogsofsteroidalestrogens-2015')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Morozkina2015411,\r\nauthor={Morozkina, S.N. and Gluzdikov, I.A. and Drozdov, A.S. and Selivanov, S.I. and Kovalev, R.A. and Filatov, M.V. and Shavva, A.G.},\r\ntitle={Synthesis and some biological properties of sulfamates derived from 8α-analogs of steroidal estrogens},\r\njournal={Russian Journal of Organic Chemistry},\r\nyear={2015},\r\nvolume={51},\r\nnumber={3},\r\npages={411-416},\r\ndoi={10.1134/S1070428015030215},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928333727&amp;doi=10.1134%2fS1070428015030215&amp;partnerID=40&amp;md5=911c9d5ff7da174e600a9815e94f60e9},\r\naffiliation={St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russian Federation; Petersburg Nuclear Physics Institute, Kurchatov Institute National Research Center, Orlova Roshcha, Gatchina, Leningrad oblast, 188300, Russian Federation; Peter the Great St. Petersburg Polytechnic University, Politekhnicheskaya ul. 29, St. Petersburg, 195251, Russian Federation},\r\nabstract={Sulfamates derived from estrogens of the 8α-series have been synthesized, and their effect on the growth of MCF-7 breast cancer cells has been studied. The best anticancer effect has been found for 7β-methyl-D-homo-6-oxa-8α-estrone sulfamate and 3-methoxy-2,17β-sulfamoyloxy-8α-estra-1,3,5(10)-triene which almost completely inhibit the growth of cancer cells. © 2015 Pleiades Publishing, Ltd.},\r\ncorrespondence_address1={Shavva, A.G.; St. Petersburg State University, Universitetskii pr. 26, Russian Federation},\r\npublisher={Maik Nauka Publishing / Springer SBM},\r\nissn={10704280},\r\ncoden={RJOCE},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Org. Chem.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Sulfamates derived from estrogens of the 8α-series have been synthesized, and their effect on the growth of MCF-7 breast cancer cells has been studied. The best anticancer effect has been found for 7β-methyl-D-homo-6-oxa-8α-estrone sulfamate and 3-methoxy-2,17β-sulfamoyloxy-8α-estra-1,3,5(10)-triene which almost completely inhibit the growth of cancer cells. © 2015 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&amp;partnerID=40&amp;md5=bd66a7bb8e7e0ed78b3edf4820c210f3\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&amp;partnerID=40&amp;md5=bd66a7bb8e7e0ed78b3edf4820c210f3', event);\">\n    Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kovalev, R.; Shtam, T.; Karelov, D.; Burdakov, V.; Volnitskiy, A.; Makarov, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiia</i>, 57(3): 204-211.  2015.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&amp;partnerID=40&amp;md5=bd66a7bb8e7e0ed78b3edf4820c210f3\"\n     onclick=\"log_download('kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&amp;partnerID=40&amp;md5=bd66a7bb8e7e0ed78b3edf4820c210f3', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kovalev-shtam-karelov-burdakov-volnitskiy-makarov-filatov-histonedeacetylaseinhibitorscausethetp53dependentinductionofp21waf1intumorcellscarryingmutationsintp53-2015')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kovalev2015204,\r\nauthor={Kovalev, R.A. and Shtam, T.A. and Karelov, D.V. and Burdakov, V.S. and Volnitskiy, A.V. and Makarov, E.M. and Filatov, M.V.},\r\ntitle={Histone deacetylase inhibitors cause the TP53-dependent induction of p21/Waf1 in tumor cells carrying mutations in TP53},\r\njournal={Tsitologiia},\r\nyear={2015},\r\nvolume={57},\r\nnumber={3},\r\npages={204-211},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930790634&amp;partnerID=40&amp;md5=bd66a7bb8e7e0ed78b3edf4820c210f3},\r\nabstract={p21/Waf1 protein is one of the main cell cycle arrest regulators and one of the most well-known transcriptional targets of TP53 protein. Here, we demonstrated the activation of expression of the p21/Waf1 gene when the cells were treated to sodium butyrate (NaBu)--one of the natural inhibitors of deacetylase, and investigated whether this phenomenon depends on the presence of functionally active TP53 protein. We compared the effect of the NaBu treatment on the human cell line with different TP53 mutation profile, including: wild-type TP53, single nucleotide substitutions, and the complete absence of TP53 gene. NaBu activated the TP53 protein via hyper acetylation at lysine residue K382, without significant changes in the level of protein expression. Western blotting demonstrated that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both the TP53 wild-type cells and in the cells with single nucleotide substitutions in the domain responsible for the binding of TP53 protein to DNA. At the same time, no the p21/Waf1 protein induction was observed in the cells with complete deletion of the TP53 gene. However, NaBu was not able to induce the p2 1/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that the NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein but unexpectedly it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One of the hypothetical explanations is that NaBu increases the level of TP53 acetylation, and the modified protein is able to establish a new network of protein-protein interactions or trigger some conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired.},\r\nissn={00413771},\r\npubmed_id={26021170},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  p21/Waf1 protein is one of the main cell cycle arrest regulators and one of the most well-known transcriptional targets of TP53 protein. Here, we demonstrated the activation of expression of the p21/Waf1 gene when the cells were treated to sodium butyrate (NaBu)–one of the natural inhibitors of deacetylase, and investigated whether this phenomenon depends on the presence of functionally active TP53 protein. We compared the effect of the NaBu treatment on the human cell line with different TP53 mutation profile, including: wild-type TP53, single nucleotide substitutions, and the complete absence of TP53 gene. NaBu activated the TP53 protein via hyper acetylation at lysine residue K382, without significant changes in the level of protein expression. Western blotting demonstrated that the addition of NaBu triggers a significant increase in the p21/Waf1 protein level in both the TP53 wild-type cells and in the cells with single nucleotide substitutions in the domain responsible for the binding of TP53 protein to DNA. At the same time, no the p21/Waf1 protein induction was observed in the cells with complete deletion of the TP53 gene. However, NaBu was not able to induce the p2 1/Waf1 production when the expression of TP53 was transiently knocked down by the p53 siRNA. Overall, our results suggest that the NaBu-dependent induction of p21/Waf1 does require the presence of TP53 protein but unexpectedly it can occur regardless of mutational changes in the domain responsible for the TP53 binding to DNA. One of the hypothetical explanations is that NaBu increases the level of TP53 acetylation, and the modified protein is able to establish a new network of protein-protein interactions or trigger some conformational changes affecting the TP53-dependent transcriptional machinery even when its DNA binding ability is impaired.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&amp;partnerID=40&amp;md5=d9a9fcdb72d174dbbcca3823bb3967f2\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&amp;partnerID=40&amp;md5=d9a9fcdb72d174dbbcca3823bb3967f2', event);\">\n    Exosomal intercellular communications system and its role in the process of metastatic dissemination.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Malek, A.; Berstein, L.; Filatov, M.;  and Belyaev, A.\n</span>\n\n  \n\n</span>\n\n  <i>Voprosy Onkologii</i>, 60(4): 429-436.  2015.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&amp;partnerID=40&amp;md5=d9a9fcdb72d174dbbcca3823bb3967f2\"\n     onclick=\"log_download('malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&amp;partnerID=40&amp;md5=d9a9fcdb72d174dbbcca3823bb3967f2', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Exosomal intercellular communications system and its role in the process of metastatic dissemination [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('malek-berstein-filatov-belyaev-exosomalintercellularcommunicationssystemanditsroleintheprocessofmetastaticdissemination-2015')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Malek2015429,\r\nauthor={Malek, A.V. and Berstein, L.M. and Filatov, M.V. and Belyaev, A.M.},\r\ntitle={Exosomal intercellular communications system and its role in the process of metastatic dissemination},\r\njournal={Voprosy Onkologii},\r\nyear={2015},\r\nvolume={60},\r\nnumber={4},\r\npages={429-436},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923351877&amp;partnerID=40&amp;md5=d9a9fcdb72d174dbbcca3823bb3967f2},\r\naffiliation={Ltd. Oncosystem, Russian Federation; N.N. Petrov Research Institute of Oncology, St. Petersburg, Russian Federation; Kurchatov Institute, Gatchina, Russian Federation},\r\nabstract={Metabolism and information between cells is the basis of the existence of any multicellular organism. Malfunction of the intercellular communication play an important and sometimes decisive role in the pathogenesis of the most diseases, including cancer. According to traditional views, functional integration of individual cells in tissues, organs and organ systems is mediated by the efficient work of regulatory systems: nervous, immune, endocrine. Over the past few years the attention of scientists is attracted the ability of cells to «communicate» with the help of nanoscale vesicular formations, or so-called exosomes. There are accumulated data that the cells of the most tissues secrete exosomes into the intercellular environment, after which, by means of stream ofblood or lymph, exosomes transferred to anatomically distant sites where they are accepted by the other cells. It is showed that the content of exosomes are not random and that vesicular transport may be targeted and to play a significant physiological and even «pathophysiological» role. The aim of this review is the analysis and integration of modern scientific data on the role of exosomes in the process of tumor progression and presentation of possible ways and methods of using these data in the practice of oncology.},\r\nauthor_keywords={Cancer;  Exosomes;  Intercellular contacts;  Metastasis},\r\npublisher={Izdatel&#x27;stvo Meditsina},\r\nissn={05073758},\r\ncoden={VOONA},\r\npubmed_id={25552061},\r\nlanguage={Russian},\r\nabbrev_source_title={Vopr. Onkol.},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Metabolism and information between cells is the basis of the existence of any multicellular organism. Malfunction of the intercellular communication play an important and sometimes decisive role in the pathogenesis of the most diseases, including cancer. According to traditional views, functional integration of individual cells in tissues, organs and organ systems is mediated by the efficient work of regulatory systems: nervous, immune, endocrine. Over the past few years the attention of scientists is attracted the ability of cells to «communicate» with the help of nanoscale vesicular formations, or so-called exosomes. There are accumulated data that the cells of the most tissues secrete exosomes into the intercellular environment, after which, by means of stream ofblood or lymph, exosomes transferred to anatomically distant sites where they are accepted by the other cells. It is showed that the content of exosomes are not random and that vesicular transport may be targeted and to play a significant physiological and even «pathophysiological» role. The aim of this review is the analysis and integration of modern scientific data on the role of exosomes in the process of tumor progression and presentation of possible ways and methods of using these data in the practice of oncology.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2014\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2014')\"\n      onkeypress=\"toggleGroup('group_2014')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2014</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&amp;doi=10.1134%2fS1990519X14050101&amp;partnerID=40&amp;md5=761056e34f65f3402cadffe380c42b4e\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&amp;doi=10.1134%2fS1990519X14050101&amp;partnerID=40&amp;md5=761056e34f65f3402cadffe380c42b4e', event);\">\n    Aberrant expression of the sox2 gene in malignant gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Volnitskiy, A.; Semenova, E.; Shtam, T.; Kovalev, R.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 8(5): 368-373.  2014.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&amp;doi=10.1134%2fS1990519X14050101&amp;partnerID=40&amp;md5=761056e34f65f3402cadffe380c42b4e\"\n     onclick=\"log_download('volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&amp;doi=10.1134%2fS1990519X14050101&amp;partnerID=40&amp;md5=761056e34f65f3402cadffe380c42b4e', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Aberrant expression of the sox2 gene in malignant gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X14050101\"\n     onclick=\"log_download('volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014', 'http://doi.org/10.1134/S1990519X14050101', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('volnitskiy-semenova-shtam-kovalev-filatov-aberrantexpressionofthesox2geneinmalignantgliomas-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Volnitskiy2014368,\r\nauthor={Volnitskiy, A.V. and Semenova, E.V. and Shtam, T.A. and Kovalev, R.A. and Filatov, M.V.},\r\ntitle={Aberrant expression of the sox2 gene in malignant gliomas},\r\njournal={Cell and Tissue Biology},\r\nyear={2014},\r\nvolume={8},\r\nnumber={5},\r\npages={368-373},\r\ndoi={10.1134/S1990519X14050101},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918775524&amp;doi=10.1134%2fS1990519X14050101&amp;partnerID=40&amp;md5=761056e34f65f3402cadffe380c42b4e},\r\naffiliation={Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\r\nabstract={Both genetic and epigenetic changes underlie the mechanisms of tumor initiation and progression. In this study, we analyzed sox2 gene expression and its epigenetic changes in primary cultures of malignant gliomas. The sox2 expression was detected in most (74%) gliomas, but not in morphologically normal brain tissue. These facts point to relationships between the sox2 transcription activity and the process of glioma malignant transformation. It was demonstrated that association of different areas of the sox2 gene with important epigenetic markers—posttranslational modifications of H3 histone H3K4ac and H3K9met3—did not correlate with sox2 expression. However, it suggests stochastic regulation of sox2 gene expression in malignant gliomas. © 2014, Pleiades Publishing, Ltd.},\r\nauthor_keywords={aberrant gene expression;  gliomas;  posttranslational H3 histone modifications;  Sox2},\r\ncorrespondence_address1={Filatov, M.V.; Konstantinov St. Petersburg Nuclear Physics InstituteRussian Federation},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Both genetic and epigenetic changes underlie the mechanisms of tumor initiation and progression. In this study, we analyzed sox2 gene expression and its epigenetic changes in primary cultures of malignant gliomas. The sox2 expression was detected in most (74%) gliomas, but not in morphologically normal brain tissue. These facts point to relationships between the sox2 transcription activity and the process of glioma malignant transformation. It was demonstrated that association of different areas of the sox2 gene with important epigenetic markers—posttranslational modifications of H3 histone H3K4ac and H3K9met3—did not correlate with sox2 expression. However, it suggests stochastic regulation of sox2 gene expression in malignant gliomas. © 2014, Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&amp;doi=10.1134%2fS1990750814030111&amp;partnerID=40&amp;md5=b61f4ee9207a407172433dd9dd39a941\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&amp;doi=10.1134%2fS1990750814030111&amp;partnerID=40&amp;md5=b61f4ee9207a407172433dd9dd39a941', event);\">\n    Development of barcode and proteome profiling of glioblastoma.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry</i>, 8(3): 243-251.  2014.\n  <span class=\"note\">cited By 7</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&amp;doi=10.1134%2fS1990750814030111&amp;partnerID=40&amp;md5=b61f4ee9207a407172433dd9dd39a941\"\n     onclick=\"log_download('anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906841739&amp;doi=10.1134%2fS1990750814030111&amp;partnerID=40&amp;md5=b61f4ee9207a407172433dd9dd39a941', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Development of barcode and proteome profiling of glioblastoma [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990750814030111\"\n     onclick=\"log_download('anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014', 'http://doi.org/10.1134/S1990750814030111', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-developmentofbarcodeandproteomeprofilingofglioblastoma-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it the fourth biggest cause of cancer death. In our experiments, we used several glioblastoma cell lines to obtain proteomics information specific for this disease. 2DE separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us to detecting more than 600 protein spots and identifying more than 130 of them. Proteome profiles in normal and glioblastoma cell lines are very similar but levels of several proteins have prominent differences between norm and cancer. Among these proteins are alpha-enolase (ENOA-HUMAN), pyruvate kinase M1/M2 (KPYM-HUMAN), cofilin 1 (COF1-HUMAN), translationally-controlled tumor protein TCTP-HUMAN, annexin 1 (ANXA1-HUMAN), PCNA (PCNA-HUMAN), p53 (TP53-HUMAN) and others. Most interesting results were obtained about protein p53. Its level was dramatically up-regulated and enriched by multiple additional isoforms in all glioblastoma cell lines. An immunological analysis (Western blot) of three hub-proteins (p53, 14-3-3, PCNA) allowed us to creating the minimal barcode of glioblastoma cell lines. These preliminary data point to this barcode as a promising diagnostic tool for testing of the biological fluids from patients. © 2014 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&amp;doi=10.1016%2fj.devcel.2014.08.006&amp;partnerID=40&amp;md5=a22252e06e433a5220b557e968f8395f\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&amp;doi=10.1016%2fj.devcel.2014.08.006&amp;partnerID=40&amp;md5=a22252e06e433a5220b557e968f8395f', event);\">\n    The CDC42-interacting protein 4 controls epithelial cell cohesion and tumor dissemination.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Developmental Cell</i>, 30(5): 553-568.  2014.\n  <span class=\"note\">cited By 29</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&amp;doi=10.1016%2fj.devcel.2014.08.006&amp;partnerID=40&amp;md5=a22252e06e433a5220b557e968f8395f\"\n     onclick=\"log_download('anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908615203&amp;doi=10.1016%2fj.devcel.2014.08.006&amp;partnerID=40&amp;md5=a22252e06e433a5220b557e968f8395f', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The CDC42-interacting protein 4 controls epithelial cell cohesion and tumor dissemination [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.devcel.2014.08.006\"\n     onclick=\"log_download('anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014', 'http://doi.org/10.1016/j.devcel.2014.08.006', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-thecdc42interactingprotein4controlsepithelialcellcohesionandtumordissemination-2014')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The role of endocytic proteins and the molecular mechanisms underlying epithelial cell cohesion and tumor dissemination are not well understood. Here, we report that the endocytic F-BAR-containing CDC42-interacting protein 4 (CIP4) is required for ERBB2- and TGF-β1-induced cell scattering, breast cancer (BC) cell motility and invasion into 3D matrices, and conversion from ductal breast carcinoma insitu to invasive carcinoma in mouse xenograft models. CIP4 promotes the formation of an E-cadherin-CIP4-SRC complex that controls SRC activation, E-cadherin endocytosis, and localized phosphorylation of the myosin light chain kinase, thereby impinging on the actomyosin contractility required to generate tangential forces to break cell-cell junctions. CIP4 is upregulated in ERBB2-positive human BC, correlates with increased distant metastasis, and is an independent predictor of poor disease outcome in subsets of BC patients. Thus, it critically controls cell-cell cohesion and is required for the acquisition of an invasive phenotype in breast tumors. © 2014 Elsevier Inc.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2013\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2013')\"\n      onkeypress=\"toggleGroup('group_2013')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2013</span>\n      <span class=\"bibbase_group_count\">\n        \n        (6)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&amp;doi=10.1186%2f1478-811X-11-88&amp;partnerID=40&amp;md5=da53d624b8be3bfd1494f309cb115de5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&amp;doi=10.1186%2f1478-811X-11-88&amp;partnerID=40&amp;md5=da53d624b8be3bfd1494f309cb115de5', event);\">\n    Exosomes are natural carriers of exogenous siRNA to human cells in vitro.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Kovalev, R.; Varfolomeeva, E.; Makarov, E.; Kil, Y.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell Communication and Signaling</i>, 11(1).  2013.\n  <span class=\"note\">cited By 160</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&amp;doi=10.1186%2f1478-811X-11-88&amp;partnerID=40&amp;md5=da53d624b8be3bfd1494f309cb115de5\"\n     onclick=\"log_download('shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&amp;doi=10.1186%2f1478-811X-11-88&amp;partnerID=40&amp;md5=da53d624b8be3bfd1494f309cb115de5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Exosomes are natural carriers of exogenous siRNA to human cells in vitro [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1186/1478-811X-11-88\"\n     onclick=\"log_download('shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013', 'http://doi.org/10.1186/1478-811X-11-88', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-kovalev-varfolomeeva-makarov-kil-filatov-exosomesarenaturalcarriersofexogenoussirnatohumancellsinvitro-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2013,\r\nauthor={Shtam, T.A. and Kovalev, R.A. and Varfolomeeva, E.Y. and Makarov, E.M. and Kil, Y.V. and Filatov, M.V.},\r\ntitle={Exosomes are natural carriers of exogenous siRNA to human cells in vitro},\r\njournal={Cell Communication and Signaling},\r\nyear={2013},\r\nvolume={11},\r\nnumber={1},\r\ndoi={10.1186/1478-811X-11-88},\r\nart_number={88},\r\nnote={cited By 160},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887703287&amp;doi=10.1186%2f1478-811X-11-88&amp;partnerID=40&amp;md5=da53d624b8be3bfd1494f309cb115de5},\r\naffiliation={Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; School of Health Sciences and Social Care, Brunel University, Uxbridge UB8 3PH, United Kingdom; Department of Biophysics, St. Petersburg State Polytechnical University, St.-Petersburg 195251, Russian Federation},\r\nabstract={Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.},\r\nauthor_keywords={Cancer therapy;  Drug delivery system;  Exosomes;  RAD51;  RNA interference (RNAi)},\r\ncorrespondence_address1={Filatov, M.V.; Division of Molecular and Radiation Biophysics, SFBI Petersburg Nuclear Physics Institute, Gatchina 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\r\nissn={1478811X},\r\npubmed_id={24245560},\r\nlanguage={English},\r\nabbrev_source_title={Cell Commun. Signal.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful in gene cancer therapy to deliver therapeutic short interfering RNA (siRNA) to the target cells. Despite the promise of RNA interference (RNAi) for use in therapy, several technical obstacles must be overcome. Exogenous siRNA is prone to degradation, has a limited ability to cross cell membranes and may induce an immune response. Naturally occurring RNA carriers, such as exosomes, might provide an untapped source of effective delivery strategies. Results: This study demonstrates that exosomes can deliver siRNA to recipient cells in vitro. The different strategies were used to introduce siRNAs into human exosomes of various origins. The delivery of fluorescently labeled siRNA via exosomes to cells was confirmed using confocal microscopy and flow cytometry. Two different siRNAs against RAD51 and RAD52 were used to transfect into the exosomes for therapeutic delivery into target cells. The exosome-delivered siRNAs were effective at causing post-transcriptional gene silencing in recipient cells. Moreover, the exosome-delivered siRNA against RAD51 was functional and caused the massive reproductive cell death of recipient cancer cells. Conclusions: The results strongly suggest that exosomes effectively delivered the siRNA into the target cells. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated in vitro by the strong knockdown of RAD51, a prospective therapeutic target for cancer cells. The results give an additional evidence of the ability to use human exosomes as vectors in cancer therapy, including RNAi-based gene therapy. © 2013 Shtam et al.; licensee BioMed Central Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&amp;doi=10.1134%2fS1027451013060414&amp;partnerID=40&amp;md5=90679420c913620df35e1385632cd253\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&amp;doi=10.1134%2fS1027451013060414&amp;partnerID=40&amp;md5=90679420c913620df35e1385632cd253', event);\">\n    Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Yung, I.; Pantina, R.; Lebedev, D.; Filatov, M.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Journal of Surface Investigation</i>, 7(6): 1137-1142.  2013.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&amp;doi=10.1134%2fS1027451013060414&amp;partnerID=40&amp;md5=90679420c913620df35e1385632cd253\"\n     onclick=\"log_download('yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&amp;doi=10.1134%2fS1027451013060414&amp;partnerID=40&amp;md5=90679420c913620df35e1385632cd253', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1027451013060414\"\n     onclick=\"log_download('yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013', 'http://doi.org/10.1134/S1027451013060414', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('yung-pantina-lebedev-filatov-isaevivanov-distancedistributionfunctionsofreplicationoriginsinhelaandgliomahumancellsaccordingtothedataofconfocalmicroscopy-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Yung20131137,\r\nauthor={Yung, I.A. and Pantina, R.A. and Lebedev, D.V. and Filatov, M.V. and Isaev-Ivanov, V.V.},\r\ntitle={Distance distribution functions of replication origins in HeLa and glioma human cells according to the data of confocal microscopy},\r\njournal={Journal of Surface Investigation},\r\nyear={2013},\r\nvolume={7},\r\nnumber={6},\r\npages={1137-1142},\r\ndoi={10.1134/S1027451013060414},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894613123&amp;doi=10.1134%2fS1027451013060414&amp;partnerID=40&amp;md5=90679420c913620df35e1385632cd253},\r\naffiliation={National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation},\r\nabstract={The distribution of replication origins in the nuclei of different cells is studied by confocal microscopy. Based on the obtained images, three-dimensional maps of the positions of the origin centers is constructed and the distribution functions of the pair distances between them are calculated. It is established that the distance distribution function for HeLa and glioma human cells is linear at sizes up to 2 μm, which indicates that the size of the origin system is close to 2. The amplitude of the distance distribution function at small sizes has a power dependence on the nucleus size and is inversely proportional to the nucleus volume to the power of 0.9. Thus, the replication-origin distribution in a nucleus cannot be described by a model with a single Hausdorff dimension in the whole range of sizes. © 2013 Pleiades Publishing, Ltd.},\r\ncorrespondence_address1={Yung, I. A.; National Research Center Kurchatov Institute, Petersburg Nuclear Physics Institute, Gatchina, Leningrad oblast, 188300, Russian Federation; email: Igor.Yung@rambler.ru},\r\nissn={10274510},\r\nlanguage={English},\r\nabbrev_source_title={J. Surf. Invest.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The distribution of replication origins in the nuclei of different cells is studied by confocal microscopy. Based on the obtained images, three-dimensional maps of the positions of the origin centers is constructed and the distribution functions of the pair distances between them are calculated. It is established that the distance distribution function for HeLa and glioma human cells is linear at sizes up to 2 μm, which indicates that the size of the origin system is close to 2. The amplitude of the distance distribution function at small sizes has a power dependence on the nucleus size and is inversely proportional to the nucleus volume to the power of 0.9. Thus, the replication-origin distribution in a nucleus cannot be described by a model with a single Hausdorff dimension in the whole range of sizes. © 2013 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&amp;doi=10.1128%2fJVI.00462-13&amp;partnerID=40&amp;md5=d7ebaa5d3acd911b11d1eafd42e0592e\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&amp;doi=10.1128%2fJVI.00462-13&amp;partnerID=40&amp;md5=d7ebaa5d3acd911b11d1eafd42e0592e', event);\">\n    Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Bruder, J.; Chen, P.; Semenova, E.; Thomas, C.; Konovalova, S.; Ekberg, G.; Ettyreddy, D.; McVey, D.; Gall, J.; King, C.;  and Brough, D.\n</span>\n\n  \n\n</span>\n\n  <i>Journal of Virology</i>, 87(17): 9661-9671.  2013.\n  <span class=\"note\">cited By 8</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&amp;doi=10.1128%2fJVI.00462-13&amp;partnerID=40&amp;md5=d7ebaa5d3acd911b11d1eafd42e0592e\"\n     onclick=\"log_download('bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&amp;doi=10.1128%2fJVI.00462-13&amp;partnerID=40&amp;md5=d7ebaa5d3acd911b11d1eafd42e0592e', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1128/JVI.00462-13\"\n     onclick=\"log_download('bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013', 'http://doi.org/10.1128/JVI.00462-13', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('bruder-chen-semenova-thomas-konovalova-ekberg-ettyreddy-mcvey-etal-identificationofasuppressormutationthatimprovestheyieldsofhexonmodifiedadenovirusvectors-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Bruder20139661,\r\nauthor={Bruder, J.T. and Chen, P. and Semenova, E. and Thomas, C.A. and Konovalova, S. and Ekberg, G. and Ettyreddy, D. and McVey, D. and Gall, J.G. and King, C.R. and Brough, D.E.},\r\ntitle={Identification of a suppressor mutation that improves the yields of hexon-modified adenovirus vectors},\r\njournal={Journal of Virology},\r\nyear={2013},\r\nvolume={87},\r\nnumber={17},\r\npages={9661-9671},\r\ndoi={10.1128/JVI.00462-13},\r\nnote={cited By 8},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883284588&amp;doi=10.1128%2fJVI.00462-13&amp;partnerID=40&amp;md5=d7ebaa5d3acd911b11d1eafd42e0592e},\r\naffiliation={GenVec, Inc., Gaithersburg, MD, United States; International AIDS Vaccine Initiative, AIDS Vaccine Design and Development Laboratory, Brooklyn Army Terminal, Brooklyn, NY, United States},\r\nabstract={We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior exposure to Ad5. However, hexon-modified Ad5 vectors displayed an approximate 10-fold growth defect in complementing cells, making potential vaccine costs unacceptably high. Replacing hypervariable regions (HVRs) 1, 2, 4, and 5 with the equivalent HVRs from Ad43 was sufficient to avoid Ad5 preexisting immunity and retain full vaccine potential. However, the resulting vector displayed the same growth defect as the hexon-modified vector carrying all 9 HVRs from Ad43. The growth defect is likely due to a defect in capsid assembly, since DNA replication and late protein accumulation were normal in these vectors. We determined that the hexon-modified vectors have a 32°C cold-sensitive phenotype and selected revertants that restored vector productivity. Genome sequencing identified a single base change resulting in a threonine-to-methionine amino acid substitution at the position equivalent to residue 342 of the wild-type protein. This mutation has a suppressor phenotype (SP), since cloning it into our Ad5 vector containing all nine hypervariable regions from Ad43, Ad5.H(43m-43), increased yields over the version without the SP mutation. This growth improvement was also shown for an Ad5-based hexon-modified vector that carried the hexon hypervariable regions of Ad48, indicating that the SP mutation may have broad applicability for improving the productivity of different hexon-modified vectors. © 2013, American Society for Microbiology.},\r\nfunding_details={National Institutes of Health1R43 AI077309-01},\r\ncorrespondence_address1={Bruder, J.T.; GenVec, Inc., Gaithersburg, MD, United States; email: joetbruder@gmail.com},\r\nissn={0022538X},\r\ncoden={JOVIA},\r\npubmed_id={23824800},\r\nlanguage={English},\r\nabbrev_source_title={J. Virol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  We have generated hexon-modified adenovirus serotype 5 (Ad5) vectors that are not neutralized by Ad5-specific neutralizing antibodies in mice. These vectors are attractive for the advancement of vaccine products because of their potential for inducing robust antigen-specific immune responses in people with prior exposure to Ad5. However, hexon-modified Ad5 vectors displayed an approximate 10-fold growth defect in complementing cells, making potential vaccine costs unacceptably high. Replacing hypervariable regions (HVRs) 1, 2, 4, and 5 with the equivalent HVRs from Ad43 was sufficient to avoid Ad5 preexisting immunity and retain full vaccine potential. However, the resulting vector displayed the same growth defect as the hexon-modified vector carrying all 9 HVRs from Ad43. The growth defect is likely due to a defect in capsid assembly, since DNA replication and late protein accumulation were normal in these vectors. We determined that the hexon-modified vectors have a 32°C cold-sensitive phenotype and selected revertants that restored vector productivity. Genome sequencing identified a single base change resulting in a threonine-to-methionine amino acid substitution at the position equivalent to residue 342 of the wild-type protein. This mutation has a suppressor phenotype (SP), since cloning it into our Ad5 vector containing all nine hypervariable regions from Ad43, Ad5.H(43m-43), increased yields over the version without the SP mutation. This growth improvement was also shown for an Ad5-based hexon-modified vector that carried the hexon hypervariable regions of Ad48, indicating that the SP mutation may have broad applicability for improving the productivity of different hexon-modified vectors. © 2013, American Society for Microbiology.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&amp;doi=10.1134%2fS1990519X13040123&amp;partnerID=40&amp;md5=d829132a07f3116bdb8a07052fe396d0\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-filatov-geneticandepigeneticmarkersofgliomas-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&amp;doi=10.1134%2fS1990519X13040123&amp;partnerID=40&amp;md5=d829132a07f3116bdb8a07052fe396d0', event);\">\n    Genetic and epigenetic markers of gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 7(4): 303-313.  2013.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&amp;doi=10.1134%2fS1990519X13040123&amp;partnerID=40&amp;md5=d829132a07f3116bdb8a07052fe396d0\"\n     onclick=\"log_download('semenova-filatov-geneticandepigeneticmarkersofgliomas-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&amp;doi=10.1134%2fS1990519X13040123&amp;partnerID=40&amp;md5=d829132a07f3116bdb8a07052fe396d0', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Genetic and epigenetic markers of gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X13040123\"\n     onclick=\"log_download('semenova-filatov-geneticandepigeneticmarkersofgliomas-2013', 'http://doi.org/10.1134/S1990519X13040123', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-filatov-geneticandepigeneticmarkersofgliomas-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2013303,\r\nauthor={Semenova, E.V. and Filatov, M.V.},\r\ntitle={Genetic and epigenetic markers of gliomas},\r\njournal={Cell and Tissue Biology},\r\nyear={2013},\r\nvolume={7},\r\nnumber={4},\r\npages={303-313},\r\ndoi={10.1134/S1990519X13040123},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881061744&amp;doi=10.1134%2fS1990519X13040123&amp;partnerID=40&amp;md5=d829132a07f3116bdb8a07052fe396d0},\r\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},\r\nabstract={Malignant gliomas are aggressive and highly invasive tumors. Various genetic and epigenetic changes are common for these tumors. Mostly they concern the genes involved in cell-cycle regulation, apoptotic pathways, cell invasion, angiogenesis, and cell metabolism. The role of epigenetic mechanisms in glioma malignant transformation, despite recent progress, is uncertain and remains under intense study. This review describes the mechanisms of epigenetic regulation of gene expression, including posttranslational modifications of histones, DNA methylation in promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used for diagnostics and new therapeutic approaches are also discussed. © 2013 Pleiades Publishing, Ltd.},\r\nauthor_keywords={epigenetic alterations;  genetic particularities;  gliomas},\r\ncorrespondence_address1={Semenova, E. V.; Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: semenova_el.spb@mail.ru},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Malignant gliomas are aggressive and highly invasive tumors. Various genetic and epigenetic changes are common for these tumors. Mostly they concern the genes involved in cell-cycle regulation, apoptotic pathways, cell invasion, angiogenesis, and cell metabolism. The role of epigenetic mechanisms in glioma malignant transformation, despite recent progress, is uncertain and remains under intense study. This review describes the mechanisms of epigenetic regulation of gene expression, including posttranslational modifications of histones, DNA methylation in promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used for diagnostics and new therapeutic approaches are also discussed. © 2013 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&amp;doi=10.1134%2fS1995423913020055&amp;partnerID=40&amp;md5=ae022d86a3cfca1a839a87e852e65b87\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&amp;doi=10.1134%2fS1995423913020055&amp;partnerID=40&amp;md5=ae022d86a3cfca1a839a87e852e65b87', event);\">\n    Using Zernike moments for analysis of images.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Babkina, L.; Garmai, Y.; Lebedev, D.; Pantina, R.; Filatov, M.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Numerical Analysis and Applications</i>, 6(2): 131-144.  2013.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&amp;doi=10.1134%2fS1995423913020055&amp;partnerID=40&amp;md5=ae022d86a3cfca1a839a87e852e65b87\"\n     onclick=\"log_download('babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&amp;doi=10.1134%2fS1995423913020055&amp;partnerID=40&amp;md5=ae022d86a3cfca1a839a87e852e65b87', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Using Zernike moments for analysis of images [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1995423913020055\"\n     onclick=\"log_download('babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013', 'http://doi.org/10.1134/S1995423913020055', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('babkina-garmai-lebedev-pantina-filatov-isaevivanov-usingzernikemomentsforanalysisofimages-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Babkina2013131,\r\nauthor={Babkina, L.A. and Garmai, Y.P. and Lebedev, D.V. and Pantina, R.A. and Filatov, M.V. and Isaev-Ivanov, V.V.},\r\ntitle={Using Zernike moments for analysis of images},\r\njournal={Numerical Analysis and Applications},\r\nyear={2013},\r\nvolume={6},\r\nnumber={2},\r\npages={131-144},\r\ndoi={10.1134/S1995423913020055},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878847142&amp;doi=10.1134%2fS1995423913020055&amp;partnerID=40&amp;md5=ae022d86a3cfca1a839a87e852e65b87},\r\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Biophysics Scientific-Educational Structure at the St. Petersburg State Polytechnic University, Konstantinov Petersburg Nuclear Physics Institute, St. Petersburg, Russian Federation},\r\nabstract={A method for analyzing AFM images of the cell nuclei of higher organisms by expanding these images by Zernike moments is proposed. This method allows for expanding the pilot image by Zernike moments whose spatial harmonics are Zernike polynomials. It is shown that the reverse procedure of image reconstruction using Zernike polynomials converges to the experimental image and the expansion amplitude is a quantitative spectral characteristic in comparing the morphological features of different images. It is shown that expansion amplitudes can be used as input vectors for cluster analysis of images by PCA. © 2013 Pleiades Publishing, Ltd.},\r\nauthor_keywords={atomic force microscopy;  cell nuclei of higher organisms;  image analysis;  PCA;  Zernike moments},\r\nfunding_details={Ministry of Education and Science of the Russian Federation2.2.1.1/1166},\r\n}</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A method for analyzing AFM images of the cell nuclei of higher organisms by expanding these images by Zernike moments is proposed. This method allows for expanding the pilot image by Zernike moments whose spatial harmonics are Zernike polynomials. It is shown that the reverse procedure of image reconstruction using Zernike polynomials converges to the experimental image and the expansion amplitude is a quantitative spectral characteristic in comparing the morphological features of different images. It is shown that expansion amplitudes can be used as input vectors for cluster analysis of images by PCA. © 2013 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&amp;partnerID=40&amp;md5=9827c4d438c05b1e4f0ec867aae49af4\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-filatov-geneticandepigeneticmarkersofgliomas-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&amp;partnerID=40&amp;md5=9827c4d438c05b1e4f0ec867aae49af4', event);\">\n    Genetic and epigenetic markers of gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 55(5): 290-299.  2013.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&amp;partnerID=40&amp;md5=9827c4d438c05b1e4f0ec867aae49af4\"\n     onclick=\"log_download('semenova-filatov-geneticandepigeneticmarkersofgliomas-2013', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&amp;partnerID=40&amp;md5=9827c4d438c05b1e4f0ec867aae49af4', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Genetic and epigenetic markers of gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-filatov-geneticandepigeneticmarkersofgliomas-2013\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-filatov-geneticandepigeneticmarkersofgliomas-2013')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2013290,\r\nauthor={Semenova, E.V. and Filatov, M.V.},\r\ntitle={Genetic and epigenetic markers of gliomas},\r\njournal={Tsitologiya},\r\nyear={2013},\r\nvolume={55},\r\nnumber={5},\r\npages={290-299},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897023483&amp;partnerID=40&amp;md5=9827c4d438c05b1e4f0ec867aae49af4},\r\naffiliation={B. P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russian Federation},\r\nabstract={Malignant gliomas, aggressive and highly invasive tumors with a great number of genetic and epigenetic alterations in genes involved in the cell cycle regulation, the apoptotic pathways, cell invasion ability, and angiogenesis, are considered to be among the deadliest of human cancers. The role of epigenetic mechanisms in the pathogenesis of malignant transformation despite recent progress is not yet clear elucidated and remains under intensive study. This review describes the mechanisms of epigenetic regulation of gene expression, including post-translational modification of histones, DNA methylation in the promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used in the development of diagnostics and new therapeutic approaches are also discussed.},\r\nauthor_keywords={Epigenetic alterations;  Genetic particularity;  Gliomas},\r\npublisher={Maik Nauka Publishing / Springer SBM},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={24592735},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Malignant gliomas, aggressive and highly invasive tumors with a great number of genetic and epigenetic alterations in genes involved in the cell cycle regulation, the apoptotic pathways, cell invasion ability, and angiogenesis, are considered to be among the deadliest of human cancers. The role of epigenetic mechanisms in the pathogenesis of malignant transformation despite recent progress is not yet clear elucidated and remains under intensive study. This review describes the mechanisms of epigenetic regulation of gene expression, including post-translational modification of histones, DNA methylation in the promoter regions, and microRNA regulation. The genetic and epigenetic factors driving the pathogenesis of gliomas in their possible mutual influence and the potential epigenetic targets that can be used in the development of diagnostics and new therapeutic approaches are also discussed.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2012\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2012')\"\n      onkeypress=\"toggleGroup('group_2012')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2012</span>\n      <span class=\"bibbase_group_count\">\n        \n        (14)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&amp;partnerID=40&amp;md5=330a4b66718da144b754fc8a404b3fd9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&amp;partnerID=40&amp;md5=330a4b66718da144b754fc8a404b3fd9', event);\">\n    In vitro potential of histone deacetylase inhibitors for anticancer therapy.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kovalev, R.; Shtam, T.; Ibatullin, F.; Bondarev, G.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Voprosy Onkologii</i>, 58(6): 800-807.  2012.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&amp;partnerID=40&amp;md5=330a4b66718da144b754fc8a404b3fd9\"\n     onclick=\"log_download('kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&amp;partnerID=40&amp;md5=330a4b66718da144b754fc8a404b3fd9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"In vitro potential of histone deacetylase inhibitors for anticancer therapy [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kovalev-shtam-ibatullin-bondarev-filatov-invitropotentialofhistonedeacetylaseinhibitorsforanticancertherapy-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kovalev2012800,\r\nauthor={Kovalev, R.A. and Shtam, T.A. and Ibatullin, F.M. and Bondarev, G.N. and Filatov, M.V.},\r\ntitle={In vitro potential of histone deacetylase inhibitors for anticancer therapy},\r\njournal={Voprosy Onkologii},\r\nyear={2012},\r\nvolume={58},\r\nnumber={6},\r\npages={800-807},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875510733&amp;partnerID=40&amp;md5=330a4b66718da144b754fc8a404b3fd9},\r\naffiliation={Nuclear Physics Institute, Saint-Petersburg, Russian Federation},\r\nabstract={Research during the past decade has shown that epigenetic events have a key role in carcinogenesis and tumour progression. Histone deacetylase inhibitors (HDACi) comprise structurally diverse compounds that are a group of targeted epigenetic anticancer agents. Here we explored the in vitro efficacy of HDACi such as sodium butyrate (BuNa), valproic acid (VaNa) and several novel HDAC inhibitors for the treatment of cancer. Both BuNa and VaNa inhibited cancer cell proliferation in a time - and dose-dependent fashion. In the present study we demonstrated the significant effect of two novel HDACi, Adipo or BuNHOH, able to induce apoptosis of cancer cells, but not of normal line. Since HDAC inhibitors have been proposed as radio - or chemosensitizers in cancer therapy, we have studied the radiosensitizing effect of sodium butyrate on cancer cells. The combination of BuNa and radiation significantly inhibited tumor cell growth. Besides, combining Cisplatin or Gemzar with HDAC inhibitors results in synergistic antiproliferative activity that could be therapeutically exploited. These results suggest that HDACi acts as an antitumor agent and that combining HDAC inhibitors with radio or - chemotherapeutic strategy may provide a novel chemotherapeutic treatment of cancers insensitive to traditional antitumor agents.