{"_id":"7jJTfBgPDdCFvFKq2","bibbaseid":"amrane-kerkour-bedrat-vialet-andreola-mergny-topologyofadnagquadruplexstructureformedinthehiv1promoterapotentialtargetforantihivdrugdevelopment-2014","author_short":["Amrane, S.","Kerkour, A.","Bedrat, A.","Vialet, B.","Andreola, M.","Mergny, J."],"bibdata":{"bibtype":"article","type":"article","title":"Topology of a DNA G-quadruplex structure formed in the HIV-1 promoter: a potential target for anti-HIV drug development.","volume":"136","issn":"1520-5126","url":"http://www.ncbi.nlm.nih.gov/pubmed/24649937","doi":"10.1021/ja501500c","abstract":"Nucleic acid sequences containing guanine tracts are able to adopt noncanonical four-stranded nucleic acid structures called G-quadruplexes (G4s). These structures are based on the stacking of two or more G-tetrads; each tetrad is a planar association of four guanines held together by eight hydrogen bonds. In this study, we analyzed a conserved G-rich region from HIV-1 promoter that is known to regulate the transcription of the HIV-1 provirus. Strikingly, our analysis of an alignment of 1684 HIV-1 sequences from this region showed a high conservation of the ability to form G4 structures despite a lower conservation of the nucleotide primary sequence. Using NMR spectroscopy, we determined the G4 topology adopted by a DNA sequence from this region (HIV-PRO1: 5' TGGCCTGGGCGGGACTGGG 3'). This DNA fragment formed a stable two G-tetrad antiparallel G4 with an additional Watson-Crick CG base pair. This hybrid structure may be critical for HIV-1 gene expression and is potentially a novel target for anti-HIV-1 drug development.","number":"14","journal":"Journal of the American Chemical Society","author":[{"propositions":[],"lastnames":["Amrane"],"firstnames":["Samir"],"suffixes":[]},{"propositions":[],"lastnames":["Kerkour"],"firstnames":["Abdelaziz"],"suffixes":[]},{"propositions":[],"lastnames":["Bedrat"],"firstnames":["Amina"],"suffixes":[]},{"propositions":[],"lastnames":["Vialet"],"firstnames":["Brune"],"suffixes":[]},{"propositions":[],"lastnames":["Andreola"],"firstnames":["Marie-Line"],"suffixes":[]},{"propositions":[],"lastnames":["Mergny"],"firstnames":["Jean-Louis"],"suffixes":[]}],"month":"April","year":"2014","pmid":"24649937","note":"tex.ids= amraneTopologyDNAGQuadruplex2014, amraneTopologyDNAGQuadruplex2014a","pages":"5249–52","bibtex":"@article{Amrane2014,\n\ttitle = {Topology of a {DNA} {G}-quadruplex structure formed in the {HIV}-1 promoter: a potential target for anti-{HIV} drug development.},\n\tvolume = {136},\n\tissn = {1520-5126},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/24649937},\n\tdoi = {10.1021/ja501500c},\n\tabstract = {Nucleic acid sequences containing guanine tracts are able to adopt noncanonical four-stranded nucleic acid structures called G-quadruplexes (G4s). These structures are based on the stacking of two or more G-tetrads; each tetrad is a planar association of four guanines held together by eight hydrogen bonds. In this study, we analyzed a conserved G-rich region from HIV-1 promoter that is known to regulate the transcription of the HIV-1 provirus. Strikingly, our analysis of an alignment of 1684 HIV-1 sequences from this region showed a high conservation of the ability to form G4 structures despite a lower conservation of the nucleotide primary sequence. Using NMR spectroscopy, we determined the G4 topology adopted by a DNA sequence from this region (HIV-PRO1: 5' TGGCCTGGGCGGGACTGGG 3'). This DNA fragment formed a stable two G-tetrad antiparallel G4 with an additional Watson-Crick CG base pair. This hybrid structure may be critical for HIV-1 gene expression and is potentially a novel target for anti-HIV-1 drug development.},\n\tnumber = {14},\n\tjournal = {Journal of the American Chemical Society},\n\tauthor = {Amrane, Samir and Kerkour, Abdelaziz and Bedrat, Amina and Vialet, Brune and Andreola, Marie-Line and Mergny, Jean-Louis},\n\tmonth = apr,\n\tyear = {2014},\n\tpmid = {24649937},\n\tnote = {tex.ids= amraneTopologyDNAGQuadruplex2014, amraneTopologyDNAGQuadruplex2014a},\n\tpages = {5249--52},\n}\n\n","author_short":["Amrane, S.","Kerkour, A.","Bedrat, A.","Vialet, B.","Andreola, M.","Mergny, J."],"key":"Amrane2014","id":"Amrane2014","bibbaseid":"amrane-kerkour-bedrat-vialet-andreola-mergny-topologyofadnagquadruplexstructureformedinthehiv1promoterapotentialtargetforantihivdrugdevelopment-2014","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/24649937"},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":[],"search_terms":["topology","dna","quadruplex","structure","formed","hiv","promoter","potential","target","anti","hiv","drug","development","amrane","kerkour","bedrat","vialet","andreola","mergny"],"title":"Topology of a DNA G-quadruplex structure formed in the HIV-1 promoter: a potential target for anti-HIV drug development.","year":2014}