Fast determination of prominent carotenoids in tomato fruits by CEC using methacrylate ester-based monolithic columns. Adalid, A., Herrero-Martínez, J., Roselló, S., Maquieira, A., & Nuez, F. Electrophoresis, 28(22):4120-4127, Wiley-VCH Verlag, 2007. cited By 14Paper doi abstract bibtex In this study, the major carotenoids (β-carotene and lycopene) present in tomato fruits were analyzed by CEC with a methacrylate ester-based monolithic column. The effects of the porogenic solvent ratio, and the hydrophobicity of bulk monomer employed were examined on carotenoids separations. A fast separation of these analytes was achieved in less than 5.0 min in a mobile phase containing 35% THF, 30% ACN, 30% methanol, and 5% of a 5 mM Tris aqueous buffer, pH 8, with lauryl methacrylate-based monoliths. The CEC method was evaluated in terms of detection limit and reproducibility (retention time, area, and column preparation) with values below 1.6 μg/mL and 7.2%, respectively. The proposed procedure was successfully applied to the determination of both carotenoids in fruits of several tomato-related species and its usefulness to analyze large series of samples for nutritional quality screening trials in tomato breeding programs is demonstrated. To our knowledge, this is the first work that exploits the powerful and user-friendly monolithic technology for quality breeding and germplasm evaluation program purposes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
@ARTICLE{Adalid20074120,
author={Adalid, A.M. and Herrero-Martínez, J.M. and Roselló, S. and Maquieira, A. and Nuez, F.},
title={Fast determination of prominent carotenoids in tomato fruits by CEC using methacrylate ester-based monolithic columns},
journal={Electrophoresis},
year={2007},
volume={28},
number={22},
pages={4120-4127},
doi={10.1002/elps.200600775},
note={cited By 14},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36549071138&doi=10.1002%2felps.200600775&partnerID=40&md5=561b42f60079aa08fc39c301cff6efa9},
affiliation={COMAV, Polytechnic University of Valencia, E-46022 Valencia, Spain; Department of Analytical Chemistry, University of Valencia, Burjassot, Spain; Department of Experimental Sciences, Jaumel University, Castellón, Spain; Department of Chemistry, Polytechnic University of Valencia, Valencia, Spain},
abstract={In this study, the major carotenoids (β-carotene and lycopene) present in tomato fruits were analyzed by CEC with a methacrylate ester-based monolithic column. The effects of the porogenic solvent ratio, and the hydrophobicity of bulk monomer employed were examined on carotenoids separations. A fast separation of these analytes was achieved in less than 5.0 min in a mobile phase containing 35% THF, 30% ACN, 30% methanol, and 5% of a 5 mM Tris aqueous buffer, pH 8, with lauryl methacrylate-based monoliths. The CEC method was evaluated in terms of detection limit and reproducibility (retention time, area, and column preparation) with values below 1.6 μg/mL and 7.2%, respectively. The proposed procedure was successfully applied to the determination of both carotenoids in fruits of several tomato-related species and its usefulness to analyze large series of samples for nutritional quality screening trials in tomato breeding programs is demonstrated. To our knowledge, this is the first work that exploits the powerful and user-friendly monolithic technology for quality breeding and germplasm evaluation program purposes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.},
