Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories. Aliaga Fandino, A. C., Jelínková, A., Marhava, P., Petrášek, J., & Hardtke, C. S The Plant Cell, 36(5):1791–1805, May, 2024. ![Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Polar auxin transport in the Arabidopsis (Arabidopsis thaliana) root tip maintains high auxin levels around the stem cell niche that gradually decrease in dividing cells but increase again once they transition toward differentiation. Protophloem differentiates earlier than other proximal tissues and employs a unique auxin “canalization” machinery that is thought to balance auxin efflux with retention. It consists of a proposed activator of PIN-FORMED (PIN) auxin efflux carriers, the cAMP-, cGMP- and Calcium-dependent (AGC) kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX); its inhibitor, BREVIS RADIX (BRX); and PHOSPHATIDYLINOSITOL-4-PHOSPHATE-5-KINASE (PIP5K) enzymes, which promote polar PAX and BRX localization. Because of a dynamic PAX–BRX–PIP5K interplay, the net cellular output of this machinery remains unclear. In this study, we deciphered the dosage-sensitive regulatory interactions among PAX, BRX, and PIP5K by their ectopic expression in developing xylem vessels. The data suggest that the dominant collective output of the PAX–BRX–PIP5K module is a localized reduction in PIN abundance. This requires PAX-stimulated clathrin-mediated PIN endocytosis upon site-specific phosphorylation, which distinguishes PAX from other AGC kinases. An ectopic assembly of the PAX–BRX–PIP5K module is sufficient to cause cellular auxin retention and affects root growth vigor by accelerating the trajectory of xylem vessel development. Our data thus provide direct evidence that local manipulation of auxin efflux alters the timing of cellular differentiation in the root.
@article{aliaga_fandino_ectopic_2024,
title = {Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories},
volume = {36},
issn = {1040-4651},
url = {https://doi.org/10.1093/plcell/koae023},
doi = {10.1093/plcell/koae023},
abstract = {Polar auxin transport in the Arabidopsis (Arabidopsis thaliana) root tip maintains high auxin levels around the stem cell niche that gradually decrease in dividing cells but increase again once they transition toward differentiation. Protophloem differentiates earlier than other proximal tissues and employs a unique auxin “canalization” machinery that is thought to balance auxin efflux with retention. It consists of a proposed activator of PIN-FORMED (PIN) auxin efflux carriers, the cAMP-, cGMP- and Calcium-dependent (AGC) kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX); its inhibitor, BREVIS RADIX (BRX); and PHOSPHATIDYLINOSITOL-4-PHOSPHATE-5-KINASE (PIP5K) enzymes, which promote polar PAX and BRX localization. Because of a dynamic PAX–BRX–PIP5K interplay, the net cellular output of this machinery remains unclear. In this study, we deciphered the dosage-sensitive regulatory interactions among PAX, BRX, and PIP5K by their ectopic expression in developing xylem vessels. The data suggest that the dominant collective output of the PAX–BRX–PIP5K module is a localized reduction in PIN abundance. This requires PAX-stimulated clathrin-mediated PIN endocytosis upon site-specific phosphorylation, which distinguishes PAX from other AGC kinases. An ectopic assembly of the PAX–BRX–PIP5K module is sufficient to cause cellular auxin retention and affects root growth vigor by accelerating the trajectory of xylem vessel development. Our data thus provide direct evidence that local manipulation of auxin efflux alters the timing of cellular differentiation in the root.},
number = {5},
urldate = {2024-10-02},
journal = {The Plant Cell},
author = {Aliaga Fandino, Ana Cecilia and Jelínková, Adriana and Marhava, Petra and Petrášek, Jan and Hardtke, Christian S},
month = may,
year = {2024},
pages = {1791--1805},
}
