Novel Family of Carbohydrate-Binding Modules Revealed by the Genome Sequence of Spirochaeta thermophila DSM 6192. Angelov, A., Loderer, C., Pompei, S., & Liebl, W. Applied and Environmental Microbiology, 77(15):5483-5489, 6, 2011. Paper Website abstract bibtex Spirochaeta thermophila is a thermophilic, free-living, and cellulolytic anaerobe. The genome sequence data for this organism have revealed a high density of genes encoding enzymes from more than 30 glycoside hydrolase (GH) families and a noncellulosomal enzyme system for (hemi)cellulose degradation. Functional screening of a fosmid library whose inserts were mapped on the S. thermophila genome sequence allowed the functional annotation of numerous GH open reading frames (ORFs). Seven different GH ORFs from the S. thermophila DSM 6192 genome, all putative β-glycanase ORFs according to sequence similarity analysis, contained a highly conserved novel GH-associated module of unknown function at their C terminus. Four of these GH enzymes were experimentally verified as xylanase, β-glucanase, β-glucanase/carboxymethylcellulase (CMCase), and CMCase. Binding experiments performed with the recombinantly expressed and purified GH-associated module showed that it represents a new carbohydrate-binding module (CBM) that binds to microcrystalline cellulose and is highly specific for this substrate. In the course of this work, the new CBM type was only detected in Spirochaeta, but recently we found sequences with detectable similarity to the module in the draft genomes of Cytophaga fermentans and Mahella australiensis, both of which are phylogenetically very distant from S. thermophila and noncellulolytic, yet inhabit similar environments. This suggests a possibly broad distribution of the module in nature.
@article{
title = {Novel Family of Carbohydrate-Binding Modules Revealed by the Genome Sequence of Spirochaeta thermophila DSM 6192},
type = {article},
year = {2011},
identifiers = {[object Object]},
keywords = {4-beta Xylanases,4-beta Xylanases: genetics,Amino Acid Sequence,Bacterial,Base Sequence,Carbohydrate Metabolism,Carbohydrates,Cell Surface,Cell Surface: chemistry,Cell Surface: genetics,Cell Surface: metabolism,Cellulase,Cellulase: genetics,Cellulose,Cellulose: metabolism,Cytophaga,Cytophaga: genetics,Cytophaga: metabolism,DNA,Endo-1,Genome,Glycoside Hydrolases,Glycoside Hydrolases: chemistry,Glycoside Hydrolases: genetics,Glycoside Hydrolases: metabolism,Phylogeny,Protein Binding,Protein Binding: genetics,Proteoglycans,Proteoglycans: metabolism,Receptors,Sequence Alignment,Sequence Analysis,Spirochaeta,Spirochaeta: genetics,Spirochaeta: metabolism,Transforming Growth Factor beta,Transforming Growth Factor beta: metabo},
pages = {5483-5489},
volume = {77},
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abstract = {Spirochaeta thermophila is a thermophilic, free-living, and cellulolytic anaerobe. The genome sequence data for this organism have revealed a high density of genes encoding enzymes from more than 30 glycoside hydrolase (GH) families and a noncellulosomal enzyme system for (hemi)cellulose degradation. Functional screening of a fosmid library whose inserts were mapped on the S. thermophila genome sequence allowed the functional annotation of numerous GH open reading frames (ORFs). Seven different GH ORFs from the S. thermophila DSM 6192 genome, all putative β-glycanase ORFs according to sequence similarity analysis, contained a highly conserved novel GH-associated module of unknown function at their C terminus. Four of these GH enzymes were experimentally verified as xylanase, β-glucanase, β-glucanase/carboxymethylcellulase (CMCase), and CMCase. Binding experiments performed with the recombinantly expressed and purified GH-associated module showed that it represents a new carbohydrate-binding module (CBM) that binds to microcrystalline cellulose and is highly specific for this substrate. In the course of this work, the new CBM type was only detected in Spirochaeta, but recently we found sequences with detectable similarity to the module in the draft genomes of Cytophaga fermentans and Mahella australiensis, both of which are phylogenetically very distant from S. thermophila and noncellulolytic, yet inhabit similar environments. This suggests a possibly broad distribution of the module in nature.},
bibtype = {article},
author = {Angelov, A. and Loderer, C. and Pompei, S. and Liebl, W.},
journal = {Applied and Environmental Microbiology},
number = {15}
}
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