author_keywords={Carotenoids; CEC; Lycopene; Methacrylate ester-based monolithic column; β-Carotene},
keywords={Esters; Fruits; Quality control; Screening, CEC; Fast separation; Lycopenes; Methacrylate ester-based monolithic column; Methacrylate esters; Monolithic columns; Porogenic solvents; Solvent ratios; Tomato fruits; Β-carotene, Carotenoids, beta carotene; buffer; carotenoid; ester derivative; lycopene; methanol; monomer; solvent; trometamol, analytic method; article; capillary electrophoresis; chemical analysis; fruit; germplasm; hydrophobicity; pH; plant breeding; screening; separation technique; tomato},
chemicals_cas={beta carotene, 7235-40-7; lycopene, 502-65-8; methanol, 67-56-1; trometamol, 1185-53-1, 77-86-1},
references={Mangels, A.R., Holden, J.M., Beecher, G.R., Forman, M.R., Lanza, E., (1993) J. Am. Diet. Assoc, 93, pp. 284-296; Britton, G., Hornero-Mendez, D., (1997) Phytochemistry of Fruit and Vegetables, pp. 11-27. , Tomas-Barberan, F. A, Robins, R. J, Eds, Oxford Science Publications, Oxford, UK; Delgado-Vargas, F., Jimenez, A.R., Paredes-Lopez, O., (2000) Crit. Rev. Food Sci. Nutr, 40, pp. 173-289; Beecher, G.R., (1998) Proc. Soc. Exp. Biol. Med, 218, pp. 98-100; Clinton, S.K., (1998) Nutr. Rev, 56, pp. 35-51; Shi, J., Le Maguer, M., (2000) Crit. Rev. Food Sci. Nutr, 40, pp. 1-42; Khachik, F., Beecher, G.R., Goli, M.B., Lusby, W.R., (1991) Pure Appl. Chem, 63, pp. 71-80; Khachik, F., Beecher, G.R., Goli, M.B., Lusby, W.R., (1992) Methods in Enzymology, 213, pp. 347-359. , Packer, L, Ed, Academic Press, New York, Part A, pp; Di Mascio, P., Kaiser, S., Sies, H., (1989) Arch. Biochem. Biophys, 274, pp. 532-538; Ziegler, R.G., (1991) Am. J. Clin. Nutr, 53, pp. 251S-259S; Giovannucci, E., (1999) J. Natl. Cancer Inst, 91, pp. 317-331; Arab, L., Steck, S., (2000) Am. J. Clin. Nutr, 71, pp. 1691S-1695S; Willcox, J.K., Catignani, G.L., Lazarus, S., (2003) Crit. Rev. Food Sci. Nutr, 43, pp. 1-18; Mayne, S.T., (1996) FASEB J, 10, pp. 690-701; Angosto, T., Borja, A., (2001) Biotecnologia de cultivos horticolas, p. 125. , Lozano-Ruiz, R, Cuadrado-Gomez, I. M, Eds, Junta de Andalucia, Almería, Spain; Abushita, A.A., Hebshi, E.A., Daood, H.G., Biacs, P.A., (1997) Food Chem, 60, pp. 207-212; Fernández-Ruiz, V., Sánchez-Mata, M. C., Cámara, M., Torija, M. E., Symposium on Dietary phytochemicals and human health, Salamanca, Spain 2002; Aust, O., Sies, H., Stahl, W., Polidori, M.C., (2001) J. Chromatogr. A, 936, pp. 83-93; Rodriguez-Amaya, D.B., (2001) A Guide to Carotenoid Analysis in Foods, , ILSI Press, Washington; Bushway, R.J., (1985) J. Liq. Chromatogr, 8, pp. 1527-1547; Craft, N.E., Wise, S.A., Soares Jr., J.H., (1992) J. Chromatogr, 589, pp. 171-176; Epler, K.S., Sander, L.C., Zeigler, R.G., Wise, S.A., Craft, N.E., (1992) J. Chromatogr, 595, pp. 89-101; Lesellier, E., Tchapla, A., Krstulovic, A.M., (1993) J. Chromatogr, 645, pp. 29-39; Biacs, P.A., Daood, H.G., (1994) J. Plant Physiol, 143, pp. 520-525; Hart, D.J., Scott, K.J., (1995) Food Chem, 54, pp. 101-111; Thompson, K.A., Marshall, M.R., Sims, C.A., Wei, C.I., (2000) J. Food Sci, 65, pp. 791-795; Sander, L.C., Sharpless, K.E., Pursch, M., (2000) J. Chromatogr. A, 880, pp. 189-202; Lessin, W.J., Catigani, G.L., Schwartz, S.J., (1997) J. Agric. Food Chem, 45, pp. 3728-3732; Lin, C.H., Chen, B.H., (2003) J. Chromatogr. A, 1012, pp. 103-109; Ishida, B.K., Ma, J., Chan, B., (2001) Phytochem. Anal, 12, pp. 