},\r\ncorrespondence_address1={Kovalev, R.A.; Nuclear Physics Institute, Saint-Petersburg, Russian Federation},\r\nissn={05073758},\r\ncoden={VOONA},\r\npubmed_id={23600307},\r\nlanguage={Russian},\r\nabbrev_source_title={Vopr. Onkol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Research during the past decade has shown that epigenetic events have a key role in carcinogenesis and tumour progression. Histone deacetylase inhibitors (HDACi) comprise structurally diverse compounds that are a group of targeted epigenetic anticancer agents. Here we explored the in vitro efficacy of HDACi such as sodium butyrate (BuNa), valproic acid (VaNa) and several novel HDAC inhibitors for the treatment of cancer. Both BuNa and VaNa inhibited cancer cell proliferation in a time - and dose-dependent fashion. In the present study we demonstrated the significant effect of two novel HDACi, Adipo or BuNHOH, able to induce apoptosis of cancer cells, but not of normal line. Since HDAC inhibitors have been proposed as radio - or chemosensitizers in cancer therapy, we have studied the radiosensitizing effect of sodium butyrate on cancer cells. The combination of BuNa and radiation significantly inhibited tumor cell growth. Besides, combining Cisplatin or Gemzar with HDAC inhibitors results in synergistic antiproliferative activity that could be therapeutically exploited. These results suggest that HDACi acts as an antitumor agent and that combining HDAC inhibitors with radio or - chemotherapeutic strategy may provide a novel chemotherapeutic treatment of cancers insensitive to traditional antitumor agents.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&amp;partnerID=40&amp;md5=81ac9932d0f3ad831e25dbfd7c3c5d62\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&amp;partnerID=40&amp;md5=81ac9932d0f3ad831e25dbfd7c3c5d62', event);\">\n    P73 gene expression in gliomas.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Volnyzky, A.; Vinogradskaya, G.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Voprosy Onkologii</i>, 58(4): 545-548.  2012.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&amp;partnerID=40&amp;md5=81ac9932d0f3ad831e25dbfd7c3c5d62\"\n     onclick=\"log_download('volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&amp;partnerID=40&amp;md5=81ac9932d0f3ad831e25dbfd7c3c5d62', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"P73 gene expression in gliomas [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('volnyzky-vinogradskaya-filatov-p73geneexpressioningliomas-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Volnyzky2012545,\r\nauthor={Volnyzky, A.V. and Vinogradskaya, G.R. and Filatov, M.V.},\r\ntitle={P73 gene expression in gliomas},\r\njournal={Voprosy Onkologii},\r\nyear={2012},\r\nvolume={58},\r\nnumber={4},\r\npages={545-548},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875825783&amp;partnerID=40&amp;md5=81ac9932d0f3ad831e25dbfd7c3c5d62},\r\naffiliation={B.P.Konstantinov Saint-Petersburg, Institute for Nuclear Physics, Russian Federation},\r\nabstract={We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.},\r\ncorrespondence_address1={Volnyzky, A.V.; B.P.Konstantinov Saint-Petersburg, Institute for Nuclear PhysicsRussian Federation},\r\nissn={05073758},\r\ncoden={VOONA},\r\npubmed_id={23607213},\r\nlanguage={Russian},\r\nabbrev_source_title={Vopr. Onkol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&amp;partnerID=40&amp;md5=fe62933099f2553f60478e65194b7c41\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&amp;partnerID=40&amp;md5=fe62933099f2553f60478e65194b7c41', event);\">\n    Current insights into chromatin structure organization.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ilatovskiy, A.; Lebedev, D.; Filatov, M.; Petukhov, M.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 54(4): 298-306.  2012.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&amp;partnerID=40&amp;md5=fe62933099f2553f60478e65194b7c41\"\n     onclick=\"log_download('ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&amp;partnerID=40&amp;md5=fe62933099f2553f60478e65194b7c41', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Current insights into chromatin structure organization [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ilatovskiy-lebedev-filatov-petukhov-isaevivanov-currentinsightsintochromatinstructureorganization-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ilatovskiy2012298,\r\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\r\ntitle={Current insights into chromatin structure organization},\r\njournal={Tsitologiya},\r\nyear={2012},\r\nvolume={54},\r\nnumber={4},\r\npages={298-306},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864496725&amp;partnerID=40&amp;md5=fe62933099f2553f60478e65194b7c41},\r\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},\r\nabstract={This review summarizes current insights into organization of chromatin structure at different levels of DNA compaction. Analysis of available experimental data allowed concluding that only nucleosomal level of structural organization was sufficiently investigated, whereas structure of a 30-nm chromatin fiber remains an open issue. The data on the chromatin structure obtained at the level of the nucleus speak in favor of a biphasic fractal organization of chromatin.},\r\nauthor_keywords={30-nm fiber;  Chromatin;  DNA;  Fractal;  Nucleosome},\r\ncorrespondence_address1={Ilatovskiy, A.V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: andrcyi@omrb.pnpi.spb.ru},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={22724366},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  This review summarizes current insights into organization of chromatin structure at different levels of DNA compaction. Analysis of available experimental data allowed concluding that only nucleosomal level of structural organization was sufficiently investigated, whereas structure of a 30-nm chromatin fiber remains an open issue. The data on the chromatin structure obtained at the level of the nucleus speak in favor of a biphasic fractal organization of chromatin.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&amp;partnerID=40&amp;md5=66c4733440532eaa83659d67c8673764\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&amp;partnerID=40&amp;md5=66c4733440532eaa83659d67c8673764', event);\">\n    Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Naiyzhny, S.; Landa, S.; Burdackov, V.; Artamonova, T.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 54(5): 430-438.  2012.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&amp;partnerID=40&amp;md5=66c4733440532eaa83659d67c8673764\"\n     onclick=\"log_download('shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&amp;partnerID=40&amp;md5=66c4733440532eaa83659d67c8673764', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-naiyzhny-landa-burdackov-artamonova-filatov-isolationandproteomicanalysisofexosomessecretedbyhumancancercellsinvitro-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2012430,\r\nauthor={Shtam, T.A. and Naiyzhny, S.N. and Landa, S.B. and Burdackov, V.S. and Artamonova, T.O. and Filatov, M.V.},\r\ntitle={Isolation and proteomic analysis of exosomes secreted by human cancer cells in vitro},\r\njournal={Tsitologiya},\r\nyear={2012},\r\nvolume={54},\r\nnumber={5},\r\npages={430-438},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864549288&amp;partnerID=40&amp;md5=66c4733440532eaa83659d67c8673764},\r\naffiliation={B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, Russian Federation},\r\nabstract={Exosomes are 20-100 nm membrane vesicles of endocytic origin secreted by most cell types in vitro and in vivo. Since exosomes contain both RNA (mRNA and microRNA) and proteins, which can be transferred to another cell, and be functional in that new environment, these vesicles may be involved in the communication between cells. The secretion of exosomes by tumor cells and their implication in the transport and propagation of infectious cargo suggest their participation in pathological situations. Our purpose here is to describe methods for the production, purification, and proteomic characterization of exosomes derived from human cancer cells in vitro. Based on exosomes&#x27; unique lipidic composition, we have developed the new approach to increase production of exosomes by cells in vitro. Secondly, we have developed quality control by laser correlation spectroscopy for exosomal assays based on the amount of MHC class I and CD63 molecules on their surface. At last, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used after 2D electrophoresis for the proteomic analysis of exosomes derived from cancer cell lines. This study describes the protein composition of brain tumor cell-derived exosomes in more detail.},\r\nauthor_keywords={Exosomes;  Laser correlation spectroscopy;  Proteomic analysis},\r\ncorrespondence_address1={Filatov, M.V.; B. P. Konstantinov St. Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil53@mail.ru},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={22827041},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exosomes are 20-100 nm membrane vesicles of endocytic origin secreted by most cell types in vitro and in vivo. Since exosomes contain both RNA (mRNA and microRNA) and proteins, which can be transferred to another cell, and be functional in that new environment, these vesicles may be involved in the communication between cells. The secretion of exosomes by tumor cells and their implication in the transport and propagation of infectious cargo suggest their participation in pathological situations. Our purpose here is to describe methods for the production, purification, and proteomic characterization of exosomes derived from human cancer cells in vitro. Based on exosomes' unique lipidic composition, we have developed the new approach to increase production of exosomes by cells in vitro. Secondly, we have developed quality control by laser correlation spectroscopy for exosomal assays based on the amount of MHC class I and CD63 molecules on their surface. At last, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was used after 2D electrophoresis for the proteomic analysis of exosomes derived from cancer cell lines. This study describes the protein composition of brain tumor cell-derived exosomes in more detail.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&amp;doi=10.1073%2fpnas.1217417109&amp;partnerID=40&amp;md5=893faf9b8234bde71083129c7163f3ff\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&amp;doi=10.1073%2fpnas.1217417109&amp;partnerID=40&amp;md5=893faf9b8234bde71083129c7163f3ff', event);\">\n    Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109).\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Borghi, N.; Sorokina, M.; Shcherbakova, O.; Weis, W.; Pruitt, B.; Nelson, W.;  and Dunn, A.\n</span>\n\n  \n\n</span>\n\n  <i>Proceedings of the National Academy of Sciences of the United States of America</i>, 109(46): 19034.  2012.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&amp;doi=10.1073%2fpnas.1217417109&amp;partnerID=40&amp;md5=893faf9b8234bde71083129c7163f3ff\"\n     onclick=\"log_download('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&amp;doi=10.1073%2fpnas.1217417109&amp;partnerID=40&amp;md5=893faf9b8234bde71083129c7163f3ff', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109) [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1073/pnas.1217417109\"\n     onclick=\"log_download('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012', 'http://doi.org/10.1073/pnas.1217417109', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-erratumecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretchproceedingsofthenationalacademyofsciencesoftheunitedstatesofamerica2012109311256812573doi101073pnas1204390109-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Borghi201219034,\r\nauthor={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},\r\ntitle={Erratum: E-cadherin is under constitutive actomyosingenerated tension that is increased at cell-cell contacts upon externally applied stretch (Proceedings of the National Academy of Sciences of the United States of America (2012) 109, 31 (12568-12573) DOI: 10.1073/pnas.1204390109)},\r\njournal={Proceedings of the National Academy of Sciences of the United States of America},\r\nyear={2012},\r\nvolume={109},\r\nnumber={46},\r\npages={19034},\r\ndoi={10.1073/pnas.1217417109},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869235058&amp;doi=10.1073%2fpnas.1217417109&amp;partnerID=40&amp;md5=893faf9b8234bde71083129c7163f3ff},\r\ncorrespondence_address1={Borghi, N.},\r\npublisher={National Academy of Sciences},\r\nissn={00278424},\r\ncoden={PNASA},\r\nlanguage={English},\r\nabbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},\r\ndocument_type={Erratum},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&amp;partnerID=40&amp;md5=551a1ed8267ba2c6cffd52472fbc6df1\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&amp;partnerID=40&amp;md5=551a1ed8267ba2c6cffd52472fbc6df1', event);\">\n    [Absorption of iodofolic acids by the cells of malignant tumors].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Soroka, N.; Filatov, M.; Korolev, V.; Bagiian, G.; Aplin, K.;  and Gridasov, G.\n</span>\n\n  \n\n</span>\n\n  <i>Bioorganicheskaia khimiia</i>, 38(6): 734-744.  2012.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&amp;partnerID=40&amp;md5=551a1ed8267ba2c6cffd52472fbc6df1\"\n     onclick=\"log_download('soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&amp;partnerID=40&amp;md5=551a1ed8267ba2c6cffd52472fbc6df1', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"[Absorption of iodofolic acids by the cells of malignant tumors]. [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('soroka-filatov-korolev-bagiian-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Soroka2012734,\r\nauthor={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiian, G.A. and Aplin, K.D. and Gridasov, G.G.},\r\ntitle={[Absorption of iodofolic acids by the cells of malignant tumors].},\r\njournal={Bioorganicheskaia khimiia},\r\nyear={2012},\r\nvolume={38},\r\nnumber={6},\r\npages={734-744},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878876945&amp;partnerID=40&amp;md5=551a1ed8267ba2c6cffd52472fbc6df1},\r\nabstract={The uptake of 125-iodine labeled 3&#x27; iodofolic acid (I*F) and 3&#x27; iodo, 5 formyl tetrahydrofolic acid (I*FT) by the cells HeLa, ECV, L-41, human glioma, and rat glioma was studied. Human Embrionic Lung Fibroblasts (HELF) were taken for comparison as healthy cells. It was shown for *IF that its long-term uptake by cells L-41 and ECV is hundreds oftimes higher than those of HELF cells. The short-term uptake phase was studied for *IFT uptake. The dissociation constant was determined for a complex formed by *IFT and an acceptor in the HeLa cells, which is supposed to cause concentrative uptake of *IFT in cells. The dissociation constants of this acceptor complexes with folic acid, 3&#x27; iodofolic acid and 3&#x27;,5&#x27;-diiodofolic acid were determined by competition with I*FT. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and rats with a sarcoma grafted on his thigh and glioma grafted into the brain was studied. As was shown there are large differences in the concentration of *IF and *IFT in the tumor and in the healthy tissue, *IF concentration in thigh muscle of healthy being 5 times lower than those in tumor grafted to the thigh, and *IFT concentration in healthy brain being 10 times lower than in brain tumor.},\r\ncorrespondence_address1={Soroka, N.V.},\r\nissn={01323423},\r\npubmed_id={23547477},\r\nlanguage={Russian},\r\nabbrev_source_title={Bioorg. Khim.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The uptake of 125-iodine labeled 3' iodofolic acid (I*F) and 3' iodo, 5 formyl tetrahydrofolic acid (I*FT) by the cells HeLa, ECV, L-41, human glioma, and rat glioma was studied. Human Embrionic Lung Fibroblasts (HELF) were taken for comparison as healthy cells. It was shown for *IF that its long-term uptake by cells L-41 and ECV is hundreds oftimes higher than those of HELF cells. The short-term uptake phase was studied for *IFT uptake. The dissociation constant was determined for a complex formed by *IFT and an acceptor in the HeLa cells, which is supposed to cause concentrative uptake of *IFT in cells. The dissociation constants of this acceptor complexes with folic acid, 3' iodofolic acid and 3',5'-diiodofolic acid were determined by competition with I*FT. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and rats with a sarcoma grafted on his thigh and glioma grafted into the brain was studied. As was shown there are large differences in the concentration of *IF and *IFT in the tumor and in the healthy tissue, *IF concentration in thigh muscle of healthy being 5 times lower than those in tumor grafted to the thigh, and *IFT concentration in healthy brain being 10 times lower than in brain tumor.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&amp;doi=10.1134%2fS1068162012060131&amp;partnerID=40&amp;md5=f27639cceea9ebc8bc5cd19271332c20\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&amp;doi=10.1134%2fS1068162012060131&amp;partnerID=40&amp;md5=f27639cceea9ebc8bc5cd19271332c20', event);\">\n    Absorption of iodofolic acids by the cells of malignant tumors.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Soroka, N.; Filatov, M.; Korolev, V.; Bagiyan, G.; Aplin, K.;  and Gridasov, G.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Bioorganic Chemistry</i>, 38(6): 652-661.  2012.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&amp;doi=10.1134%2fS1068162012060131&amp;partnerID=40&amp;md5=f27639cceea9ebc8bc5cd19271332c20\"\n     onclick=\"log_download('soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&amp;doi=10.1134%2fS1068162012060131&amp;partnerID=40&amp;md5=f27639cceea9ebc8bc5cd19271332c20', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Absorption of iodofolic acids by the cells of malignant tumors [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1068162012060131\"\n     onclick=\"log_download('soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012', 'http://doi.org/10.1134/S1068162012060131', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('soroka-filatov-korolev-bagiyan-aplin-gridasov-absorptionofiodofolicacidsbythecellsofmalignanttumors-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Soroka2012652,\r\nauthor={Soroka, N.V. and Filatov, M.V. and Korolev, V.G. and Bagiyan, G.A. and Aplin, K.D. and Gridasov, G.G.},\r\ntitle={Absorption of iodofolic acids by the cells of malignant tumors},\r\njournal={Russian Journal of Bioorganic Chemistry},\r\nyear={2012},\r\nvolume={38},\r\nnumber={6},\r\npages={652-661},\r\ndoi={10.1134/S1068162012060131},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870426049&amp;doi=10.1134%2fS1068162012060131&amp;partnerID=40&amp;md5=f27639cceea9ebc8bc5cd19271332c20},\r\naffiliation={Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; Khlopin Radium Institute, Russian Federation},\r\nabstract={The uptake of our synthesized 125-iodine labeled 3&#x27;-iodofolic acid ( *IF) and 3&#x27;-iodo-5-formyltetrahyrofolic acid ( *IFT) by the HeLa, EVC, L-41, human glioma, and rat glioma cells has been studied. The Human Embryonic Lung Fibroblasts (HELFs) were used as healthy control cells for comparison. It was shown for the quantity of *IF absorbed at its long-term uptake phase by the L-41 and ECV cells is hundreds of times higher than that of the HELF cells. The short-term *IFT uptake phase by the cells was studied. For the HeLa cells, the dissociation constant was determined for the *IFT complex with an acceptor, which is supposed to be responsible for *IFT accumulation in the cells. Using competitive sorption approach, the dissociation constants of folic, 3&#x27;-iodofolic, and 3&#x27;,5&#x27;-diiodofolic acid complexes with this acceptor were assessed. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and also in rats with sarcoma grafted on their thigh and glioma grafted into the brain was studied. A considerable differences were shown in the concentrations of *IF and *IFT in the tumor and in the healthy tissue, i.e., *IF in the healthy thigh muscle is five times lower than in the tumor grafted to the thigh, and *IFT concentration is 10 times lower in the healthy brain compared to the brain tumor. © Pleiades Publishing, Ltd., 2012.},\r\nauthor_keywords={Absorption;  Folates;  Iodine derivatives;  Isotope 125I;  Malignant cells},\r\ncorrespondence_address1={Filatov, M.V.; Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188350, Russian Federation; email: fil53ster@gmail.ru},\r\nissn={10681620},\r\ncoden={RJBCE},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Bioorg. Chem.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The uptake of our synthesized 125-iodine labeled 3'-iodofolic acid ( *IF) and 3'-iodo-5-formyltetrahyrofolic acid ( *IFT) by the HeLa, EVC, L-41, human glioma, and rat glioma cells has been studied. The Human Embryonic Lung Fibroblasts (HELFs) were used as healthy control cells for comparison. It was shown for the quantity of *IF absorbed at its long-term uptake phase by the L-41 and ECV cells is hundreds of times higher than that of the HELF cells. The short-term *IFT uptake phase by the cells was studied. For the HeLa cells, the dissociation constant was determined for the *IFT complex with an acceptor, which is supposed to be responsible for *IFT accumulation in the cells. Using competitive sorption approach, the dissociation constants of folic, 3'-iodofolic, and 3',5'-diiodofolic acid complexes with this acceptor were assessed. The distribution ratio of *IF and *IFT in tissues of different organs of healthy mice and rats and also in rats with sarcoma grafted on their thigh and glioma grafted into the brain was studied. A considerable differences were shown in the concentrations of *IF and *IFT in the tumor and in the healthy tissue, i.e., *IF in the healthy thigh muscle is five times lower than in the tumor grafted to the thigh, and *IFT concentration is 10 times lower in the healthy brain compared to the brain tumor. © Pleiades Publishing, Ltd., 2012.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&amp;doi=10.3732%2fajb.1100346&amp;partnerID=40&amp;md5=16a3d02c0039ad7f415ea0468134678f\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&amp;doi=10.3732%2fajb.1100346&amp;partnerID=40&amp;md5=16a3d02c0039ad7f415ea0468134678f', event);\">\n    Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae).\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Sun, X.; Yang, T.; Guan, J.; Ma, Y.; Jiang, J.; Cao, R.; Burlyaeva, M.; Vishnyakova, M.; Semenova, E.; Bulyntsev, S.;  and Zong, X.\n</span>\n\n  \n\n</span>\n\n  <i>American Journal of Botany</i>, 99(10): e379-e390.  2012.\n  <span class=\"note\">cited By 15</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&amp;doi=10.3732%2fajb.1100346&amp;partnerID=40&amp;md5=16a3d02c0039ad7f415ea0468134678f\"\n     onclick=\"log_download('sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&amp;doi=10.3732%2fajb.1100346&amp;partnerID=40&amp;md5=16a3d02c0039ad7f415ea0468134678f', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae) [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3732/ajb.1100346\"\n     onclick=\"log_download('sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012', 'http://doi.org/10.3732/ajb.1100346', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sun-yang-guan-ma-jiang-cao-burlyaeva-vishnyakova-etal-developmentof161novelestssrmarkersfromlathyrussativusfabaceae-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Sun2012,\r\nauthor={Sun, X.-L. and Yang, T. and Guan, J.-P. and Ma, Y. and Jiang, J.-Y. and Cao, R. and Burlyaeva, M. and Vishnyakova, M. and Semenova, E. and Bulyntsev, S. and Zong, X.-X.},\r\ntitle={Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae)},\r\njournal={American Journal of Botany},\r\nyear={2012},\r\nvolume={99},\r\nnumber={10},\r\npages={e379-e390},\r\ndoi={10.3732/ajb.1100346},\r\nnote={cited By 15},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867598270&amp;doi=10.3732%2fajb.1100346&amp;partnerID=40&amp;md5=16a3d02c0039ad7f415ea0468134678f},\r\naffiliation={Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Biological Sciences, China Agricultural University, Beijing 100193, China; Department of Leguminous Crops Genetic Resources, N. I. Vavilov Research Institute of Plant Industry, St. Petersburg 190000, Russian Federation},\r\nabstract={• Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. • Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. • Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea. © 2012 Botanical Society of America.},\r\nauthor_keywords={Expressed sequence tag;  Fabaceae;  Grasspea;  Lathyrus sativus;  Microsatellite;  Primer},\r\ncorrespondence_address1={Zong, X.-X.; Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; email: zongxx@mail.caas.net.cn},\r\nissn={00029122},\r\ncoden={AJBOA},\r\npubmed_id={23028003},\r\nlanguage={English},\r\nabbrev_source_title={Am. J. Bot.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  • Premise of the study: Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. • Methods and Results: Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. • Conclusions: These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea. © 2012 Botanical Society of America.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&amp;doi=10.1073%2fpnas.1204390109&amp;partnerID=40&amp;md5=27a25f91d046dadaaa32776ddecb5cfa\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&amp;doi=10.1073%2fpnas.1204390109&amp;partnerID=40&amp;md5=27a25f91d046dadaaa32776ddecb5cfa', event);\">\n    E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Borghi, N.; Sorokina, M.; Shcherbakova, O.; Weis, W.; Pruitt, B.; Nelson, W.;  and Dunn, A.\n</span>\n\n  \n\n</span>\n\n  <i>Proceedings of the National Academy of Sciences of the United States of America</i>, 109(31): 12568-12573.  2012.\n  <span class=\"note\">cited By 284</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&amp;doi=10.1073%2fpnas.1204390109&amp;partnerID=40&amp;md5=27a25f91d046dadaaa32776ddecb5cfa\"\n     onclick=\"log_download('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&amp;doi=10.1073%2fpnas.1204390109&amp;partnerID=40&amp;md5=27a25f91d046dadaaa32776ddecb5cfa', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1073/pnas.1204390109\"\n     onclick=\"log_download('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012', 'http://doi.org/10.1073/pnas.1204390109', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('borghi-sorokina-shcherbakova-weis-pruitt-nelson-dunn-ecadherinisunderconstitutiveactomyosingeneratedtensionthatisincreasedatcellcellcontactsuponexternallyappliedstretch-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Borghi201212568,\r\nauthor={Borghi, N. and Sorokina, M. and Shcherbakova, O.G. and Weis, W.I. and Pruitt, B.L. and Nelson, W.J. and Dunn, A.R.},\r\ntitle={E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch},\r\njournal={Proceedings of the National Academy of Sciences of the United States of America},\r\nyear={2012},\r\nvolume={109},\r\nnumber={31},\r\npages={12568-12573},\r\ndoi={10.1073/pnas.1204390109},\r\nnote={cited By 284},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864506988&amp;doi=10.1073%2fpnas.1204390109&amp;partnerID=40&amp;md5=27a25f91d046dadaaa32776ddecb5cfa},\r\naffiliation={Department of Biology, Stanford University, Stanford, CA 94305, United States; Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, United States; Department of Cell Biology, Centre National de la Recherche Scientifique, Université Paris-Diderot, Paris 75013, France},\r\nabstract={Classical cadherins are transmembrane proteins at the core of intercellular adhesion complexes in cohesive metazoan tissues. The extracellular domain of classical cadherins forms intercellular bonds with cadherins on neighboring cells, whereas the cytoplasmic domain recruits catenins, which in turn associate with additional cytoskeleton binding and regulatory proteins. Cadherin/catenin complexes are hypothesized to play a role in the transduction of mechanical forces that shape cells and tissues during development, regeneration, and disease. Whether mechanical forces are transduced directly through cadherins is unknown. To address this question, we used a Förster resonance energy transfer (FRET)-based molecular tension sensor to test the origin and magnitude of tensile forces transmitted through the cytoplasmic domain of E-cadherin in epithelial cells. We show that the actomyosin cytoskeleton exerts pN-tensile force on E-cadherin, and that this tension requires the catenin-binding domain of E-cadherin and αE-catenin. Surprisingly, the actomyosin cytoskeleton constitutively exerts tension on E-cadherin at the plasma membrane regardless of whether or not E-cadherin is recruited to cell-cell contacts, although tension is further increased at cell-cell contacts when adhering cells are stretched. Our findings thus point to a constitutive role of E-cadherin in transducing mechanical forces between the actomyosin cytoskeleton and the plasmamembrane, not only at cell-cell junctions but throughout the cell surface.},\r\nauthor_keywords={Mechanobiology;  Mechanosensor;  Mechanotransduction;  Morphogenesis;  Signal transduction},\r\ncorrespondence_address1={Dunn, A.R.; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, United States; email: alex.dunn@stanford.edu},\r\npublisher={National Academy of Sciences},\r\nissn={00278424},\r\ncoden={PNASA},\r\npubmed_id={22802638},\r\nlanguage={English},\r\nabbrev_source_title={Proc. Natl. Acad. Sci. U. S. A.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Classical cadherins are transmembrane proteins at the core of intercellular adhesion complexes in cohesive metazoan tissues. The extracellular domain of classical cadherins forms intercellular bonds with cadherins on neighboring cells, whereas the cytoplasmic domain recruits catenins, which in turn associate with additional cytoskeleton binding and regulatory proteins. Cadherin/catenin complexes are hypothesized to play a role in the transduction of mechanical forces that shape cells and tissues during development, regeneration, and disease. Whether mechanical forces are transduced directly through cadherins is unknown. To address this question, we used a Förster resonance energy transfer (FRET)-based molecular tension sensor to test the origin and magnitude of tensile forces transmitted through the cytoplasmic domain of E-cadherin in epithelial cells. We show that the actomyosin cytoskeleton exerts pN-tensile force on E-cadherin, and that this tension requires the catenin-binding domain of E-cadherin and αE-catenin. Surprisingly, the actomyosin cytoskeleton constitutively exerts tension on E-cadherin at the plasma membrane regardless of whether or not E-cadherin is recruited to cell-cell contacts, although tension is further increased at cell-cell contacts when adhering cells are stretched. Our findings thus point to a constitutive role of E-cadherin in transducing mechanical forces between the actomyosin cytoskeleton and the plasmamembrane, not only at cell-cell junctions but throughout the cell surface.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&amp;doi=10.1134%2fS1990519X12040116&amp;partnerID=40&amp;md5=621ea006f96d0bd38461fec3a60fde94\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&amp;doi=10.1134%2fS1990519X12040116&amp;partnerID=40&amp;md5=621ea006f96d0bd38461fec3a60fde94', event);\">\n    Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Naryzhny, S.; Landa, S.; Burdakov, V.; Artamonova, T.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 6(4): 317-325.  2012.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&amp;doi=10.1134%2fS1990519X12040116&amp;partnerID=40&amp;md5=621ea006f96d0bd38461fec3a60fde94\"\n     onclick=\"log_download('shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&amp;doi=10.1134%2fS1990519X12040116&amp;partnerID=40&amp;md5=621ea006f96d0bd38461fec3a60fde94', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X12040116\"\n     onclick=\"log_download('shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012', 'http://doi.org/10.1134/S1990519X12040116', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-naryzhny-landa-burdakov-artamonova-filatov-purificationandinvitroanalysisofexosomessecretedbymalignantlytransformedhumancells-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2012317,\r\nauthor={Shtam, T.A. and Naryzhny, S.N. and Landa, S.B. and Burdakov, V.S. and Artamonova, T.O. and Filatov, M.V.},\r\ntitle={Purification and in vitro analysis of exosomes secreted by malignantly transformed human cells},\r\njournal={Cell and Tissue Biology},\r\nyear={2012},\r\nvolume={6},\r\nnumber={4},\r\npages={317-325},\r\ndoi={10.1134/S1990519X12040116},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865567518&amp;doi=10.1134%2fS1990519X12040116&amp;partnerID=40&amp;md5=621ea006f96d0bd38461fec3a60fde94},\r\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\r\nabstract={Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that quantitative and qualitative characterization of these microparticles produced by different tissues in normal and pathological states can give valuable diagnostic and prognostic information and be a biomarker of different diseases, including oncological ones. Elaboration of the purification of exosomes and their proteome analysis was the aim of the present work. An original approach to enhancing exosome production in cultured transformed human cells was developed. The data obtained allowed us to detect exosomes in cultural conditioned samples and control the quality of produced exosomes at all stages of their purification. Electrophoretic analysis of proteins obtained from exosomes of different origins shows differences in protein profiles. Proteins from exosomes of glioblastoma cell lines were separated by two-dimensional electrophoresis. Protein profiles were further analyzed by densitometry and mass spectrometry, which allowed more than 30 proteins, including specific tumor markers, to be identified. © 2012 Pleiades Publishing, Ltd.},\r\nauthor_keywords={exosomes;  laser correlation spectroscopy;  proteome analysis},\r\ncorrespondence_address1={Filatov, M. V.; Petersburg Nuclear Physics Institute, Gatchina, Russian Federation; email: fil_53@mail.ru},\r\nissn={1990519X},\r\nlanguage={English},\r\nabbrev_source_title={Cell Tissue Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exosomes are natural nanoparticles secreted by different cells and capable of carrying protein markers and genetic information, thus participating in cellular communication. There is good reason to think that quantitative and qualitative characterization of these microparticles produced by different tissues in normal and pathological states can give valuable diagnostic and prognostic information and be a biomarker of different diseases, including oncological ones. Elaboration of the purification of exosomes and their proteome analysis was the aim of the present work. An original approach to enhancing exosome production in cultured transformed human cells was developed. The data obtained allowed us to detect exosomes in cultural conditioned samples and control the quality of produced exosomes at all stages of their purification. Electrophoretic analysis of proteins obtained from exosomes of different origins shows differences in protein profiles. Proteins from exosomes of glioblastoma cell lines were separated by two-dimensional electrophoresis. Protein profiles were further analyzed by densitometry and mass spectrometry, which allowed more than 30 proteins, including specific tumor markers, to be identified. © 2012 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&amp;doi=10.1109%2fMEMSYS.2012.6170320&amp;partnerID=40&amp;md5=10bc083a2824dc630c10ffed9ff11ea6\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&amp;doi=10.1109%2fMEMSYS.2012.6170320&amp;partnerID=40&amp;md5=10bc083a2824dc630c10ffed9ff11ea6', event);\">\n    Uniaxial cell stretcher enables high resolution live cell imaging.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Sim, J.; Borghi, N.; Ribeiro, A.; Sorokina, M.; Shcherbakova, O.; Ramallo, D.; Dunn, A.; Nelson, W.;  and Pruitt, B.\n</span>\n\n  \n\n</span>\n\n   2012.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 2; Conference of 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference Date: 29 January 2012 Through 2 February 2012; Conference Code:89445</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&amp;doi=10.1109%2fMEMSYS.2012.6170320&amp;partnerID=40&amp;md5=10bc083a2824dc630c10ffed9ff11ea6\"\n     onclick=\"log_download('sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&amp;doi=10.1109%2fMEMSYS.2012.6170320&amp;partnerID=40&amp;md5=10bc083a2824dc630c10ffed9ff11ea6', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Uniaxial cell stretcher enables high resolution live cell imaging [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1109/MEMSYS.2012.6170320\"\n     onclick=\"log_download('sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012', 'http://doi.org/10.1109/MEMSYS.2012.6170320', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sim-borghi-ribeiro-sorokina-shcherbakova-ramallo-dunn-nelson-etal-uniaxialcellstretcherenableshighresolutionlivecellimaging-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Sim2012854,\r\nauthor={Sim, J.Y. and Borghi, N. and Ribeiro, A. and Sorokina, M. and Shcherbakova, O. and Ramallo, D. and Dunn, A. and Nelson, W.J. and Pruitt, B.L.},\r\ntitle={Uniaxial cell stretcher enables high resolution live cell imaging},\r\njournal={Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)},\r\nyear={2012},\r\npages={854-857},\r\ndoi={10.1109/MEMSYS.2012.6170320},\r\nart_number={6170320},\r\nnote={cited By 2; Conference of 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference Date: 29 January 2012 Through 2 February 2012;  Conference Code:89445},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860464996&amp;doi=10.1109%2fMEMSYS.2012.6170320&amp;partnerID=40&amp;md5=10bc083a2824dc630c10ffed9ff11ea6},\r\naffiliation={Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; Biology, Stanford University, CA, United States; Chemical Engineering, Stanford University, CA, United States},\r\nabstract={Imaging adherent cells cultured on commercially available stretchable substrates presents challenges for high-magnification objectives. Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson&#x27;s contraction with stretching, and issues of optical transparency or non-matching refractive indices. Beyond the advantages of visualizing biological structures in detail, we seek to implement specialized high-resolution fluorescence microscopy techniques such as Förster Resonance Energy Transfer (FRET) microscopy while stretching. Enabling FRET imaging of stretched cells provides a powerful tool for modern cell biology and mechanobiology [1]. FRET techniques require high magnification as well as a stable focal plane for imaging. Here, we address this requirement for stretchable substrates with a microfabricated cell strain device suitable for live cell imaging while allowing high-resolution FRET microscopy of cells. We adapt a uniaxial cell stretching concept previously demonstrated by Huh [2] for high magnification imaging by using thin bottom channels and membranes supported above an inverted oil immersion objective. This work presents a major advance for research in cell mechanobiology as it enables direct mechanical actuation combined with imaging of FRET probes engineered to report strained proteins in live cells. © 2012 IEEE.},\r\ncorrespondence_address1={Sim, J.Y.; Department of Mechanical Engineering, Stanford University, 418 Panama Mall, Stanford, CA 94305, United States; email: simba85@stanford.edu},\r\nsponsors={Robotics and Automation Society; IEEE; Region Nord-Pas de Calais},\r\naddress={Paris},\r\nissn={10846999},\r\nisbn={9781467303248},\r\ncoden={PMEME},\r\nlanguage={English},\r\nabbrev_source_title={Proc. IEEE Int. Conf. Micro Electro Mech. Syst. MEMS},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Imaging adherent cells cultured on commercially available stretchable substrates presents challenges for high-magnification objectives. Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson's contraction with stretching, and issues of optical transparency or non-matching refractive indices. Beyond the advantages of visualizing biological structures in detail, we seek to implement specialized high-resolution fluorescence microscopy techniques such as Förster Resonance Energy Transfer (FRET) microscopy while stretching. Enabling FRET imaging of stretched cells provides a powerful tool for modern cell biology and mechanobiology [1]. FRET techniques require high magnification as well as a stable focal plane for imaging. Here, we address this requirement for stretchable substrates with a microfabricated cell strain device suitable for live cell imaging while allowing high-resolution FRET microscopy of cells. We adapt a uniaxial cell stretching concept previously demonstrated by Huh [2] for high magnification imaging by using thin bottom channels and membranes supported above an inverted oil immersion objective. This work presents a major advance for research in cell mechanobiology as it enables direct mechanical actuation combined with imaging of FRET probes engineered to report strained proteins in live cells. © 2012 IEEE.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&amp;doi=10.1371%2fjournal.pone.0033920&amp;partnerID=40&amp;md5=615d0f3eb9de5faa02e836da1c904c89\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&amp;doi=10.1371%2fjournal.pone.0033920&amp;partnerID=40&amp;md5=615d0f3eb9de5faa02e836da1c904c89', event);\">\n    Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Bruder, J.; Semenova, E.; Chen, P.; Limbach, K.; Patterson, N.; Stefaniak, M.; Konovalova, S.; Thomas, C.; Hamilton, M.; King, C.; Richie, T.;  and Doolan, D.\n</span>\n\n  \n\n</span>\n\n  <i>PLoS ONE</i>, 7(4).  2012.\n  <span class=\"note\">cited By 24</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&amp;doi=10.1371%2fjournal.pone.0033920&amp;partnerID=40&amp;md5=615d0f3eb9de5faa02e836da1c904c89\"\n     onclick=\"log_download('bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&amp;doi=10.1371%2fjournal.pone.0033920&amp;partnerID=40&amp;md5=615d0f3eb9de5faa02e836da1c904c89', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1371/journal.pone.0033920\"\n     onclick=\"log_download('bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012', 'http://doi.org/10.1371/journal.pone.0033920', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('bruder-semenova-chen-limbach-patterson-stefaniak-konovalova-thomas-etal-modificationofad5hexonhypervariableregionscircumventspreexistingad5neutralizingantibodiesandinducesprotectiveimmuneresponses-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Bruder2012,\r\nauthor={Bruder, J.T. and Semenova, E. and Chen, P. and Limbach, K. and Patterson, N.B. and Stefaniak, M.E. and Konovalova, S. and Thomas, C. and Hamilton, M. and King, C.R. and Richie, T.L. and Doolan, D.L.},\r\ntitle={Modification of Ad5 hexon hypervariable regions circumvents pre-existing Ad5 neutralizing antibodies and induces protective immune responses},\r\njournal={PLoS ONE},\r\nyear={2012},\r\nvolume={7},\r\nnumber={4},\r\ndoi={10.1371/journal.pone.0033920},\r\nart_number={e33920},\r\nnote={cited By 24},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859345005&amp;doi=10.1371%2fjournal.pone.0033920&amp;partnerID=40&amp;md5=615d0f3eb9de5faa02e836da1c904c89},\r\naffiliation={Research, GenVec, Inc., Gaithersburg, MD, United States; US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, MD, United States; Queensland Institute of Medical Research, Brisbane, Australia; University of California San Diego, La Jolla, CA, United States; MedImmune, Inc., Gaithersburg, MD, United States; International Aids Vaccine Initiative, New York, NY, United States},\r\nabstract={The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.},\r\ncorrespondence_address1={Bruder, J. T.; Research, GenVec, Inc., Gaithersburg, MD, United States; email: jbruder@genvec.com},\r\nissn={19326203},\r\npubmed_id={22496772},\r\nlanguage={English},\r\nabbrev_source_title={PLoS ONE},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The development of an effective malaria vaccine is a high global health priority. Vaccine vectors based on adenovirus type 5 are capable of generating robust and protective T cell and antibody responses in animal models and are currently being evaluated in clinical trials for HIV and malaria. They appear to be more effective in terms of inducing antigen-specific immune responses as compared with non-Ad5 serotype vectors. However, the high prevalence of neutralizing antibodies to Ad5 in the human population, particularly in the developing world, has the potential to limit the effectiveness of Ad5-based vaccines. We have generated novel Ad5-based vectors that precisely replace the hexon hypervariable regions with those derived from Ad43, a subgroup D serotype with low prevalence of neutralizing antibody in humans. We have demonstrated that these hexon-modified adenovectors are not neutralized efficiently by Ad5 neutralizing antibodies in vitro using sera from mice, rabbits and human volunteers. We have also generated hexon-modified adenovectors that express a rodent malaria parasite antigen, PyCSP, and demonstrated that they are as immunogenic as an unmodified vector. Furthermore, in contrast to the unmodified vector, the hexon-modified adenovectors induced robust T cell responses in mice with high levels of Ad5 neutralizing antibody. We also show that the hexon-modified vector can be combined with unmodified Ad5 vector in prime-boost regimens to induce protective responses in mice. Our data establish that these hexon-modified vectors are highly immunogenic even in the presence of pre-existing anti-adenovirus antibodies. These hexon-modified adenovectors may have advantages in sub-Saharan Africa where there is a high prevalence of Ad5 neutralizing antibody in the population.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&amp;doi=10.1088%2f1742-6596%2f351%2f1%2f012007&amp;partnerID=40&amp;md5=4cb28476bafca2cc34da1a137dfb05ea\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&amp;doi=10.1088%2f1742-6596%2f351%2f1%2f012007&amp;partnerID=40&amp;md5=4cb28476bafca2cc34da1a137dfb05ea', event);\">\n    SANS spectra of the fractal supernucleosomal chromatin structure models.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ilatovskiy, A.; Lebedev, D.; Filatov, M.; Petukhov, M.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n   2012.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 7; Conference of 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich's Birth ; Conference Date: 27 May 2011 Through 30 May 2011; Conference Code:89603</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&amp;doi=10.1088%2f1742-6596%2f351%2f1%2f012007&amp;partnerID=40&amp;md5=4cb28476bafca2cc34da1a137dfb05ea\"\n     onclick=\"log_download('ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&amp;doi=10.1088%2f1742-6596%2f351%2f1%2f012007&amp;partnerID=40&amp;md5=4cb28476bafca2cc34da1a137dfb05ea', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"SANS spectra of the fractal supernucleosomal chromatin structure models [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1088/1742-6596/351/1/012007\"\n     onclick=\"log_download('ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012', 'http://doi.org/10.1088/1742-6596/351/1/012007', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ilatovskiy-lebedev-filatov-petukhov-isaevivanov-sansspectraofthefractalsupernucleosomalchromatinstructuremodels-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Ilatovskiy2012,\r\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\r\ntitle={SANS spectra of the fractal supernucleosomal chromatin structure models},\r\njournal={Journal of Physics: Conference Series},\r\nyear={2012},\r\nvolume={351},\r\nnumber={1},\r\ndoi={10.1088/1742-6596/351/1/012007},\r\nart_number={012007},\r\nnote={cited By 7; Conference of 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich&#x27;s Birth ; Conference Date: 27 May 2011 Through 30 May 2011;  Conference Code:89603},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860655272&amp;doi=10.1088%2f1742-6596%2f351%2f1%2f012007&amp;partnerID=40&amp;md5=4cb28476bafca2cc34da1a137dfb05ea},\r\naffiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnic University, St. Petersburg, Russian Federation},\r\nabstract={The eukaryotic genome consists of chromatin - a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions - 3 and 2. © Published under licence by IOP Publishing Ltd.},\r\ncorrespondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},\r\nsponsors={Comenius University in Bratislava; Horia Hulubei Natl. Inst. Phys. Nucl. Eng. (IFIN HH); Institute of Macromolecular Chemistry AS CR; Jt. Inst. Nucl. Res., Frank Lab. Neutron Phys.},\r\npublisher={Institute of Physics Publishing},\r\naddress={Dubna},\r\nissn={17426588},\r\nlanguage={English},\r\nabbrev_source_title={J. Phys. Conf. Ser.