Simultaneous Quantification of Cells and Nanomaterials by Inductive-Coupled Plasma Techniques. Albanese, A., Tsoi, K. M., & Chan, W. C. W. J Lab Autom., 18(1):99–104, February, 2013. Publisher: SAGE Publications IncPaper Paper doi abstract bibtex We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.
@article{albanese_simultaneous_2013,
title = {Simultaneous {Quantification} of {Cells} and {Nanomaterials} by {Inductive}-{Coupled} {Plasma} {Techniques}},
volume = {18},
issn = {2211-0682},
url = {https://doi.org/10.1177/2211068212457039},
doi = {10.1177/2211068212457039},
abstract = {We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.},
language = {en},
number = {1},
urldate = {2021-11-06},
journal = {J Lab Autom.},
author = {Albanese, Alexandre and Tsoi, Kim M. and Chan, Warren C. W.},
month = feb,
year = {2013},
note = {Publisher: SAGE Publications Inc},
keywords = {cell quantification, cell uptake, cellular elemental content, gold nano-urchins, gold nanoparticles, ICP-AES},
pages = {99--104},
file = {SAGE PDF Full Text:files/2087/Albanese et al. - 2013 - Simultaneous Quantification of Cells and Nanomater.pdf:application/pdf},
url_Paper = {https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/2211068212457039.pdf}
}
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