194-198; Colón, L.A., Burgos, G., Maloney, T.D., Cintrón, J.M., Rodriguez, R.L., (2000) Electrophoresis, 21, pp. 3965-3993; Hilder, E.F., Svec, F., Fréchet, J.M.J., (2002) Electrophoresis, 23, pp. 3934-3953; Knox, J.H., Grant, I.H., (1991) Chromatographia, 32, pp. 317-328; Smith, N.W., Evans, M.B., (1995) Chromatographia, 41, pp. 197-203; Zhang, M., El Rassi, Z., (2001) Electrophoresis, 22, pp. 2593-2599; Mistry, K., Cortes, H., Meunier, D., Schmidt, C., (2002) Anal. Chem, 74, pp. 617-625; Eeltink, S., Rozing, G.P., Kok, W.T., (2003) Electrophoresis, 24, pp. 3935-3961; Sander, L.C., Pursch, M., Marker, B., Wise, S.A., (1999) Anal. Chem, 71, pp. 3477-3483; Herrero-Martínez, J.M., Eeltink, S., Schoenmakers, P.J., Kok, W.T., Ramis-Ramos, G., (2006) J. Sep. Sci, 29, pp. 660-665; Fujimoto, C., (2002) Anal. Sci, 18, pp. 19-25; Siouffi, A.M., (2003) J. Chromatogr. A, 1000, pp. 801-818; Wu, R., Zou, H., Ye, M., Lei, Z., Ni, J., (2001) Anal. Chem, 73, pp. 4918-4923; Gusev, I., Huang, X., Horváth, C., (1999) J. Chromatogr. A, 855, pp. 273-290; Svec, F., Fréchet, J.M.J., (1996) Science, 273, pp. 205-211; Peters, E.C., Petro, M., Svec, F., Fréchet, J.M.J., (1998) Anal. Chem, 70, pp. 2288-2295; Jiang, T., Jiskra, J., Claessens, H.A., Cramers, C.A., (2001) J. Chromatogr. A, 923, pp. 215-227; Que, A.H., Konse, T., Baker, A.G., Novotny, M.V., (2000) Anal. Chem, 72, pp. 2703-2710; Bandilla, D., Skinner, C.D., (2003) J. Chromatogr. A, 1004, pp. 167-179; Mistry, K., Krull, I., Grinberg, N., (2003) Electrophoresis, 24, pp. 1753-1763; Kvasnickova, L., Glatz, Z., Sterbova, H., Kahle, V., (2001) J. Chromatogr. A, 916, pp. 265-271; Huang, H.Y., Chiu, C.W., Huang, I.Y., Yeh, J.M., (2004) Electrophoresis, 25, pp. 3237-3246; Peters, E.C., Petro, M., Svec, F., Fréchet, J.M.J., (1997) Anal. Chem, 69, pp. 3646-3649; Viklund, C., Svec, F., Fréchet, J.M.J., Irgum, I., (1996) Chem. Mater, 8, pp. 744-750; Eeltink, B., Herrero-Martínez, J.M., Rozing, G.P., Schoenmakers, P.J., Kok, W.T., (2005) Anal. Chem, 77, pp. 7342-7347; Davey, M.W., Keulemans, J., Swennen, R., (2006) J. Chromatogr. A, 1136, pp. 176-184; Nguyen, M.L., Schwartz, S.J., (1999) Food Technol, 53, pp. 38-45; Zscheile, F.P., Porter, J.W., (1947) Anal. Chem, 19, pp. 47-51; Fish, W.W., Perkins-Veazie, P., Collins, J.K., (2002) J. Food Compost. Anal, 15, pp. 309-317; Roselló, S., Adalid, A.M., Cebolla-Cornejo, J., Nuez, F., (2005) Actas Portuguesas de Horticultura, 8, pp. 429-435; Adalid, A.M., Roselló, S., Cebolla-Cornejo, J., Nuez, F., (2007) Acta Physiol. Plant, , in press; Davis, A.R., Fish, W.W., Perkins-Veazie, P., (2003) Postharvest Biol. Technol, 28, pp. 425-430},
correspondence_address1={Nuez, F.; COMAV, , E-46022 Valencia, Spain; email: fnuez@btc.upv.es},
publisher={Wiley-VCH Verlag},
issn={01730835},
coden={ELCTD},
pubmed_id={17960534},
language={English},
abbrev_source_title={Electrophoresis},
document_type={Article},
source={Scopus},
}
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{"_id":"P7DnTsCLBLdMRrJzd","bibbaseid":"adalid-herreromartnez-rosell-maquieira-nuez-fastdeterminationofprominentcarotenoidsintomatofruitsbycecusingmethacrylateesterbasedmonolithiccolumns-2007","author_short":["Adalid, A.","Herrero-Martínez, J.","Roselló, S.","Maquieira, A.","Nuez, F."