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The eukaryotic genome consists of chromatin - a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions - 3 and 2. © Published under licence by IOP Publishing Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&amp;doi=10.1002%2fmds.23901&amp;partnerID=40&amp;md5=6dc7221fa668d60863daa976f09cf055\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&amp;doi=10.1002%2fmds.23901&amp;partnerID=40&amp;md5=6dc7221fa668d60863daa976f09cf055', event);\">\n    Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson's disease.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Shadrina, M.; Slominsky, P.; Ivanova-Smolenskaya, I.; Bagyeva, G.; Illarioshkin, S.;  and Limborska, S.\n</span>\n\n  \n\n</span>\n\n  <i>Movement Disorders</i>, 27(1): 139-143.  2012.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&amp;doi=10.1002%2fmds.23901&amp;partnerID=40&amp;md5=6dc7221fa668d60863daa976f09cf055\"\n     onclick=\"log_download('semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&amp;doi=10.1002%2fmds.23901&amp;partnerID=40&amp;md5=6dc7221fa668d60863daa976f09cf055', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson&#x27;s disease [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/mds.23901\"\n     onclick=\"log_download('semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012', 'http://doi.org/10.1002/mds.23901', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-shadrina-slominsky-ivanovasmolenskaya-bagyeva-illarioshkin-limborska-analysisofpark2geneexonrearrangementsinrussianpatientswithsporadicparkinsonsdisease-2012')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2012139,\r\nauthor={Semenova, E.V. and Shadrina, M.I. and Slominsky, P.A. and Ivanova-Smolenskaya, I.A. and Bagyeva, G. and Illarioshkin, S.N. and Limborska, S.A.},\r\ntitle={Analysis of PARK2 gene exon rearrangements in Russian patients with sporadic Parkinson&#x27;s disease},\r\njournal={Movement Disorders},\r\nyear={2012},\r\nvolume={27},\r\nnumber={1},\r\npages={139-143},\r\ndoi={10.1002/mds.23901},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855973029&amp;doi=10.1002%2fmds.23901&amp;partnerID=40&amp;md5=6dc7221fa668d60863daa976f09cf055},\r\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation; Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russian Federation},\r\nabstract={Background:: Deletions and duplications of single exons or exon groups account for a large proportion of the PARK2 gene mutations described in juvenile autosomal recessive Parkinson&#x27;s disease (PD). Methods:: We analyzed rearrangements in exons 1 to 12 of the PARK2 gene in Russian sporadic patients with early-onset PD (EOPD) and late-onset PD (LOPD). Results:: The frequency of EOPD and LOPD patients carrying these mutations was 12.4% and 3.8%, respectively. The most frequent rearrangements were detected in exons 3 and 4. The odds ratio for EOPD in individuals carrying PARK2 exon deletions and duplications was 13.95 (95% confidence interval [CI], 1.846-105.46; P = .0022). In addition, we found a correlation between exon rearrangements in PARK2 and the age at onset of PD, presence of dystonia, and symmetrical course of the disease. Conclusions:: Exon rearrangements in the PARK2 gene play a significant role in the pathogenesis of sporadic PD in Russian patients. © 2011 Movement Disorder Society.},\r\nauthor_keywords={Deletion;  Duplication;  PARK2 exon rearrangements;  Parkinson&#x27;s disease},\r\ncorrespondence_address1={Illarioshkin, S.N.Volokolamskoe Shosse 80, Moscow 125367, Russian Federation; email: snillario@gmail.com},\r\nissn={08853185},\r\ncoden={MOVDE},\r\npubmed_id={21915905},\r\nlanguage={English},\r\nabbrev_source_title={Mov. Disord.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Background:: Deletions and duplications of single exons or exon groups account for a large proportion of the PARK2 gene mutations described in juvenile autosomal recessive Parkinson's disease (PD). Methods:: We analyzed rearrangements in exons 1 to 12 of the PARK2 gene in Russian sporadic patients with early-onset PD (EOPD) and late-onset PD (LOPD). Results:: The frequency of EOPD and LOPD patients carrying these mutations was 12.4% and 3.8%, respectively. The most frequent rearrangements were detected in exons 3 and 4. The odds ratio for EOPD in individuals carrying PARK2 exon deletions and duplications was 13.95 (95% confidence interval [CI], 1.846-105.46; P = .0022). In addition, we found a correlation between exon rearrangements in PARK2 and the age at onset of PD, presence of dystonia, and symmetrical course of the disease. Conclusions:: Exon rearrangements in the PARK2 gene play a significant role in the pathogenesis of sporadic PD in Russian patients. © 2011 Movement Disorder Society.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2011\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2011')\"\n      onkeypress=\"toggleGroup('group_2011')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2011</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&amp;doi=10.1063%2f1.3661987&amp;partnerID=40&amp;md5=8a4868aeeffdd6637dac55d1e4fa84a9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&amp;doi=10.1063%2f1.3661987&amp;partnerID=40&amp;md5=8a4868aeeffdd6637dac55d1e4fa84a9', event);\">\n    Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ilatovskiy, A.; Lebedev, D.; Filatov, M.; Grigoriev, M.; Petukhov, M.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Journal of Applied Physics</i>, 110(10).  2011.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&amp;doi=10.1063%2f1.3661987&amp;partnerID=40&amp;md5=8a4868aeeffdd6637dac55d1e4fa84a9\"\n     onclick=\"log_download('ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&amp;doi=10.1063%2f1.3661987&amp;partnerID=40&amp;md5=8a4868aeeffdd6637dac55d1e4fa84a9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1063/1.3661987\"\n     onclick=\"log_download('ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011', 'http://doi.org/10.1063/1.3661987', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ilatovskiy-lebedev-filatov-grigoriev-petukhov-isaevivanov-modelingandsmallangleneutronscatteringspectraofchromatinsupernucleosomalstructuresatgenomescale-2011')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ilatovskiy2011,\r\nauthor={Ilatovskiy, A.V. and Lebedev, D.V. and Filatov, M.V. and Grigoriev, M. and Petukhov, M.G. and Isaev-Ivanov, V.V.},\r\ntitle={Modeling and small-angle neutron scattering spectra of chromatin supernucleosomal structures at genome scale},\r\njournal={Journal of Applied Physics},\r\nyear={2011},\r\nvolume={110},\r\nnumber={10},\r\ndoi={10.1063/1.3661987},\r\nart_number={102217},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555167434&amp;doi=10.1063%2f1.3661987&amp;partnerID=40&amp;md5=8a4868aeeffdd6637dac55d1e4fa84a9},\r\naffiliation={Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; Research and Education Center Biophysics, PNPI RAS, St. Petersburg State Polytechnical University, St. Petersburg, Russian Federation; Laboratoire Biologie Moléculaire Eukaryote, CNRS-Université Paul-Sabathier, Toulouse, France},\r\nabstract={Eukaryotic genome is a highly compacted nucleoprotein complex organized in a hierarchical structure based on nucleosomes. Detailed organization of this structure remains unknown. In the present work we developed algorithms for geometry modeling of the supernucleosomal chromatin structure and for computing distance distribution functions and small-angle neutron scattering (SANS) spectra of the genome-scale (∼106 nucleosomes) chromatin structure at residue resolution. Our physical nucleosome model was based on the mononucleosome crystal structure. A nucleosome was assumed to be rigid within a local coordinate system. Interface parameters between nucleosomes can be set for each nucleosome independently. Pair distance distributions were computed with Monte Carlo simulation. SANS spectra were calculated with Fourier transformation of weighted distance distribution; the concentration of heavy water in solvent and probability of H/D exchange were taken into account. Two main modes of supernucleosomal structure generation were used. In a free generation mode all interface parameters were chosen randomly, whereas nucleosome self-intersections were not allowed. The second generation mode (generation in volume) enabled spherical or cubical wall restrictions. It was shown that calculated SANS spectra for a number of our models were in general agreement with available experimental data. © 2011 American Institute of Physics.},\r\ncorrespondence_address1={Ilatovskiy, A.V.; Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Russian Federation; email: andreyi@omrb.pnpi.spb.ru},\r\nissn={00218979},\r\ncoden={JAPIA},\r\nlanguage={English},\r\nabbrev_source_title={J Appl Phys},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Eukaryotic genome is a highly compacted nucleoprotein complex organized in a hierarchical structure based on nucleosomes. Detailed organization of this structure remains unknown. In the present work we developed algorithms for geometry modeling of the supernucleosomal chromatin structure and for computing distance distribution functions and small-angle neutron scattering (SANS) spectra of the genome-scale (∼106 nucleosomes) chromatin structure at residue resolution. Our physical nucleosome model was based on the mononucleosome crystal structure. A nucleosome was assumed to be rigid within a local coordinate system. Interface parameters between nucleosomes can be set for each nucleosome independently. Pair distance distributions were computed with Monte Carlo simulation. SANS spectra were calculated with Fourier transformation of weighted distance distribution; the concentration of heavy water in solvent and probability of H/D exchange were taken into account. Two main modes of supernucleosomal structure generation were used. In a free generation mode all interface parameters were chosen randomly, whereas nucleosome self-intersections were not allowed. The second generation mode (generation in volume) enabled spherical or cubical wall restrictions. It was shown that calculated SANS spectra for a number of our models were in general agreement with available experimental data. © 2011 American Institute of Physics.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&amp;doi=10.1134%2fS1062360411010097&amp;partnerID=40&amp;md5=925eb3cf7929f0e3f073ac255b316768\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&amp;doi=10.1134%2fS1062360411010097&amp;partnerID=40&amp;md5=925eb3cf7929f0e3f073ac255b316768', event);\">\n    Study of chromatin decondensation factors in human spermatozoids by flow cytometry.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Developmental Biology</i>, 42(1): 16-24.  2011.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&amp;doi=10.1134%2fS1062360411010097&amp;partnerID=40&amp;md5=925eb3cf7929f0e3f073ac255b316768\"\n     onclick=\"log_download('semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&amp;doi=10.1134%2fS1062360411010097&amp;partnerID=40&amp;md5=925eb3cf7929f0e3f073ac255b316768', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Study of chromatin decondensation factors in human spermatozoids by flow cytometry [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1062360411010097\"\n     onclick=\"log_download('semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011', 'http://doi.org/10.1134/S1062360411010097', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova201116,\r\nauthor={Semenova, E.V. and Filatov, M.V.},\r\ntitle={Study of chromatin decondensation factors in human spermatozoids by flow cytometry},\r\njournal={Russian Journal of Developmental Biology},\r\nyear={2011},\r\nvolume={42},\r\nnumber={1},\r\npages={16-24},\r\ndoi={10.1134/S1062360411010097},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951526241&amp;doi=10.1134%2fS1062360411010097&amp;partnerID=40&amp;md5=925eb3cf7929f0e3f073ac255b316768},\r\naffiliation={Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation},\r\nabstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm. © 2011 Pleiades Publishing, Ltd.},\r\nauthor_keywords={endogenous nuclear proteases;  fertilization;  flow cytometry;  human spermatozoids;  protease inhibitors},\r\ncorrespondence_address1={Filatov, M. V.; Division of Molecular and Radiation Biophysics, Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad oblast, Orlova Roshcha 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\r\nissn={10623604},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Dev. Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm. © 2011 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&amp;partnerID=40&amp;md5=e4b4997f53d5d4069bc4d51f710afbb4\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&amp;partnerID=40&amp;md5=e4b4997f53d5d4069bc4d51f710afbb4', event);\">\n    [Study of chromatin decondensation factors in human spermatozoids by flow cytometry].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Ontogenez</i>, 42(1): 20-29.  2011.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&amp;partnerID=40&amp;md5=e4b4997f53d5d4069bc4d51f710afbb4\"\n     onclick=\"log_download('semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&amp;partnerID=40&amp;md5=e4b4997f53d5d4069bc4d51f710afbb4', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"[Study of chromatin decondensation factors in human spermatozoids by flow cytometry]. [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-filatov-studyofchromatindecondensationfactorsinhumanspermatozoidsbyflowcytometry-2011')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova201120,\r\nauthor={Semenova, E.V. and Filatov, M.V.},\r\ntitle={[Study of chromatin decondensation factors in human spermatozoids by flow cytometry].},\r\njournal={Ontogenez},\r\nyear={2011},\r\nvolume={42},\r\nnumber={1},\r\npages={20-29},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79954541360&amp;partnerID=40&amp;md5=e4b4997f53d5d4069bc4d51f710afbb4},\r\nabstract={To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm.},\r\ncorrespondence_address1={Semenova, E.V.},\r\nissn={04751450},\r\npubmed_id={21442899},\r\nlanguage={Russian},\r\nabbrev_source_title={Ontogenez},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  To date, the mechanisms responsible for radical change of chromatin structure in male germ cells during fertilization are unclear. Evidence suggesting the existence of proteolytic nuclear enzymes in mature human spermatozoids are presented in this work. The possible role of these previously unknown proteases in decondensation of chromatin of spermatozoids in a fertilized ovum is discussed. Application of the flow cytometry technique has shown that treatment of human spermatozoid nuclei with SH-reagents leads not only to destruction of disulfide bonds between protamine molecules that is necessary for their effective utilization but also induces specific endogenous proteolytic activity that consequently results in rather fast decondensation of chromatin followed by proteolytic cleavage of nuclear proteins. A chromatin decondensation process can be almost totally blocked by serine protease inhibitors and components of seminal fluid. An original cytochemical approach of binding of fluorescently labeled protease inhibitor to the target of investigation has been used in order to visualize the localization of proteases in male germ cell nuclei. The results of our study suggest that one of the factors of chromatin reorganization involved in the formation of male pronucleus is endogenous nuclear protease of spermatozoids, which is activated by glutathione or other SH-components of ovum cytoplasm.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2010\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2010')\"\n      onkeypress=\"toggleGroup('group_2010')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2010</span>\n      <span class=\"bibbase_group_count\">\n        \n        (5)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&amp;partnerID=40&amp;md5=c3b287531eda58217f247310540fc220\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&amp;partnerID=40&amp;md5=c3b287531eda58217f247310540fc220', event);\">\n    Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Landa, S.; Pantina, R.;  and Garmai, Y.\n</span>\n\n  \n\n</span>\n\n  <i>Klinichescheskaya Laboratornaya Diagnostika</i>, (12): 35-43.  2010.\n  <span class=\"note\">cited By 8</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&amp;partnerID=40&amp;md5=c3b287531eda58217f247310540fc220\"\n     onclick=\"log_download('filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&amp;partnerID=40&amp;md5=c3b287531eda58217f247310540fc220', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-landa-pantina-garmai-investigationofexosomessecretedbydifferentnormalandmalignantcellsinvitroandinvivo-2010')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov201035,\r\nauthor={Filatov, M.V. and Landa, S.B. and Pantina, R.A. and Garmai, Yu.P.},\r\ntitle={Investigation of exosomes secreted by different normal and malignant cells in vitro and in vivo},\r\njournal={Klinichescheskaya Laboratornaya Diagnostika},\r\nyear={2010},\r\nnumber={12},\r\npages={35-43},\r\nnote={cited By 8},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951986882&amp;partnerID=40&amp;md5=c3b287531eda58217f247310540fc220},\r\nabstract={Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection ofexosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked.},\r\nauthor_keywords={Dynamic light scattering technique;  Exosomes;  Fibroblasts;  Lymphocytes},\r\ncorrespondence_address1={Filatov, M. V.email: filatov@omrb.pnpi.spb.ru},\r\nissn={08692084},\r\npubmed_id={21395053},\r\nlanguage={Russian},\r\nabbrev_source_title={Klin. Lab. Diagn.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Laser correlation spectroscopy, atomic force microscopy, and immunoaffinity chromatography were used to characterize exosomes produced by different human cells. The exosomes secreted into a culture medium by normal fibroblasts, dendritic cells, lymphocytes, as well as malignant cells obtained from tumors of various tissue origins. The similar investigations were made for exosomes detectable in plasma and cerebrospinal fluid. The dynamic light scattering technique has demonstrated that the exosomes from different sources are homogenous and similar in size of the order of 20 and 90 nm. The exceptional homogeneity of exosomes was confirmed by atomic force microscopy. The immunoaffinity method has shown that all the exosomes under study carry antigenic determinants recognizable by antibodies to the major histocompatibility complex of type 1 (HLA-ABC). A method is proposed for evidence-based detection ofexosomes in various biological fluids. For this, dynamic light scattering detects 20- and 90-nm particles and whether they can be removed by immunoaffinity chromatography with HLA-ABC antibodies is checked.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&amp;doi=10.1007%2fs10517-010-0976-2&amp;partnerID=40&amp;md5=283fbb036945227dc1e8fc31f3e078a3\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&amp;doi=10.1007%2fs10517-010-0976-2&amp;partnerID=40&amp;md5=283fbb036945227dc1e8fc31f3e078a3', event);\">\n    Detection of inflammatory processes during various diseases by the method of flow cytofluorometry.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Varfolomeeva, E.; Ivanov, E.; Drobchenko, E.; Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Bulletin of Experimental Biology and Medicine</i>, 149(4): 485-489.  2010.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&amp;doi=10.1007%2fs10517-010-0976-2&amp;partnerID=40&amp;md5=283fbb036945227dc1e8fc31f3e078a3\"\n     onclick=\"log_download('varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&amp;doi=10.1007%2fs10517-010-0976-2&amp;partnerID=40&amp;md5=283fbb036945227dc1e8fc31f3e078a3', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Detection of inflammatory processes during various diseases by the method of flow cytofluorometry [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/s10517-010-0976-2\"\n     onclick=\"log_download('varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010', 'http://doi.org/10.1007/s10517-010-0976-2', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('varfolomeeva-ivanov-drobchenko-semenova-filatov-detectionofinflammatoryprocessesduringvariousdiseasesbythemethodofflowcytofluorometry-2010')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Varfolomeeva2010485,\r\nauthor={Varfolomeeva, E.Y. and Ivanov, E.I. and Drobchenko, E.A. and Semenova, E.V. and Filatov, M.V.},\r\ntitle={Detection of inflammatory processes during various diseases by the method of flow cytofluorometry},\r\njournal={Bulletin of Experimental Biology and Medicine},\r\nyear={2010},\r\nvolume={149},\r\nnumber={4},\r\npages={485-489},\r\ndoi={10.1007/s10517-010-0976-2},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957857555&amp;doi=10.1007%2fs10517-010-0976-2&amp;partnerID=40&amp;md5=283fbb036945227dc1e8fc31f3e078a3},\r\naffiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\nabstract={Oxidative (respiratory) burst is an important manifestation of inflammation. Precise quantitative assessment of this reaction by flow cytometry made it possible to record and evaluate the severity of the inflammatory processes in a wide spectrum of diseases including diphtheria, hepatitis, pneumonia, bronchial asthma, arthritis, vasculitis, postoperative complications, tuberculosis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and myocardial infarction. This approach can be employed as a highly sensitive method of detection of inflammatory reactions and monitoring of their course in various pathological processes. © 2010 Springer Science+Business Media, Inc.},\r\nauthor_keywords={flow cytometry;  inflammation;  respiratory burst},\r\ncorrespondence_address1={Varfolomeeva, E. Y.; St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation; email: e_varf@mail.ru},\r\nissn={00074888},\r\ncoden={BEXBA},\r\npubmed_id={21234449},\r\nlanguage={English},\r\nabbrev_source_title={Bull. Exp. Biol. Med.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Oxidative (respiratory) burst is an important manifestation of inflammation. Precise quantitative assessment of this reaction by flow cytometry made it possible to record and evaluate the severity of the inflammatory processes in a wide spectrum of diseases including diphtheria, hepatitis, pneumonia, bronchial asthma, arthritis, vasculitis, postoperative complications, tuberculosis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and myocardial infarction. This approach can be employed as a highly sensitive method of detection of inflammatory reactions and monitoring of their course in various pathological processes. © 2010 Springer Science+Business Media, Inc.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&amp;partnerID=40&amp;md5=8a292ba5a5e9c827cff8d9e690c24c38\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&amp;partnerID=40&amp;md5=8a292ba5a5e9c827cff8d9e690c24c38', event);\">\n    Immunological similarity of diphtheria toxin and EGF receptor.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Alexeyev, V.; Kaboev, O.; Scherbakova, O.; Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 52(5): 364-370.  2010.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&amp;partnerID=40&amp;md5=8a292ba5a5e9c827cff8d9e690c24c38\"\n     onclick=\"log_download('alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&amp;partnerID=40&amp;md5=8a292ba5a5e9c827cff8d9e690c24c38', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Immunological similarity of diphtheria toxin and EGF receptor [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('alexeyev-kaboev-scherbakova-semenova-filatov-immunologicalsimilarityofdiphtheriatoxinandegfreceptor-2010')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Alexeyev2010364,\r\nauthor={Alexeyev, V.Yu. and Kaboev, O.K. and Scherbakova, O.G. and Semenova, E.V. and Filatov, M.V.},\r\ntitle={Immunological similarity of diphtheria toxin and EGF receptor},\r\njournal={Tsitologiya},\r\nyear={2010},\r\nvolume={52},\r\nnumber={5},\r\npages={364-370},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956413511&amp;partnerID=40&amp;md5=8a292ba5a5e9c827cff8d9e690c24c38},\r\naffiliation={Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States; Molecular and Radiation Biophysics Division of St. Petersburg, Nuclear Physics Institute, RAS, St. Petersburg, Gatchina, Russian Federation; Department of Molecular and Cellular Physiology, Stanford University, Stanford, United States},\r\nabstract={Cardiomyopathy and neuropathy are the two commonly observed complications in diphtheria patients and in, some instances, individuals vaccinated against diphtheria. The nature of these complications remains not well understood. It was suggested that autoimmunity may play a role in the development of these afflictions. Based on functional similarities between diphtheria toxin (DT) and epidermal growth factor receptor (EGFR), which both can bind to the heparin-binding EGF-like growth factor (HB-EGF) precursors, we suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized by antibodies raised against EGFR and by serum antibodies from diphtheria patients. Moreover serum of diphtheria patients competitively inhibits binding of anti-EGFR antibodies to the receptor. The truncated diphtheria toxin without B-subunit could be detected by serum antibodies of diphtheria patients, but not by anti-EGFR antibodies. Collectively, these studies demonstrate cross-reactivity of antibodies raised against B-subunit of DT and extracellular domain of EGFR and suggest that clinically observed post-diphtheria complications may result from autoimmune inhibition of EGFR function and possible destruction of receptor-positive tissues.},\r\nauthor_keywords={Cross-immunoreactivity;  Diphtheria toxin;  Epidermal growth factor receptor;  Post-diphtheria complications},\r\ncorrespondence_address1={Alexeyev, V. Yu.; Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Jefferson Vaccine Center, Philadelphia, United States},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={20586270},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Cardiomyopathy and neuropathy are the two commonly observed complications in diphtheria patients and in, some instances, individuals vaccinated against diphtheria. The nature of these complications remains not well understood. It was suggested that autoimmunity may play a role in the development of these afflictions. Based on functional similarities between diphtheria toxin (DT) and epidermal growth factor receptor (EGFR), which both can bind to the heparin-binding EGF-like growth factor (HB-EGF) precursors, we suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized by antibodies raised against EGFR and by serum antibodies from diphtheria patients. Moreover serum of diphtheria patients competitively inhibits binding of anti-EGFR antibodies to the receptor. The truncated diphtheria toxin without B-subunit could be detected by serum antibodies of diphtheria patients, but not by anti-EGFR antibodies. Collectively, these studies demonstrate cross-reactivity of antibodies raised against B-subunit of DT and extracellular domain of EGFR and suggest that clinically observed post-diphtheria complications may result from autoimmune inhibition of EGFR function and possible destruction of receptor-positive tissues.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&amp;doi=10.1134%2fS1063783410050379&amp;partnerID=40&amp;md5=b7266c4aa86093bcd4dc258952790714\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&amp;doi=10.1134%2fS1063783410050379&amp;partnerID=40&amp;md5=b7266c4aa86093bcd4dc258952790714', event);\">\n    Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Isaev-Ivanov, V.; Lebedev, D.; Lauter, H.; Pantina, R.; Kuklin, A.; Islamov, A.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Physics of the Solid State</i>, 52(5): 1063-1073.  2010.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&amp;doi=10.1134%2fS1063783410050379&amp;partnerID=40&amp;md5=b7266c4aa86093bcd4dc258952790714\"\n     onclick=\"log_download('isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&amp;doi=10.1134%2fS1063783410050379&amp;partnerID=40&amp;md5=b7266c4aa86093bcd4dc258952790714', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1063783410050379\"\n     onclick=\"log_download('isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010', 'http://doi.org/10.1134/S1063783410050379', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('isaevivanov-lebedev-lauter-pantina-kuklin-islamov-filatov-comparativeanalysisofthenucleosomestructureofcellnucleibysmallangleneutronscattering-2010')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Isaev-Ivanov20101063,\r\nauthor={Isaev-Ivanov, V.V. and Lebedev, D.V. and Lauter, H. and Pantina, R.A. and Kuklin, A.I. and Islamov, A.K. and Filatov, M.V.},\r\ntitle={Comparative analysis of the nucleosome structure of cell nuclei by small-angle neutron scattering},\r\njournal={Physics of the Solid State},\r\nyear={2010},\r\nvolume={52},\r\nnumber={5},\r\npages={1063-1073},\r\ndoi={10.1134/S1063783410050379},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952334920&amp;doi=10.1134%2fS1063783410050379&amp;partnerID=40&amp;md5=b7266c4aa86093bcd4dc258952790714},\r\naffiliation={Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; Research and Educational Center Biophysics of the St. Petersburg State Polytechnical University, Konstantinov Petersburg Nuclear Physics Institute of the Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Institut Laue-Langevin, BP 156, Grenoble 38042, France; Joint Institute for Nuclear Research, ul. Joliot-Curie 6, Dubna, Moscow oblast 141980, Russian Federation},\r\nabstract={The nucleosome structure in native nuclei of normal (chicken erythrocyte and rat leukocyte nuclei) and anomalously proliferating (the human cervical adenocarcinoma cell line HeLa and the Chinese hamster fibroblast cell line A238) cells has been investigated using small-angle neutron scattering. The experimental results obtained allow one to make the inference that the parameters of the nucleosome structure for the chicken erythrocyte and rat leukocyte nuclei (on average over the nucleus) are close to the universally accepted values and that the distance distribution function is bimodal. The bimodality of the distance distribution function reflects a narrow distribution of distances between nucleosomes (on average over the nucleus) at the fibril level of the chromatin organization. The histone core of the nucleosome structure in the nuclei of the HeLa and A238 cells (on average over the nucleus) is considerably less compact than that in the chicken erythrocyte and rat leukocyte nuclei, and the distance distribution function does not exhibit indications of the bimodality. © 2010 Pleiades Publishing, Ltd.},\r\ncorrespondence_address1={Filatov, M. V.; Konstantinov Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningradskaya oblast 188300, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\r\nissn={10637834},\r\nlanguage={English},\r\nabbrev_source_title={Phys. Solid State},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The nucleosome structure in native nuclei of normal (chicken erythrocyte and rat leukocyte nuclei) and anomalously proliferating (the human cervical adenocarcinoma cell line HeLa and the Chinese hamster fibroblast cell line A238) cells has been investigated using small-angle neutron scattering. The experimental results obtained allow one to make the inference that the parameters of the nucleosome structure for the chicken erythrocyte and rat leukocyte nuclei (on average over the nucleus) are close to the universally accepted values and that the distance distribution function is bimodal. The bimodality of the distance distribution function reflects a narrow distribution of distances between nucleosomes (on average over the nucleus) at the fibril level of the chromatin organization. The histone core of the nucleosome structure in the nuclei of the HeLa and A238 cells (on average over the nucleus) is considerably less compact than that in the chicken erythrocyte and rat leukocyte nuclei, and the distance distribution function does not exhibit indications of the bimodality. © 2010 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&amp;doi=10.1111%2fj.1600-0854.2010.01033.x&amp;partnerID=40&amp;md5=58b7e08a45bab6703d592151df3ebfcd\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&amp;doi=10.1111%2fj.1600-0854.2010.01033.x&amp;partnerID=40&amp;md5=58b7e08a45bab6703d592151df3ebfcd', event);\">\n    Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Angelotti, T.; Daunt, D.; Shcherbakova, O.; Kobilka, B.;  and Hurt, C.\n</span>\n\n  \n\n</span>\n\n  <i>Traffic</i>, 11(4): 560-578.  2010.\n  <span class=\"note\">cited By 29</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&amp;doi=10.1111%2fj.1600-0854.2010.01033.x&amp;partnerID=40&amp;md5=58b7e08a45bab6703d592151df3ebfcd\"\n     onclick=\"log_download('angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&amp;doi=10.1111%2fj.1600-0854.2010.01033.x&amp;partnerID=40&amp;md5=58b7e08a45bab6703d592151df3ebfcd', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1111/j.1600-0854.2010.01033.x\"\n     onclick=\"log_download('angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010', 'http://doi.org/10.1111/j.1600-0854.2010.01033.x', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('angelotti-daunt-shcherbakova-kobilka-hurt-regulationofgproteincoupledreceptortrafficbyanevolutionaryconservedhydrophobicsignal-2010')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Angelotti2010560,\r\nauthor={Angelotti, T. and Daunt, D. and Shcherbakova, O.G. and Kobilka, B. and Hurt, C.M.},\r\ntitle={Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal},\r\njournal={Traffic},\r\nyear={2010},\r\nvolume={11},\r\nnumber={4},\r\npages={560-578},\r\ndoi={10.1111/j.1600-0854.2010.01033.x},\r\nnote={cited By 29},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951731676&amp;doi=10.1111%2fj.1600-0854.2010.01033.x&amp;partnerID=40&amp;md5=58b7e08a45bab6703d592151df3ebfcd},\r\naffiliation={Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 259, Campus Drive, Stanford, CA 94035, United States},\r\nabstract={Plasma membrane (PM) expression of G-protein coupled receptors (GPCRs) is required for activation by extracellular ligands; however, mechanisms that regulate PM expression of GPCRs are poorly understood. For some GPCRs, such as alpha2c-adrenergic receptors (α2c-ARs), heterologous expression in non-native cells results in limited PM expression and extensive endoplasmic reticulum (ER) retention. Recently, ER export/retentions signals have been proposed to regulate cellular trafficking of several GPCRs. By utilizing a chimeric α2a/α2c-AR strategy, we identified an evolutionary conserved hydrophobic sequence (ALAAALAAAAA) in the extracellular amino terminal region that is responsible in part for α2c-AR subtype-specific trafficking. To our knowledge, this is the first luminal ER retention signal reported for a GPCR. Removal or disruption of the ER retention signal dramatically increased PM expression and decreased ER retention. Conversely, transplantation of this hydrophobic sequence into α2a-ARs reduced their PM expression and increased ER retention. This evolutionary conserved hydrophobic trafficking signal within α2c-ARs serves as a regulator of GPCR trafficking. © 2010 John Wiley &amp;amp; Sons A/S.},\r\nauthor_keywords={ER export;  ER retention;  GPCR trafficking;  Protein sorting;  Retention signal},\r\ncorrespondence_address1={Angelotti, T.; Department of Anesthesia, Stanford University School of Medicine, 300 Pasteur Drive, Grant Building S286, Stanford, CA 94305, United States; email: timangel@stanford.edu},\r\nissn={13989219},\r\ncoden={TRAFF},\r\npubmed_id={20059747},\r\nlanguage={English},\r\nabbrev_source_title={Traffic},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Plasma membrane (PM) expression of G-protein coupled receptors (GPCRs) is required for activation by extracellular ligands; however, mechanisms that regulate PM expression of GPCRs are poorly understood. For some GPCRs, such as alpha2c-adrenergic receptors (α2c-ARs), heterologous expression in non-native cells results in limited PM expression and extensive endoplasmic reticulum (ER) retention. Recently, ER export/retentions signals have been proposed to regulate cellular trafficking of several GPCRs. By utilizing a chimeric α2a/α2c-AR strategy, we identified an evolutionary conserved hydrophobic sequence (ALAAALAAAAA) in the extracellular amino terminal region that is responsible in part for α2c-AR subtype-specific trafficking. To our knowledge, this is the first luminal ER retention signal reported for a GPCR. Removal or disruption of the ER retention signal dramatically increased PM expression and decreased ER retention. Conversely, transplantation of this hydrophobic sequence into α2a-ARs reduced their PM expression and increased ER retention. This evolutionary conserved hydrophobic trafficking signal within α2c-ARs serves as a regulator of GPCR trafficking. © 2010 John Wiley &amp; Sons A/S.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2009\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2009')\"\n      onkeypress=\"toggleGroup('group_2009')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2009</span>\n      <span class=\"bibbase_group_count\">\n        \n        (4)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&amp;partnerID=40&amp;md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&amp;partnerID=40&amp;md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9', event);\">\n    Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Ėksperimental'naia i klinicheskaia gastroėnterologiia = Experimental & clinical gastroenterology</i>, (6): 138-142.  2009.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&amp;partnerID=40&amp;md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9\"\n     onclick=\"log_download('semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&amp;partnerID=40&amp;md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-possibilitiesofpharmacologicalhepatopathyandcorrectionoffatmetabolismviolationsindiabetesmellitustype1-2009')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2009138,\r\nauthor={Semenova, E.V.},\r\ntitle={Possibilities of pharmacological hepatopathy and correction of fat metabolism violations in diabetes mellitus type 1},\r\njournal={Ėksperimental&#x27;naia i klinicheskaia gastroėnterologiia = Experimental &amp; clinical gastroenterology},\r\nyear={2009},\r\nnumber={6},\r\npages={138-142},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952562838&amp;partnerID=40&amp;md5=a6d7a2a93c22bdc9c7fa9cefe9f249f9},\r\nabstract={The purpose of the research has been the study of possibilities for the pharmacological correction of the morphological changes in liver and lipid metabolism disorders in experimental type 1 diabetes. Simvastatin, new compound from the group of antioxidants--3-hydroxipyridine derivate under the code HP-5, and also the combination of simvastatin and HP-5 have been used for the pharmacological correction in this research. It was revealed that the using of HP-5 and the combination of simvastatin and HP-5 attenuate the order of expression of diabetic hepatopathia as the result of the histological study and hinder from the development of dislipidemia in rates with type 1 diabetes which is more expressed in comparison with the monotherapy by simvastatin. These changes also are followed with the diminishing of the free radical lipid oxidation activity by the specific antioxidant effect of the investigated 3-hydroxipyridine derivate.},\r\ncorrespondence_address1={Semenova, E.V.},\r\nissn={16828658},\r\npubmed_id={20201297},\r\nlanguage={Russian},\r\nabbrev_source_title={Eksp Klin Gastroenterol},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The purpose of the research has been the study of possibilities for the pharmacological correction of the morphological changes in liver and lipid metabolism disorders in experimental type 1 diabetes. Simvastatin, new compound from the group of antioxidants–3-hydroxipyridine derivate under the code HP-5, and also the combination of simvastatin and HP-5 have been used for the pharmacological correction in this research. It was revealed that the using of HP-5 and the combination of simvastatin and HP-5 attenuate the order of expression of diabetic hepatopathia as the result of the histological study and hinder from the development of dislipidemia in rates with type 1 diabetes which is more expressed in comparison with the monotherapy by simvastatin. These changes also are followed with the diminishing of the free radical lipid oxidation activity by the specific antioxidant effect of the investigated 3-hydroxipyridine derivate.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&amp;doi=10.1117%2f12.836452&amp;partnerID=40&amp;md5=f3819171ad8b578019e72414bc891193\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&amp;doi=10.1117%2f12.836452&amp;partnerID=40&amp;md5=f3819171ad8b578019e72414bc891193', event);\">\n    Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Galich, N.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n   2009.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 2; Conference of 12th International Workshop on Nanodesign Technology and Computer Simulations ; Conference Date: 23 June 2008 Through 27 June 2008; Conference Code:77311</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&amp;doi=10.1117%2f12.836452&amp;partnerID=40&amp;md5=f3819171ad8b578019e72414bc891193\"\n     onclick=\"log_download('galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&amp;doi=10.1117%2f12.836452&amp;partnerID=40&amp;md5=f3819171ad8b578019e72414bc891193', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1117/12.836452\"\n     onclick=\"log_download('galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009', 'http://doi.org/10.1117/12.836452', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('galich-filatov-delaychangeandbifurcationoftheimmunofluorescencedistributionattractorsinhealthstatusesdiagnosticsandinmedicaltreatment-2009')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Galich2009,\r\nauthor={Galich, N.E. and Filatov, M.V.},\r\ntitle={Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment},\r\njournal={Proceedings of SPIE - The International Society for Optical Engineering},\r\nyear={2009},\r\nvolume={7377},\r\ndoi={10.1117/12.836452},\r\nart_number={73770C},\r\nnote={cited By 2; Conference of 12th International Workshop on Nanodesign Technology and Computer Simulations ; Conference Date: 23 June 2008 Through 27 June 2008;  Conference Code:77311},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349977669&amp;doi=10.1117%2f12.836452&amp;partnerID=40&amp;md5=f3819171ad8b578019e72414bc891193},\r\naffiliation={Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; Department of Cell Biology, B.P.Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 183300, Russian Federation},\r\nabstract={Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations&#x27; in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations&#x27; fluorescence presents the sensitive clear indicator of health status. © 2009 SPIE.},\r\nauthor_keywords={Attractor;  Bifurcation;  Blood;  Cell;  Cytometry;  Distribution;  DNA;  Immunofluorescence;  Neutrophils;  Oxidants},\r\ncorrespondence_address1={Galich, N. E.; Department of Experimental Physics, St. Petersburg State Polytechnical University, Politechnicheskaya 29, St. Petersburg, 195251, Russian Federation; email: n.galich@mail.ru},\r\nsponsors={Motorola Company; Research and Production Corporation Integral; Scanwest Company; Silvaco Data Systems},\r\naddress={Minsk},\r\nissn={0277786X},\r\nisbn={9780819476531},\r\ncoden={PSISD},\r\nlanguage={English},\r\nabbrev_source_title={Proc SPIE Int Soc Opt Eng},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status. © 2009 SPIE.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&amp;partnerID=40&amp;md5=f39cf36c04fe528f017aa5d1c25c72f9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&amp;partnerID=40&amp;md5=f39cf36c04fe528f017aa5d1c25c72f9', event);\">\n    Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Shadrina, M.; Slominskiǐ, P.; Illarioshkin, S.; Bagyeva, G.; Karabanov, A.; Ivanova-Smolenskaia, I.;  and Limborskaia, S.\n</span>\n\n  \n\n</span>\n\n  <i>Genetika</i>, 45(4): 573-576.  2009.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&amp;partnerID=40&amp;md5=f39cf36c04fe528f017aa5d1c25c72f9\"\n     onclick=\"log_download('semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&amp;partnerID=40&amp;md5=f39cf36c04fe528f017aa5d1c25c72f9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-shadrina-slominski-illarioshkin-bagyeva-karabanov-ivanovasmolenskaia-limborskaia-analysisofthealphasynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsontsdisease-2009')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2009573,\r\nauthor={Semenova, E.V. and Shadrina, M.I. and Slominskiǐ, P.A. and Illarioshkin, S.N. and Bagyeva, G.K. and Karabanov, A.V. and Ivanova-Smolenskaia, I.A. and Limborskaia, S.A.},\r\ntitle={Analysis of the alpha-synuclein gene dosage variation associated with autosomal dominant form of ParkinsonTs disease},\r\njournal={Genetika},\r\nyear={2009},\r\nvolume={45},\r\nnumber={4},\r\npages={573-576},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649401972&amp;partnerID=40&amp;md5=f39cf36c04fe528f017aa5d1c25c72f9},\r\nabstract={Fifty-two patients that had ParkinsonTs disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4--6 of alpha-synuclein gene using real-time PCR with Taq-Man probes. No mutations involving the examined exons dosage were revealed in alpha-synuclein gene. Thus, mutations modifying copy number of alpha-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of ParkinsonTs disease in patients from Russia.},\r\ncorrespondence_address1={Semenova, E.V.},\r\nissn={00166758},\r\npubmed_id={19507712},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Fifty-two patients that had ParkinsonTs disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4–6 of alpha-synuclein gene using real-time PCR with Taq-Man probes. No mutations involving the examined exons dosage were revealed in alpha-synuclein gene. Thus, mutations modifying copy number of alpha-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of ParkinsonTs disease in patients from Russia.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&amp;doi=10.1134%2fS1022795409040176&amp;partnerID=40&amp;md5=317f7a9d80285dad4ca92bc42d619bdb\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&amp;doi=10.1134%2fS1022795409040176&amp;partnerID=40&amp;md5=317f7a9d80285dad4ca92bc42d619bdb', event);\">\n    Analysis of the α-synuclein gene dosage variation associated with autosomal dominant form of Parkinson's disease.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Genetics</i>, 45(4): 504-506.  2009.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&amp;doi=10.1134%2fS1022795409040176&amp;partnerID=40&amp;md5=317f7a9d80285dad4ca92bc42d619bdb\"\n     onclick=\"log_download('anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-65349183457&amp;doi=10.1134%2fS1022795409040176&amp;partnerID=40&amp;md5=317f7a9d80285dad4ca92bc42d619bdb', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of the α-synuclein gene dosage variation associated with autosomal dominant form of Parkinson&#x27;s disease [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1022795409040176\"\n     onclick=\"log_download('anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009', 'http://doi.org/10.1134/S1022795409040176', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-analysisofthesynucleingenedosagevariationassociatedwithautosomaldominantformofparkinsonsdisease-2009')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Fifty-two patients that had Parkinson's disease with autosomal dominant type of inheritance were analyzed for the presence of duplications and triplications in exons 4-6 of α-synuclein gene using real-time PCR with TaqMan probes. No mutations involving the examined exons dosage were revealed in α-synuclein gene. Thus, mutations modifying copy number of α-synuclein gene do not significantly affect the pathogenesis of the autosomal dominant form of Parkinson's disease in patients from Russia. © 2009 Pleiades Publishing, Ltd.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2008\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2008')\"\n      onkeypress=\"toggleGroup('group_2008')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2008</span>\n      <span class=\"bibbase_group_count\">\n        \n        (4)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&amp;partnerID=40&amp;md5=5313418d75141a24ef0926ff2a58d2ff\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&amp;partnerID=40&amp;md5=5313418d75141a24ef0926ff2a58d2ff', event);\">\n    Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Varfolomeeva, E.; Semenova, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 50(11): 958-963.  