{"_id":"qcxfJCvAvw4swuCvf","bibbaseid":"anonymous-artichokeandcynarliqueurtwonotquiteentangledproteomes-2013","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Saez"],"firstnames":["V."],"suffixes":[]},{"propositions":[],"lastnames":["Fasoli"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["D'Amato"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Simó-Alfonso"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Righetti"],"firstnames":["P.G."],"suffixes":[]}],"title":"Artichoke and Cynar liqueur: Two (not quite) entangled proteomes","journal":"Biochimica et Biophysica Acta - Proteins and Proteomics","year":"2013","volume":"1834","number":"1","pages":"119-126","doi":"10.1016/j.bbapap.2012.08.020","note":"cited By 17","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869861776&doi=10.1016%2fj.bbapap.2012.08.020&partnerID=40&md5=37f742c2c1a36553b7175793e8e716c6","affiliation":"Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain; Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, 20131 Milan, Italy; Miles Gloriosus Academy, Via Archimede 114, 20129 Milan, Italy","abstract":"Combinatorial peptide ligand libraries (CPLLs) have been adopted to investigate the proteome of artichoke extracts, of a home-made alcoholic infusion and of the Italian Cynar liqueur. The aim of study was not only to perform the deepest investigation so far of the artichoke proteome but also to assess the genuineness of the commercial aperitif via a three-pronged attack. First, different extraction techniques have been used for the characterization of the artichoke's proteome, secondly a home-made infusion has been analyzed and finally the proteome of the commercial drink was checked. The artichoke proteome has been evaluated via prior capture with CPLLs at four different pH (2.2, 4.0, 7.2 and 9.3) values. Via mass spectrometry analysis of the recovered fractions, after elution of the captured populations in 4% boiling SDS, we could identify a total of 876 unique gene products in the artichoke extracts, 18 in the home-made infusion and no proteins at all in the Italian Cynar liqueur, casting severe doubts on the procedure stated by the manufacturer (that should be made by an infusion of artichoke leaves plus thirteen different herbs). This could be the starting point for investigating the genuineness and natural origin of commercial drinks in order to protect consumers from adulterated products. © 2012 Elsevier B.V. All rights reserved.","author_keywords":"Artichoke; Combinatorial peptide ligand libraries; Cynar aperitif; Low abundance proteome; Mass spectrometry","keywords":"Cynara scolymus extract; proteome, artichoke; article; combinatorial peptide ligand library; controlled study; extraction; gene product; infusion; mass spectrometry; nonhuman; peptide library; pH; plant leaf; priority journal, Beverages; Cynara scolymus; Food Analysis; Peptide Library; Plant Extracts; Proteome, Cynara scolymus","chemicals_cas":"Peptide Library; Plant Extracts; Proteome","funding_details":"Politecnico di MilanoPolitecnico di Milano","key":"Saez2013119","id":"Saez2013119","bibbaseid":"anonymous-artichokeandcynarliqueurtwonotquiteentangledproteomes-2013","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-84869861776&doi=10.1016%2fj.bbapap.2012.08.020&partnerID=40&md5=37f742c2c1a36553b7175793e8e716c6"},"keyword":["Cynara scolymus extract; proteome","artichoke; article; combinatorial peptide ligand library; controlled study; extraction; gene product; infusion; mass spectrometry; nonhuman; peptide library; pH; plant leaf; priority journal","Beverages; Cynara scolymus; Food Analysis; Peptide Library; Plant Extracts; Proteome","Cynara scolymus"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://www.clecem.es/wp-content/uploads/2024/01/scopus.bib","dataSources":["z88YG74vvXjjYpRcn"],"keywords":["cynara scolymus extract; proteome","artichoke; article; combinatorial peptide ligand library; controlled study; extraction; gene product; infusion; mass spectrometry; nonhuman; peptide library; ph; plant leaf; priority journal","beverages; cynara scolymus; food analysis; peptide library; plant extracts; proteome","cynara scolymus"],"search_terms":["artichoke","cynar","liqueur","two","quite","entangled","proteomes"],"title":"Artichoke and Cynar liqueur: Two (not quite) entangled proteomes","year":2013}