Cell and Tissue Biology, 7(6):522-527, 2013. cited By 2Paper doi abstract bibtex
ABCA1 transporter is one of the key factors defining the level of antiatherogenic HDL in plasma. It is involved in cholesterol removal from peripheral tissues by reverse cholesterol transport. However, the influence of ABCA1 mRNA and ABCA1 protein levels in macrophages on atherosclerosis remains unexplored. Using real-time PCR, we determined the ABCA1 mRNA level in macrophages cultured for 5 days with macrophage colony-stimulating factor (M-CSF). The ABCA1 mRNA level in macrophages from patients with arterial stenosis was increased compared to the control group, p = 0.04. Western-blot assayed ABCA1 protein content in macrophages from patients was significantly lower than in the control group, p = 0.01. Our results suggest that ABCA1 mRNA and ABCA1 protein levels in macrophages may be important factors in the development of atherosclerosis. © 2013 Pleiades Publishing, Ltd.