{"_id":"5i6gpYeXEF7bxf3aY","bibbaseid":"alkhafaji-smith-garimella-babadi-popic-sadefeldman-gatzen-sarkizova-etal-highthroughputrnaisoformsequencingusingprogrammedcdnaconcatenation-2023","author_short":["Al'Khafaji, A. M","Smith, J. T","Garimella, K. V","Babadi, M.","Popic, V.","Sade-Feldman, M.","Gatzen, M.","Sarkizova, S.","Schwartz, M. A","Blaum, E. M","Day, A.","Costello, M.","Bowers, T.","Gabriel, S.","Banks, E.","Philippakis, A. A","Boland, G. M","Blainey, P. C","Hacohen, N."],"bibdata":{"bibtype":"article","type":"article","title":"High-throughput RNA isoform sequencing using programmed cDNA concatenation","author":[{"firstnames":["Aziz","M"],"propositions":[],"lastnames":["Al'Khafaji"],"suffixes":[]},{"firstnames":["Jonathan","T"],"propositions":[],"lastnames":["Smith"],"suffixes":[]},{"firstnames":["Kiran","V"],"propositions":[],"lastnames":["Garimella"],"suffixes":[]},{"firstnames":["Mehrtash"],"propositions":[],"lastnames":["Babadi"],"suffixes":[]},{"firstnames":["Victoria"],"propositions":[],"lastnames":["Popic"],"suffixes":[]},{"firstnames":["Moshe"],"propositions":[],"lastnames":["Sade-Feldman"],"suffixes":[]},{"firstnames":["Michael"],"propositions":[],"lastnames":["Gatzen"],"suffixes":[]},{"firstnames":["Siranush"],"propositions":[],"lastnames":["Sarkizova"],"suffixes":[]},{"firstnames":["Marc","A"],"propositions":[],"lastnames":["Schwartz"],"suffixes":[]},{"firstnames":["Emily","M"],"propositions":[],"lastnames":["Blaum"],"suffixes":[]},{"firstnames":["Allyson"],"propositions":[],"lastnames":["Day"],"suffixes":[]},{"firstnames":["Maura"],"propositions":[],"lastnames":["Costello"],"suffixes":[]},{"firstnames":["Tera"],"propositions":[],"lastnames":["Bowers"],"suffixes":[]},{"firstnames":["Stacey"],"propositions":[],"lastnames":["Gabriel"],"suffixes":[]},{"firstnames":["Eric"],"propositions":[],"lastnames":["Banks"],"suffixes":[]},{"firstnames":["Anthony","A"],"propositions":[],"lastnames":["Philippakis"],"suffixes":[]},{"firstnames":["Genevieve","M"],"propositions":[],"lastnames":["Boland"],"suffixes":[]},{"firstnames":["Paul","C"],"propositions":[],"lastnames":["Blainey"],"suffixes":[]},{"firstnames":["Nir"],"propositions":[],"lastnames":["Hacohen"],"suffixes":[]}],"doi":"10.1038/s41587-023-01815-7","issn":"1546-1696","year":"2023","date":"2023-06-01","journal":"Nat Biotechnol","abstract":"Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in the discovery of differentially spliced genes.","keywords":"","pubstate":"published","tppubtype":"article","bibtex":"@article{pmid37291427,\ntitle = {High-throughput RNA isoform sequencing using programmed cDNA concatenation},\nauthor = {Aziz M Al'Khafaji and Jonathan T Smith and Kiran V Garimella and Mehrtash Babadi and Victoria Popic and Moshe Sade-Feldman and Michael Gatzen and Siranush Sarkizova and Marc A Schwartz and Emily M Blaum and Allyson Day and Maura Costello and Tera Bowers and Stacey Gabriel and Eric Banks and Anthony A Philippakis and Genevieve M Boland and Paul C Blainey and Nir Hacohen},\ndoi = {10.1038/s41587-023-01815-7},\nissn = {1546-1696},\nyear = {2023},\ndate = {2023-06-01},\njournal = {Nat Biotechnol},\nabstract = {Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in the discovery of differentially spliced genes.},\nkeywords = {},\npubstate = {published},\ntppubtype = {article}\n}\n","author_short":["Al'Khafaji, A. M","Smith, J. T","Garimella, K. V","Babadi, M.","Popic, V.","Sade-Feldman, M.","Gatzen, M.","Sarkizova, S.","Schwartz, M. A","Blaum, E. M","Day, A.","Costello, M.","Bowers, T.","Gabriel, S.","Banks, E.","Philippakis, A. A","Boland, G. M","Blainey, P. C","Hacohen, N."],"key":"pmid37291427","id":"pmid37291427","bibbaseid":"alkhafaji-smith-garimella-babadi-popic-sadefeldman-gatzen-sarkizova-etal-highthroughputrnaisoformsequencingusingprogrammedcdnaconcatenation-2023","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/KRpSceBtgDX8h56tc","dataSources":["BR3FBurNqPdq5b7Jh","5GAofPTZnXeNstHQf","y8tT6mzHEZ8Q9E8Kd","Q5xvqKLoz8uikDzpD","hu7RM8PAjD5uaaYoh","KWQN3nHEDpQ2tZAun"],"keywords":[],"search_terms":["high","throughput","rna","isoform","sequencing","using","programmed","cdna","concatenation","al'khafaji","smith","garimella","babadi","popic","sade-feldman","gatzen","sarkizova","schwartz","blaum","day","costello","bowers","gabriel","banks","philippakis","boland","blainey","hacohen"],"title":"High-throughput RNA isoform sequencing using programmed cDNA concatenation","year":2023}