2008.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&amp;partnerID=40&amp;md5=5313418d75141a24ef0926ff2a58d2ff\"\n     onclick=\"log_download('shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&amp;partnerID=40&amp;md5=5313418d75141a24ef0926ff2a58d2ff', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-varfolomeeva-semenova-filatov-humanrad51recombinasetheroleinthecellcyclecheckpointandcellularsurvival-2008')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2008958,\r\nauthor={Shtam, T.A. and Varfolomeeva, E.Y. and Semenova, E.V. and Filatov, M.V.},\r\ntitle={Human rad51 recombinase: The role in the cell cycle checkpoint and cellular survival},\r\njournal={Tsitologiya},\r\nyear={2008},\r\nvolume={50},\r\nnumber={11},\r\npages={958-963},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-59449089348&amp;partnerID=40&amp;md5=5313418d75141a24ef0926ff2a58d2ff},\r\naffiliation={Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},\r\nabstract={The RAD 51 protein, an eukaryotic homologue of Escherichia coli Rec A, plays a central role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of Rad5l gene by specific small interfering (si) RNA induces cell death of the most part of investigated cancer cell lines and normal fibroblasts. Disruption of the Rad51 gene in these cells results in S or(and) G 2 cell cycle arrest leading to apoptosis. But some human cancer cell lines demonstrate abolishment of premitotic checkpoint and are not sensitive to siRNA silencing of RAD51 recombinase. Recent experiments show that normal functioning of the recombination repair system is essential for maintenance of genome stability, proliferation of vertebrate cells and, finally, for prevention of dramatic cell death.},\r\nauthor_keywords={Cell cycle;  DNA reparation;  Homologous recombination;  Rad51},\r\ncorrespondence_address1={Shtam, T. A.; Petersburg B. P. Konstantinov Nuclear Physics Institute RAS, Gatchina, Russian Federation},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={19140342},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The RAD 51 protein, an eukaryotic homologue of Escherichia coli Rec A, plays a central role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of Rad5l gene by specific small interfering (si) RNA induces cell death of the most part of investigated cancer cell lines and normal fibroblasts. Disruption of the Rad51 gene in these cells results in S or(and) G 2 cell cycle arrest leading to apoptosis. But some human cancer cell lines demonstrate abolishment of premitotic checkpoint and are not sensitive to siRNA silencing of RAD51 recombinase. Recent experiments show that normal functioning of the recombination repair system is essential for maintenance of genome stability, proliferation of vertebrate cells and, finally, for prevention of dramatic cell death.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&amp;doi=10.1134%2fS1990519X08050027&amp;partnerID=40&amp;md5=30d4ecc52e19dc81c11a7ab184a77380\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&amp;doi=10.1134%2fS1990519X08050027&amp;partnerID=40&amp;md5=30d4ecc52e19dc81c11a7ab184a77380', event);\">\n    Role of human RAD51 recombinase in the cycle checkpoint and survival of a cell.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Cell and Tissue Biology</i>, 2(5): 463-467.  2008.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&amp;doi=10.1134%2fS1990519X08050027&amp;partnerID=40&amp;md5=30d4ecc52e19dc81c11a7ab184a77380\"\n     onclick=\"log_download('anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-54949089066&amp;doi=10.1134%2fS1990519X08050027&amp;partnerID=40&amp;md5=30d4ecc52e19dc81c11a7ab184a77380', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Role of human RAD51 recombinase in the cycle checkpoint and survival of a cell [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1990519X08050027\"\n     onclick=\"log_download('anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008', 'http://doi.org/10.1134/S1990519X08050027', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-roleofhumanrad51recombinaseinthecyclecheckpointandsurvivalofacell-2008')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The RAD 51 protein, a eukaryotic homologue of Escherichia coli RecA, plays a significant role in both mitotic and meiotic homologous recombination. Here, we demonstrate that short-term silencing of the Rad51 gene by specific small interfering RNA (siRNA) that inhibits cell proliferation and reduces the viability of most cells. Cells with suppressed expression of Rad51 gene have altered cell cycles and accumulate in the S and G2 phases. Our findings show that the disruption of homologous recombination leads to cell death. However, some cells, e.g., MCF-7 cells, are insensitive to the suppression of Rad51 gene expression. © MAIK Nauka 2008.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&amp;partnerID=40&amp;md5=a30b0b5ac1d900684a623822c2fcc6b0\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&amp;partnerID=40&amp;md5=a30b0b5ac1d900684a623822c2fcc6b0', event);\">\n    Study of plasma megamolecular complexation by laser correlation spectroscopy.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Landa, S.; Filatov, M.; Arutyunyan, A.;  and Varfolomeyeva, Y.\n</span>\n\n  \n\n</span>\n\n  <i>Klinichescheskaya Laboratornaya Diagnostika</i>, (4): 37-41.  2008.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&amp;partnerID=40&amp;md5=a30b0b5ac1d900684a623822c2fcc6b0\"\n     onclick=\"log_download('landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&amp;partnerID=40&amp;md5=a30b0b5ac1d900684a623822c2fcc6b0', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Study of plasma megamolecular complexation by laser correlation spectroscopy [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('landa-filatov-arutyunyan-varfolomeyeva-studyofplasmamegamolecularcomplexationbylasercorrelationspectroscopy-2008')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Landa200837,\r\nauthor={Landa, S.B. and Filatov, M.V. and Arutyunyan, A.V. and Varfolomeyeva, Ye.V.},\r\ntitle={Study of plasma megamolecular complexation by laser correlation spectroscopy},\r\njournal={Klinichescheskaya Laboratornaya Diagnostika},\r\nyear={2008},\r\nnumber={4},\r\npages={37-41},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46749109313&amp;partnerID=40&amp;md5=a30b0b5ac1d900684a623822c2fcc6b0},\r\nissn={08692084},\r\npubmed_id={18619204},\r\nlanguage={Russian},\r\nabbrev_source_title={Klin. Lab. Diagn.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&amp;doi=10.1134%2fS1063774508010136&amp;partnerID=40&amp;md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&amp;doi=10.1134%2fS1063774508010136&amp;partnerID=40&amp;md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf', event);\">\n    Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Lebedev, D.; Filatov, M.; Kuklin, A.; Islamov, A.; Stellbrink, J.; Pantina, R.; Denisov, Y.; Toperverg, B.;  and Isaev-Ivanov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Crystallography Reports</i>, 53(1): 110-115.  2008.\n  <span class=\"note\">cited By 18</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&amp;doi=10.1134%2fS1063774508010136&amp;partnerID=40&amp;md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf\"\n     onclick=\"log_download('lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&amp;doi=10.1134%2fS1063774508010136&amp;partnerID=40&amp;md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1063774508010136\"\n     onclick=\"log_download('lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008', 'http://doi.org/10.1134/S1063774508010136', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('lebedev-filatov-kuklin-islamov-stellbrink-pantina-denisov-toperverg-etal-structuralhierarchyofchromatininchickenerythrocytenucleibasedonsmallangleneutronscatteringfractalnatureofthelargescalechromatinorganization-2008')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Lebedev2008110,\r\nauthor={Lebedev, D.V. and Filatov, M.V. and Kuklin, A.I. and Islamov, A.Kh. and Stellbrink, J. and Pantina, R.A. and Denisov, Yu.Yu. and Toperverg, B.P. and Isaev-Ivanov, V.V.},\r\ntitle={Structural hierarchy of chromatin in chicken erythrocyte nuclei based on small-angle neutron scattering: Fractal nature of the large-scale chromatin organization},\r\njournal={Crystallography Reports},\r\nyear={2008},\r\nvolume={53},\r\nnumber={1},\r\npages={110-115},\r\ndoi={10.1134/S1063774508010136},\r\nnote={cited By 18},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-38749093755&amp;doi=10.1134%2fS1063774508010136&amp;partnerID=40&amp;md5=cfa0e3cb31cc3b6a1cf7674af5cbbbbf},\r\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, ul. Orlova Roshcha 1, Gatchina, Leningrad oblast 188300, Russian Federation; Biofizika Research and Education Center, St. Petersburg State Polytechnic University, Russian Academy of Sciences, St. Petersburg 195251, Russian Federation; Joint Institute of Nuclear Research, ul. Zholio-Kyuri 6, Dubna, Moscow Oblast 141980, Russian Federation; Research Centre Jülich, Jülich 52425, Germany},\r\nabstract={The chromatin organization in chicken erythrocyte nuclei was studied by small-angle neutron scattering in the scattering-vector range from 1.5 × 10-1 to 10-4 Å-1 with the use of the contrast-variation technique. This scattering-vector range corresponds to linear dimensions from 4 nm to 6 μm and covers the whole hierarchy of chromatin structures, from the nucleosomal structure to the entire nucleus. The results of the present study allowed the following conclusions to be drawn: (1) both the chromatin-protein structure and the structure of the nucleic acid component in chicken erythrocyte nuclei have mass-fractal properties, (2) the structure of the protein component of chromatin exhibits a fractal behavior on scales extending over two orders of magnitude, from the nucleosomal size to the size of an entire nucleus, and (3) the structure of the nucleic acid component of chromatin in chicken erythrocyte nuclei is likewise of a fractal nature and has two levels of organization or two phases with the crossover point at about 300-400 nm. © 2008 Pleiades Publishing, Inc.},\r\nfunding_details={Российский Фонд Фундаментальных Исследований (РФФИ)02-04-49259-a},\r\nfunding_details={Ministry of Education and Science of the Russian FederationRNP 2.2.1.1.4663},\r\nfunding_details={Ministry of Education and Science of the Russian Federation40.012.1.1.1149},\r\n}</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The chromatin organization in chicken erythrocyte nuclei was studied by small-angle neutron scattering in the scattering-vector range from 1.5 × 10-1 to 10-4 Å-1 with the use of the contrast-variation technique. This scattering-vector range corresponds to linear dimensions from 4 nm to 6 μm and covers the whole hierarchy of chromatin structures, from the nucleosomal structure to the entire nucleus. The results of the present study allowed the following conclusions to be drawn: (1) both the chromatin-protein structure and the structure of the nucleic acid component in chicken erythrocyte nuclei have mass-fractal properties, (2) the structure of the protein component of chromatin exhibits a fractal behavior on scales extending over two orders of magnitude, from the nucleosomal size to the size of an entire nucleus, and (3) the structure of the nucleic acid component of chromatin in chicken erythrocyte nuclei is likewise of a fractal nature and has two levels of organization or two phases with the crossover point at about 300-400 nm. © 2008 Pleiades Publishing, Inc.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2007\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2007')\"\n      onkeypress=\"toggleGroup('group_2007')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2007</span>\n      <span class=\"bibbase_group_count\">\n        \n        (7)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&amp;doi=10.1002%2f9783527613489.ch59&amp;partnerID=40&amp;md5=41038eec12b571fb984fee210e4729d5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&amp;doi=10.1002%2f9783527613489.ch59&amp;partnerID=40&amp;md5=41038eec12b571fb984fee210e4729d5', event);\">\n    Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Naryzhny, S.; Levina, V.; Varfolomeeva, E.; Drobchenko, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  Wiley Blackwell,  2007.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&amp;doi=10.1002%2f9783527613489.ch59&amp;partnerID=40&amp;md5=41038eec12b571fb984fee210e4729d5\"\n     onclick=\"log_download('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&amp;doi=10.1002%2f9783527613489.ch59&amp;partnerID=40&amp;md5=41038eec12b571fb984fee210e4729d5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/9783527613489.ch59\"\n     onclick=\"log_download('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007', 'http://doi.org/10.1002/9783527613489.ch59', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@BOOK{Naryzhny2007453,\r\nauthor={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},\r\ntitle={Active dissociation of the fluorescent dye hoechst 33342 from DNA in a living cell: Who could do it},\r\njournal={From Genome to Proteome: Advances in the Practice &amp; Application of Proteomics},\r\nyear={2007},\r\npages={453-458},\r\ndoi={10.1002/9783527613489.ch59},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956551553&amp;doi=10.1002%2f9783527613489.ch59&amp;partnerID=40&amp;md5=41038eec12b571fb984fee210e4729d5},\r\naffiliation={Petersburg Nuclear Physics Institute of Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation},\r\nabstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA. © 2000 WILEY-VCH Verlag GmbH. All rights reserved.},\r\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional Polyacrylamide gel electrophoresis},\r\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute of Russian Academy of SciencesRussian Federation; email: naryzhny@omrb.pnpi.spb.ru},\r\npublisher={Wiley Blackwell},\r\nisbn={9783527613489; 9783527301546},\r\nlanguage={English},\r\nabbrev_source_title={From Genome to Proteome: Adv. in the Pract. &amp; Appl. of Proteomics},\r\ndocument_type={Book Chapter},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA. © 2000 WILEY-VCH Verlag GmbH. All rights reserved.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&amp;partnerID=40&amp;md5=dd7854f4ddd307cb94f5c9ebb3362452\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&amp;partnerID=40&amp;md5=dd7854f4ddd307cb94f5c9ebb3362452', event);\">\n    Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shtam, T.; Pantina, R.; Varfolomeyeva, Y.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Voprosy Onkologii</i>, 53(2): 194-199.  2007.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&amp;partnerID=40&amp;md5=dd7854f4ddd307cb94f5c9ebb3362452\"\n     onclick=\"log_download('shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&amp;partnerID=40&amp;md5=dd7854f4ddd307cb94f5c9ebb3362452', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shtam-pantina-varfolomeyeva-filatov-responsetotamoxifeninestrogenreceptorpositivecelllinemcf7isindependentofp53expression-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shtam2007194,\r\nauthor={Shtam, T.A. and Pantina, R.A. and Varfolomeyeva, Ye.Yu. and Filatov, M.V.},\r\ntitle={Response to tamoxifen in estrogen receptor-positive cell line MCF-7 is independent of p53 expression},\r\njournal={Voprosy Onkologii},\r\nyear={2007},\r\nvolume={53},\r\nnumber={2},\r\npages={194-199},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547598534&amp;partnerID=40&amp;md5=dd7854f4ddd307cb94f5c9ebb3362452},\r\naffiliation={B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},\r\nabstract={The study was concerned with identification of predictive value of p53 expression on sensitivity to tamoxifen in breast cancer management. Estrogen receptor-positive cell line MCF-7 was used to establish p53 expression influence on the rate of cell proliferation after tamoxifen. The investigation demonstrated the absence of that effect when p53 was silenced.},\r\ncorrespondence_address1={Shtam, T. A.; B.P.Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St.Petersburg, Russian Federation},\r\nissn={05073758},\r\ncoden={VOONA},\r\nlanguage={Russian},\r\nabbrev_source_title={Vopr. Onkol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The study was concerned with identification of predictive value of p53 expression on sensitivity to tamoxifen in breast cancer management. Estrogen receptor-positive cell line MCF-7 was used to establish p53 expression influence on the rate of cell proliferation after tamoxifen. The investigation demonstrated the absence of that effect when p53 was silenced.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&amp;partnerID=40&amp;md5=e4f22788b05bbb2755dfab001ca19b6e\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&amp;partnerID=40&amp;md5=e4f22788b05bbb2755dfab001ca19b6e', event);\">\n    Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.;  and Inchina, V.\n</span>\n\n  \n\n</span>\n\n  <i>Ėksperimental'nai&x0361a i klinicheskai&x0361a gastroėnterologii&x0361a = Experimental & clinical gastroenterology</i>, (6): 122-125, 131.  2007.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&amp;partnerID=40&amp;md5=e4f22788b05bbb2755dfab001ca19b6e\"\n     onclick=\"log_download('semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&amp;partnerID=40&amp;md5=e4f22788b05bbb2755dfab001ca19b6e', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-inchina-studyofhepatoprotectiveactivityofethylmethylhydroxypyridinefumarateandlipoateintheexperimentaltoxichepatitis-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2007,\r\nauthor={Semenova, E.V. and Inchina, V.I.},\r\ntitle={Study of hepatoprotective activity of ethylmethylhydroxypyridine fumarate and lipoate in the experimental toxic hepatitis},\r\njournal={Ėksperimental&#x27;nai&amp;x0361a i klinicheskai&amp;x0361a gastroėnterologii&amp;x0361a = Experimental &amp; clinical gastroenterology},\r\nyear={2007},\r\nnumber={6},\r\npages={122-125, 131},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-46649109183&amp;partnerID=40&amp;md5=e4f22788b05bbb2755dfab001ca19b6e},\r\nabstract={The authors have studied hepatoprotective actions of new derivatives of 3-hydroxipyridine on an experimental model of toxic hepatitis (140 white mice). Mexidol and berlithion are choosed as the preparations of comparison. The method of light microscopy is used for the exploration of morphological changes. The cytolytic contents activity, catalase activity and the level of MDA have been determined in blood serum. The antitoxic effect is valued by the survival of the animals. It is found that all examined bonds is corrected morphological changes in toxic hepatitis and increased the index of animals survival, which is more expressed than the preparations of comparison.},\r\ncorrespondence_address1={Semenova, E.V.},\r\nissn={16828658},\r\npubmed_id={18416106},\r\nlanguage={Russian},\r\nabbrev_source_title={Eksp Klin Gastroenterol},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The authors have studied hepatoprotective actions of new derivatives of 3-hydroxipyridine on an experimental model of toxic hepatitis (140 white mice). Mexidol and berlithion are choosed as the preparations of comparison. The method of light microscopy is used for the exploration of morphological changes. The cytolytic contents activity, catalase activity and the level of MDA have been determined in blood serum. The antitoxic effect is valued by the survival of the animals. It is found that all examined bonds is corrected morphological changes in toxic hepatitis and increased the index of animals survival, which is more expressed than the preparations of comparison.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&amp;doi=10.1117%2f12.726756&amp;partnerID=40&amp;md5=1e708809433caac2e8f0ce3082166aba\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&amp;doi=10.1117%2f12.726756&amp;partnerID=40&amp;md5=1e708809433caac2e8f0ce3082166aba', event);\">\n    Laser fluorescence fluctuation excesses in molecular immunology experiments.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Galich, N.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n   2007.<!-- NOTE FROM BIBBASE: This entry has an invalid bibtex entry type: conference. Therefore, we don't know how to render it properly. Please consider fixing this. -->\n  <span class=\"note\">cited By 5; Conference of Nanodesign, Technology, and Computer Simulations ; Conference Date: 19 June 2006 Through 25 June 2006; Conference Code:70585</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&amp;doi=10.1117%2f12.726756&amp;partnerID=40&amp;md5=1e708809433caac2e8f0ce3082166aba\"\n     onclick=\"log_download('galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&amp;doi=10.1117%2f12.726756&amp;partnerID=40&amp;md5=1e708809433caac2e8f0ce3082166aba', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Laser fluorescence fluctuation excesses in molecular immunology experiments [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1117/12.726756\"\n     onclick=\"log_download('galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007', 'http://doi.org/10.1117/12.726756', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('galich-filatov-laserfluorescencefluctuationexcessesinmolecularimmunologyexperiments-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@CONFERENCE{Galich2007,\r\nauthor={Galich, N.E. and Filatov, M.V.},\r\ntitle={Laser fluorescence fluctuation excesses in molecular immunology experiments},\r\njournal={Proceedings of SPIE - The International Society for Optical Engineering},\r\nyear={2007},\r\nvolume={6597},\r\ndoi={10.1117/12.726756},\r\nart_number={65970L},\r\nnote={cited By 5; Conference of Nanodesign, Technology, and Computer Simulations ; Conference Date: 19 June 2006 Through 25 June 2006;  Conference Code:70585},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36249003315&amp;doi=10.1117%2f12.726756&amp;partnerID=40&amp;md5=1e708809433caac2e8f0ce3082166aba},\r\naffiliation={St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation; B.P.Konstantinov Petersburg Nuclear Physics Institute, St.Petersburg, 183300, Russian Federation},\r\nabstract={A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.},\r\nauthor_keywords={Cytofluorescence histogram;  Excesses;  Inflammation;  Respiratory burst;  Statistic analysis},\r\ncorrespondence_address1={Galich, N.E.; St. Petersburg Polytechnical University, Polytekhnicheskaya 29, St. Petersburg 195251, Russian Federation},\r\nsponsors={SPIE Poland Chapter; SPIE Russia Chapter},\r\naddress={Olsztyn},\r\nissn={0277786X},\r\nisbn={0819467332; 9780819467331},\r\ncoden={PSISD},\r\nlanguage={English},\r\nabbrev_source_title={Proc SPIE Int Soc Opt Eng},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A novel approach to statistical analysis of flow cytometry fluorescence data have been developed and applied for population analysis of blood neutrophils stained with hydroethidine during respiratory burst reaction. The staining based on intracellular oxidation hydroethidine to ethidium bromide, which intercalate into cell DNA. Fluorescence of the resultant product serves as a measure of the neutrophil ability to generate superoxide radicals after induction respiratory burst reaction by phorbol myristate acetate (PMA). It was demonstrated that polymorphonuclear leukocytes of persons with inflammatory diseases showed a considerably changed response. Cytofluorometric histograms obtained have unique information about condition of neutrophil population what might to allow a determination of the pathology processes type connecting with such inflammation. A novel approach to histogram analysis is based on analysis of high-momentum dynamic of distribution. The features of fluctuation excesses of distribution have unique information about disease under consideration.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&amp;doi=10.1038%2fsj.cr.2007.90&amp;partnerID=40&amp;md5=9a31d0fb63047d78fac19588b492e312\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&amp;doi=10.1038%2fsj.cr.2007.90&amp;partnerID=40&amp;md5=9a31d0fb63047d78fac19588b492e312', event);\">\n    IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Carey, G.; Semenova, E.; Qi, X.;  and Keegan, A.\n</span>\n\n  \n\n</span>\n\n  <i>Cell Research</i>, 17(11): 942-955.  2007.\n  <span class=\"note\">cited By 24</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&amp;doi=10.1038%2fsj.cr.2007.90&amp;partnerID=40&amp;md5=9a31d0fb63047d78fac19588b492e312\"\n     onclick=\"log_download('carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&amp;doi=10.1038%2fsj.cr.2007.90&amp;partnerID=40&amp;md5=9a31d0fb63047d78fac19588b492e312', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1038/sj.cr.2007.90\"\n     onclick=\"log_download('carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007', 'http://doi.org/10.1038/sj.cr.2007.90', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('carey-semenova-qi-keegan-il4protectsthebcelllymphomacelllinech31fromantiigminducedgrowtharrestandapoptosiscontributionofthepi3kinaseaktpathway-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Carey2007942,\r\nauthor={Carey, G.B. and Semenova, E. and Qi, X. and Keegan, A.D.},\r\ntitle={IL-4 protects the B-cell lymphoma cell line CH31 from anti-IgM-induced growth arrest and apoptosis: Contribution of the PI-3 kinase/AKT pathway},\r\njournal={Cell Research},\r\nyear={2007},\r\nvolume={17},\r\nnumber={11},\r\npages={942-955},\r\ndoi={10.1038/sj.cr.2007.90},\r\nnote={cited By 24},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36248965713&amp;doi=10.1038%2fsj.cr.2007.90&amp;partnerID=40&amp;md5=9a31d0fb63047d78fac19588b492e312},\r\naffiliation={Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland of School of Medicine, Baltimore, MD, United States},\r\nabstract={Interleukin-4 (IL-4) promotes lymphocyte survival and protects primary lymphomas from apoptosis. Previous studies reported differential requirements for the signal transducer and activator of transcription 6 (STAT6) and IRS2/phosphatidylinositol 3 kinase (PI-3K) signaling pathways in mediating the IL-4-induced protection from Fas-mediated apoptosis. In this study, we characterized IL-4-activated signals that suppress anti-IgM-mediated apoptosis and growth arrest of CH31, a model B-cell lymphoma line. In CH31, anti-IgM treatment leads to the loss of mitochondrial membrane potential, phospho-Akt, phospho-CDK2, and c-myc protein. These losses are followed by massive induction of p27Kip1 protein expression, cell cycle arrest, and apoptosis. Strikingly, IL-4 treatment prevented or reversed these changes. Furthermore, IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD. IL-4 treatment also induced expression of BclxL, a STAT6-dependent gene. Pharmacologic inhibitors and dominant inhibitory forms of PI-3K and Akt abrogated the anti-apoptotic function of IL-4. These results suggest that the IL-4 receptor activates several signaling pathways, with the Akt pathway playing a major role in suppression of the apoptotic program activated by anti-IgM. © 2007 IBCB, SIBS, CAS. All rights reserved.},\r\nauthor_keywords={Akt;  Apoptosis;  Cell cycle;  IL-4;  PI-3 kinase},\r\ncorrespondence_address1={Keegan, A.D.; Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland of School of Medicine, Baltimore, MD, United States; email: akeegan@som.umaryland.edu},\r\nissn={10010602},\r\npubmed_id={17968425},\r\nlanguage={English},\r\nabbrev_source_title={Cell Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Interleukin-4 (IL-4) promotes lymphocyte survival and protects primary lymphomas from apoptosis. Previous studies reported differential requirements for the signal transducer and activator of transcription 6 (STAT6) and IRS2/phosphatidylinositol 3 kinase (PI-3K) signaling pathways in mediating the IL-4-induced protection from Fas-mediated apoptosis. In this study, we characterized IL-4-activated signals that suppress anti-IgM-mediated apoptosis and growth arrest of CH31, a model B-cell lymphoma line. In CH31, anti-IgM treatment leads to the loss of mitochondrial membrane potential, phospho-Akt, phospho-CDK2, and c-myc protein. These losses are followed by massive induction of p27Kip1 protein expression, cell cycle arrest, and apoptosis. Strikingly, IL-4 treatment prevented or reversed these changes. Furthermore, IL-4 suppressed the activation of caspases 9 and 3, and, in contrast to previous reports, induced the phosphorylation (deactivation) of BAD. IL-4 treatment also induced expression of BclxL, a STAT6-dependent gene. Pharmacologic inhibitors and dominant inhibitory forms of PI-3K and Akt abrogated the anti-apoptotic function of IL-4. These results suggest that the IL-4 receptor activates several signaling pathways, with the Akt pathway playing a major role in suppression of the apoptotic program activated by anti-IgM. © 2007 IBCB, SIBS, CAS. All rights reserved.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&amp;doi=10.1186%2f1471-2350-8-6&amp;partnerID=40&amp;md5=bb4117dd790d75c9fea41d1ab154724f\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&amp;doi=10.1186%2f1471-2350-8-6&amp;partnerID=40&amp;md5=bb4117dd790d75c9fea41d1ab154724f', event);\">\n    Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shadrina, M.; Semenova, E.; Slominsky, P.; Bagyeva, G.; Illarioshkin, S.; Ivanova-Smolenskaia, I.;  and Limborska, S.\n</span>\n\n  \n\n</span>\n\n  <i>BMC Medical Genetics</i>, 8.  2007.\n  <span class=\"note\">cited By 33</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&amp;doi=10.1186%2f1471-2350-8-6&amp;partnerID=40&amp;md5=bb4117dd790d75c9fea41d1ab154724f\"\n     onclick=\"log_download('shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&amp;doi=10.1186%2f1471-2350-8-6&amp;partnerID=40&amp;md5=bb4117dd790d75c9fea41d1ab154724f', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1186/1471-2350-8-6\"\n     onclick=\"log_download('shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007', 'http://doi.org/10.1186/1471-2350-8-6', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shadrina-semenova-slominsky-bagyeva-illarioshkin-ivanovasmolenskaia-limborska-effectivequantitativerealtimepolymerasechainreactionanalysisoftheparkingenepark2exon112dosage-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shadrina2007,\r\nauthor={Shadrina, M.I. and Semenova, E.V. and Slominsky, P.A. and Bagyeva, G.H. and Illarioshkin, S.N. and Ivanova-Smolenskaia, I.I. and Limborska, S.A.},\r\ntitle={Effective quantitative real-time polymerase chain reaction analysis of the parkin gene (PARK2) exon 1-12 dosage},\r\njournal={BMC Medical Genetics},\r\nyear={2007},\r\nvolume={8},\r\ndoi={10.1186/1471-2350-8-6},\r\nart_number={6},\r\nnote={cited By 33},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847757812&amp;doi=10.1186%2f1471-2350-8-6&amp;partnerID=40&amp;md5=bb4117dd790d75c9fea41d1ab154724f},\r\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; Department of Neurogenetics, Institute of Neurology, 80 Volokolamskoye Highway, 123367 Moscow, Russian Federation},\r\nabstract={Background: One of the causes of Parkinson&#x27;s disease is mutations in the PARK2 gene. Deletions and duplications of single exons or exon groups account for a large proportion of the gene mutations. Direct detection of these mutations can be used for the diagnosis of Parkinson&#x27;s disease. Methods: To detect these mutations, we developed an effective technique based on the real-time TaqMan PCR system, which allows us to evaluate the copynumbers of the PARK2 gene exons by comparing the intensity of the amplification signals from some exon of this gene with that of the β-globin gene (the internal control). Results: We analyzed rearrangements in exons 1-12 of the PARK2 gene in 64 patients from Russia with early-onset Parkinson&#x27;s disease. The frequency of these mutations in our patients was 14%. Conclusion: We have developed a simple, accurate, and reproducible method applicable to the rapid detection of exon rearrangements in the PARK2 gene. It is suitable for the analysis of large patient groups, and it may become the basis for a diagnostic test. © 2007 Shadrina et al; licensee BioMed Central Ltd.},\r\ncorrespondence_address1={Semenova, E.V.; Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., 123182 Moscow, Russian Federation; email: semenova-helen@mail.ru},\r\nissn={14712350},\r\ncoden={BMGMA},\r\npubmed_id={17324265},\r\nlanguage={English},\r\nabbrev_source_title={BMC Med. Genet.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Background: One of the causes of Parkinson's disease is mutations in the PARK2 gene. Deletions and duplications of single exons or exon groups account for a large proportion of the gene mutations. Direct detection of these mutations can be used for the diagnosis of Parkinson's disease. Methods: To detect these mutations, we developed an effective technique based on the real-time TaqMan PCR system, which allows us to evaluate the copynumbers of the PARK2 gene exons by comparing the intensity of the amplification signals from some exon of this gene with that of the β-globin gene (the internal control). Results: We analyzed rearrangements in exons 1-12 of the PARK2 gene in 64 patients from Russia with early-onset Parkinson's disease. The frequency of these mutations in our patients was 14%. Conclusion: We have developed a simple, accurate, and reproducible method applicable to the rapid detection of exon rearrangements in the PARK2 gene. It is suitable for the analysis of large patient groups, and it may become the basis for a diagnostic test. © 2007 Shadrina et al; licensee BioMed Central Ltd.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&amp;doi=10.1083%2fjcb.200604167&amp;partnerID=40&amp;md5=b033b7ab6098f89f08e082e6f4d61463\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&amp;doi=10.1083%2fjcb.200604167&amp;partnerID=40&amp;md5=b033b7ab6098f89f08e082e6f4d61463', event);\">\n    Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shcherbakova, O.; Hurt, C.; Xiang, Y.; Dell'Acqua, M.; Zhang, Q.; Tsien, R.;  and Kobilka, B.\n</span>\n\n  \n\n</span>\n\n  <i>Journal of Cell Biology</i>, 176(4): 521-533.  2007.\n  <span class=\"note\">cited By 64</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&amp;doi=10.1083%2fjcb.200604167&amp;partnerID=40&amp;md5=b033b7ab6098f89f08e082e6f4d61463\"\n     onclick=\"log_download('shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&amp;doi=10.1083%2fjcb.200604167&amp;partnerID=40&amp;md5=b033b7ab6098f89f08e082e6f4d61463', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1083/jcb.200604167\"\n     onclick=\"log_download('shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007', 'http://doi.org/10.1083/jcb.200604167', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shcherbakova-hurt-xiang-dellacqua-zhang-tsien-kobilka-organizationofadrenoceptorsignalingcompartmentsbysympatheticinnervationofcardiacmyocytes-2007')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shcherbakova2007521,\r\nauthor={Shcherbakova, O.G. and Hurt, C.M. and Xiang, Y. and Dell&#x27;Acqua, M.L. and Zhang, Q. and Tsien, R.W. and Kobilka, B.K.},\r\ntitle={Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes},\r\njournal={Journal of Cell Biology},\r\nyear={2007},\r\nvolume={176},\r\nnumber={4},\r\npages={521-533},\r\ndoi={10.1083/jcb.200604167},\r\nnote={cited By 64},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846950037&amp;doi=10.1083%2fjcb.200604167&amp;partnerID=40&amp;md5=b033b7ab6098f89f08e082e6f4d61463},\r\naffiliation={Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; Department of Pharmacology, University of Colorado, Denver and Health Sciences Center, Aurora, CO 80045, United States; Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States},\r\nabstract={The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo. © The Rockefeller University Press.},\r\ncorrespondence_address1={Kobilka, B.K.; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 95305, United States; email: kobilka@stanford.edu},\r\nissn={00219525},\r\ncoden={JCLBA},\r\npubmed_id={17296797},\r\nlanguage={English},\r\nabbrev_source_title={J. Cell Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo. © The Rockefeller University Press.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2006\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2006')\"\n      onkeypress=\"toggleGroup('group_2006')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2006</span>\n      <span class=\"bibbase_group_count\">\n        \n        (6)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&amp;partnerID=40&amp;md5=3032bf178ce2c5b63c1c8996952a79f9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&amp;partnerID=40&amp;md5=3032bf178ce2c5b63c1c8996952a79f9', event);\">\n    Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shadrina, M.; Slominsky, P.; Zherebtsova, A.; Levitsky, G.; Levitskaya, N.; Alekhin, A.; Semenova, E.; Serdyuk, A.; Skvortsova, V.;  and Limborska, S.\n</span>\n\n  \n\n</span>\n\n  <i>Balkan Journal of Medical Genetics</i>, 9(1-2): 31-41.  2006.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&amp;partnerID=40&amp;md5=3032bf178ce2c5b63c1c8996952a79f9\"\n     onclick=\"log_download('shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&amp;partnerID=40&amp;md5=3032bf178ce2c5b63c1c8996952a79f9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shadrina-slominsky-zherebtsova-levitsky-levitskaya-alekhin-semenova-serdyuk-etal-detoxificationgenepolymorphismsandsusceptibilitytosporadicmotorneurondiseaseintherussianpopulation-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shadrina200631,\r\nauthor={Shadrina, M.I. and Slominsky, P.A. and Zherebtsova, A.L. and Levitsky, G.N. and Levitskaya, N.I. and Alekhin, A.V. and Semenova, E.V. and Serdyuk, A.V. and Skvortsova, V.L. and Limborska, S.A.},\r\ntitle={Detoxification gene polymorphisms and susceptibility to sporadic motor neuron disease in the Russian population},\r\njournal={Balkan Journal of Medical Genetics},\r\nyear={2006},\r\nvolume={9},\r\nnumber={1-2},\r\npages={31-41},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847047176&amp;partnerID=40&amp;md5=3032bf178ce2c5b63c1c8996952a79f9},\r\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; Department of Fundamental and Clinical Neurology, Russian State Medical University, Moscow, Russian Federation},\r\nabstract={Motor neuron disease (MND) results in selective degeneration of motor neurons of the cerebral cortex, brain stem and spinal cord. It is a complex disease in the pathogenesis of which many genetic systems may be involved. A significant etiological factor of MND may be oxygen free radicals which damage neuronal cells when present in high concentrations. In detoxification processes, free radicals may be formed that become transformed into non toxic products. The major participants of these processes are the cytochromes P-450 CYP2E1, CYP2D6, the glutathione-S-transferases GSTM1, GSTT1, GSTP1, and the N-acetyltransferase NAT2. To investigate their role in the development of MND, we have studied polymorphisms in these genes in 75 Russian patients with MND and 105 controls. We have established a statistical distinction in frequencies of CYP2E1*1D, and GSTM1 (0/0) homozygotes between the patient and the control samples. Whereas the analysis of the CYP2D6, GSTT1, GSTP1 and NAT2 gene polymorphisms has revealed no differences, the GSTP1*A/GSTP1*A genotype was associated with classical upper and lower motor neuron involvement. The GSTP1*B allele was associated predominantly with lower and upper motor neuron involvement. We propose that the genes of phases I and II of the detoxification system, CYP2E1, GSTP1, and GSTM1, participate in the development of sporadic MND in patients in Russia.},\r\nauthor_keywords={Detoxification processes;  Gene polymorphisms;  Motor neuron disease (MND)},\r\ncorrespondence_address1={Shadrina, M.I.; Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq.2, Moscow 123 182, Russian Federation; email: shadrina@img.ras.ru},\r\nissn={13110160},\r\ncoden={BJMGF},\r\nlanguage={English},\r\nabbrev_source_title={Balk. J. Med. Genet.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Motor neuron disease (MND) results in selective degeneration of motor neurons of the cerebral cortex, brain stem and spinal cord. It is a complex disease in the pathogenesis of which many genetic systems may be involved. A significant etiological factor of MND may be oxygen free radicals which damage neuronal cells when present in high concentrations. In detoxification processes, free radicals may be formed that become transformed into non toxic products. The major participants of these processes are the cytochromes P-450 CYP2E1, CYP2D6, the glutathione-S-transferases GSTM1, GSTT1, GSTP1, and the N-acetyltransferase NAT2. To investigate their role in the development of MND, we have studied polymorphisms in these genes in 75 Russian patients with MND and 105 controls. We have established a statistical distinction in frequencies of CYP2E1*1D, and GSTM1 (0/0) homozygotes between the patient and the control samples. Whereas the analysis of the CYP2D6, GSTT1, GSTP1 and NAT2 gene polymorphisms has revealed no differences, the GSTP1*A/GSTP1*A genotype was associated with classical upper and lower motor neuron involvement. The GSTP1*B allele was associated predominantly with lower and upper motor neuron involvement. We propose that the genes of phases I and II of the detoxification system, CYP2E1, GSTP1, and GSTM1, participate in the development of sporadic MND in patients in Russia.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&amp;doi=10.1016%2fj.neuropharm.2006.03.032&amp;partnerID=40&amp;md5=14ace5ce3f4045c0266da4c7f86c81d5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&amp;doi=10.1016%2fj.neuropharm.2006.03.032&amp;partnerID=40&amp;md5=14ace5ce3f4045c0266da4c7f86c81d5', event);\">\n    Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Brum, P.; Hurt, C.; Shcherbakova, O.; Kobilka, B.;  and Angelotti, T.\n</span>\n\n  \n\n</span>\n\n  <i>Neuropharmacology</i>, 51(3): 397-413.  2006.\n  <span class=\"note\">cited By 28</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&amp;doi=10.1016%2fj.neuropharm.2006.03.032&amp;partnerID=40&amp;md5=14ace5ce3f4045c0266da4c7f86c81d5\"\n     onclick=\"log_download('brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&amp;doi=10.1016%2fj.neuropharm.2006.03.032&amp;partnerID=40&amp;md5=14ace5ce3f4045c0266da4c7f86c81d5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.neuropharm.2006.03.032\"\n     onclick=\"log_download('brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006', 'http://doi.org/10.1016/j.neuropharm.2006.03.032', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('brum-hurt-shcherbakova-kobilka-angelotti-differentialtargetingandfunctionof2aand2cadrenergicreceptorsubtypesinculturedsympatheticneurons-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Brum2006397,\r\nauthor={Brum, P.C. and Hurt, C.M. and Shcherbakova, O.G. and Kobilka, B. and Angelotti, T.},\r\ntitle={Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons},\r\njournal={Neuropharmacology},\r\nyear={2006},\r\nvolume={51},\r\nnumber={3},\r\npages={397-413},\r\ndoi={10.1016/j.neuropharm.2006.03.032},\r\nnote={cited By 28},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747032525&amp;doi=10.1016%2fj.neuropharm.2006.03.032&amp;partnerID=40&amp;md5=14ace5ce3f4045c0266da4c7f86c81d5},\r\naffiliation={Laboratório de Fisiologia do Exercício, Escola de Educação Física e Esporte, Universidade de Sao Paulo, Av. Prof. Mello Moraes 65, 05508-900 Sao Paulo, SP, Brazil; Department of Molecular and Cellular Physiology, Stanford University Medical School, 157 Beckman Center, 279 Campus Drive, Stanford, CA 94305, United States; Department of Anesthesia, Stanford University Medical School, Stanford, CA 94305, United States},\r\nabstract={Previous research suggested that α2A and α2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed α2A and α2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, α2A and α2CARs could be readily detected in SGN from wild-type mice. By Day 8, α2AARs were targeted to cell body, as well as axonal and dendritic sites, whereas α2CARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, α2CARs had relocated to somatodendritic and axonal sites and, unlike α2AARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for α2ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous α2A and α2CAR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of α2CARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between α2A and α2CARs in SGN. © 2006 Elsevier Ltd. All rights reserved.},\r\nauthor_keywords={α2 adrenergic receptor;  Dexmedetomidine;  SV2;  Sympathetic ganglion neuron;  Trafficking},\r\nfunding_details={GM07365},\r\nfunding_details={98/14765-7},\r\n}</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Previous research suggested that α2A and α2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed α2A and α2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, α2A and α2CARs could be readily detected in SGN from wild-type mice. By Day 8, α2AARs were targeted to cell body, as well as axonal and dendritic sites, whereas α2CARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, α2CARs had relocated to somatodendritic and axonal sites and, unlike α2AARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for α2ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous α2A and α2CAR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of α2CARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between α2A and α2CARs in SGN. © 2006 Elsevier Ltd. All rights reserved.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&amp;partnerID=40&amp;md5=7eb6533d775986f49cbe70542290ec2d\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&amp;partnerID=40&amp;md5=7eb6533d775986f49cbe70542290ec2d', event);\">\n    The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Konevega, A.; Soboleva, N.; Makhno, V.; Peshekhonov, A.;  and Katunin, V.\n</span>\n\n  \n\n</span>\n\n  <i>Molekuliarnaia biologiia.</i>, 40(4): 669-683.  2006.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&amp;partnerID=40&amp;md5=7eb6533d775986f49cbe70542290ec2d\"\n     onclick=\"log_download('konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&amp;partnerID=40&amp;md5=7eb6533d775986f49cbe70542290ec2d', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('konevega-soboleva-makhno-peshekhonov-katunin-theeffectofmodificationoftrnanucleotide37onthetrnainteractionwiththepandasiteofthe70sribosomeescherichiacoli-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Konevega2006669,\r\nauthor={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Peshekhonov, A.V. and Katunin, V.I.},\r\ntitle={The effect of modification of tRNA nucleotide-37 on the tRNA interaction with the P- and A-site of the 70S ribosome Escherichia coli},\r\njournal={Molekuliarnaia biologiia.},\r\nyear={2006},\r\nvolume={40},\r\nnumber={4},\r\npages={669-683},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33749066025&amp;partnerID=40&amp;md5=7eb6533d775986f49cbe70542290ec2d},\r\nabstract={A modified nucleotide on the 3&#x27;-side of the anticodon loop of tRNA is one of the most important structure element regulating codon-anticodone interaction on the ribosome owing to the stacking interaction with the stack of codon-anticodon bases. The presence and identity (pyrimidine, purine or modified purine) of this nucleotide has an essential influence on the energy of the stacking interaction on A- and P-sites of the ribosome. There is a significant influence of the 37-modification by itself on the P-site, whereas there is no such one on the A-site of the ribosome. Comparison of binding enthalpies of tRNA interactions on the P- or A-site of the ribosome with the binding enthalpies of the complex of two tRNAs with the complementary anticodones suggests that the ribosome by itself significantly endows in the thermodynamics of codon-anticodon complex formation. It happens by additional ribosomal interactions with the molecule of tRNA or indirectly by the stabilization of codon-anticodon conformation. In addition to the stacking, tRNA binding in the A and P sites is futher stabilized by the interactions involving some magnesium ions. The number of them involved in those interactions strongly depends on the nucleotide identity in the 37-position of tRNA anticodon loop.},\r\ncorrespondence_address1={Konevega, A.L.},\r\nissn={00268984},\r\npubmed_id={16913226},\r\nlanguage={Russian},\r\nabbrev_source_title={Mol. Biol. (Mosk.)