Thermodynamics of internal C.T mismatches in DNA. Allawi, H T & SantaLucia, J Nucleic acids research, 26(11):2694–701, June, 1998. ISBN: 1058110594![Thermodynamics of internal C.T mismatches in DNA. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4%, 9.9%, 10.6%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.
@article{Allawi1998,
title = {Thermodynamics of internal {C}.{T} mismatches in {DNA}.},
volume = {26},
issn = {0305-1048},
url = {http://www.ncbi.nlm.nih.gov/pubmed/10090733},
abstract = {Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4\%, 9.9\%, 10.6\%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.},
number = {11},
journal = {Nucleic acids research},
author = {Allawi, H T and SantaLucia, J},
month = jun,
year = {1998},
pmid = {9592156},
note = {ISBN: 1058110594},
keywords = {\#nosource, Biomolecular, Cytidine, DNA, DNA: chemistry, Hydrogen-Ion Concentration, Nuclear Magnetic Resonance, Nucleic Acid Heteroduplexes, Thermodynamics, Thymidine},
pages = {2694--701},
}
Downloads: 0
{"_id":"3NEtgX84k3QWB8kZw","bibbaseid":"allawi-santalucia-thermodynamicsofinternalctmismatchesindna-1998","author_short":["Allawi, H T","SantaLucia, J"],"bibdata":{"bibtype":"article","type":"article","title":"Thermodynamics of internal C.T mismatches in DNA.","volume":"26","issn":"0305-1048","url":"http://www.ncbi.nlm.nih.gov/pubmed/10090733","abstract":"Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4%, 9.9%, 10.6%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.","number":"11","journal":"Nucleic acids research","author":[{"propositions":[],"lastnames":["Allawi"],"firstnames":["H","T"],"suffixes":[]},{"propositions":[],"lastnames":["SantaLucia"],"firstnames":["J"],"suffixes":[]}],"month":"June","year":"1998","pmid":"9592156","note":"ISBN: 1058110594","keywords":"#nosource, Biomolecular, Cytidine, DNA, DNA: chemistry, Hydrogen-Ion Concentration, Nuclear Magnetic Resonance, Nucleic Acid Heteroduplexes, Thermodynamics, Thymidine","pages":"2694–701","bibtex":"@article{Allawi1998,\n\ttitle = {Thermodynamics of internal {C}.{T} mismatches in {DNA}.},\n\tvolume = {26},\n\tissn = {0305-1048},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/10090733},\n\tabstract = {Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4\\%, 9.9\\%, 10.6\\%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.},\n\tnumber = {11},\n\tjournal = {Nucleic acids research},\n\tauthor = {Allawi, H T and SantaLucia, J},\n\tmonth = jun,\n\tyear = {1998},\n\tpmid = {9592156},\n\tnote = {ISBN: 1058110594},\n\tkeywords = {\\#nosource, Biomolecular, Cytidine, DNA, DNA: chemistry, Hydrogen-Ion Concentration, Nuclear Magnetic Resonance, Nucleic Acid Heteroduplexes, Thermodynamics, Thymidine},\n\tpages = {2694--701},\n}\n\n","author_short":["Allawi, H T","SantaLucia, J"],"key":"Allawi1998","id":"Allawi1998","bibbaseid":"allawi-santalucia-thermodynamicsofinternalctmismatchesindna-1998","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/10090733"},"keyword":["#nosource","Biomolecular","Cytidine","DNA","DNA: chemistry","Hydrogen-Ion Concentration","Nuclear Magnetic Resonance","Nucleic Acid Heteroduplexes","Thermodynamics","Thymidine"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":["#nosource","biomolecular","cytidine","dna","dna: chemistry","hydrogen-ion concentration","nuclear magnetic resonance","nucleic acid heteroduplexes","thermodynamics","thymidine"],"search_terms":["thermodynamics","internal","mismatches","dna","allawi","santalucia"],"title":"Thermodynamics of internal C.T mismatches in DNA.","year":1998}