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Adalid"],"firstnames":["A.M."],"suffixes":[]},{"propositions":[],"lastnames":["Herrero-Martínez"],"firstnames":["J.M."],"suffixes":[]},{"propositions":[],"lastnames":["Roselló"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Maquieira"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Nuez"],"firstnames":["F."],"suffixes":[]}],"title":"Fast determination of prominent carotenoids in tomato fruits by CEC using methacrylate ester-based monolithic columns","journal":"Electrophoresis","year":"2007","volume":"28","number":"22","pages":"4120-4127","doi":"10.1002/elps.200600775","note":"cited By 14","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36549071138&doi=10.1002%2felps.200600775&partnerID=40&md5=561b42f60079aa08fc39c301cff6efa9","affiliation":"COMAV, Polytechnic University of Valencia, E-46022 Valencia, Spain; Department of Analytical Chemistry, University of Valencia, Burjassot, Spain; Department of Experimental Sciences, Jaumel University, Castellón, Spain; Department of Chemistry, Polytechnic University of Valencia, Valencia, Spain","abstract":"In this study, the major carotenoids (β-carotene and lycopene) present in tomato fruits were analyzed by CEC with a methacrylate ester-based monolithic column. The effects of the porogenic solvent ratio, and the hydrophobicity of bulk monomer employed were examined on carotenoids separations. A fast separation of these analytes was achieved in less than 5.0 min in a mobile phase containing 35% THF, 30% ACN, 30% methanol, and 5% of a 5 mM Tris aqueous buffer, pH 8, with lauryl methacrylate-based monoliths. The CEC method was evaluated in terms of detection limit and reproducibility (retention time, area, and column preparation) with values below 1.6 μg/mL and 7.2%, respectively. The proposed procedure was successfully applied to the determination of both carotenoids in fruits of several tomato-related species and its usefulness to analyze large series of samples for nutritional quality screening trials in tomato breeding programs is demonstrated. To our knowledge, this is the first work that exploits the powerful and user-friendly monolithic technology for quality breeding and germplasm evaluation program purposes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.","author_keywords":"Carotenoids; CEC; Lycopene; Methacrylate ester-based monolithic column; β-Carotene","keywords":"Esters; Fruits; Quality control; Screening, CEC; Fast separation; Lycopenes; Methacrylate ester-based monolithic column; Methacrylate esters; Monolithic columns; Porogenic solvents; Solvent ratios; Tomato fruits; Β-carotene, Carotenoids, beta carotene; buffer; carotenoid; ester derivative; lycopene; methanol; monomer; solvent; trometamol, analytic method; article; capillary electrophoresis; chemical analysis; fruit; germplasm; hydrophobicity; pH; plant breeding; screening; separation technique; tomato","chemicals_cas":"beta carotene, 7235-40-7; lycopene, 502-65-8; methanol, 67-56-1; trometamol, 1185-53-1, 77-86-1","references":"Mangels, A.R., Holden, J.M., Beecher, G.R., Forman, M.R., Lanza, E., (1993) J. Am. Diet. Assoc, 93, pp. 284-296; Britton, G., Hornero-Mendez, D., (1997) Phytochemistry of Fruit and Vegetables, pp. 11-27. , Tomas-Barberan, F. A, Robins, R. J, Eds, Oxford Science Publications, Oxford, UK; Delgado-Vargas, F., Jimenez, A.R., Paredes-Lopez, O., (2000) Crit. Rev. Food Sci. Nutr, 40, pp. 173-289; Beecher, G.R., (1998) Proc. Soc. Exp. Biol. Med, 218, pp. 98-100; Clinton, S.K., (1998) Nutr. Rev, 56, pp. 35-51; Shi, J., Le Maguer, M., (2000) Crit. Rev. Food Sci. Nutr, 40, pp. 