},\r\ndocument_type={Review},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A modified nucleotide on the 3'-side of the anticodon loop of tRNA is one of the most important structure element regulating codon-anticodone interaction on the ribosome owing to the stacking interaction with the stack of codon-anticodon bases. The presence and identity (pyrimidine, purine or modified purine) of this nucleotide has an essential influence on the energy of the stacking interaction on A- and P-sites of the ribosome. There is a significant influence of the 37-modification by itself on the P-site, whereas there is no such one on the A-site of the ribosome. Comparison of binding enthalpies of tRNA interactions on the P- or A-site of the ribosome with the binding enthalpies of the complex of two tRNAs with the complementary anticodones suggests that the ribosome by itself significantly endows in the thermodynamics of codon-anticodon complex formation. It happens by additional ribosomal interactions with the molecule of tRNA or indirectly by the stabilization of codon-anticodon conformation. In addition to the stacking, tRNA binding in the A and P sites is futher stabilized by the interactions involving some magnesium ions. The number of them involved in those interactions strongly depends on the nucleotide identity in the 37-position of tRNA anticodon loop.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&amp;doi=10.1134%2fS0026893306040121&amp;partnerID=40&amp;md5=68bb1ea3d0ae09fa1886bf15fc3f2c82\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&amp;doi=10.1134%2fS0026893306040121&amp;partnerID=40&amp;md5=68bb1ea3d0ae09fa1886bf15fc3f2c82', event);\">\n    Effect of modification of tRNA nucleotide 37 on the tRNA interaction with the A and P sites of the Escherichia coli 70S ribosome.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Molecular Biology</i>, 40(4): 597-610.  2006.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&amp;doi=10.1134%2fS0026893306040121&amp;partnerID=40&amp;md5=68bb1ea3d0ae09fa1886bf15fc3f2c82\"\n     onclick=\"log_download('anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747140413&amp;doi=10.1134%2fS0026893306040121&amp;partnerID=40&amp;md5=68bb1ea3d0ae09fa1886bf15fc3f2c82', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effect of modification of tRNA nucleotide 37 on the tRNA interaction with the A and P sites of the Escherichia coli 70S ribosome [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S0026893306040121\"\n     onclick=\"log_download('anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006', 'http://doi.org/10.1134/S0026893306040121', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-effectofmodificationoftrnanucleotide37onthetrnainteractionwiththeaandpsitesoftheescherichiacoli70sribosome-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The modified nucleotide 3′ of the tRNA anticodon is an important structural element that regulates the codon-anticodon interaction in the ribosome by stacking with codon-anticodon bases. The presence and identity (pyrimidine, purine, or modified purine) of this nucleotide significantly affects the energy of stacking in the A and P sites of the ribosome. Modification of nucleotide 37 does not contribute to stacking in the A site of the 70S ribosome, while its effect is substantial in the P site. The enthalpies of tRNA interactions with the A and P sites in the ribosome are similar and considerably lower than the enthalpy of the interactions of two tRNAs with the cognate anticodons in solution, suggesting that the ribosome contributes to the enthalpy-related portion of the free energy of tRNA binding by directly forming additional interactions with tRNA or by indirectly stabilizing the conformation of the codon-anticodon complex. In addition to stacking, tRNA binding in the A and P sites is further stabilized by interactions that involve magnesium ions. The number of ions involved in the formation of the tRNA-ribosome complex depends on the identity of tRNA nucleotide 37. © Pleiades Publishing, Inc., 2006.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&amp;partnerID=40&amp;md5=9d531826cec7c322a8080b0ec3f07a61\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&amp;partnerID=40&amp;md5=9d531826cec7c322a8080b0ec3f07a61', event);\">\n    Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Zherebtsova, A.; Shadrina, M.; Semenova, E.; Levitskiǐ, G.; Alekhin, A.; Slominskiǐ, P.; Skvortsova, V.;  and Limborskaia, S.\n</span>\n\n  \n\n</span>\n\n  <i>Genetika.</i>, 42(1): 104-109.  2006.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&amp;partnerID=40&amp;md5=9d531826cec7c322a8080b0ec3f07a61\"\n     onclick=\"log_download('zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&amp;partnerID=40&amp;md5=9d531826cec7c322a8080b0ec3f07a61', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('zherebtsova-shadrina-semenova-levitski-alekhin-slominski-skvortsova-limborskaia-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inpathogenesisofmotorneurondiseaseintherussianpopulation-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Zherebtsova2006104,\r\nauthor={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitskiǐ, G.N. and Alekhin, A.V. and Slominskiǐ, P.A. and Skvortsova, V.I. and Limborskaia, S.A.},\r\ntitle={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in pathogenesis of motor neuron disease in the Russian population},\r\njournal={Genetika.},\r\nyear={2006},\r\nvolume={42},\r\nnumber={1},\r\npages={104-109},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645834510&amp;partnerID=40&amp;md5=9d531826cec7c322a8080b0ec3f07a61},\r\nabstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND.},\r\ncorrespondence_address1={Zherebtsova, A.L.},\r\nissn={00166758},\r\npubmed_id={16523673},\r\nlanguage={Russian},\r\nabbrev_source_title={Genetika},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&amp;doi=10.1134%2fS1022795406010133&amp;partnerID=40&amp;md5=480e9bf567ea472b2cd6ccec20a9d4c5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&amp;doi=10.1134%2fS1022795406010133&amp;partnerID=40&amp;md5=480e9bf567ea472b2cd6ccec20a9d4c5', event);\">\n    Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Zherebtsova, A.; Shadrina, M.; Semenova, E.; Levitsky, G.; Alekhin, A.; Slominsky, P.; Skvortsova, V.;  and Limborska, S.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Genetics</i>, 42(1): 89-93.  2006.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&amp;doi=10.1134%2fS1022795406010133&amp;partnerID=40&amp;md5=480e9bf567ea472b2cd6ccec20a9d4c5\"\n     onclick=\"log_download('zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&amp;doi=10.1134%2fS1022795406010133&amp;partnerID=40&amp;md5=480e9bf567ea472b2cd6ccec20a9d4c5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1134/S1022795406010133\"\n     onclick=\"log_download('zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006', 'http://doi.org/10.1134/S1022795406010133', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('zherebtsova-shadrina-semenova-levitsky-alekhin-slominsky-skvortsova-limborska-analysisofthepossibleinvolvementoftheglutamatetransportergeneeaat2andtheglutamatereceptorgenesgria1andgria2inthepathogenesisofmotorneurondiseaseintherussianpopulation-2006')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Zherebtsova200689,\r\nauthor={Zherebtsova, A.L. and Shadrina, M.I. and Semenova, E.V. and Levitsky, G.N. and Alekhin, A.V. and Slominsky, P.A. and Skvortsova, V.I. and Limborska, S.A.},\r\ntitle={Analysis of the possible involvement of the glutamate transporter gene EAAT2 and the glutamate receptor genes GRIA1 and GRIA2 in the pathogenesis of motor neuron disease in the Russian population},\r\njournal={Russian Journal of Genetics},\r\nyear={2006},\r\nvolume={42},\r\nnumber={1},\r\npages={89-93},\r\ndoi={10.1134/S1022795406010133},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644639254&amp;doi=10.1134%2fS1022795406010133&amp;partnerID=40&amp;md5=480e9bf567ea472b2cd6ccec20a9d4c5},\r\naffiliation={Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russian Federation; Russian State Medical University, Moscow, 129327, Russian Federation},\r\nabstract={Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND. © Pleiades Publishing, Inc., 2006.},\r\nfunding_details={Российский Фонд Фундаментальных Исследований (РФФИ)04-04-49 124a, 02-04-49 076},\r\n}</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Polymorphisms of the genes of the glutamatergic system EAAT2, GRIA1, and GRIA2 have been analyzed in patients with sporadic motor neuron disease (MND) from Russia. The disease is not associated with polymorphic alleles of the genes studied, which indicates that EAAT2, GRIA1, and GRIA2 play an insignificant role in the pathogenesis of sporadic MND. © Pleiades Publishing, Inc., 2006.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2005\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2005')\"\n      onkeypress=\"toggleGroup('group_2005')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2005</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&amp;doi=10.1016%2fj.febslet.2005.01.052&amp;partnerID=40&amp;md5=3dd6734c8bbdb51d6d3bff6a1daf5aad\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&amp;doi=10.1016%2fj.febslet.2005.01.052&amp;partnerID=40&amp;md5=3dd6734c8bbdb51d6d3bff6a1daf5aad', event);\">\n    Fractal nature of chromatin organization in interphase chicken erythrocyte nuclei: DNA structure exhibits biphasic fractal properties.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>FEBS Letters</i>, 579(6): 1465-1468.  2005.\n  <span class=\"note\">cited By 59</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&amp;doi=10.1016%2fj.febslet.2005.01.052&amp;partnerID=40&amp;md5=3dd6734c8bbdb51d6d3bff6a1daf5aad\"\n     onclick=\"log_download('anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244270316&amp;doi=10.1016%2fj.febslet.2005.01.052&amp;partnerID=40&amp;md5=3dd6734c8bbdb51d6d3bff6a1daf5aad', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Fractal nature of chromatin organization in interphase chicken erythrocyte nuclei: DNA structure exhibits biphasic fractal properties [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/j.febslet.2005.01.052\"\n     onclick=\"log_download('anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005', 'http://doi.org/10.1016/j.febslet.2005.01.052', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-fractalnatureofchromatinorganizationininterphasechickenerythrocytenucleidnastructureexhibitsbiphasicfractalproperties-2005')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Arrangement of chromatin in intact chicken erythrocyte nuclei was investigated by small angle neutron scattering. The scattering spectra have revealed that on the scales between 15 nm and 1.5 μm the interior of the nucleus exhibited properties of a mass fractal. The fractal dimension of the protein component of cell nucleus held constant at approximately 2.5, while the DNA organization was biphasic, with the fractal dimension slightly higher than 2 on the scales smaller than 300 nm and approaching 3 on the larger scales. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2004\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2004')\"\n      onkeypress=\"toggleGroup('group_2004')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2004</span>\n      <span class=\"bibbase_group_count\">\n        \n        (2)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&amp;partnerID=40&amp;md5=448c2f76d3cf6675cc6b1eff278aefab\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&amp;partnerID=40&amp;md5=448c2f76d3cf6675cc6b1eff278aefab', event);\">\n    Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil'noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ivanov, A.; Bakhlanova, I.; Pantina, R.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiia</i>, 46(8): 740-747.  2004.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&amp;partnerID=40&amp;md5=448c2f76d3cf6675cc6b1eff278aefab\"\n     onclick=\"log_download('ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&amp;partnerID=40&amp;md5=448c2f76d3cf6675cc6b1eff278aefab', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil&#x27;noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ivanov-bakhlanova-pantina-filatov-instabilityofplasmiddnaintegratedintomammaliansomaticcellgenomeizuchenieiavleniianestabilnoiintegratsiichuzherodnoidnkvgenomsomaticheskikhkletokmlekopitaiushchikh-2004')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ivanov2004740,\r\nauthor={Ivanov, A.V. and Bakhlanova, I.V. and Pantina, R.A. and Filatov, M.V.},\r\ntitle={Instability of plasmid DNA integrated into mammalian somatic cell genome [Izuchenie iavleniia nestabil&#x27;noi integratsii chuzherodnoi DNK v genom somaticheskikh kletok mlekopitaiushchikh.]},\r\njournal={Tsitologiia},\r\nyear={2004},\r\nvolume={46},\r\nnumber={8},\r\npages={740-747},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-21644478726&amp;partnerID=40&amp;md5=448c2f76d3cf6675cc6b1eff278aefab},\r\nabstract={The phenomenon of loosing exogenic DNA from the mammalian somatic cell genome is under investigation. It is found that foreign DNA incorporated into cell genome as a result of transfection by electrophoretion may be lost with the frequency from 1/100 up to 1/100 000 per cell division during cultivation. This effect is not dependent of the nature of cell line and vector DNA. It is actual for different cell lines: A23, human fibroblasts AG 11395, murine embryonic line F9, and for different plasmid vectors: p16, p.39, pATR4 and pcDNA3.1-Higr (WRN). Integration of pDNA into genome and the following loosing of this DNA is registered by selection markers G418 and hygromycin B resistance and gancyclovir sensibility. The presence of foreign DNA in the genome was controlled by PCR. It is found that true foreign DNA deletion from the genome takes place rather than gene expression changes. For closely linked plasmid genes deletion of both genes at once as well as loosing any one gene separately is shown. Thus, the phenomenon of selective deletion of exogenic DNA from genome has been demonstrated for different mammalian cells.},\r\ncorrespondence_address1={Ivanov, A.V.},\r\nissn={00413771},\r\npubmed_id={15598021},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The phenomenon of loosing exogenic DNA from the mammalian somatic cell genome is under investigation. It is found that foreign DNA incorporated into cell genome as a result of transfection by electrophoretion may be lost with the frequency from 1/100 up to 1/100 000 per cell division during cultivation. This effect is not dependent of the nature of cell line and vector DNA. It is actual for different cell lines: A23, human fibroblasts AG 11395, murine embryonic line F9, and for different plasmid vectors: p16, p.39, pATR4 and pcDNA3.1-Higr (WRN). Integration of pDNA into genome and the following loosing of this DNA is registered by selection markers G418 and hygromycin B resistance and gancyclovir sensibility. The presence of foreign DNA in the genome was controlled by PCR. It is found that true foreign DNA deletion from the genome takes place rather than gene expression changes. For closely linked plasmid genes deletion of both genes at once as well as loosing any one gene separately is shown. Thus, the phenomenon of selective deletion of exogenic DNA from genome has been demonstrated for different mammalian cells.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&amp;doi=10.1261%2frna.5142404&amp;partnerID=40&amp;md5=c0ac9c400bbb3e2d3a57147153225962\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&amp;doi=10.1261%2frna.5142404&amp;partnerID=40&amp;md5=c0ac9c400bbb3e2d3a57147153225962', event);\">\n    Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Konevega, A.; Soboleva, N.; Makhno, V.; Semenkov, Y.; Wintermeyer, W.; Rodnina, M.;  and Katunin, V.\n</span>\n\n  \n\n</span>\n\n  <i>RNA</i>, 10(1): 90-101.  2004.\n  <span class=\"note\">cited By 73</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&amp;doi=10.1261%2frna.5142404&amp;partnerID=40&amp;md5=c0ac9c400bbb3e2d3a57147153225962\"\n     onclick=\"log_download('konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&amp;doi=10.1261%2frna.5142404&amp;partnerID=40&amp;md5=c0ac9c400bbb3e2d3a57147153225962', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1261/rna.5142404\"\n     onclick=\"log_download('konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004', 'http://doi.org/10.1261/rna.5142404', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('konevega-soboleva-makhno-semenkov-wintermeyer-rodnina-katunin-purinebasesatposition37oftrnastabilizecodonanticodoninteractionintheribosomalasitebystackingandmg2dependentinteractions-2004')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Konevega200490,\r\nauthor={Konevega, A.L. and Soboleva, N.G. and Makhno, V.I. and Semenkov, Y.P. and Wintermeyer, W. and Rodnina, M.V. and Katunin, V.I.},\r\ntitle={Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions},\r\njournal={RNA},\r\nyear={2004},\r\nvolume={10},\r\nnumber={1},\r\npages={90-101},\r\ndoi={10.1261/rna.5142404},\r\nnote={cited By 73},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347623198&amp;doi=10.1261%2frna.5142404&amp;partnerID=40&amp;md5=c0ac9c400bbb3e2d3a57147153225962},\r\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany; Institute of Physical Biochemistry, University of Witten/Herdecke, 58448 Witten, Germany},\r\nabstract={The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNAPhe binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNAPhe with hypermodified G (wybutine), or of unmodified peptidyl-tRNAPhe transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg 2+ ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg2+ -dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon-anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg2+ binding, and interactions with 16S rRNA.},\r\nauthor_keywords={Ribosome decoding;  Translation;  TRNA conformational change;  TRNA modifications;  TRNA selection},\r\ncorrespondence_address1={Katunin, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; email: katunin@omrb.pnpi.spb.ru},\r\nissn={13558382},\r\ncoden={RNARF},\r\npubmed_id={14681588},\r\nlanguage={English},\r\nabbrev_source_title={RNA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNAPhe binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNAPhe with hypermodified G (wybutine), or of unmodified peptidyl-tRNAPhe transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg 2+ ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg2+ -dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon-anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg2+ binding, and interactions with 16S rRNA.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2003\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2003')\"\n      onkeypress=\"toggleGroup('group_2003')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2003</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&amp;partnerID=40&amp;md5=5c8ad0bf4218457eab4cbe1a75ed3345\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&amp;partnerID=40&amp;md5=5c8ad0bf4218457eab4cbe1a75ed3345', event);\">\n    The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Soboleva, N.; Makhno, V.; Konevega, A.; Semenkov, Y.;  and Katunin, V.\n</span>\n\n  \n\n</span>\n\n  <i>Molekulyarnaya Biologiya</i>, 37(1): 121-127.  2003.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&amp;partnerID=40&amp;md5=5c8ad0bf4218457eab4cbe1a75ed3345\"\n     onclick=\"log_download('soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&amp;partnerID=40&amp;md5=5c8ad0bf4218457eab4cbe1a75ed3345', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('soboleva-makhno-konevega-semenkov-katunin-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Soboleva2003121,\r\nauthor={Soboleva, N.G. and Makhno, V.I. and Konevega, A.L. and Semenkov, Yu.P. and Katunin, V.I.},\r\ntitle={The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the a site of the 70S ribosome},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={2003},\r\nvolume={37},\r\nnumber={1},\r\npages={121-127},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037667672&amp;partnerID=40&amp;md5=5c8ad0bf4218457eab4cbe1a75ed3345},\r\naffiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},\r\nabstract={To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA K +Y Phe and Phe-tRNA K -Y Phe ) with the A site of complex [70S · poly(U) · deacylated tRNA Phe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA K +Y Phe showed, removal of the Y base decreased the association constant of Phe-tRNA K -Y Phe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA Phe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA K -Y Phe but not for Phe-tRNA K +Y Phe . Thus, the modified nucleotide 3′ of the Phe-tRNA Phe anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.},\r\ncorrespondence_address1={Soboleva, N.G.; Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad oblast, 188300, Russian Federation},\r\nissn={00268984},\r\ncoden={MOBIB},\r\npubmed_id={12624954},\r\nlanguage={Russian},\r\nabbrev_source_title={Mol. Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA K +Y Phe and Phe-tRNA K -Y Phe ) with the A site of complex [70S · poly(U) · deacylated tRNA Phe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA K +Y Phe showed, removal of the Y base decreased the association constant of Phe-tRNA K -Y Phe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA Phe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNA K -Y Phe but not for Phe-tRNA K +Y Phe . Thus, the modified nucleotide 3′ of the Phe-tRNA Phe anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&amp;doi=10.1261%2frna.2116303&amp;partnerID=40&amp;md5=b40491c4a5016c07d44c5c9308bbc767\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&amp;doi=10.1261%2frna.2116303&amp;partnerID=40&amp;md5=b40491c4a5016c07d44c5c9308bbc767', event);\">\n    Ribosomal localization of translation initiation factor IF2.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Marzi, S.; Knight, W.; Brandi, L.; Caserta, E.; Soboleva, N.; Hill, W.; Gualerzi, C.;  and Lodmell, J.\n</span>\n\n  \n\n</span>\n\n  <i>RNA</i>, 9(8): 958-969.  2003.\n  <span class=\"note\">cited By 43</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&amp;doi=10.1261%2frna.2116303&amp;partnerID=40&amp;md5=b40491c4a5016c07d44c5c9308bbc767\"\n     onclick=\"log_download('marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&amp;doi=10.1261%2frna.2116303&amp;partnerID=40&amp;md5=b40491c4a5016c07d44c5c9308bbc767', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Ribosomal localization of translation initiation factor IF2 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1261/rna.2116303\"\n     onclick=\"log_download('marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003', 'http://doi.org/10.1261/rna.2116303', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('marzi-knight-brandi-caserta-soboleva-hill-gualerzi-lodmell-ribosomallocalizationoftranslationinitiationfactorif2-2003')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Marzi2003958,\r\nauthor={Marzi, S. and Knight, W. and Brandi, L. and Caserta, E. and Soboleva, N. and Hill, W.E. and Gualerzi, C.O. and Lodmell, J.S.},\r\ntitle={Ribosomal localization of translation initiation factor IF2},\r\njournal={RNA},\r\nyear={2003},\r\nvolume={9},\r\nnumber={8},\r\npages={958-969},\r\ndoi={10.1261/rna.2116303},\r\nnote={cited By 43},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041806480&amp;doi=10.1261%2frna.2116303&amp;partnerID=40&amp;md5=b40491c4a5016c07d44c5c9308bbc767},\r\naffiliation={Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino (MC), Italy; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States},\r\nabstract={Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit&#x27;s factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.},\r\nauthor_keywords={BABE;  fMet-tRNA;  IF2;  Phenanthroline;  Ribosome topography;  Tethered nuclease;  Translation initiation},\r\ncorrespondence_address1={Lodmell, J.S.; Division of Biological Sciences, University of Montana, Missoula, MT 59812, United States; email: lodmell@selway.umt.edu},\r\nissn={13558382},\r\ncoden={RNARF},\r\npubmed_id={12869707},\r\nlanguage={English},\r\nabbrev_source_title={RNA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization of IF2 on the ribosomal subunits, a prerequisite for understanding the mechanism of initiation complex formation, has remained elusive. Here, we present a model for the positioning of IF2 in the 70S initiation complex as determined by cleavage of rRNA by the chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids in the GII domain of IF2 are in proximity to helices H3, H4, H17, and H18 of 16S rRNA. Furthermore, the junction of the C-1 and C-2 domains is in proximity to H89 and the thiostrepton region of 23S rRNA. The docking is further constrained by the requisite proximity of the C-2 domain with P-site-bound tRNA and by the conserved GI domain of the IF2 with the large subunit's factor-binding center. Comparison of our present findings with previous data further suggests that the IF2 orientation on the 30S subunit changes during the transition from the 30S to 70S initiation complex.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&amp;doi=10.1023%2fA%3a1022345115927&amp;partnerID=40&amp;md5=741954e32b6b93a44fe9c09521d9d1d0\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&amp;doi=10.1023%2fA%3a1022345115927&amp;partnerID=40&amp;md5=741954e32b6b93a44fe9c09521d9d1d0', event);\">\n    The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the a Site of the 70S Ribosome.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Molecular Biology</i>, 37(1): 110-115.  2003.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&amp;doi=10.1023%2fA%3a1022345115927&amp;partnerID=40&amp;md5=741954e32b6b93a44fe9c09521d9d1d0\"\n     onclick=\"log_download('anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037284145&amp;doi=10.1023%2fA%3a1022345115927&amp;partnerID=40&amp;md5=741954e32b6b93a44fe9c09521d9d1d0', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The Effect of Modification of Nucleotide-37 on the Interaction of Aminoacyl-tRNA with the a Site of the 70S Ribosome [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1023/A:1022345115927\"\n     onclick=\"log_download('anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003', 'http://doi.org/10.1023/A:1022345115927', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-theeffectofmodificationofnucleotide37ontheinteractionofaminoacyltrnawiththeasiteofthe70sribosome-2003')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  To estimate the effect of modified nucleotide 37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNA+YPhe and Phe-tRN A -YPhe) with the A site of complex [70S·poly(U) ·deacylated tRNAPhe in the P site] was assayed at 0-20°C. As comparisons with native Phe-tRNA+YPhe showed, removal of the Y base decreased the association constant of Phe-tRN A-Y Phe and the complex by an order of magnitude at every temperature tested, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNAPhe bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRN A-YPhe but not for Phe-tRN A +YPhe. Thus, the modified nucleotide 3′ of the Phe-tRNAPhe anticodon stabilized the codon-anticodon interaction both in the A and P sites of the 70S ribosome.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2002\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2002')\"\n      onkeypress=\"toggleGroup('group_2002')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2002</span>\n      <span class=\"bibbase_group_count\">\n        \n        (4)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&amp;partnerID=40&amp;md5=6cd83eb630a9b3ded1792a7091a98ebf\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&amp;partnerID=40&amp;md5=6cd83eb630a9b3ded1792a7091a98ebf', event);\">\n    The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Drobchenko, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 44(5): 475-476.  2002.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&amp;partnerID=40&amp;md5=6cd83eb630a9b3ded1792a7091a98ebf\"\n     onclick=\"log_download('semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&amp;partnerID=40&amp;md5=6cd83eb630a9b3ded1792a7091a98ebf', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-drobchenko-filatov-thecriterionofflowcitometricalestimationofthenuclearchromatinstateinhumanspermatozoausedasatestfortheirfertility-2002')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova2002475,\r\nauthor={Semenova, E.V. and Drobchenko, E.A. and Filatov, M.V.},\r\ntitle={The criterion of flow citometrical estimation of the nuclear chromatin state in human spermatozoa used as a test for their fertility},\r\njournal={Tsitologiya},\r\nyear={2002},\r\nvolume={44},\r\nnumber={5},\r\npages={475-476},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041878934&amp;partnerID=40&amp;md5=6cd83eb630a9b3ded1792a7091a98ebf},\r\naffiliation={St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation},\r\nabstract={On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.},\r\ncorrespondence_address1={Semenova, E.V.; St. Petersburg Nucl. Phys. Inst. RAS, St. Petersburg, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\r\nissn={00413771},\r\ncoden={TSITA},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  On the basis of the previously described method for estimating the state of the human spermatozoan nuclear chromatin by flow cytometry, we developed a modified system of calculation and presentation of our data. We propose a criterion that allows to classify samples of seminal material according to their fertility potential that could be used for male sterility diagnostics and as a prognostic test for optimization of reproduction programs. The most important theoretical and methodical aspects of the suggested approach are discussed, and comparative analysis of suggested approach and routine flow cytometry SCSA method is made.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&amp;doi=10.1023%2fA%3a1016283005773&amp;partnerID=40&amp;md5=688457cdf35a3f7a28492b01e8879a50\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&amp;doi=10.1023%2fA%3a1016283005773&amp;partnerID=40&amp;md5=688457cdf35a3f7a28492b01e8879a50', event);\">\n    Maleic acid utilization by mixed cultures of microorganisms.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Applied Biochemistry and Microbiology</i>, 38(4): 346-348.  2002.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&amp;doi=10.1023%2fA%3a1016283005773&amp;partnerID=40&amp;md5=688457cdf35a3f7a28492b01e8879a50\"\n     onclick=\"log_download('safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&amp;doi=10.1023%2fA%3a1016283005773&amp;partnerID=40&amp;md5=688457cdf35a3f7a28492b01e8879a50', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Maleic acid utilization by mixed cultures of microorganisms [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1023/A:1016283005773\"\n     onclick=\"log_download('safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002', 'http://doi.org/10.1023/A:1016283005773', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova2002346,\r\nauthor={Safronova, I.Yu. and Semenova, E.V.},\r\ntitle={Maleic acid utilization by mixed cultures of microorganisms},\r\njournal={Applied Biochemistry and Microbiology},\r\nyear={2002},\r\nvolume={38},\r\nnumber={4},\r\npages={346-348},\r\ndoi={10.1023/A:1016283005773},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141607907&amp;doi=10.1023%2fA%3a1016283005773&amp;partnerID=40&amp;md5=688457cdf35a3f7a28492b01e8879a50},\r\naffiliation={Biological Faculty, Moscow State University, Vorob&#x27;evy gory, Moscow, 119899, Russian Federation},\r\nabstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp, xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and B. subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions of water.},\r\ncorrespondence_address1={Safronova, I.Yu.; Biological Faculty, Moscow State University, Vorob&#x27;evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},\r\nissn={00036838},\r\nlanguage={English},\r\nabbrev_source_title={Appl. Biochem. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  In a mixed batch culture, Alcaligenes xylosoxidans subsp, xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and B. subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions of water.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&amp;partnerID=40&amp;md5=6a3d91e3788a2afd0731b1467fd0e5a9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&amp;partnerID=40&amp;md5=6a3d91e3788a2afd0731b1467fd0e5a9', event);\">\n    A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing».\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Levina, V.; Drobchenko, E.; Sukhareva, E.; Shabalina, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 44(6): 574-575.  2002.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&amp;partnerID=40&amp;md5=6a3d91e3788a2afd0731b1467fd0e5a9\"\n     onclick=\"log_download('levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&amp;partnerID=40&amp;md5=6a3d91e3788a2afd0731b1467fd0e5a9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing» [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('levina-drobchenko-sukhareva-shabalina-filatov-acrossdrugresistanceofmammaliancellswithahighefficiencyofdnaclearing-2002')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Levina2002574,\r\nauthor={Levina, V.V. and Drobchenko, E.A. and Sukhareva, E.B. and Shabalina, E.V. and Filatov, M.V.},\r\ntitle={A cross drug resistance of mammalian cells with a high efficiency of «DNA clearing»},\r\njournal={Tsitologiya},\r\nyear={2002},\r\nvolume={44},\r\nnumber={6},\r\npages={574-575},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-2242471181&amp;partnerID=40&amp;md5=6a3d91e3788a2afd0731b1467fd0e5a9},\r\nabstract={The process of active dissociation of noncovalently bound agents from DNA or «DNA clearing» in the living cells was described elsewhere. The vital fluorescent bisbenzimidazole dye Hoechst 33342 (4342), which binds tightly but not covalently to DNA in the minor groove, was used for studying interactions of agents noncovalently binding with DNA. The «DNA clearing» is an energy-dependent process, which is suppressed by topoisomerase-II inhibitors and DNA breaks. It has been shown that the rodent fibroblast cell line AA8HoeR-7 is selected for resistance to H342, and characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. This cell line obtained cross-resistance to other DNA damaging drugs: mitomycin C, etoposide and ethidium bromide. That proves that AA8HoeR-7 is cell line with a new mechanism of multidrug resistance.},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={12236101},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The process of active dissociation of noncovalently bound agents from DNA or «DNA clearing» in the living cells was described elsewhere. The vital fluorescent bisbenzimidazole dye Hoechst 33342 (4342), which binds tightly but not covalently to DNA in the minor groove, was used for studying interactions of agents noncovalently binding with DNA. The «DNA clearing» is an energy-dependent process, which is suppressed by topoisomerase-II inhibitors and DNA breaks. It has been shown that the rodent fibroblast cell line AA8HoeR-7 is selected for resistance to H342, and characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. This cell line obtained cross-resistance to other DNA damaging drugs: mitomycin C, etoposide and ethidium bromide. That proves that AA8HoeR-7 is cell line with a new mechanism of multidrug resistance.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&amp;partnerID=40&amp;md5=fad79a8ef19fa76e2130fd1bdce1e62c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&amp;partnerID=40&amp;md5=fad79a8ef19fa76e2130fd1bdce1e62c', event);\">\n    Maleic Acid Utilization by Mixed Cultures of Microorganisms.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Prikladnaya Biokhimiya i Mikrobiologiya</i>, 38(4): 403-404.  2002.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&amp;partnerID=40&amp;md5=fad79a8ef19fa76e2130fd1bdce1e62c\"\n     onclick=\"log_download('safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&amp;partnerID=40&amp;md5=fad79a8ef19fa76e2130fd1bdce1e62c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Maleic Acid Utilization by Mixed Cultures of Microorganisms [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-maleicacidutilizationbymixedculturesofmicroorganisms-2002')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova2002403,\r\nauthor={Safronova, I.Yu. and Semenova, E.V.},\r\ntitle={Maleic Acid Utilization by Mixed Cultures of Microorganisms},\r\njournal={Prikladnaya Biokhimiya i Mikrobiologiya},\r\nyear={2002},\r\nvolume={38},\r\nnumber={4},\r\npages={403-404},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346438845&amp;partnerID=40&amp;md5=fad79a8ef19fa76e2130fd1bdce1e62c},\r\naffiliation={Moscow State University, Biological Faculty, Vorob&#x27;evy gory, Moscow, 119899, Russian Federation},\r\nabstract={In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.},\r\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Biological Faculty, Vorob&#x27;evy gory, Moscow, 119899, Russian Federation; email: samsevsva@mtu-net.ru},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={05551099},\r\npubmed_id={12325296},\r\nlanguage={Russian},\r\nabbrev_source_title={Prikl. Biokhim. Mikrobiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  In a mixed batch culture, Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed maleic acid into malic acid. Bacillus subtilis 271 used malic acid as a substrate, thus stimulating further transformation of maleic acid. Both bacterial cultures dissociated with the formation of R, S, and M forms. At a concentration of 5.0 g/l, maleic acid was utilized maximally by RS and SS forms of the association A. xylosoxidans and Bacillus subtilis. At concentrations 15.0 and 25.0 g/l, maleic acid was utilized maximally by SS and MS forms of the mixed culture, respectively. Association of bacteria A. xylosoxidans and B. subtilis was not stable under flow conditions water.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2001\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2001')\"\n      onkeypress=\"toggleGroup('group_2001')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2001</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&amp;doi=10.1023%2fA%3a1010245903543&amp;partnerID=40&amp;md5=a99a82099320d937b20e7b2a4432d13c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&amp;doi=10.1023%2fA%3a1010245903543&amp;partnerID=40&amp;md5=a99a82099320d937b20e7b2a4432d13c', event);\">\n    Optimum conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Applied Biochemistry and Microbiology</i>, 37(4): 374-375.  2001.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&amp;doi=10.1023%2fA%3a1010245903543&amp;partnerID=40&amp;md5=a99a82099320d937b20e7b2a4432d13c\"\n     onclick=\"log_download('safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&amp;doi=10.1023%2fA%3a1010245903543&amp;partnerID=40&amp;md5=a99a82099320d937b20e7b2a4432d13c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Optimum conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1023/A:1010245903543\"\n     onclick=\"log_download('safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001', 'http://doi.org/10.1023/A:1010245903543', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-optimumconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspxylosoxidans260-2001')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova2001374,\r\nauthor={Safronova, I.Yu. and Semenova, E.V.},\r\ntitle={Optimum conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260},\r\njournal={Applied Biochemistry and Microbiology},\r\nyear={2001},\r\nvolume={37},\r\nnumber={4},\r\npages={374-375},\r\ndoi={10.1023/A:1010245903543},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27144522144&amp;doi=10.1023%2fA%3a1010245903543&amp;partnerID=40&amp;md5=a99a82099320d937b20e7b2a4432d13c},\r\naffiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\r\nabstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed 98% of the maleic acid (initial concentration of 5.0 g/l medium) under periodic conditions for 48 h. Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans transformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h-1. © 2001 MAIK &quot;Nauka/Interperiodica&quot;.},\r\ncorrespondence_address1={Safronova, I.Yu.; Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\r\npublisher={Kluwer Academic/Plenum Publishers},\r\nissn={00036838},\r\nlanguage={English},\r\nabbrev_source_title={Appl. Biochem. Microbiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 transformed 98% of the maleic acid (initial concentration of 5.0 g/l medium) under periodic conditions for 48 h. Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans transformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h-1. © 2001 MAIK \"Nauka/Interperiodica\".\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&amp;partnerID=40&amp;md5=9ce3e167f5fe2d61369252ced3a4fd9f\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&amp;partnerID=40&amp;md5=9ce3e167f5fe2d61369252ced3a4fd9f', event);\">\n    Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Prikladnaia biokhimiia i mikrobiologiia</i>, 37(4): 436-438.  2001.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&amp;partnerID=40&amp;md5=9ce3e167f5fe2d61369252ced3a4fd9f\"\n     onclick=\"log_download('safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&amp;partnerID=40&amp;md5=9ce3e167f5fe2d61369252ced3a4fd9f', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-optimizationofconditionsfortransformationofmaleicacidbyimmobilizedcellsofalcaligenesxylosoxidanssubspeciesxylosoxidans260optimizatsiiausloviitransformatsiimaleinovoikislotypriimmobilizatsiikletokalcaligenesxylosoxidanssubspxylosoxidans260-2001')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova2001436,\r\nauthor={Safronova, I.I. and Semenova, E.V.},\r\ntitle={Optimization of conditions for transformation of maleic acid by immobilized cells of Alcaligenes xylosoxidans subspecies xylosoxidans 260 [Optimizatsiia uslovii transformatsii maleinovoi kisloty pri immobilizatsii kletok Alcaligenes xylosoxidans subsp. xylosoxidans 260.]},\r\njournal={Prikladnaia biokhimiia i mikrobiologiia},\r\nyear={2001},\r\nvolume={37},\r\nnumber={4},\r\npages={436-438},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035404782&amp;partnerID=40&amp;md5=9ce3e167f5fe2d61369252ced3a4fd9f},\r\naffiliation={Lomonosov Moscow State University, Biological Department, Moscow, Russian Federation},\r\nabstract={Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.},\r\ncorrespondence_address1={Safronova, I.I.},\r\nissn={05551099},\r\npubmed_id={11530667},\r\nlanguage={Russian},\r\nabbrev_source_title={Prikl. Biokhim. Mikrobiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Immobilized cells of Alcaligenes xylosoxidans subsp. xylosoxidans 260 used 98% of maleic acid (initial concentration of 5.0 g/l medium) in periodic conditions for 48 h. Free cells transformed only 26% of substrate in 96 h. Immobilized cells of a selected S-variant of A. xylosoxidans used maleate (30.0 g/l) entirely in 96 h during periodic cultivation and only 15.0 g/l of maleate in continuous cultivation at a flow rate of 0.03 h-1.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&amp;partnerID=40&amp;md5=9183e57df4f31e5ae6a0b50434f95a09\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&amp;partnerID=40&amp;md5=9183e57df4f31e5ae6a0b50434f95a09', event);\">\n    Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral'noi nervnoi sistemy mogut byt' dostupny dlia ataki spetsificheskimi antitelami.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Blizniukov, O.; Kozmin, L.; Oliushin, V.; Ostreiko, O.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Voprosy onkologii</i>, 47(1): 49-51.  2001.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&amp;partnerID=40&amp;md5=9183e57df4f31e5ae6a0b50434f95a09\"\n     onclick=\"log_download('blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&amp;partnerID=40&amp;md5=9183e57df4f31e5ae6a0b50434f95a09', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral&#x27;noi nervnoi sistemy mogut byt&#x27; dostupny dlia ataki spetsificheskimi antitelami.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('blizniukov-kozmin-oliushin-ostreiko-filatov-malignantcentralnervoussystemtumorsaresusceptibletospecificantibodieszlokachestvennyeopukholitsentralnoinervnoisistemymogutbytdostupnydliaatakispetsificheskimiantitelami-2001')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Blizniukov200149,\r\nauthor={Blizniukov, O.P. and Kozmin, L.D. and Oliushin, V.E. and Ostreiko, O.V. and Filatov, M.V.},\r\ntitle={Malignant central nervous system tumors are susceptible to specific antibodies [Zlokachestvennye opukholi tsentral&#x27;noi nervnoi sistemy mogut byt&#x27; dostupny dlia ataki spetsificheskimi antitelami.]},\r\njournal={Voprosy onkologii},\r\nyear={2001},\r\nvolume={47},\r\nnumber={1},\r\npages={49-51},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035221713&amp;partnerID=40&amp;md5=9183e57df4f31e5ae6a0b50434f95a09},\r\naffiliation={St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russian Federation},\r\nabstract={The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.},\r\ncorrespondence_address1={Blizniukov, O.P.},\r\nissn={05073758},\r\npubmed_id={11317535},\r\nlanguage={Russian},\r\nabbrev_source_title={Vopr Onkol},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The binding of circulating specific IgG to glioblastoma cells from brain tumor biopsies was shown using fluorescence conjugate Protein A-FITC and Western blotting. Blood-brain barrier permeability for antitumor antibodies in vivo in glioblastoma patients is suggested.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_2000\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_2000')\"\n      onkeypress=\"toggleGroup('group_2000')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>2000</span>\n      <span class=\"bibbase_group_count\">\n        \n        (9)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&amp;partnerID=40&amp;md5=2af0b62eb2bdcdb6f93bb91fdd52b885\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&amp;partnerID=40&amp;md5=2af0b62eb2bdcdb6f93bb91fdd52b885', event);\">\n    Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shcherbakova, O.; Lantsov, V.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Doklady Akademii Nauk</i>, 371(6): 829-833.  2000.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&amp;partnerID=40&amp;md5=2af0b62eb2bdcdb6f93bb91fdd52b885\"\n     onclick=\"log_download('shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&amp;partnerID=40&amp;md5=2af0b62eb2bdcdb6f93bb91fdd52b885', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shcherbakova-lantsov-filatov-overexpressionofbacterialrecaproteininmammaliancellsincreasesthefrequencyofgenetargeting-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shcherbakova2000829,\r\nauthor={Shcherbakova, O.G. and Lantsov, V.A. and Filatov, M.V.},\r\ntitle={Overexpression of bacterial recA protein in mammalian cells increases the frequency of gene targeting},\r\njournal={Doklady Akademii Nauk},\r\nyear={2000},\r\nvolume={371},\r\nnumber={6},\r\npages={829-833},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748964627&amp;partnerID=40&amp;md5=2af0b62eb2bdcdb6f93bb91fdd52b885},\r\nissn={08695652},\r\ncoden={DAKNE},\r\nlanguage={Russian},\r\nabbrev_source_title={Dokl. Akad. Nauk},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&amp;partnerID=40&amp;md5=fa59c80a48a0c518643554b7cbd2df52\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&amp;partnerID=40&amp;md5=fa59c80a48a0c518643554b7cbd2df52', event);\">\n    A family öf shuttle vectors 'for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Aleshin, V.; Semenova, E.; Tarakanov, B.;  and Livshits, V.\n</span>\n\n  \n\n</span>\n\n  <i>Mikrobiologiya</i>, 69(1): 75-80.  2000.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&amp;partnerID=40&amp;md5=fa59c80a48a0c518643554b7cbd2df52\"\n     onclick=\"log_download('aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&amp;partnerID=40&amp;md5=fa59c80a48a0c518643554b7cbd2df52', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A family öf shuttle vectors &#x27;for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('aleshin-semenova-tarakanov-livshits-afamilyfshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Aleshin200075,\r\nauthor={Aleshin, V.V. and Semenova, E.V. and Tarakanov, B.V. and Livshits, V.A.},\r\ntitle={A family öf shuttle vectors &#x27;for lactic acid bacteria and other gram-positive bacteria based on the plasmid Plf1311 replicon},\r\njournal={Mikrobiologiya},\r\nyear={2000},\r\nvolume={69},\r\nnumber={1},\r\npages={75-80},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033656978&amp;partnerID=40&amp;md5=fa59c80a48a0c518643554b7cbd2df52},\r\naffiliation={All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},\r\nabstract={A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in grampositive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc,) was created. The vectors are based on the cryptic plasmid pLFOll uom&#x27;Lactoimcillus fermentum VKM 1311 belonging to a family.of the o-type pE194-like plasmkls. The vectors can replicite in .gram-positive bacteria and Escherichia coll, &quot;They, are stable in many gram-positive bacteria, &quot;have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that&#x27;contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPot can&#x27; be mobilized in a great number&#x27; of bacteria using a&#x27; helper pltlmid from E, coll but not from1 gram-positive bacteria,.