@article{RN149,
   author = {Alinezhadbalalami, N. and Douglas, T. A. and Balani, N. and Verbridge, S. S. and Davalos, R. V.},
   title = {The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness},
   journal = {Electrophoresis},
   volume = {40},
   number = {18-19},
   pages = {2592-2600},
   note = {1522-2683
Alinezhadbalalami, Nastaran
Orcid: 0000-0003-2843-361x
Douglas, Temple A
Orcid: 0000-0002-5370-2925
Balani, Nikita
Verbridge, Scott S
Davalos, Rafael V
R01CA213423/NH/NIH HHS/United States
National Institute of Health/International
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Germany
2019/05/28
Electrophoresis. 2019 Sep;40(18-19):2592-2600. doi: 10.1002/elps.201900026. Epub 2019 Jun 6.},
   abstract = {Cancer stem cells (CSCs) are aggressive subpopulations with increased stem-like properties. CSCs are usually resistant to most standard therapies and are responsible for tumor repropagation. Similar to normal stem cells, isolation of CSCs is challenging due to the lack of reliable markers. Antigen-based sorting of CSCs usually requires staining with multiple markers, making the experiments complicated, expensive, and sometimes unreliable. Here, we study the feasibility of using dielectrophoresis (DEP) for isolation of glioblastoma cells with increased stemness. We culture a glioblastoma cell line in the form of neurospheres as an in vitro model for glioblastoma stem cells. We demonstrate that spheroid forming cells have higher expression of stem cell marker, nestin. Next, we show that dielectric properties of neurospheres change as a result of changing culture conditions. Our results indicate that spheroid forming cells need higher voltages to experience the same DEP force magnitude compared to normal monolayer cultures of glioblastoma cell line. This study confirms the possibility of using DEP to isolate glioblastoma stem cells.},
   keywords = {Cell Line, Tumor
Electrophoresis/instrumentation/*methods
Equipment Design
Feasibility Studies
Glioblastoma/*pathology
Humans
Microfluidic Analytical Techniques/instrumentation/*methods
*Spheroids, Cellular/classification/cytology
Tumor Cells, Cultured
Dielectrophoretic
Electrokinetics
Glioblastoma stem cells
Microfluidics
Spheroid culture},
   ISSN = {0173-0835},
   DOI = {10.1002/elps.201900026},
   year = {2019},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Alinezhadbalalami"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Douglas"],"firstnames":["T.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Balani"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Verbridge"],"firstnames":["S.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness","journal":"Electrophoresis","volume":"40","number":"18-19","pages":"2592-2600","note":"1522-2683 Alinezhadbalalami, Nastaran Orcid: 0000-0003-2843-361x Douglas, Temple A Orcid: 0000-0002-5370-2925 Balani, Nikita Verbridge, Scott S Davalos, Rafael V R01CA213423/NH/NIH HHS/United States National Institute of Health/International Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Germany 2019/05/28 Electrophoresis. 2019 Sep;40(18-19):2592-2600. doi: 10.1002/elps.201900026. Epub 2019 Jun 6.","abstract":"Cancer stem cells (CSCs) are aggressive subpopulations with increased stem-like properties. CSCs are usually resistant to most standard therapies and are responsible for tumor repropagation. Similar to normal stem cells, isolation of CSCs is challenging due to the lack of reliable markers. Antigen-based sorting of CSCs usually requires staining with multiple markers, making the experiments complicated, expensive, and sometimes unreliable. Here, we study the feasibility of using dielectrophoresis (DEP) for isolation of glioblastoma cells with increased stemness. We culture a glioblastoma cell line in the form of neurospheres as an in vitro model for glioblastoma stem cells. We demonstrate that spheroid forming cells have higher expression of stem cell marker, nestin. Next, we show that dielectric properties of neurospheres change as a result of changing culture conditions. Our results indicate that spheroid forming cells need higher voltages to experience the same DEP force magnitude compared to normal monolayer cultures of glioblastoma cell line. This study confirms the possibility of using DEP to isolate glioblastoma stem cells.","keywords":"Cell Line, Tumor Electrophoresis/instrumentation/*methods Equipment Design Feasibility Studies Glioblastoma/*pathology Humans Microfluidic Analytical Techniques/instrumentation/*methods *Spheroids, Cellular/classification/cytology Tumor Cells, Cultured Dielectrophoretic Electrokinetics Glioblastoma stem cells Microfluidics Spheroid culture","issn":"0173-0835","doi":"10.1002/elps.201900026","year":"2019","bibtex":"@article{RN149,\n author = {Alinezhadbalalami, N. and Douglas, T. A. and Balani, N. and Verbridge, S. S. and Davalos, R. V.},\n title = {The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness},\n journal = {Electrophoresis},\n volume = {40},\n number = {18-19},\n pages = {2592-2600},\n note = {1522-2683\nAlinezhadbalalami, Nastaran\nOrcid: 0000-0003-2843-361x\nDouglas, Temple A\nOrcid: 0000-0002-5370-2925\nBalani, Nikita\nVerbridge, Scott S\nDavalos, Rafael V\nR01CA213423/NH/NIH HHS/United States\nNational Institute of Health/International\nJournal Article\nResearch Support, N.I.H., Extramural\nResearch Support, Non-U.S. Gov't\nGermany\n2019/05/28\nElectrophoresis. 2019 Sep;40(18-19):2592-2600. doi: 10.1002/elps.201900026. Epub 2019 Jun 6.},\n abstract = {Cancer stem cells (CSCs) are aggressive subpopulations with increased stem-like properties. CSCs are usually resistant to most standard therapies and are responsible for tumor repropagation. Similar to normal stem cells, isolation of CSCs is challenging due to the lack of reliable markers. Antigen-based sorting of CSCs usually requires staining with multiple markers, making the experiments complicated, expensive, and sometimes unreliable. Here, we study the feasibility of using dielectrophoresis (DEP) for isolation of glioblastoma cells with increased stemness. We culture a glioblastoma cell line in the form of neurospheres as an in vitro model for glioblastoma stem cells. We demonstrate that spheroid forming cells have higher expression of stem cell marker, nestin. Next, we show that dielectric properties of neurospheres change as a result of changing culture conditions. Our results indicate that spheroid forming cells need higher voltages to experience the same DEP force magnitude compared to normal monolayer cultures of glioblastoma cell line. This study confirms the possibility of using DEP to isolate glioblastoma stem cells.},\n keywords = {Cell Line, Tumor\nElectrophoresis/instrumentation/*methods\nEquipment Design\nFeasibility Studies\nGlioblastoma/*pathology\nHumans\nMicrofluidic Analytical Techniques/instrumentation/*methods\n*Spheroids, Cellular/classification/cytology\nTumor Cells, Cultured\nDielectrophoretic\nElectrokinetics\nGlioblastoma stem cells\nMicrofluidics\nSpheroid culture},\n ISSN = {0173-0835},\n DOI = {10.1002/elps.201900026},\n year = {2019},\n type = {Journal Article}\n}\n\n","author_short":["Alinezhadbalalami, N.","Douglas, T. A.","Balani, N.","Verbridge, S. S.","Davalos, R. 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