@article{
 title = {Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans},
 type = {article},
 year = {2018},
 identifiers = {[object Object]},
 pages = {39},
 volume = {1047612},
 id = {c1311521-862e-3538-af50-3051156340ba},
 created = {2019-12-17T17:09:18.166Z},
 file_attached = {true},
 profile_id = {9a94bc2c-179e-361a-8ebd-41a00966522f},
 last_modified = {2019-12-17T17:09:35.706Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 private_publication = {false},
 abstract = {Resistance to various anthelmintic drugs is reported in many animals and
can become a severe problem for human and animal health. In this study,
Photogem (R) and three curcuminoids compounds (curcumin,
demethoxycurcumin, bisdemethoxycurcumin) were used as photosensitizers
in the photodynamic inactivation (PDI) in the helminth model
Caenorhabditis elegans to investigate the ability of this procedure to
worm life cycle. Initially, the presence and location of the
photosensitizers in the worm's body were verified by fluorescence
confocal microscopy. Curcumin was deposited in the digestive tract and
Photogem (R) along the body of the animal in the incubation time of 12
hours with the photosensitizer. Subsequently, a PDI procedure using a
LED device was performed to illuminate the worms treated with the
photosensitizers. The worms were observed by optical microscopy until 48
hours after the PDI to verify the changes in motility, the presence of
eggs and larvae and the number of live worms. Curcuminoids tested
separately and in combination and two light doses of 30 J/m(2) no
changes were observed in the life cycle of the worm at concentrations of
2 mM and 1 mM. However, in treatment with Photogem (R) and a light dose
of 100 J/m(2) a reduction in motility and reproduction of the worm with
0.2 mg/mL was observed after 6 hours of exposure, in addition to the
death of most worms at concentrations of 6, 4, and 2 mg/mL. We suggest,
therefore, that photodynamic inactivation with Photogem (R) may present
an anthelmintic effect against C. elegans, but there is a need for
studies on helminths with parasitic activity.},
 bibtype = {article},
 author = {Albuquerque, Yulli and Anibal, Fernanda F. and de Souza, Clovis W. O. and de Oliveira, Kleber T. and Inada, Natalia M. and Afonso, Ana and Souza, Larissa M. and Pratavieira, Sebastião and Bagnato, Vanderlei S.},
 number = {February}
}

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In this study,\nPhotogem (R) and three curcuminoids compounds (curcumin,\ndemethoxycurcumin, bisdemethoxycurcumin) were used as photosensitizers\nin the photodynamic inactivation (PDI) in the helminth model\nCaenorhabditis elegans to investigate the ability of this procedure to\nworm life cycle. Initially, the presence and location of the\nphotosensitizers in the worm's body were verified by fluorescence\nconfocal microscopy. Curcumin was deposited in the digestive tract and\nPhotogem (R) along the body of the animal in the incubation time of 12\nhours with the photosensitizer. Subsequently, a PDI procedure using a\nLED device was performed to illuminate the worms treated with the\nphotosensitizers. The worms were observed by optical microscopy until 48\nhours after the PDI to verify the changes in motility, the presence of\neggs and larvae and the number of live worms. Curcuminoids tested\nseparately and in combination and two light doses of 30 J/m(2) no\nchanges were observed in the life cycle of the worm at concentrations of\n2 mM and 1 mM. However, in treatment with Photogem (R) and a light dose\nof 100 J/m(2) a reduction in motility and reproduction of the worm with\n0.2 mg/mL was observed after 6 hours of exposure, in addition to the\ndeath of most worms at concentrations of 6, 4, and 2 mg/mL. We suggest,\ntherefore, that photodynamic inactivation