},\r\nauthor_keywords={Gram-positive bacteria;  Lactic acid bacteria;  Shuttle vectors;  Ss-dna plasmids},\r\ncorrespondence_address1={Aleshin, V.V.; All-Rmsm Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, 249010, Russian Federation},\r\nissn={00263656},\r\ncoden={MIKBA},\r\npubmed_id={10808493},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikrobiologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in grampositive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc,) was created. The vectors are based on the cryptic plasmid pLFOll uom'Lactoimcillus fermentum VKM 1311 belonging to a family.of the o-type pE194-like plasmkls. The vectors can replicite in .gram-positive bacteria and Escherichia coll, \"They, are stable in many gram-positive bacteria, \"have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that'contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPot can' be mobilized in a great number' of bacteria using a' helper pltlmid from E, coll but not from1 gram-positive bacteria,.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&amp;partnerID=40&amp;md5=c8abc6251cfd7f665b4becca652d030a\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&amp;partnerID=40&amp;md5=c8abc6251cfd7f665b4becca652d030a', event);\">\n    Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel'nyi stress pri hypertonicheskikh krizakh.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Golikov, P.; Davydov, B.; Marchenko, V.; Nikolaeva, N.; Golikov, A.; Riabinin, V.; Semenova, E.;  and Polumiskov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Klinicheskaya Meditsina</i>, 78(7): 42-45.  2000.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&amp;partnerID=40&amp;md5=c8abc6251cfd7f665b4becca652d030a\"\n     onclick=\"log_download('golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&amp;partnerID=40&amp;md5=c8abc6251cfd7f665b4becca652d030a', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel&#x27;nyi stress pri hypertonicheskikh krizakh.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('golikov-davydov-marchenko-nikolaeva-golikov-riabinin-semenova-polumiskov-urapidileffectsonoxidativestressinhypertensivecrisesvliianieurapidilanaokislitelnyistressprihypertonicheskikhkrizakh-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Golikov200042,\r\nauthor={Golikov, P.P. and Davydov, B.V. and Marchenko, V.V. and Nikolaeva, N.I. and Golikov, A.P. and Riabinin, V.A. and Semenova, E.V. and Polumiskov, V.I.},\r\ntitle={Urapidil effects on oxidative stress in hypertensive crises [Vliianie urapidila na okislitel&#x27;nyi stress pri hypertonicheskikh krizakh.]},\r\njournal={Klinicheskaya Meditsina},\r\nyear={2000},\r\nvolume={78},\r\nnumber={7},\r\npages={42-45},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033648479&amp;partnerID=40&amp;md5=c8abc6251cfd7f665b4becca652d030a},\r\nabstract={Urapidil effects on oxidant stress were studied in 36 patients with hypertension stage I-III running with crises. Acute hypertensive crisis was managed by intravenous injections of urapidil (20-50 mg) for 5 min. After that for a week they received urapidil monotherapy followed by combined treatment for another week (quinapril--25 mg/day or ramipril--5-10 mg/day in combination with hypothiaside--25 mg/day; lokren--20 mg/day or atenolol--50-100 mg/day and diltiazem--180-360 mg/day) to stabilize arterial pressure finally. Oxidant stress was estimated by lipid peroxidation (LPO) and state of antioxidant system (AOS). Catabolism and anabolism were evaluated by blood levels of hydrocortisone and insulin. LPO, AOS, the blood hormones were measured before management of hypertensive crisis, immediately after it and 1, 3, 7 and 14 days after. Before the crisis treatment LPO was found high. Urapidil administration reduced levels of hydrocortisone, dienic conjugates, lipid peroxidation, oxidant stress rate (OSR). Later, LPO and OSR slowly increased. It is concluded on validity of the antioxidants use in hypertensive patients with crises.},\r\ncorrespondence_address1={Golikov, P.P.},\r\nissn={00232149},\r\npubmed_id={10979642},\r\nlanguage={Russian},\r\nabbrev_source_title={Klin Med (Mosk)},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Urapidil effects on oxidant stress were studied in 36 patients with hypertension stage I-III running with crises. Acute hypertensive crisis was managed by intravenous injections of urapidil (20-50 mg) for 5 min. After that for a week they received urapidil monotherapy followed by combined treatment for another week (quinapril–25 mg/day or ramipril–5-10 mg/day in combination with hypothiaside–25 mg/day; lokren–20 mg/day or atenolol–50-100 mg/day and diltiazem–180-360 mg/day) to stabilize arterial pressure finally. Oxidant stress was estimated by lipid peroxidation (LPO) and state of antioxidant system (AOS). Catabolism and anabolism were evaluated by blood levels of hydrocortisone and insulin. LPO, AOS, the blood hormones were measured before management of hypertensive crisis, immediately after it and 1, 3, 7 and 14 days after. Before the crisis treatment LPO was found high. Urapidil administration reduced levels of hydrocortisone, dienic conjugates, lipid peroxidation, oxidant stress rate (OSR). Later, LPO and OSR slowly increased. It is concluded on validity of the antioxidants use in hypertensive patients with crises.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&amp;partnerID=40&amp;md5=70cc6ef737ebc7edab2a5f3f4dcc9536\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&amp;partnerID=40&amp;md5=70cc6ef737ebc7edab2a5f3f4dcc9536', event);\">\n    A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Levina, V.; Varfolomeeva, E.; Sukhareva, E.; Shabalina, E.; Drobchenko, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>ATLA Alternatives to Laboratory Animals</i>, 28(3): 451-456.  2000.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&amp;partnerID=40&amp;md5=70cc6ef737ebc7edab2a5f3f4dcc9536\"\n     onclick=\"log_download('levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&amp;partnerID=40&amp;md5=70cc6ef737ebc7edab2a5f3f4dcc9536', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('levina-varfolomeeva-sukhareva-shabalina-drobchenko-filatov-anewmechanismofmammaliancellresistancetothetoxiceffectsofdnabindingagents-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Levina2000451,\r\nauthor={Levina, V. and Varfolomeeva, E. and Sukhareva, E. and Shabalina, E. and Drobchenko, E. and Filatov, M.},\r\ntitle={A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents},\r\njournal={ATLA Alternatives to Laboratory Animals},\r\nyear={2000},\r\nvolume={28},\r\nnumber={3},\r\npages={451-456},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034122335&amp;partnerID=40&amp;md5=70cc6ef737ebc7edab2a5f3f4dcc9536},\r\naffiliation={Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},\r\nabstract={The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step- by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-1-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33342 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.},\r\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Mitomycin C;  Multidrug resistance},\r\ncorrespondence_address1={Levina, V.; Petersburg Nuclear Physics Institute, Gatchina, St Petersburg 188350, Russian Federation},\r\nissn={02611929},\r\ncoden={AALAD},\r\nlanguage={English},\r\nabbrev_source_title={ATLA Altern. Lab. Anim.},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step- by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-1-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33342 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&amp;doi=10.1016%2fS0921-8777%2899%2900059-2&amp;partnerID=40&amp;md5=e702eb119dd51b6527762dbac2a88b00\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&amp;doi=10.1016%2fS0921-8777%2899%2900059-2&amp;partnerID=40&amp;md5=e702eb119dd51b6527762dbac2a88b00', event);\">\n    Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Mutation Research - DNA Repair</i>, 459(1): 65-71.  2000.\n  <span class=\"note\">cited By 30</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&amp;doi=10.1016%2fS0921-8777%2899%2900059-2&amp;partnerID=40&amp;md5=e702eb119dd51b6527762dbac2a88b00\"\n     onclick=\"log_download('anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033951981&amp;doi=10.1016%2fS0921-8777%2899%2900059-2&amp;partnerID=40&amp;md5=e702eb119dd51b6527762dbac2a88b00', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Overexpression of bacterial RecA protein stimulates homologous recombination in somatic mammalian cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/S0921-8777(99)00059-2\"\n     onclick=\"log_download('anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000', 'http://doi.org/10.1016/S0921-8777(99)00059-2', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-overexpressionofbacterialrecaproteinstimulateshomologousrecombinationinsomaticmammaliancells-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The pairing of homologous molecules and strand exchange is a key event in homologous recombination promoted by RecA protein in Escherichia coli. Structural homologs of RecA are widely distributed in eukaryotes including mouse and man. As has been shown, human HsRad51 protein is not only structural but also functional homolog of RecA. The question arises whether the bacterial functional homolog of Rad51 can function in mammalian cells and increase the frequency of the homologous recombination. To investigate possible effects of bacterial RecA protein on the frequency of homologous recombination in mammalian cells, the E. coli RecA protein fused with a nuclear location signal from the large T antigen of simian virus 40 was overexpressed in the mouse F9 teratocarcinoma cells. We found that the frequency of gene targeting at the hprt locus was 10-fold increased in the mouse cells expressing the nucleus-targeted RecA protein. Southern blot analysis of individual clones that were generated by targeting recombination revealed predicted type of alterations in hprt gene. The data indicate that the bacterial nucleus-targeted RecA protein can stimulate homologous recombination in mammalian cells. Copyright (C) 2000 Elsevier Science B.V.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&amp;doi=10.1016%2fS0167-4889%2899%2900151-2&amp;partnerID=40&amp;md5=da5b2e1f4286e2c7e96d04eac53c4b64\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&amp;doi=10.1016%2fS0167-4889%2899%2900151-2&amp;partnerID=40&amp;md5=da5b2e1f4286e2c7e96d04eac53c4b64', event);\">\n    Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shcherbakova, O.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Biochimica et Biophysica Acta - Molecular Cell Research</i>, 1495(1): 1-3.  2000.\n  <span class=\"note\">cited By 10</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&amp;doi=10.1016%2fS0167-4889%2899%2900151-2&amp;partnerID=40&amp;md5=da5b2e1f4286e2c7e96d04eac53c4b64\"\n     onclick=\"log_download('shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&amp;doi=10.1016%2fS0167-4889%2899%2900151-2&amp;partnerID=40&amp;md5=da5b2e1f4286e2c7e96d04eac53c4b64', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/S0167-4889(99)00151-2\"\n     onclick=\"log_download('shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000', 'http://doi.org/10.1016/S0167-4889(99)00151-2', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shcherbakova-filatov-camptothecinenhancesrandomintegrationoftransfecteddnaintothegenomeofmammaliancells-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shcherbakova20001,\r\nauthor={Shcherbakova, O.G. and Filatov, M.V.},\r\ntitle={Camptothecin enhances random integration of transfected DNA into the genome of mammalian cells},\r\njournal={Biochimica et Biophysica Acta - Molecular Cell Research},\r\nyear={2000},\r\nvolume={1495},\r\nnumber={1},\r\npages={1-3},\r\ndoi={10.1016/S0167-4889(99)00151-2},\r\nnote={cited By 10},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033982481&amp;doi=10.1016%2fS0167-4889%2899%2900151-2&amp;partnerID=40&amp;md5=da5b2e1f4286e2c7e96d04eac53c4b64},\r\naffiliation={Molec. and Radiat. Biophys. Division, Petersburg Nucl. Phys. Inst., 188350, Gatchina, Russian Federation},\r\nabstract={In order to study the involvement of DNA topoisomerase I (top1) in recombination, we examined the effect of the anti-neoplastic drug camptothecin, which selectively poisons top1 by trapping top1-cleavable complexes on integration of exogenic vector into the genome of mammalian cells. We transfected mouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a plasmid carrying a selectable neo gene treated with camptothecin, and determined the frequency of neo+ (G418(R)) colonies. We found that treatment with camptothecin for as short a time as 4 h after electroporation resulted in a 4- to 33-fold stimulation of plasmid integration into the recipient genome via non-homologous recombination. These results imply that top1-cleavable complexes trapped by camptothecin could be potentially recombinogenic structures and could stimulate non-homologous recombination in vivo, promoting the integration of transfected plasmids into mammalian genome. Copyright (C) 2000 Elsevier Science B.V.},\r\nauthor_keywords={Camptothecin;  Mammalian somatic cell;  Non-homologous recombination;  Topoisomerase 1;  Transfected DNA},\r\ncorrespondence_address1={Shcherbakova, O.G.; Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, 188350 Gatchina, Russian Federation; email: olia@omrb.pnpi.spb.ru},\r\nissn={01674889},\r\ncoden={BAMRD},\r\npubmed_id={10634926},\r\nlanguage={English},\r\nabbrev_source_title={Biochim. Biophys. Acta Mol. Cell Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  In order to study the involvement of DNA topoisomerase I (top1) in recombination, we examined the effect of the anti-neoplastic drug camptothecin, which selectively poisons top1 by trapping top1-cleavable complexes on integration of exogenic vector into the genome of mammalian cells. We transfected mouse F9 teratocarcinoma cells as well as Chinese hamster V79 cells with a plasmid carrying a selectable neo gene treated with camptothecin, and determined the frequency of neo+ (G418(R)) colonies. We found that treatment with camptothecin for as short a time as 4 h after electroporation resulted in a 4- to 33-fold stimulation of plasmid integration into the recipient genome via non-homologous recombination. These results imply that top1-cleavable complexes trapped by camptothecin could be potentially recombinogenic structures and could stimulate non-homologous recombination in vivo, promoting the integration of transfected plasmids into mammalian genome. Copyright (C) 2000 Elsevier Science B.V.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&amp;partnerID=40&amp;md5=6d2b5ab3cc3a34ddf31edb4d0a59e434\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&amp;partnerID=40&amp;md5=6d2b5ab3cc3a34ddf31edb4d0a59e434', event);\">\n    Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Scherbakova, O.; Lanzov, V.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry section / translated from Russian</i>, 371(1-6): 69-72.  2000.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&amp;partnerID=40&amp;md5=6d2b5ab3cc3a34ddf31edb4d0a59e434\"\n     onclick=\"log_download('scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&amp;partnerID=40&amp;md5=6d2b5ab3cc3a34ddf31edb4d0a59e434', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting. [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('scherbakova-lanzov-filatov-overexpressionofbacterialrecaproteininsomaticmammaliancellsincreasesthefrequencyofgenetargeting-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Scherbakova200069,\r\nauthor={Scherbakova, O.G. and Lanzov, V.A. and Filatov, M.V.},\r\ntitle={Overexpression of bacterial RecA protein in somatic mammalian cells increases the frequency of gene targeting.},\r\njournal={Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry section / translated from Russian},\r\nyear={2000},\r\nvolume={371},\r\nnumber={1-6},\r\npages={69-72},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034150613&amp;partnerID=40&amp;md5=6d2b5ab3cc3a34ddf31edb4d0a59e434},\r\naffiliation={Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\ncorrespondence_address1={Scherbakova, O.G.},\r\nissn={00124958},\r\npubmed_id={10853454},\r\nlanguage={English},\r\nabbrev_source_title={Dokl. Biochem.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&amp;doi=10.1007%2fBF02757259&amp;partnerID=40&amp;md5=741ca6fb3cfdb09a5156ff022f6829b1\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&amp;doi=10.1007%2fBF02757259&amp;partnerID=40&amp;md5=741ca6fb3cfdb09a5156ff022f6829b1', event);\">\n    A family of shuttle vectors for lactic acid bacteria and other gram-positive bacteria based on the plasmid pLF1311 replicon.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Microbiology</i>, 69(1): 63-67.  2000.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&amp;doi=10.1007%2fBF02757259&amp;partnerID=40&amp;md5=741ca6fb3cfdb09a5156ff022f6829b1\"\n     onclick=\"log_download('anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042438759&amp;doi=10.1007%2fBF02757259&amp;partnerID=40&amp;md5=741ca6fb3cfdb09a5156ff022f6829b1', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A family of shuttle vectors for lactic acid bacteria and other gram-positive bacteria based on the plasmid pLF1311 replicon [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/BF02757259\"\n     onclick=\"log_download('anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000', 'http://doi.org/10.1007/BF02757259', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-afamilyofshuttlevectorsforlacticacidbacteriaandothergrampositivebacteriabasedontheplasmidplf1311replicon-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. The vectors are based on the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM 1311, belonging to the family of the σ-type pE194-like plasmids. The vectors can replicate in gram-positive bacteria and Escherichia coli. They are stable in many gram-positive bacteria, have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPα can be mobilized in a great number of bacteria using a helper plasmid from E. coli but not from gram-positive bacteria. © 2000 MAIK \"Nauka/Interperiodica\".\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&amp;doi=10.1006%2fplas.1999.1455&amp;partnerID=40&amp;md5=86a151c76a9bd0d6c8f657503f14b257\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&amp;doi=10.1006%2fplas.1999.1455&amp;partnerID=40&amp;md5=86a151c76a9bd0d6c8f657503f14b257', event);\">\n    Expression of bacillar glutamyl endopeptidase genes in Bacillus subtilis by a new mobilizable single-replicon vector pLF.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Plasmid</i>, 43(3): 190-199.  2000.\n  <span class=\"note\">cited By 5</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&amp;doi=10.1006%2fplas.1999.1455&amp;partnerID=40&amp;md5=86a151c76a9bd0d6c8f657503f14b257\"\n     onclick=\"log_download('anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-18744436441&amp;doi=10.1006%2fplas.1999.1455&amp;partnerID=40&amp;md5=86a151c76a9bd0d6c8f657503f14b257', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Expression of bacillar glutamyl endopeptidase genes in Bacillus subtilis by a new mobilizable single-replicon vector pLF [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1006/plas.1999.1455\"\n     onclick=\"log_download('anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000', 'http://doi.org/10.1006/plas.1999.1455', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-expressionofbacillarglutamylendopeptidasegenesinbacillussubtilisbyanewmobilizablesinglerepliconvectorplf-2000')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The pLF1311 natural plasmid from Lactobacillus fermentum 1311 was used to construct a single replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and Escherichia coli. The new vector is capable of conjugative mobilization from E. coli to various hosts by conjugal transfer. The final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. Glutamyl endopeptidase genes from Bacillus licheniformis (gseBL) and B. intermedius (gseB1) were cloned in both pLF9 and pLF14 vectors and introduced to B. subtilis. The yield of enzymes in the pLF-derived producers was 6- to 30-fold more than in the natural producers and reached 100-150 mg/L of mature protease. (C) 2000 Academic Press.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1999\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1999')\"\n      onkeypress=\"toggleGroup('group_1999')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1999</span>\n      <span class=\"bibbase_group_count\">\n        \n        (9)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&amp;partnerID=40&amp;md5=b75439e5584ecc0bb4557fa9b5830fdf\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&amp;partnerID=40&amp;md5=b75439e5584ecc0bb4557fa9b5830fdf', event);\">\n    Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Varfolomeeva, E.; Levina, V.; Tret'yakov, A.; Drobchenko, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Doklady Akademii Nauk</i>, 364(3): 402-404.  1999.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&amp;partnerID=40&amp;md5=b75439e5584ecc0bb4557fa9b5830fdf\"\n     onclick=\"log_download('varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&amp;partnerID=40&amp;md5=b75439e5584ecc0bb4557fa9b5830fdf', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('varfolomeeva-levina-tretyakov-drobchenko-filatov-flowcytifluorometricinvestigationofactivedissociationofnotcovalentlybindagentsfromdnainhumancells-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Varfolomeeva1999402,\r\nauthor={Varfolomeeva, E.Yu. and Levina, V.V. and Tret&#x27;yakov, A.N. and Drobchenko, E.A. and Filatov, M.V.},\r\ntitle={Flow cytifluorometric investigation of active dissociation of not covalently bind agents from DNA in human cells},\r\njournal={Doklady Akademii Nauk},\r\nyear={1999},\r\nvolume={364},\r\nnumber={3},\r\npages={402-404},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032618178&amp;partnerID=40&amp;md5=b75439e5584ecc0bb4557fa9b5830fdf},\r\nissn={08695652},\r\ncoden={DAKNE},\r\npubmed_id={10188092},\r\nlanguage={English; Russian},\r\nabbrev_source_title={Dokl. Akad. Nauk},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&amp;partnerID=40&amp;md5=f0d90567c33a31e593c4e7a9fbbcaedf\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&amp;partnerID=40&amp;md5=f0d90567c33a31e593c4e7a9fbbcaedf', event);\">\n    Hot start of the polymerase chain reaction using DNA helicases.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kaboev, O.; Shevelev, I.; Luchkina, L.; Tret'yakov, A.;  and Shcherbakova, O.\n</span>\n\n  \n\n</span>\n\n  <i>Bioorganicheskaya Khimiya</i>, 25(5): 400.  1999.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&amp;partnerID=40&amp;md5=f0d90567c33a31e593c4e7a9fbbcaedf\"\n     onclick=\"log_download('kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&amp;partnerID=40&amp;md5=f0d90567c33a31e593c4e7a9fbbcaedf', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Hot start of the polymerase chain reaction using DNA helicases [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kaboev1999400,\r\nauthor={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret&#x27;yakov, A.N. and Shcherbakova, O.G.},\r\ntitle={Hot start of the polymerase chain reaction using DNA helicases},\r\njournal={Bioorganicheskaya Khimiya},\r\nyear={1999},\r\nvolume={25},\r\nnumber={5},\r\npages={400},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039697063&amp;partnerID=40&amp;md5=f0d90567c33a31e593c4e7a9fbbcaedf},\r\naffiliation={Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\r\nabstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.},\r\nauthor_keywords={DNA helicase;  Hot start;  PCR},\r\ncorrespondence_address1={Kaboev, O.K.; Petersburg Inst. for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.spb.ru},\r\nissn={01323423},\r\nlanguage={Russian},\r\nabbrev_source_title={Bioorganicheskaya Khim.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&amp;doi=10.1093%2fmolehr%2f5.9.825&amp;partnerID=40&amp;md5=749736b469111d208fd9a86f6f59e531\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&amp;doi=10.1093%2fmolehr%2f5.9.825&amp;partnerID=40&amp;md5=749736b469111d208fd9a86f6f59e531', event);\">\n    Relationship between abnormal sperm chromatin packing and IVF results.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Semenova, E.; Vorob'eva, O.; Leont'eva, O.;  and Drobchenko, E.\n</span>\n\n  \n\n</span>\n\n  <i>Molecular Human Reproduction</i>, 5(9): 825-830.  1999.\n  <span class=\"note\">cited By 62</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&amp;doi=10.1093%2fmolehr%2f5.9.825&amp;partnerID=40&amp;md5=749736b469111d208fd9a86f6f59e531\"\n     onclick=\"log_download('filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&amp;doi=10.1093%2fmolehr%2f5.9.825&amp;partnerID=40&amp;md5=749736b469111d208fd9a86f6f59e531', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Relationship between abnormal sperm chromatin packing and IVF results [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1093/molehr/5.9.825\"\n     onclick=\"log_download('filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999', 'http://doi.org/10.1093/molehr/5.9.825', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-semenova-vorobeva-leonteva-drobchenko-relationshipbetweenabnormalspermchromatinpackingandivfresults-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1999825,\r\nauthor={Filatov, M.V. and Semenova, E.V. and Vorob&#x27;eva, O.A. and Leont&#x27;eva, O.A. and Drobchenko, E.A.},\r\ntitle={Relationship between abnormal sperm chromatin packing and IVF results},\r\njournal={Molecular Human Reproduction},\r\nyear={1999},\r\nvolume={5},\r\nnumber={9},\r\npages={825-830},\r\ndoi={10.1093/molehr/5.9.825},\r\nnote={cited By 62},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032838550&amp;doi=10.1093%2fmolehr%2f5.9.825&amp;partnerID=40&amp;md5=749736b469111d208fd9a86f6f59e531},\r\naffiliation={Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},\r\nabstract={This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross- linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.},\r\nauthor_keywords={Chromatin packing;  Ethidium bromide;  Fertility disorder;  Flow cytometry;  Human spermatozoa},\r\ncorrespondence_address1={Filatov, M.V.; Dept. of Molec. Radiobiol./Biophys., St Petersburg Nuclear Physics Inst., Rassian AS, 188350 Gatchina, Russian Federation},\r\nissn={13609947},\r\ncoden={MHREF},\r\npubmed_id={10460220},\r\nlanguage={English},\r\nabbrev_source_title={Mol. Hum. Reprod.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross- linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&amp;doi=10.1016%2fS0378-1097%2899%2900343-2&amp;partnerID=40&amp;md5=dc537540b7e1b1d040043daddc06b0e6\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&amp;doi=10.1016%2fS0378-1097%2899%2900343-2&amp;partnerID=40&amp;md5=dc537540b7e1b1d040043daddc06b0e6', event);\">\n    The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>FEMS Microbiology Letters</i>, 178(1): 47-53.  1999.\n  <span class=\"note\">cited By 12</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&amp;doi=10.1016%2fS0378-1097%2899%2900343-2&amp;partnerID=40&amp;md5=dc537540b7e1b1d040043daddc06b0e6\"\n     onclick=\"log_download('anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032780658&amp;doi=10.1016%2fS0378-1097%2899%2900343-2&amp;partnerID=40&amp;md5=dc537540b7e1b1d040043daddc06b0e6', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/S0378-1097(99)00343-2\"\n     onclick=\"log_download('anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999', 'http://doi.org/10.1016/S0378-1097(99)00343-2', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-thebroadhostrangeplasmidplf1311fromlactobacillusfermentumvkm1311-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram-positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non-selective conditions. Copyright (C) 1999 Federation of European Microbiological Societies.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&amp;doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&amp;partnerID=40&amp;md5=3f6b63ef15d5a83f4e04cddb3b81eaeb\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&amp;doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&amp;partnerID=40&amp;md5=3f6b63ef15d5a83f4e04cddb3b81eaeb', event);\">\n    Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Naryzhny, S.; Levina, V.; Varfolomeeva, E.; Drobchenko, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Electrophoresis</i>, 20(4-5): 1033-1038.  1999.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&amp;doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&amp;partnerID=40&amp;md5=3f6b63ef15d5a83f4e04cddb3b81eaeb\"\n     onclick=\"log_download('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&amp;doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&amp;partnerID=40&amp;md5=3f6b63ef15d5a83f4e04cddb3b81eaeb', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it? [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5&lt;1033::AID-ELPS1033&gt;3.0.CO;2-3\"\n     onclick=\"log_download('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999', 'http://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5&lt;1033::AID-ELPS1033&gt;3.0.CO;2-3', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('naryzhny-levina-varfolomeeva-drobchenko-filatov-activedissociationofthefluorescentdyehoechst33342fromdnainalivingcellwhocoulddoit-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Naryzhny19991033,\r\nauthor={Naryzhny, S.N. and Levina, V.V. and Varfolomeeva, E.Y. and Drobchenko, E.A. and Filatov, M.V.},\r\ntitle={Active dissociation of the fluorescent dye Hoechst 33342 from DNA in a living cell: Who could do it?},\r\njournal={Electrophoresis},\r\nyear={1999},\r\nvolume={20},\r\nnumber={4-5},\r\npages={1033-1038},\r\ndoi={10.1002/(SICI)1522-2683(19990101)20:4/5&lt;1033::AID-ELPS1033&gt;3.0.CO;2-3},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032957473&amp;doi=10.1002%2f%28SICI%291522-2683%2819990101%2920%3a4%2f5%3c1033%3a%3aAID-ELPS1033%3e3.0.CO%3b2-3&amp;partnerID=40&amp;md5=3f6b63ef15d5a83f4e04cddb3b81eaeb},\r\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, Russian Federation; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad district, 188350, Russian Federation},\r\nabstract={It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA.},\r\nauthor_keywords={DNA clearing;  Hoechst 33342;  Mammalian cells;  Two-dimensional polyacrylamide gel electrophoresis},\r\ncorrespondence_address1={Naryzhny, S.N.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad District 188350, Russian Federation; email: naryzhny@omrb.pnpi.spb.ru},\r\nissn={01730835},\r\ncoden={ELCTD},\r\npubmed_id={10344282},\r\nlanguage={English},\r\nabbrev_source_title={Electrophoresis},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  It is assumed that DNA in mammalian cells is a dynamic conformationally unstable system. This instability provides the cell with a mechanism for dissociating a large number of substances that bind tightly but not covalently to DNA. Among these is the fluorescent dye Hoechst 33342, which binds to DNA in the minor groove. We have selected cell lines with a high capability for active dissociation of Hoechst 33342. Comparative protein analysis of these lines by means of two-dimensional (2-D) electrophoresis was performed. Cell and nuclear proteins were analyzed from these and normal strains. A few proteins with significantly changed quantities have been found. The preliminary search of the 2-D database allowed us to identity some known and unknown cellular proteins that could participate in active dissociation of the dye from DNA.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&amp;partnerID=40&amp;md5=72d82556ef27e324b46c8a4c6b27a4b9\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&amp;partnerID=40&amp;md5=72d82556ef27e324b46c8a4c6b27a4b9', event);\">\n    Hot start of the polymerase chain reaction using DNA helicases.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Kaboev, O.; Shevelev, I.; Luchkina, L.; Tret'yakov, A.;  and Shcherbakova, O.\n</span>\n\n  \n\n</span>\n\n  <i>Russian Journal of Bioorganic Chemistry</i>, 25(5): 350-351.  1999.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&amp;partnerID=40&amp;md5=72d82556ef27e324b46c8a4c6b27a4b9\"\n     onclick=\"log_download('kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&amp;partnerID=40&amp;md5=72d82556ef27e324b46c8a4c6b27a4b9', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Hot start of the polymerase chain reaction using DNA helicases [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kaboev-shevelev-luchkina-tretyakov-shcherbakova-hotstartofthepolymerasechainreactionusingdnahelicases-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Kaboev1999350,\r\nauthor={Kaboev, O.K. and Shevelev, I.V. and Luchkina, L.A. and Tret&#x27;yakov, A.N. and Shcherbakova, O.G.},\r\ntitle={Hot start of the polymerase chain reaction using DNA helicases},\r\njournal={Russian Journal of Bioorganic Chemistry},\r\nyear={1999},\r\nvolume={25},\r\nnumber={5},\r\npages={350-351},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644556225&amp;partnerID=40&amp;md5=72d82556ef27e324b46c8a4c6b27a4b9},\r\naffiliation={St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\r\nabstract={A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture. © 1999 MAEe cyrillic signK &quot;Hayκa/Interperiodica&quot;.},\r\nauthor_keywords={DNA helicase;  Hot start;  PCR},\r\ncorrespondence_address1={Kaboev, O.K.; St. Petersburg Institute for Nuclear Physics, Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: kaboev@omrb.pnpi.sbr.ru},\r\nissn={10681620},\r\nlanguage={English},\r\nabbrev_source_title={Russ. J. Bioorg. Chem.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A novel method for the hot start of PCR using DNA helicases is developed. The addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mixture and initial heating. The hot start of PCR occurs automatically after inactivation of the DNA helicase upon heating of the reaction mixture. © 1999 MAEe cyrillic signK \"Hayκa/Interperiodica\".\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&amp;partnerID=40&amp;md5=ff82ecf873c4549e7c93f8fae7ce5775\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&amp;partnerID=40&amp;md5=ff82ecf873c4549e7c93f8fae7ce5775', event);\">\n    Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Shcherbakova, O.; Smirnova, T.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 41(11): 951.  1999.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&amp;partnerID=40&amp;md5=ff82ecf873c4549e7c93f8fae7ce5775\"\n     onclick=\"log_download('shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&amp;partnerID=40&amp;md5=ff82ecf873c4549e7c93f8fae7ce5775', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shcherbakova-smirnova-filatov-effectsofinhibitorsofpolyadpribozylationandtopoisomerasesonthefrequencyofhomologousandrandomintegrationoftransfecteddnaintogenomeofmammaliansomaticcells-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Shcherbakova1999951,\r\nauthor={Shcherbakova, O.G. and Smirnova, T.V. and Filatov, M.V.},\r\ntitle={Effects of inhibitors of poly(ADP-ribozylation) and topoisomerases on the frequency of homologous and random integration of transfected DNA into genome of mammalian somatic cells},\r\njournal={Tsitologiya},\r\nyear={1999},\r\nvolume={41},\r\nnumber={11},\r\npages={951},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0043265030&amp;partnerID=40&amp;md5=ff82ecf873c4549e7c93f8fae7ce5775},\r\nabstract={A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system.},\r\nauthor_keywords={Gene targeting;  hprt;  Illegitimate recombination;  Poly(ADP-ribose) polymerase;  Topoisomerases},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={10643051},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ivanov-vitamineblockingthebeginningsofatherosclerosis-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&amp;doi=10.1016%2fS1357-4310%2898%2901402-6&amp;partnerID=40&amp;md5=038c62d4e6a2ed7c1c6f416237e01f51\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ivanov-vitamineblockingthebeginningsofatherosclerosis-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&amp;doi=10.1016%2fS1357-4310%2898%2901402-6&amp;partnerID=40&amp;md5=038c62d4e6a2ed7c1c6f416237e01f51', event);\">\n    Vitamin E: blocking the beginnings of atherosclerosis?.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ivanov, E.\n</span>\n\n  \n\n</span>\n\n  <i>Molecular medicine today</i>, 5(1): 8.  1999.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&amp;doi=10.1016%2fS1357-4310%2898%2901402-6&amp;partnerID=40&amp;md5=038c62d4e6a2ed7c1c6f416237e01f51\"\n     onclick=\"log_download('ivanov-vitamineblockingthebeginningsofatherosclerosis-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&amp;doi=10.1016%2fS1357-4310%2898%2901402-6&amp;partnerID=40&amp;md5=038c62d4e6a2ed7c1c6f416237e01f51', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Vitamin E: blocking the beginnings of atherosclerosis? [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/S1357-4310(98)01402-6\"\n     onclick=\"log_download('ivanov-vitamineblockingthebeginningsofatherosclerosis-1999', 'http://doi.org/10.1016/S1357-4310(98)01402-6', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ivanov-vitamineblockingthebeginningsofatherosclerosis-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ivanov-vitamineblockingthebeginningsofatherosclerosis-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ivanov19998,\r\nauthor={Ivanov, E.},\r\ntitle={Vitamin E: blocking the beginnings of atherosclerosis?},\r\njournal={Molecular medicine today},\r\nyear={1999},\r\nvolume={5},\r\nnumber={1},\r\npages={8},\r\ndoi={10.1016/S1357-4310(98)01402-6},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032603844&amp;doi=10.1016%2fS1357-4310%2898%2901402-6&amp;partnerID=40&amp;md5=038c62d4e6a2ed7c1c6f416237e01f51},\r\ncorrespondence_address1={Ivanov, E.email: eivanov@aol.com},\r\nissn={13574310},\r\npubmed_id={10088123},\r\nlanguage={English},\r\nabbrev_source_title={Mol Med Today},\r\ndocument_type={Short Survey},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&amp;doi=10.1089%2fhyb.1999.18.99&amp;partnerID=40&amp;md5=2b2d61bdf666822de71b5967cb78bb5c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&amp;doi=10.1089%2fhyb.1999.18.99&amp;partnerID=40&amp;md5=2b2d61bdf666822de71b5967cb78bb5c', event);\">\n    Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Tarasov, V.; Filatov, M.; Kisliakova, T.; Noskov, F.; Koloskov, A.; Stavrovietski, V.; Onikienko, S.; Kletchikov, V.; Lvov, I.; Varfolomeeva, E.; Blizniukov, O.; Levina, V.;  and Kiselevski, M.\n</span>\n\n  \n\n</span>\n\n  <i>Hybridoma</i>, 18(1): 99-102.  1999.\n  <span class=\"note\">cited By 5</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&amp;doi=10.1089%2fhyb.1999.18.99&amp;partnerID=40&amp;md5=2b2d61bdf666822de71b5967cb78bb5c\"\n     onclick=\"log_download('tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&amp;doi=10.1089%2fhyb.1999.18.99&amp;partnerID=40&amp;md5=2b2d61bdf666822de71b5967cb78bb5c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1089/hyb.1999.18.99\"\n     onclick=\"log_download('tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999', 'http://doi.org/10.1089/hyb.1999.18.99', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('tarasov-filatov-kisliakova-noskov-koloskov-stavrovietski-onikienko-kletchikov-etal-combinedsurgicalandimmunotherapeutictreatmentofpatientswithfourthstagecoloncancer-1999')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Tarasov199999,\r\nauthor={Tarasov, V.A. and Filatov, M.V. and Kisliakova, T.V. and Noskov, F.S. and Koloskov, A.V. and Stavrovietski, V.V. and Onikienko, S.B. and Kletchikov, V.Z. and Lvov, I.V. and Varfolomeeva, E.Yu. and Blizniukov, O.P. and Levina, V.V. and Kiselevski, M.V.},\r\ntitle={Combined surgical and immunotherapeutic treatment of patients with fourth stage colon cancer},\r\njournal={Hybridoma},\r\nyear={1999},\r\nvolume={18},\r\nnumber={1},\r\npages={99-102},\r\ndoi={10.1089/hyb.1999.18.99},\r\nnote={cited By 5},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033035804&amp;doi=10.1089%2fhyb.1999.18.99&amp;partnerID=40&amp;md5=2b2d61bdf666822de71b5967cb78bb5c},\r\naffiliation={Department of Thorax Surgery, Med. Acad. of Postgraduated Educ., 196247, St. Petersburg, Russian Federation; City Hospital No. 26, 196247, St. Petersburg, Russian Federation; Petersburg Nuclear Physics Institute, St. Petersburg, 188350, Russian Federation; Petersburg Nuclear Physics Institute, 188350, Gatchina, St. Petersburg, Russian Federation},\r\nabstract={Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilie de Calmette-Guerin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.},\r\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, 188350 Gatchina, St. Petersburg, Russian Federation},\r\npublisher={Mary Ann Liebert Inc.},\r\nissn={0272457X},\r\ncoden={HYBRD},\r\npubmed_id={10211796},\r\nlanguage={English},\r\nabbrev_source_title={Hybridoma},\r\ndocument_type={Conference Paper},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Sixty-five patients with the fourth stage colon cancer were subjected to the combined surgical and immunotherapy. The following conclusions are made: (1) surgical elimination of the bulk of tumor mass is a necessary prerequisite for effective immunotherapy; (2) vaccination with autological tumor cells accompanied with bacille bilie de Calmette-Guerin (BCG) as the adjuvant and with interleukin-2 as the immunostimulator effectively prevents metastasizing after successful surgery; (3) the vaccine must necessary contain living tumor cells adequately presenting tumor antigens; and (4) in some cases, immunotherapy causes undesirable autoimmune complications. They can be registered by corresponding inflammation control methods.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1998\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1998')\"\n      onkeypress=\"toggleGroup('group_1998')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1998</span>\n      <span class=\"bibbase_group_count\">\n        \n        (8)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&amp;partnerID=40&amp;md5=a98a72c5e0367996d29f6a7ca63e9233\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&amp;partnerID=40&amp;md5=a98a72c5e0367996d29f6a7ca63e9233', event);\">\n    \"DNA clearing\" from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Levina, V.; Varfolomeeva, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Biologicheskie Membrany</i>, 15(6): 706-707.  1998.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&amp;partnerID=40&amp;md5=a98a72c5e0367996d29f6a7ca63e9233\"\n     onclick=\"log_download('levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&amp;partnerID=40&amp;md5=a98a72c5e0367996d29f6a7ca63e9233', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"&quot;DNA clearing&quot; from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Levina1998706,\r\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},\r\ntitle={&quot;DNA clearing&quot; from noncovalently bound agents in mammalian cells as a new mechanism of drug resistance},\r\njournal={Biologicheskie Membrany},\r\nyear={1998},\r\nvolume={15},\r\nnumber={6},\r\npages={706-707},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0039392095&amp;partnerID=40&amp;md5=a98a72c5e0367996d29f6a7ca63e9233},\r\naffiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\ncorrespondence_address1={Levina, V.V.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\nissn={02334755},\r\nlanguage={Russian},\r\nabbrev_source_title={Biol. Membr.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&amp;partnerID=40&amp;md5=693d0527f04b69d04f82f18f75215244\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&amp;partnerID=40&amp;md5=693d0527f04b69d04f82f18f75215244', event);\">\n    'DNA clearing' from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Levina, V.; Varfolomeeva, E.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Membrane and Cell Biology</i>, 12(6): 883-893.  1998.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&amp;partnerID=40&amp;md5=693d0527f04b69d04f82f18f75215244\"\n     onclick=\"log_download('levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&amp;partnerID=40&amp;md5=693d0527f04b69d04f82f18f75215244', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"&#x27;DNA clearing&#x27; from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('levina-varfolomeeva-filatov-dnaclearingfromnoncovalentlyboundagentsinmammaliancellsasanewmechanismofdrugresistance-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Levina1998883,\r\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Filatov, M.V.},\r\ntitle={&#x27;DNA clearing&#x27; from non-covalently bound agents in mammalian cells as a new mechanism of drug resistance},\r\njournal={Membrane and Cell Biology},\r\nyear={1998},\r\nvolume={12},\r\nnumber={6},\r\npages={883-893},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032324415&amp;partnerID=40&amp;md5=693d0527f04b69d04f82f18f75215244},\r\naffiliation={St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation},\r\nabstract={Earlier, we have described the process of active dissociation or &#x27;DNA clearing&#x27; from non-covalently bound agents in living mammalian cells. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds DNA in the minor groove tightly but non-covalently, was used for studying the interaction of non-covalently binding agents with DNA. Multiple drug resistance (MDR) in tumour cells is related to the expression of transport proteins that alter the cellular drug transport and distribution. Three different groups of genes (mdr, MRP, and LRP) and their products are implicated in MDR. To obtain new cell lines characterized by enhanced process of active dissociation of non-covalently bound agents from DNA or &#x27;DNA clearing&#x27;, we carried out step-by-step selection with increasing concentrations of Hoechst 33342. The rodent cell lines hyperresistant to Hoechst 33342 and selected from AA8 were named AA8Hoe-R-1-AA8Hoe-R-10, and the cell lines selected from L cells were called LHoe-R-1-LHoe-R-10. The most resistant of them, AA8Hoe-R-6 and AA8Hoe-R-7, were able to grow in the presence of 80 μm/ml of Hoechst 33342 in the cell culture medium. All mutants were analyzed with the flow cytometric technique and were divided into two different groups. We conclude that the drug resistance of the first group of cell lines was due to changes in transport proteins. The second group of the resistant cell lines was characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. As we believe, the enhanced level of &#x27;DNA clearing&#x27; was caused by the amplification of some genes, because the gradual increase of Hoechst resistance in the same cell line resulted from the increase in the ability to remove the dye from DNA. These lines were shown to be also resistant to netropsin.},\r\ncorrespondence_address1={Levina, V.V.; St. Petersburg Inst. Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region 188350, Russian Federation; email: levina@ombr.pnpi.spb.ru},\r\nissn={10236597},\r\ncoden={MCBIE},\r\npubmed_id={10512056},\r\nlanguage={English},\r\nabbrev_source_title={Membr. Cell Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Earlier, we have described the process of active dissociation or 'DNA clearing' from non-covalently bound agents in living mammalian cells. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds DNA in the minor groove tightly but non-covalently, was used for studying the interaction of non-covalently binding agents with DNA. Multiple drug resistance (MDR) in tumour cells is related to the expression of transport proteins that alter the cellular drug transport and distribution. Three different groups of genes (mdr, MRP, and LRP) and their products are implicated in MDR. To obtain new cell lines characterized by enhanced process of active dissociation of non-covalently bound agents from DNA or 'DNA clearing', we carried out step-by-step selection with increasing concentrations of Hoechst 33342. The rodent cell lines hyperresistant to Hoechst 33342 and selected from AA8 were named AA8Hoe-R-1-AA8Hoe-R-10, and the cell lines selected from L cells were called LHoe-R-1-LHoe-R-10. The most resistant of them, AA8Hoe-R-6 and AA8Hoe-R-7, were able to grow in the presence of 80 μm/ml of Hoechst 33342 in the cell culture medium. All mutants were analyzed with the flow cytometric technique and were divided into two different groups. We conclude that the drug resistance of the first group of cell lines was due to changes in transport proteins. The second group of the resistant cell lines was characterized by an enhanced dissociation of the bisbenzimidazole dye-DNA complex. As we believe, the enhanced level of 'DNA clearing' was caused by the amplification of some genes, because the gradual increase of Hoechst resistance in the same cell line resulted from the increase in the ability to remove the dye from DNA. These lines were shown to be also resistant to netropsin.