Report on the First Detection of Pectenotoxin-2, Spirolide-A and Their Derivatives in French Shellfish. Amzil, Z., Sibat, M., Royer, F., Masson, N., & Abadie, E. 5(4):168–179. Number: 4
Report on the First Detection of Pectenotoxin-2, Spirolide-A and Their Derivatives in French Shellfish [link]Paper  doi  abstract   bibtex   
In the context of the French Phytoplankton and Phycotoxins MonitoringNetwork (REPHY) programme, shellfish samples were harvested from different locationswhere harmful algae blooms were known to have occurred. For all shellfish samples foundpositive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquidchromatography (LC) coupled with mass spectrometry (MS) was used to search for thefollowing lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins(PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines(GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3),alkaline hydrolysis was performed on all samples, and LC/MS analyses were carried out onthe samples before and after hydrolysis. The results revealed different lipophilic toxinprofiles as a function of the shellfish sampling location. The primary finding was that all ofthe samples contained OA and acyl-OA. In addition, other lipophilic toxins were found inshellfish samples: DTX-2, acyl-DTX-2 and SPXs (SPX-A, SPX-desMeC) on the Atlanticcoast (Southern Brittany, Arcachon), and pectenotoxins (PTX-2, PTX-2-seco-acid and 7-epi-PTX-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica).This paper reports on the first detection of PTX-2, SPX-A and their derivatives in Frenchshellfish.
@article{amzil_report_2007,
	title = {Report on the First Detection of Pectenotoxin-2, Spirolide-A and Their Derivatives in French Shellfish},
	volume = {5},
	rights = {http://creativecommons.org/licenses/by/3.0/},
	url = {https://www.mdpi.com/1660-3397/5/4/168},
	doi = {10.3390/md504168},
	abstract = {In the context of the French Phytoplankton and Phycotoxins {MonitoringNetwork} ({REPHY}) programme, shellfish samples were harvested from different locationswhere harmful algae blooms were known to have occurred. For all shellfish samples foundpositive by the mouse bioassay for diarrhetic shellfish poisoning ({DSP}) toxins, liquidchromatography ({LC}) coupled with mass spectrometry ({MS}) was used to search for thefollowing lipophilic toxins: okadaic acid ({OA}), dinophysistoxins ({DTXs}), pectenotoxins({PTXs}), azaspiracids ({AZAs}), yessotoxins ({YTXs}), spirolides ({SPXs}) and gymnodimines({GYMs}). In order to investigate the presence of acyl-{OAs} and/or acyl-{DTX}-1,-2 ({DTX}-3),alkaline hydrolysis was performed on all samples, and {LC}/{MS} analyses were carried out onthe samples before and after hydrolysis. The results revealed different lipophilic toxinprofiles as a function of the shellfish sampling location. The primary finding was that all ofthe samples contained {OA} and acyl-{OA}. In addition, other lipophilic toxins were found inshellfish samples: {DTX}-2, acyl-{DTX}-2 and {SPXs} ({SPX}-A, {SPX}-{desMeC}) on the Atlanticcoast (Southern Brittany, Arcachon), and pectenotoxins ({PTX}-2, {PTX}-2-seco-acid and 7-epi-{PTX}-2-seco-acid) on the Mediterranean coast (Thau lagoon, the island of Corsica).This paper reports on the first detection of {PTX}-2, {SPX}-A and their derivatives in Frenchshellfish.},
	pages = {168--179},
	number = {4},
	journaltitle = {Marine Drugs},
	author = {Amzil, Zouher and Sibat, Manoella and Royer, Florence and Masson, Nadine and Abadie, Eric},
	urldate = {2019-04-15},
	date = {2007-12},
	langid = {english},
	note = {Number: 4},
	keywords = {{DSP} toxins, {LC}/{MS} analysis, pectenotoxins, spirolides}
}

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