A family of shuttle vectors for lactic acid bacteria and other gram-positive bacteria based on the plasmid pLF1311 replicon. Microbiology, 69(1):63-67, 2000. cited By 1![A family of shuttle vectors for lactic acid bacteria and other gram-positive bacteria based on the plasmid pLF1311 replicon [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. The vectors are based on the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM 1311, belonging to the family of the σ-type pE194-like plasmids. The vectors can replicate in gram-positive bacteria and Escherichia coli. They are stable in many gram-positive bacteria, have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncPα can be mobilized in a great number of bacteria using a helper plasmid from E. coli but not from gram-positive bacteria. © 2000 MAIK "Nauka/Interperiodica".
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