FRET templated by G-quadruplex DNA: a specific ternary interaction using an original pair of donor/acceptor partners. Allain, C., Monchaud, D., & Teulade-Fichou, M. Journal of the American Chemical Society, 128(36):11890–3, September, 2006. Paper doi abstract bibtex G-quadruplex represents a suitable scaffold for FRET (fluorescence resonance energy transfer) since its two external quartets offer two well-defined binding sites for concomitant trapping of donor/acceptor partners. Combining selective G-quadruplex binders (macrocyclic bis(quinacridine) BOQ(1) or monomeric quinacridine MMQ(1), donor) with a highly fluorescent DNA probe (thiazole orange, acceptor), we designed a structure-specific FRET-system based on an unprecedented noncovalent ternary complex. This system could be potentially usable as a signature for quadruplex-DNA conformation in solution, but also might offer a unique means for observing cation and ligand binding influence on quadruplex topology.
@article{Allain2006,
title = {{FRET} templated by {G}-quadruplex {DNA}: a specific ternary interaction using an original pair of donor/acceptor partners.},
volume = {128},
issn = {0002-7863},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16953629},
doi = {10.1021/ja062193h},
abstract = {G-quadruplex represents a suitable scaffold for FRET (fluorescence resonance energy transfer) since its two external quartets offer two well-defined binding sites for concomitant trapping of donor/acceptor partners. Combining selective G-quadruplex binders (macrocyclic bis(quinacridine) BOQ(1) or monomeric quinacridine MMQ(1), donor) with a highly fluorescent DNA probe (thiazole orange, acceptor), we designed a structure-specific FRET-system based on an unprecedented noncovalent ternary complex. This system could be potentially usable as a signature for quadruplex-DNA conformation in solution, but also might offer a unique means for observing cation and ligand binding influence on quadruplex topology.},
number = {36},
journal = {Journal of the American Chemical Society},
author = {Allain, Clémence and Monchaud, David and Teulade-Fichou, Marie-Paule},
month = sep,
year = {2006},
pmid = {16953629},
keywords = {\#nosource, Acridines, Acridines: chemistry, Benzothiazoles, Benzothiazoles: chemistry, Binding Sites, DNA, DNA Probes, DNA Probes: chemistry, DNA: chemistry, Fluorescence, Fluorescence Resonance Energy Transfer, Fluorescence Resonance Energy Transfer: methods, Fluorescent Dyes, Fluorescent Dyes: chemistry, G-Quadruplexes, Nucleic Acid Conformation, Quinolines, Quinolines: chemistry, Spectrometry},
pages = {11890--3},
}
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