{"_id":"aAHo53xS7uShzi5WH","bibbaseid":"anonymous-determinationoflascorbicacidinlycopersiconfruitsbycapillaryzoneelectrophoresis-2001","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Galiana-Balaguer"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Roselló"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Herrero-Martínez"],"firstnames":["J.M."],"suffixes":[]},{"propositions":[],"lastnames":["Maquieira"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Nuez"],"firstnames":["F."],"suffixes":[]}],"title":"Determination of L-ascorbic acid in Lycopersicon fruits by capillary zone electrophoresis","journal":"Analytical Biochemistry","year":"2001","volume":"296","number":"2","pages":"218-224","doi":"10.1006/abio.2001.5297","note":"cited By 38","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035884579&doi=10.1006%2fabio.2001.5297&partnerID=40&md5=0ef58754f1098094775cdc83e13fdac8","affiliation":"Department of Biotechnology, Genetics, Polytechnic University of Valencia, E-46022, Valencia, Spain; Department of Chemistry, Polytechnic University of Valencia, E-46022, Valencia, Spain; Department of Analytical Chemistry, University of Valencia, Valencia, Spain","abstract":"This study shows an improved method for the determination of L-ascorbic acid (L-AA) in fruits of Lycopersicon by capillary zone electrophoresis (CZE). Two backgrounds electrolytes (BGEs) have been tested: (i) 400 mM borate at pH 8.0 and 1 × 10-2% hexadimethrine bromide, for the separation of Eulycopersicon subgenus species; and (ii) as in BGE(i) but supplemented with 20% (v/v) acetonitrile, for the separation of species of the Eriopersicon subgenus. The present procedures were compared with two routine methods-enzymatic assay and potentiometric titration with 2,6-dichlorophenol-indophenol. While these routine methods presented some difficulties in quantifying L-AA in several Lycopersicon fruits, CZE was successfully applied in all the analyzed samples. The proposed CZE protocols give lower detection limits (<0.4 μg ml-1); are cheaper, quicker, and highly reproducible; and can be applied to analyze large series of samples (ca. 50 samples per day) which is utmost importance, not only in screening trials for internal quality and tomato breeding programs, but also in systematic and routine characterization of Lycopersicon fruits. © 2001 Academic Press.","author_keywords":"Capillary zone electrophoresis; Comparison of methods; L-ascorbic acid; Lycopersicon; Screening","keywords":"Capillary electrophoresis; Fruits; Screening; Titration; Voltammetry, Breeding program; Capillary zone electrophoresis; Comparison of methods; Internal quality; L-ascorbic acid; Lower detection limit; Lycopersicon; Potentiometric titrations, Ascorbic acid, ascorbic acid; boric acid; hexadimethrine bromide; indophenol, article; capillary zone electrophoresis; enzymatic assay; fruit; intermethod comparison; Lycopersicon; potentiometric titration; priority journal; reproducibility","chemicals_cas":"ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; boric acid, 10043-35-3, 11113-50-1, 11129-12-7, 14213-97-9; hexadimethrine bromide, 28728-55-4, 9011-04-5; indophenol, 500-85-6","funding_details":"GV00-084-3","key":"Galiana-Balaguer2001218","id":"Galiana-Balaguer2001218","bibbaseid":"anonymous-determinationoflascorbicacidinlycopersiconfruitsbycapillaryzoneelectrophoresis-2001","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035884579&doi=10.1006%2fabio.2001.5297&partnerID=40&md5=0ef58754f1098094775cdc83e13fdac8"},"keyword":["Capillary electrophoresis; Fruits; Screening; Titration; Voltammetry","Breeding program; Capillary zone electrophoresis; Comparison of methods; Internal quality; L-ascorbic acid; Lower detection limit; Lycopersicon; Potentiometric titrations","Ascorbic acid","ascorbic acid; boric acid; hexadimethrine bromide; indophenol","article; capillary zone electrophoresis; enzymatic assay; fruit; intermethod comparison; Lycopersicon; potentiometric titration; priority journal; reproducibility"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://www.clecem.es/wp-content/uploads/2024/01/scopus.bib","dataSources":["z88YG74vvXjjYpRcn"],"keywords":["capillary electrophoresis; fruits; screening; titration; voltammetry","breeding program; capillary zone electrophoresis; comparison of methods; internal quality; l-ascorbic acid; lower detection limit; lycopersicon; potentiometric titrations","ascorbic acid","ascorbic acid; boric acid; hexadimethrine bromide; indophenol","article; capillary zone electrophoresis; enzymatic assay; fruit; intermethod comparison; lycopersicon; potentiometric titration; priority journal; reproducibility"],"search_terms":["determination","ascorbic","acid","lycopersicon","fruits","capillary","zone","electrophoresis"],"title":"Determination of L-ascorbic acid in Lycopersicon fruits by capillary zone electrophoresis","year":2001}