Tumor Ablation with Irreversible Electroporation. Al-Sakere, B., Andre, F., Bernat, C., Connault, E., Opolon, P., Davalos, R. V., Rubinsky, B., & Mir, L. M. PLOS ONE, PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA, NOV 7, 2007. doi abstract bibtex We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 mu s at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.
@article{ WOS:000207459000019,
Author = {Al-Sakere, Bassim and Andre, Franck and Bernat, Claire and Connault,
Elisabeth and Opolon, Paule and Davalos, Rafael V. and Rubinsky, Boris
and Mir, Lluis M.},
Title = {Tumor Ablation with Irreversible Electroporation},
Journal = {PLOS ONE},
Year = {2007},
Volume = {2},
Number = {11},
Month = {NOV 7},
Abstract = {We report the first successful use of irreversible electroporation for
the minimally invasive treatment of aggressive cutaneous tumors
implanted in mice. Irreversible electroporation is a newly developed
non-thermal tissue ablation technique in which certain short duration
electrical fields are used to permanently permeabilize the cell
membrane, presumably through the formation of nanoscale defects in the
cell membrane. Mathematical models of the electrical and thermal fields
that develop during the application of the pulses were used to design an
efficient treatment protocol with minimal heating of the tissue. Tumor
regression was confirmed by histological studies which also revealed
that it occurred as a direct result of irreversible cell membrane
permeabilization. Parametric studies show that the successful outcome of
the procedure is related to the applied electric field strength, the
total pulse duration as well as the temporal mode of delivery of the
pulses. Our best results were obtained using plate electrodes to deliver
across the tumor 80 pulses of 100 mu s at 0.3 Hz with an electrical
field magnitude of 2500 V/cm. These conditions induced complete
regression in 12 out of 13 treated tumors, (92\%), in the absence of
tissue heating. Irreversible electroporation is thus a new effective
modality for non-thermal tumor ablation.},
Publisher = {PUBLIC LIBRARY SCIENCE},
Address = {1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA},
Type = {Article},
Language = {English},
Affiliation = {Mir, LM (Corresponding Author), Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France.
Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France.
Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Univ Paris Sud, UMR 8121, Villejuif, France.
Davalos, Rafael V., Virginia Tech Wake Forest Univ, Sch Biomed Engn \& Sci, Blacksburg, VA USA.
Rubinsky, Boris, Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA.
Rubinsky, Boris, Univ Calif Berkeley, Grad Program Biophys, Berkeley, CA 94720 USA.
Rubinsky, Boris, Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA.
Rubinsky, Boris, Hebrew Univ Jerusalem, Sch Comp Sci \& Engn, Ctr Bioengn Serv Humanity \& Soc, Jerusalem, Israel.},
DOI = {10.1371/journal.pone.0001135},
Article-Number = {e1135},
ISSN = {1932-6203},
Research-Areas = {Science \& Technology - Other Topics},
Web-of-Science-Categories = {Multidisciplinary Sciences},
Author-Email = {luismir@igr.fr},
Affiliations = {Centre National de la Recherche Scientifique (CNRS); UNICANCER; Gustave
Roussy; Universite Paris Saclay; Virginia Polytechnic Institute \& State
University; University of California System; University of California
Berkeley; University of California System; University of California
Berkeley; University of California System; University of California
Berkeley; Hebrew University of Jerusalem},
ResearcherID-Numbers = {Rubinsky, Boris/B-4439-2010
MIR, Lluis M/AAJ-9110-2020
Davalos, Rafael V/F-9012-2011
Sano, Michael B/E-1715-2011
},
ORCID-Numbers = {, Lluis/0000-0002-8671-9467},
Funding-Acknowledgement = {CNRS; IGR; U. S. National Institutes of Health (NIH) {[}NIH R01
RR018961]},
Funding-Text = {This work was supported by grants of CNRS and IGR. BR was supported in
part by the U. S. National Institutes of Health (NIH) under Grant NIH
R01 RR018961. The sponsors had no role in study design, data collection
and analysis, decision to publish, or preparation of the manuscript.},
Number-of-Cited-References = {44},
Times-Cited = {358},
Usage-Count-Last-180-days = {1},
Usage-Count-Since-2013 = {33},
Journal-ISO = {PLoS One},
Doc-Delivery-Number = {V10IZ},
Web-of-Science-Index = {Science Citation Index Expanded (SCI-EXPANDED)},
Unique-ID = {WOS:000207459000019},
OA = {gold, Green Published},
DA = {2024-03-03},
}
Downloads: 0
