Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products. Adams, J. D., Sander, K. B., Criddle, C. S., Arkin, A. P., & Clark, D. S. Microbial Cell Factories, 22(1):69, April, 2023. ![Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Intracellular biomacromolecules, such as industrial enzymes and biopolymers, represent an important class of bio-derived products obtained from bacterial hosts. A common key step in the downstream separation of these biomolecules is lysis of the bacterial cell wall to effect release of cytoplasmic contents. Cell lysis is typically achieved either through mechanical disruption or reagent-based methods, which introduce issues of energy demand, material needs, high costs, and scaling problems. Osmolysis, a cell lysis method that relies on hypoosmotic downshock upon resuspension of cells in distilled water, has been applied for bioseparation of intracellular products from extreme halophiles and mammalian cells. However, most industrial bacterial strains are non-halotolerant and relatively resistant to hypoosmotic cell lysis.
@article{adams_engineering_2023,
title = {Engineering osmolysis susceptibility in {Cupriavidus} necator and {Escherichia} coli for recovery of intracellular products},
volume = {22},
issn = {1475-2859},
url = {https://doi.org/10.1186/s12934-023-02064-8},
doi = {10.1186/s12934-023-02064-8},
abstract = {Intracellular biomacromolecules, such as industrial enzymes and biopolymers, represent an important class of bio-derived products obtained from bacterial hosts. A common key step in the downstream separation of these biomolecules is lysis of the bacterial cell wall to effect release of cytoplasmic contents. Cell lysis is typically achieved either through mechanical disruption or reagent-based methods, which introduce issues of energy demand, material needs, high costs, and scaling problems. Osmolysis, a cell lysis method that relies on hypoosmotic downshock upon resuspension of cells in distilled water, has been applied for bioseparation of intracellular products from extreme halophiles and mammalian cells. However, most industrial bacterial strains are non-halotolerant and relatively resistant to hypoosmotic cell lysis.},
language = {en},
number = {1},
urldate = {2024-02-29},
journal = {Microbial Cell Factories},
author = {Adams, Jeremy David and Sander, Kyle B. and Criddle, Craig S. and Arkin, Adam P. and Clark, Douglas S.},
month = apr,
year = {2023},
keywords = {Precourt, SDSS, SOE, Sustainability},
pages = {69},
}
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