Total synthesis of a functional designer eukaryotic chromosome.

Total synthesis of a functional designer eukaryotic chromosome. Annaluru, N., Muller, H., Mitchell, L. A, Ramalingam, S., Stracquadanio, G., Richardson, S. M, Dymond, J. S, Kuang, Z., Scheifele, L. Z, Cooper, E. M, Cai, Y., Zeller, K., Agmon, N., Han, J. S, Hadjithomas, M., Tullman, J., Caravelli, K., Cirelli, K., Guo, Z., London, V., Yeluru, A., Murugan, S., Kandavelou, K., Agier, N., Fischer, G., Yang, K., Martin, J A., Bilgel, M., Bohutski, P., Boulier, K. M, Capaldo, B. J, Chang, J., Charoen, K., Choi, W. J., Deng, P., DiCarlo, J. E, Doong, J., Dunn, J., Feinberg, J. I, Fernandez, C., Floria, C. E, Gladowski, D., Hadidi, P., Ishizuka, I., Jabbari, J., Lau, C. Y L, Lee, P. A, Li, S., Lin, D., Linder, M. E, Ling, J., Liu, J., Liu, J., London, M., Ma, H., Mao, J., McDade, J. E, McMillan, A., Moore, A. M, Oh, W. C., Ouyang, Y., Patel, R., Paul, M., Paulsen, L. C, Qiu, J., Rhee, A., Rubashkin, M. G, Soh, I. Y, Sotuyo, N. E, Srinivas, V., Suarez, A., Wong, A., Wong, R., Xie, W. R., Xu, Y., Yu, A. T, Koszul, R., Bader, J. S, Boeke, J. D, & Chandrasegaran, S. Science, 344(6179):55–58, April, 2014.

Paper doi abstract bibtex

Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.

@article{annaluru_total_2014,
	title = {Total synthesis of a functional designer eukaryotic chromosome.},
	volume = {344},
	url = {http://www.sciencemag.org/content/344/6179/55.full},
	doi = {10.1126/science.1249252},
	abstract = {Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomyces cerevisiae chromosome III. Changes to synIII include TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites to enable genome scrambling. SynIII is functional in S. cerevisiae. Scrambling of the chromosome in a heterozygous diploid reveals a large increase in a-mater derivatives resulting from loss of the MATα allele on synIII. The complete design and synthesis of synIII establishes S. cerevisiae as the basis for designer eukaryotic genome biology.},
	language = {English},
	number = {6179},
	journal = {Science},
	author = {Annaluru, Narayana and Muller, Héloïse and Mitchell, Leslie A and Ramalingam, Sivaprakash and Stracquadanio, Giovanni and Richardson, Sarah M and Dymond, Jessica S and Kuang, Zheng and Scheifele, Lisa Z and Cooper, Eric M and Cai, Yizhi and Zeller, Karen and Agmon, Neta and Han, Jeffrey S and Hadjithomas, Michalis and Tullman, Jennifer and Caravelli, Katrina and Cirelli, Kimberly and Guo, Zheyuan and London, Viktoriya and Yeluru, Apurva and Murugan, Sindurathy and Kandavelou, Karthikeyan and Agier, Nicolas and Fischer, Gilles and Yang, Kun and Martin, J Andrew and Bilgel, Murat and Bohutski, Pavlo and Boulier, Kristin M and Capaldo, Brian J and Chang, Joy and Charoen, Kristie and Choi, Woo Jin and Deng, Peter and DiCarlo, James E and Doong, Judy and Dunn, Jessilyn and Feinberg, Jason I and Fernandez, Christopher and Floria, Charlotte E and Gladowski, David and Hadidi, Pasha and Ishizuka, Isabel and Jabbari, Javaneh and Lau, Calvin Y L and Lee, Pablo A and Li, Sean and Lin, Denise and Linder, Matthias E and Ling, Jonathan and Liu, Jaime and Liu, Jonathan and London, Mariya and Ma, Henry and Mao, Jessica and McDade, Jessica E and McMillan, Alexandra and Moore, Aaron M and Oh, Won Chan and Ouyang, Yu and Patel, Ruchi and Paul, Marina and Paulsen, Laura C and Qiu, Judy and Rhee, Alex and Rubashkin, Matthew G and Soh, Ina Y and Sotuyo, Nathaniel E and Srinivas, Venkatesh and Suarez, Allison and Wong, Andy and Wong, Remus and Xie, Wei Rose and Xu, Yijie and Yu, Allen T and Koszul, Romain and Bader, Joel S and Boeke, Jef D and Chandrasegaran, Srinivasan},
	month = apr,
	year = {2014},
	pmid = {24674868},
	pmcid = {PMC4033833},
	pages = {55--58},
}

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