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It consists of a proposed activator of PIN-FORMED (PIN) auxin efflux carriers, the cAMP-, cGMP- and Calcium-dependent (AGC) kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX); its inhibitor, BREVIS RADIX (BRX); and PHOSPHATIDYLINOSITOL-4-PHOSPHATE-5-KINASE (PIP5K) enzymes, which promote polar PAX and BRX localization. Because of a dynamic PAX–BRX–PIP5K interplay, the net cellular output of this machinery remains unclear. In this study, we deciphered the dosage-sensitive regulatory interactions among PAX, BRX, and PIP5K by their ectopic expression in developing xylem vessels. The data suggest that the dominant collective output of the PAX–BRX–PIP5K module is a localized reduction in PIN abundance. This requires PAX-stimulated clathrin-mediated PIN endocytosis upon site-specific phosphorylation, which distinguishes PAX from other AGC kinases. An ectopic assembly of the PAX–BRX–PIP5K module is sufficient to cause cellular auxin retention and affects root growth vigor by accelerating the trajectory of xylem vessel development. Our data thus provide direct evidence that local manipulation of auxin efflux alters the timing of cellular differentiation in the root.","number":"5","urldate":"2024-10-02","journal":"The Plant Cell","author":[{"propositions":[],"lastnames":["Aliaga","Fandino"],"firstnames":["Ana","Cecilia"],"suffixes":[]},{"propositions":[],"lastnames":["Jelínková"],"firstnames":["Adriana"],"suffixes":[]},{"propositions":[],"lastnames":["Marhava"],"firstnames":["Petra"],"suffixes":[]},{"propositions":[],"lastnames":["Petrášek"],"firstnames":["Jan"],"suffixes":[]},{"propositions":[],"lastnames":["Hardtke"],"firstnames":["Christian","S"],"suffixes":[]}],"month":"May","year":"2024","pages":"1791–1805","bibtex":"@article{aliaga_fandino_ectopic_2024,\n\ttitle = {Ectopic assembly of an auxin efflux control machinery shifts developmental trajectories},\n\tvolume = {36},\n\tissn = {1040-4651},\n\turl = {https://doi.org/10.1093/plcell/koae023},\n\tdoi = {10.1093/plcell/koae023},\n\tabstract = {Polar auxin transport in the Arabidopsis (Arabidopsis thaliana) root tip maintains high auxin levels around the stem cell niche that gradually decrease in dividing cells but increase again once they transition toward differentiation. Protophloem differentiates earlier than other proximal tissues and employs a unique auxin “canalization” machinery that is thought to balance auxin efflux with retention. It consists of a proposed activator of PIN-FORMED (PIN) auxin efflux carriers, the cAMP-, cGMP- and Calcium-dependent (AGC) kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX); its inhibitor, BREVIS RADIX (BRX); and PHOSPHATIDYLINOSITOL-4-PHOSPHATE-5-KINASE (PIP5K) enzymes, which promote polar PAX and BRX localization. Because of a dynamic PAX–BRX–PIP5K interplay, the net cellular output of this machinery remains unclear. In this study, we deciphered the dosage-sensitive regulatory interactions among PAX, BRX, and PIP5K by their ectopic expression in developing xylem vessels. The data suggest that the dominant collective output of the PAX–BRX–PIP5K module is a localized reduction in PIN abundance. This requires PAX-stimulated clathrin-mediated PIN endocytosis upon site-specific phosphorylation, which distinguishes PAX from other AGC kinases. An ectopic assembly of the PAX–BRX–PIP5K module is sufficient to cause cellular auxin retention and affects root growth vigor by accelerating the trajectory of xylem vessel development. Our data thus provide direct evidence that local manipulation of auxin efflux alters the timing of cellular differentiation in the root.},\n\tnumber = {5},\n\turldate = {2024-10-02},\n\tjournal = {The Plant Cell},\n\tauthor = {Aliaga Fandino, Ana Cecilia and Jelínková, Adriana and Marhava, Petra and Petrášek, Jan and Hardtke, Christian S},\n\tmonth = may,\n\tyear = {2024},\n\tpages = {1791--1805},\n}\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n","author_short":["Aliaga Fandino, A. C.","Jelínková, A.","Marhava, P.","Petrášek, J.","Hardtke, C. 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