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&amp;partnerID=40&amp;md5=15f307298c0ecbf7063bce7528f1f30b\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&amp;partnerID=40&amp;md5=15f307298c0ecbf7063bce7528f1f30b', event);\">\n    Influence of polyelectrolyte properties of DNA on cytosine protonation.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ivanovo, M.; Smolin, A.; Shcherbakova, O.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Molekulyarnaya Biologiya</i>, 32(4): 635-638.  1998.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&amp;partnerID=40&amp;md5=15f307298c0ecbf7063bce7528f1f30b\"\n     onclick=\"log_download('ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&amp;partnerID=40&amp;md5=15f307298c0ecbf7063bce7528f1f30b', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Influence of polyelectrolyte properties of DNA on cytosine protonation [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ivanovo-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ivanovo1998635,\r\nauthor={Ivanovo, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},\r\ntitle={Influence of polyelectrolyte properties of DNA on cytosine protonation},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={1998},\r\nvolume={32},\r\nnumber={4},\r\npages={635-638},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032112024&amp;partnerID=40&amp;md5=15f307298c0ecbf7063bce7528f1f30b},\r\nissn={00268984},\r\ncoden={MOBIB},\r\npubmed_id={9785567},\r\nlanguage={Russian},\r\nabbrev_source_title={Mol. Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&amp;partnerID=40&amp;md5=492e8c62636367903a8e064e4bf17882\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&amp;partnerID=40&amp;md5=492e8c62636367903a8e064e4bf17882', event);\">\n    Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Microbiology</i>, 67(1): 23-27.  1998.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&amp;partnerID=40&amp;md5=492e8c62636367903a8e064e4bf17882\"\n     onclick=\"log_download('safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&amp;partnerID=40&amp;md5=492e8c62636367903a8e064e4bf17882', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova199823,\r\nauthor={Safronova, I.Yu. and Semenova, E.V.},\r\ntitle={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},\r\njournal={Microbiology},\r\nyear={1998},\r\nvolume={67},\r\nnumber={1},\r\npages={23-27},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644450173&amp;partnerID=40&amp;md5=492e8c62636367903a8e064e4bf17882},\r\naffiliation={Moscow State University, Vorob&#x27;eve gory, Moscow, 119899, Russian Federation},\r\nabstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cistrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in the growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6. © 1998 MAHK Hayka/lnterperiodica Publishing.},\r\nauthor_keywords={Alcaligenes xylosoxidans subsp. xylosoxidans;  L-malate;  Maleate cis-trans-isomerase;  Maleic acid;  Transformation},\r\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Vorob&#x27;eve gory, Moscow, 119899, Russian Federation},\r\nissn={00262617},\r\nlanguage={English},\r\nabbrev_source_title={Microbiology},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cistrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in the growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6. © 1998 MAHK Hayka/lnterperiodica Publishing.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&amp;partnerID=40&amp;md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&amp;partnerID=40&amp;md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c', event);\">\n    Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Safronova, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Mikrobiologiya</i>, 67(1): 30-34.  1998.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&amp;partnerID=40&amp;md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c\"\n     onclick=\"log_download('safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&amp;partnerID=40&amp;md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260 [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('safronova-semenova-maleatecistransisomeraseactivityofalcaligenesxylosoxidanssubspxylosoxidans260-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Safronova199830,\r\nauthor={Safronova, I.Yu. and Semenova, E.V.},\r\ntitle={Maleate cis-trans-isomerase activity of alcaligenes xylosoxidans subsp. xylosoxidans 260},\r\njournal={Mikrobiologiya},\r\nyear={1998},\r\nvolume={67},\r\nnumber={1},\r\npages={30-34},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141425939&amp;partnerID=40&amp;md5=aa1f0509a7e4a200d1ff0d3dea1c7f5c},\r\naffiliation={Moscow State University, Vorob&#x27;eve gory, Moscow, 119899, Russian Federation},\r\nabstract={Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cisfrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6.},\r\nauthor_keywords={Alcaligenes xylosoxidans subsp;  L-malate;  Maleate cis-transisomerase;  Maleic acid;  Transformation;  Xylosoxidans},\r\ncorrespondence_address1={Safronova, I.Yu.; Moscow State University, Vorob&#x27;eve gory, Moscow, 119899, Russian Federation},\r\nissn={00263656},\r\ncoden={MIKBA},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikrobiologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Alcaligenes xylosoxidans subsp. xylosoxidans 260 KM MGU was able to utilize maleic acid with the accumulation of L-malic, acetic, and propionic acids in the culture liquid. Maleic acid was found to enter the tricarboxylic acid cycle at the level of fumaric acid under the action of the membrane-bound maleate cisfrans-isomerase (EC 5.2.1.1). A. xylosoxidans was able to transform maleic acid throughout the entire cultivation period; however, the specific rate of maleic acid consumption peaked by the onset of the stationary phase, since the transformation of maleic acid was inhibited as a result of the accumulation and utilization of its transformation product, i.e., malate. The presence of intermediates of the tricarboxylic acid cycle, such as α-ketoglutarate or citrate, in growth medium as the second carbon sources delayed the transformation of maleic acid, probably, due to competitive inhibition. Maleic acid completely inhibited catabolism of sugars (glucose, xylose, fructose). The maleate isomerase activity of intact cells, toluene-permeabilized cells, and cell-free extract peaked at +40°C and pH 7.6.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&amp;doi=10.1016%2fS0027-5107%2898%2900056-6&amp;partnerID=40&amp;md5=63c03315650103ce0876961fb7f3630c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&amp;doi=10.1016%2fS0027-5107%2898%2900056-6&amp;partnerID=40&amp;md5=63c03315650103ce0876961fb7f3630c', event);\">\n    Methods for registration of spontaneous DNA instability in mammalian cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Pantina, R.;  and Noskin, L.\n</span>\n\n  \n\n</span>\n\n  <i>Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis</i>, 403(1-2): 95-101.  1998.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&amp;doi=10.1016%2fS0027-5107%2898%2900056-6&amp;partnerID=40&amp;md5=63c03315650103ce0876961fb7f3630c\"\n     onclick=\"log_download('filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&amp;doi=10.1016%2fS0027-5107%2898%2900056-6&amp;partnerID=40&amp;md5=63c03315650103ce0876961fb7f3630c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Methods for registration of spontaneous DNA instability in mammalian cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/S0027-5107(98)00056-6\"\n     onclick=\"log_download('filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998', 'http://doi.org/10.1016/S0027-5107(98)00056-6', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-pantina-noskin-methodsforregistrationofspontaneousdnainstabilityinmammaliancells-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov199895,\r\nauthor={Filatov, M.V. and Pantina, R.A. and Noskin, L.A.},\r\ntitle={Methods for registration of spontaneous DNA instability in mammalian cells},\r\njournal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},\r\nyear={1998},\r\nvolume={403},\r\nnumber={1-2},\r\npages={95-101},\r\ndoi={10.1016/S0027-5107(98)00056-6},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032540955&amp;doi=10.1016%2fS0027-5107%2898%2900056-6&amp;partnerID=40&amp;md5=63c03315650103ce0876961fb7f3630c},\r\naffiliation={St. Petersburg Nucl. Phys. Institute, Gatchina, St. Petersburg 188350, Russian Federation},\r\nabstract={A phenomenon of spontaneous DNA instability displays itself as the low level of repair DNA synthesis that takes place during any cell cycle phases. However, there is a problem in detection of very low intensive repair DNA synthesis. This paper suggests two approaches to detect the spontaneous DNA instability. The first method involves a blockade of the DNA gaps sealing by a combination of inhibitors, hydroxyurea and arabinofuranosyl cytosine. An accumulation of single strand gaps leads to production of DNA double strand breaks and results to reproductive inactivation of cells. It was shown that registration of both these events by different methods (such as viscoelastometry of DNA, orthogonal pulse electrophoresis or comet assay for double strand breaks as well as effectiveness of colony growth for cell inactivation) may be used as suitable measure of the spontaneous DNA instability. The second approach bases on photolysis of bromodeoxyuridine incorporated into repair DNA patches during the spontaneous repair DNA synthesis. Long wave UV irradiation of cells containing bromodeoxyuridine labeled DNA stained with Hoechst 33342 causes their inactivation. Experimental results presented confirm that both methods actually detect the spontaneous DNA instability. It takes note of the spontaneous DNA instability varies for cells from different tissues and species and increases during aging.},\r\nauthor_keywords={DNA synthesis inhibition;  Repair DNA synthesis;  Spontaneous DNA instability},\r\ncorrespondence_address1={Filatov, M.V.; St. Petersburg Nuclear Physics Inst., Gatchina, St. Petersburg 188350, Russian Federation; email: filatov@omrb.pnpi.spb.ru},\r\nissn={00275107},\r\ncoden={MRFME},\r\npubmed_id={9726010},\r\nlanguage={English},\r\nabbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A phenomenon of spontaneous DNA instability displays itself as the low level of repair DNA synthesis that takes place during any cell cycle phases. However, there is a problem in detection of very low intensive repair DNA synthesis. This paper suggests two approaches to detect the spontaneous DNA instability. The first method involves a blockade of the DNA gaps sealing by a combination of inhibitors, hydroxyurea and arabinofuranosyl cytosine. An accumulation of single strand gaps leads to production of DNA double strand breaks and results to reproductive inactivation of cells. It was shown that registration of both these events by different methods (such as viscoelastometry of DNA, orthogonal pulse electrophoresis or comet assay for double strand breaks as well as effectiveness of colony growth for cell inactivation) may be used as suitable measure of the spontaneous DNA instability. The second approach bases on photolysis of bromodeoxyuridine incorporated into repair DNA patches during the spontaneous repair DNA synthesis. Long wave UV irradiation of cells containing bromodeoxyuridine labeled DNA stained with Hoechst 33342 causes their inactivation. Experimental results presented confirm that both methods actually detect the spontaneous DNA instability. It takes note of the spontaneous DNA instability varies for cells from different tissues and species and increases during aging.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&amp;partnerID=40&amp;md5=913fa635ede6f8418b0b492cae1970a4\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&amp;partnerID=40&amp;md5=913fa635ede6f8418b0b492cae1970a4', event);\">\n    Influence of polyelectrolyte properties of DNA on cytosine protonation.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Ivanova, M.; Smolin, A.; Shcherbakova, O.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Molecular Biology</i>, 32(4): 523-526.  1998.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&amp;partnerID=40&amp;md5=913fa635ede6f8418b0b492cae1970a4\"\n     onclick=\"log_download('ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&amp;partnerID=40&amp;md5=913fa635ede6f8418b0b492cae1970a4', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Influence of polyelectrolyte properties of DNA on cytosine protonation [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('ivanova-smolin-shcherbakova-filatov-influenceofpolyelectrolytepropertiesofdnaoncytosineprotonation-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Ivanova1998523,\r\nauthor={Ivanova, M.A. and Smolin, A.V. and Shcherbakova, O.G. and Filatov, M.V.},\r\ntitle={Influence of polyelectrolyte properties of DNA on cytosine protonation},\r\njournal={Molecular Biology},\r\nyear={1998},\r\nvolume={32},\r\nnumber={4},\r\npages={523-526},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032351639&amp;partnerID=40&amp;md5=913fa635ede6f8418b0b492cae1970a4},\r\naffiliation={St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation},\r\nabstract={The polyelectrolyte properties of DNA affect cytosine protonation. The existence of a local region of lower pH in close proximity to the DNA chain is supposed to explain this phenomenon. Such a region was revealed by absorption spectroscopy with the use of Methyl Red (C15H15N3O2). Differences in the protonation of cytosines in different polyelectrolyte environments were detected spectrophotometrically under identical ionic and other conditions. The polyelectrolyte properties of DNA were proved to determine cytosine protonation at physiological pH. The differences in the protonation of cytosines were also observed in the studies of double-stranded high-and low-molecular DNA of the same nucleotide composition at low ionic strengths. The protonation of cytosine is shifted to the lower pH region for the high-molecular DNA as compared with fragments of lower molecular weight.},\r\nauthor_keywords={Absorption spectroscopy;  Cytosine protonation;  DNA;  Local pH;  Oligonucleotides;  Polyelectrolyte properties},\r\ncorrespondence_address1={Ivanova, M.A.; St. Petersburg Inst. of Nucl. Phys., Russian Academy of Sciences, Gatchina, 188350, Russian Federation; email: maria@omrb.pnpi.spb.ru},\r\nissn={00268933},\r\nlanguage={English},\r\nabbrev_source_title={Mol. Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The polyelectrolyte properties of DNA affect cytosine protonation. The existence of a local region of lower pH in close proximity to the DNA chain is supposed to explain this phenomenon. Such a region was revealed by absorption spectroscopy with the use of Methyl Red (C15H15N3O2). Differences in the protonation of cytosines in different polyelectrolyte environments were detected spectrophotometrically under identical ionic and other conditions. The polyelectrolyte properties of DNA were proved to determine cytosine protonation at physiological pH. The differences in the protonation of cytosines were also observed in the studies of double-stranded high-and low-molecular DNA of the same nucleotide composition at low ionic strengths. The protonation of cytosine is shifted to the lower pH region for the high-molecular DNA as compared with fragments of lower molecular weight.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&amp;partnerID=40&amp;md5=b71f1bc0675e0c35e7470ea90070a032\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&amp;partnerID=40&amp;md5=b71f1bc0675e0c35e7470ea90070a032', event);\">\n    Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Levina, V.; Varfolomeeva, E.; Drobchenko, E.; Tretyakov, A.; Klopov, N.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 40(10): 898-899.  1998.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&amp;partnerID=40&amp;md5=b71f1bc0675e0c35e7470ea90070a032\"\n     onclick=\"log_download('levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&amp;partnerID=40&amp;md5=b71f1bc0675e0c35e7470ea90070a032', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('levina-varfolomeeva-drobchenko-tretyakov-klopov-filatov-processofactivedissociationofvitaldyehoechst33342fromdnaincellsofculturedrodentcellularlines-1998')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Levina1998898,\r\nauthor={Levina, V.V. and Varfolomeeva, E.Yu. and Drobchenko, E.A. and Tretyakov, A.N. and Klopov, N.V. and Filatov, M.V.},\r\ntitle={Process of active dissociation of vital dye Hoechst 33342 from DNA in cells of cultured rodent cellular lines},\r\njournal={Tsitologiya},\r\nyear={1998},\r\nvolume={40},\r\nnumber={10},\r\npages={898-899},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542531555&amp;partnerID=40&amp;md5=b71f1bc0675e0c35e7470ea90070a032},\r\naffiliation={Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\nabstract={The process of active dissociation or «DNA clearing» of not covalently binding agents from DNA in living HeLa cells was shown by the flow cytometry technique. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds tightly but not covalently to DNA in a minor groove, was used as a basic model to study the interaction of not covalently binding agents with DNA. In this paper, we continue to analyse the «DNA clearing» process in the living fibroblasts of different rodent species (mouse, rat, Chinese hamster). The obtained data suggest that the processes of active dissociation or «DNA clearing» of Hoechst 33342 have some common features in all investigated mammalian cells. Nevertheless, some differences in the process were found in these lines. The role of nucleotide excision repair genes in the process of DNA clearing was not established.},\r\ncorrespondence_address1={Levina, V.V.; Nuclear Physics Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={9864821},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiya},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The process of active dissociation or «DNA clearing» of not covalently binding agents from DNA in living HeLa cells was shown by the flow cytometry technique. The vital fluorescent bisbenzimidazole dye Hoechst 33342, which binds tightly but not covalently to DNA in a minor groove, was used as a basic model to study the interaction of not covalently binding agents with DNA. In this paper, we continue to analyse the «DNA clearing» process in the living fibroblasts of different rodent species (mouse, rat, Chinese hamster). The obtained data suggest that the processes of active dissociation or «DNA clearing» of Hoechst 33342 have some common features in all investigated mammalian cells. Nevertheless, some differences in the process were found in these lines. The role of nucleotide excision repair genes in the process of DNA clearing was not established.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1997\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1997')\"\n      onkeypress=\"toggleGroup('group_1997')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1997</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&amp;partnerID=40&amp;md5=f26acee6ab5c8d940ffeac0eb2340e22\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&amp;partnerID=40&amp;md5=f26acee6ab5c8d940ffeac0eb2340e22', event);\">\n    Fast DNA Amplification in Tiny Ultrathin Microplates.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Tretyakov, A.; Pantina, R.;  and Kaboev, O.\n</span>\n\n  \n\n</span>\n\n  <i>Bioorganicheskaya Khimiya</i>, 23(6): 527-528.  1997.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&amp;partnerID=40&amp;md5=f26acee6ab5c8d940ffeac0eb2340e22\"\n     onclick=\"log_download('tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&amp;partnerID=40&amp;md5=f26acee6ab5c8d940ffeac0eb2340e22', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Fast DNA Amplification in Tiny Ultrathin Microplates [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('tretyakov-pantina-kaboev-fastdnaamplificationintinyultrathinmicroplates-1997')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Tretyakov1997527,\r\nauthor={Tretyakov, A.N. and Pantina, R.A. and Kaboev, O.K.},\r\ntitle={Fast DNA Amplification in Tiny Ultrathin Microplates},\r\njournal={Bioorganicheskaya Khimiya},\r\nyear={1997},\r\nvolume={23},\r\nnumber={6},\r\npages={527-528},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347138724&amp;partnerID=40&amp;md5=f26acee6ab5c8d940ffeac0eb2340e22},\r\naffiliation={Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},\r\nabstract={A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler&#x27;s thermoblock. The microplates are made from thin (40-60 μm) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 μl samples and a high velocity of heating and cooling of the thermoblock (up to 7°C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.},\r\nauthor_keywords={Fast DNA amplification;  Microplate;  Polymerase chain reaction},\r\ncorrespondence_address1={Tretyakov, A.N.; Konstantinov Petersburg Inst. N., Russian Academy of Sciences, 188350 Gatchina, Russian Federation},\r\npublisher={Maik Nauka-Interperiodica Publishing},\r\nissn={01323423},\r\npubmed_id={9265475},\r\nlanguage={Russian},\r\nabbrev_source_title={Bioorganicheskaya Khim.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 μm) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 μl samples and a high velocity of heating and cooling of the thermoblock (up to 7°C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1995\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1995')\"\n      onkeypress=\"toggleGroup('group_1995')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1995</span>\n      <span class=\"bibbase_group_count\">\n        \n        (2)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&amp;doi=10.1006%2fbmme.1995.1041&amp;partnerID=40&amp;md5=adda09c8d4cb05b774fbe164b90edd86\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&amp;doi=10.1006%2fbmme.1995.1041&amp;partnerID=40&amp;md5=adda09c8d4cb05b774fbe164b90edd86', event);\">\n    Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Varfolomeeva, E.;  and Ivanov, E.\n</span>\n\n  \n\n</span>\n\n  <i>Biochemical and Molecular Medicine</i>, 55(2): 116-121.  1995.\n  <span class=\"note\">cited By 27</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&amp;doi=10.1006%2fbmme.1995.1041&amp;partnerID=40&amp;md5=adda09c8d4cb05b774fbe164b90edd86\"\n     onclick=\"log_download('filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&amp;doi=10.1006%2fbmme.1995.1041&amp;partnerID=40&amp;md5=adda09c8d4cb05b774fbe164b90edd86', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1006/bmme.1995.1041\"\n     onclick=\"log_download('filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995', 'http://doi.org/10.1006/bmme.1995.1041', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-varfolomeeva-ivanov-flowcytofluorometricdetectionofinflammatoryprocessesbymeasuringrespiratoryburstreactionofperipheralbloodneutrophils-1995')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1995116,\r\nauthor={Filatov, M.V. and Varfolomeeva, E.Y. and Ivanov, E.I.},\r\ntitle={Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils},\r\njournal={Biochemical and Molecular Medicine},\r\nyear={1995},\r\nvolume={55},\r\nnumber={2},\r\npages={116-121},\r\ndoi={10.1006/bmme.1995.1041},\r\nnote={cited By 27},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029151779&amp;doi=10.1006%2fbmme.1995.1041&amp;partnerID=40&amp;md5=adda09c8d4cb05b774fbe164b90edd86},\r\naffiliation={Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},\r\nabstract={This report describes a method for measuring the respiratory burst in neutrophils, based on intracellular oxidation of the reduced ethidium bromide derivative, hydroethidine. Fluorescence of the resultant product quantitatively determined by flow cytofluorometry serves as a measure of the neutrophil ability to generate superoxide radicals. We found that during inflammation some polymorphonuclear leukocytes showed a considerably lower respiratory burst response to phorbol myristate acetate treatment. It was demonstrated that variations in this parameter could be an indicator of the course of inflammation. © 1995 by Academic Press, Inc.},\r\ncorrespondence_address1={Filatov, M.V.; Petersburg Nuclear Physics Institute, St. Petersburg 188 350, Russian Federation},\r\nissn={10773150},\r\nlanguage={English},\r\nabbrev_source_title={BIOCHEM. MOL. MED.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  This report describes a method for measuring the respiratory burst in neutrophils, based on intracellular oxidation of the reduced ethidium bromide derivative, hydroethidine. Fluorescence of the resultant product quantitatively determined by flow cytofluorometry serves as a measure of the neutrophil ability to generate superoxide radicals. We found that during inflammation some polymorphonuclear leukocytes showed a considerably lower respiratory burst response to phorbol myristate acetate treatment. It was demonstrated that variations in this parameter could be an indicator of the course of inflammation. © 1995 by Academic Press, Inc.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&amp;doi=10.1016%2f0027-5107%2894%2900189-C&amp;partnerID=40&amp;md5=f0b6be765868fda9e94902fc88e4bc59\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&amp;doi=10.1016%2f0027-5107%2894%2900189-C&amp;partnerID=40&amp;md5=f0b6be765868fda9e94902fc88e4bc59', event);\">\n    Active dissociation of Hoechst 33342 from DNA in living mammalian cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  <i>Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis</i>, 327(1-2): 209-215.  1995.\n  <span class=\"note\">cited By 20</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&amp;doi=10.1016%2f0027-5107%2894%2900189-C&amp;partnerID=40&amp;md5=f0b6be765868fda9e94902fc88e4bc59\"\n     onclick=\"log_download('anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028986037&amp;doi=10.1016%2f0027-5107%2894%2900189-C&amp;partnerID=40&amp;md5=f0b6be765868fda9e94902fc88e4bc59', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Active dissociation of Hoechst 33342 from DNA in living mammalian cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/0027-5107(94)00189-C\"\n     onclick=\"log_download('anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995', 'http://doi.org/10.1016/0027-5107(94)00189-C', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-activedissociationofhoechst33342fromdnainlivingmammaliancells-1995')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The fluorescent dye Hoechst 33342, which binds tightly to DNA in the minor groove, can be effectively extracted from the DNA in vivo due to an energy-dependent process. It is the dissociation of the dye from DNA rather than its removal from a cell that has a critical role in this process. The dissociation can be suppressed by the topoisomerase-2 inhibitors novobiocin, ellipticine and etoposide. Breaks in the DNA also inhibit the process. The dissociation of the dye requires that DNA remain intact along a region of at least several thousand base pairs. It is proposed that DNA in mammalian cells is a dynamic, conformationally nonstable system and that topologically closed loops consisting of several thousand base pairs constantly appear and disappear in chromatin. © 1995.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1994\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1994')\"\n      onkeypress=\"toggleGroup('group_1994')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1994</span>\n      <span class=\"bibbase_group_count\">\n        \n        (2)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&amp;partnerID=40&amp;md5=68e2dad0fa12c3f6e33f54dd5db8fb1f\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&amp;partnerID=40&amp;md5=68e2dad0fa12c3f6e33f54dd5db8fb1f', event);\">\n    Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Katunin, V.; Soboleva, N.; Makhno, V.; Sedelnikova, E.; Zhenodarova, S.;  and Kirillov, S.\n</span>\n\n  \n\n</span>\n\n  <i>Molekulyarnaya Biologiya</i>, 28(1): 66-75.  1994.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&amp;partnerID=40&amp;md5=68e2dad0fa12c3f6e33f54dd5db8fb1f\"\n     onclick=\"log_download('katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&amp;partnerID=40&amp;md5=68e2dad0fa12c3f6e33f54dd5db8fb1f', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('katunin-soboleva-makhno-sedelnikova-zhenodarova-kirillov-interactionofdeacylatedtrnawiththepsiteofescherichiacoliribosomesroleofmodifiednucleotideincodonanticodoninteraction-1994')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Katunin199466,\r\nauthor={Katunin, V.I. and Soboleva, N.G. and Makhno, V.I. and Sedelnikova, E.A. and Zhenodarova, S.M. and Kirillov, S.V.},\r\ntitle={Interaction of deacylated tRNA with the P site of Escherichia coli ribosomes. Role of modified nucleotide in codon- anticodon interaction},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={1994},\r\nvolume={28},\r\nnumber={1},\r\npages={66-75},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028306695&amp;partnerID=40&amp;md5=68e2dad0fa12c3f6e33f54dd5db8fb1f},\r\naffiliation={St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},\r\nabstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNA((G(m)AAY(Phe)) with the substitution at the 37 position (tRNA(GAAA)(Phe)), and at both the anticodon (tRNA(GCAG)(Phe)) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(G(m)(AAY)(Phe), tRNA(GAAA)(Phe), tRNA(GCAG)(Phe), and tRNA(-Y)(Phe) with the P site of the 70S ribosome·poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalphy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U) the affinity of tRNA(-Y)(Phe) for the P site of the 70S ribosome is 5 times lower that the affinity of tRNA(G(m)AAY(Phe)) and tRNA(GCAG)(Phe). Thus, in the ribosome the mofified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon- anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome.},\r\ncorrespondence_address1={Katunin, V.I.; St. Petersb. Nuclear Physics Inst., Russian Academy of Sciences, Gatchina 188350, Russian Federation},\r\nissn={00268984},\r\ncoden={MOBIB},\r\npubmed_id={8145756},\r\nlanguage={Russian},\r\nabbrev_source_title={MOL. BIOL.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The method of anticodon loop replacement has been used to make derivatives of yeast tRNA((G(m)AAY(Phe)) with the substitution at the 37 position (tRNA(GAAA)(Phe)), and at both the anticodon (tRNA(GCAG)(Phe)) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(G(m)(AAY)(Phe), tRNA(GAAA)(Phe), tRNA(GCAG)(Phe), and tRNA(-Y)(Phe) with the P site of the 70S ribosome·poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalphy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U) the affinity of tRNA(-Y)(Phe) for the P site of the 70S ribosome is 5 times lower that the affinity of tRNA(G(m)AAY(Phe)) and tRNA(GCAG)(Phe). Thus, in the ribosome the mofified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon- anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&amp;doi=10.1016%2f0300-9084%2894%2990062-0&amp;partnerID=40&amp;md5=28e38efb03ea6c49c3f74fdc2487ab60\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&amp;doi=10.1016%2f0300-9084%2894%2990062-0&amp;partnerID=40&amp;md5=28e38efb03ea6c49c3f74fdc2487ab60', event);\">\n    Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Katunin, V.; Soboleva, N.; Mahkno, V.; Sedelnikova, E.; Zhenodarova, S.;  and Kirillov, S.\n</span>\n\n  \n\n</span>\n\n  <i>Biochimie</i>, 76(1): 51-57.  1994.\n  <span class=\"note\">cited By 8</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&amp;doi=10.1016%2f0300-9084%2894%2990062-0&amp;partnerID=40&amp;md5=28e38efb03ea6c49c3f74fdc2487ab60\"\n     onclick=\"log_download('katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&amp;doi=10.1016%2f0300-9084%2894%2990062-0&amp;partnerID=40&amp;md5=28e38efb03ea6c49c3f74fdc2487ab60', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/0300-9084(94)90062-0\"\n     onclick=\"log_download('katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994', 'http://doi.org/10.1016/0300-9084(94)90062-0', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('katunin-soboleva-mahkno-sedelnikova-zhenodarova-kirillov-effectofthenucleotide37ontheinteractionoftrnaphewiththepsiteofescherichiacoliribosomes-1994')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Katunin199451,\r\nauthor={Katunin, V. and Soboleva, N. and Mahkno, V. and Sedelnikova, E. and Zhenodarova, S. and Kirillov, S.},\r\ntitle={Effect of the nucleotide-37 on the interaction of tRNAPhe with the P site of Escherichia coli ribosomes},\r\njournal={Biochimie},\r\nyear={1994},\r\nvolume={76},\r\nnumber={1},\r\npages={51-57},\r\ndoi={10.1016/0300-9084(94)90062-0},\r\nnote={cited By 8},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028213557&amp;doi=10.1016%2f0300-9084%2894%2990062-0&amp;partnerID=40&amp;md5=28e38efb03ea6c49c3f74fdc2487ab60},\r\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation; Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, 142292 Puschino, Moscow Region, Russian Federation},\r\nabstract={The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe with the substitution at position 37 (tRNAGAAAPhe) and at the anticodon(tRNAGCAGPhe). A quantitative study of the interaction of various types of deacylated yeast tRNAPhe (tRNA+YPhe, tRNAGAAAPhe, tRNA-yPhe) with the P site of the [70S ribosome*poly(U)]-complex was carried out at different Mg2+ concentrations and temperatures. The presence and nature of the nucleotide situated at the 3′-end of the anticodon are essential for such interaction in E coli ribosomes. Replacement of thee Y base with the unmodified adenosine decreases the interation enthalpy from 39 kcal/mol to 24 kcal/mol, whereas its removal reduces the interaction enthalpy to 16 kcal/mol. Replacement of the second anticodon nucleotide, adenosine, with cytosine further reduces the enthalphy to 6 kcal/mol, which is typical of tRNA-P site interaction in the absence of poly(U). In the absence of poly(U) the affinity of tRNA-YPhe for the P site of the 70S ribosome is five times lower than the affinity of tRNA+YPhe or tRNAGCAGPhe. Thus, in the ribosome the modified nucleotide stabilizes the codon-anticodon interaction through its stacking interaction with the codon-anticodon base stack. In addition, this decreases the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site. © 1994.},\r\nauthor_keywords={70S ribosome;  codon-anticodon interaction;  Escherichia coli;  modified nucleotide;  tRNAPhe},\r\ncorrespondence_address1={Katunin, V.; Petersburg Nuclear Physics Institute, Russian Academy of Science, 188350 Gatchina, Leningrad Region, Russian Federation},\r\nissn={03009084},\r\ncoden={BICMB},\r\npubmed_id={7518255},\r\nlanguage={English},\r\nabbrev_source_title={Biochimie},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe with the substitution at position 37 (tRNAGAAAPhe) and at the anticodon(tRNAGCAGPhe). A quantitative study of the interaction of various types of deacylated yeast tRNAPhe (tRNA+YPhe, tRNAGAAAPhe, tRNA-yPhe) with the P site of the [70S ribosome*poly(U)]-complex was carried out at different Mg2+ concentrations and temperatures. The presence and nature of the nucleotide situated at the 3′-end of the anticodon are essential for such interaction in E coli ribosomes. Replacement of thee Y base with the unmodified adenosine decreases the interation enthalpy from 39 kcal/mol to 24 kcal/mol, whereas its removal reduces the interaction enthalpy to 16 kcal/mol. Replacement of the second anticodon nucleotide, adenosine, with cytosine further reduces the enthalphy to 6 kcal/mol, which is typical of tRNA-P site interaction in the absence of poly(U). In the absence of poly(U) the affinity of tRNA-YPhe for the P site of the 70S ribosome is five times lower than the affinity of tRNA+YPhe or tRNAGCAGPhe. Thus, in the ribosome the modified nucleotide stabilizes the codon-anticodon interaction through its stacking interaction with the codon-anticodon base stack. In addition, this decreases the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site. © 1994.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1993\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1993')\"\n      onkeypress=\"toggleGroup('group_1993')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1993</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&amp;partnerID=40&amp;md5=07712067d4e466fb44c89e58dfa85128\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&amp;partnerID=40&amp;md5=07712067d4e466fb44c89e58dfa85128', event);\">\n    The production of clones of \"man x Chinese hamster\" hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok \"chelovek x kitaǐskiǐ khomiachok\", soderzhashchikh raznye chasti genoma cheloveka.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Bulat, S.; Drobchenko, E.; Kotlovanova, L.; Pantina, R.; Semenova, E.; Stepanov, S.; Tret'iakov, A.;  and Shcherbakova, O.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 35(6-7): 68-73.  1993.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&amp;partnerID=40&amp;md5=07712067d4e466fb44c89e58dfa85128\"\n     onclick=\"log_download('filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&amp;partnerID=40&amp;md5=07712067d4e466fb44c89e58dfa85128', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The production of clones of &quot;man x Chinese hamster&quot; hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok &quot;chelovek x kitaǐskiǐ khomiachok&quot;, soderzhashchikh raznye chasti genoma cheloveka.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-bulat-drobchenko-kotlovanova-pantina-semenova-stepanov-tretiakov-etal-theproductionofclonesofmanxchinesehamsterhybridcellscontainingdifferentpartsofthehumangenomepoluchenieklonovgibridnykhkletokchelovekxkitaskikhomiachoksoderzhashchikhraznyechastigenomacheloveka-1993')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov199368,\r\nauthor={Filatov, M.V. and Bulat, S.A. and Drobchenko, E.A. and Kotlovanova, L.V. and Pantina, R.A. and Semenova, E.V. and Stepanov, S.I. and Tret&#x27;iakov, A.N. and Shcherbakova, O.G.},\r\ntitle={The production of clones of &quot;man x Chinese hamster&quot; hybrid cells containing different parts of the human genome [Poluchenie klonov gibridnykh kletok &quot;chelovek x kitaǐskiǐ khomiachok&quot;, soderzhashchikh raznye chasti genoma cheloveka.]},\r\njournal={Tsitologiya},\r\nyear={1993},\r\nvolume={35},\r\nnumber={6-7},\r\npages={68-73},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027766095&amp;partnerID=40&amp;md5=07712067d4e466fb44c89e58dfa85128},\r\nabstract={Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={8266566},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&amp;partnerID=40&amp;md5=233809336d07245daae6c4fc6a081fa1\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&amp;partnerID=40&amp;md5=233809336d07245daae6c4fc6a081fa1', event);\">\n    The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal'nymi praǐmerami: vidospetsifichnost' amplifitsirovannoǐ DNK.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Bulat, S.; Filatov, M.;  and Pantina, R.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 35(6-7): 74-78.  1993.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&amp;partnerID=40&amp;md5=233809336d07245daae6c4fc6a081fa1\"\n     onclick=\"log_download('bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&amp;partnerID=40&amp;md5=233809336d07245daae6c4fc6a081fa1', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal&#x27;nymi praǐmerami: vidospetsifichnost&#x27; amplifitsirovannoǐ DNK.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('bulat-filatov-pantina-thedetectionofhumandnaincellhybridsbythepolymerasechainreactionwithuniversalprimersthespeciesspecificityoftheamplifieddnavyiavleniednkchelovekavkletochnykhgibridakhmetodompolimeraznotsepnoreaktsiisuniversalnymiprameramividospetsifichnostamplifitsirovannodnk-1993')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Bulat199374,\r\nauthor={Bulat, S.A. and Filatov, M.V. and Pantina, R.A.},\r\ntitle={The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA [Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoǐ tsepnoǐ reaktsii s universal&#x27;nymi praǐmerami: vidospetsifichnost&#x27; amplifitsirovannoǐ DNK.]},\r\njournal={Tsitologiya},\r\nyear={1993},\r\nvolume={35},\r\nnumber={6-7},\r\npages={74-78},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027759914&amp;partnerID=40&amp;md5=233809336d07245daae6c4fc6a081fa1},\r\nabstract={The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids &quot;human x Chinese hamster&quot; supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.},\r\ncorrespondence_address1={Bulat, S.A.},\r\nissn={00413771},\r\npubmed_id={8266567},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids \"human x Chinese hamster\" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&amp;partnerID=40&amp;md5=a0bd6d6a1eee3ff3c287dde1598ec2f6\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&amp;partnerID=40&amp;md5=a0bd6d6a1eee3ff3c287dde1598ec2f6', event);\">\n    The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel'nosti DARC146.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Luniak, V.; Filatov, M.; Ibatullin, F.;  and Timchenko, N.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 35(5): 84-90.  1993.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&amp;partnerID=40&amp;md5=a0bd6d6a1eee3ff3c287dde1598ec2f6\"\n     onclick=\"log_download('luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&amp;partnerID=40&amp;md5=a0bd6d6a1eee3ff3c287dde1598ec2f6', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel&#x27;nosti DARC146.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('luniak-filatov-ibatullin-timchenko-theinteractionofnuclearproteinswithbentdnalocatedinanautonomouslyreplicatingdarc146sequencevzaimodestvieiadernykhbelkovsizognutodnkraspolozhennovavtonomnoreplitsiruiushchesiaposledovatelnostidarc146-1993')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Luniak199384,\r\nauthor={Luniak, V.V. and Filatov, M.V. and Ibatullin, F.M. and Timchenko, N.A.},\r\ntitle={The interaction of nuclear proteins with bent DNA located in an autonomously replicating DARC146 sequence [Vzaimodeǐstvie iadernykh belkov s izognutoǐ DNK, raspolozhennoǐ v avtonomno replitsiruiushcheǐsia posledovatel&#x27;nosti DARC146.]},\r\njournal={Tsitologiya},\r\nyear={1993},\r\nvolume={35},\r\nnumber={5},\r\npages={84-90},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027343268&amp;partnerID=40&amp;md5=a0bd6d6a1eee3ff3c287dde1598ec2f6},\r\nabstract={The binding of nuclear proteins to a DARC146 DNA fragment is described. The DARC146 was isolated from a complex form of DNA polymerase alpha. BrdUrd substitution experiments indicate that DARC146 can support an autonomous replication in mammalian cells. Three AAA blocks separated by 10 nucleotides were identified in the DARC146 sequence. Measuring electrophoretical mobility under appropriate conditions showed that these AAA blocks form a bent DNA. We have used a synthetic oligonucleotide covering the bent DNA to study the interaction of nuclear proteins with this DNA region. Four DNA-protein complexes with the bent DNA region were registered. One of them is formed by binding nuclear factor p65 to TCTATTA nucleotides. The molecular weight and binding site of p65 are very similar to those of c-myc protein. However, antibodies against c-myc protein exert no effect on the formation of the p65-DNA complex. We suggest that p65 is an unknown nuclear factor.},\r\ncorrespondence_address1={Luniak, V.V.},\r\nissn={00413771},\r\npubmed_id={8379011},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The binding of nuclear proteins to a DARC146 DNA fragment is described. The DARC146 was isolated from a complex form of DNA polymerase alpha. BrdUrd substitution experiments indicate that DARC146 can support an autonomous replication in mammalian cells. Three AAA blocks separated by 10 nucleotides were identified in the DARC146 sequence. Measuring electrophoretical mobility under appropriate conditions showed that these AAA blocks form a bent DNA. We have used a synthetic oligonucleotide covering the bent DNA to study the interaction of nuclear proteins with this DNA region. Four DNA-protein complexes with the bent DNA region were registered. One of them is formed by binding nuclear factor p65 to TCTATTA nucleotides. The molecular weight and binding site of p65 are very similar to those of c-myc protein. However, antibodies against c-myc protein exert no effect on the formation of the p65-DNA complex. We suggest that p65 is an unknown nuclear factor.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1992\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1992')\"\n      onkeypress=\"toggleGroup('group_1992')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1992</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&amp;partnerID=40&amp;md5=cadd0e27379a5b1b7917b210b3fc1a1c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&amp;partnerID=40&amp;md5=cadd0e27379a5b1b7917b210b3fc1a1c', event);\">\n    The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol'nykh revmatoidnym artritom.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Bazhanov, N.; Semenova, E.;  and Ginzburg, L.\n</span>\n\n  \n\n</span>\n\n  <i>Terapevticheskii Arkhiv</i>, 64(2): 93-96.  1992.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&amp;partnerID=40&amp;md5=cadd0e27379a5b1b7917b210b3fc1a1c\"\n     onclick=\"log_download('bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&amp;partnerID=40&amp;md5=cadd0e27379a5b1b7917b210b3fc1a1c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol&#x27;nykh revmatoidnym artritom.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('bazhanov-semenova-ginzburg-theuseofradiothermometryinpatientswithrheumatoidarthritisoprimeneniiradiotermometriiubolnykhrevmatoidnymartritom-1992')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Bazhanov199293,\r\nauthor={Bazhanov, N.N. and Semenova, E.V. and Ginzburg, L.I.},\r\ntitle={The use of radiothermometry in patients with rheumatoid arthritis [O primenenii radiotermometrii u bol&#x27;nykh revmatoidnym artritom.]},\r\njournal={Terapevticheskii Arkhiv},\r\nyear={1992},\r\nvolume={64},\r\nnumber={2},\r\npages={93-96},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026458785&amp;partnerID=40&amp;md5=cadd0e27379a5b1b7917b210b3fc1a1c},\r\nabstract={As a result of the work carried out, one could decide some problems: to develop standardized points of measuring temperatures of the deep structures of the joints using radiothermography, to establish for the first time the normal radiothermographic values of the temperatures for different joint groups, to recommend radiothermography as an adjuvant method for revealing early signs of the activity of rheumatoid arthritis (RA) associated with OA. Radiothermography is a convenient, cheap, available and an informative enough method, fit for the use in clinical practice to improve the diagnosis and treatment of RA.},\r\ncorrespondence_address1={Bazhanov, N.N.},\r\nissn={00403660},\r\npubmed_id={1509395},\r\nlanguage={Russian},\r\nabbrev_source_title={Ter Arkh},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  As a result of the work carried out, one could decide some problems: to develop standardized points of measuring temperatures of the deep structures of the joints using radiothermography, to establish for the first time the normal radiothermographic values of the temperatures for different joint groups, to recommend radiothermography as an adjuvant method for revealing early signs of the activity of rheumatoid arthritis (RA) associated with OA. Radiothermography is a convenient, cheap, available and an informative enough method, fit for the use in clinical practice to improve the diagnosis and treatment of RA.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1991\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1991')\"\n      onkeypress=\"toggleGroup('group_1991')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1991</span>\n      <span class=\"bibbase_group_count\">\n        \n        (2)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&amp;partnerID=40&amp;md5=665c584f8ca0c771026cfbf0048ba0be\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&amp;partnerID=40&amp;md5=665c584f8ca0c771026cfbf0048ba0be', event);\">\n    New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Varfolomeev, D.;  and Kotlovanova, L.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 33(4): 115-120.  1991.