Topology of a DNA G-quadruplex structure formed in the HIV-1 promoter: a potential target for anti-HIV drug development.

Topology of a DNA G-quadruplex structure formed in the HIV-1 promoter: a potential target for anti-HIV drug development. Amrane, S., Kerkour, A., Bedrat, A., Vialet, B., Andreola, M., & Mergny, J. Journal of the American Chemical Society, 136(14):5249–52, April, 2014. tex.ids= amraneTopologyDNAGQuadruplex2014, amraneTopologyDNAGQuadruplex2014a

Paper doi abstract bibtex

Nucleic acid sequences containing guanine tracts are able to adopt noncanonical four-stranded nucleic acid structures called G-quadruplexes (G4s). These structures are based on the stacking of two or more G-tetrads; each tetrad is a planar association of four guanines held together by eight hydrogen bonds. In this study, we analyzed a conserved G-rich region from HIV-1 promoter that is known to regulate the transcription of the HIV-1 provirus. Strikingly, our analysis of an alignment of 1684 HIV-1 sequences from this region showed a high conservation of the ability to form G4 structures despite a lower conservation of the nucleotide primary sequence. Using NMR spectroscopy, we determined the G4 topology adopted by a DNA sequence from this region (HIV-PRO1: 5' TGGCCTGGGCGGGACTGGG 3'). This DNA fragment formed a stable two G-tetrad antiparallel G4 with an additional Watson-Crick CG base pair. This hybrid structure may be critical for HIV-1 gene expression and is potentially a novel target for anti-HIV-1 drug development.

@article{Amrane2014,
	title = {Topology of a {DNA} {G}-quadruplex structure formed in the {HIV}-1 promoter: a potential target for anti-{HIV} drug development.},
	volume = {136},
	issn = {1520-5126},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24649937},
	doi = {10.1021/ja501500c},
	abstract = {Nucleic acid sequences containing guanine tracts are able to adopt noncanonical four-stranded nucleic acid structures called G-quadruplexes (G4s). These structures are based on the stacking of two or more G-tetrads; each tetrad is a planar association of four guanines held together by eight hydrogen bonds. In this study, we analyzed a conserved G-rich region from HIV-1 promoter that is known to regulate the transcription of the HIV-1 provirus. Strikingly, our analysis of an alignment of 1684 HIV-1 sequences from this region showed a high conservation of the ability to form G4 structures despite a lower conservation of the nucleotide primary sequence. Using NMR spectroscopy, we determined the G4 topology adopted by a DNA sequence from this region (HIV-PRO1: 5' TGGCCTGGGCGGGACTGGG 3'). This DNA fragment formed a stable two G-tetrad antiparallel G4 with an additional Watson-Crick CG base pair. This hybrid structure may be critical for HIV-1 gene expression and is potentially a novel target for anti-HIV-1 drug development.},
	number = {14},
	journal = {Journal of the American Chemical Society},
	author = {Amrane, Samir and Kerkour, Abdelaziz and Bedrat, Amina and Vialet, Brune and Andreola, Marie-Line and Mergny, Jean-Louis},
	month = apr,
	year = {2014},
	pmid = {24649937},
	note = {tex.ids= amraneTopologyDNAGQuadruplex2014, amraneTopologyDNAGQuadruplex2014a},
	pages = {5249--52},
}

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