PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer. Agarwal, V., Sardo, M., Scholz, I., Böckmann, A., Ernst, M., & Meier, B. H Journal of biomolecular NMR, 56(4):365–377, August, 2013. ![PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex In this article, we describe third-spin assisted heteronuclear recoupling experiments, which play an increasingly important role in measuring long-range heteronuclear couplings, in particular (15)N-(13)C, in proteins. In the proton-assisted insensitive nuclei cross polarization (PAIN-CP) experiment (de Paëpe et al. in J Chem Phys 134:095101, 2011), heteronuclear polarization transfer is always accompanied by homonuclear transfer of the proton-assisted recoupling (PAR) type. We present a phase-alternating experiment that promotes heteronuclear (e.g. (15)N → (13)C) polarization transfer while simultaneously minimizing homonuclear (e.g.(13)C → (13)C) transfer (PAIN without PAR). This minimization of homonuclear polarization transfer is based on the principle of the resonant second-order transfer (RESORT) recoupling scheme where the passive proton spins are irradiated by a phase-alternating sequence and the modulation frequency is matched to an integer multiple of the spinning frequency. The similarities and differences between the PAIN-CP and this het-RESORT experiment are discussed here.
@article{Agarwal2013,
title = {{PAIN} with and without {PAR}: variants for third-spin assisted heteronuclear polarization transfer.},
volume = {56},
issn = {1573-5001},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23807391},
doi = {10.1007/s10858-013-9756-4},
abstract = {In this article, we describe third-spin assisted heteronuclear recoupling experiments, which play an increasingly important role in measuring long-range heteronuclear couplings, in particular (15)N-(13)C, in proteins. In the proton-assisted insensitive nuclei cross polarization (PAIN-CP) experiment (de Paëpe et al. in J Chem Phys 134:095101, 2011), heteronuclear polarization transfer is always accompanied by homonuclear transfer of the proton-assisted recoupling (PAR) type. We present a phase-alternating experiment that promotes heteronuclear (e.g. (15)N → (13)C) polarization transfer while simultaneously minimizing homonuclear (e.g.(13)C → (13)C) transfer (PAIN without PAR). This minimization of homonuclear polarization transfer is based on the principle of the resonant second-order transfer (RESORT) recoupling scheme where the passive proton spins are irradiated by a phase-alternating sequence and the modulation frequency is matched to an integer multiple of the spinning frequency. The similarities and differences between the PAIN-CP and this het-RESORT experiment are discussed here.},
number = {4},
journal = {Journal of biomolecular NMR},
author = {Agarwal, Vipin and Sardo, Mariana and Scholz, Ingo and Böckmann, Anja and Ernst, Matthias and Meier, Beat H},
month = aug,
year = {2013},
pmid = {23807391},
keywords = {\#nosource, Biomolecular, Computer Simulation, Crystallization, Nuclear Magnetic Resonance, Protons, Spin Labels, Ubiquitin, Ubiquitin: chemistry},
pages = {365--377},
}
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