1-42; Khachik, F., Beecher, G.R., Goli, M.B., Lusby, W.R., (1991) Pure Appl. Chem, 63, pp. 71-80; Khachik, F., Beecher, G.R., Goli, M.B., Lusby, W.R., (1992) Methods in Enzymology, 213, pp. 347-359. , Packer, L, Ed, Academic Press, New York, Part A, pp; Di Mascio, P., Kaiser, S., Sies, H., (1989) Arch. Biochem. Biophys, 274, pp. 532-538; Ziegler, R.G., (1991) Am. J. Clin. Nutr, 53, pp. 251S-259S; Giovannucci, E., (1999) J. Natl. Cancer Inst, 91, pp. 317-331; Arab, L., Steck, S., (2000) Am. J. Clin. Nutr, 71, pp. 1691S-1695S; Willcox, J.K., Catignani, G.L., Lazarus, S., (2003) Crit. Rev. Food Sci. Nutr, 43, pp. 1-18; Mayne, S.T., (1996) FASEB J, 10, pp. 690-701; Angosto, T., Borja, A., (2001) Biotecnologia de cultivos horticolas, p. 125. , Lozano-Ruiz, R, Cuadrado-Gomez, I. M, Eds, Junta de Andalucia, Almería, Spain; Abushita, A.A., Hebshi, E.A., Daood, H.G., Biacs, P.A., (1997) Food Chem, 60, pp. 207-212; Fernández-Ruiz, V., Sánchez-Mata, M. C., Cámara, M., Torija, M. E., Symposium on Dietary phytochemicals and human health, Salamanca, Spain 2002; Aust, O., Sies, H., Stahl, W., Polidori, M.C., (2001) J. Chromatogr. A, 936, pp. 83-93; Rodriguez-Amaya, D.B., (2001) A Guide to Carotenoid Analysis in Foods, , ILSI Press, Washington; Bushway, R.J., (1985) J. Liq. Chromatogr, 8, pp. 1527-1547; Craft, N.E., Wise, S.A., Soares Jr., J.H., (1992) J. Chromatogr, 589, pp. 171-176; Epler, K.S., Sander, L.C., Zeigler, R.G., Wise, S.A., Craft, N.E., (1992) J. Chromatogr, 595, pp. 89-101; Lesellier, E., Tchapla, A., Krstulovic, A.M., (1993) J. Chromatogr, 645, pp. 29-39; Biacs, P.A., Daood, H.G., (1994) J. Plant Physiol, 143, pp. 520-525; Hart, D.J., Scott, K.J., (1995) Food Chem, 54, pp. 101-111; Thompson, K.A., Marshall, M.R., Sims, C.A., Wei, C.I., (2000) J. Food Sci, 65, pp. 791-795; Sander, L.C., Sharpless, K.E., Pursch, M., (2000) J. Chromatogr. A, 880, pp. 189-202; Lessin, W.J., Catigani, G.L., Schwartz, S.J., (1997) J. Agric. Food Chem, 45, pp. 3728-3732; Lin, C.H., Chen, B.H., (2003) J. Chromatogr. A, 1012, pp. 103-109; Ishida, B.K., Ma, J., Chan, B., (2001) Phytochem. Anal, 12, pp. 194-198; Colón, L.A., Burgos, G., Maloney, T.D., Cintrón, J.M., Rodriguez, R.L., (2000) Electrophoresis, 21, pp. 3965-3993; Hilder, E.F., Svec, F., Fréchet, J.M.J., (2002) Electrophoresis, 23, pp. 3934-3953; Knox, J.H., Grant, I.H., (1991) Chromatographia, 32, pp. 317-328; Smith, N.W., Evans, M.B., (1995) Chromatographia, 41, pp. 197-203; Zhang, M., El Rassi, Z., (2001) Electrophoresis, 22, pp. 2593-2599; Mistry, K., Cortes, H., Meunier, D., Schmidt, C., (2002) Anal. Chem, 74, pp. 617-625; Eeltink, S., Rozing, G.P., Kok, W.T., (2003) Electrophoresis, 24, pp. 3935-3961; Sander, L.C., Pursch, M., Marker, B., Wise, S.A., (1999) Anal. Chem, 71, pp. 3477-3483; Herrero-Martínez, J.M., Eeltink, S., Schoenmakers, P.J., Kok, W.T., Ramis-Ramos, G., (2006) J. Sep. Sci, 29, pp. 660-665; Fujimoto, C., (2002) Anal. Sci, 18, pp. 19-25; Siouffi, A.M., (2003) J. Chromatogr. A, 1000, pp. 801-818; Wu, R., Zou, H., Ye, M., Lei, Z., Ni, J., (2001) Anal. Chem, 73, pp. 4918-4923; Gusev, I., Huang, X., Horváth, C., (1999) J. Chromatogr. A, 855, pp. 273-290; Svec, F., Fréchet, J.M.J., (1996) Science, 273, pp. 205-211; Peters, E.C., Petro, M., Svec, F., Fréchet, J.M.J., (1998) Anal. Chem, 70, pp. 2288-2295; Jiang, T., Jiskra, J., Claessens, H.A., Cramers, C.A., (2001) J. Chromatogr. A, 923, pp. 215-227; Que, A.H., Konse, T., Baker, A.G., Novotny, M.V., (2000) Anal. Chem, 72, pp. 