{"_id":"FYZNQpZPnQuv5yFoH","bibbaseid":"anonymous-identificationofchlamydomonasreinhardtiirad51crecombinationalcharacteristics-2005","bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Shalguev"],"firstnames":["V.I."],"suffixes":[]},{"propositions":[],"lastnames":["Kaboev"],"firstnames":["O.K."],"suffixes":[]},{"propositions":[],"lastnames":["Sizova"],"firstnames":["I.A."],"suffixes":[]},{"propositions":[],"lastnames":["Hagemann"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Lanzov"],"firstnames":["V.A."],"suffixes":[]}],"title":"Identification of Chlamydomonas reinhardtii Rad51C: Recombinational characteristics","journal":"Molecular Biology","year":"2005","volume":"39","number":"1","pages":"98-104","doi":"10.1007/s11008-005-0014-z","note":"cited By 4","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21744451037&doi=10.1007%2fs11008-005-0014-z&partnerID=40&md5=3ebe83a2b9ff2bf1c49005bb43710662","affiliation":"Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningrad Region, 188300, Russian Federation; St. Petersburg State University, St. Petersburg, 198904, Russian Federation; Institute für Biochemie, Genetik, and Microbiologie, Universität Regensburg, 93040, Germany","abstract":"Unicellular green alga Chlamydomonas reinhardtii is a promising model for fundamental and biotechnological research. However, little is known about its system of homologous recombination underlying recombination repair of double-strand breaks. Sequencing of the C. reinhardtii nuclear genome has revealed many repeats, which account for a low level of nuclear homologous recombination compared to that of nonhomologous recombination. Analysis of C. reinhardtii EST and genomic libraries made it possible to reconstruct and clone the RAD51C cDNA. In this work, this cDNA was expressed, the protein product was purified, and its main biochemical activities were studied. It was shown that Rad51C of lower eukaryote C. reinhardtii is a typical member of the subfamily of higher eukaryotic Rad51-like recombination proteins. © 2005 Pleiades Publishing, Inc.","author_keywords":"Biochemical activities of DNA transferases; Chlamydomonas reinhardtii; Homologous recombination; Rad51C","key":"Shalguev200598","id":"Shalguev200598","bibbaseid":"anonymous-identificationofchlamydomonasreinhardtiirad51crecombinationalcharacteristics-2005","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21744451037&doi=10.1007%2fs11008-005-0014-z&partnerID=40&md5=3ebe83a2b9ff2bf1c49005bb43710662"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lmkb_2019_10.txt","dataSources":["rgjRz8LhiuL9Prc4S"],"keywords":[],"search_terms":["identification","chlamydomonas","reinhardtii","rad51c","recombinational","characteristics"],"title":"Identification of Chlamydomonas reinhardtii Rad51C: Recombinational characteristics","year":2005}