{"_id":"jgQuNdSATxCsa8NnW","bibbaseid":"anonymous-characterizationofproliferatingcellnuclearantigenpcnaisoformsinnormalandcancercellsthereisnocancerassociatedformofpcna-2007","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Naryzhny"],"firstnames":["S.N."],"suffixes":[]},{"propositions":[],"lastnames":["Lee"],"firstnames":["H."],"suffixes":[]}],"title":"Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA","journal":"FEBS Letters","year":"2007","volume":"581","number":"25","pages":"4917-4920","doi":"10.1016/j.febslet.2007.09.022","note":"cited By 32","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34948861204&doi=10.1016%2fj.febslet.2007.09.022&partnerID=40&md5=5d65b978c1a709e4474adf89b86bc9dd","affiliation":"Tumour Biology Group, Northeastern Ontario Regional Cancer Program, the Sudbury Regional Hospital, 41 Ramsey Lake Road, Sudbury, Ont. P3E 5J1, Canada","abstract":"In order to clarify the status of PCNA in normal and transformed cells, we performed analysis of this protein by 2D-PAGE, Western blot and mass spectrometry. All the cell lines examined contained the major PCNA form (pI 4.57/30 kDa), that is not post-translationally modified. In addition to the major form, two minor isoforms (pI 4.52/30 kDa and pI 4.62/30 kDa) were also detected in all the cell lines examined. However, the level of PCNA in cancer cells is 5-6 folds higher than those in primary and most of the immortalized cells. Taken together, the significant difference in PCNA status between cancer and normal cells is not at the post-translational modifications but in the overall levels of PCNA. Crown Copyright © 2007.","author_keywords":"Cancer cell; Normal cell; PCNA; Two-dimensional gel electrophoresis","funding_details":"Canadian Cancer Society016072","key":"Naryzhny20074917","id":"Naryzhny20074917","bibbaseid":"anonymous-characterizationofproliferatingcellnuclearantigenpcnaisoformsinnormalandcancercellsthereisnocancerassociatedformofpcna-2007","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-34948861204&doi=10.1016%2fj.febslet.2007.09.022&partnerID=40&md5=5d65b978c1a709e4474adf89b86bc9dd"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lpro_2019_10.txt","dataSources":["eoAALb5LHAEShrPHa"],"keywords":[],"search_terms":["characterization","proliferating","cell","nuclear","antigen","pcna","isoforms","normal","cancer","cells","cancer","associated","form","pcna"],"title":"Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA","year":2007}