2703-2710; Bandilla, D., Skinner, C.D., (2003) J. Chromatogr. A, 1004, pp. 167-179; Mistry, K., Krull, I., Grinberg, N., (2003) Electrophoresis, 24, pp. 1753-1763; Kvasnickova, L., Glatz, Z., Sterbova, H., Kahle, V., (2001) J. Chromatogr. A, 916, pp. 265-271; Huang, H.Y., Chiu, C.W., Huang, I.Y., Yeh, J.M., (2004) Electrophoresis, 25, pp. 3237-3246; Peters, E.C., Petro, M., Svec, F., Fréchet, J.M.J., (1997) Anal. Chem, 69, pp. 3646-3649; Viklund, C., Svec, F., Fréchet, J.M.J., Irgum, I., (1996) Chem. Mater, 8, pp. 744-750; Eeltink, B., Herrero-Martínez, J.M., Rozing, G.P., Schoenmakers, P.J., Kok, W.T., (2005) Anal. Chem, 77, pp. 7342-7347; Davey, M.W., Keulemans, J., Swennen, R., (2006) J. Chromatogr. A, 1136, pp. 176-184; Nguyen, M.L., Schwartz, S.J., (1999) Food Technol, 53, pp. 38-45; Zscheile, F.P., Porter, J.W., (1947) Anal. Chem, 19, pp. 47-51; Fish, W.W., Perkins-Veazie, P., Collins, J.K., (2002) J. Food Compost. Anal, 15, pp. 309-317; Roselló, S., Adalid, A.M., Cebolla-Cornejo, J., Nuez, F., (2005) Actas Portuguesas de Horticultura, 8, pp. 429-435; Adalid, A.M., Roselló, S., Cebolla-Cornejo, J., Nuez, F., (2007) Acta Physiol. Plant, , in press; Davis, A.R., Fish, W.W., Perkins-Veazie, P., (2003) Postharvest Biol. Technol, 28, pp. 425-430","correspondence_address1":"Nuez, F.; COMAV, , E-46022 Valencia, Spain; email: fnuez@btc.upv.es","publisher":"Wiley-VCH Verlag","issn":"01730835","coden":"ELCTD","pubmed_id":"17960534","language":"English","abbrev_source_title":"Electrophoresis","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Adalid20074120,\nauthor={Adalid, A.M. and Herrero-Martínez, J.M. and Roselló, S. and Maquieira, A. and Nuez, F.},\ntitle={Fast determination of prominent carotenoids in tomato fruits by CEC using methacrylate ester-based monolithic columns},\njournal={Electrophoresis},\nyear={2007},\nvolume={28},\nnumber={22},\npages={4120-4127},\ndoi={10.1002/elps.200600775},\nnote={cited By 14},\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-36549071138&doi=10.1002%2felps.200600775&partnerID=40&md5=561b42f60079aa08fc39c301cff6efa9},\naffiliation={COMAV, Polytechnic University of Valencia, E-46022 Valencia, Spain; Department of Analytical Chemistry, University of Valencia, Burjassot, Spain; Department of Experimental Sciences, Jaumel University, Castellón, Spain; Department of Chemistry, Polytechnic University of Valencia, Valencia, Spain},\nabstract={In this study, the major carotenoids (β-carotene and lycopene) present in tomato fruits were analyzed by CEC with a methacrylate ester-based monolithic column. The effects of the porogenic solvent ratio, and the hydrophobicity of bulk monomer employed were examined on carotenoids separations. A fast separation of these analytes was achieved in less than 5.0 min in a mobile phase containing 35% THF, 30% ACN, 30% methanol, and 5% of a 5 mM Tris aqueous buffer, pH 8, with lauryl methacrylate-based monoliths. The CEC method was evaluated in terms of detection limit and reproducibility (retention time, area, and column preparation) with values below 1.6 μg/mL and 7.2%, respectively. The proposed procedure was successfully applied to the determination of both carotenoids in fruits of several tomato-related species and its usefulness to analyze large series of samples for nutritional quality screening trials in tomato breeding programs is demonstrated. To our knowledge, this is the first work that exploits the powerful and user-friendly monolithic technology for quality breeding and germplasm evaluation program purposes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.