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M."],"bibdata":{"bibtype":"article","type":"Article","author":[{"propositions":[],"lastnames":["Al-Sakere"],"firstnames":["Bassim"],"suffixes":[]},{"propositions":[],"lastnames":["Andre"],"firstnames":["Franck"],"suffixes":[]},{"propositions":[],"lastnames":["Bernat"],"firstnames":["Claire"],"suffixes":[]},{"propositions":[],"lastnames":["Connault"],"firstnames":["Elisabeth"],"suffixes":[]},{"propositions":[],"lastnames":["Opolon"],"firstnames":["Paule"],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["Rafael","V."],"suffixes":[]},{"propositions":[],"lastnames":["Rubinsky"],"firstnames":["Boris"],"suffixes":[]},{"propositions":[],"lastnames":["Mir"],"firstnames":["Lluis","M."],"suffixes":[]}],"title":"Tumor Ablation with Irreversible Electroporation","journal":"PLOS ONE","year":"2007","volume":"2","number":"11","month":"NOV 7","abstract":"We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice. Irreversible electroporation is a newly developed non-thermal tissue ablation technique in which certain short duration electrical fields are used to permanently permeabilize the cell membrane, presumably through the formation of nanoscale defects in the cell membrane. Mathematical models of the electrical and thermal fields that develop during the application of the pulses were used to design an efficient treatment protocol with minimal heating of the tissue. Tumor regression was confirmed by histological studies which also revealed that it occurred as a direct result of irreversible cell membrane permeabilization. Parametric studies show that the successful outcome of the procedure is related to the applied electric field strength, the total pulse duration as well as the temporal mode of delivery of the pulses. Our best results were obtained using plate electrodes to deliver across the tumor 80 pulses of 100 mu s at 0.3 Hz with an electrical field magnitude of 2500 V/cm. These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation.","publisher":"PUBLIC LIBRARY SCIENCE","address":"1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA","language":"English","affiliation":"Mir, LM (Corresponding Author), Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France. Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France. Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Univ Paris Sud, UMR 8121, Villejuif, France. Davalos, Rafael V., Virginia Tech Wake Forest Univ, Sch Biomed Engn & Sci, Blacksburg, VA USA. Rubinsky, Boris, Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA. Rubinsky, Boris, Univ Calif Berkeley, Grad Program Biophys, Berkeley, CA 94720 USA. Rubinsky, Boris, Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA. 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Irreversible electroporation is a newly developed\n non-thermal tissue ablation technique in which certain short duration\n electrical fields are used to permanently permeabilize the cell\n membrane, presumably through the formation of nanoscale defects in the\n cell membrane. Mathematical models of the electrical and thermal fields\n that develop during the application of the pulses were used to design an\n efficient treatment protocol with minimal heating of the tissue. Tumor\n regression was confirmed by histological studies which also revealed\n that it occurred as a direct result of irreversible cell membrane\n permeabilization. Parametric studies show that the successful outcome of\n the procedure is related to the applied electric field strength, the\n total pulse duration as well as the temporal mode of delivery of the\n pulses. Our best results were obtained using plate electrodes to deliver\n across the tumor 80 pulses of 100 mu s at 0.3 Hz with an electrical\n field magnitude of 2500 V/cm. These conditions induced complete\n regression in 12 out of 13 treated tumors, (92\\%), in the absence of\n tissue heating. Irreversible electroporation is thus a new effective\n modality for non-thermal tumor ablation.},\nPublisher = {PUBLIC LIBRARY SCIENCE},\nAddress = {1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA},\nType = {Article},\nLanguage = {English},\nAffiliation = {Mir, LM (Corresponding Author), Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France.\n Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Inst Gustave Roussy, CNRS, UMR 8121, Villejuif, France.\n Al-Sakere, Bassim; Andre, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Mir, Lluis M., Univ Paris Sud, UMR 8121, Villejuif, France.\n Davalos, Rafael V., Virginia Tech Wake Forest Univ, Sch Biomed Engn \\& Sci, Blacksburg, VA USA.\n Rubinsky, Boris, Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA.\n Rubinsky, Boris, Univ Calif Berkeley, Grad Program Biophys, Berkeley, CA 94720 USA.\n Rubinsky, Boris, Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA.\n Rubinsky, Boris, Hebrew Univ Jerusalem, Sch Comp Sci \\& Engn, Ctr Bioengn Serv Humanity \\& Soc, Jerusalem, Israel.},\nDOI = {10.1371/journal.pone.0001135},\nArticle-Number = {e1135},\nISSN = {1932-6203},\nResearch-Areas = {Science \\& Technology - Other Topics},\nWeb-of-Science-Categories = {Multidisciplinary Sciences},\nAuthor-Email = {luismir@igr.fr},\nAffiliations = {Centre National de la Recherche Scientifique (CNRS); UNICANCER; Gustave\n Roussy; Universite Paris Saclay; Virginia Polytechnic Institute \\& State\n University; University of California System; University of California\n Berkeley; University of California System; University of California\n Berkeley; University of California System; University of California\n Berkeley; Hebrew University of Jerusalem},\nResearcherID-Numbers = {Rubinsky, Boris/B-4439-2010\n MIR, Lluis M/AAJ-9110-2020\n Davalos, Rafael V/F-9012-2011\n Sano, Michael B/E-1715-2011\n },\nORCID-Numbers = {, Lluis/0000-0002-8671-9467},\nFunding-Acknowledgement = {CNRS; IGR; U. 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