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&amp;partnerID=40&amp;md5=665c584f8ca0c771026cfbf0048ba0be\"\n     onclick=\"log_download('filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&amp;partnerID=40&amp;md5=665c584f8ca0c771026cfbf0048ba0be', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-varfolomeev-kotlovanova-newpossibilitiesforstudyingcomplexmacromolecularstructuresofthecellbyencasingcellsinpolyacrylamidemicrospheresnovyevozmozhnostiizucheniiamakromolekuliarnykhkompleksnykhstrukturkletkiputemzakliucheniiakletokvpoliakrilamidnyeshariki-1991')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1991115,\r\nauthor={Filatov, M.V. and Varfolomeev, D.B. and Kotlovanova, L.V.},\r\ntitle={New possibilities for studying complex macromolecular structures of the cell by encasing cells in polyacrylamide microspheres [Novye vozmozhnosti izucheniia makromolekuliarnykh kompleksnykh struktur kletki putem zakliucheniia kletok v poliakrilamidnye shariki.]},\r\njournal={Tsitologiya},\r\nyear={1991},\r\nvolume={33},\r\nnumber={4},\r\npages={115-120},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026311230&amp;partnerID=40&amp;md5=665c584f8ca0c771026cfbf0048ba0be},\r\nabstract={A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={1803700},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A method is proposed for incorporation of mammalian cells into spheres permeable for protein molecules, the dimensions of these spheres not overcoming those of the cells. The method permits fractionation of the cells, preserving them as discrete units resistant to mechanical and hydrodynamic destruction in the course of routine laboratory manipulations, as well as using flow cytometry for their analysis. With this approach, the flow cytometric measurements were made of DNA being shielded in the chromatin complex which prevents from binding fluorescent dyes with low-molecular weights.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&amp;partnerID=40&amp;md5=6def35ea402425f2f367f48691d8c2af\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&amp;partnerID=40&amp;md5=6def35ea402425f2f367f48691d8c2af', event);\">\n    Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost' iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Meerson, F.; Malyshev, I.; Varfolomeeva, E.; Varfolomeev, O.;  and Noskin, A.\n</span>\n\n  \n\n</span>\n\n  <i>Byulleten Eksperimentalnoi Biologii i Meditsiny</i>, 111(5): 460-462.  1991.\n  <span class=\"note\">cited By 4</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&amp;partnerID=40&amp;md5=6def35ea402425f2f367f48691d8c2af\"\n     onclick=\"log_download('meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&amp;partnerID=40&amp;md5=6def35ea402425f2f367f48691d8c2af', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost&#x27; iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('meerson-malyshev-varfolomeeva-varfolomeev-noskin-adaptationofthebodytotheeffectsofstressincreasestheresistanceofcardiacnucleitodamagingeffectsofsinglechainexogenousdnaadaptatsiiaorganizmakstressornymvozdestviiampovyshaetustochivostiaderserdechnykhkletokkpovrezhdaiushchemudestviiuodnonitevokzogennodnk-1991')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Meerson1991460,\r\nauthor={Meerson, F.Z. and Malyshev, I.I. and Varfolomeeva, E.I. and Varfolomeev, O.B. and Noskin, A.N.},\r\ntitle={Adaptation of the body to the effects of stress increases the resistance of cardiac nuclei to damaging effects of single-chain exogenous DNA [Adaptatsiia organizma k stressornym vozdeǐstviiam povyshaet ustoǐchivost&#x27; iader serdechnykh kletok k povrezhdaiushchemu deǐstviiu odnonitevoǐ ékzogennoǐ DNK.]},\r\njournal={Byulleten Eksperimentalnoi Biologii i Meditsiny},\r\nyear={1991},\r\nvolume={111},\r\nnumber={5},\r\npages={460-462},\r\nnote={cited By 4},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026165811&amp;partnerID=40&amp;md5=6def35ea402425f2f367f48691d8c2af},\r\nabstract={The effect of adaptation to stress exposure on the resistance of nuclear DNA to damaging effect of one-chain exogenous DNA was investigated by flow cytometric fluorescence method. It was shown that after the administration of one-chain DNA in concentration of 50 micrograms/ml into the nuclei suspension almost half of nuclear DNA was damaged in control. In adaptation this phenomenon was 5.5 times less pronounced. When the one-chain DNA concentration was increased, this protective effect of adaptation remained. The possible mechanism of the DNA-protective effect of adaptation is under consideration.},\r\ncorrespondence_address1={Meerson, F.Z.},\r\nissn={03659615},\r\npubmed_id={1878555},\r\nlanguage={Russian},\r\nabbrev_source_title={Biull Eksp Biol Med},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The effect of adaptation to stress exposure on the resistance of nuclear DNA to damaging effect of one-chain exogenous DNA was investigated by flow cytometric fluorescence method. It was shown that after the administration of one-chain DNA in concentration of 50 micrograms/ml into the nuclei suspension almost half of nuclear DNA was damaged in control. In adaptation this phenomenon was 5.5 times less pronounced. When the one-chain DNA concentration was increased, this protective effect of adaptation remained. The possible mechanism of the DNA-protective effect of adaptation is under consideration.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1990\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1990')\"\n      onkeypress=\"toggleGroup('group_1990')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1990</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&amp;partnerID=40&amp;md5=954a167f2eb8cd97e225fe80f81d78bc\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&amp;partnerID=40&amp;md5=954a167f2eb8cd97e225fe80f81d78bc', event);\">\n    Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Timchenko, N.; Shcherbakov, O.; Krutyakov, V.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Molekulyarnaya Biologiya</i>, 24(3): 814-823.  1990.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&amp;partnerID=40&amp;md5=954a167f2eb8cd97e225fe80f81d78bc\"\n     onclick=\"log_download('timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&amp;partnerID=40&amp;md5=954a167f2eb8cd97e225fe80f81d78bc', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('timchenko-shcherbakov-krutyakov-filatov-autonomousreplicationofplasmidsbearingdnafragmentsfromratliverpolymerasecomplex-1990')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Timchenko1990814,\r\nauthor={Timchenko, N.A. and Shcherbakov, O.G. and Krutyakov, V.M. and Filatov, M.V.},\r\ntitle={Autonomous replication of plasmids bearing DNA fragments from rat liver α-polymerase complex},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={1990},\r\nvolume={24},\r\nnumber={3},\r\npages={814-823},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025427586&amp;partnerID=40&amp;md5=954a167f2eb8cd97e225fe80f81d78bc},\r\naffiliation={B.P. Konstantinov Nuclear Physics Institute, Academy of Sciences of the USSR, Gatchina, Leningrad Region 188350, Russia},\r\nissn={00268984},\r\ncoden={MOBIB},\r\npubmed_id={2169584},\r\nlanguage={Russian},\r\nabbrev_source_title={MOL. BIOL.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&amp;partnerID=40&amp;md5=b8496824b416bf690816ecd2ca1277da\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&amp;partnerID=40&amp;md5=b8496824b416bf690816ecd2ca1277da', event);\">\n    A simple method for estimating the rate of repair synthesis in cultured mammalian cells.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Streltsov, P.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Radiobiologiya</i>, 30(2): 279-282.  1990.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&amp;partnerID=40&amp;md5=b8496824b416bf690816ecd2ca1277da\"\n     onclick=\"log_download('streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&amp;partnerID=40&amp;md5=b8496824b416bf690816ecd2ca1277da', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A simple method for estimating the rate of repair synthesis in cultured mammalian cells [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('streltsov-filatov-asimplemethodforestimatingtherateofrepairsynthesisinculturedmammaliancells-1990')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Streltsov1990279,\r\nauthor={Streltsov, P.G. and Filatov, M.V.},\r\ntitle={A simple method for estimating the rate of repair synthesis in cultured mammalian cells},\r\njournal={Radiobiologiya},\r\nyear={1990},\r\nvolume={30},\r\nnumber={2},\r\npages={279-282},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025308377&amp;partnerID=40&amp;md5=b8496824b416bf690816ecd2ca1277da},\r\naffiliation={B.P. Konstantinov Leningrad Institute for Nuclear Physics, USSR Academy of Sciences, Gatchina, Russia},\r\nissn={00338192},\r\ncoden={RADOA},\r\npubmed_id={1693442},\r\nlanguage={Russian},\r\nabbrev_source_title={RADIOBIOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&amp;partnerID=40&amp;md5=dfa0e0cab0df064bbdf027d880a7be2b\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&amp;partnerID=40&amp;md5=dfa0e0cab0df064bbdf027d880a7be2b', event);\">\n    A study of chromatin packing in the mammalian cell nuclei by flow cytometry.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.;  and Varfolomeeva Yu., E.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 32(4): 343-351.  1990.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&amp;partnerID=40&amp;md5=dfa0e0cab0df064bbdf027d880a7be2b\"\n     onclick=\"log_download('filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&amp;partnerID=40&amp;md5=dfa0e0cab0df064bbdf027d880a7be2b', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"A study of chromatin packing in the mammalian cell nuclei by flow cytometry [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-varfolomeevayu-astudyofchromatinpackinginthemammaliancellnucleibyflowcytometry-1990')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1990343,\r\nauthor={Filatov, M.V. and Varfolomeeva Yu., E.},\r\ntitle={A study of chromatin packing in the mammalian cell nuclei by flow cytometry},\r\njournal={Tsitologiya},\r\nyear={1990},\r\nvolume={32},\r\nnumber={4},\r\npages={343-351},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025069710&amp;partnerID=40&amp;md5=dfa0e0cab0df064bbdf027d880a7be2b},\r\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Leningrad, Russia},\r\nissn={00413771},\r\ncoden={TSITA},\r\nlanguage={Russian},\r\nabbrev_source_title={TSITOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1989\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1989')\"\n      onkeypress=\"toggleGroup('group_1989')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1989</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&amp;partnerID=40&amp;md5=06c63d8e6d241bf9aeaca3c56b5c6c29\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&amp;partnerID=40&amp;md5=06c63d8e6d241bf9aeaca3c56b5c6c29', event);\">\n    Cell-by-cell chromosomal flow cytometric analysis.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Stepanov, S.; Semenova, E.; Noskin, L.; Drobchenko, E.; Filatov, M.;  and Kotlovanova, L.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 31(4): 410-418.  1989.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&amp;partnerID=40&amp;md5=06c63d8e6d241bf9aeaca3c56b5c6c29\"\n     onclick=\"log_download('stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&amp;partnerID=40&amp;md5=06c63d8e6d241bf9aeaca3c56b5c6c29', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Cell-by-cell chromosomal flow cytometric analysis [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('stepanov-semenova-noskin-drobchenko-filatov-kotlovanova-cellbycellchromosomalflowcytometricanalysis-1989')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Stepanov1989410,\r\nauthor={Stepanov, S.I. and Semenova, E.V. and Noskin, L.A. and Drobchenko, E.A. and Filatov, M.V. and Kotlovanova, L.V.},\r\ntitle={Cell-by-cell chromosomal flow cytometric analysis},\r\njournal={Tsitologiya},\r\nyear={1989},\r\nvolume={31},\r\nnumber={4},\r\npages={410-418},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024538398&amp;partnerID=40&amp;md5=06c63d8e6d241bf9aeaca3c56b5c6c29},\r\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={2756567},\r\nlanguage={Russian},\r\nabbrev_source_title={TSITOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1988\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1988')\"\n      onkeypress=\"toggleGroup('group_1988')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1988</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&amp;partnerID=40&amp;md5=e1990ffa33950ed509f57f13cef44d1a\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&amp;partnerID=40&amp;md5=e1990ffa33950ed509f57f13cef44d1a', event);\">\n    Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil'nost' khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch'iu protochnoǐ tsitometrii.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Kotlovanova, L.; Stepanov, S.; Tret'iakov, A.;  and Strel'tsov, P.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 30(8): 999-1007.  1988.\n  <span class=\"note\">cited By 3</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&amp;partnerID=40&amp;md5=e1990ffa33950ed509f57f13cef44d1a\"\n     onclick=\"log_download('filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&amp;partnerID=40&amp;md5=e1990ffa33950ed509f57f13cef44d1a', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil&#x27;nost&#x27; khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch&#x27;iu protochnoǐ tsitometrii.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-kotlovanova-stepanov-tretiakov-streltsov-reproduciblechromosomalinstabilityofanestablishedchinesehamstercelllinedetectablebyflowcytometryvosproizvodimaianestabilnostkhromosompostoiannoliniikletokkitacheskogokhomiachkavyiavliaemaiaspomoshchiuprotochnotsitometrii-1988')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1988999,\r\nauthor={Filatov, M.V. and Kotlovanova, L.V. and Stepanov, S.I. and Tret&#x27;iakov, A.N. and Strel&#x27;tsov, P.G.},\r\ntitle={Reproducible chromosomal instability of an established Chinese hamster cell line detectable by flow cytometry [Vosproizvodimaia nestabil&#x27;nost&#x27; khromosom postoiannoǐ linii kletok kitaǐcheskogo khomiachka, vyiavliaemaia s pomoshch&#x27;iu protochnoǐ tsitometrii.]},\r\njournal={Tsitologiya},\r\nyear={1988},\r\nvolume={30},\r\nnumber={8},\r\npages={999-1007},\r\nnote={cited By 3},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024066745&amp;partnerID=40&amp;md5=e1990ffa33950ed509f57f13cef44d1a},\r\nabstract={The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={3206547},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1986\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1986')\"\n      onkeypress=\"toggleGroup('group_1986')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1986</span>\n      <span class=\"bibbase_group_count\">\n        \n        (3)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&amp;partnerID=40&amp;md5=8038fcad140efcdbf1a4e95b7d4885ad\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&amp;partnerID=40&amp;md5=8038fcad140efcdbf1a4e95b7d4885ad', event);\">\n    For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.; Pantina, R.;  and Frolova, L.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 28(12): 1364-1368.  1986.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&amp;partnerID=40&amp;md5=8038fcad140efcdbf1a4e95b7d4885ad\"\n     onclick=\"log_download('filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&amp;partnerID=40&amp;md5=8038fcad140efcdbf1a4e95b7d4885ad', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-pantina-frolova-forthereproductivedeathofacelldamagetoasinglechromosomeissufficientdliareproduktivnogibelikletkidostachnopovrezhdeniiaodnokhromosomy-1986')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov19861364,\r\nauthor={Filatov, M.V. and Pantina, R.A. and Frolova, L.I.},\r\ntitle={For the reproductive death of a cell, damage to a single chromosome is sufficient [Dlia reproduktivnoǐ gibeli kletki dostachno povrezhdeniia odnoǐ khromosomy.]},\r\njournal={Tsitologiya},\r\nyear={1986},\r\nvolume={28},\r\nnumber={12},\r\npages={1364-1368},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022962951&amp;partnerID=40&amp;md5=8038fcad140efcdbf1a4e95b7d4885ad},\r\nabstract={The Chinese hamster cells V-79 were treated with BUdR during one cell cycle; after that the cells were grown in the medium without BUdR and were irradiated by longwave-UV-light at different time. The cell survival after photolysis was compared with the percentage of metaphase plates with different number of chromosomes containing BUdR. It is concluded that for cell inactivation the presence of only one destroyed chromosome (or its part) is enough.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={3824524},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The Chinese hamster cells V-79 were treated with BUdR during one cell cycle; after that the cells were grown in the medium without BUdR and were irradiated by longwave-UV-light at different time. The cell survival after photolysis was compared with the percentage of metaphase plates with different number of chromosomes containing BUdR. It is concluded that for cell inactivation the presence of only one destroyed chromosome (or its part) is enough.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&amp;partnerID=40&amp;md5=c5801fa59eddabd92ec6257a507b8d26\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&amp;partnerID=40&amp;md5=c5801fa59eddabd92ec6257a507b8d26', event);\">\n    Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Stepanova, S.; Vershilova, E.; Malinovskiǐ, O.;  and Filatov, M.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiia</i>, 28(5): 583-586.  1986.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&amp;partnerID=40&amp;md5=c5801fa59eddabd92ec6257a507b8d26\"\n     onclick=\"log_download('stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&amp;partnerID=40&amp;md5=c5801fa59eddabd92ec6257a507b8d26', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('stepanova-vershilova-malinovski-filatov-analysisofthechromosomenumberincellsbyflowcytometryanalizkolichestvakhromosomvkletkakhmetodomprotochnotsitometrii-1986')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Stepanova1986583,\r\nauthor={Stepanova, S.I. and Vershilova, E.I. and Malinovskiǐ, O.V. and Filatov, M.V.},\r\ntitle={Analysis of the chromosome number in cells by flow cytometry [Analiz kolichestva khromosom v kletkakh metodom protochnoǐ tsitometrii.]},\r\njournal={Tsitologiia},\r\nyear={1986},\r\nvolume={28},\r\nnumber={5},\r\npages={583-586},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022709954&amp;partnerID=40&amp;md5=c5801fa59eddabd92ec6257a507b8d26},\r\nabstract={A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.},\r\ncorrespondence_address1={Stepanova, S.I.},\r\nissn={00413771},\r\npubmed_id={3738992},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&amp;partnerID=40&amp;md5=e0eb3de06bc13b567cab07d6a39ce3c5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&amp;partnerID=40&amp;md5=e0eb3de06bc13b567cab07d6a39ce3c5', event);\">\n    Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Semenova, E.; Maximov, V.; Volokitina, M.;  and Egorov, N.\n</span>\n\n  \n\n</span>\n\n  <i>Mikrobiologiya</i>, 55(4): 643-647.  1986.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&amp;partnerID=40&amp;md5=e0eb3de06bc13b567cab07d6a39ce3c5\"\n     onclick=\"log_download('semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&amp;partnerID=40&amp;md5=e0eb3de06bc13b567cab07d6a39ce3c5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('semenova-maximov-volokitina-egorov-optimizationofthemediumandtheeffectofitscomponentsonexopolysaccharidebiosynthesisbymycobacteriumcyaneum-1986')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Semenova1986643,\r\nauthor={Semenova, E.V. and Maximov, V.N. and Volokitina, M.V. and Egorov, N.S.},\r\ntitle={Optimization of the medium and the effect of its components on exopolysaccharide biosynthesis by Mycobacterium cyaneum},\r\njournal={Mikrobiologiya},\r\nyear={1986},\r\nvolume={55},\r\nnumber={4},\r\npages={643-647},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022908207&amp;partnerID=40&amp;md5=e0eb3de06bc13b567cab07d6a39ce3c5},\r\naffiliation={Moskovskij Gosudarstvennyj Universitet Biologicheskij Fakul&#x27;tet, Moskva, Russian Federation},\r\nabstract={The effect of some components of the medium on the growth of Mycobacterium cyaneum B-646 and on the biosynthesis of an exoglycan by the culture was studied. A medium in which the M. cyaneum M variant produced up to 2.2 g of the exopolysaccharide per litre, which was nearly 4 times more than in the original medium, was proposed. The new medium differed from the original one in an elevated content of carbon, iron and potassium (11.2, 5.0 and 1.4 times, respectively) and in a lower phosphorus content (6.7 times). The exopolysaccharide produced by the culture in this medium contained glucose, galactose, fucose and uronic acid. Therefore, its monomeric composition did not depend on the medium used for growing the culture which produced the exopolysaccharide.},\r\nissn={00263656},\r\ncoden={MIKBA},\r\npubmed_id={3773795},\r\nlanguage={Russian},\r\nabbrev_source_title={MIKROBIOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The effect of some components of the medium on the growth of Mycobacterium cyaneum B-646 and on the biosynthesis of an exoglycan by the culture was studied. A medium in which the M. cyaneum M variant produced up to 2.2 g of the exopolysaccharide per litre, which was nearly 4 times more than in the original medium, was proposed. The new medium differed from the original one in an elevated content of carbon, iron and potassium (11.2, 5.0 and 1.4 times, respectively) and in a lower phosphorus content (6.7 times). The exopolysaccharide produced by the culture in this medium contained glucose, galactose, fucose and uronic acid. Therefore, its monomeric composition did not depend on the medium used for growing the culture which produced the exopolysaccharide.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1984\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1984')\"\n      onkeypress=\"toggleGroup('group_1984')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1984</span>\n      <span class=\"bibbase_group_count\">\n        \n        (5)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&amp;partnerID=40&amp;md5=dbd6284fdad0eebea33f4423d212ddd6\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&amp;partnerID=40&amp;md5=dbd6284fdad0eebea33f4423d212ddd6', event);\">\n    Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Bresler, S.; Davidenkova, E.; Filatov, M.; Shvarts, E.;  and Noskin, L.\n</span>\n\n  \n\n</span>\n\n  <i>Radiobiologiya</i>, 24(3): 291-295.  1984.\n  <span class=\"note\">cited By 2</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&amp;partnerID=40&amp;md5=dbd6284fdad0eebea33f4423d212ddd6\"\n     onclick=\"log_download('bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&amp;partnerID=40&amp;md5=dbd6284fdad0eebea33f4423d212ddd6', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('bresler-davidenkova-filatov-shvarts-noskin-determinationofthelevelofspontaneousdamageinhumanandmammaliancelldnaopredelenieurovniaspontannykhpovrezhdenidnkkletokchelovekaimlekopitaiushchikh-1984')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Bresler1984291,\r\nauthor={Bresler, S.E. and Davidenkova, E.F. and Filatov, M.V. and Shvarts, E.I. and Noskin, L.A.},\r\ntitle={Determination of the level of spontaneous damage in human and mammalian cell DNA [Opredelenie urovnia spontannykh povrezhdeniǐ DNK kletok cheloveka i mlekopitaiushchikh.]},\r\njournal={Radiobiologiya},\r\nyear={1984},\r\nvolume={24},\r\nnumber={3},\r\npages={291-295},\r\nnote={cited By 2},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021434247&amp;partnerID=40&amp;md5=dbd6284fdad0eebea33f4423d212ddd6},\r\nabstract={A technique for registration of spontaneous DNA lesions in intact mammalian cells is proposed. Arabinosid cytosine and hydroxyurea were shown to inhibit the repair of gaps spontaneously formed and accumulated in DNA leading to the formation of double-strand DNA breaks and cell death. The rate of spontaneous lesions was different in cells from different animal species and different tissues. A considerable increase in the level of spontaneous lesions was noted with ageing. It is concluded that the level of spontaneous DNA lesions is one of the fundamental biologically important characteristics of a cell.},\r\ncorrespondence_address1={Bresler, S.E.},\r\nissn={00338192},\r\npubmed_id={6739729},\r\nlanguage={Russian},\r\nabbrev_source_title={Radiobiologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A technique for registration of spontaneous DNA lesions in intact mammalian cells is proposed. Arabinosid cytosine and hydroxyurea were shown to inhibit the repair of gaps spontaneously formed and accumulated in DNA leading to the formation of double-strand DNA breaks and cell death. The rate of spontaneous lesions was different in cells from different animal species and different tissues. A considerable increase in the level of spontaneous lesions was noted with ageing. It is concluded that the level of spontaneous DNA lesions is one of the fundamental biologically important characteristics of a cell.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&amp;partnerID=40&amp;md5=993c307758a8f3695b9b6f979ad06bf8\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&amp;partnerID=40&amp;md5=993c307758a8f3695b9b6f979ad06bf8', event);\">\n    New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.;  and Sheǐkina, T.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 26(4): 450-457.  1984.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&amp;partnerID=40&amp;md5=993c307758a8f3695b9b6f979ad06bf8\"\n     onclick=\"log_download('filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&amp;partnerID=40&amp;md5=993c307758a8f3695b9b6f979ad06bf8', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-shekina-newmethodforstudyingthekineticsoftheexcisionrepairofdnadamagesinmammaliancellsnovysposobissledovaniiakinetikikstsizionnoreparatsiipovrezhdenidnkvkletkakhmlekopitaiushchikh-1984')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1984450,\r\nauthor={Filatov, M.V. and Sheǐkina, T.A.},\r\ntitle={New method for studying the kinetics of the excision repair of DNA damages in mammalian cells [Novyǐ sposob issledovaniia kinetiki ékstsizionnoǐ reparatsii povrezhdeniǐ DNK v kletkakh mlekopitaiushchikh.]},\r\njournal={Tsitologiya},\r\nyear={1984},\r\nvolume={26},\r\nnumber={4},\r\npages={450-457},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021414654&amp;partnerID=40&amp;md5=993c307758a8f3695b9b6f979ad06bf8},\r\nabstract={A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.},\r\ncorrespondence_address1={Filatov, M.V.},\r\nissn={00413771},\r\npubmed_id={6740764},\r\nlanguage={Russian},\r\nabbrev_source_title={Tsitologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&amp;partnerID=40&amp;md5=8308484a481aa0f50924b9af171d5084\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&amp;partnerID=40&amp;md5=8308484a481aa0f50924b9af171d5084', event);\">\n    Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Egorov, N.; Grechushkina, N.; Botvinko, I.; Sviridov, A.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Mikrobiologiya</i>, 53(2): 199-202.  1984.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&amp;partnerID=40&amp;md5=8308484a481aa0f50924b9af171d5084\"\n     onclick=\"log_download('egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&amp;partnerID=40&amp;md5=8308484a481aa0f50924b9af171d5084', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('egorov-grechushkina-botvinko-sviridov-semenova-extracellularpolysaccharidesofsaprotrophicmycobacteriaandpatternsoftheirformationvnekletochnyepolisakharidysaprotrofnykhmikobakteriinekotoryezakonomernostiikhobrazovaniia-1984')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Egorov1984199,\r\nauthor={Egorov, N.S. and Grechushkina, N.N. and Botvinko, I.V. and Sviridov, A.F. and Semenova, E.V.},\r\ntitle={Extracellular polysaccharides of saprotrophic mycobacteria and patterns of their formation [Vnekletochnye polisakharidy saprotrofnykh mikobakteriǐ i nekotorye zakonomernosti ikh obrazovaniia.]},\r\njournal={Mikrobiologiya},\r\nyear={1984},\r\nvolume={53},\r\nnumber={2},\r\npages={199-202},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021400490&amp;partnerID=40&amp;md5=8308484a481aa0f50924b9af171d5084},\r\nabstract={Exocellular polysaccharides were synthesized by ten strains of 7 saprotrophic mycobacterial species in parallel with their growth. The cultures grew and produced polysaccharides more rapidly in a medium with glucose than in a medium with n-hexadecane. The polymers are homogeneous acid heteroglycans of the regular structure. All of them contain glucose and some of them contain hexuronic acid. Galactose, fucose, mannose, ramnose and pyruvic acid are found in some of the polysaccharides. The exoglycans of two mycobacterial species contain mannolactylic acid. Beta-glycosidic linkages prevail in most of the polysaccharides.},\r\ncorrespondence_address1={Egorov, N.S.},\r\nissn={00263656},\r\npubmed_id={6738386},\r\nlanguage={Russian},\r\nabbrev_source_title={Mikrobiologiia},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Exocellular polysaccharides were synthesized by ten strains of 7 saprotrophic mycobacterial species in parallel with their growth. The cultures grew and produced polysaccharides more rapidly in a medium with glucose than in a medium with n-hexadecane. The polymers are homogeneous acid heteroglycans of the regular structure. All of them contain glucose and some of them contain hexuronic acid. Galactose, fucose, mannose, ramnose and pyruvic acid are found in some of the polysaccharides. The exoglycans of two mycobacterial species contain mannolactylic acid. Beta-glycosidic linkages prevail in most of the polysaccharides.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&amp;partnerID=40&amp;md5=bbdd1d28eb7da8606eae844f947424da\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&amp;partnerID=40&amp;md5=bbdd1d28eb7da8606eae844f947424da', event);\">\n    Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.;  and Sheikina, T.\n</span>\n\n  \n\n</span>\n\n  <i>Tsitologiya</i>, 26(10): 1208-1212.  1984.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&amp;partnerID=40&amp;md5=bbdd1d28eb7da8606eae844f947424da\"\n     onclick=\"log_download('filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&amp;partnerID=40&amp;md5=bbdd1d28eb7da8606eae844f947424da', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-sheikina-caffeineinactivationofmammaliancellsinsphaseofmitoticcycleduetoanomalousinitiationofrepliconsduringinhibitionofreplicatingdnachainelongation-1984')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov19841208,\r\nauthor={Filatov, M.V. and Sheikina, T.A.},\r\ntitle={Caffeine inactivation of mammalian cells in S-phase of mitotic cycle due to anomalous initiation of replicons during inhibition of replicating DNA chain elongation},\r\njournal={Tsitologiya},\r\nyear={1984},\r\nvolume={26},\r\nnumber={10},\r\npages={1208-1212},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021509373&amp;partnerID=40&amp;md5=bbdd1d28eb7da8606eae844f947424da},\r\naffiliation={Leningrad Institute of Nuclear Physics of the Academy of Sciences of the USSR, Gatchina, Russian Federation},\r\nissn={00413771},\r\ncoden={TSITA},\r\npubmed_id={6515721},\r\nlanguage={Russian},\r\nabbrev_source_title={TSITOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&amp;partnerID=40&amp;md5=58e4dbe1068fbdb5fb8908eaf0c1738c\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&amp;partnerID=40&amp;md5=58e4dbe1068fbdb5fb8908eaf0c1738c', event);\">\n    Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul'tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Botvinko, I.; Grechushkina, N.; Rachinskaia, L.; Semenova, E.;  and Egorov, N.\n</span>\n\n  \n\n</span>\n\n  <i>Nauchnye doklady vysshei shkoly. Biologicheskie nauki</i>, (2): 89-92.  1984.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&amp;partnerID=40&amp;md5=58e4dbe1068fbdb5fb8908eaf0c1738c\"\n     onclick=\"log_download('botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&amp;partnerID=40&amp;md5=58e4dbe1068fbdb5fb8908eaf0c1738c', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul&#x27;tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('botvinko-grechushkina-rachinskaia-semenova-egorov-effectofcultureconditionsonmycobacteriumcyaneumgrowthandtheformationofextracellularpolysaccharidevliianieuslovikultivirovaniianarostmycobacteriumcyaneumiobrazovanievnekletochnogopolisakharida-1984')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Botvinko198489,\r\nauthor={Botvinko, I.V. and Grechushkina, N.N. and Rachinskaia, L.G. and Semenova, E.V. and Egorov, N.S.},\r\ntitle={Effect of culture conditions on Mycobacterium cyaneum growth and the formation of extracellular polysaccharide [Vliianie usloviǐ kul&#x27;tivirovaniia na rost Mycobacterium cyaneum i obrazovanie vnekletochnogo polisakharida.]},\r\njournal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},\r\nyear={1984},\r\nnumber={2},\r\npages={89-92},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021296166&amp;partnerID=40&amp;md5=58e4dbe1068fbdb5fb8908eaf0c1738c},\r\nabstract={The source of carbon and nitrogen as well as pH of medium influence the possibility of synthesis of the exocellular heteropolysaccharide of Mycobacterium cyaneum and the quantitative harvest of glucan but not its monomer composition. The conditions of mycobacterial growth and glucan synthesis usually do not coincide.},\r\ncorrespondence_address1={Botvinko, I.V.},\r\nissn={04704606},\r\npubmed_id={6713026},\r\nlanguage={Russian},\r\nabbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The source of carbon and nitrogen as well as pH of medium influence the possibility of synthesis of the exocellular heteropolysaccharide of Mycobacterium cyaneum and the quantitative harvest of glucan but not its monomer composition. The conditions of mycobacterial growth and glucan synthesis usually do not coincide.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1983\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1983')\"\n      onkeypress=\"toggleGroup('group_1983')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1983</span>\n      <span class=\"bibbase_group_count\">\n        \n        (2)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&amp;doi=10.1007%2fBF00830178&amp;partnerID=40&amp;md5=2d05f6b733ea895fd47478957ed6c488\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&amp;doi=10.1007%2fBF00830178&amp;partnerID=40&amp;md5=2d05f6b733ea895fd47478957ed6c488', event);\">\n    Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Oleinikova, E.; Egorov, N.; Anoshkina, G.; Morozov, A.; Botvinko, I.;  and Semenova, E.\n</span>\n\n  \n\n</span>\n\n  <i>Bulletin of Experimental Biology and Medicine</i>, 95(3): 320-323.  1983.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&amp;doi=10.1007%2fBF00830178&amp;partnerID=40&amp;md5=2d05f6b733ea895fd47478957ed6c488\"\n     onclick=\"log_download('oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&amp;doi=10.1007%2fBF00830178&amp;partnerID=40&amp;md5=2d05f6b733ea895fd47478957ed6c488', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1007/BF00830178\"\n     onclick=\"log_download('oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983', 'http://doi.org/10.1007/BF00830178', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('oleinikova-egorov-anoshkina-morozov-botvinko-semenova-mechanismofactionoftheexopolysaccharideofmycobacteriumcyaneumonthelocalcellreactioninexperimentalecoliinfection-1983')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Oleinikova1983320,\r\nauthor={Oleinikova, E.A. and Egorov, N.S. and Anoshkina, G.B. and Morozov, A.T. and Botvinko, I.V. and Semenova, E.V.},\r\ntitle={Mechanism of action of the exopolysaccharide of Mycobacterium cyaneum on the local cell reaction in experimental E. coli infection},\r\njournal={Bulletin of Experimental Biology and Medicine},\r\nyear={1983},\r\nvolume={95},\r\nnumber={3},\r\npages={320-323},\r\ndoi={10.1007/BF00830178},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250153766&amp;doi=10.1007%2fBF00830178&amp;partnerID=40&amp;md5=2d05f6b733ea895fd47478957ed6c488},\r\nauthor_keywords={cell enzymes;  cell reaction;  macrophages;  mycobacterial polysaccharide;  phagocytosis},\r\npublisher={Kluwer Academic Publishers-Plenum Publishers},\r\nissn={00074888},\r\ncoden={BEXBA},\r\nlanguage={English},\r\nabbrev_source_title={Bull Exp Biol Med},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&amp;doi=10.1016%2f0027-5107%2883%2990155-0&amp;partnerID=40&amp;md5=618e32c711de3df565c57dec8399c8d5\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&amp;doi=10.1016%2f0027-5107%2883%2990155-0&amp;partnerID=40&amp;md5=618e32c711de3df565c57dec8399c8d5', event);\">\n    Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.;  and Noskin, L.\n</span>\n\n  \n\n</span>\n\n  <i>Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis</i>, 110(2): 393-399.  1983.\n  <span class=\"note\">cited By 6</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&amp;doi=10.1016%2f0027-5107%2883%2990155-0&amp;partnerID=40&amp;md5=618e32c711de3df565c57dec8399c8d5\"\n     onclick=\"log_download('filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&amp;doi=10.1016%2f0027-5107%2883%2990155-0&amp;partnerID=40&amp;md5=618e32c711de3df565c57dec8399c8d5', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1016/0027-5107(83)90155-0\"\n     onclick=\"log_download('filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983', 'http://doi.org/10.1016/0027-5107(83)90155-0', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-noskin-sensitizationofhumancellsbyinhibitorsofdnasynthesisfollowingtheactionofdnadamagingagents-1983')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1983393,\r\nauthor={Filatov, M.V. and Noskin, L.A.},\r\ntitle={Sensitization of human cells by inhibitors of DNA synthesis following the action of DNA-damaging agents},\r\njournal={Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis},\r\nyear={1983},\r\nvolume={110},\r\nnumber={2},\r\npages={393-399},\r\ndoi={10.1016/0027-5107(83)90155-0},\r\nnote={cited By 6},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020576657&amp;doi=10.1016%2f0027-5107%2883%2990155-0&amp;partnerID=40&amp;md5=618e32c711de3df565c57dec8399c8d5},\r\naffiliation={Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},\r\nabstract={Inhibitors of DNA synthesis 1-β-arabinofuranosylcytosine (Ac) and hydroxyurea (Hu) taken together drastically sensitized human cells to the killing effect of DNA-damaging agents. For UV-irradiation this sensitization depended on the cells′ ability for excision repair. By using viscoelastometric methods of measurement of double-strand breaks (DSB) in the genome, it was established that the first DSB were generated after incubation of the damaged cells in the mixture of inhibitors at about the same dose when sensitization appeared. A scheme is proposed to described molecular events associated with the phenomenon studied. © 1983.},\r\ncorrespondence_address1={Filatov, M.V.; Leningrad Institute of Nuclear Physics, Gatchina, 188350 Leningrad District, Russian Federation},\r\nissn={00275107},\r\ncoden={MRFME},\r\npubmed_id={6877262},\r\nlanguage={English},\r\nabbrev_source_title={Mutat. Res. Fundam. Mol. Mech. Mutagen.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Inhibitors of DNA synthesis 1-β-arabinofuranosylcytosine (Ac) and hydroxyurea (Hu) taken together drastically sensitized human cells to the killing effect of DNA-damaging agents. For UV-irradiation this sensitization depended on the cells′ ability for excision repair. By using viscoelastometric methods of measurement of double-strand breaks (DSB) in the genome, it was established that the first DSB were generated after incubation of the damaged cells in the mixture of inhibitors at about the same dose when sensitization appeared. A scheme is proposed to described molecular events associated with the phenomenon studied. © 1983.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1982\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1982')\"\n      onkeypress=\"toggleGroup('group_1982')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1982</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&amp;partnerID=40&amp;md5=e6f60aadab293185ae5595d6bf4bebdf\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&amp;partnerID=40&amp;md5=e6f60aadab293185ae5595d6bf4bebdf', event);\">\n    Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Belyakova, N.; Naryzhnyi, S.; Filatov, M.;  and Krutyakov, V.\n</span>\n\n  \n\n</span>\n\n  <i>Radiobiologiya</i>, 22(5): 593-596.  1982.\n  <span class=\"note\">cited By 1</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&amp;partnerID=40&amp;md5=e6f60aadab293185ae5595d6bf4bebdf\"\n     onclick=\"log_download('belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&amp;partnerID=40&amp;md5=e6f60aadab293185ae5595d6bf4bebdf', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('belyakova-naryzhnyi-filatov-krutyakov-enzymicandstructuralaspectsofrepairdnasynthesisactivationinmammalianchromatin-1982')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Belyakova1982593,\r\nauthor={Belyakova, N.V. and Naryzhnyi, S.N. and Filatov, M.V. and Krutyakov, V.M.},\r\ntitle={Enzymic and structural aspects of repair DNA synthesis activation in mammalian chromatin},\r\njournal={Radiobiologiya},\r\nyear={1982},\r\nvolume={22},\r\nnumber={5},\r\npages={593-596},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020448912&amp;partnerID=40&amp;md5=e6f60aadab293185ae5595d6bf4bebdf},\r\naffiliation={B.P. Konstantinov Leningrad Inst. Nucl. Phys., USSR Acad. Sci., Gatchina},\r\nabstract={Analysis was made of the enzymic and structural factors responsible for activation of repair DNA synthesis in γ-irradiated chromatin isolated from rat liver or some human cells. The results obtained prompted us to reduce by 10-12 times the dose of radiation used before. With doses of 30 Gy and 10 Gy, the value of the original repair synthesis was doubled in the chromatin of rat liver and HeLa cells, respectively.},\r\nissn={00338192},\r\ncoden={RADOA},\r\npubmed_id={6294723},\r\nlanguage={Russian},\r\nabbrev_source_title={RADIOBIOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Analysis was made of the enzymic and structural factors responsible for activation of repair DNA synthesis in γ-irradiated chromatin isolated from rat liver or some human cells. The results obtained prompted us to reduce by 10-12 times the dose of radiation used before. With doses of 30 Gy and 10 Gy, the value of the original repair synthesis was doubled in the chromatin of rat liver and HeLa cells, respectively.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1981\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1981')\"\n      onkeypress=\"toggleGroup('group_1981')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1981</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&amp;partnerID=40&amp;md5=2e1d7f3a9923aa76ca56ba13cbc0989d\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&amp;partnerID=40&amp;md5=2e1d7f3a9923aa76ca56ba13cbc0989d', event);\">\n    Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.].\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Botvinko, I.; Grechushkina, N.; Semenova, E.;  and Egorov, N.\n</span>\n\n  \n\n</span>\n\n  <i>Nauchnye doklady vysshei shkoly. Biologicheskie nauki</i>, (4): 95-99.  1981.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&amp;partnerID=40&amp;md5=2e1d7f3a9923aa76ca56ba13cbc0989d\"\n     onclick=\"log_download('botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&amp;partnerID=40&amp;md5=2e1d7f3a9923aa76ca56ba13cbc0989d', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.] [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('botvinko-grechushkina-semenova-egorov-localizationofextracellularpolysaccharidesynthesisinmycobacteriumlacticolumolokalizatsiisintezavnekletochnogopolisakharidamycobacteriumlacticolum-1981')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Botvinko198195,\r\nauthor={Botvinko, I.V. and Grechushkina, N.N. and Semenova, E.V. and Egorov, N.S.},\r\ntitle={Localization of extracellular polysaccharide synthesis in Mycobacterium lacticolum [O lokalizatsii sinteza vnekletochnogo polisakharida Mycobacterium lacticolum.]},\r\njournal={Nauchnye doklady vysshei shkoly. Biologicheskie nauki},\r\nyear={1981},\r\nnumber={4},\r\npages={95-99},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019400521&amp;partnerID=40&amp;md5=2e1d7f3a9923aa76ca56ba13cbc0989d},\r\ncorrespondence_address1={Botvinko, I.V.},\r\nissn={04704606},\r\npubmed_id={7236813},\r\nlanguage={Russian},\r\nabbrev_source_title={Nauchnye Doki Vyss Shkoly Biol Nauki},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n</pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_1978\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_1978')\"\n      onkeypress=\"toggleGroup('group_1978')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>1978</span>\n      <span class=\"bibbase_group_count\">\n        \n        (1)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&amp;partnerID=40&amp;md5=4218fc5755a7f1beb190156f709214c7\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&amp;partnerID=40&amp;md5=4218fc5755a7f1beb190156f709214c7', event);\">\n    Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation.\n  </a>\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  Filatov, M.;  and Sheikina, T.\n</span>\n\n  \n\n</span>\n\n  <i>Radiobiologiya</i>, 18(5): 751-754.  1978.\n  <span class=\"note\">cited By 0</span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&amp;partnerID=40&amp;md5=4218fc5755a7f1beb190156f709214c7\"\n     onclick=\"log_download('filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978', 'https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&amp;partnerID=40&amp;md5=4218fc5755a7f1beb190156f709214c7', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('filatov-sheikina-interphasedeathofhelazh63cellsinducedbyhighdosesofradiation-1978')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@ARTICLE{Filatov1978751,\r\nauthor={Filatov, M.V. and Sheikina, T.A.},\r\ntitle={Interphase death of HeLa ZH-63 cells induced by high doses of γ-radiation},\r\njournal={Radiobiologiya},\r\nyear={1978},\r\nvolume={18},\r\nnumber={5},\r\npages={751-754},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0018009903&amp;partnerID=40&amp;md5=4218fc5755a7f1beb190156f709214c7},\r\naffiliation={B.P. Konstantinov Inst. Nucl. Phys., USSR Acad. Sci., Leningrad},\r\nabstract={Interphase death of HeLa cells was assessed by the ability of cells to be stained with trypan blue after γ-irradiation with doses from 50 to 2000 krad. A quantitative relationship between average life span of cells and radiation dose has been shown. A decrease of the postirradiation temperature and an increase of the serum content in the growth medium before irradiation have been found to delay the interphase death. Adhesive properties of cells were damaged immediately after exposure.},\r\nissn={00338192},\r\ncoden={RADOA},\r\npubmed_id={715202},\r\nlanguage={Russian},\r\nabbrev_source_title={RADIOBIOLOGIYA},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Interphase death of HeLa cells was assessed by the ability of cells to be stained with trypan blue after γ-irradiation with doses from 50 to 2000 krad. A quantitative relationship between average life span of cells and radiation dose has been shown. A decrease of the postirradiation temperature and an increase of the serum content in the growth medium before irradiation have been found to delay the interphase death. Adhesive properties of cells were damaged immediately after exposure.\n</div>\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n  <div class=\"bibbase_group_whole\" id=\"group_undefined\">\n    <div class=\"bibbase_group\"\n      onclick=\"toggleGroup('group_undefined')\"\n      onkeypress=\"toggleGroup('group_undefined')\"\n      tabindex=\"0\">\n      <i class=\"fa fa-angle-down bibbase_group_head_icon\"></i>&nbsp;\n      <span>undefined</span>\n      <span class=\"bibbase_group_count\">\n        \n        (7)\n        \n      </span>\n    </div>\n    <div class=\"bibbase_group_body\">\n      \n  \n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"anonymous-\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  .\n  \n  \n  \n</span>\n\n  <span class=\"bibbase_paper_author\">\n  \n</span>\n\n  \n\n</span>\n\n  .  .\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/anonymous-\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>9 downloads</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('anonymous-')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\"></pre>\n</div>\n\n\n\n</div>\n\n\n\n\n\n    </div>\n  </div>\n\n\n\n\n  </div>\n\n\n  <!-- Modal -->\n\n  <!-- <iframe id=\"bibbase_network_frame\" -->\n  <!--         src=\"//bibbase.org/network/control\" -->\n  <!--         style=\"display: none;\"> -->\n  <!-- </iframe> -->\n\n</div>\n"}; document.write(bibbase_data.data);