},\nauthor_keywords={Carotenoids; CEC; Lycopene; Methacrylate ester-based monolithic column; β-Carotene},\nkeywords={Esters; Fruits; Quality control; Screening, CEC; Fast separation; Lycopenes; Methacrylate ester-based monolithic column; Methacrylate esters; Monolithic columns; Porogenic solvents; Solvent ratios; Tomato fruits; Β-carotene, Carotenoids, beta carotene; buffer; carotenoid; ester derivative; lycopene; methanol; monomer; solvent; trometamol, analytic method; article; capillary electrophoresis; chemical analysis; fruit; germplasm; hydrophobicity; pH; plant breeding; screening; separation technique; tomato},\nchemicals_cas={beta carotene, 7235-40-7; lycopene, 502-65-8; methanol, 67-56-1; trometamol, 1185-53-1, 77-86-1},\nreferences={Mangels, A.R., Holden, J.M., Beecher, G.R., Forman, M.R., Lanza, E., (1993) J. Am. Diet. Assoc, 93, pp. 284-296; Britton, G., Hornero-Mendez, D., (1997) Phytochemistry of Fruit and Vegetables, pp. 11-27. , Tomas-Barberan, F. 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Technol, 28, pp. 425-430},\ncorrespondence_address1={Nuez, F.; COMAV, , E-46022 Valencia, Spain; email: fnuez@btc.upv.es},\npublisher={Wiley-VCH Verlag},\nissn={01730835},\ncoden={ELCTD},\npubmed_id={17960534},\nlanguage={English},\nabbrev_source_title={Electrophoresis},\ndocument_type={Article},\nsource={Scopus},\n}\n\n","author_short":["Adalid, A.","Herrero-Martínez, J.","Roselló, S.","Maquieira, A.","Nuez, F."],"key":"Adalid20074120","id":"Adalid20074120","bibbaseid":"adalid-herreromartnez-rosell-maquieira-nuez-fastdeterminationofprominentcarotenoidsintomatofruitsbycecusingmethacrylateesterbasedmonolithiccolumns-2007","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-36549071138&doi=10.1002%2felps.200600775&partnerID=40&md5=561b42f60079aa08fc39c301cff6efa9"},"keyword":["Esters; Fruits; Quality control; Screening","CEC; Fast separation; Lycopenes; Methacrylate ester-based monolithic column; Methacrylate esters; Monolithic columns; Porogenic solvents; Solvent ratios; Tomato fruits; Β-carotene","Carotenoids","beta carotene; buffer; carotenoid; ester derivative; lycopene; methanol; monomer; solvent; trometamol","analytic method; article; capillary electrophoresis; chemical analysis; fruit; germplasm; hydrophobicity; pH; plant breeding; screening; separation technique; tomato"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://www.clecem.es/wp-content/uploads/2024/01/scopus.bib","dataSources":["z88YG74vvXjjYpRcn"],"keywords":["esters; fruits; quality control; screening","cec; fast separation; lycopenes; methacrylate ester-based monolithic column; methacrylate esters; monolithic columns; porogenic solvents; solvent ratios; tomato fruits; β-carotene","carotenoids","beta carotene; buffer; carotenoid; ester derivative; lycopene; methanol; monomer; solvent; trometamol","analytic method; article; capillary electrophoresis; chemical analysis; fruit; germplasm; hydrophobicity; ph; plant breeding; screening; separation technique; tomato"],"search_terms":["fast","determination","prominent","carotenoids","tomato","fruits","cec","using","methacrylate","ester","based","monolithic","columns","adalid","herrero-martínez","roselló","maquieira","nuez"],"title":"Fast determination of prominent carotenoids in tomato fruits by CEC using methacrylate ester-based monolithic columns","year":2007}