with Photogem (R) may present\nan anthelmintic effect against C. elegans, but there is a need for\nstudies on helminths with parasitic activity.","bibtype":"article","author":"Albuquerque, Yulli and Anibal, Fernanda F. and de Souza, Clovis W. O. and de Oliveira, Kleber T. and Inada, Natalia M. and Afonso, Ana and Souza, Larissa M. and Pratavieira, Sebastião and Bagnato, Vanderlei S.","number":"February","bibtex":"@article{\n title = {Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans},\n type = {article},\n year = {2018},\n identifiers = {[object Object]},\n pages = {39},\n volume = {1047612},\n id = {c1311521-862e-3538-af50-3051156340ba},\n created = {2019-12-17T17:09:18.166Z},\n file_attached = {true},\n profile_id = {9a94bc2c-179e-361a-8ebd-41a00966522f},\n last_modified = {2019-12-17T17:09:35.706Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Resistance to various anthelmintic drugs is reported in many animals and\ncan become a severe problem for human and animal health. In this study,\nPhotogem (R) and three curcuminoids compounds (curcumin,\ndemethoxycurcumin, bisdemethoxycurcumin) were used as photosensitizers\nin the photodynamic inactivation (PDI) in the helminth model\nCaenorhabditis elegans to investigate the ability of this procedure to\nworm life cycle. Initially, the presence and location of the\nphotosensitizers in the worm's body were verified by fluorescence\nconfocal microscopy. Curcumin was deposited in the digestive tract and\nPhotogem (R) along the body of the animal in the incubation time of 12\nhours with the photosensitizer. Subsequently, a PDI procedure using a\nLED device was performed to illuminate the worms treated with the\nphotosensitizers. The worms were observed by optical microscopy until 48\nhours after the PDI to verify the changes in motility, the presence of\neggs and larvae and the number of live worms. Curcuminoids tested\nseparately and in combination and two light doses of 30 J/m(2) no\nchanges were observed in the life cycle of the worm at concentrations of\n2 mM and 1 mM. However, in treatment with Photogem (R) and a light dose\nof 100 J/m(2) a reduction in motility and reproduction of the worm with\n0.2 mg/mL was observed after 6 hours of exposure, in addition to the\ndeath of most worms at concentrations of 6, 4, and 2 mg/mL. We suggest,\ntherefore, that photodynamic inactivation with Photogem (R) may present\nan anthelmintic effect against C. elegans, but there is a need for\nstudies on helminths with parasitic activity.},\n bibtype = {article},\n author = {Albuquerque, Yulli and Anibal, Fernanda F. and de Souza, Clovis W. O. and de Oliveira, Kleber T. and Inada, Natalia M. and Afonso, Ana and Souza, Larissa M. and Pratavieira, Sebastião and Bagnato, Vanderlei S.},\n number = {February}\n}","author_short":["Albuquerque, Y.","Anibal, F., F.","de Souza, C., W., O.","de Oliveira, K., T.","Inada, N., M.","Afonso, A.","Souza, L., M.","Pratavieira, S.","Bagnato, V., S."],"urls":{"Paper":"https://bibbase.org/service/mendeley/9a94bc2c-179e-361a-8ebd-41a00966522f/file/6a52fba5-b819-56cb-4f0a-d2b3a7892970/Resumo_Completo___Photodynamic_inactivation_using_curcuminoids_and_Photogem_on_caenorhabditis_elegans.pdf.pdf"},"bibbaseid":"albuquerque-anibal-desouza-deoliveira-inada-afonso-souza-pratavieira-etal-photodynamicinactivationusingcurcuminoidsandphotogemoncaenorhabditiselegans-2018","role":"author","downloads":0},"bibtype":"article","creationDate":"2019-12-17T20:34:26.232Z","downloads":0,"keywords":[],"search_terms":["photodynamic","inactivation","using","curcuminoids","photogem","caenorhabditis","elegans","albuquerque","anibal","de souza","de oliveira","inada","afonso","souza","pratavieira","bagnato"],"title":"Photodynamic inactivation using curcuminoids and Photogem on caenorhabditis elegans","year":2018}