{"_id":"M5neTLjpnbo5yBCRC","bibbaseid":"alles-ludwig-rheinheimer-leidinger-grsser-keller-meese-mir148aimpairsraserksignalinginblymphocytesbytargetingsosproteins-2017","downloads":0,"creationDate":"2018-07-24T15:09:56.848Z","title":"MiR-148a impairs Ras/ERK signaling in B lymphocytes by targeting SOS proteins.","author_short":["Alles, J.","Ludwig, N.","Rheinheimer, S.","Leidinger, P.","Grässer, F. A","Keller, A.","Meese, E."],"year":2017,"bibtype":"article","biburl":"https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Alles"],"firstnames":["Julia"],"suffixes":[]},{"propositions":[],"lastnames":["Ludwig"],"firstnames":["Nicole"],"suffixes":[]},{"propositions":[],"lastnames":["Rheinheimer"],"firstnames":["Stefanie"],"suffixes":[]},{"propositions":[],"lastnames":["Leidinger"],"firstnames":["Petra"],"suffixes":[]},{"propositions":[],"lastnames":["Grässer"],"firstnames":["Friedrich","A"],"suffixes":[]},{"propositions":[],"lastnames":["Keller"],"firstnames":["Andreas"],"suffixes":[]},{"propositions":[],"lastnames":["Meese"],"firstnames":["Eckart"],"suffixes":[]}],"title":"MiR-148a impairs Ras/ERK signaling in B lymphocytes by targeting SOS proteins.","journal":"Oncotarget","year":"2017","volume":"8","pages":"56417–56427","month":"August","issn":"1949-2553","abstract":"Although microRNAs have been recognized as central cellular regulators, there is an evident lack of knowledge about their targets. Here, we analyzed potential target genes for miR-148a functioning in Ras signaling in B cells, including SOS1 and SOS2. A dual-luciferase reporter assay showed significantly decreased luciferase activity upon ectopic overexpression of miR-148a in HEK-293T cells that were co-transfected with the 3'UTR of either SOS1 or SOS2. Each of the 3'UTRs of SOS1 and SOS2 contained two binding sites for miR-148a both of which were necessary for the decreased luciferase activity. MiR-148a overexpression in HEK-293T lead to significantly reduced levels of both endogenous SOS1 and SOS2 proteins. Likewise, reduced levels of SOS proteins were found in two B cell lines that were transfected with miR-148a. The level of ERK1/2 phosphorylation as one of the most relevant downstream members of the Ras/ERK signaling pathway was also reduced in cells with miR-148a overexpression. The data show that miR-148a impairs the Ras/ERK signaling pathway via SOS1 and SOS2 proteins in B cells.","completed":"2017-09-25","country":"United States","doi":"10.18632/oncotarget.17662","issn-linking":"1949-2553","issue":"34","keywords":"B cells; ERK; SOS; miR-148a; microRNA","nlm-id":"101532965","owner":"NLM","pii":"17662","pmc":"PMC5593572","pmid":"28915601","pubmodel":"Electronic-eCollection","pubstatus":"epublish","revised":"2017-09-25","bibtex":"@Article{Alles2017,\n author = {Alles, Julia and Ludwig, Nicole and Rheinheimer, Stefanie and Leidinger, Petra and Grässer, Friedrich A and Keller, Andreas and Meese, Eckart},\n title = {MiR-148a impairs Ras/ERK signaling in B lymphocytes by targeting SOS proteins.},\n journal = {Oncotarget},\n year = {2017},\n volume = {8},\n pages = {56417--56427},\n month = aug,\n issn = {1949-2553},\n abstract = {Although microRNAs have been recognized as central cellular regulators, there is an evident lack of knowledge about their targets. Here, we analyzed potential target genes for miR-148a functioning in Ras signaling in B cells, including SOS1 and SOS2. A dual-luciferase reporter assay showed significantly decreased luciferase activity upon ectopic overexpression of miR-148a in HEK-293T cells that were co-transfected with the 3'UTR of either SOS1 or SOS2. Each of the 3'UTRs of SOS1 and SOS2 contained two binding sites for miR-148a both of which were necessary for the decreased luciferase activity. MiR-148a overexpression in HEK-293T lead to significantly reduced levels of both endogenous SOS1 and SOS2 proteins. Likewise, reduced levels of SOS proteins were found in two B cell lines that were transfected with miR-148a. The level of ERK1/2 phosphorylation as one of the most relevant downstream members of the Ras/ERK signaling pathway was also reduced in cells with miR-148a overexpression. The data show that miR-148a impairs the Ras/ERK signaling pathway via SOS1 and SOS2 proteins in B cells.},\n completed = {2017-09-25},\n country = {United States},\n doi = {10.18632/oncotarget.17662},\n issn-linking = {1949-2553},\n issue = {34},\n keywords = {B cells; ERK; SOS; miR-148a; microRNA},\n nlm-id = {101532965},\n owner = {NLM},\n pii = {17662},\n pmc = {PMC5593572},\n pmid = {28915601},\n pubmodel = {Electronic-eCollection},\n pubstatus = {epublish},\n revised = {2017-09-25},\n}\n\n","author_short":["Alles, J.","Ludwig, N.","Rheinheimer, S.","Leidinger, P.","Grässer, F. A","Keller, A.","Meese, E."],"key":"Alles2017","id":"Alles2017","bibbaseid":"alles-ludwig-rheinheimer-leidinger-grsser-keller-meese-mir148aimpairsraserksignalinginblymphocytesbytargetingsosproteins-2017","role":"author","urls":{},"keyword":["B cells; ERK; SOS; miR-148a; microRNA"],"metadata":{"authorlinks":{"keller, a":"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2023/07/references.bib_.txt&folding=1"}},"html":""},"search_terms":["mir","148a","impairs","ras","erk","signaling","lymphocytes","targeting","sos","proteins","alles","ludwig","rheinheimer","leidinger","grässer","keller","meese"],"keywords":["b cells; erk; sos; mir-148a; microrna"],"authorIDs":["uqPgPxPv8wujtk7HZ"],"dataSources":["Tk7NyW85uR28Rhd26","k7tjjxqz46TBRgack","qqBiPXk2jEroaRXH2","9DxWazzLQoAjp9mw3","MaeSQYhi8jBE6oYaK","XSoPwnytNRZeNL8Wv","ukDDkYqwLbdhYXTJA","qd2NgSKHS68Kcdt7y","uFrEYNpx3Zmayo2AS","X7BjFZrHHnyywjGc5","BD2qbudjMvyXtTiz5","NmhXQcJvRc2QhnSZF","ipvH6pWABxuwdKDLx","Pny5E4E9kc7C8gG8g","SiGP46KPWizw6ihLJ"]}