Electrochemiluminescent immunosensor for detection of carcinoembryonic antigen using luminol-coated silver nanoparticles. Akbari Nakhjavani, S., Khalilzadeh, B., Afsharan, H., Hosseini, N., Ghahremani, M. H., Carrara, S., Tasoglu, S., & Omidi, Y. Mikrochim. Acta, 190(2):77, January, 2023. ![Electrochemiluminescent immunosensor for detection of carcinoembryonic antigen using luminol-coated silver nanoparticles [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 1 download Recently, electrochemiluminescent (ECL) immunosensors have received much attention in the field of biomarker detection. Here, a highly enhanced ECL immunosensing platform was designed for ultrasensitive detection of carcinoembryonic antigen (CEA). The surface of the glassy carbon electrode was enhanced by applying functional nanostructures such as thiolated graphene oxide (S-GO) and streptavidin-coated gold nanoparticles (SA-AuNPs). The selectivity and sensitivity of the designed immunosensor were improved by entrapping CEA biomolecules using a sandwich approach. Luminol/silver nanoparticles (Lu-SNPs) were applied as the main core of the signaling probe, which were then coated with streptavidin to provide overloading of the secondary antibody. The highly ECL signal enhancement was obtained due to the presence of horseradish peroxidase (HRP) in the signaling probe, in which the presence of H2O2 further amplified the intensity of the signals. The engineered immunosensor presented excellent sensitivity for CEA detection, with limit of detection (LOD) and linear detection range (LDR) values of 58 fg mL-1 and 0.1 pg mL-1 to 5 pg mL-1 (R2 = 0.9944), respectively. Besides its sensitivity, the fabricated ECL immunosensor presented outstanding selectivity for the detection of CEA in the presence of various similar agents. Additionally, the developed immunosensor showed an appropriate repeatability (RSD 3.8%) and proper stability (2 weeks). Having indicated a robust performance in the real human serum with stated LOD and LDR, the engineered immunosensor can be considered for the detection and monitoring of CEA in the clinic.
@article{akbari_nakhjavani_electrochemiluminescent_2023,
title = {Electrochemiluminescent immunosensor for detection of carcinoembryonic antigen using luminol-coated silver nanoparticles},
volume = {190},
issn = {0026-3672},
url = {http://dx.doi.org/10.1007/s00604-023-05656-8},
doi = {10.1007/s00604-023-05656-8},
abstract = {Recently, electrochemiluminescent (ECL) immunosensors have received much
attention in the field of biomarker detection. Here, a highly enhanced ECL
immunosensing platform was designed for ultrasensitive detection of
carcinoembryonic antigen (CEA). The surface of the glassy carbon electrode
was enhanced by applying functional nanostructures such as thiolated
graphene oxide (S-GO) and streptavidin-coated gold nanoparticles
(SA-AuNPs). The selectivity and sensitivity of the designed immunosensor
were improved by entrapping CEA biomolecules using a sandwich approach.
Luminol/silver nanoparticles (Lu-SNPs) were applied as the main core of
the signaling probe, which were then coated with streptavidin to provide
overloading of the secondary antibody. The highly ECL signal enhancement
was obtained due to the presence of horseradish peroxidase (HRP) in the
signaling probe, in which the presence of H2O2 further amplified the
intensity of the signals. The engineered immunosensor presented excellent
sensitivity for CEA detection, with limit of detection (LOD) and linear
detection range (LDR) values of 58 fg mL-1 and 0.1 pg mL-1 to 5 pg mL-1
(R2 = 0.9944), respectively. Besides its sensitivity, the fabricated ECL
immunosensor presented outstanding selectivity for the detection of CEA in
the presence of various similar agents. Additionally, the developed
immunosensor showed an appropriate repeatability (RSD 3.8\%) and proper
stability (2 weeks). Having indicated a robust performance in the real
human serum with stated LOD and LDR, the engineered immunosensor can be
considered for the detection and monitoring of CEA in the clinic.},
number = {2},
journal = {Mikrochim. Acta},
author = {Akbari Nakhjavani, Sattar and Khalilzadeh, Balal and Afsharan, Hadi and Hosseini, Nashmin and Ghahremani, Mohammad Hossein and Carrara, Sandro and Tasoglu, Savas and Omidi, Yadollah},
month = jan,
year = {2023},
keywords = {Bio-nanocomposite, Biosensor, Breast cancer, Electrochemiluminescence, Immunosensor, Savas Scholar},
pages = {77},
}
Downloads: 1
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The surface of the glassy carbon electrode was enhanced by applying functional nanostructures such as thiolated graphene oxide (S-GO) and streptavidin-coated gold nanoparticles (SA-AuNPs). The selectivity and sensitivity of the designed immunosensor were improved by entrapping CEA biomolecules using a sandwich approach. Luminol/silver nanoparticles (Lu-SNPs) were applied as the main core of the signaling probe, which were then coated with streptavidin to provide overloading of the secondary antibody. The highly ECL signal enhancement was obtained due to the presence of horseradish peroxidase (HRP) in the signaling probe, in which the presence of H2O2 further amplified the intensity of the signals. The engineered immunosensor presented excellent sensitivity for CEA detection, with limit of detection (LOD) and linear detection range (LDR) values of 58 fg mL-1 and 0.1 pg mL-1 to 5 pg mL-1 (R2 = 0.9944), respectively. Besides its sensitivity, the fabricated ECL immunosensor presented outstanding selectivity for the detection of CEA in the presence of various similar agents. Additionally, the developed immunosensor showed an appropriate repeatability (RSD 3.8%) and proper stability (2 weeks). Having indicated a robust performance in the real human serum with stated LOD and LDR, the engineered immunosensor can be considered for the detection and monitoring of CEA in the clinic.","number":"2","journal":"Mikrochim. 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