@article{RN240,
   author = {Al-Sakere, B. and André, F. and Bernat, C. and Connault, E. and Opolon, P. and Davalos, R. V. and Rubinsky, B. and Mir, L. M.},
   title = {Tumor ablation with irreversible electroporation},
   journal = {PLoS One},
   volume = {2},
   number = {11},
   pages = {e1135},
   note = {1932-6203
Al-Sakere, Bassim
André, Franck
Bernat, Claire
Connault, Elisabeth
Opolon, Paule
Davalos, Rafael V
Rubinsky, Boris
Mir, Lluis M
R01 RR018961/RR/NCRR NIH HHS/United States
R01 RR018961-04/RR/NCRR NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
United States
2007/11/09
PLoS One. 2007 Nov 7;2(11):e1135. doi: 10.1371/journal.pone.0001135.},
   abstract = {We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 micros at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.},
   keywords = {Animals
Cell Line, Tumor
DNA Damage
*Electroporation
Immunohistochemistry
In Situ Nick-End Labeling
Mice
Mice, Inbred C57BL
Sarcoma, Experimental/*therapy
Temperature},
   ISSN = {1932-6203},
   DOI = {10.1371/journal.pone.0001135},
   year = {2007},
   type = {Journal Article}
}

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M."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Al-Sakere"],"firstnames":["B."],"suffixes":[]},{"propositions":[],"lastnames":["André"],"firstnames":["F."],"suffixes":[]},{"propositions":[],"lastnames":["Bernat"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Connault"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Opolon"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Rubinsky"],"firstnames":["B."],"suffixes":[]},{"propositions":[],"lastnames":["Mir"],"firstnames":["L.","M."],"suffixes":[]}],"title":"Tumor ablation with irreversible electroporation","journal":"PLoS One","volume":"2","number":"11","pages":"e1135","note":"1932-6203 Al-Sakere, Bassim André, Franck Bernat, Claire Connault, Elisabeth Opolon, Paule Davalos, Rafael V Rubinsky, Boris Mir, Lluis M R01 RR018961/RR/NCRR NIH HHS/United States R01 RR018961-04/RR/NCRR NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't United States 2007/11/09 PLoS One. 2007 Nov 7;2(11):e1135. doi: 10.1371/journal.pone.0001135.","abstract":"We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 micros at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.","keywords":"Animals Cell Line, Tumor DNA Damage *Electroporation Immunohistochemistry In Situ Nick-End Labeling Mice Mice, Inbred C57BL Sarcoma, Experimental/*therapy Temperature","issn":"1932-6203","doi":"10.1371/journal.pone.0001135","year":"2007","bibtex":"@article{RN240,\n author = {Al-Sakere, B. and André, F. and Bernat, C. and Connault, E. and Opolon, P. and Davalos, R. V. and Rubinsky, B. and Mir, L. M.},\n title = {Tumor ablation with irreversible electroporation},\n journal = {PLoS One},\n volume = {2},\n number = {11},\n pages = {e1135},\n note = {1932-6203\nAl-Sakere, Bassim\nAndré, Franck\nBernat, Claire\nConnault, Elisabeth\nOpolon, Paule\nDavalos, Rafael V\nRubinsky, Boris\nMir, Lluis M\nR01 RR018961/RR/NCRR NIH HHS/United States\nR01 RR018961-04/RR/NCRR NIH HHS/United States\nJournal Article\nResearch Support, N.I.H., Extramural\nResearch Support, Non-U.S. Gov't\nUnited States\n2007/11/09\nPLoS One. 2007 Nov 7;2(11):e1135. doi: 10.1371/journal.pone.0001135.},\n abstract = {We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 micros at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.},\n keywords = {Animals\nCell Line, Tumor\nDNA Damage\n*Electroporation\nImmunohistochemistry\nIn Situ Nick-End Labeling\nMice\nMice, Inbred C57BL\nSarcoma, Experimental/*therapy\nTemperature},\n ISSN = {1932-6203},\n DOI = {10.1371/journal.pone.0001135},\n year = {2007},\n type = {Journal Article}\n}\n\n","author_short":["Al-Sakere, B.","André, F.","Bernat, C.","Connault, E.","Opolon, P.","Davalos, R. V.","Rubinsky, B.","Mir, L. M."],"key":"RN240","id":"RN240","bibbaseid":"alsakere-andr-bernat-connault-opolon-davalos-rubinsky-mir-tumorablationwithirreversibleelectroporation-2007","role":"author","urls":{},"keyword":["Animals Cell Line","Tumor DNA Damage *Electroporation Immunohistochemistry In Situ Nick-End Labeling Mice Mice","Inbred C57BL Sarcoma","Experimental/*therapy Temperature"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["zaL9693Ha8EYPcn6R","FWKuFu88GYvaFfxyp","wnTqRCfBSz2AkAxJo","BWcnpMbDFTWngT8TX","ntzd2BXQzDvcuBAni","ud8ZKuHT43FjZJtFt","oFDkexDhXJBsNbdmC","dwEFKjxyXZxuiMwXp","D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals cell line","tumor dna damage *electroporation immunohistochemistry in situ nick-end labeling mice mice","inbred c57bl sarcoma","experimental/*therapy temperature"],"search_terms":["tumor","ablation","irreversible","electroporation","al-sakere","andré","bernat","connault","opolon","davalos","rubinsky","mir"],"title":"Tumor ablation with irreversible electroporation","year":2007}