Not growth associated protein GAP-43 (B-50), but its fragment GAP-43-3 (B-60) predominates in rat brain during development. Neuroscience Letters, 297(1):49-52, 2001. cited By 6![Not growth associated protein GAP-43 (B-50), but its fragment GAP-43-3 (B-60) predominates in rat brain during development [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The participation of the nerve termini growth associated protein GAP-43 in neurite outgrowth and targeting is well documented. Commonly, besides GAP-43 itself, two big fragments devoid of four (GAP-43-2, IB-50) and of about 40 (GAP-43-3, B-60) N-terminal residues were co-isolated from brain. In adult brain, GAP-43 significantly prevails over the fragments. To find their relative amounts during development, rat brain proteins were isolated on different stages of embryonal and post-natal development and subjected to gel electrophoresis in 0.9 M acetic acid-2.5 M urea system. The bands of GAP-43 protein family were detected on Western blots. We show that in developing brain (until 5th post-natal day), a proteolysis of GAP-43 near Ser41 that results in GAP-43-3 accumulation is activated. We hypothesize that just the functions that can be performed by the GAP-43 fragments are of importance for developing brain. Copyright (C) 2001 Elsevier Science Ireland Ltd.
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